MD10G1164700.v1.1

Cyclin-dependent kinase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0002441
Physical Location & Seq
Reverse (-)
25541334 .. 25543495
2162 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1164700.v1.1.491

Sequence Viewer

Length: 351 bp
ATGGTAACGATTTGGGGGTCGAGGCTTCGTTTGGGGAAAACAACTTGGATTTTGAAGCCAGAGACAGCACTGGTTTTGAGCACTAGAGAAACCACGCCGTGCAGCTTCATAAGGGAGTCGGACACAATTGGAACCCCCGGTTCCACCACAAGGCGGACGAGTTCTATTTCAACTCACCGGAGAGTTAGAAACAATGTGCAAAGAAATATCCCAACCACGCTTGAGATGGAGGAGTTCTTTGCCCGACATGAGGAGGAGCAACAGAAAATCTTCATTGAGAAGTACAACTTTGACATCATCAACGATTTACCCCTTCCTGGAAGGTATGAATGGGAGCAAGTGATTCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CDI

Protein Analysis

117

Amino Acids

13.61

Weight (kDa)

7.93

Isoelectric Point (pI)

45.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDI PF02234 68 - 114 8.1e-20 Cyclin-dependent kinase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 154
AdeI CACNNNGTG 1 cut(s) 99
AfaI GTAC 1 cut(s) 284
AfiI CCNNNNNNNGG 4 cut(s) 150, 153, 250, 317
AgsI TTSAA 2 cut(s) 55, 171
AjnI CCWGG 1 cut(s) 316
AluBI AGCT 1 cut(s) 105
AluI AGCT 1 cut(s) 105
Alw21I GWGCWC 1 cut(s) 83
Alw26I GTCTC 1 cut(s) 56
ApeKI GCWGC 1 cut(s) 102
Asp700I GAANNNNTTC 1 cut(s) 269
AsuC2I CCSGG 1 cut(s) 138
AsuHPI GGTGA 1 cut(s) 167
Bbv12I GWGCWC 1 cut(s) 83
BbvI GCAGC 1 cut(s) 114
BccI CCATC 1 cut(s) 220
BceAI ACGGC 1 cut(s) 82
BciT130I CCWGG 1 cut(s) 318
BcnI CCSGG 1 cut(s) 138
BcoDI GTCTC 1 cut(s) 56
BfaI CTAG 1 cut(s) 84
BisI GCNGC 1 cut(s) 103
BlsI GCNGC 1 cut(s) 104
Bme1390I CCNGG 2 cut(s) 138, 318
BmiI GGNNCC 2 cut(s) 133, 142
BmrFI CCNGG 2 cut(s) 138, 318
BpuEI CTTGAG 1 cut(s) 242
BpuMI CCSGG 1 cut(s) 138
BsaJI CCNNGG 1 cut(s) 136
BsaWI WCCGGW 1 cut(s) 177
Bsc4I CCNNNNNNNGG 4 cut(s) 150, 153, 250, 317
Bse1I ACTGG 1 cut(s) 75
BseBI CCWGG 1 cut(s) 318
BseDI CCNNGG 1 cut(s) 136
BseLI CCNNNNNNNGG 4 cut(s) 150, 153, 250, 317
BseNI ACTGG 1 cut(s) 75
BseRI GAGGAG 3 cut(s) 245, 266, 269
BseXI GCAGC 1 cut(s) 114
BsgI GTGCAG 1 cut(s) 121
BsiHKAI GWGCWC 1 cut(s) 83
BsiSI CCGG 2 cut(s) 138, 178
BslI CCNNNNNNNGG 4 cut(s) 150, 153, 250, 317
BsmAI GTCTC 1 cut(s) 56
Bsp1286I GDGCHC 1 cut(s) 83
BspACI CCGC 1 cut(s) 154
BspLI GGNNCC 2 cut(s) 133, 142
BsrI ACTGG 1 cut(s) 75
BssECI CCNNGG 1 cut(s) 136
Bst2UI CCWGG 1 cut(s) 318
BstMAI GTCTC 1 cut(s) 56
BstNI CCWGG 1 cut(s) 318
BstSCI CCNGG 2 cut(s) 136, 316
BstV1I GCAGC 1 cut(s) 114
BtsIMutI CAGTG 1 cut(s) 68
Csp6I GTAC 1 cut(s) 283
CviAII CATG 1 cut(s) 248
CviJI RGCY 3 cut(s) 25, 58, 105
CviKI_1 RGCY 3 cut(s) 25, 58, 105
CviQI GTAC 1 cut(s) 283
DraIII CACNNNGTG 1 cut(s) 99
EciI GGCGGA 1 cut(s) 169
EcoRII CCWGG 1 cut(s) 316
FaeI CATG 1 cut(s) 251
FaiI YATR 3 cut(s) 110, 249, 327
FalI AAGNNNNNCTT 2 cut(s) 272, 304
FatI CATG 1 cut(s) 247
Fnu4HI GCNGC 1 cut(s) 103
Fsp4HI GCNGC 1 cut(s) 103
FspBI CTAG 1 cut(s) 84
GluI GCNGC 1 cut(s) 103
HapII CCGG 2 cut(s) 138, 178
Hin1II CATG 1 cut(s) 251
HinfI GANTC 2 cut(s) 116, 343
HpaII CCGG 2 cut(s) 138, 178
HphI GGTGA 1 cut(s) 167
Hpy188I TCNGA 1 cut(s) 121
HpyAV CCTTC 2 cut(s) 315, 323
HpyCH4V TGCA 2 cut(s) 102, 199
Hsp92II CATG 1 cut(s) 251
LmnI GCTCC 2 cut(s) 256, 334
LpnPI CCDG 6 cut(s) 56, 72, 151, 191, 303, 330
Lsp1109I GCAGC 1 cut(s) 114
MaeI CTAG 1 cut(s) 84
MaeIII GTNAC 1 cut(s) 4
MboII GAAGA 1 cut(s) 262
MfeI CAATTG 1 cut(s) 126
MhlI GDGCHC 1 cut(s) 83
MluCI AATT 1 cut(s) 126
MlyI GAGTC 1 cut(s) 125
MmeI TCCRAC 1 cut(s) 99
MnlI CCTC 4 cut(s) 15, 223, 244, 247
MroXI GAANNNNTTC 1 cut(s) 269
MspI CCGG 2 cut(s) 138, 178
MspR9I CCNGG 2 cut(s) 138, 318
MunI CAATTG 1 cut(s) 126
MvaI CCWGG 1 cut(s) 318
NciI CCSGG 1 cut(s) 138
NlaIII CATG 1 cut(s) 251
NlaIV GGNNCC 2 cut(s) 133, 142
PdmI GAANNNNTTC 1 cut(s) 269
PfeI GAWTC 1 cut(s) 343
PfoI TCCNGGA 1 cut(s) 316
PkrI GCNGC 1 cut(s) 104
PleI GAGTC 1 cut(s) 124
PpsI GAGTC 1 cut(s) 124
Psp6I CCWGG 1 cut(s) 316
PspGI CCWGG 1 cut(s) 316
PspN4I GGNNCC 2 cut(s) 133, 142
RsaI GTAC 1 cut(s) 284
RsaNI GTAC 1 cut(s) 283
SatI GCNGC 1 cut(s) 103
SchI GAGTC 1 cut(s) 125
ScrFI CCNGG 2 cut(s) 138, 318
SduI GDGCHC 1 cut(s) 83
SetI ASST 2 cut(s) 107, 326
SmlI CTYRAG 1 cut(s) 221
SmoI CTYRAG 1 cut(s) 221
Sse9I AATT 1 cut(s) 126
SsiI CCGC 1 cut(s) 154
SspMI CTAG 1 cut(s) 84
StyD4I CCNGG 2 cut(s) 136, 316
TaqI TCGA 1 cut(s) 20
TasI AATT 1 cut(s) 126
TatI WGTACW 1 cut(s) 282
TfiI GAWTC 1 cut(s) 343
TscAI CASTG 1 cut(s) 75
TseI GCWGC 1 cut(s) 102
TspDTI ATGAA 3 cut(s) 97, 262, 342
TspRI CASTG 1 cut(s) 75
XcmI CCANNNNNNNNNTGG 1 cut(s) 223
XmnI GAANNNNTTC 1 cut(s) 269
XspI CTAG 1 cut(s) 84
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.