FvH4_2g20210

WD domain, G-beta repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001434
Physical Location & Seq
Forward (+)
16996148 .. 17002840
6693 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g20210.t5

Sequence Viewer

Length: 2262 bp
ATGAAGTCCGATCTTATGATGACCGGAGCTTCACCGCCGTCCGATCAGTCCACGTCGCCGGCCGAAAAGCATCACTGCAGCTTGATAGTAGGGAGAGAAATTTCTCCTCGAGCAAAACGCTTGCCTAGAAGACGGTGGGGAGAAACTCCTAAAGTGAAAGCTGGTTCCTCTTCCACTGGCCCTGAGTGGCAAGCAACAATAGATGCTAAACGTGGCCTTCTTTCATGGGTTGAAGCAGAGTCACTACAACATTTGTCAGCGAGGTACTGTGATTTAGTGCCTCCTCCGAGGTCAACCATTGCTGCAGCATTTAGTCCCGATGGAAGAACGGTTGCTTCTACACATGGTGATCACACTGTAAAGATAATTGATTGCCAAACTGGAAGGTGCTTGAAAGTTCTGAGTGGCCATAGGAGGACACCGTGGGTGGTGAGGTTCCACCCTTCACGACCAGAAATAATTGCTAGTGGGGGTTTGGATCACGAAGTTCGCCTATGGGATCTAAACACATCTGAGTGTATAGGATCACGTGATTTCTATCGACCTATTGCATCCATAGCCTTTCATGCGACAGGGGAACTCCTAGTTGTTGCATCAGGACACAAGGTGTATATATGGAACTACAAAAAGCAGCAGTCCTCTCCAGACATTGTATTGAAAACATGGCGTTCACTTAGAGCAGTGCATTTTCACCCCCAAGCTGCTCCGTTTCTTCTAACTGCTGAGGTCAATGATCATGACTCTTCAGATTCCTTGATGACACCAGCAACATCTTCGGGATACTTGCAATATCCTTCTCCTGCTGTTTTCTTGGCAAACGTCCATTCTAGTGAACGCCTTAGTTTGGCTGCTGAACTGCCACAAATGTCTTTGCCTTTCTTCTTTGTGTCACCGTCTGTAGATGGTCCAAGAACAGAAATGCAGGGTGATGGGCATGTTGGTTCAAGTAACATGCAAGTCGCATCTGCCTCACGCCAGTTTGAATCAGATGCAAATGCAACAGAGCAGAGGGATAGCACAGTTTCTCCCATGGAGGCATTTCCATCAGTTCCATCTTTTTCTCATCTCAACGATGCAATGGAGACTGATGAAATGCAGTCTGTAGGGAGAAGCCAGCATGAAAACTCTACCAATGTAGATAATGCTATTGATAGACTACCTGAAAATGTATCAAATCCTCTTCAATTTGCTGAACCTCGGCAACTTCGTCATATGTTTCCTTATAGAGATCCAGCATTTTGGGAGCTTCCTTTTCTCCAGGGATGGTTAACGGGTCAGAGCCAAGCCAGTTTTCCCGCAATGGTTCCCCTTAATGGTGCTGGGCTTGACACTTTAGCCCAACATAATGTTCCTTCTAATTTAGCACCACTTGTGGCACCTTCTTCAGCAATGCGAGGAAGCACCAGCTTATCTGGGGTTTCTGGAAGAGCTGGTTTGCAATTTCGCTTCTCAGTACCGGAATCTGGGGATAGTGCCGCTATCTTAAACGCCCTGCATGATGGGAATGAAGCTCAGCCCATTGTTAGCAGAATCCATTCTGAAATTACTACATCACTGGCTGCTACAGCAGCTGCAGAGTTACCATGCACTGTGAAACTAAGAATATGGTCGCATGACATAACAAATCCTTCTGCTCCACTCAATGCTGAACGATGCCGCTTAACCATTCCCCATGCTGTTCTTTGCAGCGAAATGGGCACTCATTTCTCTCCTTGTGGGAGATACTTAGCTGCCTGTGTTGCATGCATGCTTCCTGATATGGAAGCTGAACCTGGACTGCAAAGTGTAGTCCATCAAGATTCTAGAATTGCAACATCCCCAACCCGGCATCCAATCTCAGCACATCAAGTCATCTATGAGCTTCGCATATATTCACTTGAGAAGGCAACATTTGGTTCACCACTTGCTTCCCGAGCTATCAGAGCTGCACATTGTTTGACTTCGATTCAGTTCTCACCCACGTCTGAGCATATATTAATTGCTTATGGTCGACGCCATGGTTCTCTTCTTAAAAGTATTGTCATTGATGGTGAATCAACAGTACCTATTTACACTGTTCTTGAGATCTACAGAGTTTCAGATATGGAACTTGTGAGAGTACTTCCTAGTGCAGATGATGAGGTTAATGTTGCTTGCTTTCATCCGTTTGCTGGTGGTGGTCTGGCTTATGGAACCAAGGAAGGGAAGCTTAGAGTCCTCCACTTTGATAGTGCTCACGGTGGAAACTGCTTTACAGAGGATAATATGGCTGTCCTGGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000045 GO:0000422 GO:0000423 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0005856 GO:0005929 GO:0005930 GO:0006914 GO:0006950 GO:0006996 GO:0007005 GO:0007033 GO:0007275 GO:0007399 GO:0008150 GO:0008152 GO:0008285 GO:0009056 GO:0009790 GO:0009792 GO:0009888 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010506 GO:0010508 GO:0010562 GO:0010656 GO:0010660 GO:0010662 GO:0010664 GO:0010665 GO:0010667 GO:0010941 GO:0016043 GO:0016236 GO:0019216 GO:0019220 GO:0019222 GO:0019899 GO:0021915 GO:0022411 GO:0022607 GO:0030139 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0031410 GO:0031625 GO:0031982 GO:0032501 GO:0032502 GO:0032838 GO:0033554 GO:0033674 GO:0035295 GO:0042127 GO:0042325 GO:0042327 GO:0042981 GO:0042995 GO:0043009 GO:0043066 GO:0043067 GO:0043069 GO:0043085 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043523 GO:0043524 GO:0043549 GO:0043550 GO:0043551 GO:0043552 GO:0044085 GO:0044093 GO:0044237 GO:0044248 GO:0044389 GO:0044422 GO:0044424 GO:0044430 GO:0044441 GO:0044444 GO:0044446 GO:0044463 GO:0044464 GO:0045335 GO:0045834 GO:0045937 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051020 GO:0051174 GO:0051338 GO:0051347 GO:0051716 GO:0060429 GO:0060548 GO:0061726 GO:0061912 GO:0061919 GO:0065007 GO:0065009 GO:0070925 GO:0071840 GO:0080090 GO:0090218 GO:0097014 GO:0097708 GO:0098780 GO:0099568 GO:0120025 GO:0120038 GO:1901214 GO:1901215 GO:1903008 GO:1903725 GO:1903727 GO:1905037
KEGG Pathways
Metabolic & Signaling
ko04137 ko04140 map04137 map04140
Pfam Domains
Protein Families
WD40

Protein Analysis

754

Amino Acids

82.02

Weight (kDa)

6.31

Isoelectric Point (pI)

53.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Gbeta PF25391 104 - 213 5.2e-06 G protein beta WD-40 repeat protein
Beta-prop_THOC3 PF25174 105 - 238 2.8e-20 THOC3 beta-propeller domain
WDR55 PF24796 105 - 216 1.6e-08 WDR55
Beta-prop_WDR3_1st PF25173 106 - 213 9.4e-13 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 106 - 238 2.2e-12 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 107 - 249 1.7e-21 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 107 - 178 2.2e-09 WDHD1 first WD40 domain
WD40_CDC20-Fz PF24807 107 - 221 3.6e-13 CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 109 - 217 5.2e-09 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR90_POC16_2nd PF23393 112 - 216 1e-05 WDR90/POC16, second beta-propeller
Beta-prop_EML_2 PF23414 146 - 237 1.9e-07 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1375
AccI GTMKAC 1 cut(s) 1990
AciI CCGC 4 cut(s) 35, 1296, 1476, 1657
AclWI GGATC 4 cut(s) 486, 507, 532, 1223
AcoI YGGCCR 2 cut(s) 60, 406
AcsI RAATTY 1 cut(s) 99
AcuI CTGAAG 2 cut(s) 729, 1368
AcvI CACGTG 1 cut(s) 530
AcyI GRCGYC 1 cut(s) 1993
AdeI CACNNNGTG 2 cut(s) 347, 607
AfaI GTAC 4 cut(s) 266, 1455, 2043, 2100
AfiI CCNNNNNNNGG 6 cut(s) 844, 1313, 1463, 1824, 2150, 2181
AgsI TTSAA 6 cut(s) 233, 394, 658, 945, 983, 1184
AjiI CACGTC 2 cut(s) 54, 1962
AjnI CCWGG 3 cut(s) 1257, 1771, 2253
AjuI GAANNNNNNNTTGG 4 cut(s) 1275, 1307, 1332, 1364
Alw21I GWGCWC 1 cut(s) 2215
Alw26I GTCTC 1 cut(s) 1076
AlwI GGATC 4 cut(s) 486, 507, 532, 1223
AlwNI CAGNNNCTG 1 cut(s) 1571
Ama87I CYCGRG 2 cut(s) 108, 1911
AoxI GGCC 4 cut(s) 60, 178, 214, 406
ApoI RAATTY 1 cut(s) 99
AseI ATTAAT 1 cut(s) 1976
Asp700I GAANNNNTTC 1 cut(s) 1534
AspS9I GGNCC 2 cut(s) 179, 905
AsuC2I CCSGG 1 cut(s) 1825
AsuHPI GGTGA 9 cut(s) 24, 359, 442, 683, 882, 938, 1890, 1947, 2042
AvaI CYCGRG 2 cut(s) 108, 1911
AvaII GGWCC 1 cut(s) 905
BaeGI GKGCMC 1 cut(s) 1700
BalI TGGCCA 1 cut(s) 408
BanI GGYRCC 1 cut(s) 1375
BbrPI CACGTG 1 cut(s) 530
BbsI GAAGAC 1 cut(s) 136
Bbv12I GWGCWC 1 cut(s) 2215
BbvCI CCTCAGC 1 cut(s) 723
BccI CCATC 9 cut(s) 314, 896, 923, 1051, 1060, 1257, 1493, 1800, 2021
BceAI ACGGC 1 cut(s) 22
BcgI CGANNNNNNTGC 2 cut(s) 756, 790
BciT130I CCWGG 3 cut(s) 1259, 1773, 2255
BciVI GTATCC 1 cut(s) 773
BclI TGATCA 2 cut(s) 349, 733
BcnI CCSGG 1 cut(s) 1825
BcoDI GTCTC 1 cut(s) 1076
BfaI CTAG 6 cut(s) 126, 465, 584, 828, 1803, 2106
BfmI CTRYAG 7 cut(s) 76, 303, 897, 1101, 1563, 1572, 2068
BfuI GTATCC 1 cut(s) 773
BglII AGATCT 1 cut(s) 2064
BlpI GCTNAGC 1 cut(s) 1512
BmcAI AGTACT 1 cut(s) 2100
Bme1390I CCNGG 4 cut(s) 1259, 1773, 1825, 2255
Bme18I GGWCC 1 cut(s) 905
BmeT110I CYCGRG 2 cut(s) 108, 1911
BmgBI CACGTC 2 cut(s) 54, 1962
BmgT120I GGNCC 2 cut(s) 179, 905
BmiI GGNNCC 5 cut(s) 166, 437, 1305, 1377, 2173
BmrFI CCNGG 4 cut(s) 1259, 1773, 1825, 2255
BmsI GCATC 9 cut(s) 79, 193, 560, 602, 971, 979, 1063, 1643, 1837
BpiI GAAGAC 1 cut(s) 136
BpmI CTGGAG 2 cut(s) 627, 1241
Bpu10I CCTNAGC 1 cut(s) 723
Bpu1102I GCTNAGC 1 cut(s) 1512
BpuEI CTTGAG 2 cut(s) 1898, 2081
BpuMI CCSGG 1 cut(s) 1825
BsaAI YACGTR 1 cut(s) 530
BsaBI GATNNNNATC 1 cut(s) 537
BsaHI GRCGYC 1 cut(s) 1993
BsaJI CCNNGG 7 cut(s) 287, 422, 1029, 1196, 1258, 1996, 2175
BsaWI WCCGGW 2 cut(s) 23, 1456
BsaXI ACNNNNNCTCC 2 cut(s) 1009, 1039
Bsc4I CCNNNNNNNGG 6 cut(s) 844, 1313, 1463, 1824, 2150, 2181
Bse118I RCCGGY 1 cut(s) 58
Bse1I ACTGG 5 cut(s) 181, 385, 976, 1287, 1560
Bse3DI GCAATG 4 cut(s) 297, 1083, 1305, 1395
Bse8I GATNNNNATC 1 cut(s) 537
BseBI CCWGG 3 cut(s) 1259, 1773, 2255
BseDI CCNNGG 7 cut(s) 287, 422, 1029, 1196, 1258, 1996, 2175
BseGI GGATG 5 cut(s) 551, 1268, 1814, 1828, 2140
BseJI GATNNNNATC 1 cut(s) 537
BseLI CCNNNNNNNGG 6 cut(s) 844, 1313, 1463, 1824, 2150, 2181
BseMI GCAATG 4 cut(s) 297, 1083, 1305, 1395
BseMII CTCAG 8 cut(s) 174, 392, 504, 714, 1464, 1526, 1851, 1956
BseNI ACTGG 5 cut(s) 181, 385, 976, 1287, 1560
BseRI GAGGAG 2 cut(s) 96, 273
BseSI GKGCMC 1 cut(s) 1700
BseX3I CGGCCG 1 cut(s) 60
BseYI CCCAGC 1 cut(s) 1319
BsgI GTGCAG 2 cut(s) 1911, 2130
Bsh1285I CGRYCG 1 cut(s) 63
BshFI GGCC 4 cut(s) 62, 180, 216, 408
BshNI GGYRCC 1 cut(s) 1375
BsiEI CGRYCG 1 cut(s) 63
BsiHKAI GWGCWC 1 cut(s) 2215
BsiHKCI CYCGRG 2 cut(s) 108, 1911
BsiSI CCGG 4 cut(s) 24, 59, 1457, 1825
BslFI GGGAC 1 cut(s) 300
BslI CCNNNNNNNGG 6 cut(s) 844, 1313, 1463, 1824, 2150, 2181
BsmAI GTCTC 1 cut(s) 1076
BsmFI GGGAC 1 cut(s) 300
BsnI GGCC 4 cut(s) 62, 180, 216, 408
BsoBI CYCGRG 2 cut(s) 108, 1911
Bsp1286I GDGCHC 2 cut(s) 1700, 2215
Bsp143I GATC 9 cut(s) 10, 43, 349, 478, 499, 524, 733, 1228, 2064
Bsp1720I GCTNAGC 1 cut(s) 1512
Bsp19I CCATGG 2 cut(s) 1029, 1996
BspACI CCGC 4 cut(s) 35, 1296, 1476, 1657
BspANI GGCC 4 cut(s) 62, 180, 216, 408
BspCNI CTCAG 8 cut(s) 175, 393, 505, 715, 1463, 1525, 1850, 1957
BspHI TCATGA 1 cut(s) 736
BspLI GGNNCC 5 cut(s) 166, 437, 1305, 1377, 2173
BspMAI CTGCAG 3 cut(s) 80, 307, 1576
BspPI GGATC 4 cut(s) 486, 507, 532, 1223
BspQI GCTCTTC 1 cut(s) 1420
BspT107I GGYRCC 1 cut(s) 1375
BsrDI GCAATG 4 cut(s) 297, 1083, 1305, 1395
BsrFI RCCGGY 1 cut(s) 58
BsrI ACTGG 5 cut(s) 181, 385, 976, 1287, 1560
BssAI RCCGGY 1 cut(s) 58
BssECI CCNNGG 7 cut(s) 287, 422, 1029, 1196, 1258, 1996, 2175
BssMI GATC 9 cut(s) 10, 43, 349, 478, 499, 524, 733, 1228, 2064
BssNI GRCGYC 1 cut(s) 1993
BssT1I CCWWGG 3 cut(s) 1029, 1996, 2175
Bst2UI CCWGG 3 cut(s) 1259, 1773, 2255
Bst6I CTCTTC 5 cut(s) 175, 748, 1185, 1420, 2010
BstACI GRCGYC 1 cut(s) 1993
BstBAI YACGTR 1 cut(s) 530
BstC8I GCNNGC 7 cut(s) 60, 122, 192, 1115, 1744, 1748, 2134
BstDSI CCRYGG 3 cut(s) 422, 1029, 1996
BstF5I GGATG 5 cut(s) 551, 1268, 1814, 1828, 2140
BstKTI GATC 9 cut(s) 13, 46, 352, 481, 502, 527, 736, 1231, 2067
BstMAI GTCTC 1 cut(s) 1076
BstMBI GATC 9 cut(s) 10, 43, 349, 478, 499, 524, 733, 1228, 2064
BstMCI CGRYCG 1 cut(s) 63
BstMWI GCNNNNNNNGC 8 cut(s) 557, 566, 1565, 1568, 1695, 1739, 1913, 1922
BstNI CCWGG 3 cut(s) 1259, 1773, 2255
BstNSI RCATGY 4 cut(s) 938, 955, 1746, 1750
BstSCI CCNGG 4 cut(s) 1257, 1771, 1823, 2253
BstSFI CTRYAG 7 cut(s) 76, 303, 897, 1101, 1563, 1572, 2068
BstSLI GKGCMC 1 cut(s) 1700
BstV2I GAAGAC 1 cut(s) 136
BstX2I RGATCY 3 cut(s) 499, 1228, 2064
BstXI CCANNNNNNTGG 1 cut(s) 1239
BstYI RGATCY 3 cut(s) 499, 1228, 2064
BstZI CGGCCG 1 cut(s) 60
BsuI GTATCC 1 cut(s) 773
BsuRI GGCC 4 cut(s) 62, 180, 216, 408
BtgI CCRYGG 3 cut(s) 422, 1029, 1996
BtrI CACGTC 2 cut(s) 54, 1962
BtsCI GGATG 5 cut(s) 551, 1268, 1814, 1828, 2140
BtsI GCAGTG 2 cut(s) 73, 687
BtsIMutI CAGTG 7 cut(s) 73, 174, 354, 687, 1553, 1587, 2052
Cac8I GCNNGC 7 cut(s) 60, 122, 192, 1115, 1744, 1748, 2134
CaiI CAGNNNCTG 1 cut(s) 1571
CciI TCATGA 1 cut(s) 736
Cfr10I RCCGGY 1 cut(s) 58
Cfr13I GGNCC 2 cut(s) 179, 905
CseI GACGC 1 cut(s) 2001
Csp6I GTAC 4 cut(s) 265, 1454, 2042, 2099
CviQI GTAC 4 cut(s) 265, 1454, 2042, 2099
DpnI GATC 9 cut(s) 12, 45, 351, 480, 501, 526, 735, 1230, 2066
DpnII GATC 9 cut(s) 10, 43, 349, 478, 499, 524, 733, 1228, 2064
DraIII CACNNNGTG 2 cut(s) 347, 607
EaeI YGGCCR 2 cut(s) 60, 406
EagI CGGCCG 1 cut(s) 60
Eam1104I CTCTTC 5 cut(s) 175, 748, 1185, 1420, 2010
EarI CTCTTC 5 cut(s) 175, 748, 1185, 1420, 2010
EclXI CGGCCG 1 cut(s) 60
Eco130I CCWWGG 3 cut(s) 1029, 1996, 2175
Eco47I GGWCC 1 cut(s) 905
Eco52I CGGCCG 1 cut(s) 60
Eco57I CTGAAG 2 cut(s) 729, 1368
Eco72I CACGTG 1 cut(s) 530
Eco88I CYCGRG 2 cut(s) 108, 1911
EcoRII CCWGG 3 cut(s) 1257, 1771, 2253
EcoT14I CCWWGG 3 cut(s) 1029, 1996, 2175
EcoT22I ATGCAT 1 cut(s) 1748
ErhI CCWWGG 3 cut(s) 1029, 1996, 2175
FalI AAGNNNNNCTT 2 cut(s) 2172, 2204
FaqI GGGAC 1 cut(s) 300
FauI CCCGC 1 cut(s) 1303
FauNDI CATATG 1 cut(s) 1212
FbaI TGATCA 2 cut(s) 349, 733
FblI GTMKAC 1 cut(s) 1990
FokI GGATG 5 cut(s) 538, 1275, 1801, 1815, 2127
FspBI CTAG 6 cut(s) 126, 465, 584, 828, 1803, 2106
GsaI CCCAGC 1 cut(s) 1323
GsuI CTGGAG 2 cut(s) 627, 1241
HaeIII GGCC 4 cut(s) 62, 180, 216, 408
HapII CCGG 4 cut(s) 24, 59, 1457, 1825
HgaI GACGC 1 cut(s) 2001
Hin1I GRCGYC 1 cut(s) 1993
HincII GTYRAC 3 cut(s) 294, 1269, 1991
HindII GTYRAC 3 cut(s) 294, 1269, 1991
HindIII AAGCTT 1 cut(s) 2186
HpaI GTTAAC 1 cut(s) 1269
HpaII CCGG 4 cut(s) 24, 59, 1457, 1825
HphI GGTGA 9 cut(s) 24, 359, 442, 683, 882, 938, 1890, 1947, 2042
Hpy166II GTNNAC 7 cut(s) 51, 294, 671, 833, 1269, 1898, 1991
Hpy8I GTNNAC 7 cut(s) 51, 294, 671, 833, 1269, 1898, 1991
Hpy99I CGWCG 2 cut(s) 58, 1995
HpyAV CCTTC 9 cut(s) 227, 378, 453, 804, 1362, 1389, 1638, 1876, 2174
HpyCH4IV ACGT 5 cut(s) 53, 211, 529, 819, 1961
HpyF10VI GCNNNNNNNGC 8 cut(s) 557, 566, 1565, 1568, 1695, 1739, 1913, 1922
HpySE526I ACGT 5 cut(s) 53, 211, 529, 819, 1961
Hsp92I GRCGYC 1 cut(s) 1993
KroI GCCGGC 1 cut(s) 58
KroNI GCCGGC 1 cut(s) 60
Ksp22I TGATCA 2 cut(s) 349, 733
KspAI GTTAAC 1 cut(s) 1269
Kzo9I GATC 9 cut(s) 10, 43, 349, 478, 499, 524, 733, 1228, 2064
LguI GCTCTTC 1 cut(s) 1420
LmnI GCTCC 4 cut(s) 26, 709, 1243, 1639
LweI GCATC 9 cut(s) 79, 193, 560, 602, 971, 979, 1063, 1643, 1837
MaeI CTAG 6 cut(s) 126, 465, 584, 828, 1803, 2106
MaeII ACGT 5 cut(s) 53, 211, 529, 819, 1961
MaeIII GTNAC 4 cut(s) 240, 888, 947, 1578
MalI GATC 9 cut(s) 12, 45, 351, 480, 501, 526, 735, 1230, 2066
MboI GATC 9 cut(s) 10, 43, 349, 478, 499, 524, 733, 1228, 2064
MflI RGATCY 3 cut(s) 499, 1228, 2064
MhlI GDGCHC 2 cut(s) 1700, 2215
MlsI TGGCCA 1 cut(s) 408
MluCI AATT 9 cut(s) 99, 366, 459, 1184, 1357, 1439, 1542, 1806, 1977
MluNI TGGCCA 1 cut(s) 408
MlyI GAGTC 3 cut(s) 248, 734, 2202
Mox20I TGGCCA 1 cut(s) 408
Mph1103I ATGCAT 1 cut(s) 1748
MroNI GCCGGC 1 cut(s) 58
MroXI GAANNNNTTC 1 cut(s) 1534
MscI TGGCCA 1 cut(s) 408
MseI TTAA 7 cut(s) 1268, 1311, 1484, 1661, 1976, 2010, 2124
MslI CAYNNNNRTG 2 cut(s) 514, 828
Msp20I TGGCCA 1 cut(s) 408
MspA1I CMGCKG 1 cut(s) 1571
MspI CCGG 4 cut(s) 24, 59, 1457, 1825
MspR9I CCNGG 4 cut(s) 1259, 1773, 1825, 2255
MvaI CCWGG 3 cut(s) 1259, 1773, 2255
MwoI GCNNNNNNNGC 8 cut(s) 557, 566, 1565, 1568, 1695, 1739, 1913, 1922
NaeI GCCGGC 1 cut(s) 60
NciI CCSGG 1 cut(s) 1825
NcoI CCATGG 2 cut(s) 1029, 1996
NdeI CATATG 1 cut(s) 1212
NdeII GATC 9 cut(s) 10, 43, 349, 478, 499, 524, 733, 1228, 2064
NgoMIV GCCGGC 1 cut(s) 58
NlaIV GGNNCC 5 cut(s) 166, 437, 1305, 1377, 2173
NmeAIII GCCGAG 1 cut(s) 1177
NmuCI GTSAC 2 cut(s) 240, 888
NsiI ATGCAT 1 cut(s) 1748
NspI RCATGY 4 cut(s) 938, 955, 1746, 1750
PaeI GCATGC 2 cut(s) 1746, 1750
PaeR7I CTCGAG 1 cut(s) 108
PagI TCATGA 1 cut(s) 736
PciSI GCTCTTC 1 cut(s) 1420
PcsI WCGNNNNNNNCGW 1 cut(s) 1204
PdiI GCCGGC 1 cut(s) 60
PdmI GAANNNNTTC 1 cut(s) 1534
PfeI GAWTC 7 cut(s) 749, 983, 1460, 1530, 1799, 1945, 2033
PleI GAGTC 3 cut(s) 247, 734, 2201
PmaCI CACGTG 1 cut(s) 530
PmlI CACGTG 1 cut(s) 530
PpsI GAGTC 3 cut(s) 247, 734, 2201
Ppu21I YACGTR 1 cut(s) 530
PshBI ATTAAT 1 cut(s) 1976
Psp6I CCWGG 3 cut(s) 1257, 1771, 2253
PspCI CACGTG 1 cut(s) 530
PspFI CCCAGC 1 cut(s) 1319
PspGI CCWGG 3 cut(s) 1257, 1771, 2253
PspN4I GGNNCC 5 cut(s) 166, 437, 1305, 1377, 2173
PspPI GGNCC 2 cut(s) 179, 905
PspXI VCTCGAGB 1 cut(s) 108
PstI CTGCAG 3 cut(s) 80, 307, 1576
PstNI CAGNNNCTG 1 cut(s) 1571
PsuI RGATCY 3 cut(s) 499, 1228, 2064
PvuII CAGCTG 1 cut(s) 1571
RsaI GTAC 4 cut(s) 266, 1455, 2043, 2100
RsaNI GTAC 4 cut(s) 265, 1454, 2042, 2099
RseI CAYNNNNRTG 2 cut(s) 514, 828
SalI GTCGAC 1 cut(s) 1989
SapI GCTCTTC 1 cut(s) 1420
SaqAI TTAA 7 cut(s) 1268, 1311, 1484, 1661, 1976, 2010, 2124
Sau3AI GATC 9 cut(s) 10, 43, 349, 478, 499, 524, 733, 1228, 2064
Sau96I GGNCC 2 cut(s) 179, 905
ScaI AGTACT 1 cut(s) 2100
SchI GAGTC 3 cut(s) 248, 734, 2202
ScrFI CCNGG 4 cut(s) 1259, 1773, 1825, 2255
SduI GDGCHC 2 cut(s) 1700, 2215
SfaNI GCATC 9 cut(s) 79, 193, 560, 602, 971, 979, 1063, 1643, 1837
SfcI CTRYAG 7 cut(s) 76, 303, 897, 1101, 1563, 1572, 2068
Sfr274I CTCGAG 1 cut(s) 108
SinI GGWCC 1 cut(s) 905
SlaI CTCGAG 1 cut(s) 108
SmiMI CAYNNNNRTG 2 cut(s) 514, 828
SmlI CTYRAG 3 cut(s) 108, 1877, 2060
SmoI CTYRAG 3 cut(s) 108, 1877, 2060
SphI GCATGC 2 cut(s) 1746, 1750
Sse9I AATT 9 cut(s) 99, 366, 459, 1184, 1357, 1439, 1542, 1806, 1977
SsiI CCGC 4 cut(s) 35, 1296, 1476, 1657
SspMI CTAG 6 cut(s) 126, 465, 584, 828, 1803, 2106
StyD4I CCNGG 4 cut(s) 1257, 1771, 1823, 2253
StyI CCWWGG 3 cut(s) 1029, 1996, 2175
TaiI ACGT 5 cut(s) 56, 214, 532, 822, 1964
TaqI TCGA 4 cut(s) 109, 541, 1943, 1990
TasI AATT 9 cut(s) 99, 366, 459, 1184, 1357, 1439, 1542, 1806, 1977
TatI WGTACW 1 cut(s) 2098
TauI GCSGC 2 cut(s) 1478, 1659
TfiI GAWTC 7 cut(s) 749, 983, 1460, 1530, 1799, 1945, 2033
Tru1I TTAA 7 cut(s) 1268, 1311, 1484, 1661, 1976, 2010, 2124
Tru9I TTAA 7 cut(s) 1268, 1311, 1484, 1661, 1976, 2010, 2124
TscAI CASTG 7 cut(s) 80, 181, 361, 687, 1560, 1594, 2059
TseFI GTSAC 2 cut(s) 240, 888
Tsp45I GTSAC 2 cut(s) 240, 888
TspDTI ATGAA 7 cut(s) 17, 213, 554, 1104, 1134, 1521, 2129
TspGWI ACGGA 2 cut(s) 696, 2133
TspRI CASTG 7 cut(s) 80, 181, 361, 687, 1560, 1594, 2059
VpaK11BI GGWCC 1 cut(s) 905
VspI ATTAAT 1 cut(s) 1976
XapI RAATTY 1 cut(s) 99
XbaI TCTAGA 1 cut(s) 1802
XceI RCATGY 4 cut(s) 938, 955, 1746, 1750
XhoI CTCGAG 1 cut(s) 108
XmiI GTMKAC 1 cut(s) 1990
XmnI GAANNNNTTC 1 cut(s) 1534
XspI CTAG 6 cut(s) 126, 465, 584, 828, 1803, 2106
ZrmI AGTACT 1 cut(s) 2100
Zsp2I ATGCAT 1 cut(s) 1748
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.