RchiOBHm_Chr6g0284901

Cyclin-dependent kinase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0002441
Physical Location & Seq
Reverse (-)
48198920 .. 48202040
3121 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ25552

Sequence Viewer

Length: 720 bp
ATGGGAAAATACATGAAGAAATCCAAGATCACAGGCGAAATCGCCGTCATGGAGGTTTCAATTTCACCTCAGTCCTCCCTCGGCGTTCGAACCAGAGCCAAAACCCTAGCCCTCCAGAAGCTCCACCACCAGCAGCAGCAATCGCCCGACTCCGATTCCTCGTCTCTGTACTATCTCCAGCTCCGAAGCCGCCGCCTTGAGAAGCCGCCGCTGCGAAGCGACGCCAACAAGCAGGCTCAGGCTCAGGGTCCTCGGGATTGCTACGCGGGGGGCGGGGCCGTCAATTCGAGGTTGAGTGTGGGCTCGTCGGCGGATTTGGGCTGGGATGAGAGGAACGAGAAGGACGGGGAGGGGTGTTTTGGTAATGTTGAAAGTGAAGCAGAGTATGCCAACGATGTTGAGGCTTCATTTGGGGAAAACAACTTGGATTTTGATGGCAGAGATAGGAGCACCCGAGAAAGCACGCCATGCAGCTTGATAAGGAACTCAGACTCTGTTGGAACCCCTGGTTCTACAACAAGGCGGTCAAGTTCTATTGCAAATAACCAAAGAGTTAGAAATGATACCCAAAGAACCATTCCAACTGCACTTGATATGGACGAGTTCTTTGCCCAAGCTGAGCAGCAGCAACAGAAACTCTTTATGGAGAAGTACAACTTTGATATTACCAGTGATATGCCCCTTCCAGGAAGGTATGAATGGGAGCAAGTGATTCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CDI

Protein Analysis

239

Amino Acids

26.67

Weight (kDa)

5.4

Isoelectric Point (pI)

56.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDI PF02234 192 - 237 3.3e-18 Cyclin-dependent kinase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 266
AciI CCGC 8 cut(s) 190, 193, 206, 209, 266, 273, 311, 523
AcyI GRCGYC 1 cut(s) 222
AfaI GTAC 2 cut(s) 170, 653
AfiI CCNNNNNNNGG 1 cut(s) 686
AgsI TTSAA 2 cut(s) 60, 371
AjnI CCWGG 2 cut(s) 505, 685
AjuI GAANNNNNNNTTGG 2 cut(s) 561, 593
AluBI AGCT 4 cut(s) 121, 181, 474, 617
AluI AGCT 4 cut(s) 121, 181, 474, 617
Alw21I GWGCWC 1 cut(s) 452
Alw26I GTCTC 1 cut(s) 168
AlwNI CAGNNNCTG 1 cut(s) 494
Ama87I CYCGRG 2 cut(s) 252, 453
AoxI GGCC 1 cut(s) 276
ApeKI GCWGC 6 cut(s) 133, 136, 211, 471, 622, 625
ArsI GACNNNNNNTTYG 4 cut(s) 30, 62, 590, 622
AspS9I GGNCC 2 cut(s) 248, 276
AsuHPI GGTGA 1 cut(s) 57
AsuII TTCGAA 1 cut(s) 88
AvaI CYCGRG 2 cut(s) 252, 453
AvaII GGWCC 1 cut(s) 248
BanII GRGCYC 1 cut(s) 305
Bbv12I GWGCWC 1 cut(s) 452
BbvI GCAGC 6 cut(s) 145, 148, 198, 483, 634, 637
BccI CCATC 1 cut(s) 428
BceAI ACGGC 2 cut(s) 29, 263
BcgI CGANNNNNNTGC 2 cut(s) 590, 624
BciT130I CCWGG 2 cut(s) 507, 687
BcoDI GTCTC 1 cut(s) 168
BfaI CTAG 1 cut(s) 107
BlpI GCTNAGC 1 cut(s) 618
Bme1390I CCNGG 2 cut(s) 507, 687
Bme18I GGWCC 1 cut(s) 248
BmeT110I CYCGRG 2 cut(s) 252, 453
BmgT120I GGNCC 2 cut(s) 248, 276
BmiI GGNNCC 3 cut(s) 249, 277, 502
BmrFI CCNGG 2 cut(s) 507, 687
BpmI CTGGAG 2 cut(s) 98, 161
Bpu10I CCTNAGC 2 cut(s) 237, 243
Bpu1102I GCTNAGC 1 cut(s) 618
Bpu14I TTCGAA 1 cut(s) 88
BpuEI CTTGAG 1 cut(s) 218
BsaHI GRCGYC 1 cut(s) 222
BsaJI CCNNGG 3 cut(s) 79, 251, 505
Bsc4I CCNNNNNNNGG 1 cut(s) 686
Bse1I ACTGG 1 cut(s) 669
BseBI CCWGG 2 cut(s) 507, 687
BseDI CCNNGG 3 cut(s) 79, 251, 505
BseGI GGATG 1 cut(s) 331
BseLI CCNNNNNNNGG 1 cut(s) 686
BseMII CTCAG 5 cut(s) 83, 251, 257, 501, 609
BseNI ACTGG 1 cut(s) 669
BseXI GCAGC 6 cut(s) 145, 148, 198, 483, 634, 637
BseYI CCCAGC 1 cut(s) 321
BsgI GTGCAG 1 cut(s) 570
Bsh1236I CGCG 1 cut(s) 266
BshFI GGCC 1 cut(s) 278
BsiHKAI GWGCWC 1 cut(s) 452
BsiHKCI CYCGRG 2 cut(s) 252, 453
BslI CCNNNNNNNGG 1 cut(s) 686
BsmAI GTCTC 1 cut(s) 168
BsmBI CGTCTC 1 cut(s) 168
BsnI GGCC 1 cut(s) 278
BsoBI CYCGRG 2 cut(s) 252, 453
Bsp119I TTCGAA 1 cut(s) 88
Bsp1286I GDGCHC 2 cut(s) 305, 452
Bsp143I GATC 1 cut(s) 27
Bsp1720I GCTNAGC 1 cut(s) 618
BspACI CCGC 8 cut(s) 190, 193, 206, 209, 266, 273, 311, 523
BspANI GGCC 1 cut(s) 278
BspCNI CTCAG 5 cut(s) 82, 250, 256, 500, 610
BspFNI CGCG 1 cut(s) 266
BspLI GGNNCC 3 cut(s) 249, 277, 502
BspT104I TTCGAA 1 cut(s) 88
BsrI ACTGG 1 cut(s) 669
BssECI CCNNGG 3 cut(s) 79, 251, 505
BssMI GATC 1 cut(s) 27
BssNI GRCGYC 1 cut(s) 222
Bst2UI CCWGG 2 cut(s) 507, 687
BstACI GRCGYC 1 cut(s) 222
BstAPI GCANNNNNTGC 2 cut(s) 386, 468
BstBI TTCGAA 1 cut(s) 88
BstC8I GCNNGC 2 cut(s) 234, 464
BstDEI CTNAG 5 cut(s) 69, 237, 243, 487, 618
BstF5I GGATG 1 cut(s) 331
BstFNI CGCG 1 cut(s) 266
BstKTI GATC 1 cut(s) 30
BstMAI GTCTC 1 cut(s) 168
BstMBI GATC 1 cut(s) 27
BstMWI GCNNNNNNNGC 4 cut(s) 142, 211, 386, 468
BstNI CCWGG 2 cut(s) 507, 687
BstSCI CCNGG 2 cut(s) 505, 685
BstUI CGCG 1 cut(s) 266
BstV1I GCAGC 6 cut(s) 145, 148, 198, 483, 634, 637
BsuRI GGCC 1 cut(s) 278
BtsCI GGATG 1 cut(s) 331
BtsIMutI CAGTG 1 cut(s) 676
Cac8I GCNNGC 2 cut(s) 234, 464
CaiI CAGNNNCTG 1 cut(s) 494
Cfr13I GGNCC 2 cut(s) 248, 276
CseI GACGC 1 cut(s) 230
Csp6I GTAC 2 cut(s) 169, 652
CviAII CATG 3 cut(s) 13, 49, 468
CviQI GTAC 2 cut(s) 169, 652
DdeI CTNAG 5 cut(s) 69, 237, 243, 487, 618
DpnI GATC 1 cut(s) 29
DpnII GATC 1 cut(s) 27
EciI GGCGGA 1 cut(s) 326
Eco24I GRGCYC 1 cut(s) 305
Eco47I GGWCC 1 cut(s) 248
Eco88I CYCGRG 2 cut(s) 252, 453
EcoO109I RGGNCCY 1 cut(s) 248
EcoRII CCWGG 2 cut(s) 505, 685
EcoT38I GRGCYC 1 cut(s) 305
Esp3I CGTCTC 1 cut(s) 168
FaeI CATG 3 cut(s) 16, 52, 471
FaiI YATR 8 cut(s) 14, 50, 387, 469, 596, 644, 677, 696
FalI AAGNNNNNCTT 2 cut(s) 641, 673
FatI CATG 3 cut(s) 12, 48, 467
FauI CCCGC 2 cut(s) 259, 266
FokI GGATG 1 cut(s) 338
FriOI GRGCYC 1 cut(s) 305
FspBI CTAG 1 cut(s) 107
GsaI CCCAGC 1 cut(s) 325
GsuI CTGGAG 2 cut(s) 98, 161
HaeIII GGCC 1 cut(s) 278
HgaI GACGC 1 cut(s) 230
Hin1I GRCGYC 1 cut(s) 222
Hin1II CATG 3 cut(s) 16, 52, 471
HinfI GANTC 4 cut(s) 149, 155, 491, 712
HphI GGTGA 1 cut(s) 57
Hpy188I TCNGA 3 cut(s) 154, 185, 490
Hpy188III TCNNGA 2 cut(s) 115, 254
Hpy99I CGWCG 2 cut(s) 224, 310
HpyAV CCTTC 3 cut(s) 334, 684, 692
HpyCH4V TGCA 3 cut(s) 471, 539, 587
HpyF10VI GCNNNNNNNGC 4 cut(s) 142, 211, 386, 468
HpyF3I CTNAG 5 cut(s) 69, 237, 243, 487, 618
Hsp92I GRCGYC 1 cut(s) 222
Hsp92II CATG 3 cut(s) 16, 52, 471
Kzo9I GATC 1 cut(s) 27
LmnI GCTCC 4 cut(s) 126, 186, 447, 703
Lsp1109I GCAGC 6 cut(s) 145, 148, 198, 483, 634, 637
MaeI CTAG 1 cut(s) 107
MalI GATC 1 cut(s) 29
MboI GATC 1 cut(s) 27
MboII GAAGA 1 cut(s) 28
MhlI GDGCHC 2 cut(s) 305, 452
MluCI AATT 2 cut(s) 60, 283
MlyI GAGTC 2 cut(s) 143, 485
MmeI TCCRAC 2 cut(s) 478, 605
MspA1I CMGCKG 1 cut(s) 211
MspR9I CCNGG 2 cut(s) 507, 687
MvaI CCWGG 2 cut(s) 507, 687
MvnI CGCG 1 cut(s) 266
MwoI GCNNNNNNNGC 4 cut(s) 142, 211, 386, 468
NdeII GATC 1 cut(s) 27
NlaIII CATG 3 cut(s) 16, 52, 471
NlaIV GGNNCC 3 cut(s) 249, 277, 502
NmeAIII GCCGAG 1 cut(s) 60
NspV TTCGAA 1 cut(s) 88
PfeI GAWTC 2 cut(s) 155, 712
PfoI TCCNGGA 1 cut(s) 685
PleI GAGTC 2 cut(s) 143, 485
PpsI GAGTC 2 cut(s) 143, 485
PpuMI RGGWCCY 1 cut(s) 248
Psp5II RGGWCCY 1 cut(s) 248
Psp6I CCWGG 2 cut(s) 505, 685
PspFI CCCAGC 1 cut(s) 321
PspGI CCWGG 2 cut(s) 505, 685
PspN4I GGNNCC 3 cut(s) 249, 277, 502
PspPI GGNCC 2 cut(s) 248, 276
PspPPI RGGWCCY 1 cut(s) 248
PstNI CAGNNNCTG 1 cut(s) 494
RsaI GTAC 2 cut(s) 170, 653
RsaNI GTAC 2 cut(s) 169, 652
Sau3AI GATC 1 cut(s) 27
Sau96I GGNCC 2 cut(s) 248, 276
SchI GAGTC 2 cut(s) 143, 485
ScrFI CCNGG 2 cut(s) 507, 687
SduI GDGCHC 2 cut(s) 305, 452
SetI ASST 8 cut(s) 57, 70, 123, 183, 293, 476, 619, 695
SfuI TTCGAA 1 cut(s) 88
SinI GGWCC 1 cut(s) 248
SmlI CTYRAG 1 cut(s) 197
SmoI CTYRAG 1 cut(s) 197
Sse9I AATT 2 cut(s) 60, 283
SsiI CCGC 8 cut(s) 190, 193, 206, 209, 266, 273, 311, 523
SspMI CTAG 1 cut(s) 107
StyD4I CCNGG 2 cut(s) 505, 685
TaqI TCGA 2 cut(s) 88, 287
TasI AATT 2 cut(s) 60, 283
TatI WGTACW 2 cut(s) 168, 651
TauI GCSGC 4 cut(s) 192, 195, 208, 211
TfiI GAWTC 2 cut(s) 155, 712
TscAI CASTG 1 cut(s) 676
TseI GCWGC 6 cut(s) 133, 136, 211, 471, 622, 625
TspDTI ATGAA 3 cut(s) 29, 396, 711
TspRI CASTG 1 cut(s) 676
VpaK11BI GGWCC 1 cut(s) 248
XspI CTAG 1 cut(s) 107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.