Rroxscaffold_7G00183970

Cyclin-dependent kinase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0002441
Physical Location & Seq
Forward (+)
23146162 .. 23149154
2993 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00183970.1

Sequence Viewer

Length: 738 bp
ATGGGAAAATACATGAAGAAATCCAAAATCACAGGCGACATCGCCGTCATGGAGGTTTCAATTTCACCTCAGTCCTCCCTCGGCGTTCGAACCAGAGCCAAAACCCTAGCCCTCCAGAAGCTCCACCAGCAGCCGCAGCAGCAATCGCCCGACTCCGATTCCTCGTCTCTGTACTATCTCCAGCTCCGAAGCCGCCGCCTTGAGAAGCCGCCGCTTCGAAGCGACGCCAACAAGCACGCTCAGGCTCAGGCTCAGGCTCAGGGTCCTCGGGATTGCTGCGCGGGAGTGAACTCGAGGCCCAATTCGAGGTTGAGTGTGGGCTCGTCGGCGGATTTGGGCTGGGATGAGAGGAAGGAGAAGGACGGGGAGGGGTGTTTTGGTAATGTTGAAAGTGAAGCAGAGTATGCCAACGATGTTGAGGCTTCATTTGGGGAAAACAACTTGGATTTTGATGGCAGAGATAGGAGCACCCGAGAAAGCACGCCATGCAGCTTGATAAGGAACTCTGACTCTGTTGGAACCCCTGGTTCTACAACCAGGCGGTCAAGTTCTATTTCAAATAACCAAAGAGTTAGAAATGATACCCAAAGAACCATTCCAACTGCACTTGATATGGACGAGTTCTTTGCCCAAGCTGAGCAACAGCAACAGAAACTCTTTATGGAGAAGTACAACTTTGATATTACCAGTGATATGCCCCTTCCAGGAAGGTATGAATGGGAGCAAGTGATTCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CDI

Protein Analysis

245

Amino Acids

27.39

Weight (kDa)

5.84

Isoelectric Point (pI)

55.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDI PF02234 198 - 243 3.4e-18 Cyclin-dependent kinase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 44
AccII CGCG 1 cut(s) 281
AciI CCGC 8 cut(s) 134, 193, 196, 209, 212, 281, 329, 541
AcyI GRCGYC 1 cut(s) 225
AfaI GTAC 2 cut(s) 173, 671
AfiI CCNNNNNNNGG 2 cut(s) 306, 704
AgsI TTSAA 3 cut(s) 60, 389, 558
AjnI CCWGG 3 cut(s) 523, 536, 703
AjuI GAANNNNNNNTTGG 2 cut(s) 579, 611
AluBI AGCT 4 cut(s) 121, 184, 492, 635
AluI AGCT 4 cut(s) 121, 184, 492, 635
Alw21I GWGCWC 1 cut(s) 470
Alw26I GTCTC 1 cut(s) 171
Ama87I CYCGRG 3 cut(s) 267, 292, 471
AoxI GGCC 1 cut(s) 296
ApeKI GCWGC 5 cut(s) 130, 136, 139, 276, 489
ArsI GACNNNNNNTTYG 2 cut(s) 608, 640
AspLEI GCGC 1 cut(s) 281
AspS9I GGNCC 2 cut(s) 263, 297
AsuHPI GGTGA 1 cut(s) 57
AsuII TTCGAA 2 cut(s) 88, 217
AvaI CYCGRG 3 cut(s) 267, 292, 471
AvaII GGWCC 1 cut(s) 263
BanII GRGCYC 1 cut(s) 323
Bbv12I GWGCWC 1 cut(s) 470
BbvI GCAGC 5 cut(s) 142, 148, 151, 263, 501
BccI CCATC 1 cut(s) 446
BceAI ACGGC 1 cut(s) 29
BcgI CGANNNNNNTGC 2 cut(s) 608, 642
BciT130I CCWGG 3 cut(s) 525, 538, 705
BcoDI GTCTC 1 cut(s) 171
BfaI CTAG 1 cut(s) 107
BlpI GCTNAGC 1 cut(s) 636
Bme1390I CCNGG 3 cut(s) 525, 538, 705
Bme18I GGWCC 1 cut(s) 263
BmeT110I CYCGRG 3 cut(s) 267, 292, 471
BmgT120I GGNCC 2 cut(s) 263, 297
BmiI GGNNCC 2 cut(s) 264, 520
BmrFI CCNGG 3 cut(s) 525, 538, 705
BpmI CTGGAG 2 cut(s) 98, 164
Bpu10I CCTNAGC 4 cut(s) 240, 246, 252, 258
Bpu1102I GCTNAGC 1 cut(s) 636
Bpu14I TTCGAA 2 cut(s) 88, 217
BpuEI CTTGAG 1 cut(s) 221
BsaHI GRCGYC 1 cut(s) 225
BsaJI CCNNGG 3 cut(s) 79, 266, 523
Bsc4I CCNNNNNNNGG 2 cut(s) 306, 704
Bse1I ACTGG 1 cut(s) 687
BseBI CCWGG 3 cut(s) 525, 538, 705
BseDI CCNNGG 3 cut(s) 79, 266, 523
BseGI GGATG 1 cut(s) 349
BseLI CCNNNNNNNGG 2 cut(s) 306, 704
BseMII CTCAG 6 cut(s) 83, 254, 260, 266, 272, 627
BseNI ACTGG 1 cut(s) 687
BseXI GCAGC 5 cut(s) 142, 148, 151, 263, 501
BseYI CCCAGC 1 cut(s) 339
BsgI GTGCAG 1 cut(s) 588
Bsh1236I CGCG 1 cut(s) 281
BshFI GGCC 1 cut(s) 298
BsiHKAI GWGCWC 1 cut(s) 470
BsiHKCI CYCGRG 3 cut(s) 267, 292, 471
BslI CCNNNNNNNGG 2 cut(s) 306, 704
BsmAI GTCTC 1 cut(s) 171
BsmBI CGTCTC 1 cut(s) 171
BsnI GGCC 1 cut(s) 298
BsoBI CYCGRG 3 cut(s) 267, 292, 471
Bsp119I TTCGAA 2 cut(s) 88, 217
Bsp1286I GDGCHC 2 cut(s) 323, 470
Bsp1720I GCTNAGC 1 cut(s) 636
BspACI CCGC 8 cut(s) 134, 193, 196, 209, 212, 281, 329, 541
BspANI GGCC 1 cut(s) 298
BspCNI CTCAG 6 cut(s) 82, 253, 259, 265, 271, 628
BspFNI CGCG 1 cut(s) 281
BspLI GGNNCC 2 cut(s) 264, 520
BspT104I TTCGAA 2 cut(s) 88, 217
BsrI ACTGG 1 cut(s) 687
BssECI CCNNGG 3 cut(s) 79, 266, 523
BssNI GRCGYC 1 cut(s) 225
Bst2UI CCWGG 3 cut(s) 525, 538, 705
BstACI GRCGYC 1 cut(s) 225
BstAPI GCANNNNNTGC 2 cut(s) 404, 486
BstBI TTCGAA 2 cut(s) 88, 217
BstC8I GCNNGC 2 cut(s) 237, 482
BstDEI CTNAG 6 cut(s) 69, 240, 246, 252, 258, 636
BstF5I GGATG 1 cut(s) 349
BstFNI CGCG 1 cut(s) 281
BstHHI GCGC 1 cut(s) 281
BstMAI GTCTC 1 cut(s) 171
BstMWI GCNNNNNNNGC 6 cut(s) 127, 136, 139, 145, 404, 486
BstNI CCWGG 3 cut(s) 525, 538, 705
BstSCI CCNGG 3 cut(s) 523, 536, 703
BstUI CGCG 1 cut(s) 281
BstV1I GCAGC 5 cut(s) 142, 148, 151, 263, 501
BsuRI GGCC 1 cut(s) 298
BtgZI GCGATG 1 cut(s) 25
BtsCI GGATG 1 cut(s) 349
BtsIMutI CAGTG 1 cut(s) 694
Cac8I GCNNGC 2 cut(s) 237, 482
CfoI GCGC 1 cut(s) 281
Cfr13I GGNCC 2 cut(s) 263, 297
CseI GACGC 1 cut(s) 233
Csp6I GTAC 2 cut(s) 172, 670
CviAII CATG 3 cut(s) 13, 49, 486
CviQI GTAC 2 cut(s) 172, 670
DdeI CTNAG 6 cut(s) 69, 240, 246, 252, 258, 636
DrdI GACNNNNNNGTC 1 cut(s) 44
DseDI GACNNNNNNGTC 1 cut(s) 44
EciI GGCGGA 1 cut(s) 344
Eco24I GRGCYC 1 cut(s) 323
Eco47I GGWCC 1 cut(s) 263
Eco88I CYCGRG 3 cut(s) 267, 292, 471
EcoO109I RGGNCCY 1 cut(s) 263
EcoRII CCWGG 3 cut(s) 523, 536, 703
EcoT38I GRGCYC 1 cut(s) 323
Esp3I CGTCTC 1 cut(s) 171
FaeI CATG 3 cut(s) 16, 52, 489
FaiI YATR 8 cut(s) 14, 50, 405, 487, 614, 662, 695, 714
FalI AAGNNNNNCTT 2 cut(s) 659, 691
FatI CATG 3 cut(s) 12, 48, 485
FauI CCCGC 1 cut(s) 274
FokI GGATG 1 cut(s) 356
FriOI GRGCYC 1 cut(s) 323
FspBI CTAG 1 cut(s) 107
GlaI GCGC 1 cut(s) 280
GsaI CCCAGC 1 cut(s) 343
GsuI CTGGAG 2 cut(s) 98, 164
HaeIII GGCC 1 cut(s) 298
HgaI GACGC 1 cut(s) 233
HhaI GCGC 1 cut(s) 281
Hin1I GRCGYC 1 cut(s) 225
Hin1II CATG 3 cut(s) 16, 52, 489
Hin6I GCGC 1 cut(s) 279
HinP1I GCGC 1 cut(s) 279
HinfI GANTC 4 cut(s) 152, 158, 509, 730
HphI GGTGA 1 cut(s) 57
Hpy166II GTNNAC 1 cut(s) 289
Hpy188I TCNGA 3 cut(s) 157, 188, 508
Hpy188III TCNNGA 2 cut(s) 115, 269
Hpy8I GTNNAC 1 cut(s) 289
Hpy99I CGWCG 2 cut(s) 227, 328
HpyAV CCTTC 4 cut(s) 346, 352, 702, 710
HpyCH4V TGCA 2 cut(s) 489, 605
HpyF10VI GCNNNNNNNGC 6 cut(s) 127, 136, 139, 145, 404, 486
HpyF3I CTNAG 6 cut(s) 69, 240, 246, 252, 258, 636
Hsp92I GRCGYC 1 cut(s) 225
Hsp92II CATG 3 cut(s) 16, 52, 489
HspAI GCGC 1 cut(s) 279
LmnI GCTCC 4 cut(s) 126, 189, 465, 721
Lsp1109I GCAGC 5 cut(s) 142, 148, 151, 263, 501
MaeI CTAG 1 cut(s) 107
MboII GAAGA 1 cut(s) 28
MhlI GDGCHC 2 cut(s) 323, 470
MluCI AATT 2 cut(s) 60, 301
MlyI GAGTC 2 cut(s) 146, 503
MmeI TCCRAC 2 cut(s) 496, 623
MspR9I CCNGG 3 cut(s) 525, 538, 705
MvaI CCWGG 3 cut(s) 525, 538, 705
MvnI CGCG 1 cut(s) 281
MwoI GCNNNNNNNGC 6 cut(s) 127, 136, 139, 145, 404, 486
NlaIII CATG 3 cut(s) 16, 52, 489
NlaIV GGNNCC 2 cut(s) 264, 520
NmeAIII GCCGAG 1 cut(s) 60
NspV TTCGAA 2 cut(s) 88, 217
PaeR7I CTCGAG 1 cut(s) 292
PfeI GAWTC 2 cut(s) 158, 730
PfoI TCCNGGA 1 cut(s) 703
PleI GAGTC 2 cut(s) 146, 503
PpsI GAGTC 2 cut(s) 146, 503
PpuMI RGGWCCY 1 cut(s) 263
Psp5II RGGWCCY 1 cut(s) 263
Psp6I CCWGG 3 cut(s) 523, 536, 703
PspFI CCCAGC 1 cut(s) 339
PspGI CCWGG 3 cut(s) 523, 536, 703
PspN4I GGNNCC 2 cut(s) 264, 520
PspPI GGNCC 2 cut(s) 263, 297
PspPPI RGGWCCY 1 cut(s) 263
PspXI VCTCGAGB 1 cut(s) 292
RsaI GTAC 2 cut(s) 173, 671
RsaNI GTAC 2 cut(s) 172, 670
Sau96I GGNCC 2 cut(s) 263, 297
SchI GAGTC 2 cut(s) 146, 503
ScrFI CCNGG 3 cut(s) 525, 538, 705
SduI GDGCHC 2 cut(s) 323, 470
SetI ASST 8 cut(s) 57, 70, 123, 186, 311, 494, 637, 713
Sfr274I CTCGAG 1 cut(s) 292
SfuI TTCGAA 2 cut(s) 88, 217
SinI GGWCC 1 cut(s) 263
SlaI CTCGAG 1 cut(s) 292
SmlI CTYRAG 2 cut(s) 200, 292
SmoI CTYRAG 2 cut(s) 200, 292
Sse9I AATT 2 cut(s) 60, 301
SsiI CCGC 8 cut(s) 134, 193, 196, 209, 212, 281, 329, 541
SspMI CTAG 1 cut(s) 107
StyD4I CCNGG 3 cut(s) 523, 536, 703
TaqI TCGA 4 cut(s) 88, 217, 293, 305
TasI AATT 2 cut(s) 60, 301
TatI WGTACW 2 cut(s) 171, 669
TauI GCSGC 5 cut(s) 136, 195, 198, 211, 214
TfiI GAWTC 2 cut(s) 158, 730
TscAI CASTG 1 cut(s) 694
TseI GCWGC 5 cut(s) 130, 136, 139, 276, 489
TspDTI ATGAA 3 cut(s) 29, 414, 729
TspRI CASTG 1 cut(s) 694
VpaK11BI GGWCC 1 cut(s) 263
XhoI CTCGAG 1 cut(s) 292
XspI CTAG 1 cut(s) 107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.