RchiOBHm_Chr4g0386031

Cyclin-dependent kinase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0002441
Physical Location & Seq
Forward (+)
1650331 .. 1653682
3352 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35943

Sequence Viewer

Length: 675 bp
ATGGGCAAGTACATGAGGAAGTCCAAGGCCACCGGTGACGTGGCAGTGATGGAAGTCTCGCAGGCCGCCTCTCTCGGGGTTCGCACCCGAGCCAAAACCCTAGCTCTCCAGCGCTCCACCACCTCCTCCGCCTCCTATCTCCAGCTCCGCAGCCGCCGCCTCCAGAAGCACCCGCCGATTCTCCCGCCTCGAGAGCCCCGCAGGCAGAAGCACCACCACCACCACCACACTCCCAAACAGCCAACGCCGGAAAACCCTAACCCGCCCGCCGACGAGGGCTCGCCGGAAAAGGAAGAGGCGCCCGAAGAGGGGCAAAATAATAATGGCGAAAATGGTGATTTCGGTGATGCAGAAGAAGCTTCGTTCGGAGAGAATGTGCCCGAGTTTGAAGGTAGAGAGAGAACCACAAGGGAATCTACACCTTGCAGCTTGATAAGGGATCCAGACACCATCAGAACCCCTGGTTCTACTACTAGGCCTACTAATTCAGCAGATGCTAACCGGAGAGTACAGAACTCATCACAGAGACACATACCAACAGCACATGACATGGATGAGTTCTTTGCTGGTGCTGAAGAAGAGCAGCAGAAGCAATTCATTGACAAATACAACTATGACCCTGTGAATGATAAGCCGCTACCAGGACGTTATGAATGGGAGAAATTGGATCCTTAG

Protein Analysis

224

Amino Acids

25.31

Weight (kDa)

6.16

Isoelectric Point (pI)

54.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDI PF02234 176 - 222 2.3e-19 Cyclin-dependent kinase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 298
AclWI GGATC 3 cut(s) 434, 447, 662
AcuI CTGAAG 1 cut(s) 594
AcyI GRCGYC 1 cut(s) 299
AfaI GTAC 2 cut(s) 11, 510
AfeI AGCGCT 1 cut(s) 113
AfiI CCNNNNNNNGG 4 cut(s) 75, 308, 309, 641
AgeI ACCGGT 1 cut(s) 32
AgsI TTSAA 1 cut(s) 389
AjiI CACGTC 1 cut(s) 40
AjnI CCWGG 2 cut(s) 460, 640
AluBI AGCT 4 cut(s) 104, 145, 359, 429
AluI AGCT 4 cut(s) 104, 145, 359, 429
Alw26I GTCTC 2 cut(s) 61, 520
AlwI GGATC 3 cut(s) 434, 447, 662
Ama87I CYCGRG 4 cut(s) 74, 87, 189, 380
Aor51HI AGCGCT 1 cut(s) 113
AoxI GGCC 3 cut(s) 27, 63, 476
ApeKI GCWGC 3 cut(s) 150, 426, 583
AsiGI ACCGGT 1 cut(s) 32
Asp700I GAANNNNTTC 1 cut(s) 593
AspLEI GCGC 2 cut(s) 114, 301
AsuHPI GGTGA 3 cut(s) 47, 347, 356
AvaI CYCGRG 4 cut(s) 74, 87, 189, 380
BaeGI GKGCMC 1 cut(s) 381
BamHI GGATCC 2 cut(s) 439, 667
BanI GGYRCC 1 cut(s) 298
BanII GRGCYC 2 cut(s) 198, 281
BbvI GCAGC 3 cut(s) 162, 438, 595
BccI CCATC 2 cut(s) 43, 458
BciT130I CCWGG 2 cut(s) 462, 642
BcoDI GTCTC 2 cut(s) 61, 520
BfaI CTAG 2 cut(s) 101, 474
BfoI RGCGCY 2 cut(s) 115, 302
BglI GCCNNNNNGGC 1 cut(s) 202
BisI GCNGC 7 cut(s) 66, 151, 154, 157, 427, 584, 635
BlsI GCNGC 7 cut(s) 67, 152, 155, 158, 428, 585, 636
Bme1390I CCNGG 2 cut(s) 462, 642
BmeT110I CYCGRG 4 cut(s) 74, 87, 189, 380
BmgBI CACGTC 1 cut(s) 40
BmiI GGNNCC 3 cut(s) 300, 441, 669
BmrFI CCNGG 2 cut(s) 462, 642
BmsI GCATC 2 cut(s) 337, 484
BpmI CTGGAG 3 cut(s) 92, 125, 146
BsaHI GRCGYC 1 cut(s) 299
BsaJI CCNNGG 2 cut(s) 24, 460
BsaWI WCCGGW 2 cut(s) 32, 501
BsaXI ACNNNNNCTCC 2 cut(s) 360, 390
Bsc4I CCNNNNNNNGG 4 cut(s) 75, 308, 309, 641
Bse118I RCCGGY 1 cut(s) 32
BseBI CCWGG 2 cut(s) 462, 642
BseDI CCNNGG 2 cut(s) 24, 460
BseGI GGATG 1 cut(s) 559
BseLI CCNNNNNNNGG 4 cut(s) 75, 308, 309, 641
BseRI GAGGAG 1 cut(s) 115
BseSI GKGCMC 1 cut(s) 381
BseXI GCAGC 3 cut(s) 162, 438, 595
BshFI GGCC 3 cut(s) 29, 65, 478
BshNI GGYRCC 1 cut(s) 298
BshTI ACCGGT 1 cut(s) 32
BsiHKCI CYCGRG 4 cut(s) 74, 87, 189, 380
BsiSI CCGG 4 cut(s) 33, 248, 284, 502
BslI CCNNNNNNNGG 4 cut(s) 75, 308, 309, 641
BsmAI GTCTC 2 cut(s) 61, 520
BsnI GGCC 3 cut(s) 29, 65, 478
BsoBI CYCGRG 4 cut(s) 74, 87, 189, 380
Bsp1286I GDGCHC 3 cut(s) 198, 281, 381
Bsp143I GATC 2 cut(s) 439, 667
BspANI GGCC 3 cut(s) 29, 65, 478
BspLI GGNNCC 3 cut(s) 300, 441, 669
BspPI GGATC 3 cut(s) 434, 447, 662
BspQI GCTCTTC 1 cut(s) 573
BspT107I GGYRCC 1 cut(s) 298
BsrFI RCCGGY 1 cut(s) 32
BssAI RCCGGY 1 cut(s) 32
BssECI CCNNGG 2 cut(s) 24, 460
BssMI GATC 2 cut(s) 439, 667
BssNI GRCGYC 1 cut(s) 299
BssT1I CCWWGG 1 cut(s) 24
Bst2UI CCWGG 2 cut(s) 462, 642
Bst6I CTCTTC 3 cut(s) 288, 300, 573
BstACI GRCGYC 1 cut(s) 299
BstC8I GCNNGC 4 cut(s) 63, 203, 267, 281
BstDEI CTNAG 1 cut(s) 672
BstF5I GGATG 1 cut(s) 559
BstH2I RGCGCY 2 cut(s) 115, 302
BstHHI GCGC 2 cut(s) 114, 301
BstKTI GATC 2 cut(s) 442, 670
BstMAI GTCTC 2 cut(s) 61, 520
BstMBI GATC 2 cut(s) 439, 667
BstMWI GCNNNNNNNGC 5 cut(s) 156, 193, 202, 356, 589
BstNI CCWGG 2 cut(s) 462, 642
BstSCI CCNGG 2 cut(s) 460, 640
BstSLI GKGCMC 1 cut(s) 381
BstV1I GCAGC 3 cut(s) 162, 438, 595
BstX2I RGATCY 2 cut(s) 439, 667
BstYI RGATCY 2 cut(s) 439, 667
BsuRI GGCC 3 cut(s) 29, 65, 478
BtrI CACGTC 1 cut(s) 40
BtsCI GGATG 1 cut(s) 559
BtsI GCAGTG 1 cut(s) 51
BtsIMutI CAGTG 1 cut(s) 51
Cac8I GCNNGC 4 cut(s) 63, 203, 267, 281
CfoI GCGC 2 cut(s) 114, 301
Cfr10I RCCGGY 1 cut(s) 32
Csp6I GTAC 2 cut(s) 10, 509
CspAI ACCGGT 1 cut(s) 32
CviAII CATG 3 cut(s) 13, 545, 550
CviQI GTAC 2 cut(s) 10, 509
DdeI CTNAG 1 cut(s) 672
DinI GGCGCC 1 cut(s) 300
DpnI GATC 2 cut(s) 441, 669
DpnII GATC 2 cut(s) 439, 667
Eam1104I CTCTTC 3 cut(s) 288, 300, 573
EarI CTCTTC 3 cut(s) 288, 300, 573
EciI GGCGGA 1 cut(s) 118
Eco130I CCWWGG 1 cut(s) 24
Eco147I AGGCCT 1 cut(s) 478
Eco24I GRGCYC 2 cut(s) 198, 281
Eco47III AGCGCT 1 cut(s) 113
Eco57I CTGAAG 1 cut(s) 594
Eco88I CYCGRG 4 cut(s) 74, 87, 189, 380
EcoRII CCWGG 2 cut(s) 460, 640
EcoT14I CCWWGG 1 cut(s) 24
EcoT38I GRGCYC 2 cut(s) 198, 281
EgeI GGCGCC 1 cut(s) 300
EheI GGCGCC 1 cut(s) 300
ErhI CCWWGG 1 cut(s) 24
FaeI CATG 3 cut(s) 16, 548, 553
FaiI YATR 6 cut(s) 14, 533, 546, 551, 615, 651
FatI CATG 3 cut(s) 12, 544, 549
FauI CCCGC 5 cut(s) 180, 192, 206, 270, 274
Fnu4HI GCNGC 7 cut(s) 66, 151, 154, 157, 427, 584, 635
FokI GGATG 1 cut(s) 566
FriOI GRGCYC 2 cut(s) 198, 281
Fsp4HI GCNGC 7 cut(s) 66, 151, 154, 157, 427, 584, 635
FspBI CTAG 2 cut(s) 101, 474
GlaI GCGC 2 cut(s) 113, 300
GluI GCNGC 7 cut(s) 66, 151, 154, 157, 427, 584, 635
GsuI CTGGAG 3 cut(s) 92, 125, 146
HaeII RGCGCY 2 cut(s) 115, 302
HaeIII GGCC 3 cut(s) 29, 65, 478
HapII CCGG 4 cut(s) 33, 248, 284, 502
HhaI GCGC 2 cut(s) 114, 301
Hin1I GRCGYC 1 cut(s) 299
Hin1II CATG 3 cut(s) 16, 548, 553
Hin6I GCGC 2 cut(s) 112, 299
HinP1I GCGC 2 cut(s) 112, 299
HindIII AAGCTT 1 cut(s) 357
HinfI GANTC 2 cut(s) 178, 413
HpaII CCGG 4 cut(s) 33, 248, 284, 502
HphI GGTGA 3 cut(s) 47, 347, 356
Hpy188I TCNGA 2 cut(s) 368, 455
Hpy188III TCNNGA 3 cut(s) 163, 191, 443
Hpy99I CGWCG 1 cut(s) 275
HpyAV CCTTC 1 cut(s) 383
HpyCH4IV ACGT 2 cut(s) 39, 646
HpyCH4V TGCA 2 cut(s) 350, 426
HpyF10VI GCNNNNNNNGC 5 cut(s) 156, 193, 202, 356, 589
HpyF3I CTNAG 1 cut(s) 672
HpySE526I ACGT 2 cut(s) 39, 646
Hsp92I GRCGYC 1 cut(s) 299
Hsp92II CATG 3 cut(s) 16, 548, 553
HspAI GCGC 2 cut(s) 112, 299
KasI GGCGCC 1 cut(s) 298
Kzo9I GATC 2 cut(s) 439, 667
LguI GCTCTTC 1 cut(s) 573
LmnI GCTCC 2 cut(s) 119, 150
Lsp1109I GCAGC 3 cut(s) 162, 438, 595
LweI GCATC 2 cut(s) 337, 484
MaeI CTAG 2 cut(s) 101, 474
MaeII ACGT 2 cut(s) 39, 646
MaeIII GTNAC 1 cut(s) 35
MalI GATC 2 cut(s) 441, 669
MboI GATC 2 cut(s) 439, 667
MboII GAAGA 5 cut(s) 305, 317, 365, 587, 590
MflI RGATCY 2 cut(s) 439, 667
MhlI GDGCHC 3 cut(s) 198, 281, 381
MluCI AATT 3 cut(s) 484, 593, 662
Mly113I GGCGCC 1 cut(s) 299
MroXI GAANNNNTTC 1 cut(s) 593
MspI CCGG 4 cut(s) 33, 248, 284, 502
MspR9I CCNGG 2 cut(s) 462, 642
MvaI CCWGG 2 cut(s) 462, 642
MwoI GCNNNNNNNGC 5 cut(s) 156, 193, 202, 356, 589
NarI GGCGCC 1 cut(s) 299
NdeII GATC 2 cut(s) 439, 667
NlaIII CATG 3 cut(s) 16, 548, 553
NlaIV GGNNCC 3 cut(s) 300, 441, 669
NmuCI GTSAC 1 cut(s) 35
PaeR7I CTCGAG 1 cut(s) 189
PceI AGGCCT 1 cut(s) 478
PciSI GCTCTTC 1 cut(s) 573
PdmI GAANNNNTTC 1 cut(s) 593
PfeI GAWTC 2 cut(s) 178, 413
PinAI ACCGGT 1 cut(s) 32
PkrI GCNGC 7 cut(s) 67, 152, 155, 158, 428, 585, 636
PluTI GGCGCC 1 cut(s) 302
Psp6I CCWGG 2 cut(s) 460, 640
PspGI CCWGG 2 cut(s) 460, 640
PspN4I GGNNCC 3 cut(s) 300, 441, 669
PsuI RGATCY 2 cut(s) 439, 667
RsaI GTAC 2 cut(s) 11, 510
RsaNI GTAC 2 cut(s) 10, 509
SapI GCTCTTC 1 cut(s) 573
SatI GCNGC 7 cut(s) 66, 151, 154, 157, 427, 584, 635
Sau3AI GATC 2 cut(s) 439, 667
ScrFI CCNGG 2 cut(s) 462, 642
SduI GDGCHC 3 cut(s) 198, 281, 381
SetI ASST 9 cut(s) 42, 106, 125, 147, 361, 394, 424, 431, 649
SfaNI GCATC 2 cut(s) 337, 484
SfoI GGCGCC 1 cut(s) 300
Sfr274I CTCGAG 1 cut(s) 189
SgrAI CRCCGGYG 1 cut(s) 32
SlaI CTCGAG 1 cut(s) 189
SmlI CTYRAG 1 cut(s) 189
SmoI CTYRAG 1 cut(s) 189
Sse9I AATT 3 cut(s) 484, 593, 662
SseBI AGGCCT 1 cut(s) 478
SspDI GGCGCC 1 cut(s) 298
SspMI CTAG 2 cut(s) 101, 474
StuI AGGCCT 1 cut(s) 478
StyD4I CCNGG 2 cut(s) 460, 640
StyI CCWWGG 1 cut(s) 24
TaiI ACGT 2 cut(s) 42, 649
TaqI TCGA 1 cut(s) 190
TasI AATT 3 cut(s) 484, 593, 662
TatI WGTACW 2 cut(s) 9, 508
TauI GCSGC 4 cut(s) 68, 156, 159, 637
TfiI GAWTC 2 cut(s) 178, 413
TscAI CASTG 1 cut(s) 51
TseFI GTSAC 1 cut(s) 35
TseI GCWGC 3 cut(s) 150, 426, 583
Tsp45I GTSAC 1 cut(s) 35
TspDTI ATGAA 2 cut(s) 586, 666
TspRI CASTG 1 cut(s) 51
XcmI CCANNNNNNNNNTGG 1 cut(s) 37
XhoI CTCGAG 1 cut(s) 189
XmnI GAANNNNTTC 1 cut(s) 593
XspI CTAG 2 cut(s) 101, 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.