Rorug03G0351600

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001434
Physical Location & Seq
Reverse (-)
43000588 .. 43002971
2384 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0351600.1

Sequence Viewer

Length: 687 bp
ATGGATGCAGAGAAGAAGGAATCGGACAACAGCCACAGCGAGAATCATAATAGCTATGCAAGAGCACTTATAGATTCTACCGCTCCTCCTACTACCGATAACACCAAGGAAGCCGACGACGAACGCGACCTCAGAGCTGGCCTCCATCCCCTCAAGAACAAATTCGTATTTTGGTACACTCGCCGAACACCAGGAATTCGAAGCCAGACGTCTTATGAAGACAACATAAAGAAAATTATCGATTTCAGCACGGTTGAAGGTTTTTGGGTGTGCTATTGCCACCTAGCCCGTCCTTCCTCACTGCCTAGCCCAACTGACTTGCATCTGTTCAAGGAGGGAATTCGCCCTCTTTGGGAGGACTCTGCTAATTGCAATGGTGGTAAATGGATAATACGATTCAAAAAGGTTGTCTCAGGCCGCTTTTGGGAGGACCTGGTTCTTGCCTTGGTGGGTGACCAACTTGACTATGGAGAGAACATATGTGGTGCTGTACTAAGCATTAGATTCAATGAGGATATAGTGAGTATCTGGAATCGTAATGCTTCTGATCATCAGGCTGTAATGGCTTTAAGAGATGCAATTAAGCGGAACTTGAAGCTTCCACACAGCTATGTCATGGAATACAAGCCTCATGATGCTTCACTCCGTGACAATTCATCATATCGAAACACTTGGTTGAGGGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04137 ko04140 map04137 map04140
Pfam Domains
Protein Families
WD40

Protein Analysis

228

Amino Acids

26.25

Weight (kDa)

6.71

Isoelectric Point (pI)

38.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IF4E PF01652 50 - 208 1.3e-55 Eukaryotic initiation factor 4E
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 212
AccBSI CCGCTC 1 cut(s) 83
AccII CGCG 1 cut(s) 126
AciI CCGC 3 cut(s) 81, 418, 586
AcsI RAATTY 3 cut(s) 161, 195, 339
AcyI GRCGYC 1 cut(s) 209
AdeI CACNNNGTG 1 cut(s) 647
AfaI GTAC 2 cut(s) 176, 492
AfiI CCNNNNNNNGG 2 cut(s) 352, 424
AgsI TTSAA 5 cut(s) 257, 331, 400, 508, 595
AjnI CCWGG 2 cut(s) 190, 432
AluBI AGCT 4 cut(s) 54, 137, 598, 609
AluI AGCT 4 cut(s) 54, 137, 598, 609
Alw21I GWGCWC 1 cut(s) 67
Alw26I GTCTC 1 cut(s) 415
AoxI GGCC 2 cut(s) 139, 415
ApoI RAATTY 3 cut(s) 161, 195, 339
ArsI GACNNNNNNTTYG 2 cut(s) 393, 425
Asp700I GAANNNNTTC 1 cut(s) 161
AspS9I GGNCC 1 cut(s) 430
AsuHPI GGTGA 1 cut(s) 464
AsuII TTCGAA 1 cut(s) 199
AvaII GGWCC 1 cut(s) 430
BbsI GAAGAC 1 cut(s) 225
Bbv12I GWGCWC 1 cut(s) 67
BccI CCATC 1 cut(s) 153
BciT130I CCWGG 2 cut(s) 192, 434
BclI TGATCA 1 cut(s) 547
BcoDI GTCTC 1 cut(s) 415
BfaI CTAG 2 cut(s) 284, 306
BisI GCNGC 1 cut(s) 418
BlsI GCNGC 1 cut(s) 419
Bme1390I CCNGG 2 cut(s) 192, 434
Bme18I GGWCC 1 cut(s) 430
BmgT120I GGNCC 1 cut(s) 430
BmrFI CCNGG 2 cut(s) 192, 434
BmsI GCATC 3 cut(s) 331, 565, 625
BpiI GAAGAC 1 cut(s) 225
BplI GAGNNNNNCTC 2 cut(s) 126, 158
Bpu14I TTCGAA 1 cut(s) 199
BpuEI CTTGAG 1 cut(s) 137
Bsa29I ATCGAT 1 cut(s) 240
BsaHI GRCGYC 1 cut(s) 209
BsaJI CCNNGG 2 cut(s) 105, 444
BsaXI ACNNNNNCTCC 2 cut(s) 70, 100
Bsc4I CCNNNNNNNGG 2 cut(s) 352, 424
Bse3DI GCAATG 1 cut(s) 379
BseBI CCWGG 2 cut(s) 192, 434
BseCI ATCGAT 1 cut(s) 240
BseDI CCNNGG 2 cut(s) 105, 444
BseGI GGATG 2 cut(s) 10, 145
BseLI CCNNNNNNNGG 2 cut(s) 352, 424
BseMI GCAATG 1 cut(s) 379
BseMII CTCAG 2 cut(s) 145, 426
BseRI GAGGAG 1 cut(s) 75
Bsh1236I CGCG 1 cut(s) 126
BshFI GGCC 2 cut(s) 141, 417
BshVI ATCGAT 1 cut(s) 240
BsiHKAI GWGCWC 1 cut(s) 67
BslI CCNNNNNNNGG 2 cut(s) 352, 424
BsmAI GTCTC 1 cut(s) 415
BsnI GGCC 2 cut(s) 141, 417
Bsp119I TTCGAA 1 cut(s) 199
Bsp1286I GDGCHC 1 cut(s) 67
Bsp143I GATC 1 cut(s) 547
BspACI CCGC 3 cut(s) 81, 418, 586
BspANI GGCC 2 cut(s) 141, 417
BspCNI CTCAG 2 cut(s) 144, 425
BspDI ATCGAT 1 cut(s) 240
BspFNI CGCG 1 cut(s) 126
BspHI TCATGA 1 cut(s) 631
BspT104I TTCGAA 1 cut(s) 199
BsrBI CCGCTC 1 cut(s) 83
BsrDI GCAATG 1 cut(s) 379
BssECI CCNNGG 2 cut(s) 105, 444
BssMI GATC 1 cut(s) 547
BssNI GRCGYC 1 cut(s) 209
BssT1I CCWWGG 2 cut(s) 105, 444
Bst2UI CCWGG 2 cut(s) 192, 434
Bst4CI ACNGT 1 cut(s) 253
BstACI GRCGYC 1 cut(s) 209
BstBI TTCGAA 1 cut(s) 199
BstC8I GCNNGC 1 cut(s) 139
BstDEI CTNAG 3 cut(s) 131, 412, 494
BstEII GGTNACC 1 cut(s) 452
BstF5I GGATG 2 cut(s) 10, 145
BstFNI CGCG 1 cut(s) 126
BstKTI GATC 1 cut(s) 550
BstMAI GTCTC 1 cut(s) 415
BstMBI GATC 1 cut(s) 547
BstMWI GCNNNNNNNGC 1 cut(s) 563
BstNI CCWGG 2 cut(s) 192, 434
BstPI GGTNACC 1 cut(s) 452
BstSCI CCNGG 2 cut(s) 190, 432
BstUI CGCG 1 cut(s) 126
BstV2I GAAGAC 1 cut(s) 225
Bsu15I ATCGAT 1 cut(s) 240
BsuRI GGCC 2 cut(s) 141, 417
BsuTUI ATCGAT 1 cut(s) 240
BtsCI GGATG 2 cut(s) 10, 145
BtsI GCAGTG 1 cut(s) 299
BtsIMutI CAGTG 1 cut(s) 299
Cac8I GCNNGC 1 cut(s) 139
CciI TCATGA 1 cut(s) 631
Cfr13I GGNCC 1 cut(s) 430
ClaI ATCGAT 1 cut(s) 240
CsiI ACCWGGT 1 cut(s) 432
Csp6I GTAC 2 cut(s) 175, 491
CviAII CATG 2 cut(s) 616, 632
CviQI GTAC 2 cut(s) 175, 491
DdeI CTNAG 3 cut(s) 131, 412, 494
DpnI GATC 1 cut(s) 549
DpnII GATC 1 cut(s) 547
DraIII CACNNNGTG 1 cut(s) 647
Eco130I CCWWGG 2 cut(s) 105, 444
Eco47I GGWCC 1 cut(s) 430
Eco91I GGTNACC 1 cut(s) 452
EcoO109I RGGNCCY 1 cut(s) 430
EcoO65I GGTNACC 1 cut(s) 452
EcoRI GAATTC 2 cut(s) 195, 339
EcoRII CCWGG 2 cut(s) 190, 432
EcoT14I CCWWGG 2 cut(s) 105, 444
ErhI CCWWGG 2 cut(s) 105, 444
FaeI CATG 2 cut(s) 619, 635
FalI AAGNNNNNCTT 2 cut(s) 575, 607
FatI CATG 2 cut(s) 615, 631
FauNDI CATATG 1 cut(s) 479
FbaI TGATCA 1 cut(s) 547
Fnu4HI GCNGC 1 cut(s) 418
FokI GGATG 2 cut(s) 17, 132
Fsp4HI GCNGC 1 cut(s) 418
FspBI CTAG 2 cut(s) 284, 306
GluI GCNGC 1 cut(s) 418
HaeIII GGCC 2 cut(s) 141, 417
Hin1I GRCGYC 1 cut(s) 209
Hin1II CATG 2 cut(s) 619, 635
HindIII AAGCTT 1 cut(s) 596
HinfI GANTC 7 cut(s) 20, 43, 74, 359, 396, 504, 532
HphI GGTGA 1 cut(s) 464
Hpy166II GTNNAC 1 cut(s) 177
Hpy188I TCNGA 3 cut(s) 25, 134, 547
Hpy188III TCNNGA 3 cut(s) 154, 529, 632
Hpy8I GTNNAC 1 cut(s) 177
Hpy99I CGWCG 2 cut(s) 119, 122
HpyAV CCTTC 3 cut(s) 10, 251, 303
HpyCH4III ACNGT 1 cut(s) 253
HpyCH4IV ACGT 1 cut(s) 209
HpyCH4V TGCA 5 cut(s) 8, 59, 322, 372, 578
HpyF10VI GCNNNNNNNGC 1 cut(s) 563
HpyF3I CTNAG 3 cut(s) 131, 412, 494
HpySE526I ACGT 1 cut(s) 209
Hsp92I GRCGYC 1 cut(s) 209
Hsp92II CATG 2 cut(s) 619, 635
Ksp22I TGATCA 1 cut(s) 547
Kzo9I GATC 1 cut(s) 547
LmnI GCTCC 1 cut(s) 88
LpnPI CCDG 9 cut(s) 123, 177, 204, 218, 399, 419, 446, 514, 539
LweI GCATC 3 cut(s) 331, 565, 625
MabI ACCWGGT 1 cut(s) 432
MaeI CTAG 2 cut(s) 284, 306
MaeII ACGT 1 cut(s) 209
MaeIII GTNAC 2 cut(s) 452, 647
MalI GATC 1 cut(s) 549
MbiI CCGCTC 1 cut(s) 83
MboI GATC 1 cut(s) 547
MboII GAAGA 2 cut(s) 25, 230
MhlI GDGCHC 1 cut(s) 67
MluCI AATT 7 cut(s) 161, 195, 234, 339, 367, 579, 652
MlyI GAGTC 1 cut(s) 353
MroXI GAANNNNTTC 1 cut(s) 161
MseI TTAA 2 cut(s) 569, 582
MslI CAYNNNNRTG 1 cut(s) 609
MspR9I CCNGG 2 cut(s) 192, 434
MvaI CCWGG 2 cut(s) 192, 434
MvnI CGCG 1 cut(s) 126
MwoI GCNNNNNNNGC 1 cut(s) 563
NdeI CATATG 1 cut(s) 479
NdeII GATC 1 cut(s) 547
NlaIII CATG 2 cut(s) 619, 635
NmuCI GTSAC 2 cut(s) 452, 647
NspV TTCGAA 1 cut(s) 199
PagI TCATGA 1 cut(s) 631
PcsI WCGNNNNNNNCGW 1 cut(s) 123
PdmI GAANNNNTTC 1 cut(s) 161
PfeI GAWTC 6 cut(s) 20, 43, 74, 396, 504, 532
PkrI GCNGC 1 cut(s) 419
PleI GAGTC 1 cut(s) 353
PpsI GAGTC 1 cut(s) 353
PpuMI RGGWCCY 1 cut(s) 430
Psp5II RGGWCCY 1 cut(s) 430
Psp6I CCWGG 2 cut(s) 190, 432
PspEI GGTNACC 1 cut(s) 452
PspGI CCWGG 2 cut(s) 190, 432
PspPI GGNCC 1 cut(s) 430
PspPPI RGGWCCY 1 cut(s) 430
RsaI GTAC 2 cut(s) 176, 492
RsaNI GTAC 2 cut(s) 175, 491
RseI CAYNNNNRTG 1 cut(s) 609
SaqAI TTAA 2 cut(s) 569, 582
SatI GCNGC 1 cut(s) 418
Sau3AI GATC 1 cut(s) 547
Sau96I GGNCC 1 cut(s) 430
SchI GAGTC 1 cut(s) 353
ScrFI CCNGG 2 cut(s) 192, 434
SduI GDGCHC 1 cut(s) 67
SexAI ACCWGGT 1 cut(s) 432
SfaNI GCATC 3 cut(s) 331, 565, 625
SfuI TTCGAA 1 cut(s) 199
SinI GGWCC 1 cut(s) 430
SmiMI CAYNNNNRTG 1 cut(s) 609
SmlI CTYRAG 1 cut(s) 152
SmoI CTYRAG 1 cut(s) 152
Sse9I AATT 7 cut(s) 161, 195, 234, 339, 367, 579, 652
SsiI CCGC 3 cut(s) 81, 418, 586
SspMI CTAG 2 cut(s) 284, 306
StyD4I CCNGG 2 cut(s) 190, 432
StyI CCWWGG 2 cut(s) 105, 444
TaaI ACNGT 1 cut(s) 253
TaiI ACGT 1 cut(s) 212
TaqI TCGA 3 cut(s) 199, 240, 664
TasI AATT 7 cut(s) 161, 195, 234, 339, 367, 579, 652
TatI WGTACW 1 cut(s) 490
TauI GCSGC 1 cut(s) 420
TfiI GAWTC 6 cut(s) 20, 43, 74, 396, 504, 532
Tru1I TTAA 2 cut(s) 569, 582
Tru9I TTAA 2 cut(s) 569, 582
TscAI CASTG 1 cut(s) 306
TseFI GTSAC 2 cut(s) 452, 647
Tsp45I GTSAC 2 cut(s) 452, 647
TspDTI ATGAA 2 cut(s) 231, 645
TspGWI ACGGA 1 cut(s) 635
TspRI CASTG 1 cut(s) 306
VpaK11BI GGWCC 1 cut(s) 430
XapI RAATTY 3 cut(s) 161, 195, 339
XcmI CCANNNNNNNNNTGG 1 cut(s) 464
XmnI GAANNNNTTC 1 cut(s) 161
XspI CTAG 2 cut(s) 284, 306
ZraI GACGTC 1 cut(s) 210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.