Rh5AG395600

exonuclease V

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0002756
Physical Location & Seq
Forward (+)
68549412 .. 68553441
4030 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG395600.1

Sequence Viewer

Length: 990 bp
ATGACCCAGTCGCCTTCCGACTCACTCCTCCATCATCATCCTCGTCCTCATCTTGTGCCCGAAATCCCAATTGAGATTGTAAGCGACGAAGAGATGGCTCTGCTCGAAGCCGCGTTGCTCTCCGCTCGCTCTCTAATCCCTTCCACAGTGAGAGCATTCCCTTCATTTCGATCCTCTCACTTCCCTTCCAATGCCAGGTCTATTCAGTCCATCACCCTCGTTTCCAAGAAGAGATTTTCGGGTTGCTCCCAACCCGATATTGAGGATTCGGGTTTAGGGTTCGACACCACCCAGAAGAAGAAGACCCGGTTGGCTGAGTCGTTTTTGCACCGCTTCAGGAGGAAGAAGGGCTTGTGCGTCACCGATTTTACTTCCGCGGAATGGTGTGAAAAACAGATGGAGTTTGAGCTCCTCGGCGGCAAGAAAAAAATTAATAAGGCTATGAAAGCTGGTAGTAAGCGCCATGCAAAACTCGAAGAAGAGGTTCTAACAAGAGTTAAAGTTGATATCAAATCAGACGAAGATAAATTTGCTCTGAAGCTTTTGAATTGTATTACCGGCATAAATCAATTACAATTTGAAGGATTGACTCGAGAGCTGCCTCTCATAGGTTTTGTAGAAGGTGTATGGATGGTGGGAGTGATTGATGAGGTTCAAATGCCTGCAACAGAAACCCAGAGAAATCCATTATTAGTTGACACAAAGACTCGTGTGAAAGATACACTTCCTGCTGAACCACAACGAAGGAATGGAAGGCTTCAATTGATGTGCTACAAGTATATGTGGGACAATTTAGTTTCTTCTGAATTCCCCTCCGACCAATTCTTTGATTATTTTTCCTTAAATCCTGATTGTGACTTATCTGAAGAAATCAGGGAGGTCACAGCTAAATCAGGGTGTCCAGCAAAGACCCTTGATGACGTAGTGAGATACTATAGAAATACCTGGAGAATGCTACCCCCTGCTCATGACCAACTTTTATTGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo5

Protein Analysis

329

Amino Acids

37.6

Weight (kDa)

8.1

Isoelectric Point (pI)

50.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo5 PF09810 107 - 223 8.7e-37 Exonuclease V - a 5' deoxyribonuclease
Exo5 PF09810 224 - 292 4.6e-08 Exonuclease V - a 5' deoxyribonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 125
AccII CGCG 2 cut(s) 113, 377
AciI CCGC 6 cut(s) 111, 123, 331, 375, 377, 417
AclWI GGATC 1 cut(s) 165
AcsI RAATTY 2 cut(s) 527, 806
AcuI CTGAAG 3 cut(s) 319, 557, 885
AfiI CCNNNNNNNGG 3 cut(s) 195, 381, 608
AgsI TTSAA 4 cut(s) 547, 581, 656, 761
AjnI CCWGG 2 cut(s) 194, 944
AjuI GAANNNNNNNTTGG 2 cut(s) 293, 325
AluBI AGCT 5 cut(s) 409, 449, 541, 598, 887
AluI AGCT 5 cut(s) 409, 449, 541, 598, 887
Alw21I GWGCWC 1 cut(s) 411
AlwI GGATC 1 cut(s) 165
Ama87I CYCGRG 1 cut(s) 591
ApeKI GCWGC 1 cut(s) 598
ApoI RAATTY 2 cut(s) 527, 806
ArsI GACNNNNNNTTYG 2 cut(s) 809, 841
AseI ATTAAT 1 cut(s) 432
Asp700I GAANNNNTTC 1 cut(s) 483
AspLEI GCGC 1 cut(s) 462
AsuC2I CCSGG 1 cut(s) 307
AsuHPI GGTGA 2 cut(s) 205, 352
AvaI CYCGRG 1 cut(s) 591
BaeGI GKGCMC 1 cut(s) 60
BanII GRGCYC 1 cut(s) 411
BauI CACGAG 1 cut(s) 708
BbsI GAAGAC 1 cut(s) 308
Bbv12I GWGCWC 1 cut(s) 411
BbvI GCAGC 1 cut(s) 585
BccI CCATC 5 cut(s) 39, 88, 218, 391, 625
BciT130I CCWGG 2 cut(s) 196, 946
BcnI CCSGG 1 cut(s) 307
BfmI CTRYAG 1 cut(s) 934
BfoI RGCGCY 1 cut(s) 463
BisI GCNGC 3 cut(s) 111, 418, 599
BlsI GCNGC 3 cut(s) 112, 419, 600
Bme1390I CCNGG 3 cut(s) 196, 307, 946
BmeT110I CYCGRG 1 cut(s) 591
BmrFI CCNGG 3 cut(s) 196, 307, 946
BpiI GAAGAC 1 cut(s) 308
BpmI CTGGAG 1 cut(s) 967
BpuMI CCSGG 1 cut(s) 307
BsaJI CCNNGG 2 cut(s) 375, 412
Bsc4I CCNNNNNNNGG 3 cut(s) 195, 381, 608
Bse118I RCCGGY 1 cut(s) 557
Bse1I ACTGG 1 cut(s) 7
BseBI CCWGG 2 cut(s) 196, 946
BseDI CCNNGG 2 cut(s) 375, 412
BseGI GGATG 2 cut(s) 37, 636
BseLI CCNNNNNNNGG 3 cut(s) 195, 381, 608
BseMII CTCAG 1 cut(s) 306
BseNI ACTGG 1 cut(s) 7
BseRI GAGGAG 2 cut(s) 17, 401
BseSI GKGCMC 1 cut(s) 60
BseXI GCAGC 1 cut(s) 585
Bsh1236I CGCG 2 cut(s) 113, 377
BsiHKAI GWGCWC 1 cut(s) 411
BsiHKCI CYCGRG 1 cut(s) 591
BsiSI CCGG 2 cut(s) 307, 558
BslFI GGGAC 1 cut(s) 800
BslI CCNNNNNNNGG 3 cut(s) 195, 381, 608
BsmFI GGGAC 1 cut(s) 800
BsmI GAATGC 2 cut(s) 155, 957
BsoBI CYCGRG 1 cut(s) 591
Bsp1286I GDGCHC 2 cut(s) 60, 411
Bsp143I GATC 1 cut(s) 170
BspACI CCGC 6 cut(s) 111, 123, 331, 375, 377, 417
BspCNI CTCAG 1 cut(s) 307
BspFNI CGCG 2 cut(s) 113, 377
BspHI TCATGA 1 cut(s) 967
BspPI GGATC 1 cut(s) 165
BsrBI CCGCTC 1 cut(s) 125
BsrFI RCCGGY 1 cut(s) 557
BsrI ACTGG 1 cut(s) 7
BssAI RCCGGY 1 cut(s) 557
BssECI CCNNGG 2 cut(s) 375, 412
BssMI GATC 1 cut(s) 170
BssSI CACGAG 1 cut(s) 708
Bst2BI CACGAG 1 cut(s) 708
Bst2UI CCWGG 2 cut(s) 196, 946
Bst4CI ACNGT 1 cut(s) 148
Bst6I CTCTTC 3 cut(s) 84, 224, 474
BstC8I GCNNGC 2 cut(s) 127, 663
BstDEI CTNAG 1 cut(s) 315
BstDSI CCRYGG 1 cut(s) 375
BstENI CCTNNNNNAGG 1 cut(s) 606
BstF5I GGATG 2 cut(s) 37, 636
BstFNI CGCG 2 cut(s) 113, 377
BstH2I RGCGCY 1 cut(s) 463
BstHHI GCGC 1 cut(s) 462
BstKTI GATC 1 cut(s) 173
BstMBI GATC 1 cut(s) 170
BstMWI GCNNNNNNNGC 1 cut(s) 446
BstNI CCWGG 2 cut(s) 196, 946
BstSCI CCNGG 3 cut(s) 194, 305, 944
BstSFI CTRYAG 1 cut(s) 934
BstSLI GKGCMC 1 cut(s) 60
BstUI CGCG 2 cut(s) 113, 377
BstV1I GCAGC 1 cut(s) 585
BstV2I GAAGAC 1 cut(s) 308
BtgI CCRYGG 1 cut(s) 375
BtsCI GGATG 2 cut(s) 37, 636
BtsIMutI CAGTG 1 cut(s) 153
Cac8I GCNNGC 2 cut(s) 127, 663
CciI TCATGA 1 cut(s) 967
CfoI GCGC 1 cut(s) 462
Cfr10I RCCGGY 1 cut(s) 557
Cfr42I CCGCGG 1 cut(s) 378
CseI GACGC 1 cut(s) 346
CviAII CATG 2 cut(s) 464, 968
DdeI CTNAG 1 cut(s) 315
DpnI GATC 1 cut(s) 172
DpnII GATC 1 cut(s) 170
Eam1104I CTCTTC 3 cut(s) 84, 224, 474
EarI CTCTTC 3 cut(s) 84, 224, 474
Ecl136II GAGCTC 1 cut(s) 409
Eco24I GRGCYC 1 cut(s) 411
Eco32I GATATC 1 cut(s) 508
Eco53kI GAGCTC 1 cut(s) 409
Eco57I CTGAAG 3 cut(s) 319, 557, 885
Eco88I CYCGRG 1 cut(s) 591
EcoICRI GAGCTC 1 cut(s) 409
EcoNI CCTNNNNNAGG 1 cut(s) 606
EcoRI GAATTC 1 cut(s) 806
EcoRII CCWGG 2 cut(s) 194, 944
EcoRV GATATC 1 cut(s) 508
EcoT38I GRGCYC 1 cut(s) 411
FaeI CATG 2 cut(s) 467, 971
FaiI YATR 9 cut(s) 443, 465, 563, 608, 628, 780, 782, 936, 969
FalI AAGNNNNNCTT 4 cut(s) 335, 367, 708, 740
FaqI GGGAC 1 cut(s) 800
FatI CATG 2 cut(s) 463, 967
Fnu4HI GCNGC 3 cut(s) 111, 418, 599
FokI GGATG 2 cut(s) 24, 643
FriOI GRGCYC 1 cut(s) 411
Fsp4HI GCNGC 3 cut(s) 111, 418, 599
GlaI GCGC 1 cut(s) 461
GluI GCNGC 3 cut(s) 111, 418, 599
GsuI CTGGAG 1 cut(s) 967
HaeII RGCGCY 1 cut(s) 463
HapII CCGG 2 cut(s) 307, 558
HgaI GACGC 1 cut(s) 346
HhaI GCGC 1 cut(s) 462
Hin1II CATG 2 cut(s) 467, 971
Hin6I GCGC 1 cut(s) 460
HinP1I GCGC 1 cut(s) 460
HincII GTYRAC 1 cut(s) 697
HindII GTYRAC 1 cut(s) 697
HindIII AAGCTT 1 cut(s) 539
HinfI GANTC 5 cut(s) 20, 266, 317, 589, 706
HpaII CCGG 2 cut(s) 307, 558
HphI GGTGA 2 cut(s) 205, 352
Hpy166II GTNNAC 1 cut(s) 697
Hpy188I TCNGA 6 cut(s) 19, 517, 537, 805, 817, 865
Hpy188III TCNNGA 4 cut(s) 337, 593, 848, 968
Hpy8I GTNNAC 1 cut(s) 697
Hpy99I CGWCG 1 cut(s) 89
HpyAV CCTTC 9 cut(s) 24, 150, 171, 195, 340, 575, 614, 738, 747
HpyCH4III ACNGT 1 cut(s) 148
HpyCH4IV ACGT 1 cut(s) 921
HpyCH4V TGCA 3 cut(s) 328, 467, 665
HpyF10VI GCNNNNNNNGC 1 cut(s) 446
HpyF3I CTNAG 1 cut(s) 315
HpySE526I ACGT 1 cut(s) 921
Hsp92II CATG 2 cut(s) 467, 971
HspAI GCGC 1 cut(s) 460
KspI CCGCGG 1 cut(s) 378
Kzo9I GATC 1 cut(s) 170
LmnI GCTCC 2 cut(s) 251, 414
Lsp1109I GCAGC 1 cut(s) 585
MaeII ACGT 1 cut(s) 921
MaeIII GTNAC 3 cut(s) 358, 854, 880
MalI GATC 1 cut(s) 172
MbiI CCGCTC 1 cut(s) 125
MboI GATC 1 cut(s) 170
MfeI CAATTG 2 cut(s) 69, 761
MhlI GDGCHC 2 cut(s) 60, 411
MlyI GAGTC 4 cut(s) 14, 326, 583, 700
MmeI TCCRAC 2 cut(s) 42, 840
MroXI GAANNNNTTC 1 cut(s) 483
MseI TTAA 3 cut(s) 432, 498, 842
MspA1I CMGCKG 1 cut(s) 377
MspI CCGG 2 cut(s) 307, 558
MspR9I CCNGG 3 cut(s) 196, 307, 946
MunI CAATTG 2 cut(s) 69, 761
Mva1269I GAATGC 2 cut(s) 155, 957
MvaI CCWGG 2 cut(s) 196, 946
MvnI CGCG 2 cut(s) 113, 377
MwoI GCNNNNNNNGC 1 cut(s) 446
NciI CCSGG 1 cut(s) 307
NdeII GATC 1 cut(s) 170
NlaIII CATG 2 cut(s) 467, 971
NmeAIII GCCGAG 1 cut(s) 393
NmuCI GTSAC 3 cut(s) 358, 854, 880
PaeR7I CTCGAG 1 cut(s) 591
PagI TCATGA 1 cut(s) 967
PctI GAATGC 2 cut(s) 155, 957
PdmI GAANNNNTTC 1 cut(s) 483
PfeI GAWTC 1 cut(s) 266
PflFI GACNNNGTC 1 cut(s) 7
PkrI GCNGC 3 cut(s) 112, 419, 600
PleI GAGTC 4 cut(s) 14, 325, 583, 700
PpsI GAGTC 4 cut(s) 14, 325, 583, 700
PshBI ATTAAT 1 cut(s) 432
Psp124BI GAGCTC 1 cut(s) 411
Psp6I CCWGG 2 cut(s) 194, 944
PspGI CCWGG 2 cut(s) 194, 944
PsyI GACNNNGTC 1 cut(s) 7
SacI GAGCTC 1 cut(s) 411
SacII CCGCGG 1 cut(s) 378
SaqAI TTAA 3 cut(s) 432, 498, 842
SatI GCNGC 3 cut(s) 111, 418, 599
Sau3AI GATC 1 cut(s) 170
SchI GAGTC 4 cut(s) 14, 326, 583, 700
ScrFI CCNGG 3 cut(s) 196, 307, 946
SduI GDGCHC 2 cut(s) 60, 411
SfcI CTRYAG 1 cut(s) 934
Sfr274I CTCGAG 1 cut(s) 591
Sfr303I CCGCGG 1 cut(s) 378
SgrBI CCGCGG 1 cut(s) 378
SlaI CTCGAG 1 cut(s) 591
SmlI CTYRAG 1 cut(s) 591
SmoI CTYRAG 1 cut(s) 591
SsiI CCGC 6 cut(s) 111, 123, 331, 375, 377, 417
SstI GAGCTC 1 cut(s) 411
StyD4I CCNGG 3 cut(s) 194, 305, 944
TaaI ACNGT 1 cut(s) 148
TaiI ACGT 1 cut(s) 924
TaqI TCGA 5 cut(s) 105, 169, 282, 474, 592
TauI GCSGC 2 cut(s) 113, 420
TfiI GAWTC 1 cut(s) 266
Tru1I TTAA 3 cut(s) 432, 498, 842
Tru9I TTAA 3 cut(s) 432, 498, 842
TscAI CASTG 1 cut(s) 153
TseFI GTSAC 3 cut(s) 358, 854, 880
TseI GCWGC 1 cut(s) 598
Tsp45I GTSAC 3 cut(s) 358, 854, 880
TspDTI ATGAA 2 cut(s) 153, 458
TspRI CASTG 1 cut(s) 153
Tth111I GACNNNGTC 1 cut(s) 7
VspI ATTAAT 1 cut(s) 432
XagI CCTNNNNNAGG 1 cut(s) 606
XapI RAATTY 2 cut(s) 527, 806
XhoI CTCGAG 1 cut(s) 591
XmnI GAANNNNTTC 1 cut(s) 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.