Rh5DG422800

exonuclease V

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0002756
Physical Location & Seq
Forward (+)
67453699 .. 67457783
4085 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG422800.1

Sequence Viewer

Length: 813 bp
ATGACCCAGTCGCCTTCCGACTCACTCCTCAATCATCATCCTCGTCCTCATCTTGTGCCCGAAATCCCAATTGAGATCGTAAGCGACGAAGAGATGGCTCTTCTCGAAGCCGCCTTGCTCTCCGCTCGCTCTCTAATCCCTTCCACAGTGAGAGCATTCCCTTCATTTCGATCCTCTCACTTCCCTTCCAATGCCAGGTCTATTCAGTCCATCACCCTCGTTTCCAAGAAGAGATTTTCGGGTTGCTCCGAACCCGATATTGAGGATTCGGGTTTAGGGTTCGACACTAGCCAGAAGAAGACCCGGTTGGCTGAGTCGTTTTTGCACCGCTTCAGGAGGAAGAAGGGCTTGTGCGTCACCGATTTTACTTCCGCGGAATGGTGTGAAAAACAGATGGAGTTTGAGCTCCTCGGCGGCAAGAAAAAAATTAATAAGGCTATGAAAGCTGGTAGTAAGCGCCATGCAAAACTCGAAGAAGAGGTTCTAACAAGAGTTAAAGTTGATATCAAATCAGACGAAGATAAATTTGCTCTGAAGCTTTTGAATTGTATTACCGGCATAAATCAATTACAATTTGAAGGATTGACTCGAGAGCTGCCTCTCATAGGTTTTGTAGAAGGTGTATGGATGGTGGGAGTGATTGATGAGGTTCAAATGCCTGCAACAGAAACCCAGAGAAATCCATTATTAGTTGACACAAAGACTCGTGTGAAAGATACACTTCCTGCTGAACCACAACGAAGGAATGGAAGGTATGCACTTCTATTGGCTTATGTTTCATTGCCATTGATGAGAAAAGGCCCGAAAATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo5

Protein Analysis

270

Amino Acids

30.4

Weight (kDa)

9.23

Isoelectric Point (pI)

49.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo5 PF09810 106 - 223 5.4e-37 Exonuclease V - a 5' deoxyribonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 125
AccII CGCG 1 cut(s) 374
AciI CCGC 6 cut(s) 111, 123, 328, 372, 374, 414
AclWI GGATC 1 cut(s) 165
AcsI RAATTY 1 cut(s) 524
AcuI CTGAAG 2 cut(s) 316, 554
AfiI CCNNNNNNNGG 3 cut(s) 195, 378, 605
AgsI TTSAA 3 cut(s) 544, 578, 653
AjnI CCWGG 1 cut(s) 194
AjuI GAANNNNNNNTTGG 2 cut(s) 290, 322
AluBI AGCT 4 cut(s) 406, 446, 538, 595
AluI AGCT 4 cut(s) 406, 446, 538, 595
Alw21I GWGCWC 1 cut(s) 408
AlwI GGATC 1 cut(s) 165
Ama87I CYCGRG 1 cut(s) 588
AoxI GGCC 1 cut(s) 799
ApeKI GCWGC 1 cut(s) 595
ApoI RAATTY 1 cut(s) 524
AseI ATTAAT 1 cut(s) 429
Asp700I GAANNNNTTC 1 cut(s) 480
AspLEI GCGC 1 cut(s) 459
AspS9I GGNCC 1 cut(s) 800
AsuC2I CCSGG 1 cut(s) 304
AsuHPI GGTGA 2 cut(s) 205, 349
AvaI CYCGRG 1 cut(s) 588
BaeGI GKGCMC 1 cut(s) 60
BanII GRGCYC 1 cut(s) 408
BauI CACGAG 1 cut(s) 705
BbsI GAAGAC 1 cut(s) 305
Bbv12I GWGCWC 1 cut(s) 408
BbvI GCAGC 1 cut(s) 582
BccI CCATC 4 cut(s) 88, 218, 388, 622
BciT130I CCWGG 1 cut(s) 196
BcnI CCSGG 1 cut(s) 304
BfaI CTAG 1 cut(s) 288
BfoI RGCGCY 1 cut(s) 460
BisI GCNGC 3 cut(s) 111, 415, 596
BlsI GCNGC 3 cut(s) 112, 416, 597
Bme1390I CCNGG 2 cut(s) 196, 304
BmeT110I CYCGRG 1 cut(s) 588
BmgT120I GGNCC 1 cut(s) 800
BmrFI CCNGG 2 cut(s) 196, 304
BpiI GAAGAC 1 cut(s) 305
BpuMI CCSGG 1 cut(s) 304
BsaJI CCNNGG 2 cut(s) 372, 409
Bsc4I CCNNNNNNNGG 3 cut(s) 195, 378, 605
Bse118I RCCGGY 1 cut(s) 554
Bse1I ACTGG 1 cut(s) 7
Bse3DI GCAATG 1 cut(s) 779
BseBI CCWGG 1 cut(s) 196
BseDI CCNNGG 2 cut(s) 372, 409
BseGI GGATG 2 cut(s) 37, 633
BseLI CCNNNNNNNGG 3 cut(s) 195, 378, 605
BseMI GCAATG 1 cut(s) 779
BseMII CTCAG 1 cut(s) 303
BseNI ACTGG 1 cut(s) 7
BseRI GAGGAG 2 cut(s) 17, 398
BseSI GKGCMC 1 cut(s) 60
BseXI GCAGC 1 cut(s) 582
Bsh1236I CGCG 1 cut(s) 374
BshFI GGCC 1 cut(s) 801
BsiHKAI GWGCWC 1 cut(s) 408
BsiHKCI CYCGRG 1 cut(s) 588
BsiSI CCGG 2 cut(s) 304, 555
BslI CCNNNNNNNGG 3 cut(s) 195, 378, 605
BsmI GAATGC 1 cut(s) 155
BsnI GGCC 1 cut(s) 801
BsoBI CYCGRG 1 cut(s) 588
Bsp1286I GDGCHC 2 cut(s) 60, 408
Bsp143I GATC 2 cut(s) 75, 170
BspACI CCGC 6 cut(s) 111, 123, 328, 372, 374, 414
BspANI GGCC 1 cut(s) 801
BspCNI CTCAG 1 cut(s) 304
BspFNI CGCG 1 cut(s) 374
BspPI GGATC 1 cut(s) 165
BspQI GCTCTTC 1 cut(s) 105
BsrBI CCGCTC 1 cut(s) 125
BsrDI GCAATG 1 cut(s) 779
BsrFI RCCGGY 1 cut(s) 554
BsrI ACTGG 1 cut(s) 7
BssAI RCCGGY 1 cut(s) 554
BssECI CCNNGG 2 cut(s) 372, 409
BssMI GATC 2 cut(s) 75, 170
BssSI CACGAG 1 cut(s) 705
Bst2BI CACGAG 1 cut(s) 705
Bst2UI CCWGG 1 cut(s) 196
Bst4CI ACNGT 1 cut(s) 148
Bst6I CTCTTC 4 cut(s) 84, 105, 224, 471
BstC8I GCNNGC 2 cut(s) 127, 660
BstDEI CTNAG 1 cut(s) 312
BstDSI CCRYGG 1 cut(s) 372
BstENI CCTNNNNNAGG 1 cut(s) 603
BstF5I GGATG 2 cut(s) 37, 633
BstFNI CGCG 1 cut(s) 374
BstH2I RGCGCY 1 cut(s) 460
BstHHI GCGC 1 cut(s) 459
BstKTI GATC 2 cut(s) 78, 173
BstMBI GATC 2 cut(s) 75, 170
BstMWI GCNNNNNNNGC 1 cut(s) 443
BstNI CCWGG 1 cut(s) 196
BstSCI CCNGG 2 cut(s) 194, 302
BstSLI GKGCMC 1 cut(s) 60
BstUI CGCG 1 cut(s) 374
BstV1I GCAGC 1 cut(s) 582
BstV2I GAAGAC 1 cut(s) 305
BsuRI GGCC 1 cut(s) 801
BtgI CCRYGG 1 cut(s) 372
BtsCI GGATG 2 cut(s) 37, 633
BtsIMutI CAGTG 1 cut(s) 153
Cac8I GCNNGC 2 cut(s) 127, 660
CfoI GCGC 1 cut(s) 459
Cfr10I RCCGGY 1 cut(s) 554
Cfr13I GGNCC 1 cut(s) 800
Cfr42I CCGCGG 1 cut(s) 375
CseI GACGC 1 cut(s) 343
CviAII CATG 1 cut(s) 461
DdeI CTNAG 1 cut(s) 312
DpnI GATC 2 cut(s) 77, 172
DpnII GATC 2 cut(s) 75, 170
Eam1104I CTCTTC 4 cut(s) 84, 105, 224, 471
EarI CTCTTC 4 cut(s) 84, 105, 224, 471
Ecl136II GAGCTC 1 cut(s) 406
Eco24I GRGCYC 1 cut(s) 408
Eco32I GATATC 1 cut(s) 505
Eco53kI GAGCTC 1 cut(s) 406
Eco57I CTGAAG 2 cut(s) 316, 554
Eco88I CYCGRG 1 cut(s) 588
EcoICRI GAGCTC 1 cut(s) 406
EcoNI CCTNNNNNAGG 1 cut(s) 603
EcoRII CCWGG 1 cut(s) 194
EcoRV GATATC 1 cut(s) 505
EcoT38I GRGCYC 1 cut(s) 408
FaeI CATG 1 cut(s) 464
FaiI YATR 8 cut(s) 440, 462, 560, 605, 625, 756, 774, 811
FalI AAGNNNNNCTT 4 cut(s) 332, 364, 705, 737
FatI CATG 1 cut(s) 460
Fnu4HI GCNGC 3 cut(s) 111, 415, 596
FokI GGATG 2 cut(s) 24, 640
FriOI GRGCYC 1 cut(s) 408
Fsp4HI GCNGC 3 cut(s) 111, 415, 596
FspBI CTAG 1 cut(s) 288
GlaI GCGC 1 cut(s) 458
GluI GCNGC 3 cut(s) 111, 415, 596
HaeII RGCGCY 1 cut(s) 460
HaeIII GGCC 1 cut(s) 801
HapII CCGG 2 cut(s) 304, 555
HgaI GACGC 1 cut(s) 343
HhaI GCGC 1 cut(s) 459
Hin1II CATG 1 cut(s) 464
Hin6I GCGC 1 cut(s) 457
HinP1I GCGC 1 cut(s) 457
HincII GTYRAC 1 cut(s) 694
HindII GTYRAC 1 cut(s) 694
HindIII AAGCTT 1 cut(s) 536
HinfI GANTC 5 cut(s) 20, 266, 314, 586, 703
HpaII CCGG 2 cut(s) 304, 555
HphI GGTGA 2 cut(s) 205, 349
Hpy166II GTNNAC 1 cut(s) 694
Hpy188I TCNGA 4 cut(s) 19, 250, 514, 534
Hpy188III TCNNGA 3 cut(s) 104, 334, 590
Hpy8I GTNNAC 1 cut(s) 694
Hpy99I CGWCG 1 cut(s) 89
HpyAV CCTTC 9 cut(s) 24, 150, 171, 195, 337, 572, 611, 735, 744
HpyCH4III ACNGT 1 cut(s) 148
HpyCH4V TGCA 4 cut(s) 325, 464, 662, 758
HpyF10VI GCNNNNNNNGC 1 cut(s) 443
HpyF3I CTNAG 1 cut(s) 312
Hsp92II CATG 1 cut(s) 464
HspAI GCGC 1 cut(s) 457
KspI CCGCGG 1 cut(s) 375
Kzo9I GATC 2 cut(s) 75, 170
LguI GCTCTTC 1 cut(s) 105
LmnI GCTCC 2 cut(s) 251, 411
Lsp1109I GCAGC 1 cut(s) 582
MaeI CTAG 1 cut(s) 288
MaeIII GTNAC 1 cut(s) 355
MalI GATC 2 cut(s) 77, 172
MbiI CCGCTC 1 cut(s) 125
MboI GATC 2 cut(s) 75, 170
MboII GAAGA 9 cut(s) 92, 101, 241, 307, 310, 352, 485, 488, 530
MfeI CAATTG 1 cut(s) 69
MhlI GDGCHC 2 cut(s) 60, 408
MluCI AATT 6 cut(s) 69, 426, 524, 544, 566, 572
MlyI GAGTC 4 cut(s) 14, 323, 580, 697
MmeI TCCRAC 1 cut(s) 42
MroXI GAANNNNTTC 1 cut(s) 480
MseI TTAA 2 cut(s) 429, 495
MspA1I CMGCKG 1 cut(s) 374
MspI CCGG 2 cut(s) 304, 555
MspR9I CCNGG 2 cut(s) 196, 304
MunI CAATTG 1 cut(s) 69
Mva1269I GAATGC 1 cut(s) 155
MvaI CCWGG 1 cut(s) 196
MvnI CGCG 1 cut(s) 374
MwoI GCNNNNNNNGC 1 cut(s) 443
NciI CCSGG 1 cut(s) 304
NdeII GATC 2 cut(s) 75, 170
NlaIII CATG 1 cut(s) 464
NmeAIII GCCGAG 1 cut(s) 390
NmuCI GTSAC 1 cut(s) 355
PaeR7I CTCGAG 1 cut(s) 588
PciSI GCTCTTC 1 cut(s) 105
PcsI WCGNNNNNNNCGW 1 cut(s) 84
PctI GAATGC 1 cut(s) 155
PdmI GAANNNNTTC 1 cut(s) 480
PfeI GAWTC 1 cut(s) 266
PflFI GACNNNGTC 1 cut(s) 7
PkrI GCNGC 3 cut(s) 112, 416, 597
PleI GAGTC 4 cut(s) 14, 322, 580, 697
PpsI GAGTC 4 cut(s) 14, 322, 580, 697
PshBI ATTAAT 1 cut(s) 429
Psp124BI GAGCTC 1 cut(s) 408
Psp6I CCWGG 1 cut(s) 194
PspGI CCWGG 1 cut(s) 194
PspPI GGNCC 1 cut(s) 800
PsyI GACNNNGTC 1 cut(s) 7
SacI GAGCTC 1 cut(s) 408
SacII CCGCGG 1 cut(s) 375
SapI GCTCTTC 1 cut(s) 105
SaqAI TTAA 2 cut(s) 429, 495
SatI GCNGC 3 cut(s) 111, 415, 596
Sau3AI GATC 2 cut(s) 75, 170
Sau96I GGNCC 1 cut(s) 800
SchI GAGTC 4 cut(s) 14, 323, 580, 697
ScrFI CCNGG 2 cut(s) 196, 304
SduI GDGCHC 2 cut(s) 60, 408
Sfr274I CTCGAG 1 cut(s) 588
Sfr303I CCGCGG 1 cut(s) 375
SgrBI CCGCGG 1 cut(s) 375
SlaI CTCGAG 1 cut(s) 588
SmlI CTYRAG 1 cut(s) 588
SmoI CTYRAG 1 cut(s) 588
Sse9I AATT 6 cut(s) 69, 426, 524, 544, 566, 572
SsiI CCGC 6 cut(s) 111, 123, 328, 372, 374, 414
SspMI CTAG 1 cut(s) 288
SstI GAGCTC 1 cut(s) 408
StyD4I CCNGG 2 cut(s) 194, 302
TaaI ACNGT 1 cut(s) 148
TaqI TCGA 5 cut(s) 105, 169, 282, 471, 589
TasI AATT 6 cut(s) 69, 426, 524, 544, 566, 572
TauI GCSGC 2 cut(s) 113, 417
TfiI GAWTC 1 cut(s) 266
Tru1I TTAA 2 cut(s) 429, 495
Tru9I TTAA 2 cut(s) 429, 495
TscAI CASTG 1 cut(s) 153
TseFI GTSAC 1 cut(s) 355
TseI GCWGC 1 cut(s) 595
Tsp45I GTSAC 1 cut(s) 355
TspDTI ATGAA 3 cut(s) 153, 455, 768
TspRI CASTG 1 cut(s) 153
Tth111I GACNNNGTC 1 cut(s) 7
VspI ATTAAT 1 cut(s) 429
XagI CCTNNNNNAGG 1 cut(s) 603
XapI RAATTY 1 cut(s) 524
XhoI CTCGAG 1 cut(s) 588
XmnI GAANNNNTTC 1 cut(s) 480
XspI CTAG 1 cut(s) 288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.