FvH4_2g19080

Cyclin-dependent kinase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0002441
Physical Location & Seq
Reverse (-)
16269530 .. 16272935
3406 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g19080.t1

Sequence Viewer

Length: 705 bp
ATGGGAAAATACATGAAGAAATCCAAAATTACCGGCGACATCGCAGTCATGGAGGTTTCAATTTCACCTCAATCCTCTCTCGGCGTCCGCACCCGAGCCAGAACCCTAGCCCTTCAGAAGCTCCAGCAGCAGCAACTGCCTCAGCTACAGCCACAGCAATCGCCCGACTCCGATTCGTCCTCGCTCTACTATCTCCAGCTCCGGAGCCGCCGCCTCGAGAAGCCGCCTTTGCGAAGCGACGGCAAGAAGCCGGGCCGGCCGGTGGCGACTTCGCGGCCCAGTTCGAGGCTGAGTGTGGGATCGTCGGCGGATTTGGGGTGGGATGAGAGGAAGGAGACGGAGGGGTGTTTTGGTAATATCGGATGTGAAGCTGACGATGTTGAGGCTTCTTTTGGGGAAAACAACTTGGATTTTGAAACCAGAGAGAGAAGCACCCGAGAAAGCACGCCATGCAGCTTGATAAGGAATTCGGACACTATTGGAACCCCCAGTTCTACAACAAGGCGGTCAAGTTCTATTGCAAATAACCAAAGAGCTAGAAATGATACACAAAGAACAATTCCAACTGCACTTGATATGGACGAGTTCTTTGCACAAGCTGAGCGGCAGCAACAGAAACTGTTTATGGAGAAGTACAACTTTGACATTACCATTGATATTCCCCTCCCAGGAAGGTATGAATGGGAGCAAGTGATTCCCTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CDI

Protein Analysis

235

Amino Acids

26.42

Weight (kDa)

8.41

Isoelectric Point (pI)

61.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDI PF02234 186 - 231 1.3e-16 Cyclin-dependent kinase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 44
AccBSI CCGCTC 1 cut(s) 604
AccII CGCG 1 cut(s) 274
AccIII TCCGGA 1 cut(s) 201
AciI CCGC 8 cut(s) 88, 208, 211, 224, 274, 308, 505, 604
AclWI GGATC 1 cut(s) 307
AcoI YGGCCR 1 cut(s) 257
AcsI RAATTY 1 cut(s) 466
AcuI CTGAAG 1 cut(s) 98
AcyI GRCGYC 1 cut(s) 84
AfaI GTAC 1 cut(s) 635
AfiI CCNNNNNNNGG 3 cut(s) 262, 285, 668
AgsI TTSAA 2 cut(s) 60, 416
AjnI CCWGG 1 cut(s) 667
AluBI AGCT 7 cut(s) 121, 145, 199, 371, 456, 536, 599
AluI AGCT 7 cut(s) 121, 145, 199, 371, 456, 536, 599
Alw26I GTCTC 1 cut(s) 329
AlwI GGATC 1 cut(s) 307
AlwNI CAGNNNCTG 2 cut(s) 136, 619
Ama87I CYCGRG 3 cut(s) 93, 215, 435
Aor13HI TCCGGA 1 cut(s) 201
AoxI GGCC 3 cut(s) 253, 257, 275
ApeKI GCWGC 4 cut(s) 127, 130, 453, 607
ApoI RAATTY 1 cut(s) 466
ArsI GACNNNNNNTTYG 2 cut(s) 572, 604
AspS9I GGNCC 2 cut(s) 253, 276
AsuC2I CCSGG 1 cut(s) 252
AsuHPI GGTGA 1 cut(s) 57
AvaI CYCGRG 3 cut(s) 93, 215, 435
BbvCI CCTCAGC 1 cut(s) 141
BbvI GCAGC 4 cut(s) 139, 142, 465, 619
BceAI ACGGC 1 cut(s) 256
BcgI CGANNNNNNTGC 2 cut(s) 572, 606
BciT130I CCWGG 1 cut(s) 669
BcnI CCSGG 1 cut(s) 252
BcoDI GTCTC 1 cut(s) 329
BfaI CTAG 2 cut(s) 107, 537
BfmI CTRYAG 1 cut(s) 146
BglI GCCNNNNNGGC 1 cut(s) 256
BisI GCNGC 9 cut(s) 128, 131, 208, 211, 224, 275, 454, 605, 608
BlpI GCTNAGC 1 cut(s) 600
BlsI GCNGC 9 cut(s) 129, 132, 209, 212, 225, 276, 455, 606, 609
Bme1390I CCNGG 2 cut(s) 252, 669
BmeT110I CYCGRG 3 cut(s) 93, 215, 435
BmgT120I GGNCC 2 cut(s) 253, 276
BmiI GGNNCC 2 cut(s) 206, 484
BmrFI CCNGG 2 cut(s) 252, 669
BmrI ACTGGG 2 cut(s) 273, 483
BmuI ACTGGG 2 cut(s) 273, 483
BpmI CTGGAG 2 cut(s) 107, 179
Bpu10I CCTNAGC 1 cut(s) 141
Bpu1102I GCTNAGC 1 cut(s) 600
BpuMI CCSGG 1 cut(s) 252
BsaHI GRCGYC 1 cut(s) 84
BsaJI CCNNGG 1 cut(s) 667
BsaWI WCCGGW 1 cut(s) 201
Bsc4I CCNNNNNNNGG 3 cut(s) 262, 285, 668
Bse118I RCCGGY 3 cut(s) 32, 255, 259
Bse1I ACTGG 2 cut(s) 279, 489
BseAI TCCGGA 1 cut(s) 201
BseBI CCWGG 1 cut(s) 669
BseDI CCNNGG 1 cut(s) 667
BseGI GGATG 2 cut(s) 328, 368
BseLI CCNNNNNNNGG 3 cut(s) 262, 285, 668
BseMII CTCAG 3 cut(s) 155, 281, 591
BseNI ACTGG 2 cut(s) 279, 489
BseX3I CGGCCG 1 cut(s) 257
BseXI GCAGC 4 cut(s) 139, 142, 465, 619
BsgI GTGCAG 1 cut(s) 552
Bsh1236I CGCG 1 cut(s) 274
Bsh1285I CGRYCG 1 cut(s) 260
BshFI GGCC 3 cut(s) 255, 259, 277
BsiEI CGRYCG 1 cut(s) 260
BsiHKCI CYCGRG 3 cut(s) 93, 215, 435
BsiSI CCGG 5 cut(s) 33, 202, 251, 256, 260
BslI CCNNNNNNNGG 3 cut(s) 262, 285, 668
BsmAI GTCTC 1 cut(s) 329
BsmBI CGTCTC 1 cut(s) 329
BsnI GGCC 3 cut(s) 255, 259, 277
BsoBI CYCGRG 3 cut(s) 93, 215, 435
Bsp13I TCCGGA 1 cut(s) 201
Bsp143I GATC 1 cut(s) 299
Bsp1720I GCTNAGC 1 cut(s) 600
BspACI CCGC 8 cut(s) 88, 208, 211, 224, 274, 308, 505, 604
BspANI GGCC 3 cut(s) 255, 259, 277
BspCNI CTCAG 3 cut(s) 154, 282, 592
BspEI TCCGGA 1 cut(s) 201
BspFNI CGCG 1 cut(s) 274
BspLI GGNNCC 2 cut(s) 206, 484
BspPI GGATC 1 cut(s) 307
BsrBI CCGCTC 1 cut(s) 604
BsrFI RCCGGY 3 cut(s) 32, 255, 259
BsrI ACTGG 2 cut(s) 279, 489
BssAI RCCGGY 3 cut(s) 32, 255, 259
BssECI CCNNGG 1 cut(s) 667
BssMI GATC 1 cut(s) 299
BssNI GRCGYC 1 cut(s) 84
Bst2UI CCWGG 1 cut(s) 669
Bst4CI ACNGT 1 cut(s) 621
BstACI GRCGYC 1 cut(s) 84
BstAPI GCANNNNNTGC 2 cut(s) 136, 450
BstC8I GCNNGC 2 cut(s) 257, 446
BstDEI CTNAG 3 cut(s) 141, 290, 600
BstF5I GGATG 2 cut(s) 328, 368
BstFNI CGCG 1 cut(s) 274
BstKTI GATC 1 cut(s) 302
BstMAI GTCTC 1 cut(s) 329
BstMBI GATC 1 cut(s) 299
BstMCI CGRYCG 1 cut(s) 260
BstMWI GCNNNNNNNGC 5 cut(s) 127, 136, 229, 256, 450
BstNI CCWGG 1 cut(s) 669
BstSCI CCNGG 2 cut(s) 250, 667
BstSFI CTRYAG 1 cut(s) 146
BstUI CGCG 1 cut(s) 274
BstV1I GCAGC 4 cut(s) 139, 142, 465, 619
BstZI CGGCCG 1 cut(s) 257
BsuRI GGCC 3 cut(s) 255, 259, 277
BtgZI GCGATG 1 cut(s) 25
BtsCI GGATG 2 cut(s) 328, 368
Cac8I GCNNGC 2 cut(s) 257, 446
CaiI CAGNNNCTG 2 cut(s) 136, 619
Cfr10I RCCGGY 3 cut(s) 32, 255, 259
Cfr13I GGNCC 2 cut(s) 253, 276
CseI GACGC 1 cut(s) 73
Csp6I GTAC 1 cut(s) 634
CviAII CATG 3 cut(s) 13, 49, 450
CviQI GTAC 1 cut(s) 634
DdeI CTNAG 3 cut(s) 141, 290, 600
DpnI GATC 1 cut(s) 301
DpnII GATC 1 cut(s) 299
DrdI GACNNNNNNGTC 1 cut(s) 44
DseDI GACNNNNNNGTC 1 cut(s) 44
EaeI YGGCCR 1 cut(s) 257
EagI CGGCCG 1 cut(s) 257
EciI GGCGGA 1 cut(s) 323
EclXI CGGCCG 1 cut(s) 257
Eco52I CGGCCG 1 cut(s) 257
Eco57I CTGAAG 1 cut(s) 98
Eco88I CYCGRG 3 cut(s) 93, 215, 435
EcoRI GAATTC 1 cut(s) 466
EcoRII CCWGG 1 cut(s) 667
Esp3I CGTCTC 1 cut(s) 329
FaeI CATG 3 cut(s) 16, 52, 453
FaiI YATR 6 cut(s) 14, 50, 451, 578, 626, 678
FalI AAGNNNNNCTT 2 cut(s) 623, 655
FatI CATG 3 cut(s) 12, 48, 449
Fnu4HI GCNGC 9 cut(s) 128, 131, 208, 211, 224, 275, 454, 605, 608
FokI GGATG 2 cut(s) 335, 375
FseI GGCCGGCC 1 cut(s) 259
Fsp4HI GCNGC 9 cut(s) 128, 131, 208, 211, 224, 275, 454, 605, 608
FspBI CTAG 2 cut(s) 107, 537
GluI GCNGC 9 cut(s) 128, 131, 208, 211, 224, 275, 454, 605, 608
GsuI CTGGAG 2 cut(s) 107, 179
HaeIII GGCC 3 cut(s) 255, 259, 277
HapII CCGG 5 cut(s) 33, 202, 251, 256, 260
HgaI GACGC 1 cut(s) 73
Hin1I GRCGYC 1 cut(s) 84
Hin1II CATG 3 cut(s) 16, 52, 453
HinfI GANTC 3 cut(s) 167, 173, 694
HpaII CCGG 5 cut(s) 33, 202, 251, 256, 260
HphI GGTGA 1 cut(s) 57
Hpy188I TCNGA 4 cut(s) 117, 172, 362, 472
Hpy188III TCNNGA 2 cut(s) 202, 217
Hpy99I CGWCG 2 cut(s) 242, 307
HpyAV CCTTC 3 cut(s) 122, 325, 666
HpyCH4III ACNGT 1 cut(s) 621
HpyCH4V TGCA 4 cut(s) 453, 521, 569, 593
HpyF10VI GCNNNNNNNGC 5 cut(s) 127, 136, 229, 256, 450
HpyF3I CTNAG 3 cut(s) 141, 290, 600
Hsp92I GRCGYC 1 cut(s) 84
Hsp92II CATG 3 cut(s) 16, 52, 453
Kpn2I TCCGGA 1 cut(s) 201
KroI GCCGGC 1 cut(s) 255
KroNI GCCGGC 1 cut(s) 257
Kzo9I GATC 1 cut(s) 299
LmnI GCTCC 4 cut(s) 126, 204, 204, 685
Lsp1109I GCAGC 4 cut(s) 139, 142, 465, 619
MaeI CTAG 2 cut(s) 107, 537
MalI GATC 1 cut(s) 301
MbiI CCGCTC 1 cut(s) 604
MboI GATC 1 cut(s) 299
MboII GAAGA 1 cut(s) 28
MluCI AATT 4 cut(s) 27, 60, 466, 558
MlyI GAGTC 1 cut(s) 161
MmeI TCCRAC 1 cut(s) 587
MroI TCCGGA 1 cut(s) 201
MroNI GCCGGC 1 cut(s) 255
MspI CCGG 5 cut(s) 33, 202, 251, 256, 260
MspR9I CCNGG 2 cut(s) 252, 669
MvaI CCWGG 1 cut(s) 669
MvnI CGCG 1 cut(s) 274
MwoI GCNNNNNNNGC 5 cut(s) 127, 136, 229, 256, 450
NaeI GCCGGC 1 cut(s) 257
NciI CCSGG 1 cut(s) 252
NdeII GATC 1 cut(s) 299
NgoMIV GCCGGC 1 cut(s) 255
NlaIII CATG 3 cut(s) 16, 52, 453
NlaIV GGNNCC 2 cut(s) 206, 484
NmeAIII GCCGAG 1 cut(s) 60
PaeR7I CTCGAG 1 cut(s) 215
PdiI GCCGGC 1 cut(s) 257
PfeI GAWTC 2 cut(s) 173, 694
PkrI GCNGC 9 cut(s) 129, 132, 209, 212, 225, 276, 455, 606, 609
PleI GAGTC 1 cut(s) 161
PpsI GAGTC 1 cut(s) 161
Psp6I CCWGG 1 cut(s) 667
PspGI CCWGG 1 cut(s) 667
PspN4I GGNNCC 2 cut(s) 206, 484
PspPI GGNCC 2 cut(s) 253, 276
PstNI CAGNNNCTG 2 cut(s) 136, 619
RigI GGCCGGCC 1 cut(s) 259
RsaI GTAC 1 cut(s) 635
RsaNI GTAC 1 cut(s) 634
SatI GCNGC 9 cut(s) 128, 131, 208, 211, 224, 275, 454, 605, 608
Sau3AI GATC 1 cut(s) 299
Sau96I GGNCC 2 cut(s) 253, 276
SchI GAGTC 1 cut(s) 161
ScrFI CCNGG 2 cut(s) 252, 669
SfcI CTRYAG 1 cut(s) 146
Sfr274I CTCGAG 1 cut(s) 215
SlaI CTCGAG 1 cut(s) 215
SmlI CTYRAG 1 cut(s) 215
SmoI CTYRAG 1 cut(s) 215
Sse9I AATT 4 cut(s) 27, 60, 466, 558
SsiI CCGC 8 cut(s) 88, 208, 211, 224, 274, 308, 505, 604
SspMI CTAG 2 cut(s) 107, 537
StyD4I CCNGG 2 cut(s) 250, 667
TaaI ACNGT 1 cut(s) 621
TaqI TCGA 2 cut(s) 216, 284
TasI AATT 4 cut(s) 27, 60, 466, 558
TatI WGTACW 1 cut(s) 633
TauI GCSGC 5 cut(s) 210, 213, 226, 277, 607
TfiI GAWTC 2 cut(s) 173, 694
TseI GCWGC 4 cut(s) 127, 130, 453, 607
TspDTI ATGAA 2 cut(s) 29, 693
TspGWI ACGGA 1 cut(s) 353
XapI RAATTY 1 cut(s) 466
XhoI CTCGAG 1 cut(s) 215
XspI CTAG 2 cut(s) 107, 537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.