MD02G1279000.v1.1

exonuclease V

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0002756
Physical Location & Seq
Reverse (-)
33509146 .. 33511385
2240 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1279000.v1.1.491

Sequence Viewer

Length: 333 bp
ATGGCTGCTAACTCAAGGTTCCCAGCAGAGACCCTTGATGACATAGTGAGATACTACAGAAACACATGTAGCATGCTGCCCTCTACACATGACCAGCTGCTATTGAGATATGAGTTACAGAAAGATCATTCGTTACTTGGTGAAGATGAGTTTGCATACGACTCTGATTGGGTCAAGAATCAAATACAGGGTTGTCTTGAGTTCTGGTTAAGAGAGCGAGAAACAAGTTACACTCCTAAGGATGAGGGATGGAAATGCAGGTTCTGTCAATATTCTTCTGTTTTCCCCGCTAGACGTGCAAAGTCTGCAAACTATACTAATACACCAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo5

Protein Analysis

111

Amino Acids

13.0

Weight (kDa)

5.65

Isoelectric Point (pI)

37.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo5 PF09810 25 - 93 1.9e-09 Exonuclease V - a 5' deoxyribonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 249
AciI CCGC 1 cut(s) 288
AflIII ACRYGT 1 cut(s) 65
AjiI CACGTC 1 cut(s) 296
AluBI AGCT 1 cut(s) 97
AluI AGCT 1 cut(s) 97
Alw26I GTCTC 1 cut(s) 23
AlwNI CAGNNNCTG 1 cut(s) 264
ApeKI GCWGC 3 cut(s) 5, 76, 97
AsuHPI GGTGA 1 cut(s) 152
AxyI CCTNAGG 1 cut(s) 237
BbvI GCAGC 2 cut(s) 63, 84
BccI CCATC 1 cut(s) 243
BcoDI GTCTC 1 cut(s) 23
BfaI CTAG 1 cut(s) 291
BfmI CTRYAG 1 cut(s) 55
BfuAI ACCTGC 1 cut(s) 249
BisI GCNGC 3 cut(s) 6, 77, 98
BlsI GCNGC 3 cut(s) 7, 78, 99
BmgBI CACGTC 1 cut(s) 296
BmiI GGNNCC 1 cut(s) 20
BpuEI CTTGAG 1 cut(s) 218
BsaI GGTCTC 1 cut(s) 23
Bse21I CCTNAGG 1 cut(s) 237
BseGI GGATG 2 cut(s) 247, 254
BseXI GCAGC 2 cut(s) 63, 84
BseYI CCCAGC 1 cut(s) 22
BsmAI GTCTC 1 cut(s) 23
Bso31I GGTCTC 1 cut(s) 23
Bsp143I GATC 1 cut(s) 124
BspACI CCGC 1 cut(s) 288
BspLI GGNNCC 1 cut(s) 20
BspMI ACCTGC 1 cut(s) 249
BspTNI GGTCTC 1 cut(s) 23
BssMI GATC 1 cut(s) 124
BstAPI GCANNNNNTGC 1 cut(s) 305
BstC8I GCNNGC 1 cut(s) 74
BstDEI CTNAG 1 cut(s) 237
BstF5I GGATG 2 cut(s) 247, 254
BstKTI GATC 1 cut(s) 127
BstMAI GTCTC 1 cut(s) 23
BstMBI GATC 1 cut(s) 124
BstMWI GCNNNNNNNGC 2 cut(s) 296, 305
BstNSI RCATGY 2 cut(s) 69, 76
BstSFI CTRYAG 1 cut(s) 55
BstV1I GCAGC 2 cut(s) 63, 84
Bsu36I CCTNAGG 1 cut(s) 237
BtrI CACGTC 1 cut(s) 296
BtsCI GGATG 2 cut(s) 247, 254
BveI ACCTGC 1 cut(s) 249
Cac8I GCNNGC 1 cut(s) 74
CaiI CAGNNNCTG 1 cut(s) 264
CviAII CATG 3 cut(s) 66, 73, 89
CviJI RGCY 2 cut(s) 5, 97
CviKI_1 RGCY 2 cut(s) 5, 97
DdeI CTNAG 1 cut(s) 237
DpnI GATC 1 cut(s) 126
DpnII GATC 1 cut(s) 124
Eco31I GGTCTC 1 cut(s) 23
Eco81I CCTNAGG 1 cut(s) 237
FaeI CATG 3 cut(s) 69, 76, 92
FaiI YATR 7 cut(s) 44, 67, 74, 90, 111, 157, 315
FatI CATG 3 cut(s) 65, 72, 88
FauI CCCGC 1 cut(s) 295
Fnu4HI GCNGC 3 cut(s) 6, 77, 98
FokI GGATG 2 cut(s) 254, 261
Fsp4HI GCNGC 3 cut(s) 6, 77, 98
FspBI CTAG 1 cut(s) 291
GluI GCNGC 3 cut(s) 6, 77, 98
GsaI CCCAGC 1 cut(s) 26
Hin1II CATG 3 cut(s) 69, 76, 92
HinfI GANTC 2 cut(s) 161, 178
HphI GGTGA 1 cut(s) 152
Hpy188I TCNGA 1 cut(s) 166
Hpy188III TCNNGA 2 cut(s) 175, 197
HpyCH4IV ACGT 1 cut(s) 295
HpyCH4V TGCA 4 cut(s) 155, 258, 299, 308
HpyF10VI GCNNNNNNNGC 2 cut(s) 296, 305
HpyF3I CTNAG 1 cut(s) 237
HpySE526I ACGT 1 cut(s) 295
Hsp92II CATG 3 cut(s) 69, 76, 92
Kzo9I GATC 1 cut(s) 124
LpnPI CCDG 5 cut(s) 36, 107, 173, 190, 244
Lsp1109I GCAGC 2 cut(s) 63, 84
MaeI CTAG 1 cut(s) 291
MaeII ACGT 1 cut(s) 295
MaeIII GTNAC 3 cut(s) 114, 132, 227
MalI GATC 1 cut(s) 126
MboI GATC 1 cut(s) 124
MboII GAAGA 2 cut(s) 155, 267
MlyI GAGTC 1 cut(s) 155
MnlI CCTC 2 cut(s) 91, 238
MseI TTAA 1 cut(s) 209
MspA1I CMGCKG 1 cut(s) 97
MwoI GCNNNNNNNGC 2 cut(s) 296, 305
NdeII GATC 1 cut(s) 124
NlaIII CATG 3 cut(s) 69, 76, 92
NlaIV GGNNCC 1 cut(s) 20
NspI RCATGY 2 cut(s) 69, 76
PaeI GCATGC 1 cut(s) 76
PciI ACATGT 1 cut(s) 65
PfeI GAWTC 1 cut(s) 178
PkrI GCNGC 3 cut(s) 7, 78, 99
PleI GAGTC 1 cut(s) 155
PpsI GAGTC 1 cut(s) 155
PscI ACATGT 1 cut(s) 65
PspFI CCCAGC 1 cut(s) 22
PspN4I GGNNCC 1 cut(s) 20
PstNI CAGNNNCTG 1 cut(s) 264
PvuII CAGCTG 1 cut(s) 97
SaqAI TTAA 1 cut(s) 209
SatI GCNGC 3 cut(s) 6, 77, 98
Sau3AI GATC 1 cut(s) 124
SchI GAGTC 1 cut(s) 155
SetI ASST 4 cut(s) 20, 99, 263, 298
SfcI CTRYAG 1 cut(s) 55
SmlI CTYRAG 2 cut(s) 13, 197
SmoI CTYRAG 2 cut(s) 13, 197
SphI GCATGC 1 cut(s) 76
SsiI CCGC 1 cut(s) 288
SspI AATATT 1 cut(s) 272
SspMI CTAG 1 cut(s) 291
TaiI ACGT 1 cut(s) 298
TfiI GAWTC 1 cut(s) 178
Tru1I TTAA 1 cut(s) 209
Tru9I TTAA 1 cut(s) 209
TseI GCWGC 3 cut(s) 5, 76, 97
XceI RCATGY 2 cut(s) 69, 76
XspI CTAG 1 cut(s) 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.