Rorug05G0331200

exonuclease V

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0002756
Physical Location & Seq
Reverse (-)
40104319 .. 40105874
1556 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0331200.1

Sequence Viewer

Length: 537 bp
ATGGCTGCTCTGTCCGGCTTGGTCCTCCCAACACTTCTCTTTGCATTGTCTCTTGCAAGCTTTGAGCTCTCCAATGGCCAAGTTCTGAAGGCCAAAGTCACTTGCCTCGATTGCCACAAGAATTATGATTTTTCAGGTATTAAGGTTTTAGTGAAGTGTGCTCAAGTCAAGAAGCTGTCTATGGCAACCACAGAAGCTGACGGATCTTTCGAGGCTAAGCTCCCATCCGACGACTCGAAATCTCCCGCAGCTCTTCACTGCCTTGCCAAGATTCTTGGAGGTACAAACCAGCTCTATGTCTCAAGGAAGGTCGTGGTATCCAAGATTGTGAAGACCCATGACTCTAACTCCTACACCATTTCCACTCCTCTTGGTTTCTCCACATCATGCCCCTTGAGTATCAAAGATCAAGCGGCTTGTAAAGCCAAGAACGAGTTTGGTTCATCCAAGACCGTCGATCTACCTCTGCCGCCAGAGTGGGGTTTGGCACCTTCTAGCTTCTATGTTCCTTTCATCCCTATCATAGGCATACCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo5

Protein Analysis

178

Amino Acids

18.91

Weight (kDa)

8.87

Isoelectric Point (pI)

34.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 487
AciI CCGC 3 cut(s) 246, 413, 470
AclWI GGATC 1 cut(s) 211
AcoI YGGCCR 1 cut(s) 76
AcuI CTGAAG 1 cut(s) 107
AfaI GTAC 1 cut(s) 283
AfiI CCNNNNNNNGG 2 cut(s) 479, 524
AluBI AGCT 8 cut(s) 60, 67, 175, 197, 220, 251, 292, 498
AluI AGCT 8 cut(s) 60, 67, 175, 197, 220, 251, 292, 498
Alw21I GWGCWC 2 cut(s) 69, 163
Alw26I GTCTC 2 cut(s) 54, 304
AlwI GGATC 1 cut(s) 211
AlwNI CAGNNNCTG 1 cut(s) 197
AoxI GGCC 2 cut(s) 76, 90
ApeKI GCWGC 2 cut(s) 5, 248
ArsI GACNNNNNNTTYG 2 cut(s) 191, 223
AspS9I GGNCC 1 cut(s) 22
AvaII GGWCC 1 cut(s) 22
BalI TGGCCA 1 cut(s) 78
BanI GGYRCC 1 cut(s) 487
BanII GRGCYC 1 cut(s) 69
BbsI GAAGAC 1 cut(s) 338
Bbv12I GWGCWC 2 cut(s) 69, 163
BbvI GCAGC 1 cut(s) 260
BccI CCATC 1 cut(s) 232
BciVI GTATCC 1 cut(s) 328
BcoDI GTCTC 2 cut(s) 54, 304
BfaI CTAG 1 cut(s) 495
BfuI GTATCC 1 cut(s) 328
BisI GCNGC 4 cut(s) 6, 249, 414, 470
BlpI GCTNAGC 1 cut(s) 216
BlsI GCNGC 4 cut(s) 7, 250, 415, 471
Bme18I GGWCC 1 cut(s) 22
BmgT120I GGNCC 1 cut(s) 22
BmiI GGNNCC 1 cut(s) 489
BpiI GAAGAC 1 cut(s) 338
Bpu1102I GCTNAGC 1 cut(s) 216
BpuEI CTTGAG 3 cut(s) 147, 286, 415
BsaXI ACNNNNNCTCC 2 cut(s) 332, 362
Bsc4I CCNNNNNNNGG 2 cut(s) 479, 524
BseGI GGATG 3 cut(s) 224, 443, 513
BseLI CCNNNNNNNGG 2 cut(s) 479, 524
BseRI GAGGAG 1 cut(s) 357
BseXI GCAGC 1 cut(s) 260
BshFI GGCC 2 cut(s) 78, 92
BshNI GGYRCC 1 cut(s) 487
BsiHKAI GWGCWC 2 cut(s) 69, 163
BsiSI CCGG 1 cut(s) 15
BslI CCNNNNNNNGG 2 cut(s) 479, 524
BsmAI GTCTC 2 cut(s) 54, 304
BsnI GGCC 2 cut(s) 78, 92
Bsp1286I GDGCHC 2 cut(s) 69, 163
Bsp143I GATC 3 cut(s) 203, 406, 457
Bsp1720I GCTNAGC 1 cut(s) 216
BspACI CCGC 3 cut(s) 246, 413, 470
BspANI GGCC 2 cut(s) 78, 92
BspLI GGNNCC 1 cut(s) 489
BspPI GGATC 1 cut(s) 211
BspQI GCTCTTC 1 cut(s) 258
BspT107I GGYRCC 1 cut(s) 487
BssMI GATC 3 cut(s) 203, 406, 457
Bst4CI ACNGT 1 cut(s) 454
Bst6I CTCTTC 1 cut(s) 258
BstC8I GCNNGC 1 cut(s) 58
BstDEI CTNAG 1 cut(s) 216
BstENI CCTNNNNNAGG 1 cut(s) 522
BstF5I GGATG 3 cut(s) 224, 443, 513
BstKTI GATC 3 cut(s) 206, 409, 460
BstMAI GTCTC 2 cut(s) 54, 304
BstMBI GATC 3 cut(s) 203, 406, 457
BstMWI GCNNNNNNNGC 2 cut(s) 111, 422
BstV1I GCAGC 1 cut(s) 260
BstV2I GAAGAC 1 cut(s) 338
BstX2I RGATCY 1 cut(s) 203
BstYI RGATCY 1 cut(s) 203
BsuI GTATCC 1 cut(s) 328
BsuRI GGCC 2 cut(s) 78, 92
BtsCI GGATG 3 cut(s) 224, 443, 513
BtsI GCAGTG 1 cut(s) 256
BtsIMutI CAGTG 1 cut(s) 256
Cac8I GCNNGC 1 cut(s) 58
CaiI CAGNNNCTG 1 cut(s) 197
Cfr13I GGNCC 1 cut(s) 22
Csp6I GTAC 1 cut(s) 282
CspCI CAANNNNNGTGG 2 cut(s) 352, 387
CviAII CATG 2 cut(s) 338, 387
CviQI GTAC 1 cut(s) 282
DdeI CTNAG 1 cut(s) 216
DpnI GATC 3 cut(s) 205, 408, 459
DpnII GATC 3 cut(s) 203, 406, 457
EaeI YGGCCR 1 cut(s) 76
Eam1104I CTCTTC 1 cut(s) 258
EarI CTCTTC 1 cut(s) 258
Ecl136II GAGCTC 1 cut(s) 67
Eco24I GRGCYC 1 cut(s) 69
Eco47I GGWCC 1 cut(s) 22
Eco53kI GAGCTC 1 cut(s) 67
Eco57I CTGAAG 1 cut(s) 107
EcoICRI GAGCTC 1 cut(s) 67
EcoNI CCTNNNNNAGG 1 cut(s) 522
EcoT38I GRGCYC 1 cut(s) 69
FaeI CATG 2 cut(s) 341, 390
FaiI YATR 8 cut(s) 126, 182, 297, 339, 388, 504, 524, 530
FatI CATG 2 cut(s) 337, 386
FauI CCCGC 1 cut(s) 253
Fnu4HI GCNGC 4 cut(s) 6, 249, 414, 470
FokI GGATG 3 cut(s) 211, 430, 500
FriOI GRGCYC 1 cut(s) 69
Fsp4HI GCNGC 4 cut(s) 6, 249, 414, 470
FspBI CTAG 1 cut(s) 495
GluI GCNGC 4 cut(s) 6, 249, 414, 470
HaeIII GGCC 2 cut(s) 78, 92
HapII CCGG 1 cut(s) 15
Hin1II CATG 2 cut(s) 341, 390
HindIII AAGCTT 1 cut(s) 58
HinfI GANTC 3 cut(s) 233, 271, 341
HpaII CCGG 1 cut(s) 15
Hpy188I TCNGA 2 cut(s) 87, 229
Hpy188III TCNNGA 1 cut(s) 169
Hpy99I CGWCG 2 cut(s) 233, 458
HpyAV CCTTC 3 cut(s) 82, 301, 501
HpyCH4III ACNGT 1 cut(s) 454
HpyCH4V TGCA 2 cut(s) 44, 56
HpyF10VI GCNNNNNNNGC 2 cut(s) 111, 422
HpyF3I CTNAG 1 cut(s) 216
Hsp92II CATG 2 cut(s) 341, 390
Kzo9I GATC 3 cut(s) 203, 406, 457
LguI GCTCTTC 1 cut(s) 258
LmnI GCTCC 1 cut(s) 225
LpnPI CCDG 4 cut(s) 28, 120, 302, 486
Lsp1109I GCAGC 1 cut(s) 260
MaeI CTAG 1 cut(s) 495
MaeIII GTNAC 1 cut(s) 97
MalI GATC 3 cut(s) 205, 408, 459
MboI GATC 3 cut(s) 203, 406, 457
MboII GAAGA 2 cut(s) 245, 343
MflI RGATCY 1 cut(s) 203
MhlI GDGCHC 2 cut(s) 69, 163
MlsI TGGCCA 1 cut(s) 78
MluCI AATT 1 cut(s) 121
MluNI TGGCCA 1 cut(s) 78
MlyI GAGTC 2 cut(s) 227, 335
MmeI TCCRAC 1 cut(s) 252
MnlI CCTC 6 cut(s) 35, 116, 205, 272, 378, 474
Mox20I TGGCCA 1 cut(s) 78
MscI TGGCCA 1 cut(s) 78
MseI TTAA 1 cut(s) 141
Msp20I TGGCCA 1 cut(s) 78
MspI CCGG 1 cut(s) 15
MwoI GCNNNNNNNGC 2 cut(s) 111, 422
NdeII GATC 3 cut(s) 203, 406, 457
NlaIII CATG 2 cut(s) 341, 390
NlaIV GGNNCC 1 cut(s) 489
NmuCI GTSAC 1 cut(s) 97
PciSI GCTCTTC 1 cut(s) 258
PcsI WCGNNNNNNNCGW 1 cut(s) 207
PfeI GAWTC 1 cut(s) 271
PkrI GCNGC 4 cut(s) 7, 250, 415, 471
PleI GAGTC 2 cut(s) 227, 335
PpsI GAGTC 2 cut(s) 227, 335
Psp124BI GAGCTC 1 cut(s) 69
PspN4I GGNNCC 1 cut(s) 489
PspPI GGNCC 1 cut(s) 22
PstNI CAGNNNCTG 1 cut(s) 197
PsuI RGATCY 1 cut(s) 203
RsaI GTAC 1 cut(s) 283
RsaNI GTAC 1 cut(s) 282
SacI GAGCTC 1 cut(s) 69
SapI GCTCTTC 1 cut(s) 258
SaqAI TTAA 1 cut(s) 141
SatI GCNGC 4 cut(s) 6, 249, 414, 470
Sau3AI GATC 3 cut(s) 203, 406, 457
Sau96I GGNCC 1 cut(s) 22
SchI GAGTC 2 cut(s) 227, 335
SduI GDGCHC 2 cut(s) 69, 163
SinI GGWCC 1 cut(s) 22
SmlI CTYRAG 3 cut(s) 162, 301, 394
SmoI CTYRAG 3 cut(s) 162, 301, 394
Sse9I AATT 1 cut(s) 121
SsiI CCGC 3 cut(s) 246, 413, 470
SspMI CTAG 1 cut(s) 495
SstI GAGCTC 1 cut(s) 69
TaaI ACNGT 1 cut(s) 454
TaqI TCGA 4 cut(s) 108, 210, 236, 456
TasI AATT 1 cut(s) 121
TauI GCSGC 2 cut(s) 416, 472
TfiI GAWTC 1 cut(s) 271
Tru1I TTAA 1 cut(s) 141
Tru9I TTAA 1 cut(s) 141
TscAI CASTG 1 cut(s) 263
TseFI GTSAC 1 cut(s) 97
TseI GCWGC 2 cut(s) 5, 248
Tsp45I GTSAC 1 cut(s) 97
TspDTI ATGAA 2 cut(s) 432, 502
TspGWI ACGGA 1 cut(s) 216
TspRI CASTG 1 cut(s) 263
VpaK11BI GGWCC 1 cut(s) 22
XagI CCTNNNNNAGG 1 cut(s) 522
XspI CTAG 1 cut(s) 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.