Rroxscaffold_5G00340990

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001434
Physical Location & Seq
Reverse (-)
9467637 .. 9476016
8380 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00340990.1

Sequence Viewer

Length: 2103 bp
ATGAGGGCAGGTTCGTTGTGGGCCGACGAGCCCAATTCTCCTTCTACCTCCGTGCCCCGCGTTCCCCAGCCTAATCCCTCCAAGCACAGTGTCTTTGATGTTACTGGCAGTGTTAGCAATGTGTTTAACTTGTTAGCACGGAGAGAGGTCTCTCCTCGAACAAAACATTCCCCGAAGAAGTTATGGGGGAAAGCTTCCAAGAGTCAGTCTGATAACATTGGCCCAAGGTGTGAAGCAGCTAGAGATGCTAGAAATGGCCTTTTGTCATGGGTGGAGGCAGAGTCGCTCCAGCATTTATCTGCAAAATATTGCCCGCTTGTGCCTCCTCCACGGTCAACTATTGCAGCAGCCTTCAGCCCTGATGGAAAGACACTTGCTTCCACTCATGGAGATCATACTGTAAAGATCATTGATTGCCGAACTGGAAGATGCTTAAAGGTGTTGAGTGGTCACCGCAGGACACCTTGGGTGGTCAGGTTTCATCCTTTGCATCCAGATATACTGGCTAGTGGAAGTCTGGATCATGAAGTGAGTGTTCTTTTCCTCCAAACAGTACGCCCCATTGCTTCCATAGCTTTCCATGCCCAAGGCGATCTTCTTGCTGTTGCATCAGGTCACAAGTTATACATATGGCACTACAATAGAAGAGGAGAGTCATCCTCACCAACAATAGTACTGAAGACACGACGTTCGCTTCGGGCTGTGCATTTTCATCCTCATGCTGCTCCTTTCCTTTTAACTGCTGAGGTCAATGACCTTGACTCTCATGCTCATTCCAGTGATCGTACTAGTTTGGCAGATGGACTACCTCTTATGTCTTTACCATTCCTGATATGGCCTTCATTTGCTAGAGATAGTGGGAGAATATCTATGCAGCGTAGTGATGTGGATATTGGTTCAAGTAGCGCACAACAGAGAGTTGATCCTTCTGCTTCTGTGCGGCTCCTAACATATTCAACTCCATCAGGGCACCTATGGAAGTGCAACCAGAAAGCAGAAGTAATCATATTTTCCCCTTTTGGTGACTCAACATATTGGGAACTTCCTTTCTTGCAAGGGTGGCTAATTGGTCAGACCCAAGCTAGCCAACGGAATATGCGGCTGGTAAGTGATTCTGCCCATGACAATCCATCTACAAATGGTGAGATGGACAGCCGAGCTCCTATCACTTCCTCAGTAATACCGACCGGTGTAAACCAACCTAGGGTTACTGGAAGATCTAGTTCCCGGCACCGTACTTCACGTTCTCATATGTTGTCAGCAATTGGGTCTAATGAGGGTGCTGGATTCAATAATATTGCTCATGGTGAAAGTGAGCCTCAACCTGTTGTCAGCAGAATCCAATCTGAGCTTGCTAACTCCCTTGCTGCAGCAGCGGCGGCAGAGTTACCTTGTACTGTGAAGCTCAGAGTTTGGCCACATGATGTGAAAAATCCGTGTGCTCCCCTTGATGCTGAAAGATGTTACTTAACTATTCCGCATGCCGTACTTTGTAGTGAAATGGGAGCCCATTTTTCACCTTGTGGAAGGTTTTTAGCAGCCTGTGTTGCATGTATGTTGCCTCATTTGGAAGGTGATCCTGGATTACAGGGCCAGGCCAACCATGATATCACAGGGGCAGCAACCTCCCCAACTCGTCACCCAATTTCGGCTCACCATGTCGTGTATGAGCTCCGGATATATTCCCTCGAGGAGGCAACGTTTGGCATGGTTCTTGCATCCCGGGCAATACGAGCTGCCCATTGCTTGACATCTATTCAGTTCTCTCCTACGTCAGAACACATATTACTTGCGTATGGCCGGCGTCACAGTTCACTTCTTAAGAGTGTTGTCATTGACGGAGAGACAACAGTGCCTATTTACACCATTCTGGAGGTATATAGAGTTTCTGATATGGAACTTGTGAGAATTCTCCCTAGTGCAGAAGATGAGGTCAACGTAGCTTGTTTTCATCCTTCTGTCGGAGGTGGCCTTGTTTATGGAACTAAGGAAGGGAAGTTAAGGATCCTCCAATATGATAGTTCTCATGCTGTGGGTCATACGACTTCCAGTTTTCTTGATGAAAACATGCTAGAGGTCCCGACTTATGCTTTAGAATGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04137 ko04140 map04137 map04140
Pfam Domains
Protein Families
WD40

Protein Analysis

700

Amino Acids

76.3

Weight (kDa)

8.15

Isoelectric Point (pI)

49.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 116 - 178 9.9e-10 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 116 - 179 1.8e-07 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 117 - 251 4.1e-12 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 117 - 196 9.1e-06 CDC20/Fizzy WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 1688
AccB1I GGYRCC 2 cut(s) 969, 1230
AccII CGCG 1 cut(s) 60
AccIII TCCGGA 1 cut(s) 1674
AciI CCGC 8 cut(s) 58, 314, 454, 940, 1099, 1376, 1379, 1478
AclI AACGTT 1 cut(s) 1700
AclWI GGATC 5 cut(s) 528, 917, 1571, 1999, 2012
AcoI YGGCCR 2 cut(s) 1415, 1798
AcsI RAATTY 1 cut(s) 1908
AcuI CTGAAG 2 cut(s) 337, 698
AcyI GRCGYC 1 cut(s) 1804
AdeI CACNNNGTG 1 cut(s) 1523
AfaI GTAC 6 cut(s) 555, 675, 787, 1237, 1396, 1488
AfiI CCNNNNNNNGG 4 cut(s) 1204, 1648, 1693, 1961
AflII CTTAAG 1 cut(s) 1820
AgeI ACCGGT 1 cut(s) 1187
AgsI TTSAA 3 cut(s) 900, 957, 1291
AhlI ACTAGT 1 cut(s) 788
AjnI CCWGG 2 cut(s) 1579, 1593
AjuI GAANNNNNNNTTGG 2 cut(s) 579, 611
Alw21I GWGCWC 3 cut(s) 1162, 1444, 1674
Alw26I GTCTC 2 cut(s) 154, 1838
AlwI GGATC 5 cut(s) 528, 917, 1571, 1999, 2012
Ama87I CYCGRG 2 cut(s) 1688, 1722
Aor13HI TCCGGA 1 cut(s) 1674
AoxI GGCC 9 cut(s) 21, 220, 256, 836, 1415, 1591, 1596, 1798, 1969
ApoI RAATTY 1 cut(s) 1908
ArsI GACNNNNNNTTYG 4 cut(s) 673, 678, 705, 710
AsiGI ACCGGT 1 cut(s) 1187
AspA2I CCTAGG 1 cut(s) 1202
AspLEI GCGC 1 cut(s) 908
AspS9I GGNCC 4 cut(s) 21, 221, 1591, 2077
AsuC2I CCSGG 3 cut(s) 1228, 1723, 1724
AsuHPI GGTGA 9 cut(s) 443, 654, 1034, 1154, 1319, 1509, 1586, 1631, 1646
AsuNHI GCTAGC 1 cut(s) 1082
AvaI CYCGRG 2 cut(s) 1688, 1722
AvaII GGWCC 1 cut(s) 2077
AvrII CCTAGG 1 cut(s) 1202
BaeGI GKGCMC 2 cut(s) 57, 972
BalI TGGCCA 1 cut(s) 1417
BamHI GGATCC 1 cut(s) 2004
BanI GGYRCC 2 cut(s) 969, 1230
BanII GRGCYC 4 cut(s) 33, 1162, 1510, 1674
BbsI GAAGAC 1 cut(s) 686
Bbv12I GWGCWC 3 cut(s) 1162, 1444, 1674
BbvCI CCTCAGC 1 cut(s) 744
BccI CCATC 5 cut(s) 356, 794, 970, 1138, 1141
BceAI ACGGC 1 cut(s) 1469
BcgI CGANNNNNNTGC 2 cut(s) 581, 615
BciT130I CCWGG 2 cut(s) 1581, 1595
BcnI CCSGG 3 cut(s) 1228, 1723, 1724
BcoDI GTCTC 2 cut(s) 154, 1838
BcuI ACTAGT 1 cut(s) 788
BfmI CTRYAG 1 cut(s) 1368
BfrI CTTAAG 1 cut(s) 1820
BglII AGATCT 1 cut(s) 1217
BlnI CCTAGG 1 cut(s) 1202
BmcAI AGTACT 1 cut(s) 675
Bme1390I CCNGG 5 cut(s) 1228, 1581, 1595, 1723, 1724
Bme18I GGWCC 1 cut(s) 2077
BmeT110I CYCGRG 2 cut(s) 1688, 1722
BmgT120I GGNCC 4 cut(s) 21, 221, 1591, 2077
BmiI GGNNCC 6 cut(s) 944, 971, 1232, 1507, 2006, 2079
BmrFI CCNGG 5 cut(s) 1228, 1581, 1595, 1723, 1724
BmsI GCATC 6 cut(s) 235, 419, 499, 617, 1441, 1727
BmtI GCTAGC 1 cut(s) 1086
BpiI GAAGAC 1 cut(s) 686
BplI GAGNNNNNCTC 2 cut(s) 644, 676
BpmI CTGGAG 2 cut(s) 272, 1892
Bpu10I CCTNAGC 1 cut(s) 744
BpuMI CCSGG 3 cut(s) 1228, 1723, 1724
BsaHI GRCGYC 1 cut(s) 1804
BsaI GGTCTC 1 cut(s) 154
BsaJI CCNNGG 6 cut(s) 224, 329, 464, 586, 1202, 1722
BsaWI WCCGGW 2 cut(s) 1187, 1674
BsaXI ACNNNNNCTCC 4 cut(s) 266, 296, 1685, 1715
Bsc4I CCNNNNNNNGG 4 cut(s) 1204, 1648, 1693, 1961
Bse118I RCCGGY 2 cut(s) 1187, 1800
Bse1I ACTGG 6 cut(s) 109, 427, 507, 777, 1216, 2049
Bse3DI GCAATG 3 cut(s) 124, 561, 1741
BseAI TCCGGA 1 cut(s) 1674
BseBI CCWGG 2 cut(s) 1581, 1595
BseDI CCNNGG 6 cut(s) 224, 329, 464, 586, 1202, 1722
BseGI GGATG 6 cut(s) 481, 490, 656, 712, 1718, 1951
BseLI CCNNNNNNNGG 4 cut(s) 1204, 1648, 1693, 1961
BseMI GCAATG 3 cut(s) 124, 561, 1741
BseMII CTCAG 4 cut(s) 735, 1188, 1338, 1420
BseNI ACTGG 6 cut(s) 109, 427, 507, 777, 1216, 2049
BseRI GAGGAG 4 cut(s) 144, 315, 663, 1706
BseSI GKGCMC 2 cut(s) 57, 972
BseYI CCCAGC 1 cut(s) 66
BsgI GTGCAG 1 cut(s) 1941
Bsh1236I CGCG 1 cut(s) 60
Bsh1285I CGRYCG 1 cut(s) 1188
BshFI GGCC 9 cut(s) 23, 222, 258, 838, 1417, 1593, 1598, 1800, 1971
BshNI GGYRCC 2 cut(s) 969, 1230
BshTI ACCGGT 1 cut(s) 1187
BsiEI CGRYCG 1 cut(s) 1188
BsiHKAI GWGCWC 3 cut(s) 1162, 1444, 1674
BsiHKCI CYCGRG 2 cut(s) 1688, 1722
BsiSI CCGG 5 cut(s) 1188, 1228, 1675, 1723, 1801
BslFI GGGAC 1 cut(s) 2063
BslI CCNNNNNNNGG 4 cut(s) 1204, 1648, 1693, 1961
BsmAI GTCTC 2 cut(s) 154, 1838
BsmFI GGGAC 1 cut(s) 2063
BsmI GAATGC 1 cut(s) 2102
BsnI GGCC 9 cut(s) 23, 222, 258, 838, 1417, 1593, 1598, 1800, 1971
Bso31I GGTCTC 1 cut(s) 154
BsoBI CYCGRG 2 cut(s) 1688, 1722
Bsp1286I GDGCHC 7 cut(s) 33, 57, 972, 1162, 1444, 1510, 1674
Bsp13I TCCGGA 1 cut(s) 1674
Bsp143I GATC 9 cut(s) 391, 405, 520, 592, 781, 922, 1217, 1576, 2004
BspACI CCGC 8 cut(s) 58, 314, 454, 940, 1099, 1376, 1379, 1478
BspANI GGCC 9 cut(s) 23, 222, 258, 838, 1417, 1593, 1598, 1800, 1971
BspCNI CTCAG 4 cut(s) 736, 1187, 1339, 1419
BspEI TCCGGA 1 cut(s) 1674
BspFNI CGCG 1 cut(s) 60
BspHI TCATGA 1 cut(s) 523
BspLI GGNNCC 6 cut(s) 944, 971, 1232, 1507, 2006, 2079
BspMAI CTGCAG 1 cut(s) 1372
BspOI GCTAGC 1 cut(s) 1086
BspPI GGATC 5 cut(s) 528, 917, 1571, 1999, 2012
BspT107I GGYRCC 2 cut(s) 969, 1230
BspTI CTTAAG 1 cut(s) 1820
BspTNI GGTCTC 1 cut(s) 154
BsrDI GCAATG 3 cut(s) 124, 561, 1741
BsrFI RCCGGY 2 cut(s) 1187, 1800
BsrI ACTGG 6 cut(s) 109, 427, 507, 777, 1216, 2049
BssAI RCCGGY 2 cut(s) 1187, 1800
BssECI CCNNGG 6 cut(s) 224, 329, 464, 586, 1202, 1722
BssMI GATC 9 cut(s) 391, 405, 520, 592, 781, 922, 1217, 1576, 2004
BssNI GRCGYC 1 cut(s) 1804
BssT1I CCWWGG 4 cut(s) 224, 464, 586, 1202
Bst2UI CCWGG 2 cut(s) 1581, 1595
Bst4CI ACNGT 8 cut(s) 89, 333, 400, 553, 1235, 1399, 1811, 1852
Bst6I CTCTTC 1 cut(s) 640
BstACI GRCGYC 1 cut(s) 1804
BstAFI CTTAAG 1 cut(s) 1820
BstC8I GCNNGC 5 cut(s) 314, 1084, 1353, 1482, 1802
BstDEI CTNAG 5 cut(s) 744, 1174, 1347, 1406, 1986
BstDSI CCRYGG 1 cut(s) 329
BstEII GGTNACC 1 cut(s) 449
BstENI CCTNNNNNAGG 1 cut(s) 1691
BstF5I GGATG 6 cut(s) 481, 490, 656, 712, 1718, 1951
BstFNI CGCG 1 cut(s) 60
BstHHI GCGC 1 cut(s) 908
BstKTI GATC 9 cut(s) 394, 408, 523, 595, 784, 925, 1220, 1579, 2007
BstMAI GTCTC 2 cut(s) 154, 1838
BstMBI GATC 9 cut(s) 391, 405, 520, 592, 781, 922, 1217, 1576, 2004
BstMCI CGRYCG 1 cut(s) 1188
BstNI CCWGG 2 cut(s) 1581, 1595
BstNSI RCATGY 3 cut(s) 1484, 1554, 2071
BstPI GGTNACC 1 cut(s) 449
BstSCI CCNGG 5 cut(s) 1226, 1579, 1593, 1721, 1722
BstSFI CTRYAG 1 cut(s) 1368
BstSLI GKGCMC 2 cut(s) 57, 972
BstUI CGCG 1 cut(s) 60
BstV2I GAAGAC 1 cut(s) 686
BstX2I RGATCY 2 cut(s) 1217, 2004
BstYI RGATCY 2 cut(s) 1217, 2004
BsuRI GGCC 9 cut(s) 23, 222, 258, 838, 1417, 1593, 1598, 1800, 1971
BtgI CCRYGG 1 cut(s) 329
BtsCI GGATG 6 cut(s) 481, 490, 656, 712, 1718, 1951
BtsI GCAGTG 1 cut(s) 115
BtsIMutI CAGTG 4 cut(s) 94, 115, 784, 1857
Cac8I GCNNGC 5 cut(s) 314, 1084, 1353, 1482, 1802
CciI TCATGA 1 cut(s) 523
CfoI GCGC 1 cut(s) 908
Cfr10I RCCGGY 2 cut(s) 1187, 1800
Cfr13I GGNCC 4 cut(s) 21, 221, 1591, 2077
Cfr9I CCCGGG 1 cut(s) 1722
CseI GACGC 1 cut(s) 1793
Csp6I GTAC 6 cut(s) 554, 674, 786, 1236, 1395, 1487
CspAI ACCGGT 1 cut(s) 1187
CviQI GTAC 6 cut(s) 554, 674, 786, 1236, 1395, 1487
DdeI CTNAG 5 cut(s) 744, 1174, 1347, 1406, 1986
DpnI GATC 9 cut(s) 393, 407, 522, 594, 783, 924, 1219, 1578, 2006
DpnII GATC 9 cut(s) 391, 405, 520, 592, 781, 922, 1217, 1576, 2004
DraIII CACNNNGTG 1 cut(s) 1523
EaeI YGGCCR 2 cut(s) 1415, 1798
Eam1104I CTCTTC 1 cut(s) 640
EarI CTCTTC 1 cut(s) 640
Ecl136II GAGCTC 2 cut(s) 1160, 1672
Eco130I CCWWGG 4 cut(s) 224, 464, 586, 1202
Eco24I GRGCYC 4 cut(s) 33, 1162, 1510, 1674
Eco31I GGTCTC 1 cut(s) 154
Eco32I GATATC 1 cut(s) 1609
Eco47I GGWCC 1 cut(s) 2077
Eco53kI GAGCTC 2 cut(s) 1160, 1672
Eco57I CTGAAG 2 cut(s) 337, 698
Eco88I CYCGRG 2 cut(s) 1688, 1722
Eco91I GGTNACC 1 cut(s) 449
EcoICRI GAGCTC 2 cut(s) 1160, 1672
EcoNI CCTNNNNNAGG 1 cut(s) 1691
EcoO109I RGGNCCY 1 cut(s) 2077
EcoO65I GGTNACC 1 cut(s) 449
EcoRI GAATTC 1 cut(s) 1908
EcoRII CCWGG 2 cut(s) 1579, 1593
EcoRV GATATC 1 cut(s) 1609
EcoT14I CCWWGG 4 cut(s) 224, 464, 586, 1202
EcoT38I GRGCYC 4 cut(s) 33, 1162, 1510, 1674
ErhI CCWWGG 4 cut(s) 224, 464, 586, 1202
FalI AAGNNNNNCTT 2 cut(s) 579, 611
FaqI GGGAC 1 cut(s) 2063
FauI CCCGC 2 cut(s) 65, 321
FauNDI CATATG 2 cut(s) 629, 1251
FokI GGATG 6 cut(s) 468, 477, 643, 699, 1705, 1938
FriOI GRGCYC 4 cut(s) 33, 1162, 1510, 1674
GlaI GCGC 1 cut(s) 907
GsaI CCCAGC 1 cut(s) 70
GsuI CTGGAG 2 cut(s) 272, 1892
HaeIII GGCC 9 cut(s) 23, 222, 258, 838, 1417, 1593, 1598, 1800, 1971
HapII CCGG 5 cut(s) 1188, 1228, 1675, 1723, 1801
HgaI GACGC 1 cut(s) 1793
HhaI GCGC 1 cut(s) 908
Hin1I GRCGYC 1 cut(s) 1804
Hin6I GCGC 1 cut(s) 906
HinP1I GCGC 1 cut(s) 906
HincII GTYRAC 2 cut(s) 336, 1936
HindII GTYRAC 2 cut(s) 336, 1936
HindIII AAGCTT 1 cut(s) 192
HinfI GANTC 8 cut(s) 202, 281, 653, 761, 1025, 1112, 1287, 1338
HpaII CCGG 5 cut(s) 1188, 1228, 1675, 1723, 1801
HphI GGTGA 9 cut(s) 443, 654, 1034, 1154, 1319, 1509, 1586, 1631, 1646
Hpy166II GTNNAC 4 cut(s) 336, 1195, 1814, 1936
Hpy188I TCNGA 7 cut(s) 211, 1074, 1348, 1409, 1777, 1891, 1964
Hpy188III TCNNGA 8 cut(s) 494, 518, 524, 829, 1675, 1871, 2057, 2080
Hpy8I GTNNAC 4 cut(s) 336, 1195, 1814, 1936
Hpy99I CGWCG 2 cut(s) 29, 690
HpyAV CCTTC 8 cut(s) 51, 361, 849, 936, 1521, 1565, 1965, 1985
HpyCH4III ACNGT 8 cut(s) 89, 333, 400, 553, 1235, 1399, 1811, 1852
HpyCH4IV ACGT 5 cut(s) 688, 1243, 1700, 1772, 1938
HpyF3I CTNAG 5 cut(s) 744, 1174, 1347, 1406, 1986
HpySE526I ACGT 5 cut(s) 688, 1243, 1700, 1772, 1938
Hsp92I GRCGYC 1 cut(s) 1804
HspAI GCGC 1 cut(s) 906
Kpn2I TCCGGA 1 cut(s) 1674
KroI GCCGGC 1 cut(s) 1800
KroNI GCCGGC 1 cut(s) 1802
Kzo9I GATC 9 cut(s) 391, 405, 520, 592, 781, 922, 1217, 1576, 2004
LmnI GCTCC 7 cut(s) 291, 730, 948, 1165, 1447, 1505, 1677
LweI GCATC 6 cut(s) 235, 419, 499, 617, 1441, 1727
MaeII ACGT 5 cut(s) 688, 1243, 1700, 1772, 1938
MaeIII GTNAC 9 cut(s) 100, 449, 614, 1022, 1207, 1386, 1463, 1637, 1805
MalI GATC 9 cut(s) 393, 407, 522, 594, 783, 924, 1219, 1578, 2006
MboI GATC 9 cut(s) 391, 405, 520, 592, 781, 922, 1217, 1576, 2004
MboII GAAGA 7 cut(s) 187, 438, 587, 657, 691, 1227, 1937
MfeI CAATTG 1 cut(s) 1263
MflI RGATCY 2 cut(s) 1217, 2004
MhlI GDGCHC 7 cut(s) 33, 57, 972, 1162, 1444, 1510, 1674
MlsI TGGCCA 1 cut(s) 1417
MluCI AATT 5 cut(s) 34, 1065, 1263, 1644, 1908
MluNI TGGCCA 1 cut(s) 1417
MlyI GAGTC 5 cut(s) 211, 290, 662, 755, 1019
MmeI TCCRAC 1 cut(s) 1942
Mox20I TGGCCA 1 cut(s) 1417
MroI TCCGGA 1 cut(s) 1674
MroNI GCCGGC 1 cut(s) 1800
MscI TGGCCA 1 cut(s) 1417
MseI TTAA 6 cut(s) 126, 434, 737, 1469, 1821, 2000
MslI CAYNNNNRTG 2 cut(s) 717, 777
Msp20I TGGCCA 1 cut(s) 1417
MspA1I CMGCKG 1 cut(s) 1376
MspCI CTTAAG 1 cut(s) 1820
MspI CCGG 5 cut(s) 1188, 1228, 1675, 1723, 1801
MspR9I CCNGG 5 cut(s) 1228, 1581, 1595, 1723, 1724
MunI CAATTG 1 cut(s) 1263
Mva1269I GAATGC 1 cut(s) 2102
MvaI CCWGG 2 cut(s) 1581, 1595
MvnI CGCG 1 cut(s) 60
NaeI GCCGGC 1 cut(s) 1802
NciI CCSGG 3 cut(s) 1228, 1723, 1724
NdeI CATATG 2 cut(s) 629, 1251
NdeII GATC 9 cut(s) 391, 405, 520, 592, 781, 922, 1217, 1576, 2004
NgoMIV GCCGGC 1 cut(s) 1800
NheI GCTAGC 1 cut(s) 1082
NlaIV GGNNCC 6 cut(s) 944, 971, 1232, 1507, 2006, 2079
NmeAIII GCCGAG 1 cut(s) 1181
NmuCI GTSAC 5 cut(s) 449, 614, 1022, 1637, 1805
NspI RCATGY 3 cut(s) 1484, 1554, 2071
PaeI GCATGC 1 cut(s) 1484
PaeR7I CTCGAG 1 cut(s) 1688
PagI TCATGA 1 cut(s) 523
PctI GAATGC 1 cut(s) 2102
PdiI GCCGGC 1 cut(s) 1802
PfeI GAWTC 3 cut(s) 1112, 1287, 1338
PfoI TCCNGGA 1 cut(s) 1579
PinAI ACCGGT 1 cut(s) 1187
PleI GAGTC 5 cut(s) 210, 289, 661, 755, 1019
PpsI GAGTC 5 cut(s) 210, 289, 661, 755, 1019
PpuMI RGGWCCY 1 cut(s) 2077
Psp124BI GAGCTC 2 cut(s) 1162, 1674
Psp1406I AACGTT 1 cut(s) 1700
Psp5II RGGWCCY 1 cut(s) 2077
Psp6I CCWGG 2 cut(s) 1579, 1593
PspEI GGTNACC 1 cut(s) 449
PspFI CCCAGC 1 cut(s) 66
PspGI CCWGG 2 cut(s) 1579, 1593
PspN4I GGNNCC 6 cut(s) 944, 971, 1232, 1507, 2006, 2079
PspPI GGNCC 4 cut(s) 21, 221, 1591, 2077
PspPPI RGGWCCY 1 cut(s) 2077
PspXI VCTCGAGB 1 cut(s) 1688
PsrI GAACNNNNNNTAC 4 cut(s) 1228, 1260, 1770, 1802
PstI CTGCAG 1 cut(s) 1372
PsuI RGATCY 2 cut(s) 1217, 2004
RsaI GTAC 6 cut(s) 555, 675, 787, 1237, 1396, 1488
RsaNI GTAC 6 cut(s) 554, 674, 786, 1236, 1395, 1487
RseI CAYNNNNRTG 2 cut(s) 717, 777
SacI GAGCTC 2 cut(s) 1162, 1674
SaqAI TTAA 6 cut(s) 126, 434, 737, 1469, 1821, 2000
Sau3AI GATC 9 cut(s) 391, 405, 520, 592, 781, 922, 1217, 1576, 2004
Sau96I GGNCC 4 cut(s) 21, 221, 1591, 2077
ScaI AGTACT 1 cut(s) 675
SchI GAGTC 5 cut(s) 211, 290, 662, 755, 1019
ScrFI CCNGG 5 cut(s) 1228, 1581, 1595, 1723, 1724
SduI GDGCHC 7 cut(s) 33, 57, 972, 1162, 1444, 1510, 1674
SfaNI GCATC 6 cut(s) 235, 419, 499, 617, 1441, 1727
SfcI CTRYAG 1 cut(s) 1368
Sfr274I CTCGAG 1 cut(s) 1688
SinI GGWCC 1 cut(s) 2077
SlaI CTCGAG 1 cut(s) 1688
SmaI CCCGGG 1 cut(s) 1724
SmiMI CAYNNNNRTG 2 cut(s) 717, 777
SmlI CTYRAG 2 cut(s) 1688, 1820
SmoI CTYRAG 2 cut(s) 1688, 1820
SpeI ACTAGT 1 cut(s) 788
SphI GCATGC 1 cut(s) 1484
Sse9I AATT 5 cut(s) 34, 1065, 1263, 1644, 1908
SsiI CCGC 8 cut(s) 58, 314, 454, 940, 1099, 1376, 1379, 1478
SspI AATATT 2 cut(s) 308, 1297
SstI GAGCTC 2 cut(s) 1162, 1674
StyD4I CCNGG 5 cut(s) 1226, 1579, 1593, 1721, 1722
StyI CCWWGG 4 cut(s) 224, 464, 586, 1202
TaaI ACNGT 8 cut(s) 89, 333, 400, 553, 1235, 1399, 1811, 1852
TaiI ACGT 5 cut(s) 691, 1246, 1703, 1775, 1941
TaqI TCGA 2 cut(s) 157, 1689
TasI AATT 5 cut(s) 34, 1065, 1263, 1644, 1908
TatI WGTACW 2 cut(s) 673, 1394
TauI GCSGC 4 cut(s) 943, 1102, 1379, 1382
TfiI GAWTC 3 cut(s) 1112, 1287, 1338
Tru1I TTAA 6 cut(s) 126, 434, 737, 1469, 1821, 2000
Tru9I TTAA 6 cut(s) 126, 434, 737, 1469, 1821, 2000
TscAI CASTG 4 cut(s) 94, 115, 784, 1857
TseFI GTSAC 5 cut(s) 449, 614, 1022, 1637, 1805
Tsp45I GTSAC 5 cut(s) 449, 614, 1022, 1637, 1805
TspDTI ATGAA 6 cut(s) 470, 540, 701, 831, 1940, 2076
TspGWI ACGGA 5 cut(s) 40, 154, 1105, 1425, 1854
TspMI CCCGGG 1 cut(s) 1722
TspRI CASTG 4 cut(s) 94, 115, 784, 1857
Vha464I CTTAAG 1 cut(s) 1820
VpaK11BI GGWCC 1 cut(s) 2077
XagI CCTNNNNNAGG 1 cut(s) 1691
XapI RAATTY 1 cut(s) 1908
XceI RCATGY 3 cut(s) 1484, 1554, 2071
XcmI CCANNNNNNNNNTGG 1 cut(s) 831
XhoI CTCGAG 1 cut(s) 1688
XmaI CCCGGG 1 cut(s) 1722
XmaJI CCTAGG 1 cut(s) 1202
ZrmI AGTACT 1 cut(s) 675
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.