Rh6AG290000

WD domain, G-beta repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0001434
Physical Location & Seq
Forward (+)
48719897 .. 48727777
7881 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG290000.1

Sequence Viewer

Length: 2073 bp
ATGAAGTCCGATCTTATGATGACGGGGCCTTCGCCACCGTCCGATCAGTTCATGTCGCCGGCCGCAAGTCACCACTGCAGAAATGTGGTCGGCTTGATAGTAAGGAGAGAAATTTCTCCTCAAGCAAAACACTTGCCTAGAAGACGGTGGGGAGAAATTCCTAAAGTGAAAGCTGATTCCTCTTCCACTGGCCCTGAGTGCCAAGCAACAATAGATGCTAAGCGTGGCCTTCTTTCATGGGTTGAAGCAGAGTCACTACGGCATTTGTCAGCCAGGTACTGTAATTTAGTACCTCCTCCAAGGTCAACCATTGCAGCAGCATTTAGTCCCGATGGAAGAACTGTTGCTTCTACACATGGTGACCACACTGTGAAGATAATTGATTGCCAAACTGGAAGGTGCTTAAAGGTTTTGAGTGGCCATAGGAGGACCCCCTGGGTGGTGAGGTTCCACCCTTCGCAACCAGAAATAATTGCTAGTGGGGGTTTGGATCACGAAGTTCGCTTATGGGATCTAAACACTTCTGAGTGTATAGGATCACGTGATTTCCATCGACCTATTGCATCCATGGCTTTTCATGCAACAGGGGAACTCCTAGTTGTTGCATCAGGACACAAGGTGTATATATGGAACTACAAAAAGCAGCATTCGTCTCCAGACATTGTATTGAAAACATGGCGTTCACTTAGAGCAGTGCATTTTCACCCCCAAGCTGCTCCATTTCTTCTAACTGCTGAGGTCAATGATCTTGACTCTTCAGATTCCTTGATGACACCAGCAACATCTTCCGGCTACTTACAATATCCTTCTCCTGCTGTTTTCTTGGCAAACGTTCATTCTAGTGAACGCCTTAGTTTGGCTGCTGAACTGCCACATATGTCCTTGCCTTTCTTCTTTGTGTCACCATCTGTAGATAATCCAAGAAAAGAAATGCAGGGGGATGGGCATGTTGGTCCAAGTAACATGCAAGTCACTTCTGCTTTACGTCAGTTTGAAGCAGTTGCAAATGCAACAGAGCAGAGTGATAGCACAGCTTCTCCCATGGAGGCATTTCCATCAGTTATATCTCTTACTCATCTCAACGATGCCATGGAGACTGATGAGATGCAGGCTATAGGGAGAAGCCAGCATGAAAACTTTACTAATGTAGATTCTGCTATTAATAGACTACCTGAAAATATTCCGAATCCTCTTCATTCTGCTGAACCTCGGCAACTTCGTCATATTTCTCCTTATAGAGATCCAGCATTTTGGGAGCTTCCTTTTCTCCAAGGGTGGTTAACGGGTCAAAGCCAAGCCACTTTTCCCTCGATGCTTCCTCTTAATGGTGCTGGGCTTGACACTTCAGTCCAACATAATGGTTCTTCTAATTTAGCACCACATGTGGCAACTTCTTTAGCAATGCGAGGAAGCACCAGCGTATCCGGGGTTTCTGAAAGCGCTGGTTTGCATTTTCGCTTCTCAGTACCAGAATCTGGGGATGGTGCTGCTCTCTTAAATGCCCTGCATGATGGGAATGAAGCTCAGCCCATTATTAGCAGAATCCAGTCTGAAATTACTACATCACTGGCTGCTACAGCAGCTGCAGAGTTACCATGCACTGTGAAGCTACGAATATGGTCACACGACATAAAAAATCCTTCTGCTCCACTCAATACTGAACGATGCCGCTTAACCATTCCCCATGCTGTTCTTTGCAGTGAAATGGGCACTCATTTCTCTCCTTGTGGGAGATTCTTAGCCGCCTGTGTTGCATGCATGGTTCCTGATATGGATGCTGAACCTGGATTACAAAGTGTAGTCCATCAAGATTCTGGAATTGCAACATCCCCAACCCGGCATCCAATCTCAGCACATCAAGTCATTTATGAGCTTCGCATATATTCACTCGAGGAAGCAACATTTGGTTCAATACTCGCGTCCCGGGCTATCAGAGCTGCACATTGTTTGACTTCGATCCAGGTTAGTTTTATAGAAATCTTGGCTCCTATCTGCAATAGTTGTATTTATGGTCCGGGGATCTGGTGTTTTCTTTGTTGCTATTGCCTTGGATTCATTGTCTACTATGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000045 GO:0000422 GO:0000423 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0005856 GO:0005929 GO:0005930 GO:0006914 GO:0006950 GO:0006996 GO:0007005 GO:0007033 GO:0007275 GO:0007399 GO:0008150 GO:0008152 GO:0008285 GO:0009056 GO:0009790 GO:0009792 GO:0009888 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010506 GO:0010508 GO:0010562 GO:0010656 GO:0010660 GO:0010662 GO:0010664 GO:0010665 GO:0010667 GO:0010941 GO:0016043 GO:0016236 GO:0019216 GO:0019220 GO:0019222 GO:0019899 GO:0021915 GO:0022411 GO:0022607 GO:0030139 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0031410 GO:0031625 GO:0031982 GO:0032501 GO:0032502 GO:0032838 GO:0033554 GO:0033674 GO:0035295 GO:0042127 GO:0042325 GO:0042327 GO:0042981 GO:0042995 GO:0043009 GO:0043066 GO:0043067 GO:0043069 GO:0043085 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043523 GO:0043524 GO:0043549 GO:0043550 GO:0043551 GO:0043552 GO:0044085 GO:0044093 GO:0044237 GO:0044248 GO:0044389 GO:0044422 GO:0044424 GO:0044430 GO:0044441 GO:0044444 GO:0044446 GO:0044463 GO:0044464 GO:0045335 GO:0045834 GO:0045937 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051020 GO:0051174 GO:0051338 GO:0051347 GO:0051716 GO:0060429 GO:0060548 GO:0061726 GO:0061912 GO:0061919 GO:0065007 GO:0065009 GO:0070925 GO:0071840 GO:0080090 GO:0090218 GO:0097014 GO:0097708 GO:0098780 GO:0099568 GO:0120025 GO:0120038 GO:1901214 GO:1901215 GO:1903008 GO:1903725 GO:1903727 GO:1905037
KEGG Pathways
Metabolic & Signaling
ko04137 ko04140 map04137 map04140
Pfam Domains
Protein Families
WD40

Protein Analysis

690

Amino Acids

75.27

Weight (kDa)

6.36

Isoelectric Point (pI)

54.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Gbeta PF25391 104 - 211 1e-05 G protein beta WD-40 repeat protein
WDR55 PF24796 105 - 215 1.2e-09 WDR55
Beta-prop_THOC3 PF25174 106 - 244 1.1e-20 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 106 - 218 2.8e-14 WDR3 first beta-propeller domain
Beta-prop_WDR3_2nd PF25172 106 - 212 7.4e-07 WDR3 second beta-propeller domain
Beta-prop_WDR5 PF25175 107 - 248 8.3e-22 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 107 - 211 4.9e-11 WDHD1 first WD40 domain
WD40_CDC20-Fz PF24807 107 - 221 5.2e-14 CDC20/Fizzy WD40 domain
EIF3I PF24805 107 - 203 8.3e-06 EIF3I
WD40_Prp19 PF24814 108 - 237 1.3e-12 Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 109 - 216 2e-09 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR90_POC16_2nd PF23393 112 - 214 1.2e-06 WDR90/POC16, second beta-propeller
Beta-prop_EML_2 PF23414 146 - 236 1.1e-07 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1346
AccB7I CCANNNNNTGG 1 cut(s) 1475
AccI GTMKAC 1 cut(s) 2061
AccII CGCG 1 cut(s) 1919
AciI CCGC 3 cut(s) 63, 1669, 1743
AclI AACGTT 1 cut(s) 831
AclWI GGATC 6 cut(s) 498, 519, 544, 1235, 1951, 2027
AcoI YGGCCR 2 cut(s) 60, 418
AcsI RAATTY 2 cut(s) 111, 156
AcuI CTGAAG 2 cut(s) 741, 1329
AcvI CACGTG 1 cut(s) 542
AdeI CACNNNGTG 3 cut(s) 359, 370, 619
AfaI GTAC 3 cut(s) 278, 291, 1467
AfeI AGCGCT 1 cut(s) 1441
AfiI CCNNNNNNNGG 5 cut(s) 439, 856, 1325, 1475, 1836
AflIII ACRYGT 1 cut(s) 1381
AgsI TTSAA 4 cut(s) 245, 670, 995, 1911
AjnI CCWGG 4 cut(s) 272, 434, 1783, 1959
AjuI GAANNNNNNNTTGG 4 cut(s) 1287, 1319, 1887, 1919
AluBI AGCT 9 cut(s) 173, 713, 1034, 1258, 1523, 1583, 1609, 1873, 1937
AluI AGCT 9 cut(s) 173, 713, 1034, 1258, 1523, 1583, 1609, 1873, 1937
Alw26I GTCTC 2 cut(s) 657, 1088
AlwI GGATC 6 cut(s) 498, 519, 544, 1235, 1951, 2027
AlwNI CAGNNNCTG 3 cut(s) 279, 1475, 1583
Ama87I CYCGRG 2 cut(s) 1889, 1923
Aor51HI AGCGCT 1 cut(s) 1441
AoxI GGCC 5 cut(s) 26, 60, 190, 226, 418
ApoI RAATTY 2 cut(s) 111, 156
ArsI GACNNNNNNTTYG 2 cut(s) 13, 45
AseI ATTAAT 1 cut(s) 1161
Asp700I GAANNNNTTC 1 cut(s) 1179
AspLEI GCGC 1 cut(s) 1442
AspS9I GGNCC 5 cut(s) 26, 191, 429, 953, 2012
AsuC2I CCSGG 5 cut(s) 1426, 1837, 1924, 1925, 2016
AsuHPI GGTGA 5 cut(s) 62, 371, 454, 695, 894
AvaI CYCGRG 2 cut(s) 1889, 1923
AvaII GGWCC 3 cut(s) 429, 953, 2012
BaeGI GKGCMC 1 cut(s) 1712
BaeI ACNNNNGTAYC 2 cut(s) 1404, 1437
BalI TGGCCA 1 cut(s) 420
BbrPI CACGTG 1 cut(s) 542
BbsI GAAGAC 1 cut(s) 148
BbvCI CCTCAGC 1 cut(s) 735
BccI CCATC 8 cut(s) 326, 558, 913, 935, 1063, 1475, 1505, 1812
BceAI ACGGC 1 cut(s) 275
BciT130I CCWGG 4 cut(s) 274, 436, 1785, 1961
BciVI GTATCC 1 cut(s) 1432
BcnI CCSGG 5 cut(s) 1426, 1837, 1924, 1925, 2016
BcoDI GTCTC 2 cut(s) 657, 1088
BfaI CTAG 4 cut(s) 138, 477, 596, 840
BfmI CTRYAG 5 cut(s) 76, 909, 1113, 1575, 1584
BfoI RGCGCY 1 cut(s) 1443
BfuI GTATCC 1 cut(s) 1432
BlpI GCTNAGC 2 cut(s) 219, 1524
Bme1390I CCNGG 9 cut(s) 274, 436, 1426, 1785, 1837, 1924, 1925, 1961, 2016
Bme18I GGWCC 3 cut(s) 429, 953, 2012
BmeT110I CYCGRG 2 cut(s) 1889, 1923
BmgT120I GGNCC 5 cut(s) 26, 191, 429, 953, 2012
BmiI GGNNCC 5 cut(s) 27, 431, 449, 1764, 1986
BmrFI CCNGG 9 cut(s) 274, 436, 1426, 1785, 1837, 1924, 1925, 1961, 2016
BmsI GCATC 9 cut(s) 205, 572, 614, 1075, 1095, 1302, 1655, 1765, 1849
BpiI GAAGAC 1 cut(s) 148
BpmI CTGGAG 1 cut(s) 639
Bpu10I CCTNAGC 1 cut(s) 735
Bpu1102I GCTNAGC 2 cut(s) 219, 1524
BpuEI CTTGAG 1 cut(s) 105
BpuMI CCSGG 5 cut(s) 1426, 1837, 1924, 1925, 2016
BsaAI YACGTR 1 cut(s) 542
BsaBI GATNNNNATC 1 cut(s) 549
BsaXI ACNNNNNCTCC 2 cut(s) 1021, 1051
Bsc4I CCNNNNNNNGG 5 cut(s) 439, 856, 1325, 1475, 1836
Bse118I RCCGGY 1 cut(s) 58
Bse1I ACTGG 4 cut(s) 193, 397, 1546, 1572
Bse3DI GCAATG 2 cut(s) 309, 1407
Bse8I GATNNNNATC 1 cut(s) 549
BseBI CCWGG 4 cut(s) 274, 436, 1785, 1961
BseGI GGATG 6 cut(s) 563, 946, 1486, 1780, 1826, 1840
BseJI GATNNNNATC 1 cut(s) 549
BseLI CCNNNNNNNGG 5 cut(s) 439, 856, 1325, 1475, 1836
BseMI GCAATG 2 cut(s) 309, 1407
BseMII CTCAG 6 cut(s) 186, 516, 726, 1476, 1538, 1863
BseNI ACTGG 4 cut(s) 193, 397, 1546, 1572
BseRI GAGGAG 2 cut(s) 108, 285
BseSI GKGCMC 1 cut(s) 1712
BseX3I CGGCCG 1 cut(s) 60
BseYI CCCAGC 1 cut(s) 1331
BsgI GTGCAG 1 cut(s) 1923
Bsh1236I CGCG 1 cut(s) 1919
Bsh1285I CGRYCG 1 cut(s) 63
BshFI GGCC 5 cut(s) 28, 62, 192, 228, 420
BsiEI CGRYCG 1 cut(s) 63
BsiHKCI CYCGRG 2 cut(s) 1889, 1923
BsiSI CCGG 6 cut(s) 59, 789, 1425, 1837, 1924, 2015
BslFI GGGAC 2 cut(s) 312, 1906
BslI CCNNNNNNNGG 5 cut(s) 439, 856, 1325, 1475, 1836
BsmAI GTCTC 2 cut(s) 657, 1088
BsmBI CGTCTC 1 cut(s) 657
BsmFI GGGAC 2 cut(s) 312, 1906
BsmI GAATGC 1 cut(s) 646
BsnI GGCC 5 cut(s) 28, 62, 192, 228, 420
BsoBI CYCGRG 2 cut(s) 1889, 1923
Bsp1286I GDGCHC 1 cut(s) 1712
Bsp143I GATC 9 cut(s) 10, 43, 490, 511, 536, 745, 1240, 1956, 2019
Bsp1720I GCTNAGC 2 cut(s) 219, 1524
Bsp19I CCATGG 3 cut(s) 567, 1041, 1089
BspACI CCGC 3 cut(s) 63, 1669, 1743
BspANI GGCC 5 cut(s) 28, 62, 192, 228, 420
BspCNI CTCAG 6 cut(s) 187, 517, 727, 1475, 1537, 1862
BspFNI CGCG 1 cut(s) 1919
BspLI GGNNCC 5 cut(s) 27, 431, 449, 1764, 1986
BspMAI CTGCAG 2 cut(s) 80, 1588
BspPI GGATC 6 cut(s) 498, 519, 544, 1235, 1951, 2027
BsrDI GCAATG 2 cut(s) 309, 1407
BsrFI RCCGGY 1 cut(s) 58
BsrI ACTGG 4 cut(s) 193, 397, 1546, 1572
BssAI RCCGGY 1 cut(s) 58
BssMI GATC 9 cut(s) 10, 43, 490, 511, 536, 745, 1240, 1956, 2019
BssT1I CCWWGG 6 cut(s) 299, 567, 1041, 1089, 1270, 2047
Bst2UI CCWGG 4 cut(s) 274, 436, 1785, 1961
Bst4CI ACNGT 6 cut(s) 39, 147, 281, 343, 370, 1603
Bst6I CTCTTC 3 cut(s) 187, 760, 1197
BstBAI YACGTR 1 cut(s) 542
BstC8I GCNNGC 4 cut(s) 60, 1110, 1127, 1756
BstDSI CCRYGG 3 cut(s) 567, 1041, 1089
BstEII GGTNACC 1 cut(s) 359
BstF5I GGATG 6 cut(s) 563, 946, 1486, 1780, 1826, 1840
BstFNI CGCG 1 cut(s) 1919
BstH2I RGCGCY 1 cut(s) 1443
BstHHI GCGC 1 cut(s) 1442
BstKTI GATC 9 cut(s) 13, 46, 493, 514, 539, 748, 1243, 1959, 2022
BstMAI GTCTC 2 cut(s) 657, 1088
BstMBI GATC 9 cut(s) 10, 43, 490, 511, 536, 745, 1240, 1956, 2019
BstMCI CGRYCG 1 cut(s) 63
BstMWI GCNNNNNNNGC 8 cut(s) 198, 569, 578, 1577, 1580, 1751, 1925, 1934
BstNI CCWGG 4 cut(s) 274, 436, 1785, 1961
BstNSI RCATGY 4 cut(s) 950, 967, 1385, 1758
BstPI GGTNACC 1 cut(s) 359
BstSCI CCNGG 9 cut(s) 272, 434, 1424, 1783, 1835, 1922, 1923, 1959, 2014
BstSFI CTRYAG 5 cut(s) 76, 909, 1113, 1575, 1584
BstSLI GKGCMC 1 cut(s) 1712
BstUI CGCG 1 cut(s) 1919
BstV2I GAAGAC 1 cut(s) 148
BstX2I RGATCY 3 cut(s) 511, 1240, 2019
BstXI CCANNNNNNTGG 2 cut(s) 1251, 1358
BstYI RGATCY 3 cut(s) 511, 1240, 2019
BstZI CGGCCG 1 cut(s) 60
BsuI GTATCC 1 cut(s) 1432
BsuRI GGCC 5 cut(s) 28, 62, 192, 228, 420
BtgI CCRYGG 3 cut(s) 567, 1041, 1089
BtsCI GGATG 6 cut(s) 563, 946, 1486, 1780, 1826, 1840
BtsI GCAGTG 3 cut(s) 73, 699, 1705
BtsIMutI CAGTG 7 cut(s) 73, 186, 366, 699, 1565, 1599, 1705
Cac8I GCNNGC 4 cut(s) 60, 1110, 1127, 1756
CaiI CAGNNNCTG 3 cut(s) 279, 1475, 1583
CfoI GCGC 1 cut(s) 1442
Cfr10I RCCGGY 1 cut(s) 58
Cfr13I GGNCC 5 cut(s) 26, 191, 429, 953, 2012
Cfr9I CCCGGG 1 cut(s) 1923
CseI GACGC 1 cut(s) 1908
Csp6I GTAC 3 cut(s) 277, 290, 1466
CviQI GTAC 3 cut(s) 277, 290, 1466
DpnI GATC 9 cut(s) 12, 45, 492, 513, 538, 747, 1242, 1958, 2021
DpnII GATC 9 cut(s) 10, 43, 490, 511, 536, 745, 1240, 1956, 2019
DraIII CACNNNGTG 3 cut(s) 359, 370, 619
DrdI GACNNNNNNGTC 1 cut(s) 1346
DseDI GACNNNNNNGTC 1 cut(s) 1346
EaeI YGGCCR 2 cut(s) 60, 418
EagI CGGCCG 1 cut(s) 60
Eam1104I CTCTTC 3 cut(s) 187, 760, 1197
EarI CTCTTC 3 cut(s) 187, 760, 1197
EclXI CGGCCG 1 cut(s) 60
Eco130I CCWWGG 6 cut(s) 299, 567, 1041, 1089, 1270, 2047
Eco47I GGWCC 3 cut(s) 429, 953, 2012
Eco47III AGCGCT 1 cut(s) 1441
Eco52I CGGCCG 1 cut(s) 60
Eco57I CTGAAG 2 cut(s) 741, 1329
Eco72I CACGTG 1 cut(s) 542
Eco88I CYCGRG 2 cut(s) 1889, 1923
Eco91I GGTNACC 1 cut(s) 359
EcoO109I RGGNCCY 2 cut(s) 26, 429
EcoO65I GGTNACC 1 cut(s) 359
EcoRII CCWGG 4 cut(s) 272, 434, 1783, 1959
EcoT14I CCWWGG 6 cut(s) 299, 567, 1041, 1089, 1270, 2047
EcoT22I ATGCAT 1 cut(s) 1760
ErhI CCWWGG 6 cut(s) 299, 567, 1041, 1089, 1270, 2047
Esp3I CGTCTC 1 cut(s) 657
FaqI GGGAC 2 cut(s) 312, 1906
FauNDI CATATG 1 cut(s) 876
FblI GTMKAC 1 cut(s) 2061
FokI GGATG 6 cut(s) 550, 953, 1493, 1787, 1813, 1827
FspBI CTAG 4 cut(s) 138, 477, 596, 840
GlaI GCGC 1 cut(s) 1441
GsaI CCCAGC 1 cut(s) 1335
GsuI CTGGAG 1 cut(s) 639
HaeII RGCGCY 1 cut(s) 1443
HaeIII GGCC 5 cut(s) 28, 62, 192, 228, 420
HapII CCGG 6 cut(s) 59, 789, 1425, 1837, 1924, 2015
HgaI GACGC 1 cut(s) 1908
HhaI GCGC 1 cut(s) 1442
Hin6I GCGC 1 cut(s) 1440
HinP1I GCGC 1 cut(s) 1440
HincII GTYRAC 2 cut(s) 306, 1281
HindII GTYRAC 2 cut(s) 306, 1281
HpaI GTTAAC 1 cut(s) 1281
HpaII CCGG 6 cut(s) 59, 789, 1425, 1837, 1924, 2015
HphI GGTGA 5 cut(s) 62, 371, 454, 695, 894
Hpy166II GTNNAC 5 cut(s) 306, 683, 845, 1281, 2062
Hpy188I TCNGA 8 cut(s) 10, 43, 526, 760, 1185, 1435, 1552, 1934
Hpy188III TCNNGA 8 cut(s) 329, 494, 609, 656, 749, 1766, 1808, 1815
Hpy8I GTNNAC 5 cut(s) 306, 683, 845, 1281, 2062
HpyAV CCTTC 6 cut(s) 39, 239, 390, 465, 816, 1650
HpyCH4III ACNGT 6 cut(s) 39, 147, 281, 343, 370, 1603
HpyCH4IV ACGT 3 cut(s) 541, 831, 985
HpyF10VI GCNNNNNNNGC 8 cut(s) 198, 569, 578, 1577, 1580, 1751, 1925, 1934
HpySE526I ACGT 3 cut(s) 541, 831, 985
HspAI GCGC 1 cut(s) 1440
KroI GCCGGC 1 cut(s) 58
KroNI GCCGGC 1 cut(s) 60
KspAI GTTAAC 1 cut(s) 1281
Kzo9I GATC 9 cut(s) 10, 43, 490, 511, 536, 745, 1240, 1956, 2019
LmnI GCTCC 4 cut(s) 721, 1255, 1651, 1990
LweI GCATC 9 cut(s) 205, 572, 614, 1075, 1095, 1302, 1655, 1765, 1849
MaeI CTAG 4 cut(s) 138, 477, 596, 840
MaeII ACGT 3 cut(s) 541, 831, 985
MaeIII GTNAC 8 cut(s) 68, 252, 359, 900, 959, 970, 1590, 1620
MalI GATC 9 cut(s) 12, 45, 492, 513, 538, 747, 1242, 1958, 2021
MboI GATC 9 cut(s) 10, 43, 490, 511, 536, 745, 1240, 1956, 2019
MflI RGATCY 3 cut(s) 511, 1240, 2019
MhlI GDGCHC 1 cut(s) 1712
MlsI TGGCCA 1 cut(s) 420
MluCI AATT 8 cut(s) 111, 156, 283, 378, 471, 1369, 1554, 1818
MluNI TGGCCA 1 cut(s) 420
MlyI GAGTC 2 cut(s) 260, 746
MmeI TCCRAC 1 cut(s) 1375
Mox20I TGGCCA 1 cut(s) 420
Mph1103I ATGCAT 1 cut(s) 1760
MroNI GCCGGC 1 cut(s) 58
MroXI GAANNNNTTC 1 cut(s) 1179
MscI TGGCCA 1 cut(s) 420
MseI TTAA 6 cut(s) 404, 1161, 1280, 1323, 1496, 1673
MslI CAYNNNNRTG 1 cut(s) 840
Msp20I TGGCCA 1 cut(s) 420
MspA1I CMGCKG 1 cut(s) 1583
MspI CCGG 6 cut(s) 59, 789, 1425, 1837, 1924, 2015
MspR9I CCNGG 9 cut(s) 274, 436, 1426, 1785, 1837, 1924, 1925, 1961, 2016
Mva1269I GAATGC 1 cut(s) 646
MvaI CCWGG 4 cut(s) 274, 436, 1785, 1961
MvnI CGCG 1 cut(s) 1919
MwoI GCNNNNNNNGC 8 cut(s) 198, 569, 578, 1577, 1580, 1751, 1925, 1934
NaeI GCCGGC 1 cut(s) 60
NciI CCSGG 5 cut(s) 1426, 1837, 1924, 1925, 2016
NcoI CCATGG 3 cut(s) 567, 1041, 1089
NdeI CATATG 1 cut(s) 876
NdeII GATC 9 cut(s) 10, 43, 490, 511, 536, 745, 1240, 1956, 2019
NgoMIV GCCGGC 1 cut(s) 58
NlaIV GGNNCC 5 cut(s) 27, 431, 449, 1764, 1986
NmeAIII GCCGAG 1 cut(s) 1189
NmuCI GTSAC 6 cut(s) 68, 252, 359, 900, 970, 1620
NsiI ATGCAT 1 cut(s) 1760
NspI RCATGY 4 cut(s) 950, 967, 1385, 1758
PaeI GCATGC 1 cut(s) 1758
PaeR7I CTCGAG 1 cut(s) 1889
PasI CCCWGGG 1 cut(s) 435
PciI ACATGT 1 cut(s) 1381
PcsI WCGNNNNNNNCGW 1 cut(s) 1216
PctI GAATGC 1 cut(s) 646
PdiI GCCGGC 1 cut(s) 60
PdmI GAANNNNTTC 1 cut(s) 1179
PfeI GAWTC 9 cut(s) 176, 761, 1151, 1186, 1472, 1542, 1734, 1811, 2052
PflMI CCANNNNNTGG 1 cut(s) 1475
PleI GAGTC 2 cut(s) 259, 746
PmaCI CACGTG 1 cut(s) 542
PmlI CACGTG 1 cut(s) 542
PpsI GAGTC 2 cut(s) 259, 746
Ppu21I YACGTR 1 cut(s) 542
PpuMI RGGWCCY 1 cut(s) 429
PscI ACATGT 1 cut(s) 1381
PshBI ATTAAT 1 cut(s) 1161
Psp1406I AACGTT 1 cut(s) 831
Psp5II RGGWCCY 1 cut(s) 429
Psp6I CCWGG 4 cut(s) 272, 434, 1783, 1959
PspCI CACGTG 1 cut(s) 542
PspEI GGTNACC 1 cut(s) 359
PspFI CCCAGC 1 cut(s) 1331
PspGI CCWGG 4 cut(s) 272, 434, 1783, 1959
PspN4I GGNNCC 5 cut(s) 27, 431, 449, 1764, 1986
PspPI GGNCC 5 cut(s) 26, 191, 429, 953, 2012
PspPPI RGGWCCY 1 cut(s) 429
PspXI VCTCGAGB 1 cut(s) 1889
PsrI GAACNNNNNNTAC 2 cut(s) 1773, 1805
PstI CTGCAG 2 cut(s) 80, 1588
PstNI CAGNNNCTG 3 cut(s) 279, 1475, 1583
PsuI RGATCY 3 cut(s) 511, 1240, 2019
PvuII CAGCTG 1 cut(s) 1583
RsaI GTAC 3 cut(s) 278, 291, 1467
RsaNI GTAC 3 cut(s) 277, 290, 1466
RseI CAYNNNNRTG 1 cut(s) 840
SaqAI TTAA 6 cut(s) 404, 1161, 1280, 1323, 1496, 1673
Sau3AI GATC 9 cut(s) 10, 43, 490, 511, 536, 745, 1240, 1956, 2019
Sau96I GGNCC 5 cut(s) 26, 191, 429, 953, 2012
SchI GAGTC 2 cut(s) 260, 746
ScrFI CCNGG 9 cut(s) 274, 436, 1426, 1785, 1837, 1924, 1925, 1961, 2016
SduI GDGCHC 1 cut(s) 1712
SfaNI GCATC 9 cut(s) 205, 572, 614, 1075, 1095, 1302, 1655, 1765, 1849
SfcI CTRYAG 5 cut(s) 76, 909, 1113, 1575, 1584
Sfr274I CTCGAG 1 cut(s) 1889
SinI GGWCC 3 cut(s) 429, 953, 2012
SlaI CTCGAG 1 cut(s) 1889
SmaI CCCGGG 1 cut(s) 1925
SmiMI CAYNNNNRTG 1 cut(s) 840
SmlI CTYRAG 2 cut(s) 120, 1889
SmoI CTYRAG 2 cut(s) 120, 1889
SphI GCATGC 1 cut(s) 1758
Sse9I AATT 8 cut(s) 111, 156, 283, 378, 471, 1369, 1554, 1818
SsiI CCGC 3 cut(s) 63, 1669, 1743
SspI AATATT 1 cut(s) 1180
SspMI CTAG 4 cut(s) 138, 477, 596, 840
StyD4I CCNGG 9 cut(s) 272, 434, 1424, 1783, 1835, 1922, 1923, 1959, 2014
StyI CCWWGG 6 cut(s) 299, 567, 1041, 1089, 1270, 2047
TaaI ACNGT 6 cut(s) 39, 147, 281, 343, 370, 1603
TaiI ACGT 3 cut(s) 544, 834, 988
TaqI TCGA 4 cut(s) 553, 1310, 1890, 1955
TasI AATT 8 cut(s) 111, 156, 283, 378, 471, 1369, 1554, 1818
TauI GCSGC 3 cut(s) 65, 1671, 1745
TfiI GAWTC 9 cut(s) 176, 761, 1151, 1186, 1472, 1542, 1734, 1811, 2052
Tru1I TTAA 6 cut(s) 404, 1161, 1280, 1323, 1496, 1673
Tru9I TTAA 6 cut(s) 404, 1161, 1280, 1323, 1496, 1673
TscAI CASTG 7 cut(s) 80, 193, 373, 699, 1572, 1606, 1705
TseFI GTSAC 6 cut(s) 68, 252, 359, 900, 970, 1620
Tsp45I GTSAC 6 cut(s) 68, 252, 359, 900, 970, 1620
TspDTI ATGAA 9 cut(s) 17, 40, 225, 566, 824, 1146, 1184, 1533, 2044
TspMI CCCGGG 1 cut(s) 1923
TspRI CASTG 7 cut(s) 80, 193, 373, 699, 1572, 1606, 1705
Van91I CCANNNNNTGG 1 cut(s) 1475
VpaK11BI GGWCC 3 cut(s) 429, 953, 2012
VspI ATTAAT 1 cut(s) 1161
XapI RAATTY 2 cut(s) 111, 156
XceI RCATGY 4 cut(s) 950, 967, 1385, 1758
XcmI CCANNNNNNNNNTGG 1 cut(s) 1811
XhoI CTCGAG 1 cut(s) 1889
XmaI CCCGGG 1 cut(s) 1923
XmiI GTMKAC 1 cut(s) 2061
XmnI GAANNNNTTC 1 cut(s) 1179
XspI CTAG 4 cut(s) 138, 477, 596, 840
Zsp2I ATGCAT 1 cut(s) 1760
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.