MD10G1173800.v1.1

WD domain, G-beta repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0001434
Physical Location & Seq
Forward (+)
26641578 .. 26648653
7076 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1173800.v1.1.491

Sequence Viewer

Length: 2487 bp
ATGAAGTCCGATCTTACGATGGCAGGACCTTCGCGGCCGTTGGATCAGACCCCGATTTCTCCCCCTCCGTCCTCCGAGCATCTTCCCTGCAGAAATGTGGTTGGCTTGCTAGCACGAAGGGAGATTTCACCTAGAGCAAAACATGTGCCCAGAAGGGGGTGGGGAGAAACTTCTAAAAGGAAAGCTAGTTCCTCCTATGGGCCTAAGGGTGAAGCAACAAGAGATGCAAAACGTGGCCTTCTCTCATGGGTTGAAGCAGAGTCACTGCGGCACTTGTCAGCCAAGTATTGTGATTTAGTGCCTCCTCCAAGGTCAACCATTGCAGCAGCATTCAGCCCCGATGGAAGAAAACTTGCTTCTACACATGGTGACCACACTGTAAAGATAATTGATTGCCTAACTGGCAGATGCGTAAAGGTTTTGAGCGGCCATAGAAGGACACCTTGGGTGGTTAGGTTTCATCCTCTGAATCCAGAAATAATTGCTAGTGGAAGTTTGGATCATGAAGTTCGCTTATGGGATTTAAACACATCAGATTGTATCGTCTCTCGTGATTTCTATCGACCTATTGCATCTATTGCTTTTCATGCCAAAGGGGAACTCCTAGCTGTTGCATCAGGACACAAGTTGTTTATATGGAAATACAATGGGGAACCTTCTTCTCCAGACATGGTATTGAAAACATGGCGTTCCCTTCGAGCAGTGCATTTTCACCCCCAAGCTGCTCCATTCCTCTTAACTGCTGAGGTCAATGATCTTGACTCTTCAGATTCCTCAATGTCTCAAGCAACATCTCCCGGCTACGTGCATTATCCATCTCCTGCTGTTTTTGTGGCAAACGCTGATTCTAGTGAACGTCTTAGTTTGGCTACTGAACTACCACTTATGTCCTTGCCTTTTGTCTTCGTGCTGCCATGTGTAGATGATCCAAGAATAGTAATGCAGCCTGAAAACGGGCCTGCTGGATCAAATAGCATGCAAGTCACCTCTGCTTCAATGCAGTTTCAAGCAGATGCAAATGCAGCAGAGCAGGATGCTACCACAATTTCTCCAATGGAGACATTTCCATCAGATCCAACTGTTTCCAATCTCAACACTGAAGGCAATGCAGATAATTCTTTCCTTAATGGAACGAGAAGTGGTATTTGTAACCGCACAGGAGATGCAATGGAGACTGATGAGATGCCGGCTGTAGGGGGAAGCCAGCATGGAAGCTTGACTAATTTTGATTCTGCGAATATTAGAGTTGAAAATACTTCAAATCATCCTGGTTTTACTGAATCTGGGCAACCTTATAGGACGTTTCCATATAGAGATCCTGCATTCTGGGAGCTACCTTTTCTCCAAGGGTGGTTAACAGGTCAAAGCCAAGCTAGTTTTCCCTCAATGCTTCCTCTAAATCGTGGTGGGCTTGACAATTTAGCTCAGTCTATGGGTTCTTCTAGTTTGGTGTCGCATCTGTCTGCGCATAATGTGGCGGATGTGGTAGCTTCTTTGGCATTGCCAGGAAGCAGCAGTGTATCTGGGCTTTCTGGAAGATCTGATTTGCAGCATTATCCACGGTTCCACTTCTCTGGACCAGAATCAGGGGATAGTACTGCCTTAAATACCCAACCCGATGAGAATGATGCTCAGCCCATTATTAGTAGAATCCAGTCTGATATTGATACATTAATGGCTGCAGCAGCAGCTGCAGAATTACCATGCACTGTAAAACTAAGAGTATGGTCGCATGATATAAAAAATCCTTCTGCTCTGCTGAATGATGAAAGATGTCGTTTAACCGTTCCCCATGCTGTTCTTTGCAGTGAAATGGGTGCTCATTTCTCCCCTTGTGGGAGATTTTTAGCTGCCTGTGTTGCGTGCATGCTTCCTCATACGGAAGCTGACCCTGGACTACAAAGTCTAGTTCCTCAGGATTCTGGAATTGCAACATCCCCGACTCGCCACCCAATCTCAGCGCACCAAGTTATGTATGAGCTTCGCATATATTCACTTGAGGAAGCCACATTTGGTTCGATACTTGTGTCTCGGGCTATTAGAGCAGCACATTGTTTGACTTCAATCCAATTCTCCCCCACGTCTGAGCACATATTATTGGCTTATGGTCGACGCCATGGTTCTCTTCTTAAAAGTATTGTCATTGATGGGGATACAACCTTACCTATTTACACTGTTCTTGAGGTTTACAGAGTTTCAGACATGGAACTTGTGAGAGTACTTCCCAGTGCAGAGGATGAGGTTAATGTTGCTTGCTTTCATCCCTTTGCTGGTGGAGGTCTGGTTTATGGGACCAAGGAAGGGAAGCTTAGGGTACTCCAGTTTGATGGTACCCATGATGAAAATTTCGTTGGACCAAAATACTTTACAGAGGAAAATACAGCTGTTGCAATATTGAGGGAAATGGTTCAGAGATTGCTTGCTGAGCATATATATTATAAACTCCAAAAGCCTAGCCTTTTTCCTTCAGGATCCATGGCAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000045 GO:0000422 GO:0000423 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0005856 GO:0005929 GO:0005930 GO:0006914 GO:0006950 GO:0006996 GO:0007005 GO:0007033 GO:0007275 GO:0007399 GO:0008150 GO:0008152 GO:0008285 GO:0009056 GO:0009790 GO:0009792 GO:0009888 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010506 GO:0010508 GO:0010562 GO:0010656 GO:0010660 GO:0010662 GO:0010664 GO:0010665 GO:0010667 GO:0010941 GO:0016043 GO:0016236 GO:0019216 GO:0019220 GO:0019222 GO:0019899 GO:0021915 GO:0022411 GO:0022607 GO:0030139 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0031410 GO:0031625 GO:0031982 GO:0032501 GO:0032502 GO:0032838 GO:0033554 GO:0033674 GO:0035295 GO:0042127 GO:0042325 GO:0042327 GO:0042981 GO:0042995 GO:0043009 GO:0043066 GO:0043067 GO:0043069 GO:0043085 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043523 GO:0043524 GO:0043549 GO:0043550 GO:0043551 GO:0043552 GO:0044085 GO:0044093 GO:0044237 GO:0044248 GO:0044389 GO:0044422 GO:0044424 GO:0044430 GO:0044441 GO:0044444 GO:0044446 GO:0044463 GO:0044464 GO:0045335 GO:0045834 GO:0045937 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051020 GO:0051174 GO:0051338 GO:0051347 GO:0051716 GO:0060429 GO:0060548 GO:0061726 GO:0061912 GO:0061919 GO:0065007 GO:0065009 GO:0070925 GO:0071840 GO:0080090 GO:0090218 GO:0097014 GO:0097708 GO:0098780 GO:0099568 GO:0120025 GO:0120038 GO:1901214 GO:1901215 GO:1903008 GO:1903725 GO:1903727 GO:1905037
KEGG Pathways
Metabolic & Signaling
ko04137 ko04140 map04137 map04140
Pfam Domains
Protein Families
WD40

Protein Analysis

829

Amino Acids

90.03

Weight (kDa)

6.03

Isoelectric Point (pI)

49.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Prp19 PF24814 107 - 241 3.4e-10 Prp19 WD40 domain
WD40_Gbeta PF25391 107 - 203 7.1e-06 G protein beta WD-40 repeat protein
Beta-prop_WDR3_1st PF25173 108 - 214 3.2e-13 WDR3 first beta-propeller domain
WDR55 PF24796 108 - 208 5.3e-09 WDR55
Beta-prop_THOC3 PF25174 109 - 249 9.9e-19 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 110 - 251 1e-18 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 110 - 210 1.3e-10 WDHD1 first WD40 domain
WD40_CDC20-Fz PF24807 110 - 216 2.3e-10 CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 114 - 214 8.3e-08 WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2 PF23414 149 - 208 2.8e-06 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2439
AasI GACNNNNNNGTC 1 cut(s) 1902
Acc16I TGCGCA 1 cut(s) 1467
Acc65I GGTACC 1 cut(s) 2330
AccB1I GGYRCC 1 cut(s) 2330
AccBSI CCGCTC 1 cut(s) 426
AccI GTMKAC 1 cut(s) 2110
AccII CGCG 1 cut(s) 34
AciI CCGC 5 cut(s) 34, 268, 426, 1153, 1478
AclWI GGATC 8 cut(s) 51, 507, 920, 973, 1067, 1310, 2466, 2479
AcoI YGGCCR 2 cut(s) 35, 427
AcsI RAATTY 1 cut(s) 2344
AcuI CTGAAG 3 cut(s) 750, 1119, 2451
AcyI GRCGYC 1 cut(s) 2113
AdeI CACNNNGTG 1 cut(s) 368
AfaI GTAC 4 cut(s) 1597, 2220, 2316, 2332
AfiI CCNNNNNNNGG 9 cut(s) 155, 156, 198, 953, 1193, 1586, 1836, 2270, 2301
AflIII ACRYGT 1 cut(s) 142
AgsI TTSAA 7 cut(s) 254, 679, 996, 1007, 1250, 1260, 2064
AjiI CACGTC 1 cut(s) 2082
AjnI CCWGG 3 cut(s) 1267, 1504, 1891
AjuI GAANNNNNNNTTGG 8 cut(s) 427, 459, 1362, 1394, 1995, 2027, 2334, 2366
AloI GAACNNNNNNTCC 2 cut(s) 172, 204
Alw21I GWGCWC 2 cut(s) 1822, 2091
Alw26I GTCTC 5 cut(s) 550, 786, 1052, 1166, 2034
AlwI GGATC 8 cut(s) 51, 507, 920, 973, 1067, 1310, 2466, 2479
AlwNI CAGNNNCTG 1 cut(s) 1691
Ama87I CYCGRG 1 cut(s) 2031
AoxI GGCC 5 cut(s) 35, 200, 235, 427, 956
ApoI RAATTY 1 cut(s) 2344
AseI ATTAAT 1 cut(s) 1673
Asp700I GAANNNNTTC 1 cut(s) 2406
Asp718I GGTACC 1 cut(s) 2330
AspLEI GCGC 2 cut(s) 1468, 1963
AspS9I GGNCC 6 cut(s) 26, 200, 956, 1577, 2292, 2354
AsuC2I CCSGG 1 cut(s) 798
AsuHPI GGTGA 5 cut(s) 120, 221, 380, 704, 976
AsuNHI GCTAGC 1 cut(s) 109
AvaI CYCGRG 1 cut(s) 2031
AvaII GGWCC 4 cut(s) 26, 1577, 2292, 2354
AxyI CCTNAGG 2 cut(s) 204, 1914
BaeGI GKGCMC 1 cut(s) 150
BaeI ACNNNNGTAYC 4 cut(s) 2012, 2045, 2306, 2339
BamHI GGATCC 1 cut(s) 2471
BanI GGYRCC 1 cut(s) 2330
BauI CACGAG 1 cut(s) 549
BbsI GAAGAC 1 cut(s) 895
Bbv12I GWGCWC 2 cut(s) 1822, 2091
BbvCI CCTCAGC 1 cut(s) 744
BccI CCATC 6 cut(s) 13, 335, 823, 1075, 2141, 2321
BceAI ACGGC 1 cut(s) 22
BcgI CGANNNNNNTGC 2 cut(s) 12, 46
BciT130I CCWGG 3 cut(s) 1269, 1506, 1893
BciVI GTATCC 1 cut(s) 2146
BcnI CCSGG 1 cut(s) 798
BcoDI GTCTC 5 cut(s) 550, 786, 1052, 1166, 2034
BfmI CTRYAG 4 cut(s) 88, 1191, 1680, 1692
BfuI GTATCC 1 cut(s) 2146
BglI GCCNNNNNGGC 1 cut(s) 402
BglII AGATCT 1 cut(s) 1538
BlpI GCTNAGC 2 cut(s) 1632, 2424
BmcAI AGTACT 2 cut(s) 1597, 2220
Bme1390I CCNGG 4 cut(s) 798, 1269, 1506, 1893
Bme18I GGWCC 4 cut(s) 26, 1577, 2292, 2354
BmeT110I CYCGRG 1 cut(s) 2031
BmgBI CACGTC 1 cut(s) 2082
BmgT120I GGNCC 6 cut(s) 26, 200, 956, 1577, 2292, 2354
BmiI GGNNCC 5 cut(s) 654, 1565, 2293, 2332, 2473
BmrFI CCNGG 4 cut(s) 798, 1269, 1506, 1893
BmrI ACTGGG 1 cut(s) 2220
BmtI GCTAGC 1 cut(s) 113
BmuI ACTGGG 1 cut(s) 2220
BpiI GAAGAC 1 cut(s) 895
BpmI CTGGAG 2 cut(s) 648, 2303
Bpu10I CCTNAGC 2 cut(s) 744, 2309
Bpu1102I GCTNAGC 2 cut(s) 1632, 2424
BpuEI CTTGAG 3 cut(s) 768, 2018, 2201
BpuMI CCSGG 1 cut(s) 798
BsaAI YACGTR 1 cut(s) 805
BsaBI GATNNNNATC 1 cut(s) 558
BsaHI GRCGYC 1 cut(s) 2113
BsaJI CCNNGG 8 cut(s) 308, 443, 1345, 1559, 1891, 2116, 2295, 2475
BsaXI ACNNNNNCTCC 6 cut(s) 1033, 1063, 1326, 1356, 2268, 2298
Bsc4I CCNNNNNNNGG 9 cut(s) 155, 156, 198, 953, 1193, 1586, 1836, 2270, 2301
Bse118I RCCGGY 1 cut(s) 1186
Bse1I ACTGG 4 cut(s) 406, 1654, 2226, 2320
Bse21I CCTNAGG 2 cut(s) 204, 1914
Bse3DI GCAATG 5 cut(s) 318, 1111, 1173, 1499, 2487
Bse8I GATNNNNATC 1 cut(s) 558
BseBI CCWGG 3 cut(s) 1269, 1506, 1893
BseDI CCNNGG 8 cut(s) 308, 443, 1345, 1559, 1891, 2116, 2295, 2475
BseGI GGATG 7 cut(s) 460, 1039, 1264, 1486, 1934, 2242, 2260
BseJI GATNNNNATC 1 cut(s) 558
BseLI CCNNNNNNNGG 9 cut(s) 155, 156, 198, 953, 1193, 1586, 1836, 2270, 2301
BseMI GCAATG 5 cut(s) 318, 1111, 1173, 1499, 2487
BseMII CTCAG 7 cut(s) 735, 1439, 1646, 1928, 1971, 2076, 2415
BseNI ACTGG 4 cut(s) 406, 1654, 2226, 2320
BseRI GAGGAG 1 cut(s) 294
BseSI GKGCMC 1 cut(s) 150
BseX3I CGGCCG 1 cut(s) 35
BsgI GTGCAG 1 cut(s) 2250
Bsh1236I CGCG 1 cut(s) 34
Bsh1285I CGRYCG 1 cut(s) 38
BshFI GGCC 5 cut(s) 37, 202, 237, 429, 958
BshNI GGYRCC 1 cut(s) 2330
BsiEI CGRYCG 1 cut(s) 38
BsiHKAI GWGCWC 2 cut(s) 1822, 2091
BsiHKCI CYCGRG 1 cut(s) 2031
BsiSI CCGG 2 cut(s) 798, 1187
BslFI GGGAC 1 cut(s) 2305
BslI CCNNNNNNNGG 9 cut(s) 155, 156, 198, 953, 1193, 1586, 1836, 2270, 2301
BsmAI GTCTC 5 cut(s) 550, 786, 1052, 1166, 2034
BsmBI CGTCTC 1 cut(s) 550
BsmFI GGGAC 1 cut(s) 2305
BsmI GAATGC 2 cut(s) 329, 1322
BsnI GGCC 5 cut(s) 37, 202, 237, 429, 958
BsoBI CYCGRG 1 cut(s) 2031
Bsp1286I GDGCHC 3 cut(s) 150, 1822, 2091
Bsp1720I GCTNAGC 2 cut(s) 1632, 2424
Bsp19I CCATGG 2 cut(s) 2116, 2475
BspACI CCGC 5 cut(s) 34, 268, 426, 1153, 1478
BspANI GGCC 5 cut(s) 37, 202, 237, 429, 958
BspCNI CTCAG 7 cut(s) 736, 1438, 1645, 1927, 1970, 2077, 2416
BspFNI CGCG 1 cut(s) 34
BspHI TCATGA 1 cut(s) 502
BspLI GGNNCC 5 cut(s) 654, 1565, 2293, 2332, 2473
BspMAI CTGCAG 3 cut(s) 92, 1684, 1696
BspOI GCTAGC 1 cut(s) 113
BspPI GGATC 8 cut(s) 51, 507, 920, 973, 1067, 1310, 2466, 2479
BspT107I GGYRCC 1 cut(s) 2330
BsrBI CCGCTC 1 cut(s) 426
BsrDI GCAATG 5 cut(s) 318, 1111, 1173, 1499, 2487
BsrFI RCCGGY 1 cut(s) 1186
BsrI ACTGG 4 cut(s) 406, 1654, 2226, 2320
BssAI RCCGGY 1 cut(s) 1186
BssECI CCNNGG 8 cut(s) 308, 443, 1345, 1559, 1891, 2116, 2295, 2475
BssNI GRCGYC 1 cut(s) 2113
BssSI CACGAG 1 cut(s) 549
BssT1I CCWWGG 6 cut(s) 308, 443, 1345, 2116, 2295, 2475
Bst2BI CACGAG 1 cut(s) 549
Bst2UI CCWGG 3 cut(s) 1269, 1506, 1893
Bst4CI ACNGT 6 cut(s) 379, 1081, 1563, 1711, 1786, 2176
Bst6I CTCTTC 2 cut(s) 769, 2130
BstACI GRCGYC 1 cut(s) 2113
BstAPI GCANNNNNTGC 2 cut(s) 578, 1691
BstBAI YACGTR 1 cut(s) 805
BstDSI CCRYGG 3 cut(s) 1559, 2116, 2475
BstEII GGTNACC 1 cut(s) 368
BstF5I GGATG 7 cut(s) 460, 1039, 1264, 1486, 1934, 2242, 2260
BstFNI CGCG 1 cut(s) 34
BstHHI GCGC 2 cut(s) 1468, 1963
BstMAI GTCTC 5 cut(s) 550, 786, 1052, 1166, 2034
BstMCI CGRYCG 1 cut(s) 38
BstNI CCWGG 3 cut(s) 1269, 1506, 1893
BstNSI RCATGY 3 cut(s) 146, 979, 1870
BstPI GGTNACC 1 cut(s) 368
BstSCI CCNGG 4 cut(s) 796, 1267, 1504, 1891
BstSFI CTRYAG 4 cut(s) 88, 1191, 1680, 1692
BstSLI GKGCMC 1 cut(s) 150
BstUI CGCG 1 cut(s) 34
BstV2I GAAGAC 1 cut(s) 895
BstX2I RGATCY 4 cut(s) 1072, 1315, 1538, 2471
BstXI CCANNNNNNTGG 2 cut(s) 1574, 2327
BstYI RGATCY 4 cut(s) 1072, 1315, 1538, 2471
BstZI CGGCCG 1 cut(s) 35
Bsu36I CCTNAGG 2 cut(s) 204, 1914
BsuI GTATCC 1 cut(s) 2146
BsuRI GGCC 5 cut(s) 37, 202, 237, 429, 958
BtgI CCRYGG 3 cut(s) 1559, 2116, 2475
BtrI CACGTC 1 cut(s) 2082
BtsCI GGATG 7 cut(s) 460, 1039, 1264, 1486, 1934, 2242, 2260
BtsI GCAGTG 4 cut(s) 263, 708, 1522, 1813
BtsIMutI CAGTG 9 cut(s) 263, 375, 708, 1095, 1522, 1707, 1813, 2172, 2233
CaiI CAGNNNCTG 1 cut(s) 1691
CciI TCATGA 1 cut(s) 502
CfoI GCGC 2 cut(s) 1468, 1963
Cfr10I RCCGGY 1 cut(s) 1186
Cfr13I GGNCC 6 cut(s) 26, 200, 956, 1577, 2292, 2354
CseI GACGC 1 cut(s) 2121
Csp6I GTAC 4 cut(s) 1596, 2219, 2315, 2331
CviQI GTAC 4 cut(s) 1596, 2219, 2315, 2331
DraI TTTAAA 1 cut(s) 525
DraIII CACNNNGTG 1 cut(s) 368
DrdI GACNNNNNNGTC 1 cut(s) 1902
DseDI GACNNNNNNGTC 1 cut(s) 1902
EaeI YGGCCR 2 cut(s) 35, 427
EagI CGGCCG 1 cut(s) 35
Eam1104I CTCTTC 2 cut(s) 769, 2130
EarI CTCTTC 2 cut(s) 769, 2130
EciI GGCGGA 1 cut(s) 1493
EclXI CGGCCG 1 cut(s) 35
Eco130I CCWWGG 6 cut(s) 308, 443, 1345, 2116, 2295, 2475
Eco47I GGWCC 4 cut(s) 26, 1577, 2292, 2354
Eco52I CGGCCG 1 cut(s) 35
Eco57I CTGAAG 3 cut(s) 750, 1119, 2451
Eco81I CCTNAGG 2 cut(s) 204, 1914
Eco88I CYCGRG 1 cut(s) 2031
Eco91I GGTNACC 1 cut(s) 368
EcoO109I RGGNCCY 1 cut(s) 26
EcoO65I GGTNACC 1 cut(s) 368
EcoRII CCWGG 3 cut(s) 1267, 1504, 1891
EcoT14I CCWWGG 6 cut(s) 308, 443, 1345, 2116, 2295, 2475
ErhI CCWWGG 6 cut(s) 308, 443, 1345, 2116, 2295, 2475
Esp3I CGTCTC 1 cut(s) 550
FalI AAGNNNNNCTT 4 cut(s) 427, 459, 2292, 2324
FaqI GGGAC 1 cut(s) 2305
FblI GTMKAC 1 cut(s) 2110
FokI GGATG 7 cut(s) 447, 1046, 1251, 1493, 1921, 2247, 2249
FspI TGCGCA 1 cut(s) 1467
GlaI GCGC 2 cut(s) 1467, 1962
GsuI CTGGAG 2 cut(s) 648, 2303
HaeIII GGCC 5 cut(s) 37, 202, 237, 429, 958
HapII CCGG 2 cut(s) 798, 1187
HgaI GACGC 1 cut(s) 2121
HhaI GCGC 2 cut(s) 1468, 1963
Hin1I GRCGYC 1 cut(s) 2113
Hin6I GCGC 2 cut(s) 1466, 1961
HinP1I GCGC 2 cut(s) 1466, 1961
HincII GTYRAC 3 cut(s) 315, 1356, 2111
HindII GTYRAC 3 cut(s) 315, 1356, 2111
HindIII AAGCTT 2 cut(s) 1213, 2306
HpaI GTTAAC 1 cut(s) 1356
HpaII CCGG 2 cut(s) 798, 1187
HphI GGTGA 5 cut(s) 120, 221, 380, 704, 976
Hpy166II GTNNAC 5 cut(s) 315, 854, 1356, 2111, 2188
Hpy8I GTNNAC 5 cut(s) 315, 854, 1356, 2111, 2188
Hpy99I CGWCG 1 cut(s) 2115
HpyCH4III ACNGT 6 cut(s) 379, 1081, 1563, 1711, 1786, 2176
HpyCH4IV ACGT 5 cut(s) 232, 804, 856, 1301, 2081
HpySE526I ACGT 5 cut(s) 232, 804, 856, 1301, 2081
Hsp92I GRCGYC 1 cut(s) 2113
HspAI GCGC 2 cut(s) 1466, 1961
KpnI GGTACC 1 cut(s) 2334
KroI GCCGGC 1 cut(s) 1186
KroNI GCCGGC 1 cut(s) 1188
KspAI GTTAAC 1 cut(s) 1356
LmnI GCTCC 2 cut(s) 730, 1330
MaeII ACGT 5 cut(s) 232, 804, 856, 1301, 2081
MaeIII GTNAC 4 cut(s) 261, 368, 982, 1148
MbiI CCGCTC 1 cut(s) 426
MboII GAAGA 8 cut(s) 74, 357, 651, 756, 895, 1431, 1548, 2117
MflI RGATCY 4 cut(s) 1072, 1315, 1538, 2471
MhlI GDGCHC 3 cut(s) 150, 1822, 2091
MlyI GAGTC 3 cut(s) 269, 755, 1936
MmeI TCCRAC 3 cut(s) 21, 1100, 2332
MroNI GCCGGC 1 cut(s) 1186
MroXI GAANNNNTTC 1 cut(s) 2406
MseI TTAA 9 cut(s) 524, 737, 1125, 1355, 1604, 1673, 1781, 2130, 2244
MslI CAYNNNNRTG 1 cut(s) 2480
MspA1I CMGCKG 2 cut(s) 1691, 2384
MspI CCGG 2 cut(s) 798, 1187
MspR9I CCNGG 4 cut(s) 798, 1269, 1506, 1893
Mva1269I GAATGC 2 cut(s) 329, 1322
MvaI CCWGG 3 cut(s) 1269, 1506, 1893
MvnI CGCG 1 cut(s) 34
NaeI GCCGGC 1 cut(s) 1188
NciI CCSGG 1 cut(s) 798
NcoI CCATGG 2 cut(s) 2116, 2475
NgoMIV GCCGGC 1 cut(s) 1186
NheI GCTAGC 1 cut(s) 109
NlaIV GGNNCC 5 cut(s) 654, 1565, 2293, 2332, 2473
NmuCI GTSAC 3 cut(s) 261, 368, 982
NsbI TGCGCA 1 cut(s) 1467
NspI RCATGY 3 cut(s) 146, 979, 1870
PaeI GCATGC 2 cut(s) 979, 1870
PagI TCATGA 1 cut(s) 502
PciI ACATGT 1 cut(s) 142
PctI GAATGC 2 cut(s) 329, 1322
PdiI GCCGGC 1 cut(s) 1188
PdmI GAANNNNTTC 1 cut(s) 2406
PfeI GAWTC 8 cut(s) 469, 770, 845, 1229, 1280, 1583, 1650, 1919
PleI GAGTC 3 cut(s) 268, 755, 1936
PpsI GAGTC 3 cut(s) 268, 755, 1936
Ppu21I YACGTR 1 cut(s) 805
PpuMI RGGWCCY 1 cut(s) 26
PscI ACATGT 1 cut(s) 142
PshBI ATTAAT 1 cut(s) 1673
PsiI TTATAA 1 cut(s) 2439
Psp5II RGGWCCY 1 cut(s) 26
Psp6I CCWGG 3 cut(s) 1267, 1504, 1891
PspEI GGTNACC 1 cut(s) 368
PspGI CCWGG 3 cut(s) 1267, 1504, 1891
PspN4I GGNNCC 5 cut(s) 654, 1565, 2293, 2332, 2473
PspPI GGNCC 6 cut(s) 26, 200, 956, 1577, 2292, 2354
PspPPI RGGWCCY 1 cut(s) 26
PstI CTGCAG 3 cut(s) 92, 1684, 1696
PstNI CAGNNNCTG 1 cut(s) 1691
PsuI RGATCY 4 cut(s) 1072, 1315, 1538, 2471
PvuII CAGCTG 2 cut(s) 1691, 2384
RsaI GTAC 4 cut(s) 1597, 2220, 2316, 2332
RsaNI GTAC 4 cut(s) 1596, 2219, 2315, 2331
RseI CAYNNNNRTG 1 cut(s) 2480
SalI GTCGAC 1 cut(s) 2109
SaqAI TTAA 9 cut(s) 524, 737, 1125, 1355, 1604, 1673, 1781, 2130, 2244
Sau96I GGNCC 6 cut(s) 26, 200, 956, 1577, 2292, 2354
ScaI AGTACT 2 cut(s) 1597, 2220
SchI GAGTC 3 cut(s) 269, 755, 1936
ScrFI CCNGG 4 cut(s) 798, 1269, 1506, 1893
SduI GDGCHC 3 cut(s) 150, 1822, 2091
SfcI CTRYAG 4 cut(s) 88, 1191, 1680, 1692
SinI GGWCC 4 cut(s) 26, 1577, 2292, 2354
SmiMI CAYNNNNRTG 1 cut(s) 2480
SmlI CTYRAG 3 cut(s) 783, 1997, 2180
SmoI CTYRAG 3 cut(s) 783, 1997, 2180
SphI GCATGC 2 cut(s) 979, 1870
SsiI CCGC 5 cut(s) 34, 268, 426, 1153, 1478
SspI AATATT 2 cut(s) 1240, 2394
StyD4I CCNGG 4 cut(s) 796, 1267, 1504, 1891
StyI CCWWGG 6 cut(s) 308, 443, 1345, 2116, 2295, 2475
TaaI ACNGT 6 cut(s) 379, 1081, 1563, 1711, 1786, 2176
TaiI ACGT 5 cut(s) 235, 807, 859, 1304, 2084
TaqI TCGA 4 cut(s) 562, 697, 2018, 2110
TatI WGTACW 2 cut(s) 1595, 2218
TauI GCSGC 3 cut(s) 37, 271, 429
TfiI GAWTC 8 cut(s) 469, 770, 845, 1229, 1280, 1583, 1650, 1919
Tru1I TTAA 9 cut(s) 524, 737, 1125, 1355, 1604, 1673, 1781, 2130, 2244
Tru9I TTAA 9 cut(s) 524, 737, 1125, 1355, 1604, 1673, 1781, 2130, 2244
TscAI CASTG 9 cut(s) 270, 382, 708, 1102, 1522, 1714, 1813, 2179, 2233
TseFI GTSAC 3 cut(s) 261, 368, 982
Tsp45I GTSAC 3 cut(s) 261, 368, 982
TspDTI ATGAA 7 cut(s) 17, 449, 519, 575, 1782, 2249, 2355
TspGWI ACGGA 2 cut(s) 57, 1895
TspRI CASTG 9 cut(s) 270, 382, 708, 1102, 1522, 1714, 1813, 2179, 2233
VpaK11BI GGWCC 4 cut(s) 26, 1577, 2292, 2354
VspI ATTAAT 1 cut(s) 1673
XapI RAATTY 1 cut(s) 2344
XceI RCATGY 3 cut(s) 146, 979, 1870
XmiI GTMKAC 1 cut(s) 2110
XmnI GAANNNNTTC 1 cut(s) 2406
ZrmI AGTACT 2 cut(s) 1597, 2220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.