RchiOBHm_Chr4g0396141

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0001434
Physical Location & Seq
Reverse (-)
12222218 .. 12230548
8331 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36851

Sequence Viewer

Length: 2310 bp
ATGAGGGCAGGTTCGTTGTGGGCCGACGAGCCCAATCCTCCTTCTACCTCCGTGCCCCGCGTTCCCCAGCCCAATCCCTCCTCCAAGCACAGTGTTAGCAATGTGTTTAGCTTGTTAGCACGGAGAGAGGTCTCTCCTCAAACAAAACATTCGGCAAAGAAGTTATGGGGGAAAGCTTCCAAGAGTCAATCTGATAACATTGGCCCAAGGTGTGAAGCAGCTAGAGATGCTAGAAATGGCCTTGTGTCATGGGTGGAGGCAGAGTCGCTCCAGCATTTATCTGCAAAATATTGCCCGCTTGTGCCTCCTCCACGGTCAACTATTGCAGCAGCCTTCAGCCCAGATGGAAAGACACTTGCTTCCACTCATGGAGATCATACTGTAAAGATCATTGATTGCCGAACTGGAAGGTGCTTAAAGGTGTTGAGTGGTCACCGCAGGACACCTTGGGTGGTCAGGTTTCATCCTTTGCATCCAGACATACTGGCTAGTGGAAGTCTGGATCATGAAGTGAGGTTGTGGGATTCAAACACAGCAGAGTGTATAGGATCGCGTGATTTCTTACGCCCCATTGCTTCCATAGCTTTCCATGCCCAAGGCGAGCTTCTTGCTGTTGCATCAGGTCACAAGTTATACATATGGCACTACAATAGAAGAGGAGAGTCATCCTCACCAACAATAGTACTGAAGACACGACGTTCGCTTCGGGCTGTGCATTTTCATCCTCATGCTGCTCCTTTCCTTTTAACTGCTGAGGTCAATGACCTTGACTCGTCAGATTCCTCGATGACACTCGCAACTTCTCCGGGTTACTTGCGCTATCCCCCACCTACTGTGTATTTGGCAGATGCTCATTCCAGTGATCGTACTAGTTTGGCAGATGGACTACCTCTTATGTCTTTACCATTCCTGATATGGCCTTCATTTGCTAGAGATAGTGGGAGAATATCTATGCAGCGTAGTGATGTGGATACTGGTTCAAGTAGTGCACAACAGAGAGTTGATCCTTCTGCTTCTGTGCGGCTTCTAACATATTCAACTCCATCAGGGCAGTATGAACTTCTGTTGTCCCCTATTGAACCAAATAGCTCTTCTCCAGCGCCAGAAGACACGGGAAATAGTCCCTTTTTGAGTGAAATGGAAACTGAAGTTTCTCAATCTGCTATGGGTACTTTAGAGCCTATGGAAGTGCAACCAGAAAGCAGAAGTAATCATATTTTCCCTTTTGGTGACTCAACATATTGGGAACTTCCTTTCTTGCAAGGGTGGCTAATTGGTCAGACCCAAGCTAGCCAACGGAATATGCGGCTGGTAAGTGATTCTGCCCATGACAATCCATCTACAAATGGTGAGATGGACAGCCCAGCTCCTATCACTTCCTCAGTAATATCGACCGGTGTAAACCAACCTAGGGTTACTGGAAGATCTAGTTCCCGGCACCGTACTTCACGTTCTCATATGTTGTCCGCAATTGGGTCTAATGAGGGTGCTGGATTCAATAATATTGCTCATGGTGAAAGTGAGCCTCAACCTGTTGTCAGCAGAATCCAATCTGAGCTTGCTAACTCCCTTGCTGCAGCAGCGGCGGCAGAGTTACCTTGTACTGTGAAGCTCAGAGTTTGGCCACATGATGTGAAAAATCCGTGTGCTCCCCTTGATGCTGAAAGATGTTACTTAACTATTCCGCATGCCGTACTTTGTAGTGAAATGGGAGCCCATTTTTCACCTTGTGGAAGGTTTTTAGCAGCCTGTGTTGCATGTATGTTGCCTCATTTGGAAGGTGATCCTGGATTACAGGGCCAGGGCAACCATGATATCACAGGGGCAGCAACCTCCCCAACTCGTCACCCAATTTCAGCTCACCATGTCGTGTATGAGCTCCGGATATATTCCCTCGAGGAGGCAACGTTTGGCATGGTTCTTGCATCCCGGGCAATACGAGCTGCCCATTGCTTGACATCTATTCAGTTCTCTCCTACGTCAGAACACATATTACTTGCGTATGGTCGGCGTCACAGTTCACTTCTTAAGAGTGTTGTCATTGATGGAGAGACAACAGTGCCTATTTACACCATTCTGGAGGTATATAGAGTTTCTGATATGGAACTTGTGAGAATTCTCCCTAGTGCAGAAGATGAGGTCAATGTAGCTTGTTTTCATCCTTCTGTCGGAGGTGGCCTTGTTTATGGAACTAAGGAAGGGAAGTTAAGGATCCTCCAATATGATAGTTCTCATGCCGTGGGTGATACAACTTCCAGTTTTCTTGATGAAAACATGCTAGAGGTTCCGACTTATGCTTTAGAATGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04137 ko04140 map04137 map04140
Pfam Domains
Protein Families
WD40

Protein Analysis

769

Amino Acids

83.57

Weight (kDa)

6.33

Isoelectric Point (pI)

55.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 109 - 244 3.1e-20 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 109 - 214 2.7e-12 WDR3 first beta-propeller domain
WDR55 PF24796 109 - 214 3.1e-08 WDR55
Beta-prop_WDR5 PF25175 111 - 254 5.6e-20 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 111 - 218 4.2e-12 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 111 - 209 7.9e-10 WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 112 - 217 5.4e-07 WDR36/Utp21 second beta-propeller domain
WD40_Prp19 PF24814 115 - 242 1.8e-10 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 150 - 243 4.3e-06 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 1895
AccB1I GGYRCC 1 cut(s) 1437
AccII CGCG 2 cut(s) 60, 553
AccIII TCCGGA 1 cut(s) 1881
AciI CCGC 9 cut(s) 58, 296, 436, 1021, 1306, 1467, 1583, 1586, 1685
AclI AACGTT 1 cut(s) 1907
AclWI GGATC 6 cut(s) 510, 556, 998, 1778, 2206, 2219
AcoI YGGCCR 1 cut(s) 1622
AcsI RAATTY 1 cut(s) 2115
AcuI CTGAAG 3 cut(s) 319, 707, 1167
AcyI GRCGYC 1 cut(s) 2011
AdeI CACNNNGTG 1 cut(s) 1730
AfaI GTAC 6 cut(s) 684, 868, 1171, 1444, 1603, 1695
AfiI CCNNNNNNNGG 4 cut(s) 1411, 1473, 1900, 2168
AflII CTTAAG 1 cut(s) 2027
AgeI ACCGGT 1 cut(s) 1394
AgsI TTSAA 5 cut(s) 528, 981, 1038, 1079, 1498
AhlI ACTAGT 1 cut(s) 869
AjnI CCWGG 2 cut(s) 1786, 1800
AjuI GAANNNNNNNTTGG 4 cut(s) 588, 620, 1075, 1107
Alw21I GWGCWC 3 cut(s) 991, 1651, 1881
Alw26I GTCTC 2 cut(s) 136, 2045
Alw44I GTGCAC 1 cut(s) 987
AlwI GGATC 6 cut(s) 510, 556, 998, 1778, 2206, 2219
Ama87I CYCGRG 2 cut(s) 1895, 1929
Aor13HI TCCGGA 1 cut(s) 1881
AoxI GGCC 7 cut(s) 21, 202, 238, 917, 1622, 1798, 2176
ApaLI GTGCAC 1 cut(s) 987
ApoI RAATTY 1 cut(s) 2115
ArsI GACNNNNNNTTYG 4 cut(s) 682, 687, 714, 719
AsiGI ACCGGT 1 cut(s) 1394
AspA2I CCTAGG 1 cut(s) 1409
AspLEI GCGC 2 cut(s) 819, 1102
AspS9I GGNCC 3 cut(s) 21, 203, 1798
AsuC2I CCSGG 4 cut(s) 807, 1435, 1930, 1931
AsuNHI GCTAGC 1 cut(s) 1289
AvaI CYCGRG 2 cut(s) 1895, 1929
AvrII CCTAGG 1 cut(s) 1409
BaeGI GKGCMC 2 cut(s) 57, 991
BalI TGGCCA 1 cut(s) 1624
BamHI GGATCC 1 cut(s) 2211
BanI GGYRCC 1 cut(s) 1437
BanII GRGCYC 3 cut(s) 33, 1717, 1881
BbsI GAAGAC 2 cut(s) 695, 1113
Bbv12I GWGCWC 3 cut(s) 991, 1651, 1881
BbvCI CCTCAGC 1 cut(s) 753
BccI CCATC 6 cut(s) 338, 875, 1051, 1345, 1348, 2039
BceAI ACGGC 2 cut(s) 1676, 2222
BcgI CGANNNNNNTGC 2 cut(s) 590, 624
BciT130I CCWGG 2 cut(s) 1788, 1802
BciVI GTATCC 1 cut(s) 964
BcnI CCSGG 4 cut(s) 807, 1435, 1930, 1931
BcoDI GTCTC 2 cut(s) 136, 2045
BcuI ACTAGT 1 cut(s) 869
BfmI CTRYAG 1 cut(s) 1575
BfoI RGCGCY 1 cut(s) 1103
BfrI CTTAAG 1 cut(s) 2027
BfuI GTATCC 1 cut(s) 964
BglII AGATCT 1 cut(s) 1424
BlnI CCTAGG 1 cut(s) 1409
BmcAI AGTACT 1 cut(s) 684
Bme1390I CCNGG 6 cut(s) 807, 1435, 1788, 1802, 1930, 1931
BmeT110I CYCGRG 2 cut(s) 1895, 1929
BmgT120I GGNCC 3 cut(s) 21, 203, 1798
BmiI GGNNCC 4 cut(s) 1439, 1714, 2213, 2286
BmrFI CCNGG 6 cut(s) 807, 1435, 1788, 1802, 1930, 1931
BmsI GCATC 6 cut(s) 217, 481, 626, 838, 1648, 1934
BmtI GCTAGC 1 cut(s) 1293
BpiI GAAGAC 2 cut(s) 695, 1113
BplI GAGNNNNNCTC 2 cut(s) 653, 685
BpmI CTGGAG 3 cut(s) 254, 1080, 2099
Bpu10I CCTNAGC 1 cut(s) 753
BpuMI CCSGG 4 cut(s) 807, 1435, 1930, 1931
BsaHI GRCGYC 1 cut(s) 2011
BsaI GGTCTC 1 cut(s) 136
BsaJI CCNNGG 8 cut(s) 206, 311, 446, 595, 1409, 1801, 1929, 2238
BsaWI WCCGGW 2 cut(s) 1394, 1881
BsaXI ACNNNNNCTCC 4 cut(s) 248, 278, 1892, 1922
Bsc4I CCNNNNNNNGG 4 cut(s) 1411, 1473, 1900, 2168
Bse118I RCCGGY 1 cut(s) 1394
Bse1I ACTGG 6 cut(s) 409, 489, 858, 979, 1423, 2256
Bse3DI GCAATG 3 cut(s) 106, 570, 1948
BseAI TCCGGA 1 cut(s) 1881
BseBI CCWGG 2 cut(s) 1788, 1802
BseDI CCNNGG 8 cut(s) 206, 311, 446, 595, 1409, 1801, 1929, 2238
BseGI GGATG 6 cut(s) 463, 472, 665, 721, 1925, 2158
BseLI CCNNNNNNNGG 4 cut(s) 1411, 1473, 1900, 2168
BseMI GCAATG 3 cut(s) 106, 570, 1948
BseMII CTCAG 4 cut(s) 744, 1395, 1545, 1627
BseNI ACTGG 6 cut(s) 409, 489, 858, 979, 1423, 2256
BseRI GAGGAG 5 cut(s) 70, 126, 297, 672, 1913
BseSI GKGCMC 2 cut(s) 57, 991
BseYI CCCAGC 2 cut(s) 66, 1363
BsgI GTGCAG 1 cut(s) 2148
Bsh1236I CGCG 2 cut(s) 60, 553
Bsh1285I CGRYCG 1 cut(s) 1395
BshFI GGCC 7 cut(s) 23, 204, 240, 919, 1624, 1800, 2178
BshNI GGYRCC 1 cut(s) 1437
BshTI ACCGGT 1 cut(s) 1394
BsiEI CGRYCG 1 cut(s) 1395
BsiHKAI GWGCWC 3 cut(s) 991, 1651, 1881
BsiHKCI CYCGRG 2 cut(s) 1895, 1929
BsiSI CCGG 5 cut(s) 806, 1395, 1435, 1882, 1930
BslFI GGGAC 2 cut(s) 1054, 1107
BslI CCNNNNNNNGG 4 cut(s) 1411, 1473, 1900, 2168
BsmAI GTCTC 2 cut(s) 136, 2045
BsmFI GGGAC 2 cut(s) 1054, 1107
BsmI GAATGC 1 cut(s) 2309
BsnI GGCC 7 cut(s) 23, 204, 240, 919, 1624, 1800, 2178
Bso31I GGTCTC 1 cut(s) 136
BsoBI CYCGRG 2 cut(s) 1895, 1929
Bsp1286I GDGCHC 6 cut(s) 33, 57, 991, 1651, 1717, 1881
Bsp13I TCCGGA 1 cut(s) 1881
Bsp143I GATC 9 cut(s) 373, 387, 502, 548, 862, 1003, 1424, 1783, 2211
BspACI CCGC 9 cut(s) 58, 296, 436, 1021, 1306, 1467, 1583, 1586, 1685
BspANI GGCC 7 cut(s) 23, 204, 240, 919, 1624, 1800, 2178
BspCNI CTCAG 4 cut(s) 745, 1394, 1546, 1626
BspEI TCCGGA 1 cut(s) 1881
BspFNI CGCG 2 cut(s) 60, 553
BspHI TCATGA 1 cut(s) 505
BspLI GGNNCC 4 cut(s) 1439, 1714, 2213, 2286
BspMAI CTGCAG 1 cut(s) 1579
BspOI GCTAGC 1 cut(s) 1293
BspPI GGATC 6 cut(s) 510, 556, 998, 1778, 2206, 2219
BspQI GCTCTTC 1 cut(s) 1096
BspT107I GGYRCC 1 cut(s) 1437
BspTI CTTAAG 1 cut(s) 2027
BspTNI GGTCTC 1 cut(s) 136
BsrDI GCAATG 3 cut(s) 106, 570, 1948
BsrFI RCCGGY 1 cut(s) 1394
BsrI ACTGG 6 cut(s) 409, 489, 858, 979, 1423, 2256
BssAI RCCGGY 1 cut(s) 1394
BssECI CCNNGG 8 cut(s) 206, 311, 446, 595, 1409, 1801, 1929, 2238
BssMI GATC 9 cut(s) 373, 387, 502, 548, 862, 1003, 1424, 1783, 2211
BssNI GRCGYC 1 cut(s) 2011
BssT1I CCWWGG 4 cut(s) 206, 446, 595, 1409
Bst2UI CCWGG 2 cut(s) 1788, 1802
Bst4CI ACNGT 8 cut(s) 92, 315, 382, 835, 1442, 1606, 2018, 2059
Bst6I CTCTTC 2 cut(s) 649, 1096
BstACI GRCGYC 1 cut(s) 2011
BstAFI CTTAAG 1 cut(s) 2027
BstC8I GCNNGC 5 cut(s) 296, 602, 1291, 1560, 1689
BstDEI CTNAG 5 cut(s) 753, 1381, 1554, 1613, 2193
BstDSI CCRYGG 2 cut(s) 311, 2238
BstEII GGTNACC 1 cut(s) 431
BstENI CCTNNNNNAGG 1 cut(s) 1898
BstF5I GGATG 6 cut(s) 463, 472, 665, 721, 1925, 2158
BstFNI CGCG 2 cut(s) 60, 553
BstH2I RGCGCY 1 cut(s) 1103
BstHHI GCGC 2 cut(s) 819, 1102
BstKTI GATC 9 cut(s) 376, 390, 505, 551, 865, 1006, 1427, 1786, 2214
BstMAI GTCTC 2 cut(s) 136, 2045
BstMBI GATC 9 cut(s) 373, 387, 502, 548, 862, 1003, 1424, 1783, 2211
BstMCI CGRYCG 1 cut(s) 1395
BstNI CCWGG 2 cut(s) 1788, 1802
BstNSI RCATGY 3 cut(s) 1691, 1761, 2278
BstPI GGTNACC 1 cut(s) 431
BstSCI CCNGG 6 cut(s) 805, 1433, 1786, 1800, 1928, 1929
BstSFI CTRYAG 1 cut(s) 1575
BstSLI GKGCMC 2 cut(s) 57, 991
BstUI CGCG 2 cut(s) 60, 553
BstV2I GAAGAC 2 cut(s) 695, 1113
BstX2I RGATCY 2 cut(s) 1424, 2211
BstYI RGATCY 2 cut(s) 1424, 2211
BsuI GTATCC 1 cut(s) 964
BsuRI GGCC 7 cut(s) 23, 204, 240, 919, 1624, 1800, 2178
BtgI CCRYGG 2 cut(s) 311, 2238
BtsCI GGATG 6 cut(s) 463, 472, 665, 721, 1925, 2158
BtsIMutI CAGTG 3 cut(s) 97, 865, 2064
Cac8I GCNNGC 5 cut(s) 296, 602, 1291, 1560, 1689
CciI TCATGA 1 cut(s) 505
CfoI GCGC 2 cut(s) 819, 1102
Cfr10I RCCGGY 1 cut(s) 1394
Cfr13I GGNCC 3 cut(s) 21, 203, 1798
Cfr9I CCCGGG 1 cut(s) 1929
CseI GACGC 1 cut(s) 2000
Csp6I GTAC 6 cut(s) 683, 867, 1170, 1443, 1602, 1694
CspAI ACCGGT 1 cut(s) 1394
CviQI GTAC 6 cut(s) 683, 867, 1170, 1443, 1602, 1694
DdeI CTNAG 5 cut(s) 753, 1381, 1554, 1613, 2193
DpnI GATC 9 cut(s) 375, 389, 504, 550, 864, 1005, 1426, 1785, 2213
DpnII GATC 9 cut(s) 373, 387, 502, 548, 862, 1003, 1424, 1783, 2211
DraIII CACNNNGTG 1 cut(s) 1730
EaeI YGGCCR 1 cut(s) 1622
Eam1104I CTCTTC 2 cut(s) 649, 1096
EarI CTCTTC 2 cut(s) 649, 1096
Ecl136II GAGCTC 1 cut(s) 1879
Eco130I CCWWGG 4 cut(s) 206, 446, 595, 1409
Eco24I GRGCYC 3 cut(s) 33, 1717, 1881
Eco31I GGTCTC 1 cut(s) 136
Eco32I GATATC 1 cut(s) 1816
Eco53kI GAGCTC 1 cut(s) 1879
Eco57I CTGAAG 3 cut(s) 319, 707, 1167
Eco88I CYCGRG 2 cut(s) 1895, 1929
Eco91I GGTNACC 1 cut(s) 431
EcoICRI GAGCTC 1 cut(s) 1879
EcoNI CCTNNNNNAGG 1 cut(s) 1898
EcoO65I GGTNACC 1 cut(s) 431
EcoRI GAATTC 1 cut(s) 2115
EcoRII CCWGG 2 cut(s) 1786, 1800
EcoRV GATATC 1 cut(s) 1816
EcoT14I CCWWGG 4 cut(s) 206, 446, 595, 1409
EcoT38I GRGCYC 3 cut(s) 33, 1717, 1881
ErhI CCWWGG 4 cut(s) 206, 446, 595, 1409
FalI AAGNNNNNCTT 2 cut(s) 588, 620
FaqI GGGAC 2 cut(s) 1054, 1107
FauI CCCGC 2 cut(s) 65, 303
FauNDI CATATG 2 cut(s) 638, 1458
FokI GGATG 6 cut(s) 450, 459, 652, 708, 1912, 2145
FriOI GRGCYC 3 cut(s) 33, 1717, 1881
GlaI GCGC 2 cut(s) 818, 1101
GsaI CCCAGC 2 cut(s) 70, 1367
GsuI CTGGAG 3 cut(s) 254, 1080, 2099
HaeII RGCGCY 1 cut(s) 1103
HaeIII GGCC 7 cut(s) 23, 204, 240, 919, 1624, 1800, 2178
HapII CCGG 5 cut(s) 806, 1395, 1435, 1882, 1930
HgaI GACGC 1 cut(s) 2000
HhaI GCGC 2 cut(s) 819, 1102
Hin1I GRCGYC 1 cut(s) 2011
Hin6I GCGC 2 cut(s) 817, 1100
HinP1I GCGC 2 cut(s) 817, 1100
HincII GTYRAC 1 cut(s) 318
HindII GTYRAC 1 cut(s) 318
HindIII AAGCTT 1 cut(s) 174
HpaII CCGG 5 cut(s) 806, 1395, 1435, 1882, 1930
Hpy166II GTNNAC 4 cut(s) 318, 989, 1402, 2021
Hpy188I TCNGA 9 cut(s) 193, 778, 1281, 1555, 1616, 1984, 2098, 2171, 2289
Hpy188III TCNNGA 7 cut(s) 476, 500, 506, 910, 1882, 2078, 2264
Hpy8I GTNNAC 4 cut(s) 318, 989, 1402, 2021
Hpy99I CGWCG 2 cut(s) 29, 699
HpyAV CCTTC 9 cut(s) 51, 343, 402, 930, 1017, 1728, 1772, 2172, 2192
HpyCH4III ACNGT 8 cut(s) 92, 315, 382, 835, 1442, 1606, 2018, 2059
HpyCH4IV ACGT 4 cut(s) 697, 1450, 1907, 1979
HpyF3I CTNAG 5 cut(s) 753, 1381, 1554, 1613, 2193
HpySE526I ACGT 4 cut(s) 697, 1450, 1907, 1979
Hsp92I GRCGYC 1 cut(s) 2011
HspAI GCGC 2 cut(s) 817, 1100
Kpn2I TCCGGA 1 cut(s) 1881
Kzo9I GATC 9 cut(s) 373, 387, 502, 548, 862, 1003, 1424, 1783, 2211
LguI GCTCTTC 1 cut(s) 1096
LmnI GCTCC 6 cut(s) 273, 739, 1372, 1654, 1712, 1884
LweI GCATC 6 cut(s) 217, 481, 626, 838, 1648, 1934
MaeII ACGT 4 cut(s) 697, 1450, 1907, 1979
MaeIII GTNAC 9 cut(s) 431, 623, 809, 1229, 1414, 1593, 1670, 1844, 2012
MalI GATC 9 cut(s) 375, 389, 504, 550, 864, 1005, 1426, 1785, 2213
MboI GATC 9 cut(s) 373, 387, 502, 548, 862, 1003, 1424, 1783, 2211
MboII GAAGA 6 cut(s) 666, 700, 1083, 1118, 1434, 2144
MfeI CAATTG 1 cut(s) 1470
MflI RGATCY 2 cut(s) 1424, 2211
MhlI GDGCHC 6 cut(s) 33, 57, 991, 1651, 1717, 1881
MlsI TGGCCA 1 cut(s) 1624
MluCI AATT 4 cut(s) 1272, 1470, 1851, 2115
MluNI TGGCCA 1 cut(s) 1624
MlyI GAGTC 5 cut(s) 193, 272, 671, 764, 1226
MmeI TCCRAC 1 cut(s) 2149
Mox20I TGGCCA 1 cut(s) 1624
MroI TCCGGA 1 cut(s) 1881
MscI TGGCCA 1 cut(s) 1624
MseI TTAA 5 cut(s) 416, 746, 1676, 2028, 2207
MslI CAYNNNNRTG 2 cut(s) 726, 858
Msp20I TGGCCA 1 cut(s) 1624
MspA1I CMGCKG 1 cut(s) 1583
MspCI CTTAAG 1 cut(s) 2027
MspI CCGG 5 cut(s) 806, 1395, 1435, 1882, 1930
MspR9I CCNGG 6 cut(s) 807, 1435, 1788, 1802, 1930, 1931
MunI CAATTG 1 cut(s) 1470
Mva1269I GAATGC 1 cut(s) 2309
MvaI CCWGG 2 cut(s) 1788, 1802
MvnI CGCG 2 cut(s) 60, 553
NciI CCSGG 4 cut(s) 807, 1435, 1930, 1931
NdeI CATATG 2 cut(s) 638, 1458
NdeII GATC 9 cut(s) 373, 387, 502, 548, 862, 1003, 1424, 1783, 2211
NheI GCTAGC 1 cut(s) 1289
NlaIV GGNNCC 4 cut(s) 1439, 1714, 2213, 2286
NmuCI GTSAC 5 cut(s) 431, 623, 1229, 1844, 2012
NspI RCATGY 3 cut(s) 1691, 1761, 2278
PaeI GCATGC 1 cut(s) 1691
PaeR7I CTCGAG 1 cut(s) 1895
PagI TCATGA 1 cut(s) 505
PciSI GCTCTTC 1 cut(s) 1096
PctI GAATGC 1 cut(s) 2309
PfeI GAWTC 5 cut(s) 524, 779, 1319, 1494, 1545
PfoI TCCNGGA 1 cut(s) 1786
PinAI ACCGGT 1 cut(s) 1394
PleI GAGTC 5 cut(s) 192, 271, 670, 764, 1226
PpsI GAGTC 5 cut(s) 192, 271, 670, 764, 1226
Psp124BI GAGCTC 1 cut(s) 1881
Psp1406I AACGTT 1 cut(s) 1907
Psp6I CCWGG 2 cut(s) 1786, 1800
PspEI GGTNACC 1 cut(s) 431
PspFI CCCAGC 2 cut(s) 66, 1363
PspGI CCWGG 2 cut(s) 1786, 1800
PspN4I GGNNCC 4 cut(s) 1439, 1714, 2213, 2286
PspPI GGNCC 3 cut(s) 21, 203, 1798
PspXI VCTCGAGB 1 cut(s) 1895
PsrI GAACNNNNNNTAC 4 cut(s) 1435, 1467, 1977, 2009
PstI CTGCAG 1 cut(s) 1579
PsuI RGATCY 2 cut(s) 1424, 2211
RsaI GTAC 6 cut(s) 684, 868, 1171, 1444, 1603, 1695
RsaNI GTAC 6 cut(s) 683, 867, 1170, 1443, 1602, 1694
RseI CAYNNNNRTG 2 cut(s) 726, 858
SacI GAGCTC 1 cut(s) 1881
SapI GCTCTTC 1 cut(s) 1096
SaqAI TTAA 5 cut(s) 416, 746, 1676, 2028, 2207
Sau3AI GATC 9 cut(s) 373, 387, 502, 548, 862, 1003, 1424, 1783, 2211
Sau96I GGNCC 3 cut(s) 21, 203, 1798
ScaI AGTACT 1 cut(s) 684
SchI GAGTC 5 cut(s) 193, 272, 671, 764, 1226
ScrFI CCNGG 6 cut(s) 807, 1435, 1788, 1802, 1930, 1931
SduI GDGCHC 6 cut(s) 33, 57, 991, 1651, 1717, 1881
SfaNI GCATC 6 cut(s) 217, 481, 626, 838, 1648, 1934
SfcI CTRYAG 1 cut(s) 1575
Sfr274I CTCGAG 1 cut(s) 1895
SlaI CTCGAG 1 cut(s) 1895
SmaI CCCGGG 1 cut(s) 1931
SmiMI CAYNNNNRTG 2 cut(s) 726, 858
SmlI CTYRAG 2 cut(s) 1895, 2027
SmoI CTYRAG 2 cut(s) 1895, 2027
SpeI ACTAGT 1 cut(s) 869
SphI GCATGC 1 cut(s) 1691
Sse9I AATT 4 cut(s) 1272, 1470, 1851, 2115
SsiI CCGC 9 cut(s) 58, 296, 436, 1021, 1306, 1467, 1583, 1586, 1685
SspI AATATT 2 cut(s) 290, 1504
SstI GAGCTC 1 cut(s) 1881
StyD4I CCNGG 6 cut(s) 805, 1433, 1786, 1800, 1928, 1929
StyI CCWWGG 4 cut(s) 206, 446, 595, 1409
TaaI ACNGT 8 cut(s) 92, 315, 382, 835, 1442, 1606, 2018, 2059
TaiI ACGT 4 cut(s) 700, 1453, 1910, 1982
TaqI TCGA 3 cut(s) 785, 1391, 1896
TasI AATT 4 cut(s) 1272, 1470, 1851, 2115
TatI WGTACW 2 cut(s) 682, 1601
TauI GCSGC 4 cut(s) 1024, 1309, 1586, 1589
TfiI GAWTC 5 cut(s) 524, 779, 1319, 1494, 1545
Tru1I TTAA 5 cut(s) 416, 746, 1676, 2028, 2207
Tru9I TTAA 5 cut(s) 416, 746, 1676, 2028, 2207
TscAI CASTG 3 cut(s) 97, 865, 2064
TseFI GTSAC 5 cut(s) 431, 623, 1229, 1844, 2012
Tsp45I GTSAC 5 cut(s) 431, 623, 1229, 1844, 2012
TspDTI ATGAA 7 cut(s) 452, 522, 710, 912, 1071, 2147, 2283
TspGWI ACGGA 4 cut(s) 40, 136, 1312, 1632
TspMI CCCGGG 1 cut(s) 1929
TspRI CASTG 3 cut(s) 97, 865, 2064
Vha464I CTTAAG 1 cut(s) 2027
VneI GTGCAC 1 cut(s) 987
XagI CCTNNNNNAGG 1 cut(s) 1898
XapI RAATTY 1 cut(s) 2115
XceI RCATGY 3 cut(s) 1691, 1761, 2278
XcmI CCANNNNNNNNNTGG 1 cut(s) 912
XhoI CTCGAG 1 cut(s) 1895
XmaI CCCGGG 1 cut(s) 1929
XmaJI CCTAGG 1 cut(s) 1409
ZrmI AGTACT 1 cut(s) 684
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.