Rroxscaffold_1G00020190

exonuclease V

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0002756
Physical Location & Seq
Reverse (-)
24535717 .. 24541783
6067 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00020190.1

Sequence Viewer

Length: 1143 bp
ATGACGGAGTCGCCTTCCGACTCACGACTCTTGGATCCTCATCATCCTCATCTTGTGCCCCAAATCCCAATTGGGATTGTGAGCGACGAAGAGATGGCCTTGCTCTTCGCTCGCTCTCTAATCCCTTCCACAGTGAGAGCATTCCCTTCCAGCGTCAGGTCTCTTCAGTACTCCATCACCCTCCTTTCCAAGAGGAGCCTTTCGGGTTGCTTCCAACCCGGTATAGAGGATTCGGGCGGGTTCAACACTACTACCCAGAAGAAGAAGGCTGAATCGCTTTTGCACCGCTTCAGGAACAAGAAGGGCTTGTCCGCCTCCGATTTTGCTGAATCGGAATGGTGTGAAAAACAAATGCTGTACGTTCTCCTGAATGGCAACAGAGTAATCAGTAAGGCTATGAAAACTGGTAGGGAGCGCCATGCAAAACTTGAAGAAGAGTTTGTAACAAAATCTAAAGTTGTTGTCAATTCAGACGAAGATAAATGGGCCCTGAGGTTTTTGAAGTTTATTACAGGCATAAATCAATTATATACTGAAGGATTGACTCGAGAGCTGCCTCTCGTAGGTTTTGCAGAAGGTATATGGATGGTGGGAGTGATTGATGAGGTTCAAATGCCTGCCACAGAAACCCAGAGAAATCCATTATTAGTTGACACAAAGACTCGTGTGAAAGATAAACTTCCTTCCGAACCACAACGGAGGAAGGGAAGGTTTCAATTGATGTTCTACAAGTATTTGTGGGATAATCTAGAAGCATCTGGATTCCCCGCCGACCAATTCTTTGATCATTTTTCCTTAAATCCTGATTGTGACTTATCTGAAGAAATCAGGGAGGTCGTCCTAGCTAAATTAGGGTATCCAGCAAAGACCCTTGATGACGTGGTGAAATACTATAGAAAATCCTGGATCCTGCTACCCCGTGCTCATGACCAACTTTTATTGAGATACGAGTTACAAAAGCATTATTCAGTAATCGGTGAAGATATATTTGCATACGACTCTGATGTGTTTAAGAAGCAACTTCAGGTTTGTCTCGAGTTCTGGCTAGGAAAGCGAGAAGCCAGTTACACACCTGAAGAAGAGCGATGGAAGTGCTACAGCTGTGAATTTGCTTCCACATGTCCTGCAGCTCCTGCTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo5

Protein Analysis

380

Amino Acids

43.68

Weight (kDa)

6.73

Isoelectric Point (pI)

49.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo5 PF09810 92 - 208 6.8e-31 Exonuclease V - a 5' deoxyribonuclease
PDDEXK_1 PF12705 153 - 375 4.3e-07 PD-(D/E)XK nuclease superfamily
Exo5 PF09810 209 - 279 1.3e-08 Exonuclease V - a 5' deoxyribonuclease
Exo5 PF09810 309 - 374 2e-11 Exonuclease V - a 5' deoxyribonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 237, 286, 312, 768
AclWI GGATC 4 cut(s) 29, 42, 901, 914
AcsI RAATTY 1 cut(s) 1106
AcuI CTGAAG 6 cut(s) 149, 274, 555, 840, 1007, 1095
AfaI GTAC 2 cut(s) 170, 359
AfiI CCNNNNNNNGG 2 cut(s) 156, 563
AflIII ACRYGT 1 cut(s) 1118
AgsI TTSAA 5 cut(s) 244, 431, 502, 611, 716
AjiI CACGTC 1 cut(s) 880
AjnI CCWGG 1 cut(s) 902
AluBI AGCT 4 cut(s) 553, 845, 1101, 1130
AluI AGCT 4 cut(s) 553, 845, 1101, 1130
Alw21I GWGCWC 1 cut(s) 925
Alw26I GTCTC 2 cut(s) 165, 1037
AlwI GGATC 4 cut(s) 29, 42, 901, 914
AlwNI CAGNNNCTG 1 cut(s) 1133
Ama87I CYCGRG 2 cut(s) 546, 1034
AoxI GGCC 2 cut(s) 96, 486
ApaI GGGCCC 1 cut(s) 490
ApeKI GCWGC 2 cut(s) 553, 1127
ApoI RAATTY 1 cut(s) 1106
ArsI GACNNNNNNTTYG 2 cut(s) 764, 796
AspLEI GCGC 1 cut(s) 417
AspS9I GGNCC 2 cut(s) 486, 487
AsuC2I CCSGG 1 cut(s) 219
AsuHPI GGTGA 3 cut(s) 169, 895, 989
AvaI CYCGRG 2 cut(s) 546, 1034
AxyI CCTNAGG 1 cut(s) 491
BaeGI GKGCMC 2 cut(s) 60, 490
BamHI GGATCC 2 cut(s) 34, 906
BanII GRGCYC 1 cut(s) 490
BauI CACGAG 1 cut(s) 663
Bbv12I GWGCWC 1 cut(s) 925
BbvI GCAGC 2 cut(s) 540, 1139
BccI CCATC 4 cut(s) 88, 182, 580, 1080
BcgI CGANNNNNNTGC 2 cut(s) 1074, 1108
BciT130I CCWGG 1 cut(s) 904
BciVI GTATCC 1 cut(s) 867
BclI TGATCA 1 cut(s) 784
BcnI CCSGG 1 cut(s) 219
BcoDI GTCTC 2 cut(s) 165, 1037
BfaI CTAG 3 cut(s) 749, 842, 1046
BfmI CTRYAG 3 cut(s) 892, 1096, 1125
BfoI RGCGCY 1 cut(s) 418
BfuI GTATCC 1 cut(s) 867
BisI GCNGC 2 cut(s) 554, 1128
BlsI GCNGC 2 cut(s) 555, 1129
BmcAI AGTACT 1 cut(s) 170
Bme1390I CCNGG 2 cut(s) 219, 904
BmeT110I CYCGRG 2 cut(s) 546, 1034
BmgBI CACGTC 1 cut(s) 880
BmgT120I GGNCC 2 cut(s) 486, 487
BmiI GGNNCC 4 cut(s) 36, 197, 488, 908
BmrFI CCNGG 2 cut(s) 219, 904
BmsI GCATC 1 cut(s) 764
BpuMI CCSGG 1 cut(s) 219
BsaBI GATNNNNATC 1 cut(s) 39
BsaI GGTCTC 1 cut(s) 165
Bsc4I CCNNNNNNNGG 2 cut(s) 156, 563
Bse1I ACTGG 2 cut(s) 409, 1062
Bse21I CCTNAGG 1 cut(s) 491
Bse8I GATNNNNATC 1 cut(s) 39
BseBI CCWGG 1 cut(s) 904
BseGI GGATG 2 cut(s) 43, 591
BseJI GATNNNNATC 1 cut(s) 39
BseLI CCNNNNNNNGG 2 cut(s) 156, 563
BseMII CTCAG 1 cut(s) 482
BseNI ACTGG 2 cut(s) 409, 1062
BseRI GAGGAG 1 cut(s) 208
BseSI GKGCMC 2 cut(s) 60, 490
BseXI GCAGC 2 cut(s) 540, 1139
BshFI GGCC 2 cut(s) 98, 488
BsiHKAI GWGCWC 1 cut(s) 925
BsiHKCI CYCGRG 2 cut(s) 546, 1034
BsiSI CCGG 1 cut(s) 219
BslI CCNNNNNNNGG 2 cut(s) 156, 563
BsmAI GTCTC 2 cut(s) 165, 1037
BsmI GAATGC 1 cut(s) 140
BsnI GGCC 2 cut(s) 98, 488
Bso31I GGTCTC 1 cut(s) 165
BsoBI CYCGRG 2 cut(s) 546, 1034
Bsp120I GGGCCC 1 cut(s) 486
Bsp1286I GDGCHC 3 cut(s) 60, 490, 925
Bsp143I GATC 3 cut(s) 34, 784, 906
BspACI CCGC 4 cut(s) 237, 286, 312, 768
BspANI GGCC 2 cut(s) 98, 488
BspCNI CTCAG 1 cut(s) 483
BspHI TCATGA 1 cut(s) 925
BspLI GGNNCC 4 cut(s) 36, 197, 488, 908
BspMAI CTGCAG 1 cut(s) 1129
BspPI GGATC 4 cut(s) 29, 42, 901, 914
BspQI GCTCTTC 2 cut(s) 110, 1074
BspTNI GGTCTC 1 cut(s) 165
BsrI ACTGG 2 cut(s) 409, 1062
BssMI GATC 3 cut(s) 34, 784, 906
BssSI CACGAG 1 cut(s) 663
Bst2BI CACGAG 1 cut(s) 663
Bst2UI CCWGG 1 cut(s) 904
Bst4CI ACNGT 1 cut(s) 133
Bst6I CTCTTC 5 cut(s) 84, 110, 168, 429, 1074
BstAPI GCANNNNNTGC 1 cut(s) 1133
BstC8I GCNNGC 2 cut(s) 112, 618
BstDEI CTNAG 1 cut(s) 491
BstENI CCTNNNNNAGG 1 cut(s) 561
BstF5I GGATG 2 cut(s) 43, 591
BstH2I RGCGCY 1 cut(s) 418
BstHHI GCGC 1 cut(s) 417
BstKTI GATC 3 cut(s) 37, 787, 909
BstMAI GTCTC 2 cut(s) 165, 1037
BstMBI GATC 3 cut(s) 34, 784, 906
BstMWI GCNNNNNNNGC 2 cut(s) 1051, 1133
BstNI CCWGG 1 cut(s) 904
BstNSI RCATGY 1 cut(s) 1122
BstSCI CCNGG 2 cut(s) 217, 902
BstSFI CTRYAG 3 cut(s) 892, 1096, 1125
BstSLI GKGCMC 2 cut(s) 60, 490
BstV1I GCAGC 2 cut(s) 540, 1139
BstX2I RGATCY 2 cut(s) 34, 906
BstYI RGATCY 2 cut(s) 34, 906
Bsu36I CCTNAGG 1 cut(s) 491
BsuI GTATCC 1 cut(s) 867
BsuRI GGCC 2 cut(s) 98, 488
BtgZI GCGATG 1 cut(s) 1099
BtrI CACGTC 1 cut(s) 880
BtsCI GGATG 2 cut(s) 43, 591
BtsIMutI CAGTG 1 cut(s) 138
Cac8I GCNNGC 2 cut(s) 112, 618
CaiI CAGNNNCTG 1 cut(s) 1133
CciI TCATGA 1 cut(s) 925
CfoI GCGC 1 cut(s) 417
Cfr13I GGNCC 2 cut(s) 486, 487
CseI GACGC 1 cut(s) 142
Csp6I GTAC 2 cut(s) 169, 358
CviAII CATG 3 cut(s) 419, 926, 1119
CviQI GTAC 2 cut(s) 169, 358
DdeI CTNAG 1 cut(s) 491
DpnI GATC 3 cut(s) 36, 786, 908
DpnII GATC 3 cut(s) 34, 784, 906
Eam1104I CTCTTC 5 cut(s) 84, 110, 168, 429, 1074
EarI CTCTTC 5 cut(s) 84, 110, 168, 429, 1074
EciI GGCGGA 1 cut(s) 301
Eco24I GRGCYC 1 cut(s) 490
Eco31I GGTCTC 1 cut(s) 165
Eco57I CTGAAG 6 cut(s) 149, 274, 555, 840, 1007, 1095
Eco81I CCTNAGG 1 cut(s) 491
Eco88I CYCGRG 2 cut(s) 546, 1034
EcoNI CCTNNNNNAGG 1 cut(s) 561
EcoO109I RGGNCCY 1 cut(s) 487
EcoRII CCWGG 1 cut(s) 902
EcoT38I GRGCYC 1 cut(s) 490
FaeI CATG 3 cut(s) 422, 929, 1122
FalI AAGNNNNNCTT 4 cut(s) 290, 322, 663, 695
FatI CATG 3 cut(s) 418, 925, 1118
FauI CCCGC 2 cut(s) 230, 775
FbaI TGATCA 1 cut(s) 784
Fnu4HI GCNGC 2 cut(s) 554, 1128
FokI GGATG 2 cut(s) 30, 598
FriOI GRGCYC 1 cut(s) 490
Fsp4HI GCNGC 2 cut(s) 554, 1128
FspBI CTAG 3 cut(s) 749, 842, 1046
GlaI GCGC 1 cut(s) 416
GluI GCNGC 2 cut(s) 554, 1128
HaeII RGCGCY 1 cut(s) 418
HaeIII GGCC 2 cut(s) 98, 488
HapII CCGG 1 cut(s) 219
HgaI GACGC 1 cut(s) 142
HhaI GCGC 1 cut(s) 417
Hin1II CATG 3 cut(s) 422, 929, 1122
Hin6I GCGC 1 cut(s) 415
HinP1I GCGC 1 cut(s) 415
HincII GTYRAC 1 cut(s) 652
HindII GTYRAC 1 cut(s) 652
HpaII CCGG 1 cut(s) 219
HphI GGTGA 3 cut(s) 169, 895, 989
Hpy166II GTNNAC 1 cut(s) 652
Hpy188I TCNGA 7 cut(s) 19, 319, 334, 472, 688, 820, 1003
Hpy188III TCNNGA 9 cut(s) 24, 292, 367, 548, 749, 759, 803, 926, 1034
Hpy8I GTNNAC 1 cut(s) 652
Hpy99I CGWCG 1 cut(s) 89
HpyCH4III ACNGT 1 cut(s) 133
HpyCH4IV ACGT 2 cut(s) 360, 879
HpyCH4V TGCA 5 cut(s) 283, 422, 572, 992, 1127
HpyF10VI GCNNNNNNNGC 2 cut(s) 1051, 1133
HpyF3I CTNAG 1 cut(s) 491
HpySE526I ACGT 2 cut(s) 360, 879
Hsp92II CATG 3 cut(s) 422, 929, 1122
HspAI GCGC 1 cut(s) 415
Ksp22I TGATCA 1 cut(s) 784
Kzo9I GATC 3 cut(s) 34, 784, 906
LguI GCTCTTC 2 cut(s) 110, 1074
LmnI GCTCC 3 cut(s) 195, 412, 1135
Lsp1109I GCAGC 2 cut(s) 540, 1139
LweI GCATC 1 cut(s) 764
MaeI CTAG 3 cut(s) 749, 842, 1046
MaeII ACGT 2 cut(s) 360, 879
MaeIII GTNAC 4 cut(s) 442, 809, 951, 1064
MalI GATC 3 cut(s) 36, 786, 908
MboI GATC 3 cut(s) 34, 784, 906
MfeI CAATTG 2 cut(s) 69, 716
MflI RGATCY 2 cut(s) 34, 906
MhlI GDGCHC 3 cut(s) 60, 490, 925
MluCI AATT 8 cut(s) 69, 466, 524, 716, 776, 848, 1106, 1138
MlyI GAGTC 6 cut(s) 14, 17, 21, 538, 655, 992
MmeI TCCRAC 2 cut(s) 42, 238
MseI TTAA 3 cut(s) 797, 1011, 1141
MspA1I CMGCKG 1 cut(s) 1101
MspI CCGG 1 cut(s) 219
MspR9I CCNGG 2 cut(s) 219, 904
MunI CAATTG 2 cut(s) 69, 716
Mva1269I GAATGC 1 cut(s) 140
MvaI CCWGG 1 cut(s) 904
MwoI GCNNNNNNNGC 2 cut(s) 1051, 1133
NciI CCSGG 1 cut(s) 219
NdeII GATC 3 cut(s) 34, 784, 906
NlaIII CATG 3 cut(s) 422, 929, 1122
NlaIV GGNNCC 4 cut(s) 36, 197, 488, 908
NmuCI GTSAC 1 cut(s) 809
NspI RCATGY 1 cut(s) 1122
PaeR7I CTCGAG 2 cut(s) 546, 1034
PagI TCATGA 1 cut(s) 925
PciI ACATGT 1 cut(s) 1118
PciSI GCTCTTC 2 cut(s) 110, 1074
PctI GAATGC 1 cut(s) 140
PfeI GAWTC 4 cut(s) 230, 272, 329, 762
PflFI GACNNNGTC 1 cut(s) 7
PfoI TCCNGGA 1 cut(s) 902
PkrI GCNGC 2 cut(s) 555, 1129
PleI GAGTC 6 cut(s) 14, 16, 21, 538, 655, 992
PpsI GAGTC 6 cut(s) 14, 16, 21, 538, 655, 992
PscI ACATGT 1 cut(s) 1118
Psp6I CCWGG 1 cut(s) 902
PspGI CCWGG 1 cut(s) 902
PspN4I GGNNCC 4 cut(s) 36, 197, 488, 908
PspOMI GGGCCC 1 cut(s) 486
PspPI GGNCC 2 cut(s) 486, 487
PstI CTGCAG 1 cut(s) 1129
PstNI CAGNNNCTG 1 cut(s) 1133
PsuI RGATCY 2 cut(s) 34, 906
PsyI GACNNNGTC 1 cut(s) 7
PvuII CAGCTG 1 cut(s) 1101
RsaI GTAC 2 cut(s) 170, 359
RsaNI GTAC 2 cut(s) 169, 358
SapI GCTCTTC 2 cut(s) 110, 1074
SaqAI TTAA 3 cut(s) 797, 1011, 1141
SatI GCNGC 2 cut(s) 554, 1128
Sau3AI GATC 3 cut(s) 34, 784, 906
Sau96I GGNCC 2 cut(s) 486, 487
ScaI AGTACT 1 cut(s) 170
SchI GAGTC 6 cut(s) 14, 17, 21, 538, 655, 992
ScrFI CCNGG 2 cut(s) 219, 904
SduI GDGCHC 3 cut(s) 60, 490, 925
SfaNI GCATC 1 cut(s) 764
SfcI CTRYAG 3 cut(s) 892, 1096, 1125
Sfr274I CTCGAG 2 cut(s) 546, 1034
SlaI CTCGAG 2 cut(s) 546, 1034
SmlI CTYRAG 2 cut(s) 546, 1034
SmoI CTYRAG 2 cut(s) 546, 1034
Sse9I AATT 8 cut(s) 69, 466, 524, 716, 776, 848, 1106, 1138
SsiI CCGC 4 cut(s) 237, 286, 312, 768
SspMI CTAG 3 cut(s) 749, 842, 1046
StyD4I CCNGG 2 cut(s) 217, 902
TaaI ACNGT 1 cut(s) 133
TaiI ACGT 2 cut(s) 363, 882
TaqI TCGA 2 cut(s) 547, 1035
TasI AATT 8 cut(s) 69, 466, 524, 716, 776, 848, 1106, 1138
TatI WGTACW 1 cut(s) 168
TfiI GAWTC 4 cut(s) 230, 272, 329, 762
Tru1I TTAA 3 cut(s) 797, 1011, 1141
Tru9I TTAA 3 cut(s) 797, 1011, 1141
TscAI CASTG 1 cut(s) 138
TseFI GTSAC 1 cut(s) 809
TseI GCWGC 2 cut(s) 553, 1127
Tsp45I GTSAC 1 cut(s) 809
TspDTI ATGAA 1 cut(s) 413
TspGWI ACGGA 2 cut(s) 20, 712
TspRI CASTG 1 cut(s) 138
Tth111I GACNNNGTC 1 cut(s) 7
XagI CCTNNNNNAGG 1 cut(s) 561
XapI RAATTY 1 cut(s) 1106
XbaI TCTAGA 1 cut(s) 748
XceI RCATGY 1 cut(s) 1122
XcmI CCANNNNNNNNNTGG 1 cut(s) 68
XhoI CTCGAG 2 cut(s) 546, 1034
XspI CTAG 3 cut(s) 749, 842, 1046
ZrmI AGTACT 1 cut(s) 170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.