Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0001434

Physical Location & Seq

Genomic Coordinates Reverse (-)

23705767 .. 23713027

Gene Structure 7261 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1231400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2298 bp

ATGAGGGCAGGTTCTTCCGACGAGCCCAATTCTCCTTCCACCTCTGTGCCGCGCGTTCCTCAGCCCAATCCGTGCAAGCACAGTGTTAGCAATGTCCTTAGATTGTTAGCACGGAGAGAGGTTTCTCCGCAAACGAAACATTGGTCAAAGAAGTTATGGGGGGAACCTCTGAAGTCTAATTCCGATTCTGTTGGCCCAAAGTTTGAAACAGCTAGAGATGCTAGGCACAGCCTTGTCTCATGGGTGGAGGCAGAGTCACTCCAGCATTTGTCTTCCAAATATTGTCCGCTTGTGCCTCCTCCACGGTCAACTATTGCAGCAGCCTTCAGCCCCGATGGAAAGACACTAGCTTCAACTCACGGAGACCATACTGTAAAGATTATTGATTGCCGAACTGGGATCTGCTTAAAGGTCTTGAGTGGTCACCGTAGGACACCATGGGTGGTTAGATTTCATCCTTTGTATCCAGACATATTGGCTAGTGGAAGTTTGGATCATGAAGTTCGCTTGTGGGATGCAAACACGGCAGAGTGTATAGGATCTCGTGATTTTTTGCGTCCCATTGCATCCATAGCTTTCCATGCCCAAGGTGAGCTGCTCGCTGTTGCGTCAGGTCATAAGTTGTACATATGGCACTACAATGGGAGAGGGGAGACATCCTCACCAAGCATAGTACTGAAGACACGACGTTCGCTCCGGGCTGTGCATTTTCACCCGCATGGAGCTCCTTTCCTTTTAACTGCTGAGGTCAATGACATGGACTCCTCAGATTCCTCGATGACACTTGCAACTTCTCCGGGTTATTTGCGCTATCCTCCACCTACTGTATATTTGGCAGATGGTCAATCTAGTGATCGTTCCAGTTTGGAAGATGGACTACCTCTTATGTCTTTACCATTTCTGATATGGCCTTCATTTGCTAGAGATAATGGGAGAATATCTATGCAGCGTAGTGATGCAGACATTGGTTCAAGTAGTGTGCAACAGAGAGTTGATCCTTCTGCTTCAGTGCGGTTGCTAACATATTCAACTCCTTCAGGGCAGTATGAACTTCTGCTGTCCCCAATTGAACCTAGTAGCTCTTCTCCAGCACCAGAAGAGACAGAAACTAATACTTTCTTGAGTGAAATGGAAACTGAAGTTTCTCAACCTGCAATGGACAGTTTAGAGGCTTTGGAAGTGCAACCTGAAGGGAGGAGCAATCATATTTTCCCATTTGGTGACTCAACATATTGGGAACTTCCTTTTTTGCAAGGGTGGTTAATTGGTCAGACCCAAGCTAGCCAACGGAATATGCGGCTGGTAGGTGATGCTACACAAGATAATCCATCTGCACGTGGTGAGATGGATAATCCAGCCATAACTTCCTCGGTAATACCAACCAGTGTGAGCCAATCCAGGCTTACTGGAAGATCTAGTTCCCGGCATCGTACTTCACGAACTCATATGGTGTCGACAATTGGATCTAATGAAGTTGCTGGATTCTATAGTTTTGCGCATGCTGAAAGTGAGCCTCAACCAGTTGTGAGTAGAATCCAATCTGAACTTGCCAACTCACTTGCTGCAGCAGCAGCTGCAGAGTTGCCTTGCACTGTAAAGCTCAGAATTTGGCCACATGATTTGAAAAGTCCTTGTTCTCCCCTTGATGCAGAAAGATGTCGCTTAACCATTCCACATGCTGTACTTTGTAGTGAGATGGGTGCCCATTTTTCCCCTTGCGGAAGGTTTTTAGCTGCTTGTGTTGCATGTATGTTGCCCCATATGGAAGCTGATCCTGGATTGCAGAGCCAGGTCAACCATGACGCGACAGGGGCATCAACGTCCCCAACTCGACATCCAATTTCAGCACACCATGTTGTGTATGAGCTCCGGATATATTCCCTTGAGGAAGCAACATTTGGAATGGTTCTTGCATCCCGGGCAATAAGAGCTGCCCACTGCTTAACATCTATTCAGTTCTCTCCTACGTCAGAGCATATATTACTTGCCTATGGCCGTCGTCACAGTTCACTTCTTAAGAGTGTTGTCATTGATGGAGAGACAACAGTGCCTATTTACACCATTCTGGAGGTCTACAGAGTTTCTGATATGGAACTTGTGAGAGTTCTTCCTAGTGCAGAGGATGAGGTCAACGTAGCTTCCTTTCATCCTTCAGTTGGAGGTGGCCTTGTCTATGGAACCAAGGAAGGGAAGTTAAGGATCCTCCAATATGATAGTTCTCATTGCATGAATCAGACAACATCTGCTTTTCTTGATGAAAACATGCTTGAGGTCCCGACATATGCCTTGGAATGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04137 ko04140 map04137 map04140

Pfam Domains

Protein Families

WD40

Protein Analysis

766

Amino Acids

83.71

Weight (kDa)

6.15

Isoelectric Point (pI)

56.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_THOC3	PF25174	105 - 241	1.7e-19	THOC3 beta-propeller domain
WD40_Gbeta	PF25391	105 - 202	9.3e-06	G protein beta WD-40 repeat protein
Beta-prop_WDR3_1st	PF25173	106 - 211	7.7e-12	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	108 - 252	2.5e-19	WDR5 beta-propeller domain
WD40_CDC20-Fz	PF24807	108 - 215	3.8e-11	CDC20/Fizzy WD40 domain
WD40_WDHD1_1st	PF24817	108 - 203	5.9e-09	WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	109 - 213	6e-06	WDR36/Utp21 second beta-propeller domain
WD40_Prp19	PF24814	112 - 242	1.6e-10	Prp19 WD40 domain
Beta-prop_EML_2	PF23414	147 - 242	5.6e-06	Echinoderm microtubule-associated protein second beta-propeller

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0001434)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G04140 AT1G04140 AT5G43930 AT5G43930 AT5G43930 AT5G43930
fragaria_vesca	FvH4_2g20210 FvH4_2g20210 FvH4_2g20210 FvH4_2g20210 FvH4_2g20210 FvH4_2g20210 FvH4_2g20210 FvH4_2g20210 FvH4_2g20210 FvH4_4g05210 FvH4_4g05210 FvH4_4g05210
malus_domestica	MD05G1185600.v1.1 MD10G1173800.v1.1 MD13G1226100.v1.1 MD16G1231400.v1.1
prunus_persica	Prupe.1G053600_v2.0.a1 Prupe.1G053600_v2.0.a1 Prupe.1G053600_v2.0.a1 Prupe.1G053600_v2.0.a1 Prupe.8G212400_v2.0.a1
pyrus_communis	pycom13g19940 pycom16g19360
rosa_chinensis	RchiOBHm_Chr4g0396141 RchiOBHm_Chr6g0285981
rosa_laevigata	RLG00000009566 RLG00000012592
rosa_multiflora	Rmu_co8311601.1_g000001
rosa_roxburghii	Rroxscaffold_5G00340990 Rroxscaffold_7G00182550
rosa_rugosa	Rorug03G0351500 Rorug03G0351600 Rorug03G0351700 Rorug03G0351800 Rorug03G0351900 Rorug06G0177900 Rorug06G0178000
rosa_samantha	Rh4BG065000 Rh4CG071800 Rh4DG061700 Rh6AG290000 Rh6BG292000 Rh6CG293000 Rh6DG285100
rosa_wichuraiana	Rw4G005370 Rw6G024990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1497
Acc36I	ACCTGC	1 cut(s)	1159
AccB1I	GGYRCC	1 cut(s)	1700
AccI	GTMKAC	2 cut(s)	1454, 2073
AccII	CGCG	3 cut(s)	52, 54, 1805
AccIII	TCCGGA	1 cut(s)	1869
AciI	CCGC	7 cut(s)	50, 128, 287, 716, 1012, 1297, 1719
AclWI	GGATC	8 cut(s)	407, 501, 547, 989, 1471, 1766, 2194, 2207
AcoI	YGGCCR	2 cut(s)	1610, 1993
AcsI	RAATTY	1 cut(s)	1605
AcuI	CTGAAG	8 cut(s)	191, 310, 698, 990, 1020, 1158, 1209, 2136
AcvI	CACGTG	1 cut(s)	1337
AdeI	CACNNNGTG	1 cut(s)	1340
AfaI	GTAC	4 cut(s)	626, 675, 1432, 1683
AfiI	CCNNNNNNNGG	2 cut(s)	2156, 2187
AflII	CTTAAG	1 cut(s)	2015
AgsI	TTSAA	6 cut(s)	206, 354, 972, 1029, 1070, 1624
AjnI	CCWGG	3 cut(s)	1397, 1774, 1788
AjuI	GAANNNNNNNTTGG	2 cut(s)	1881, 1913
AleI	CACNNNNGTG	1 cut(s)	44
Alw21I	GWGCWC	2 cut(s)	727, 1869
Alw26I	GTCTC	5 cut(s)	241, 357, 647, 1094, 2033
AlwI	GGATC	8 cut(s)	407, 501, 547, 989, 1471, 1766, 2194, 2207
AlwNI	CAGNNNCTG	1 cut(s)	1574
Ama87I	CYCGRG	1 cut(s)	1917
Aor13HI	TCCGGA	1 cut(s)	1869
AoxI	GGCC	5 cut(s)	193, 908, 1610, 1993, 2164
ApoI	RAATTY	1 cut(s)	1605
ArsI	GACNNNNNNTTYG	4 cut(s)	128, 160, 673, 705
AspLEI	GCGC	3 cut(s)	54, 810, 1498
AspS9I	GGNCC	2 cut(s)	194, 2272
AsuC2I	CCSGG	5 cut(s)	698, 798, 1423, 1918, 1919
AsuHPI	GGTGA	7 cut(s)	416, 602, 654, 704, 1232, 1319, 1352
AsuNHI	GCTAGC	1 cut(s)	1280
AvaI	CYCGRG	1 cut(s)	1917
AvaII	GGWCC	1 cut(s)	2272
BaeGI	GKGCMC	1 cut(s)	1705
BalI	TGGCCA	1 cut(s)	1612
BamHI	GGATCC	1 cut(s)	2199
BanI	GGYRCC	1 cut(s)	1700
BanII	GRGCYC	3 cut(s)	27, 727, 1869
BarI	GAAGNNNNNNTAC	2 cut(s)	861, 893
BauI	CACGAG	1 cut(s)	543
BbrPI	CACGTG	1 cut(s)	1337
BbsI	GAAGAC	2 cut(s)	264, 686
Bbv12I	GWGCWC	2 cut(s)	727, 1869
BbvCI	CCTCAGC	2 cut(s)	60, 744
BccI	CCATC	7 cut(s)	329, 833, 866, 1336, 1339, 1690, 2027
BceAI	ACGGC	2 cut(s)	540, 1980
BciT130I	CCWGG	3 cut(s)	1399, 1776, 1790
BciVI	GTATCC	1 cut(s)	474
BcnI	CCSGG	5 cut(s)	698, 798, 1423, 1918, 1919
BcoDI	GTCTC	5 cut(s)	241, 357, 647, 1094, 2033
BfmI	CTRYAG	4 cut(s)	1486, 1563, 1575, 2074
BfrI	CTTAAG	1 cut(s)	2015
BfuAI	ACCTGC	1 cut(s)	1159
BfuI	GTATCC	1 cut(s)	474
BglII	AGATCT	1 cut(s)	1412
BmcAI	AGTACT	1 cut(s)	675
Bme1390I	CCNGG	8 cut(s)	698, 798, 1399, 1423, 1776, 1790, 1918, 1919
Bme18I	GGWCC	1 cut(s)	2272
BmeT110I	CYCGRG	1 cut(s)	1917
BmgT120I	GGNCC	2 cut(s)	194, 2272
BmiI	GGNNCC	5 cut(s)	165, 1702, 2179, 2201, 2274
BmrFI	CCNGG	8 cut(s)	698, 798, 1399, 1423, 1776, 1790, 1918, 1919
BmrI	ACTGGG	1 cut(s)	405
BmsI	GCATC	9 cut(s)	208, 505, 575, 946, 1300, 1435, 1636, 1823, 1922
BmtI	GCTAGC	1 cut(s)	1284
BmuI	ACTGGG	1 cut(s)	405
BpiI	GAAGAC	2 cut(s)	264, 686
BplI	GAGNNNNNCTC	2 cut(s)	644, 676
BpmI	CTGGAG	3 cut(s)	245, 1071, 2087
Bpu10I	CCTNAGC	2 cut(s)	60, 744
BpuEI	CTTGAG	4 cut(s)	436, 1141, 1904, 2288
BpuMI	CCSGG	5 cut(s)	698, 798, 1423, 1918, 1919
BsaAI	YACGTR	1 cut(s)	1337
BsaI	GGTCTC	1 cut(s)	357
BsaJI	CCNNGG	7 cut(s)	302, 437, 586, 1368, 1917, 2181, 2286
BsaWI	WCCGGW	1 cut(s)	1869
BsaXI	ACNNNNNCTCC	6 cut(s)	239, 269, 678, 708, 746, 776
Bsc4I	CCNNNNNNNGG	2 cut(s)	2156, 2187
Bse1I	ACTGG	5 cut(s)	400, 861, 1383, 1411, 1520
Bse3DI	GCAATG	4 cut(s)	97, 561, 1161, 2221
BseAI	TCCGGA	1 cut(s)	1869
BseBI	CCWGG	3 cut(s)	1399, 1776, 1790
BseDI	CCNNGG	7 cut(s)	302, 437, 586, 1368, 1917, 2181, 2286
BseGI	GGATG	8 cut(s)	454, 520, 566, 656, 1834, 1913, 2128, 2146
BseLI	CCNNNNNNNGG	2 cut(s)	2156, 2187
BseMI	GCAATG	4 cut(s)	97, 561, 1161, 2221
BseMII	CTCAG	4 cut(s)	74, 735, 780, 1615
BseNI	ACTGG	5 cut(s)	400, 861, 1383, 1411, 1520
BseRI	GAGGAG	3 cut(s)	288, 754, 1210
BseSI	GKGCMC	1 cut(s)	1705
BsgI	GTGCAG	2 cut(s)	1317, 2136
Bsh1236I	CGCG	3 cut(s)	52, 54, 1805
BshFI	GGCC	5 cut(s)	195, 910, 1612, 1995, 2166
BshNI	GGYRCC	1 cut(s)	1700
BsiHKAI	GWGCWC	2 cut(s)	727, 1869
BsiHKCI	CYCGRG	1 cut(s)	1917
BsiSI	CCGG	5 cut(s)	697, 797, 1423, 1870, 1918
BslFI	GGGAC	4 cut(s)	543, 1045, 1807, 2258
BslI	CCNNNNNNNGG	2 cut(s)	2156, 2187
BsmAI	GTCTC	5 cut(s)	241, 357, 647, 1094, 2033
BsmFI	GGGAC	4 cut(s)	543, 1045, 1807, 2258
BsmI	GAATGC	1 cut(s)	2297
BsnI	GGCC	5 cut(s)	195, 910, 1612, 1995, 2166
Bso31I	GGTCTC	1 cut(s)	357
BsoBI	CYCGRG	1 cut(s)	1917
Bsp1286I	GDGCHC	4 cut(s)	27, 727, 1705, 1869
Bsp13I	TCCGGA	1 cut(s)	1869
Bsp1407I	TGTACA	1 cut(s)	624
Bsp143I	GATC	9 cut(s)	399, 493, 539, 853, 994, 1412, 1463, 1771, 2199
Bsp19I	CCATGG	1 cut(s)	437
BspACI	CCGC	7 cut(s)	50, 128, 287, 716, 1012, 1297, 1719
BspANI	GGCC	5 cut(s)	195, 910, 1612, 1995, 2166
BspCNI	CTCAG	4 cut(s)	73, 736, 779, 1614
BspEI	TCCGGA	1 cut(s)	1869
BspFNI	CGCG	3 cut(s)	52, 54, 1805
BspHI	TCATGA	1 cut(s)	496
BspLI	GGNNCC	5 cut(s)	165, 1702, 2179, 2201, 2274
BspMAI	CTGCAG	2 cut(s)	1567, 1579
BspMI	ACCTGC	1 cut(s)	1159
BspOI	GCTAGC	1 cut(s)	1284
BspPI	GGATC	8 cut(s)	407, 501, 547, 989, 1471, 1766, 2194, 2207
BspQI	GCTCTTC	1 cut(s)	1087
BspT107I	GGYRCC	1 cut(s)	1700
BspTI	CTTAAG	1 cut(s)	2015
BspTNI	GGTCTC	1 cut(s)	357
BsrDI	GCAATG	4 cut(s)	97, 561, 1161, 2221
BsrGI	TGTACA	1 cut(s)	624
BsrI	ACTGG	5 cut(s)	400, 861, 1383, 1411, 1520
BssECI	CCNNGG	7 cut(s)	302, 437, 586, 1368, 1917, 2181, 2286
BssMI	GATC	9 cut(s)	399, 493, 539, 853, 994, 1412, 1463, 1771, 2199
BssSI	CACGAG	1 cut(s)	543
BssT1I	CCWWGG	4 cut(s)	437, 586, 2181, 2286
Bst2BI	CACGAG	1 cut(s)	543
Bst2UI	CCWGG	3 cut(s)	1399, 1776, 1790
Bst4CI	ACNGT	9 cut(s)	83, 306, 373, 428, 826, 1163, 1594, 2006, 2047
Bst6I	CTCTTC	2 cut(s)	1087, 1092
BstAFI	CTTAAG	1 cut(s)	2015
BstAPI	GCANNNNNTGC	1 cut(s)	1574
BstAUI	TGTACA	1 cut(s)	624
BstBAI	YACGTR	1 cut(s)	1337
BstC8I	GCNNGC	4 cut(s)	77, 600, 1282, 1500
BstDEI	CTNAG	5 cut(s)	60, 98, 744, 766, 1601
BstDSI	CCRYGG	2 cut(s)	302, 437
BstEII	GGTNACC	1 cut(s)	422
BstF5I	GGATG	8 cut(s)	454, 520, 566, 656, 1834, 1913, 2128, 2146
BstFNI	CGCG	3 cut(s)	52, 54, 1805
BstHHI	GCGC	3 cut(s)	54, 810, 1498
BstKTI	GATC	9 cut(s)	402, 496, 542, 856, 997, 1415, 1466, 1774, 2202
BstMAI	GTCTC	5 cut(s)	241, 357, 647, 1094, 2033
BstMBI	GATC	9 cut(s)	399, 493, 539, 853, 994, 1412, 1463, 1771, 2199
BstNI	CCWGG	3 cut(s)	1399, 1776, 1790
BstNSI	RCATGY	4 cut(s)	1502, 1679, 1749, 2266
BstPI	GGTNACC	1 cut(s)	422
BstSCI	CCNGG	8 cut(s)	696, 796, 1397, 1421, 1774, 1788, 1916, 1917
BstSFI	CTRYAG	4 cut(s)	1486, 1563, 1575, 2074
BstSLI	GKGCMC	1 cut(s)	1705
BstUI	CGCG	3 cut(s)	52, 54, 1805
BstV2I	GAAGAC	2 cut(s)	264, 686
BstX2I	RGATCY	5 cut(s)	399, 539, 1412, 1463, 2199
BstYI	RGATCY	5 cut(s)	399, 539, 1412, 1463, 2199
BsuI	GTATCC	1 cut(s)	474
BsuRI	GGCC	5 cut(s)	195, 910, 1612, 1995, 2166
BtgI	CCRYGG	2 cut(s)	302, 437
BtsCI	GGATG	8 cut(s)	454, 520, 566, 656, 1834, 1913, 2128, 2146
BtsI	GCAGTG	1 cut(s)	1936
BtsIMutI	CAGTG	6 cut(s)	88, 1014, 1390, 1590, 1936, 2052
BveI	ACCTGC	1 cut(s)	1159
Cac8I	GCNNGC	4 cut(s)	77, 600, 1282, 1500
CaiI	CAGNNNCTG	1 cut(s)	1574
CciI	TCATGA	1 cut(s)	496
CfoI	GCGC	3 cut(s)	54, 810, 1498
Cfr13I	GGNCC	2 cut(s)	194, 2272
Cfr9I	CCCGGG	1 cut(s)	1917
CseI	GACGC	3 cut(s)	545, 597, 1811
Csp6I	GTAC	4 cut(s)	625, 674, 1431, 1682
CspCI	CAANNNNNGTGG	2 cut(s)	1602, 1637
CviQI	GTAC	4 cut(s)	625, 674, 1431, 1682
DdeI	CTNAG	5 cut(s)	60, 98, 744, 766, 1601
DpnI	GATC	9 cut(s)	401, 495, 541, 855, 996, 1414, 1465, 1773, 2201
DpnII	GATC	9 cut(s)	399, 493, 539, 853, 994, 1412, 1463, 1771, 2199
DraIII	CACNNNGTG	1 cut(s)	1340
EaeI	YGGCCR	2 cut(s)	1610, 1993
Eam1104I	CTCTTC	2 cut(s)	1087, 1092
EarI	CTCTTC	2 cut(s)	1087, 1092
Ecl136II	GAGCTC	2 cut(s)	725, 1867
Eco130I	CCWWGG	4 cut(s)	437, 586, 2181, 2286
Eco24I	GRGCYC	3 cut(s)	27, 727, 1869
Eco31I	GGTCTC	1 cut(s)	357
Eco47I	GGWCC	1 cut(s)	2272
Eco53kI	GAGCTC	2 cut(s)	725, 1867
Eco57I	CTGAAG	8 cut(s)	191, 310, 698, 990, 1020, 1158, 1209, 2136
Eco72I	CACGTG	1 cut(s)	1337
Eco88I	CYCGRG	1 cut(s)	1917
Eco91I	GGTNACC	1 cut(s)	422
EcoICRI	GAGCTC	2 cut(s)	725, 1867
EcoO109I	RGGNCCY	1 cut(s)	2272
EcoO65I	GGTNACC	1 cut(s)	422
EcoRII	CCWGG	3 cut(s)	1397, 1774, 1788
EcoT14I	CCWWGG	4 cut(s)	437, 586, 2181, 2286
EcoT38I	GRGCYC	3 cut(s)	27, 727, 1869
ErhI	CCWWGG	4 cut(s)	437, 586, 2181, 2286
FaqI	GGGAC	4 cut(s)	543, 1045, 1807, 2258
FauI	CCCGC	1 cut(s)	723
FauNDI	CATATG	4 cut(s)	629, 1446, 1761, 2281
FblI	GTMKAC	2 cut(s)	1454, 2073
FokI	GGATG	8 cut(s)	441, 527, 553, 643, 1821, 1900, 2133, 2135
FriOI	GRGCYC	3 cut(s)	27, 727, 1869
FspI	TGCGCA	1 cut(s)	1497
GlaI	GCGC	3 cut(s)	53, 809, 1497
GsuI	CTGGAG	3 cut(s)	245, 1071, 2087
HaeIII	GGCC	5 cut(s)	195, 910, 1612, 1995, 2166
HapII	CCGG	5 cut(s)	697, 797, 1423, 1870, 1918
HgaI	GACGC	3 cut(s)	545, 597, 1811
HhaI	GCGC	3 cut(s)	54, 810, 1498
Hin6I	GCGC	3 cut(s)	52, 808, 1496
HinP1I	GCGC	3 cut(s)	52, 808, 1496
HincII	GTYRAC	4 cut(s)	309, 1455, 1795, 2131
HindII	GTYRAC	4 cut(s)	309, 1455, 1795, 2131
HinfI	GANTC	8 cut(s)	185, 254, 761, 770, 1223, 1482, 1533, 2230
HpaII	CCGG	5 cut(s)	697, 797, 1423, 1870, 1918
HphI	GGTGA	7 cut(s)	416, 602, 654, 704, 1232, 1319, 1352
Hpy166II	GTNNAC	6 cut(s)	309, 1455, 1795, 2009, 2074, 2131
Hpy8I	GTNNAC	6 cut(s)	309, 1455, 1795, 2009, 2074, 2131
Hpy99I	CGWCG	3 cut(s)	23, 690, 2001
HpyAV	CCTTC	9 cut(s)	45, 334, 921, 1008, 1044, 1184, 1716, 2160, 2180
HpyCH4III	ACNGT	9 cut(s)	83, 306, 373, 428, 826, 1163, 1594, 2006, 2047
HpyCH4IV	ACGT	5 cut(s)	688, 1336, 1820, 1967, 2133
HpyF3I	CTNAG	5 cut(s)	60, 98, 744, 766, 1601
HpySE526I	ACGT	5 cut(s)	688, 1336, 1820, 1967, 2133
HspAI	GCGC	3 cut(s)	52, 808, 1496
Kpn2I	TCCGGA	1 cut(s)	1869
Kzo9I	GATC	9 cut(s)	399, 493, 539, 853, 994, 1412, 1463, 1771, 2199
LguI	GCTCTTC	1 cut(s)	1087
LmnI	GCTCC	5 cut(s)	699, 722, 730, 1197, 1872
LweI	GCATC	9 cut(s)	208, 505, 575, 946, 1300, 1435, 1636, 1823, 1922
MaeII	ACGT	5 cut(s)	688, 1336, 1820, 1967, 2133
MaeIII	GTNAC	4 cut(s)	255, 422, 1220, 2000
MalI	GATC	9 cut(s)	401, 495, 541, 855, 996, 1414, 1465, 1773, 2201
MboI	GATC	9 cut(s)	399, 493, 539, 853, 994, 1412, 1463, 1771, 2199
MboII	GAAGA	8 cut(s)	6, 264, 691, 881, 1074, 1109, 1422, 2099
MfeI	CAATTG	2 cut(s)	1065, 1458
MflI	RGATCY	5 cut(s)	399, 539, 1412, 1463, 2199
MhlI	GDGCHC	4 cut(s)	27, 727, 1705, 1869
MlsI	TGGCCA	1 cut(s)	1612
MluCI	AATT	7 cut(s)	28, 178, 1065, 1263, 1458, 1605, 1839
MluNI	TGGCCA	1 cut(s)	1612
MlyI	GAGTC	3 cut(s)	263, 755, 1217
MmeI	TCCRAC	2 cut(s)	42, 2137
Mox20I	TGGCCA	1 cut(s)	1612
MroI	TCCGGA	1 cut(s)	1869
MscI	TGGCCA	1 cut(s)	1612
MseI	TTAA	7 cut(s)	407, 737, 1262, 1664, 1943, 2016, 2195
MslI	CAYNNNNRTG	3 cut(s)	44, 639, 717
Msp20I	TGGCCA	1 cut(s)	1612
MspA1I	CMGCKG	1 cut(s)	1574
MspCI	CTTAAG	1 cut(s)	2015
MspI	CCGG	5 cut(s)	697, 797, 1423, 1870, 1918
MspR9I	CCNGG	8 cut(s)	698, 798, 1399, 1423, 1776, 1790, 1918, 1919
MunI	CAATTG	2 cut(s)	1065, 1458
Mva1269I	GAATGC	1 cut(s)	2297
MvaI	CCWGG	3 cut(s)	1399, 1776, 1790
MvnI	CGCG	3 cut(s)	52, 54, 1805
NciI	CCSGG	5 cut(s)	698, 798, 1423, 1918, 1919
NcoI	CCATGG	1 cut(s)	437
NdeI	CATATG	4 cut(s)	629, 1446, 1761, 2281
NdeII	GATC	9 cut(s)	399, 493, 539, 853, 994, 1412, 1463, 1771, 2199
NheI	GCTAGC	1 cut(s)	1280
NlaIV	GGNNCC	5 cut(s)	165, 1702, 2179, 2201, 2274
NmuCI	GTSAC	4 cut(s)	255, 422, 1220, 2000
NsbI	TGCGCA	1 cut(s)	1497
NspI	RCATGY	4 cut(s)	1502, 1679, 1749, 2266
OliI	CACNNNNGTG	1 cut(s)	44
PaeI	GCATGC	1 cut(s)	1502
PagI	TCATGA	1 cut(s)	496
PciSI	GCTCTTC	1 cut(s)	1087
PcsI	WCGNNNNNNNCGW	1 cut(s)	1435
PctI	GAATGC	1 cut(s)	2297
PfeI	GAWTC	5 cut(s)	185, 770, 1482, 1533, 2230
PfoI	TCCNGGA	1 cut(s)	1774
PleI	GAGTC	3 cut(s)	262, 755, 1217
PmaCI	CACGTG	1 cut(s)	1337
PmlI	CACGTG	1 cut(s)	1337
PpsI	GAGTC	3 cut(s)	262, 755, 1217
Ppu21I	YACGTR	1 cut(s)	1337
PpuMI	RGGWCCY	1 cut(s)	2272
Psp124BI	GAGCTC	2 cut(s)	727, 1869
Psp5II	RGGWCCY	1 cut(s)	2272
Psp6I	CCWGG	3 cut(s)	1397, 1774, 1788
PspCI	CACGTG	1 cut(s)	1337
PspEI	GGTNACC	1 cut(s)	422
PspGI	CCWGG	3 cut(s)	1397, 1774, 1788
PspN4I	GGNNCC	5 cut(s)	165, 1702, 2179, 2201, 2274
PspPI	GGNCC	2 cut(s)	194, 2272
PspPPI	RGGWCCY	1 cut(s)	2272
PstI	CTGCAG	2 cut(s)	1567, 1579
PstNI	CAGNNNCTG	1 cut(s)	1574
PsuI	RGATCY	5 cut(s)	399, 539, 1412, 1463, 2199
PvuII	CAGCTG	1 cut(s)	1574
RsaI	GTAC	4 cut(s)	626, 675, 1432, 1683
RsaNI	GTAC	4 cut(s)	625, 674, 1431, 1682
RseI	CAYNNNNRTG	3 cut(s)	44, 639, 717
SacI	GAGCTC	2 cut(s)	727, 1869
SalI	GTCGAC	1 cut(s)	1453
SapI	GCTCTTC	1 cut(s)	1087
SaqAI	TTAA	7 cut(s)	407, 737, 1262, 1664, 1943, 2016, 2195
Sau3AI	GATC	9 cut(s)	399, 493, 539, 853, 994, 1412, 1463, 1771, 2199
Sau96I	GGNCC	2 cut(s)	194, 2272
ScaI	AGTACT	1 cut(s)	675
SchI	GAGTC	3 cut(s)	263, 755, 1217
ScrFI	CCNGG	8 cut(s)	698, 798, 1399, 1423, 1776, 1790, 1918, 1919
SduI	GDGCHC	4 cut(s)	27, 727, 1705, 1869
SfaNI	GCATC	9 cut(s)	208, 505, 575, 946, 1300, 1435, 1636, 1823, 1922
SfcI	CTRYAG	4 cut(s)	1486, 1563, 1575, 2074
SinI	GGWCC	1 cut(s)	2272
SmaI	CCCGGG	1 cut(s)	1919
SmiMI	CAYNNNNRTG	3 cut(s)	44, 639, 717
SmlI	CTYRAG	5 cut(s)	415, 1120, 1883, 2015, 2267
SmoI	CTYRAG	5 cut(s)	415, 1120, 1883, 2015, 2267
SphI	GCATGC	1 cut(s)	1502
Sse9I	AATT	7 cut(s)	28, 178, 1065, 1263, 1458, 1605, 1839
SsiI	CCGC	7 cut(s)	50, 128, 287, 716, 1012, 1297, 1719
SspI	AATATT	1 cut(s)	281
SstI	GAGCTC	2 cut(s)	727, 1869
StyD4I	CCNGG	8 cut(s)	696, 796, 1397, 1421, 1774, 1788, 1916, 1917
StyI	CCWWGG	4 cut(s)	437, 586, 2181, 2286
TaaI	ACNGT	9 cut(s)	83, 306, 373, 428, 826, 1163, 1594, 2006, 2047
TaiI	ACGT	5 cut(s)	691, 1339, 1823, 1970, 2136
TaqI	TCGA	3 cut(s)	776, 1454, 1831
TasI	AATT	7 cut(s)	28, 178, 1065, 1263, 1458, 1605, 1839
TatI	WGTACW	3 cut(s)	624, 673, 1681
TauI	GCSGC	2 cut(s)	52, 1300
TfiI	GAWTC	5 cut(s)	185, 770, 1482, 1533, 2230
Tru1I	TTAA	7 cut(s)	407, 737, 1262, 1664, 1943, 2016, 2195
Tru9I	TTAA	7 cut(s)	407, 737, 1262, 1664, 1943, 2016, 2195
TscAI	CASTG	6 cut(s)	88, 1014, 1390, 1597, 1943, 2052
TseFI	GTSAC	4 cut(s)	255, 422, 1220, 2000
Tsp45I	GTSAC	4 cut(s)	255, 422, 1220, 2000
TspDTI	ATGAA	8 cut(s)	443, 513, 903, 1062, 1485, 2135, 2243, 2271
TspGWI	ACGGA	4 cut(s)	60, 127, 375, 1303
TspMI	CCCGGG	1 cut(s)	1917
TspRI	CASTG	6 cut(s)	88, 1014, 1390, 1597, 1943, 2052
Vha464I	CTTAAG	1 cut(s)	2015
VpaK11BI	GGWCC	1 cut(s)	2272
XapI	RAATTY	1 cut(s)	1605
XceI	RCATGY	4 cut(s)	1502, 1679, 1749, 2266
XcmI	CCANNNNNNNNNTGG	1 cut(s)	903
XmaI	CCCGGG	1 cut(s)	1917
XmiI	GTMKAC	2 cut(s)	1454, 2073
ZrmI	AGTACT	1 cut(s)	675

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.