Rw5G037220

exonuclease V

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0002756
Physical Location & Seq
Forward (+)
65539753 .. 65543486
3734 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G037220.1

Sequence Viewer

Length: 1197 bp
ATGACCCGGTCGCCTTCCGACTCACTCCTCAATCATCCTCCTCGTCCTCATCTTGTTCCTGAGATCCCAATTGAGATCGTCAGCGACGAAGAGATGGCTCTGCTCGAAGCCGCGTTGCTCTCCGCTGGCTCTCTGATCCCTTCCGTGAGAGCATTCCCTTCATTTCGATCCTCTCACTTCCCTTCCAATGTCAGGTCTATTCAGTCCATCACCCTCGTTTCCAAGAAGAGATTTTCGGGTTGCTCCGAACCGATATTGAGGATTCGGGTTTCAGGGTTCGACACTAGCCAGAAGAAGACCCGGTTGGCTGAGTCGTTTTTGCACCGCTTCAGGAGGAAGAAGGGCTTGTGCGTCACCGATTTTACTTCCGCGGAATGGTGTGAAAAACAGATGGAGTTTGAGCTCCTCGGCGGCAAGAAAAAAATTAATAAGGCTATGAAAGCTGGTAGTAAGCGCCATGCAAAACTCGAAGAAGAGGTTCTAACAAGAGTTAAAGTTGATATCAAATCAGACGAAGATAAATTTGCTCTGAAGCTTTTGAATTGTATTACCGGCATAAATCAATTACAATTTGAAGGATTGACTCGAGAGCTGCCTCTCATAGGTTTTGTAGAAGGTGTATGGATGGTGGGAGTGATTGATGAGGTTCAAATGCCTGCAACAGAAACCCAGAGAAATCCATTATTAGTTGACACAAAGACTCGTGTGAAAGATACACTTCCTGCTGAACCACAACGAAGGAATGGAAGGCTTCAATTGATGTGCTACAAGTATATGTGGGACAATTTAGTTACTTCTGAATTCCCCTCCGACCAATTCTTTGATTATTTTTCCTTAAATCCTGATTGTGACTTATCTGAAGAAATCAGGGAGGTCACAGCTAAATCAGGGTGTCCAGCAAAGACCCTTGATGACGTAGTGAGATACTATAGAAATACCTGGAGCATGCTACCCCCTGCTCATGACCAACTTTTATTGAGATACGAGTTACAAAAAGACCATTCTCTAATCGGCGAAGATAAATTTGCATACGACTCTGATTGGGTTGAGAAGCAAATTCAGTTTTCTCTCGAGTTCTGGTTAGGAGAGCGAGAAGCCAGTTACACTCCTGAGGAAGAGCGATGGAAATGCAACTTCTGTCAATTTGCTTCCACATGTCCTGCTAAAAGTGTAACCTCCAGCAACCTGCTGACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo5

Protein Analysis

398

Amino Acids

45.65

Weight (kDa)

6.44

Isoelectric Point (pI)

48.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo5 PF09810 105 - 222 1.2e-36 Exonuclease V - a 5' deoxyribonuclease
PDDEXK_1 PF12705 192 - 387 3.3e-06 PD-(D/E)XK nuclease superfamily
Exo5 PF09810 222 - 290 4.6e-08 Exonuclease V - a 5' deoxyribonuclease
Exo5 PF09810 320 - 386 1.8e-11 Exonuclease V - a 5' deoxyribonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 113, 371
AciI CCGC 6 cut(s) 111, 123, 325, 369, 371, 411
AclWI GGATC 3 cut(s) 58, 130, 162
AcsI RAATTY 4 cut(s) 521, 800, 1022, 1056
AcuI CTGAAG 3 cut(s) 313, 551, 879
AfiI CCNNNNNNNGG 3 cut(s) 192, 375, 602
AflIII ACRYGT 1 cut(s) 1154
AgsI TTSAA 4 cut(s) 541, 575, 650, 755
AjnI CCWGG 1 cut(s) 938
AjuI GAANNNNNNNTTGG 2 cut(s) 287, 319
AluBI AGCT 5 cut(s) 403, 443, 535, 592, 881
AluI AGCT 5 cut(s) 403, 443, 535, 592, 881
Alw21I GWGCWC 1 cut(s) 405
AlwI GGATC 3 cut(s) 58, 130, 162
Ama87I CYCGRG 2 cut(s) 585, 1070
ApeKI GCWGC 1 cut(s) 592
ApoI RAATTY 4 cut(s) 521, 800, 1022, 1056
ArsI GACNNNNNNTTYG 2 cut(s) 803, 835
AseI ATTAAT 1 cut(s) 426
Asp700I GAANNNNTTC 1 cut(s) 477
AspLEI GCGC 1 cut(s) 456
AsuC2I CCSGG 2 cut(s) 7, 301
AsuHPI GGTGA 2 cut(s) 202, 346
AvaI CYCGRG 2 cut(s) 585, 1070
AxyI CCTNAGG 1 cut(s) 1110
BanII GRGCYC 1 cut(s) 405
BauI CACGAG 1 cut(s) 702
BbsI GAAGAC 1 cut(s) 302
Bbv12I GWGCWC 1 cut(s) 405
BbvI GCAGC 1 cut(s) 579
BccI CCATC 5 cut(s) 88, 215, 385, 619, 1116
BcgI CGANNNNNNTGC 2 cut(s) 1110, 1144
BciT130I CCWGG 1 cut(s) 940
BcnI CCSGG 2 cut(s) 7, 301
BfaI CTAG 1 cut(s) 285
BfmI CTRYAG 1 cut(s) 928
BfoI RGCGCY 1 cut(s) 457
BisI GCNGC 3 cut(s) 111, 412, 593
BlsI GCNGC 3 cut(s) 112, 413, 594
Bme1390I CCNGG 3 cut(s) 7, 301, 940
BmeT110I CYCGRG 2 cut(s) 585, 1070
BmrFI CCNGG 3 cut(s) 7, 301, 940
BpiI GAAGAC 1 cut(s) 302
BpmI CTGGAG 2 cut(s) 961, 1162
BpuMI CCSGG 2 cut(s) 7, 301
BsaJI CCNNGG 2 cut(s) 369, 406
Bsc4I CCNNNNNNNGG 3 cut(s) 192, 375, 602
Bse118I RCCGGY 1 cut(s) 551
Bse1I ACTGG 1 cut(s) 1098
Bse21I CCTNAGG 1 cut(s) 1110
BseBI CCWGG 1 cut(s) 940
BseDI CCNNGG 2 cut(s) 369, 406
BseGI GGATG 2 cut(s) 34, 630
BseLI CCNNNNNNNGG 3 cut(s) 192, 375, 602
BseMII CTCAG 3 cut(s) 51, 300, 1101
BseNI ACTGG 1 cut(s) 1098
BseRI GAGGAG 3 cut(s) 17, 30, 395
BseXI GCAGC 1 cut(s) 579
Bsh1236I CGCG 2 cut(s) 113, 371
Bsh1285I CGRYCG 1 cut(s) 11
BsiEI CGRYCG 1 cut(s) 11
BsiHKAI GWGCWC 1 cut(s) 405
BsiHKCI CYCGRG 2 cut(s) 585, 1070
BsiSI CCGG 3 cut(s) 7, 301, 552
BslFI GGGAC 1 cut(s) 794
BslI CCNNNNNNNGG 3 cut(s) 192, 375, 602
BsmFI GGGAC 1 cut(s) 794
BsmI GAATGC 1 cut(s) 152
BsoBI CYCGRG 2 cut(s) 585, 1070
Bsp1286I GDGCHC 1 cut(s) 405
Bsp143I GATC 4 cut(s) 63, 75, 135, 167
BspACI CCGC 6 cut(s) 111, 123, 325, 369, 371, 411
BspCNI CTCAG 3 cut(s) 52, 301, 1102
BspFNI CGCG 2 cut(s) 113, 371
BspHI TCATGA 1 cut(s) 961
BspPI GGATC 3 cut(s) 58, 130, 162
BspQI GCTCTTC 1 cut(s) 1110
BsrFI RCCGGY 1 cut(s) 551
BsrI ACTGG 1 cut(s) 1098
BssAI RCCGGY 1 cut(s) 551
BssECI CCNNGG 2 cut(s) 369, 406
BssMI GATC 4 cut(s) 63, 75, 135, 167
BssSI CACGAG 1 cut(s) 702
Bst2BI CACGAG 1 cut(s) 702
Bst2UI CCWGG 1 cut(s) 940
Bst6I CTCTTC 4 cut(s) 84, 221, 468, 1110
BstC8I GCNNGC 3 cut(s) 127, 657, 947
BstDEI CTNAG 4 cut(s) 60, 309, 1110, 1194
BstDSI CCRYGG 1 cut(s) 369
BstENI CCTNNNNNAGG 1 cut(s) 600
BstF5I GGATG 2 cut(s) 34, 630
BstFNI CGCG 2 cut(s) 113, 371
BstH2I RGCGCY 1 cut(s) 457
BstHHI GCGC 1 cut(s) 456
BstKTI GATC 4 cut(s) 66, 78, 138, 170
BstMBI GATC 4 cut(s) 63, 75, 135, 167
BstMCI CGRYCG 1 cut(s) 11
BstMWI GCNNNNNNNGC 1 cut(s) 440
BstNI CCWGG 1 cut(s) 940
BstNSI RCATGY 2 cut(s) 949, 1158
BstSCI CCNGG 3 cut(s) 5, 299, 938
BstSFI CTRYAG 1 cut(s) 928
BstUI CGCG 2 cut(s) 113, 371
BstV1I GCAGC 1 cut(s) 579
BstV2I GAAGAC 1 cut(s) 302
BstX2I RGATCY 1 cut(s) 63
BstYI RGATCY 1 cut(s) 63
Bsu36I CCTNAGG 1 cut(s) 1110
BtgI CCRYGG 1 cut(s) 369
BtgZI GCGATG 1 cut(s) 1135
BtsCI GGATG 2 cut(s) 34, 630
Cac8I GCNNGC 3 cut(s) 127, 657, 947
CciI TCATGA 1 cut(s) 961
CfoI GCGC 1 cut(s) 456
Cfr10I RCCGGY 1 cut(s) 551
Cfr42I CCGCGG 1 cut(s) 372
CseI GACGC 1 cut(s) 340
CviAII CATG 4 cut(s) 458, 946, 962, 1155
DdeI CTNAG 4 cut(s) 60, 309, 1110, 1194
DpnI GATC 4 cut(s) 65, 77, 137, 169
DpnII GATC 4 cut(s) 63, 75, 135, 167
Eam1104I CTCTTC 4 cut(s) 84, 221, 468, 1110
EarI CTCTTC 4 cut(s) 84, 221, 468, 1110
Ecl136II GAGCTC 1 cut(s) 403
Eco24I GRGCYC 1 cut(s) 405
Eco32I GATATC 1 cut(s) 502
Eco53kI GAGCTC 1 cut(s) 403
Eco57I CTGAAG 3 cut(s) 313, 551, 879
Eco81I CCTNAGG 1 cut(s) 1110
Eco88I CYCGRG 2 cut(s) 585, 1070
EcoICRI GAGCTC 1 cut(s) 403
EcoNI CCTNNNNNAGG 1 cut(s) 600
EcoRI GAATTC 1 cut(s) 800
EcoRII CCWGG 1 cut(s) 938
EcoRV GATATC 1 cut(s) 502
EcoT38I GRGCYC 1 cut(s) 405
FaeI CATG 4 cut(s) 461, 949, 965, 1158
FalI AAGNNNNNCTT 4 cut(s) 329, 361, 702, 734
FaqI GGGAC 1 cut(s) 794
FatI CATG 4 cut(s) 457, 945, 961, 1154
Fnu4HI GCNGC 3 cut(s) 111, 412, 593
FokI GGATG 2 cut(s) 21, 637
FriOI GRGCYC 1 cut(s) 405
Fsp4HI GCNGC 3 cut(s) 111, 412, 593
FspBI CTAG 1 cut(s) 285
GlaI GCGC 1 cut(s) 455
GluI GCNGC 3 cut(s) 111, 412, 593
GsuI CTGGAG 2 cut(s) 961, 1162
HaeII RGCGCY 1 cut(s) 457
HapII CCGG 3 cut(s) 7, 301, 552
HgaI GACGC 1 cut(s) 340
HhaI GCGC 1 cut(s) 456
Hin1II CATG 4 cut(s) 461, 949, 965, 1158
Hin6I GCGC 1 cut(s) 454
HinP1I GCGC 1 cut(s) 454
HincII GTYRAC 1 cut(s) 691
HindII GTYRAC 1 cut(s) 691
HindIII AAGCTT 1 cut(s) 533
HinfI GANTC 6 cut(s) 20, 262, 311, 583, 700, 1034
HpaII CCGG 3 cut(s) 7, 301, 552
HphI GGTGA 2 cut(s) 202, 346
Hpy166II GTNNAC 1 cut(s) 691
Hpy188I TCNGA 9 cut(s) 19, 135, 247, 511, 531, 799, 811, 859, 1039
Hpy188III TCNNGA 7 cut(s) 59, 331, 587, 842, 962, 1070, 1109
Hpy8I GTNNAC 1 cut(s) 691
Hpy99I CGWCG 1 cut(s) 89
HpyAV CCTTC 9 cut(s) 24, 150, 168, 192, 334, 569, 608, 732, 741
HpyCH4IV ACGT 1 cut(s) 915
HpyCH4V TGCA 5 cut(s) 322, 461, 659, 1028, 1131
HpyF10VI GCNNNNNNNGC 1 cut(s) 440
HpyF3I CTNAG 4 cut(s) 60, 309, 1110, 1194
HpySE526I ACGT 1 cut(s) 915
Hsp92II CATG 4 cut(s) 461, 949, 965, 1158
HspAI GCGC 1 cut(s) 454
KspI CCGCGG 1 cut(s) 372
Kzo9I GATC 4 cut(s) 63, 75, 135, 167
LguI GCTCTTC 1 cut(s) 1110
LmnI GCTCC 3 cut(s) 248, 408, 942
Lsp1109I GCAGC 1 cut(s) 579
MaeI CTAG 1 cut(s) 285
MaeII ACGT 1 cut(s) 915
MaeIII GTNAC 7 cut(s) 352, 790, 848, 874, 987, 1100, 1171
MalI GATC 4 cut(s) 65, 77, 137, 169
MboI GATC 4 cut(s) 63, 75, 135, 167
MfeI CAATTG 2 cut(s) 69, 755
MflI RGATCY 1 cut(s) 63
MhlI GDGCHC 1 cut(s) 405
MlyI GAGTC 5 cut(s) 14, 320, 577, 694, 1028
MmeI TCCRAC 2 cut(s) 42, 834
MroXI GAANNNNTTC 1 cut(s) 477
MseI TTAA 3 cut(s) 426, 492, 836
MspA1I CMGCKG 2 cut(s) 125, 371
MspI CCGG 3 cut(s) 7, 301, 552
MspR9I CCNGG 3 cut(s) 7, 301, 940
MunI CAATTG 2 cut(s) 69, 755
Mva1269I GAATGC 1 cut(s) 152
MvaI CCWGG 1 cut(s) 940
MvnI CGCG 2 cut(s) 113, 371
MwoI GCNNNNNNNGC 1 cut(s) 440
NciI CCSGG 2 cut(s) 7, 301
NdeII GATC 4 cut(s) 63, 75, 135, 167
NlaIII CATG 4 cut(s) 461, 949, 965, 1158
NmeAIII GCCGAG 1 cut(s) 387
NmuCI GTSAC 3 cut(s) 352, 848, 874
NspI RCATGY 2 cut(s) 949, 1158
PaeI GCATGC 1 cut(s) 949
PaeR7I CTCGAG 2 cut(s) 585, 1070
PagI TCATGA 1 cut(s) 961
PciI ACATGT 1 cut(s) 1154
PciSI GCTCTTC 1 cut(s) 1110
PcsI WCGNNNNNNNCGW 1 cut(s) 84
PctI GAATGC 1 cut(s) 152
PdmI GAANNNNTTC 1 cut(s) 477
PfeI GAWTC 1 cut(s) 262
PflFI GACNNNGTC 1 cut(s) 7
PkrI GCNGC 3 cut(s) 112, 413, 594
PleI GAGTC 5 cut(s) 14, 319, 577, 694, 1028
PpsI GAGTC 5 cut(s) 14, 319, 577, 694, 1028
PscI ACATGT 1 cut(s) 1154
PshBI ATTAAT 1 cut(s) 426
Psp124BI GAGCTC 1 cut(s) 405
Psp6I CCWGG 1 cut(s) 938
PspGI CCWGG 1 cut(s) 938
PsuI RGATCY 1 cut(s) 63
PsyI GACNNNGTC 1 cut(s) 7
SacI GAGCTC 1 cut(s) 405
SacII CCGCGG 1 cut(s) 372
SapI GCTCTTC 1 cut(s) 1110
SaqAI TTAA 3 cut(s) 426, 492, 836
SatI GCNGC 3 cut(s) 111, 412, 593
Sau3AI GATC 4 cut(s) 63, 75, 135, 167
SchI GAGTC 5 cut(s) 14, 320, 577, 694, 1028
ScrFI CCNGG 3 cut(s) 7, 301, 940
SduI GDGCHC 1 cut(s) 405
SfcI CTRYAG 1 cut(s) 928
Sfr274I CTCGAG 2 cut(s) 585, 1070
Sfr303I CCGCGG 1 cut(s) 372
SgrBI CCGCGG 1 cut(s) 372
SlaI CTCGAG 2 cut(s) 585, 1070
SmlI CTYRAG 2 cut(s) 585, 1070
SmoI CTYRAG 2 cut(s) 585, 1070
SphI GCATGC 1 cut(s) 949
SsiI CCGC 6 cut(s) 111, 123, 325, 369, 371, 411
SspMI CTAG 1 cut(s) 285
SstI GAGCTC 1 cut(s) 405
StyD4I CCNGG 3 cut(s) 5, 299, 938
TaiI ACGT 1 cut(s) 918
TaqI TCGA 6 cut(s) 105, 166, 279, 468, 586, 1071
TauI GCSGC 2 cut(s) 113, 414
TfiI GAWTC 1 cut(s) 262
Tru1I TTAA 3 cut(s) 426, 492, 836
Tru9I TTAA 3 cut(s) 426, 492, 836
TseFI GTSAC 3 cut(s) 352, 848, 874
TseI GCWGC 1 cut(s) 592
Tsp45I GTSAC 3 cut(s) 352, 848, 874
TspDTI ATGAA 2 cut(s) 150, 452
TspGWI ACGGA 1 cut(s) 133
Tth111I GACNNNGTC 1 cut(s) 7
VspI ATTAAT 1 cut(s) 426
XagI CCTNNNNNAGG 1 cut(s) 600
XapI RAATTY 4 cut(s) 521, 800, 1022, 1056
XceI RCATGY 2 cut(s) 949, 1158
XhoI CTCGAG 2 cut(s) 585, 1070
XmnI GAANNNNTTC 1 cut(s) 477
XspI CTAG 1 cut(s) 285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.