RLG00000012702

Cyclin-dependent kinase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0002441
Physical Location & Seq
Forward (+)
21472100 .. 21474481
2382 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012702

Sequence Viewer

Length: 729 bp
ATGGGAAAATACATGAAGAAATCCAAAATCACAGGCGACATCGCCGTCATGGAGGTTTCAATTTCACCTCAGTCCTCCCTCGGCGTTCGAACCAGAGCCAAAACCCTAGCCCTGCAGAAGCTCCACCAGCAGCAGCAGCAATCGCCCGACTCCGATTCCTCGTCTCTGTACTATCTCCAGCTCCGAAGCCGCCGCCTTGAGAAGCCGCCGCTGCGAAGCGACGCCAACAAGCAGGCTCAGGCTCAGGCTCAGGCTCCTCGGGATTGCTGCGGCGCGGGGGGCAGGGCCGTGAATTCGAGGTTGAGTGTGGGCTCCTCGGCGGATTTGGGCTGGGATGAGAGGAAGGAGAAGGACGGGGAGGGGTGTTTTGGTAATGTTGAAAGTGAAGCAGAGTATGCCAACGATGTTGAGGCTTCATTTGGGGAAAACAACTTGGATTTTGATGGCAGAGATAGGAGCACCCGAGAAAGCACGCCATGCAGCTTGATCAGGAACTCGGACTCAGTTGGAACCCCTGGTTCTACAACAAGGCGGTCAAGTTCTATTGCAAATAACCAAAGAGTTAGAAATGATACCCAAAGAACCATTCCAACTGCACTTGATATGGACGAGTTCTTTGCCCAAGCTGAGCGGCAGCAACAGAAACTCTTTATGGAGAAGTACAACTTTGATATTACCAGTGATATGCCCCTTCCAGGAAGGTATGAATGGGAGCCAGTGATTCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CDI

Protein Analysis

243

Amino Acids

26.97

Weight (kDa)

6.02

Isoelectric Point (pI)

55.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDI PF02234 195 - 240 4.3e-18 Cyclin-dependent kinase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 44
AccBSI CCGCTC 1 cut(s) 631
AccII CGCG 1 cut(s) 275
AciI CCGC 9 cut(s) 190, 193, 206, 209, 270, 275, 320, 532, 631
AcsI RAATTY 1 cut(s) 292
AcyI GRCGYC 1 cut(s) 222
AfaI GTAC 2 cut(s) 170, 662
AfiI CCNNNNNNNGG 1 cut(s) 695
AgsI TTSAA 2 cut(s) 60, 380
AjnI CCWGG 2 cut(s) 514, 694
AjuI GAANNNNNNNTTGG 2 cut(s) 570, 602
AluBI AGCT 4 cut(s) 121, 181, 483, 626
AluI AGCT 4 cut(s) 121, 181, 483, 626
Alw21I GWGCWC 1 cut(s) 461
Alw26I GTCTC 1 cut(s) 168
Ama87I CYCGRG 2 cut(s) 258, 462
AoxI GGCC 1 cut(s) 285
ApeKI GCWGC 7 cut(s) 130, 133, 136, 211, 267, 480, 634
ApoI RAATTY 1 cut(s) 292
ArsI GACNNNNNNTTYG 2 cut(s) 599, 631
AspLEI GCGC 1 cut(s) 275
AspS9I GGNCC 1 cut(s) 285
AsuHPI GGTGA 1 cut(s) 57
AsuII TTCGAA 1 cut(s) 88
AvaI CYCGRG 2 cut(s) 258, 462
BanII GRGCYC 1 cut(s) 314
Bbv12I GWGCWC 1 cut(s) 461
BbvI GCAGC 7 cut(s) 142, 145, 148, 198, 254, 492, 646
BccI CCATC 1 cut(s) 437
BceAI ACGGC 2 cut(s) 29, 272
BcgI CGANNNNNNTGC 2 cut(s) 599, 633
BciT130I CCWGG 2 cut(s) 516, 696
BclI TGATCA 1 cut(s) 486
BcoDI GTCTC 1 cut(s) 168
BfaI CTAG 1 cut(s) 107
BfmI CTRYAG 1 cut(s) 113
BlpI GCTNAGC 1 cut(s) 627
Bme1390I CCNGG 2 cut(s) 516, 696
BmeT110I CYCGRG 2 cut(s) 258, 462
BmgT120I GGNCC 1 cut(s) 285
BmiI GGNNCC 4 cut(s) 255, 313, 511, 714
BmrFI CCNGG 2 cut(s) 516, 696
BpmI CTGGAG 1 cut(s) 161
Bpu10I CCTNAGC 3 cut(s) 237, 243, 249
Bpu1102I GCTNAGC 1 cut(s) 627
Bpu14I TTCGAA 1 cut(s) 88
BpuEI CTTGAG 1 cut(s) 218
BsaHI GRCGYC 1 cut(s) 222
BsaJI CCNNGG 4 cut(s) 79, 257, 315, 514
Bsc4I CCNNNNNNNGG 1 cut(s) 695
Bse1I ACTGG 2 cut(s) 678, 716
BseBI CCWGG 2 cut(s) 516, 696
BseDI CCNNGG 4 cut(s) 79, 257, 315, 514
BseGI GGATG 1 cut(s) 340
BseLI CCNNNNNNNGG 1 cut(s) 695
BseMII CTCAG 6 cut(s) 83, 251, 257, 263, 516, 618
BseNI ACTGG 2 cut(s) 678, 716
BseRI GAGGAG 2 cut(s) 246, 304
BseXI GCAGC 7 cut(s) 142, 145, 148, 198, 254, 492, 646
BseYI CCCAGC 1 cut(s) 330
BsgI GTGCAG 1 cut(s) 579
Bsh1236I CGCG 1 cut(s) 275
BshFI GGCC 1 cut(s) 287
BsiHKAI GWGCWC 1 cut(s) 461
BsiHKCI CYCGRG 2 cut(s) 258, 462
BslI CCNNNNNNNGG 1 cut(s) 695
BsmAI GTCTC 1 cut(s) 168
BsmBI CGTCTC 1 cut(s) 168
BsnI GGCC 1 cut(s) 287
BsoBI CYCGRG 2 cut(s) 258, 462
Bsp119I TTCGAA 1 cut(s) 88
Bsp1286I GDGCHC 2 cut(s) 314, 461
Bsp143I GATC 1 cut(s) 486
Bsp1720I GCTNAGC 1 cut(s) 627
BspACI CCGC 9 cut(s) 190, 193, 206, 209, 270, 275, 320, 532, 631
BspANI GGCC 1 cut(s) 287
BspCNI CTCAG 6 cut(s) 82, 250, 256, 262, 515, 619
BspFNI CGCG 1 cut(s) 275
BspLI GGNNCC 4 cut(s) 255, 313, 511, 714
BspMAI CTGCAG 1 cut(s) 117
BspT104I TTCGAA 1 cut(s) 88
BsrBI CCGCTC 1 cut(s) 631
BsrI ACTGG 2 cut(s) 678, 716
BssECI CCNNGG 4 cut(s) 79, 257, 315, 514
BssMI GATC 1 cut(s) 486
BssNI GRCGYC 1 cut(s) 222
Bst2UI CCWGG 2 cut(s) 516, 696
BstACI GRCGYC 1 cut(s) 222
BstAPI GCANNNNNTGC 2 cut(s) 395, 477
BstBI TTCGAA 1 cut(s) 88
BstC8I GCNNGC 2 cut(s) 234, 473
BstDEI CTNAG 6 cut(s) 69, 237, 243, 249, 502, 627
BstF5I GGATG 1 cut(s) 340
BstFNI CGCG 1 cut(s) 275
BstHHI GCGC 1 cut(s) 275
BstKTI GATC 1 cut(s) 489
BstMAI GTCTC 1 cut(s) 168
BstMBI GATC 1 cut(s) 486
BstMWI GCNNNNNNNGC 7 cut(s) 127, 136, 142, 211, 279, 395, 477
BstNI CCWGG 2 cut(s) 516, 696
BstSCI CCNGG 2 cut(s) 514, 694
BstSFI CTRYAG 1 cut(s) 113
BstUI CGCG 1 cut(s) 275
BstV1I GCAGC 7 cut(s) 142, 145, 148, 198, 254, 492, 646
BsuRI GGCC 1 cut(s) 287
BtgZI GCGATG 1 cut(s) 25
BtsCI GGATG 1 cut(s) 340
BtsIMutI CAGTG 2 cut(s) 685, 723
Cac8I GCNNGC 2 cut(s) 234, 473
CfoI GCGC 1 cut(s) 275
Cfr13I GGNCC 1 cut(s) 285
CseI GACGC 1 cut(s) 230
Csp6I GTAC 2 cut(s) 169, 661
CviAII CATG 3 cut(s) 13, 49, 477
CviQI GTAC 2 cut(s) 169, 661
DdeI CTNAG 6 cut(s) 69, 237, 243, 249, 502, 627
DpnI GATC 1 cut(s) 488
DpnII GATC 1 cut(s) 486
DrdI GACNNNNNNGTC 1 cut(s) 44
DseDI GACNNNNNNGTC 1 cut(s) 44
EciI GGCGGA 1 cut(s) 335
Eco24I GRGCYC 1 cut(s) 314
Eco88I CYCGRG 2 cut(s) 258, 462
EcoRI GAATTC 1 cut(s) 292
EcoRII CCWGG 2 cut(s) 514, 694
EcoT38I GRGCYC 1 cut(s) 314
Esp3I CGTCTC 1 cut(s) 168
FaeI CATG 3 cut(s) 16, 52, 480
FaiI YATR 8 cut(s) 14, 50, 396, 478, 605, 653, 686, 705
FalI AAGNNNNNCTT 2 cut(s) 650, 682
FatI CATG 3 cut(s) 12, 48, 476
FauI CCCGC 1 cut(s) 268
FbaI TGATCA 1 cut(s) 486
FokI GGATG 1 cut(s) 347
FriOI GRGCYC 1 cut(s) 314
FspBI CTAG 1 cut(s) 107
GlaI GCGC 1 cut(s) 274
GsaI CCCAGC 1 cut(s) 334
GsuI CTGGAG 1 cut(s) 161
HaeIII GGCC 1 cut(s) 287
HgaI GACGC 1 cut(s) 230
HhaI GCGC 1 cut(s) 275
Hin1I GRCGYC 1 cut(s) 222
Hin1II CATG 3 cut(s) 16, 52, 480
Hin6I GCGC 1 cut(s) 273
HinP1I GCGC 1 cut(s) 273
HinfI GANTC 4 cut(s) 149, 155, 500, 721
HphI GGTGA 1 cut(s) 57
Hpy188I TCNGA 3 cut(s) 154, 185, 499
Hpy188III TCNNGA 2 cut(s) 260, 490
Hpy99I CGWCG 1 cut(s) 224
HpyAV CCTTC 4 cut(s) 337, 343, 693, 701
HpyCH4V TGCA 4 cut(s) 115, 480, 548, 596
HpyF10VI GCNNNNNNNGC 7 cut(s) 127, 136, 142, 211, 279, 395, 477
HpyF3I CTNAG 6 cut(s) 69, 237, 243, 249, 502, 627
Hsp92I GRCGYC 1 cut(s) 222
Hsp92II CATG 3 cut(s) 16, 52, 480
HspAI GCGC 1 cut(s) 273
Ksp22I TGATCA 1 cut(s) 486
Kzo9I GATC 1 cut(s) 486
LmnI GCTCC 6 cut(s) 126, 186, 259, 317, 456, 712
Lsp1109I GCAGC 7 cut(s) 142, 145, 148, 198, 254, 492, 646
MaeI CTAG 1 cut(s) 107
MalI GATC 1 cut(s) 488
MbiI CCGCTC 1 cut(s) 631
MboI GATC 1 cut(s) 486
MboII GAAGA 1 cut(s) 28
MhlI GDGCHC 2 cut(s) 314, 461
MluCI AATT 2 cut(s) 60, 292
MlyI GAGTC 2 cut(s) 143, 494
MmeI TCCRAC 2 cut(s) 487, 614
MspA1I CMGCKG 1 cut(s) 211
MspR9I CCNGG 2 cut(s) 516, 696
MvaI CCWGG 2 cut(s) 516, 696
MvnI CGCG 1 cut(s) 275
MwoI GCNNNNNNNGC 7 cut(s) 127, 136, 142, 211, 279, 395, 477
NdeII GATC 1 cut(s) 486
NlaIII CATG 3 cut(s) 16, 52, 480
NlaIV GGNNCC 4 cut(s) 255, 313, 511, 714
NmeAIII GCCGAG 2 cut(s) 60, 296
NspV TTCGAA 1 cut(s) 88
PfeI GAWTC 2 cut(s) 155, 721
PfoI TCCNGGA 1 cut(s) 694
PleI GAGTC 2 cut(s) 143, 494
PpsI GAGTC 2 cut(s) 143, 494
Psp6I CCWGG 2 cut(s) 514, 694
PspFI CCCAGC 1 cut(s) 330
PspGI CCWGG 2 cut(s) 514, 694
PspN4I GGNNCC 4 cut(s) 255, 313, 511, 714
PspPI GGNCC 1 cut(s) 285
PstI CTGCAG 1 cut(s) 117
RsaI GTAC 2 cut(s) 170, 662
RsaNI GTAC 2 cut(s) 169, 661
Sau3AI GATC 1 cut(s) 486
Sau96I GGNCC 1 cut(s) 285
SchI GAGTC 2 cut(s) 143, 494
ScrFI CCNGG 2 cut(s) 516, 696
SduI GDGCHC 2 cut(s) 314, 461
SetI ASST 8 cut(s) 57, 70, 123, 183, 302, 485, 628, 704
SfcI CTRYAG 1 cut(s) 113
SfuI TTCGAA 1 cut(s) 88
SmlI CTYRAG 1 cut(s) 197
SmoI CTYRAG 1 cut(s) 197
Sse9I AATT 2 cut(s) 60, 292
SsiI CCGC 9 cut(s) 190, 193, 206, 209, 270, 275, 320, 532, 631
SspMI CTAG 1 cut(s) 107
StyD4I CCNGG 2 cut(s) 514, 694
TaqI TCGA 2 cut(s) 88, 296
TasI AATT 2 cut(s) 60, 292
TatI WGTACW 2 cut(s) 168, 660
TauI GCSGC 6 cut(s) 192, 195, 208, 211, 273, 634
TfiI GAWTC 2 cut(s) 155, 721
TscAI CASTG 2 cut(s) 685, 723
TseI GCWGC 7 cut(s) 130, 133, 136, 211, 267, 480, 634
TspDTI ATGAA 3 cut(s) 29, 405, 720
TspRI CASTG 2 cut(s) 685, 723
XapI RAATTY 1 cut(s) 292
XspI CTAG 1 cut(s) 107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.