MD13G1226100.v1.1

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0001434
Physical Location & Seq
Reverse (-)
22095323 .. 22102432
7110 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1226100.v1.1.491

Sequence Viewer

Length: 2304 bp
ATGAGGGCAGGTTCTTTGTGGGCCGACGAGCCCAATTCTCCGTTCACCTCTGTCCCGCGCGTACCTCAGCCCAATCCGTGCAAGCACAGTGGTAGCAATGTTCTGAGACTGTTAGCACAGAGAGAGGTTTCTCCGCAGACGAAACATTGGTCAAAGAAGCTATGGGGGGAACCTTCTAAGTCTAATTCTGATTCCGTTGGCCCAAAGTTTGAAGCAGCTAGAGATGCTAGACACAGCCTTATCTCATGGGTGGAGGCAGAGTCACTCCAGCATTTGTCTGCCAAATATTGTCCGCTTGTGCCTCCTCCACGGTCAACTATTGCAGCAGCCTTCAGCCCTGATGGAAGGACACTGGCCTCAACTCACGGAGACCATACGGTTAAGATTATTGATTGCCAAACTGGGATTTGCTTAAAGGTCTTGAGTGGTCACCGTAGGACACCGTGGGTGGTTAGGTTTCATCCCTTGTATCCAGACATATTGGCTAGTGGAAGTTTGGATCACGAAGTTCGCTTGTGGGATGCAAAAACGGCAGAGTGTTTAGGATCTCGTGATTTCTTGCGTCCTATTGCTTCCATAGCTTTCCATGCCCAAGGTGAGCTGCTTGCAGTTGCGTCAGGTCACAAGTTGTACATATGGCACTACAATAGGAGAGGGGAAACATCCTCCCCAACCATAGTACTGAAGACACGGCGTTCGCTCCGGGCTGTGCATTTTCACCCTCATGGAGCTCCTTTCCTTTTAACTGCTGAGGTGAATGACCTTGACTCCTCAGATTCCTCGATGACACTTGCAACTTCTCTGGGTTATTTGCGCTATCCTCCACCTACTGTATATTTGGCAGATGGTCAATCTAGTGATCGTTCTGGTTTGGTAGATGGACTACCTCTTATGTCTTTACCATTTCTGATATGGCCTTCATTTGATAGAGATAATGGGAGAATATCTATGCAGCGTAGTAACGTGGACATTGGTTCAAGTAGTGCGCTACAGCGAGTTGATCCTTCTGCTTCAGTGCGCTTGCTAACATATTCAACTCCTTCAGGGCAGTATGAACTTCTGCTGTCCCCAATTGAACCTAGTAGCTCTTCTCCAGCACCAGAAGAGACAGAAACTAATACTTTCTTGAATGAAATGGAAACTGAAGTTTCTCAACCTGCAGTGGACAGTTTAGAGGCTATGGAAGTGCAGCCTGAAGGGAGGAGCAATCATATTTTCCCATTTGGTGACTCAACATATTGGGAACTTCCTTTCTTGCAAGGGTGGTTAATTGGTCAGACCCAAGCTAGCCAACGGAATATGCGGCCGGTAAGTGATGCTACCCAAGATAATCCATCTGCACATGGTGAGATGGATAATCCAGCCATAACTTCTTCAGTAATACCAACCGGTGTGAACCAATCCAGGGTTACTGGAAGATCTAGTACCCGGCATCGTACTTCAAGAACTCATATGGTGTCGACAATTGGATCTAATGAAGGTGCTGGATTCAATAGTATTCCGCATGCTGAAAGTGAGCCTCAACCTGTTGTGAGCAGAATCCAATCTGAACTTGCCAACTCACTCGCTGCAGCAGCAGCTGCAGAGTTGCCTTGCACTGTGAAGCTCAGAATTTGGCCACATGATTTGAAAAATCCTTGTTCTCCCCTTGATGCAGAAAGATGTCGCTTAACCATTCCACATGCTGTACTTTGTAGTGAGATGGGTGCCCATTTTTCCCCTTGCGGAAGGTTTTTAGCTGCATGTGTTGCATGTATGTTACCCCATATGGAAGCTGATCTTGGATTGCAGAGCCAGGTCAACCATGACGTGACAGGGGCATCAACTTCCCCAACTCGACATCCAATATCAGCTCACCATGTCGTGTATGAGCTCCGGATATATTCCCTTGAGGAAGCAACATTTGGAACGGTTCTTGCATCCCGGGCAATAAGAGCTGCCCACTGCCTAACATCTATTCAGTTCTCTCCTACGTCAGAGCATATATTACTTGCCTATGGTCGTCGTCACAGTTCACTTCTTAAGAGTGTTGTCATTGATGGAGAGACTACAGTGCCTATTTACACCATTCTGGAGGTCTACAGAATTTCTGATATGGAACTTGTGAGAGTTCTTCCTAGTGCAGAGGATGAGGTCAATGTAGCTTGCTTTCATCCTTCAGTTGGAGGTGGCCTCGTCTATGGAACCAAGGAAGGGAAGTTAAGGATCCTCCAATATGATAGTTTTCATGGGACGAATCAGACAACATCCGCTTTTCTTGATGAAAACATGCTTGAGGTTCCGACATATGCCTTGGAATGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04137 ko04140 map04137 map04140
Pfam Domains
Protein Families
WD40

Protein Analysis

768

Amino Acids

83.98

Weight (kDa)

6.16

Isoelectric Point (pI)

52.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 108 - 213 1.2e-11 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 109 - 243 7.3e-20 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 110 - 253 3e-20 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 110 - 217 1.1e-09 WDHD1 first WD40 domain
WD40_CDC20-Fz PF24807 110 - 218 9e-11 CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 111 - 216 9.1e-06 WDR36/Utp21 second beta-propeller domain
WD40_Prp19 PF24814 114 - 244 7.1e-11 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 149 - 244 2.4e-06 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1165
AccB1I GGYRCC 1 cut(s) 1706
AccI GTMKAC 2 cut(s) 1460, 2079
AccII CGCG 2 cut(s) 58, 60
AccIII TCCGGA 1 cut(s) 1875
AciI CCGC 7 cut(s) 56, 134, 293, 1303, 1502, 1725, 2250
AclWI GGATC 6 cut(s) 507, 553, 995, 1477, 2200, 2213
AcoI YGGCCR 2 cut(s) 1304, 1616
AcsI RAATTY 2 cut(s) 1611, 2085
AcuI CTGAAG 8 cut(s) 316, 704, 996, 1026, 1164, 1215, 1359, 2142
AdeI CACNNNGTG 1 cut(s) 1346
AfaI GTAC 6 cut(s) 63, 632, 681, 1426, 1438, 1689
AfiI CCNNNNNNNGG 3 cut(s) 1405, 2162, 2193
AflII CTTAAG 1 cut(s) 2021
AgeI ACCGGT 1 cut(s) 1388
AgsI TTSAA 8 cut(s) 212, 978, 1035, 1076, 1129, 1443, 1492, 1630
AjiI CACGTC 1 cut(s) 1810
AjnI CCWGG 2 cut(s) 1403, 1794
AjuI GAANNNNNNNTTGG 6 cut(s) 26, 58, 1764, 1796, 1887, 1919
Alw21I GWGCWC 2 cut(s) 733, 1875
Alw26I GTCTC 4 cut(s) 100, 363, 1100, 2039
AlwI GGATC 6 cut(s) 507, 553, 995, 1477, 2200, 2213
AlwNI CAGNNNCTG 1 cut(s) 1580
Ama87I CYCGRG 1 cut(s) 1923
Aor13HI TCCGGA 1 cut(s) 1875
AoxI GGCC 7 cut(s) 21, 199, 354, 914, 1304, 1616, 2170
ApoI RAATTY 2 cut(s) 1611, 2085
ArsI GACNNNNNNTTYG 4 cut(s) 134, 166, 679, 711
AsiGI ACCGGT 1 cut(s) 1388
AspLEI GCGC 4 cut(s) 60, 816, 988, 1020
AspS9I GGNCC 2 cut(s) 21, 200
AsuC2I CCSGG 4 cut(s) 704, 1429, 1924, 1925
AsuHPI GGTGA 8 cut(s) 37, 422, 608, 710, 766, 1238, 1358, 1847
AsuNHI GCTAGC 1 cut(s) 1286
AvaI CYCGRG 1 cut(s) 1923
BaeGI GKGCMC 1 cut(s) 1711
BalI TGGCCA 1 cut(s) 1618
BamHI GGATCC 1 cut(s) 2205
BanI GGYRCC 1 cut(s) 1706
BanII GRGCYC 3 cut(s) 33, 733, 1875
BarI GAAGNNNNNNTAC 2 cut(s) 1408, 1440
BauI CACGAG 1 cut(s) 549
BbsI GAAGAC 1 cut(s) 692
Bbv12I GWGCWC 2 cut(s) 733, 1875
BbvCI CCTCAGC 2 cut(s) 66, 750
BccI CCATC 7 cut(s) 335, 839, 872, 1342, 1345, 1696, 2033
BceAI ACGGC 2 cut(s) 546, 707
BciT130I CCWGG 2 cut(s) 1405, 1796
BciVI GTATCC 1 cut(s) 480
BcnI CCSGG 4 cut(s) 704, 1429, 1924, 1925
BcoDI GTCTC 4 cut(s) 100, 363, 1100, 2039
BfaI CTAG 9 cut(s) 219, 228, 486, 855, 1080, 1287, 1422, 2118, 2302
BfmI CTRYAG 6 cut(s) 989, 1158, 1569, 1581, 2049, 2080
BfrI CTTAAG 1 cut(s) 2021
BfuAI ACCTGC 1 cut(s) 1165
BfuI GTATCC 1 cut(s) 480
BglII AGATCT 1 cut(s) 1418
BmcAI AGTACT 1 cut(s) 681
Bme1390I CCNGG 6 cut(s) 704, 1405, 1429, 1796, 1924, 1925
BmeT110I CYCGRG 1 cut(s) 1923
BmgBI CACGTC 1 cut(s) 1810
BmgT120I GGNCC 2 cut(s) 21, 200
BmiI GGNNCC 5 cut(s) 171, 1708, 2185, 2207, 2280
BmrFI CCNGG 6 cut(s) 704, 1405, 1429, 1796, 1924, 1925
BmrI ACTGGG 1 cut(s) 411
BmsI GCATC 7 cut(s) 214, 511, 1306, 1441, 1642, 1829, 1928
BmtI GCTAGC 1 cut(s) 1290
BmuI ACTGGG 1 cut(s) 411
BpiI GAAGAC 1 cut(s) 692
BplI GAGNNNNNCTC 2 cut(s) 2157, 2189
BpmI CTGGAG 3 cut(s) 251, 1077, 2093
Bpu10I CCTNAGC 2 cut(s) 66, 750
BpuEI CTTGAG 3 cut(s) 442, 1910, 2294
BpuMI CCSGG 4 cut(s) 704, 1429, 1924, 1925
BsaI GGTCTC 1 cut(s) 363
BsaJI CCNNGG 7 cut(s) 308, 443, 592, 1404, 1923, 2187, 2292
BsaWI WCCGGW 2 cut(s) 1388, 1875
BsaXI ACNNNNNCTCC 4 cut(s) 245, 275, 752, 782
Bsc4I CCNNNNNNNGG 3 cut(s) 1405, 2162, 2193
Bse118I RCCGGY 2 cut(s) 1306, 1388
Bse1I ACTGG 3 cut(s) 357, 406, 1417
Bse3DI GCAATG 1 cut(s) 103
BseAI TCCGGA 1 cut(s) 1875
BseBI CCWGG 2 cut(s) 1405, 1796
BseDI CCNNGG 7 cut(s) 308, 443, 592, 1404, 1923, 2187, 2292
BseGI GGATG 8 cut(s) 460, 526, 662, 1840, 1919, 2134, 2152, 2246
BseLI CCNNNNNNNGG 3 cut(s) 1405, 2162, 2193
BseMI GCAATG 1 cut(s) 103
BseMII CTCAG 5 cut(s) 80, 95, 741, 786, 1621
BseNI ACTGG 3 cut(s) 357, 406, 1417
BseRI GAGGAG 3 cut(s) 294, 760, 1216
BseSI GKGCMC 1 cut(s) 1711
BseX3I CGGCCG 1 cut(s) 1304
BsgI GTGCAG 3 cut(s) 1208, 1323, 2142
Bsh1236I CGCG 2 cut(s) 58, 60
Bsh1285I CGRYCG 1 cut(s) 1307
BshFI GGCC 7 cut(s) 23, 201, 356, 916, 1306, 1618, 2172
BshNI GGYRCC 1 cut(s) 1706
BshTI ACCGGT 1 cut(s) 1388
BsiEI CGRYCG 1 cut(s) 1307
BsiHKAI GWGCWC 2 cut(s) 733, 1875
BsiHKCI CYCGRG 1 cut(s) 1923
BsiSI CCGG 6 cut(s) 703, 1307, 1389, 1429, 1876, 1924
BslFI GGGAC 3 cut(s) 38, 1051, 2245
BslI CCNNNNNNNGG 3 cut(s) 1405, 2162, 2193
BsmAI GTCTC 4 cut(s) 100, 363, 1100, 2039
BsmFI GGGAC 3 cut(s) 38, 1051, 2245
BsmI GAATGC 1 cut(s) 2303
BsnI GGCC 7 cut(s) 23, 201, 356, 916, 1306, 1618, 2172
Bso31I GGTCTC 1 cut(s) 363
BsoBI CYCGRG 1 cut(s) 1923
Bsp1286I GDGCHC 4 cut(s) 33, 733, 1711, 1875
Bsp13I TCCGGA 1 cut(s) 1875
Bsp1407I TGTACA 1 cut(s) 630
Bsp143I GATC 8 cut(s) 499, 545, 859, 1000, 1418, 1469, 1777, 2205
BspACI CCGC 7 cut(s) 56, 134, 293, 1303, 1502, 1725, 2250
BspANI GGCC 7 cut(s) 23, 201, 356, 916, 1306, 1618, 2172
BspCNI CTCAG 5 cut(s) 79, 96, 742, 785, 1620
BspEI TCCGGA 1 cut(s) 1875
BspFNI CGCG 2 cut(s) 58, 60
BspLI GGNNCC 5 cut(s) 171, 1708, 2185, 2207, 2280
BspMAI CTGCAG 3 cut(s) 1162, 1573, 1585
BspMI ACCTGC 1 cut(s) 1165
BspOI GCTAGC 1 cut(s) 1290
BspPI GGATC 6 cut(s) 507, 553, 995, 1477, 2200, 2213
BspQI GCTCTTC 1 cut(s) 1093
BspT107I GGYRCC 1 cut(s) 1706
BspTI CTTAAG 1 cut(s) 2021
BspTNI GGTCTC 1 cut(s) 363
BsrDI GCAATG 1 cut(s) 103
BsrFI RCCGGY 2 cut(s) 1306, 1388
BsrGI TGTACA 1 cut(s) 630
BsrI ACTGG 3 cut(s) 357, 406, 1417
BssAI RCCGGY 2 cut(s) 1306, 1388
BssECI CCNNGG 7 cut(s) 308, 443, 592, 1404, 1923, 2187, 2292
BssMI GATC 8 cut(s) 499, 545, 859, 1000, 1418, 1469, 1777, 2205
BssSI CACGAG 1 cut(s) 549
BssT1I CCWWGG 3 cut(s) 592, 2187, 2292
Bst2BI CACGAG 1 cut(s) 549
Bst2UI CCWGG 2 cut(s) 1405, 1796
Bst6I CTCTTC 2 cut(s) 1093, 1098
BstAFI CTTAAG 1 cut(s) 2021
BstAPI GCANNNNNTGC 2 cut(s) 1580, 1748
BstAUI TGTACA 1 cut(s) 630
BstC8I GCNNGC 6 cut(s) 83, 606, 1022, 1288, 1506, 2146
BstDEI CTNAG 6 cut(s) 66, 104, 177, 750, 772, 1607
BstDSI CCRYGG 2 cut(s) 308, 443
BstEII GGTNACC 1 cut(s) 428
BstF5I GGATG 8 cut(s) 460, 526, 662, 1840, 1919, 2134, 2152, 2246
BstFNI CGCG 2 cut(s) 58, 60
BstHHI GCGC 4 cut(s) 60, 816, 988, 1020
BstKTI GATC 8 cut(s) 502, 548, 862, 1003, 1421, 1472, 1780, 2208
BstMAI GTCTC 4 cut(s) 100, 363, 1100, 2039
BstMBI GATC 8 cut(s) 499, 545, 859, 1000, 1418, 1469, 1777, 2205
BstMCI CGRYCG 1 cut(s) 1307
BstNI CCWGG 2 cut(s) 1405, 1796
BstNSI RCATGY 5 cut(s) 1508, 1685, 1746, 1755, 2272
BstPI GGTNACC 1 cut(s) 428
BstSCI CCNGG 6 cut(s) 702, 1403, 1427, 1794, 1922, 1923
BstSFI CTRYAG 6 cut(s) 989, 1158, 1569, 1581, 2049, 2080
BstSLI GKGCMC 1 cut(s) 1711
BstUI CGCG 2 cut(s) 58, 60
BstV2I GAAGAC 1 cut(s) 692
BstX2I RGATCY 4 cut(s) 545, 1418, 1469, 2205
BstYI RGATCY 4 cut(s) 545, 1418, 1469, 2205
BstZI CGGCCG 1 cut(s) 1304
BsuI GTATCC 1 cut(s) 480
BsuRI GGCC 7 cut(s) 23, 201, 356, 916, 1306, 1618, 2172
BtgI CCRYGG 2 cut(s) 308, 443
BtrI CACGTC 1 cut(s) 1810
BtsCI GGATG 8 cut(s) 460, 526, 662, 1840, 1919, 2134, 2152, 2246
BtsI GCAGTG 2 cut(s) 1167, 1942
BtsIMutI CAGTG 7 cut(s) 94, 350, 1020, 1167, 1596, 1942, 2058
BveI ACCTGC 1 cut(s) 1165
Cac8I GCNNGC 6 cut(s) 83, 606, 1022, 1288, 1506, 2146
CaiI CAGNNNCTG 1 cut(s) 1580
CfoI GCGC 4 cut(s) 60, 816, 988, 1020
Cfr10I RCCGGY 2 cut(s) 1306, 1388
Cfr13I GGNCC 2 cut(s) 21, 200
Cfr9I CCCGGG 1 cut(s) 1923
CseI GACGC 2 cut(s) 551, 603
Csp6I GTAC 6 cut(s) 62, 631, 680, 1425, 1437, 1688
CspAI ACCGGT 1 cut(s) 1388
CspCI CAANNNNNGTGG 4 cut(s) 70, 105, 1608, 1643
CviQI GTAC 6 cut(s) 62, 631, 680, 1425, 1437, 1688
DdeI CTNAG 6 cut(s) 66, 104, 177, 750, 772, 1607
DpnI GATC 8 cut(s) 501, 547, 861, 1002, 1420, 1471, 1779, 2207
DpnII GATC 8 cut(s) 499, 545, 859, 1000, 1418, 1469, 1777, 2205
DraIII CACNNNGTG 1 cut(s) 1346
EaeI YGGCCR 2 cut(s) 1304, 1616
EagI CGGCCG 1 cut(s) 1304
Eam1104I CTCTTC 2 cut(s) 1093, 1098
EarI CTCTTC 2 cut(s) 1093, 1098
Ecl136II GAGCTC 2 cut(s) 731, 1873
EclXI CGGCCG 1 cut(s) 1304
Eco130I CCWWGG 3 cut(s) 592, 2187, 2292
Eco24I GRGCYC 3 cut(s) 33, 733, 1875
Eco31I GGTCTC 1 cut(s) 363
Eco52I CGGCCG 1 cut(s) 1304
Eco53kI GAGCTC 2 cut(s) 731, 1873
Eco57I CTGAAG 8 cut(s) 316, 704, 996, 1026, 1164, 1215, 1359, 2142
Eco88I CYCGRG 1 cut(s) 1923
Eco91I GGTNACC 1 cut(s) 428
EcoICRI GAGCTC 2 cut(s) 731, 1873
EcoO65I GGTNACC 1 cut(s) 428
EcoRII CCWGG 2 cut(s) 1403, 1794
EcoT14I CCWWGG 3 cut(s) 592, 2187, 2292
EcoT38I GRGCYC 3 cut(s) 33, 733, 1875
ErhI CCWWGG 3 cut(s) 592, 2187, 2292
FalI AAGNNNNNCTT 2 cut(s) 1764, 1796
FaqI GGGAC 3 cut(s) 38, 1051, 2245
FauI CCCGC 1 cut(s) 63
FauNDI CATATG 4 cut(s) 635, 1452, 1767, 2287
FblI GTMKAC 2 cut(s) 1460, 2079
FokI GGATG 8 cut(s) 447, 533, 649, 1827, 1906, 2139, 2141, 2233
FriOI GRGCYC 3 cut(s) 33, 733, 1875
FspBI CTAG 9 cut(s) 219, 228, 486, 855, 1080, 1287, 1422, 2118, 2302
GlaI GCGC 4 cut(s) 59, 815, 987, 1019
GsuI CTGGAG 3 cut(s) 251, 1077, 2093
HaeIII GGCC 7 cut(s) 23, 201, 356, 916, 1306, 1618, 2172
HapII CCGG 6 cut(s) 703, 1307, 1389, 1429, 1876, 1924
HgaI GACGC 2 cut(s) 551, 603
HhaI GCGC 4 cut(s) 60, 816, 988, 1020
Hin6I GCGC 4 cut(s) 58, 814, 986, 1018
HinP1I GCGC 4 cut(s) 58, 814, 986, 1018
HincII GTYRAC 3 cut(s) 315, 1461, 1801
HindII GTYRAC 3 cut(s) 315, 1461, 1801
HinfI GANTC 8 cut(s) 191, 260, 767, 776, 1229, 1488, 1539, 2236
HpaII CCGG 6 cut(s) 703, 1307, 1389, 1429, 1876, 1924
HphI GGTGA 8 cut(s) 37, 422, 608, 710, 766, 1238, 1358, 1847
Hpy166II GTNNAC 9 cut(s) 45, 315, 967, 1165, 1396, 1461, 1801, 2015, 2080
Hpy188III TCNNGA 9 cut(s) 421, 473, 503, 551, 1126, 1443, 1876, 2072, 2258
Hpy8I GTNNAC 9 cut(s) 45, 315, 967, 1165, 1396, 1461, 1801, 2015, 2080
Hpy99I CGWCG 2 cut(s) 29, 2007
HpyCH4IV ACGT 3 cut(s) 963, 1809, 1973
HpyF3I CTNAG 6 cut(s) 66, 104, 177, 750, 772, 1607
HpySE526I ACGT 3 cut(s) 963, 1809, 1973
HspAI GCGC 4 cut(s) 58, 814, 986, 1018
Kpn2I TCCGGA 1 cut(s) 1875
Kzo9I GATC 8 cut(s) 499, 545, 859, 1000, 1418, 1469, 1777, 2205
LguI GCTCTTC 1 cut(s) 1093
LmnI GCTCC 5 cut(s) 705, 728, 736, 1203, 1878
LweI GCATC 7 cut(s) 214, 511, 1306, 1441, 1642, 1829, 1928
MaeI CTAG 9 cut(s) 219, 228, 486, 855, 1080, 1287, 1422, 2118, 2302
MaeII ACGT 3 cut(s) 963, 1809, 1973
MaeIII GTNAC 9 cut(s) 261, 428, 620, 959, 1226, 1408, 1758, 1810, 2006
MalI GATC 8 cut(s) 501, 547, 861, 1002, 1420, 1471, 1779, 2207
MboI GATC 8 cut(s) 499, 545, 859, 1000, 1418, 1469, 1777, 2205
MboII GAAGA 6 cut(s) 697, 1080, 1115, 1365, 1428, 2105
MfeI CAATTG 2 cut(s) 1071, 1464
MflI RGATCY 4 cut(s) 545, 1418, 1469, 2205
MhlI GDGCHC 4 cut(s) 33, 733, 1711, 1875
MlsI TGGCCA 1 cut(s) 1618
MluCI AATT 7 cut(s) 34, 184, 1071, 1269, 1464, 1611, 2085
MluNI TGGCCA 1 cut(s) 1618
MlyI GAGTC 3 cut(s) 269, 761, 1223
MmeI TCCRAC 1 cut(s) 2143
Mox20I TGGCCA 1 cut(s) 1618
MroI TCCGGA 1 cut(s) 1875
MscI TGGCCA 1 cut(s) 1618
MseI TTAA 7 cut(s) 381, 413, 743, 1268, 1670, 2022, 2201
MslI CAYNNNNRTG 1 cut(s) 723
Msp20I TGGCCA 1 cut(s) 1618
MspA1I CMGCKG 1 cut(s) 1580
MspCI CTTAAG 1 cut(s) 2021
MspI CCGG 6 cut(s) 703, 1307, 1389, 1429, 1876, 1924
MspR9I CCNGG 6 cut(s) 704, 1405, 1429, 1796, 1924, 1925
MunI CAATTG 2 cut(s) 1071, 1464
Mva1269I GAATGC 1 cut(s) 2303
MvaI CCWGG 2 cut(s) 1405, 1796
MvnI CGCG 2 cut(s) 58, 60
NciI CCSGG 4 cut(s) 704, 1429, 1924, 1925
NdeI CATATG 4 cut(s) 635, 1452, 1767, 2287
NdeII GATC 8 cut(s) 499, 545, 859, 1000, 1418, 1469, 1777, 2205
NheI GCTAGC 1 cut(s) 1286
NlaIV GGNNCC 5 cut(s) 171, 1708, 2185, 2207, 2280
NmuCI GTSAC 6 cut(s) 261, 428, 620, 1226, 1810, 2006
NspI RCATGY 5 cut(s) 1508, 1685, 1746, 1755, 2272
PaeI GCATGC 1 cut(s) 1508
PciSI GCTCTTC 1 cut(s) 1093
PctI GAATGC 1 cut(s) 2303
PfeI GAWTC 5 cut(s) 191, 776, 1488, 1539, 2236
PinAI ACCGGT 1 cut(s) 1388
PleI GAGTC 3 cut(s) 268, 761, 1223
PpsI GAGTC 3 cut(s) 268, 761, 1223
Psp124BI GAGCTC 2 cut(s) 733, 1875
Psp6I CCWGG 2 cut(s) 1403, 1794
PspEI GGTNACC 1 cut(s) 428
PspGI CCWGG 2 cut(s) 1403, 1794
PspN4I GGNNCC 5 cut(s) 171, 1708, 2185, 2207, 2280
PspPI GGNCC 2 cut(s) 21, 200
PstI CTGCAG 3 cut(s) 1162, 1573, 1585
PstNI CAGNNNCTG 1 cut(s) 1580
PsuI RGATCY 4 cut(s) 545, 1418, 1469, 2205
PvuII CAGCTG 1 cut(s) 1580
RsaI GTAC 6 cut(s) 63, 632, 681, 1426, 1438, 1689
RsaNI GTAC 6 cut(s) 62, 631, 680, 1425, 1437, 1688
RseI CAYNNNNRTG 1 cut(s) 723
SacI GAGCTC 2 cut(s) 733, 1875
SalI GTCGAC 1 cut(s) 1459
SapI GCTCTTC 1 cut(s) 1093
SaqAI TTAA 7 cut(s) 381, 413, 743, 1268, 1670, 2022, 2201
Sau3AI GATC 8 cut(s) 499, 545, 859, 1000, 1418, 1469, 1777, 2205
Sau96I GGNCC 2 cut(s) 21, 200
ScaI AGTACT 1 cut(s) 681
SchI GAGTC 3 cut(s) 269, 761, 1223
ScrFI CCNGG 6 cut(s) 704, 1405, 1429, 1796, 1924, 1925
SduI GDGCHC 4 cut(s) 33, 733, 1711, 1875
SfaNI GCATC 7 cut(s) 214, 511, 1306, 1441, 1642, 1829, 1928
SfcI CTRYAG 6 cut(s) 989, 1158, 1569, 1581, 2049, 2080
SmaI CCCGGG 1 cut(s) 1925
SmiMI CAYNNNNRTG 1 cut(s) 723
SmlI CTYRAG 4 cut(s) 421, 1889, 2021, 2273
SmoI CTYRAG 4 cut(s) 421, 1889, 2021, 2273
SphI GCATGC 1 cut(s) 1508
Sse9I AATT 7 cut(s) 34, 184, 1071, 1269, 1464, 1611, 2085
SsiI CCGC 7 cut(s) 56, 134, 293, 1303, 1502, 1725, 2250
SspI AATATT 1 cut(s) 287
SspMI CTAG 9 cut(s) 219, 228, 486, 855, 1080, 1287, 1422, 2118, 2302
SstI GAGCTC 2 cut(s) 733, 1875
StyD4I CCNGG 6 cut(s) 702, 1403, 1427, 1794, 1922, 1923
StyI CCWWGG 3 cut(s) 592, 2187, 2292
TaiI ACGT 3 cut(s) 966, 1812, 1976
TaqI TCGA 3 cut(s) 782, 1460, 1837
TasI AATT 7 cut(s) 34, 184, 1071, 1269, 1464, 1611, 2085
TatI WGTACW 3 cut(s) 630, 679, 1687
TauI GCSGC 1 cut(s) 1306
TfiI GAWTC 5 cut(s) 191, 776, 1488, 1539, 2236
Tru1I TTAA 7 cut(s) 381, 413, 743, 1268, 1670, 2022, 2201
Tru9I TTAA 7 cut(s) 381, 413, 743, 1268, 1670, 2022, 2201
TscAI CASTG 7 cut(s) 94, 357, 1020, 1167, 1603, 1949, 2058
TseFI GTSAC 6 cut(s) 261, 428, 620, 1226, 1810, 2006
Tsp45I GTSAC 6 cut(s) 261, 428, 620, 1226, 1810, 2006
TspDTI ATGAA 8 cut(s) 449, 909, 1068, 1146, 1491, 2141, 2216, 2277
TspGWI ACGGA 5 cut(s) 30, 66, 184, 381, 1309
TspMI CCCGGG 1 cut(s) 1923
TspRI CASTG 7 cut(s) 94, 357, 1020, 1167, 1603, 1949, 2058
Vha464I CTTAAG 1 cut(s) 2021
XapI RAATTY 2 cut(s) 1611, 2085
XceI RCATGY 5 cut(s) 1508, 1685, 1746, 1755, 2272
XcmI CCANNNNNNNNNTGG 1 cut(s) 909
XmaI CCCGGG 1 cut(s) 1923
XmiI GTMKAC 2 cut(s) 1460, 2079
XspI CTAG 9 cut(s) 219, 228, 486, 855, 1080, 1287, 1422, 2118, 2302
ZrmI AGTACT 1 cut(s) 681
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.