Rorug03G0351800

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001434
Physical Location & Seq
Forward (+)
43021152 .. 43025346
4195 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0351800.1

Sequence Viewer

Length: 912 bp
ATGGGTTTTAAATCTGCAATCTTGTTGTTGTTATATATCACTATCACAATCACAATCACAACGCTACAATTGGTATCGTCGTCTTCGCCTGTCTGCCCTCCCCAGTCCGATCTGTTCCGTATCACTCTTCAATCTCAATGCCCCGTCGCAACCTCCCCCTACCCACCCCTCCAGGTGGATGGGGACTTCATTGACAGAGCATTAGCATCAACACGGAAGACTGGTTATACTTCTGTGCTCTTTTACGCTTCCTGGTGCCCATTTTCACGCAAATTGTATCCTATTTTTGAAATGCTTAGCTTCATGTTCCCTCAAGTGGAGCATTTAGCAGTTGAGCAATCTTCAGCCTTCCCAAGTGTATTCTCAAGATATGGAATCCCTAGCTTCCCTTCTATTTTAATAGTCAATCAGACGTCAAGGGTCCGATATTATGGTCCAAAAGATCTCCTGTCCCTTGTCCAATTTTACCAGAAAACCACAGGACTTGAGCCGGTTCAGTATTACAATCATGATCAATCCGTCAGTTGTGAATCTGGTGAAAAATCCATAATCAAATCAATGAGTAACCTGCTGTCACTAAGAGAGATATCAAGGAGGGAACCCTATTTAGTTTTCGCTACATTGTTTCTCTGTCTTAGGGCACTTATACATATATTGCCTAAGGTATTGACTCACCTGAAAGCTTTCTGGGTTCTATACGTTCCACATTTCAACGTGGGAATATTTGGGGAGACGAGTCAAACTATGGGGCGTATCCTTCACATGGTTGATGTTAGGAGGATTTGGAACAAGCTGAGATTATGCAAGACCAGGAATTTCCACGAAGGTGCAAAGAATGCCAGGGTTTGGGCTTCTTCCCTGACTTCAGTCTCTTTGGGTGAATCTTCATCGGCTAGATCATCGTCATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04137 ko04140 map04137 map04140
Pfam Domains
Protein Families
WD40

Protein Analysis

303

Amino Acids

34.26

Weight (kDa)

9.53

Isoelectric Point (pI)

57.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 79 - 156 3.3e-06 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 416
Acc36I ACCTGC 1 cut(s) 576
AccB1I GGYRCC 1 cut(s) 255
AccB7I CCANNNNNTGG 1 cut(s) 846
AcsI RAATTY 1 cut(s) 814
AcuI CTGAAG 2 cut(s) 327, 849
AcyI GRCGYC 1 cut(s) 413
AfiI CCNNNNNNNGG 4 cut(s) 175, 316, 763, 846
AgsI TTSAA 3 cut(s) 131, 290, 712
AjnI CCWGG 4 cut(s) 171, 251, 809, 839
AleI CACNNNNGTG 1 cut(s) 825
AluBI AGCT 4 cut(s) 300, 384, 683, 793
AluI AGCT 4 cut(s) 300, 384, 683, 793
Alw21I GWGCWC 1 cut(s) 240
Alw26I GTCTC 2 cut(s) 725, 874
ApoI RAATTY 1 cut(s) 814
Asp700I GAANNNNTTC 1 cut(s) 683
AspS9I GGNCC 2 cut(s) 421, 434
AsuHPI GGTGA 3 cut(s) 548, 665, 890
AvaII GGWCC 2 cut(s) 421, 434
AxyI CCTNAGG 1 cut(s) 660
BaeGI GKGCMC 2 cut(s) 260, 643
BanI GGYRCC 1 cut(s) 255
BbsI GAAGAC 2 cut(s) 75, 224
Bbv12I GWGCWC 1 cut(s) 240
BccI CCATC 1 cut(s) 173
BciT130I CCWGG 4 cut(s) 173, 253, 811, 841
BciVI GTATCC 2 cut(s) 288, 764
BclI TGATCA 1 cut(s) 511
BcoDI GTCTC 2 cut(s) 725, 874
BfaI CTAG 2 cut(s) 381, 894
BfuAI ACCTGC 1 cut(s) 576
BfuI GTATCC 2 cut(s) 288, 764
BglII AGATCT 1 cut(s) 442
BlpI GCTNAGC 1 cut(s) 296
Bme1390I CCNGG 4 cut(s) 173, 253, 811, 841
Bme18I GGWCC 2 cut(s) 421, 434
BmgT120I GGNCC 2 cut(s) 421, 434
BmiI GGNNCC 3 cut(s) 257, 422, 600
BmrFI CCNGG 4 cut(s) 173, 253, 811, 841
BmrI ACTGGG 1 cut(s) 97
BmsI GCATC 1 cut(s) 215
BmuI ACTGGG 1 cut(s) 97
BoxI GACNNNNGTC 1 cut(s) 866
BpiI GAAGAC 2 cut(s) 75, 224
BpmI CTGGAG 1 cut(s) 155
Bpu1102I GCTNAGC 1 cut(s) 296
BpuEI CTTGAG 3 cut(s) 297, 349, 506
BsaHI GRCGYC 1 cut(s) 413
BsaJI CCNNGG 1 cut(s) 840
Bsc4I CCNNNNNNNGG 4 cut(s) 175, 316, 763, 846
Bse118I RCCGGY 1 cut(s) 490
Bse1I ACTGG 2 cut(s) 103, 226
Bse21I CCTNAGG 1 cut(s) 660
BseBI CCWGG 4 cut(s) 173, 253, 811, 841
BseDI CCNNGG 1 cut(s) 840
BseGI GGATG 1 cut(s) 184
BseLI CCNNNNNNNGG 4 cut(s) 175, 316, 763, 846
BseMII CTCAG 1 cut(s) 785
BseNI ACTGG 2 cut(s) 103, 226
BseSI GKGCMC 2 cut(s) 260, 643
BshNI GGYRCC 1 cut(s) 255
BsiHKAI GWGCWC 1 cut(s) 240
BsiSI CCGG 1 cut(s) 491
BslFI GGGAC 2 cut(s) 197, 436
BslI CCNNNNNNNGG 4 cut(s) 175, 316, 763, 846
BsmAI GTCTC 2 cut(s) 725, 874
BsmBI CGTCTC 1 cut(s) 725
BsmFI GGGAC 2 cut(s) 197, 436
BsmI GAATGC 1 cut(s) 841
Bsp1286I GDGCHC 3 cut(s) 240, 260, 643
Bsp143I GATC 4 cut(s) 109, 442, 511, 896
Bsp1720I GCTNAGC 1 cut(s) 296
BspCNI CTCAG 1 cut(s) 786
BspHI TCATGA 1 cut(s) 508
BspLI GGNNCC 3 cut(s) 257, 422, 600
BspMI ACCTGC 1 cut(s) 576
BspT107I GGYRCC 1 cut(s) 255
BsrFI RCCGGY 1 cut(s) 490
BsrI ACTGG 2 cut(s) 103, 226
BssAI RCCGGY 1 cut(s) 490
BssECI CCNNGG 1 cut(s) 840
BssMI GATC 4 cut(s) 109, 442, 511, 896
BssNI GRCGYC 1 cut(s) 413
Bst2UI CCWGG 4 cut(s) 173, 253, 811, 841
Bst6I CTCTTC 1 cut(s) 132
BstACI GRCGYC 1 cut(s) 413
BstAPI GCANNNNNTGC 1 cut(s) 836
BstDEI CTNAG 5 cut(s) 296, 578, 635, 660, 794
BstF5I GGATG 1 cut(s) 184
BstKTI GATC 4 cut(s) 112, 445, 514, 899
BstMAI GTCTC 2 cut(s) 725, 874
BstMBI GATC 4 cut(s) 109, 442, 511, 896
BstMWI GCNNNNNNNGC 1 cut(s) 836
BstNI CCWGG 4 cut(s) 173, 253, 811, 841
BstPAI GACNNNNGTC 1 cut(s) 866
BstSCI CCNGG 4 cut(s) 171, 251, 809, 839
BstSLI GKGCMC 2 cut(s) 260, 643
BstV2I GAAGAC 2 cut(s) 75, 224
BstX2I RGATCY 1 cut(s) 442
BstXI CCANNNNNNTGG 1 cut(s) 179
BstYI RGATCY 1 cut(s) 442
Bsu36I CCTNAGG 1 cut(s) 660
BsuI GTATCC 2 cut(s) 288, 764
BtsCI GGATG 1 cut(s) 184
BveI ACCTGC 1 cut(s) 576
CciI TCATGA 1 cut(s) 508
Cfr10I RCCGGY 1 cut(s) 490
Cfr13I GGNCC 2 cut(s) 421, 434
CspCI CAANNNNNGTGG 2 cut(s) 466, 501
CviAII CATG 3 cut(s) 304, 509, 763
CviJI RGCY 8 cut(s) 300, 347, 384, 490, 683, 793, 851, 893
CviKI_1 RGCY 8 cut(s) 300, 347, 384, 490, 683, 793, 851, 893
DdeI CTNAG 5 cut(s) 296, 578, 635, 660, 794
DpnI GATC 4 cut(s) 111, 444, 513, 898
DpnII GATC 4 cut(s) 109, 442, 511, 896
DraI TTTAAA 1 cut(s) 10
Eam1104I CTCTTC 1 cut(s) 132
EarI CTCTTC 1 cut(s) 132
Eco32I GATATC 1 cut(s) 588
Eco47I GGWCC 2 cut(s) 421, 434
Eco57I CTGAAG 2 cut(s) 327, 849
Eco81I CCTNAGG 1 cut(s) 660
EcoRII CCWGG 4 cut(s) 171, 251, 809, 839
EcoRV GATATC 1 cut(s) 588
Esp3I CGTCTC 1 cut(s) 725
FaeI CATG 3 cut(s) 307, 512, 766
FaqI GGGAC 2 cut(s) 197, 436
FatI CATG 3 cut(s) 303, 508, 762
FbaI TGATCA 1 cut(s) 511
FokI GGATG 1 cut(s) 191
FspBI CTAG 2 cut(s) 381, 894
GsuI CTGGAG 1 cut(s) 155
HapII CCGG 1 cut(s) 491
Hin1I GRCGYC 1 cut(s) 413
Hin1II CATG 3 cut(s) 307, 512, 766
HindIII AAGCTT 1 cut(s) 681
HinfI GANTC 5 cut(s) 375, 530, 670, 736, 881
HpaII CCGG 1 cut(s) 491
HphI GGTGA 3 cut(s) 548, 665, 890
Hpy188I TCNGA 3 cut(s) 109, 411, 425
Hpy188III TCNNGA 2 cut(s) 366, 509
Hpy99I CGWCG 2 cut(s) 82, 149
HpyAV CCTTC 4 cut(s) 358, 399, 767, 818
HpyCH4IV ACGT 3 cut(s) 413, 699, 714
HpyCH4V TGCA 3 cut(s) 17, 804, 830
HpyF10VI GCNNNNNNNGC 1 cut(s) 836
HpyF3I CTNAG 5 cut(s) 296, 578, 635, 660, 794
HpySE526I ACGT 3 cut(s) 413, 699, 714
Hsp92I GRCGYC 1 cut(s) 413
Hsp92II CATG 3 cut(s) 307, 512, 766
Ksp22I TGATCA 1 cut(s) 511
Kzo9I GATC 4 cut(s) 109, 442, 511, 896
LmnI GCTCC 1 cut(s) 319
LweI GCATC 1 cut(s) 215
MaeI CTAG 2 cut(s) 381, 894
MaeII ACGT 3 cut(s) 413, 699, 714
MaeIII GTNAC 2 cut(s) 563, 573
MalI GATC 4 cut(s) 111, 444, 513, 898
MboI GATC 4 cut(s) 109, 442, 511, 896
MboII GAAGA 6 cut(s) 75, 119, 229, 333, 846, 876
MfeI CAATTG 1 cut(s) 68
MflI RGATCY 1 cut(s) 442
MhlI GDGCHC 3 cut(s) 240, 260, 643
MluCI AATT 4 cut(s) 68, 272, 461, 814
MlyI GAGTC 2 cut(s) 664, 745
MnlI CCTC 6 cut(s) 108, 163, 179, 321, 588, 771
MroXI GAANNNNTTC 1 cut(s) 683
MseI TTAA 3 cut(s) 9, 398, 910
MslI CAYNNNNRTG 1 cut(s) 825
MspI CCGG 1 cut(s) 491
MspR9I CCNGG 4 cut(s) 173, 253, 811, 841
MunI CAATTG 1 cut(s) 68
Mva1269I GAATGC 1 cut(s) 841
MvaI CCWGG 4 cut(s) 173, 253, 811, 841
MwoI GCNNNNNNNGC 1 cut(s) 836
NdeII GATC 4 cut(s) 109, 442, 511, 896
NlaIII CATG 3 cut(s) 307, 512, 766
NlaIV GGNNCC 3 cut(s) 257, 422, 600
NmuCI GTSAC 1 cut(s) 573
OliI CACNNNNGTG 1 cut(s) 825
PagI TCATGA 1 cut(s) 508
PctI GAATGC 1 cut(s) 841
PdmI GAANNNNTTC 1 cut(s) 683
PfeI GAWTC 3 cut(s) 375, 530, 881
PflMI CCANNNNNTGG 1 cut(s) 846
PleI GAGTC 2 cut(s) 664, 744
PpsI GAGTC 2 cut(s) 664, 744
PshAI GACNNNNGTC 1 cut(s) 866
Psp6I CCWGG 4 cut(s) 171, 251, 809, 839
PspGI CCWGG 4 cut(s) 171, 251, 809, 839
PspN4I GGNNCC 3 cut(s) 257, 422, 600
PspPI GGNCC 2 cut(s) 421, 434
PsuI RGATCY 1 cut(s) 442
RseI CAYNNNNRTG 1 cut(s) 825
SaqAI TTAA 3 cut(s) 9, 398, 910
Sau3AI GATC 4 cut(s) 109, 442, 511, 896
Sau96I GGNCC 2 cut(s) 421, 434
SchI GAGTC 2 cut(s) 664, 745
ScrFI CCNGG 4 cut(s) 173, 253, 811, 841
SduI GDGCHC 3 cut(s) 240, 260, 643
SfaNI GCATC 1 cut(s) 215
SinI GGWCC 2 cut(s) 421, 434
SmiMI CAYNNNNRTG 1 cut(s) 825
SmlI CTYRAG 3 cut(s) 312, 364, 485
SmoI CTYRAG 3 cut(s) 312, 364, 485
Sse9I AATT 4 cut(s) 68, 272, 461, 814
SspI AATATT 1 cut(s) 723
SspMI CTAG 2 cut(s) 381, 894
StyD4I CCNGG 4 cut(s) 171, 251, 809, 839
TaiI ACGT 3 cut(s) 416, 702, 717
TasI AATT 4 cut(s) 68, 272, 461, 814
TfiI GAWTC 3 cut(s) 375, 530, 881
Tru1I TTAA 3 cut(s) 9, 398, 910
Tru9I TTAA 3 cut(s) 9, 398, 910
TseFI GTSAC 1 cut(s) 573
Tsp45I GTSAC 1 cut(s) 573
TspDTI ATGAA 3 cut(s) 178, 292, 876
TspGWI ACGGA 3 cut(s) 107, 229, 508
Van91I CCANNNNNTGG 1 cut(s) 846
VpaK11BI GGWCC 2 cut(s) 421, 434
XapI RAATTY 1 cut(s) 814
XmnI GAANNNNTTC 1 cut(s) 683
XspI CTAG 2 cut(s) 381, 894
ZraI GACGTC 1 cut(s) 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.