Rh4BG065000

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0001434
Physical Location & Seq
Reverse (-)
11624605 .. 11633122
8518 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG065000.1

Sequence Viewer

Length: 2319 bp
ATGAGGGCAGGTTCGTTGTGGGCCGACGAGCCCAATCCTCCTTCTACCTCCGTGCCCCGCGTTCCCCAGCCCAATCCCTCCAAGCACAGTGTTAGCAATGTGTTTAGCTTGTTAGCACGGAGAGAGCTCTCTCCTCAAACAAAACATTCCCCGAAGAAGTTATGGGGGAAAGCTTCCAAGAGTCAGTCTGATAACATTGGCCCAAGGTGTGAAGCAGCTAGAGATGCTAGAAATGGCCTTGTGTCATGGGTGGAGGCAGAGTCGCTCCAGCATTTATCTGCAAAATATTGCCCGCTTGTGCCTCCTCCACGGTCAACTATTGCAGCAGCCTTCAGCCCTGATGGAAAGACACTTGCTTCCACTCATGGAGATCATACTGTAAAGATCATTGATTGCCGAACTGGAAGATGCTTAAAGGTGTTGAGTGGTCACCGCAGGACACCTTGGGTGGTCAGGTTTCATCCTTTGCATCCAGACATACTGGCTAGTGGAAGTCTGGATCATGAAGTGAGGTTGTGGGATTCAAACACGGCAGAGTGTATAGGATCGCGTGATTTCTTACGCCCCATTGCTTCCATAGCTTTCCATGCCCAAGGCGAGCTTCTTGCTGTTGCATCAGGTCACAAGTTATACATATGGCACTACAATAGAAGAGGAGAGTCATCCTCACCAACAATAGTACTGAAGACACGACGTTCGCTTCGGGCTGTGCATTTTCATCCTCATGCTGCTCCTTTCCTTTTAACTGCTGAGGTCAATGACCTTGACTCGTCAGATTCCTCGATGACACTCGCAACTTCTCCGGGTTACTTGCGCTATCCCCCACCTACTGTGTATTTGGCAGATGCTCATTCCAGTGATCGTACTAGTTTGGCAGATGGACTACCTCTTATGTCTTTACCATTCCTGATATGGCCTTCATTTGCTAGAGATAGTGGGAGAATATCTATGCAGCGTAGTGATGTGGATATTGGTTCAAGTAGTGCACAACAGAGAGTTGATCCTTCTGCTTCTGTGCGGCTCCTAACATATTCAACTCCATCAGGGCAGTATGAACTTCTGATGTCCGCTATTGAACCAAATAGCCCTTCTCCAGCTCCAGAAGACACGGGAAATAGTCCGTTTTTGAGTGAAATGGAAACTGAAGTTTCTCAATCTGCTATGGGTACTTTAGAGCCTATGGAAGTGCAACCAGAAAGCAGAAGTAATCATATTTTCCCTTTTGGTGACTCAACATATTGGGAACTTCCTTTCTTGCAAGGGTGGCTAATTGGTCAGACCCAAGCCAGCCAAAGGAATATGCGGCTGGTAAGTGATTCTGCCCATGACAATCCATCTACAAATGGTGAGATGGACAGCCCAGCTCCTATCTCTTCCTCAGTAATACCGACCGGTGTAAACCAACCTAGGGTTCCTGGAAGATCTAGTTCCCGGCACCGTACTTCACGCTCTCATGTGTTGTCAGCAATTGGGTCTAATGAGGGTGCTGGATTCAATAATATTGCTCATGGTGAAAGTGAGTCTCAACCTGTTAGCAGAATCCAATCTGAGCTTGCTAACTCCCTTGCTGCAGCAGCGGCAGCAGAGTTACCTTGTACCGTGAAGCTCAGAGTTTGGCCACATGATGTGAAAAATCCGTGTGCTCCCCTTGATGCTGGGAGATGTTTCTTAACCATTCCGCATGCCGTACTTTGTAGTGAAATGGGAGCCCATTTTTCACCTTGCGGAAGGTTTTTAGCAGCCTGTGTTGCATGTATGTTGCCTCATTTGGAAGGTGATCCTGGATTACAGGGCCAGGCCAACCATGATATCACAGGGGCAGCAACCTCCCCAACTCGTCACCCAATTTCAGCTCACCATGTCGTGTATGAGCTCCGGATATATTCCCTCGAGCAGGCAACGTTTGGCATGGTTCTTACATCCCGGGCAATACGAGCTGCCCATTGCTTGACATCTATTCAGTTCTCTCCTACGTCAGAACACATATTACTTGCGTATGGCCGGCGTCACAGTTCACTTCTTAAGAGTGTTGTCATTGATGGAGAGACAACAGTGCCTATTTACACCATTCTGGAGGTATATAGAGTTTCTGATATGGAACTTGTGAGAATTCTCCCTAGTGCAGAAGATGAGGTCAACGTAGCTTGTTTTCATCCTTCTGTTGGAGGTGGCCTTGTTTATGGAACTAAGGAAGGAAAGTTAAGGATCCTCCAATATGATAGTTCTCATGCCGTGGGTCATACAACTTCCAGTTTTCTTGATGAAAACATGTTAGAGGTACGACAAGGCCCCTGTAGTTCATTTGTTTGTTTCTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04137 ko04140 map04137 map04140
Pfam Domains
Protein Families
WD40

Protein Analysis

772

Amino Acids

83.91

Weight (kDa)

6.66

Isoelectric Point (pI)

55.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 108 - 243 3.1e-20 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 108 - 213 2.7e-12 WDR3 first beta-propeller domain
WDR55 PF24796 108 - 213 3.1e-08 WDR55
Beta-prop_WDR5 PF25175 110 - 253 5.7e-20 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 110 - 217 4.2e-12 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 110 - 209 4.7e-10 WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 111 - 216 5.5e-07 WDR36/Utp21 second beta-propeller domain
WD40_Prp19 PF24814 114 - 241 1.8e-10 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 149 - 242 4.3e-06 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1434
AccII CGCG 2 cut(s) 60, 550
AccIII TCCGGA 1 cut(s) 1875
AciI CCGC 9 cut(s) 58, 293, 433, 1018, 1068, 1303, 1577, 1679, 1725
AclI AACGTT 1 cut(s) 1901
AclWI GGATC 6 cut(s) 507, 553, 995, 1772, 2200, 2213
AcoI YGGCCR 2 cut(s) 1616, 1999
AcsI RAATTY 1 cut(s) 2109
AcuI CTGAAG 3 cut(s) 316, 704, 1164
AcyI GRCGYC 1 cut(s) 2005
AfaI GTAC 7 cut(s) 681, 865, 1168, 1441, 1597, 1689, 2280
AfiI CCNNNNNNNGG 4 cut(s) 1293, 1408, 1894, 2162
AflII CTTAAG 1 cut(s) 2021
AflIII ACRYGT 1 cut(s) 2268
AgeI ACCGGT 1 cut(s) 1391
AgsI TTSAA 5 cut(s) 525, 978, 1035, 1076, 1495
AhlI ACTAGT 1 cut(s) 866
AjnI CCWGG 3 cut(s) 1414, 1780, 1794
AjuI GAANNNNNNNTTGG 6 cut(s) 585, 617, 1072, 1104, 1395, 1427
Alw21I GWGCWC 4 cut(s) 129, 988, 1645, 1875
Alw26I GTCTC 2 cut(s) 1527, 2039
Alw44I GTGCAC 1 cut(s) 984
AlwI GGATC 6 cut(s) 507, 553, 995, 1772, 2200, 2213
Ama87I CYCGRG 2 cut(s) 1889, 1923
Aor13HI TCCGGA 1 cut(s) 1875
ApaLI GTGCAC 1 cut(s) 984
ApoI RAATTY 1 cut(s) 2109
ArsI GACNNNNNNTTYG 4 cut(s) 679, 684, 711, 716
AsiGI ACCGGT 1 cut(s) 1391
AspA2I CCTAGG 1 cut(s) 1406
AspLEI GCGC 1 cut(s) 816
AspS9I GGNCC 4 cut(s) 21, 200, 1792, 2288
AsuC2I CCSGG 4 cut(s) 804, 1432, 1924, 1925
AsuHPI GGTGA 9 cut(s) 422, 660, 1238, 1358, 1523, 1710, 1787, 1832, 1847
AvaI CYCGRG 2 cut(s) 1889, 1923
AvrII CCTAGG 1 cut(s) 1406
BaeGI GKGCMC 2 cut(s) 57, 988
BalI TGGCCA 1 cut(s) 1618
BamHI GGATCC 1 cut(s) 2205
BanI GGYRCC 1 cut(s) 1434
BanII GRGCYC 4 cut(s) 33, 129, 1711, 1875
BbsI GAAGAC 2 cut(s) 692, 1110
Bbv12I GWGCWC 4 cut(s) 129, 988, 1645, 1875
BbvCI CCTCAGC 1 cut(s) 750
BccI CCATC 6 cut(s) 335, 872, 1048, 1342, 1345, 2033
BceAI ACGGC 3 cut(s) 546, 1670, 2216
BcgI CGANNNNNNTGC 2 cut(s) 587, 621
BciT130I CCWGG 3 cut(s) 1416, 1782, 1796
BcnI CCSGG 4 cut(s) 804, 1432, 1924, 1925
BcoDI GTCTC 2 cut(s) 1527, 2039
BcuI ACTAGT 1 cut(s) 866
BfaI CTAG 8 cut(s) 219, 228, 486, 867, 927, 1407, 1425, 2118
BfmI CTRYAG 2 cut(s) 1569, 2293
BfrI CTTAAG 1 cut(s) 2021
BglII AGATCT 1 cut(s) 1421
BlnI CCTAGG 1 cut(s) 1406
BmcAI AGTACT 1 cut(s) 681
Bme1390I CCNGG 7 cut(s) 804, 1416, 1432, 1782, 1796, 1924, 1925
BmeT110I CYCGRG 2 cut(s) 1889, 1923
BmgT120I GGNCC 4 cut(s) 21, 200, 1792, 2288
BmiI GGNNCC 6 cut(s) 1022, 1413, 1436, 1708, 2207, 2290
BmrFI CCNGG 7 cut(s) 804, 1416, 1432, 1782, 1796, 1924, 1925
BmsI GCATC 6 cut(s) 214, 398, 478, 623, 835, 1642
BpiI GAAGAC 2 cut(s) 692, 1110
BplI GAGNNNNNCTC 2 cut(s) 650, 682
BpmI CTGGAG 4 cut(s) 251, 1077, 1083, 2093
Bpu10I CCTNAGC 1 cut(s) 750
BpuMI CCSGG 4 cut(s) 804, 1432, 1924, 1925
BsaHI GRCGYC 1 cut(s) 2005
BsaJI CCNNGG 7 cut(s) 203, 308, 443, 592, 1406, 1923, 2232
BsaWI WCCGGW 2 cut(s) 1391, 1875
BsaXI ACNNNNNCTCC 2 cut(s) 245, 275
Bsc4I CCNNNNNNNGG 4 cut(s) 1293, 1408, 1894, 2162
Bse118I RCCGGY 2 cut(s) 1391, 2001
Bse1I ACTGG 4 cut(s) 406, 486, 855, 2250
Bse3DI GCAATG 3 cut(s) 103, 567, 1942
BseAI TCCGGA 1 cut(s) 1875
BseBI CCWGG 3 cut(s) 1416, 1782, 1796
BseDI CCNNGG 7 cut(s) 203, 308, 443, 592, 1406, 1923, 2232
BseGI GGATG 6 cut(s) 460, 469, 662, 718, 1919, 2152
BseLI CCNNNNNNNGG 4 cut(s) 1293, 1408, 1894, 2162
BseMI GCAATG 3 cut(s) 103, 567, 1942
BseMII CTCAG 4 cut(s) 741, 1392, 1539, 1621
BseNI ACTGG 4 cut(s) 406, 486, 855, 2250
BseRI GAGGAG 3 cut(s) 123, 294, 669
BseSI GKGCMC 2 cut(s) 57, 988
BseYI CCCAGC 3 cut(s) 66, 1360, 1655
BsgI GTGCAG 1 cut(s) 2142
Bsh1236I CGCG 2 cut(s) 60, 550
Bsh1285I CGRYCG 1 cut(s) 1392
BshNI GGYRCC 1 cut(s) 1434
BshTI ACCGGT 1 cut(s) 1391
BsiEI CGRYCG 1 cut(s) 1392
BsiHKAI GWGCWC 4 cut(s) 129, 988, 1645, 1875
BsiHKCI CYCGRG 2 cut(s) 1889, 1923
BsiSI CCGG 6 cut(s) 803, 1392, 1432, 1876, 1924, 2002
BslI CCNNNNNNNGG 4 cut(s) 1293, 1408, 1894, 2162
BsmAI GTCTC 2 cut(s) 1527, 2039
BsoBI CYCGRG 2 cut(s) 1889, 1923
Bsp1286I GDGCHC 7 cut(s) 33, 57, 129, 988, 1645, 1711, 1875
Bsp13I TCCGGA 1 cut(s) 1875
Bsp143I GATC 9 cut(s) 370, 384, 499, 545, 859, 1000, 1421, 1777, 2205
BspACI CCGC 9 cut(s) 58, 293, 433, 1018, 1068, 1303, 1577, 1679, 1725
BspCNI CTCAG 4 cut(s) 742, 1391, 1540, 1620
BspEI TCCGGA 1 cut(s) 1875
BspFNI CGCG 2 cut(s) 60, 550
BspHI TCATGA 1 cut(s) 502
BspLI GGNNCC 6 cut(s) 1022, 1413, 1436, 1708, 2207, 2290
BspMAI CTGCAG 1 cut(s) 1573
BspPI GGATC 6 cut(s) 507, 553, 995, 1772, 2200, 2213
BspT107I GGYRCC 1 cut(s) 1434
BspTI CTTAAG 1 cut(s) 2021
BsrDI GCAATG 3 cut(s) 103, 567, 1942
BsrFI RCCGGY 2 cut(s) 1391, 2001
BsrI ACTGG 4 cut(s) 406, 486, 855, 2250
BssAI RCCGGY 2 cut(s) 1391, 2001
BssECI CCNNGG 7 cut(s) 203, 308, 443, 592, 1406, 1923, 2232
BssMI GATC 9 cut(s) 370, 384, 499, 545, 859, 1000, 1421, 1777, 2205
BssNI GRCGYC 1 cut(s) 2005
BssT1I CCWWGG 4 cut(s) 203, 443, 592, 1406
Bst2UI CCWGG 3 cut(s) 1416, 1782, 1796
Bst4CI ACNGT 8 cut(s) 89, 312, 379, 832, 1439, 1600, 2012, 2053
Bst6I CTCTTC 2 cut(s) 646, 1378
BstACI GRCGYC 1 cut(s) 2005
BstAFI CTTAAG 1 cut(s) 2021
BstC8I GCNNGC 7 cut(s) 293, 599, 1288, 1554, 1683, 1896, 2003
BstDEI CTNAG 5 cut(s) 750, 1378, 1548, 1607, 2187
BstDSI CCRYGG 2 cut(s) 308, 2232
BstEII GGTNACC 1 cut(s) 428
BstENI CCTNNNNNAGG 1 cut(s) 1892
BstF5I GGATG 6 cut(s) 460, 469, 662, 718, 1919, 2152
BstFNI CGCG 2 cut(s) 60, 550
BstHHI GCGC 1 cut(s) 816
BstKTI GATC 9 cut(s) 373, 387, 502, 548, 862, 1003, 1424, 1780, 2208
BstMAI GTCTC 2 cut(s) 1527, 2039
BstMBI GATC 9 cut(s) 370, 384, 499, 545, 859, 1000, 1421, 1777, 2205
BstMCI CGRYCG 1 cut(s) 1392
BstMWI GCNNNNNNNGC 9 cut(s) 224, 578, 587, 1264, 1574, 1577, 1580, 1748, 1934
BstNI CCWGG 3 cut(s) 1416, 1782, 1796
BstNSI RCATGY 3 cut(s) 1685, 1755, 2272
BstPI GGTNACC 1 cut(s) 428
BstSCI CCNGG 7 cut(s) 802, 1414, 1430, 1780, 1794, 1922, 1923
BstSFI CTRYAG 2 cut(s) 1569, 2293
BstSLI GKGCMC 2 cut(s) 57, 988
BstUI CGCG 2 cut(s) 60, 550
BstV2I GAAGAC 2 cut(s) 692, 1110
BstX2I RGATCY 2 cut(s) 1421, 2205
BstYI RGATCY 2 cut(s) 1421, 2205
BtgI CCRYGG 2 cut(s) 308, 2232
BtsCI GGATG 6 cut(s) 460, 469, 662, 718, 1919, 2152
BtsIMutI CAGTG 3 cut(s) 94, 862, 2058
Cac8I GCNNGC 7 cut(s) 293, 599, 1288, 1554, 1683, 1896, 2003
CciI TCATGA 1 cut(s) 502
CfoI GCGC 1 cut(s) 816
Cfr10I RCCGGY 2 cut(s) 1391, 2001
Cfr13I GGNCC 4 cut(s) 21, 200, 1792, 2288
Cfr9I CCCGGG 1 cut(s) 1923
CseI GACGC 1 cut(s) 1994
Csp6I GTAC 7 cut(s) 680, 864, 1167, 1440, 1596, 1688, 2279
CspAI ACCGGT 1 cut(s) 1391
CviQI GTAC 7 cut(s) 680, 864, 1167, 1440, 1596, 1688, 2279
DdeI CTNAG 5 cut(s) 750, 1378, 1548, 1607, 2187
DpnI GATC 9 cut(s) 372, 386, 501, 547, 861, 1002, 1423, 1779, 2207
DpnII GATC 9 cut(s) 370, 384, 499, 545, 859, 1000, 1421, 1777, 2205
EaeI YGGCCR 2 cut(s) 1616, 1999
Eam1104I CTCTTC 2 cut(s) 646, 1378
EarI CTCTTC 2 cut(s) 646, 1378
Ecl136II GAGCTC 2 cut(s) 127, 1873
Eco130I CCWWGG 4 cut(s) 203, 443, 592, 1406
Eco24I GRGCYC 4 cut(s) 33, 129, 1711, 1875
Eco32I GATATC 1 cut(s) 1810
Eco53kI GAGCTC 2 cut(s) 127, 1873
Eco57I CTGAAG 3 cut(s) 316, 704, 1164
Eco88I CYCGRG 2 cut(s) 1889, 1923
Eco91I GGTNACC 1 cut(s) 428
EcoICRI GAGCTC 2 cut(s) 127, 1873
EcoNI CCTNNNNNAGG 1 cut(s) 1892
EcoO109I RGGNCCY 1 cut(s) 2288
EcoO65I GGTNACC 1 cut(s) 428
EcoRI GAATTC 1 cut(s) 2109
EcoRII CCWGG 3 cut(s) 1414, 1780, 1794
EcoRV GATATC 1 cut(s) 1810
EcoT14I CCWWGG 4 cut(s) 203, 443, 592, 1406
EcoT38I GRGCYC 4 cut(s) 33, 129, 1711, 1875
ErhI CCWWGG 4 cut(s) 203, 443, 592, 1406
FalI AAGNNNNNCTT 2 cut(s) 585, 617
FauI CCCGC 2 cut(s) 65, 300
FauNDI CATATG 1 cut(s) 635
FokI GGATG 6 cut(s) 447, 456, 649, 705, 1906, 2139
FriOI GRGCYC 4 cut(s) 33, 129, 1711, 1875
FspBI CTAG 8 cut(s) 219, 228, 486, 867, 927, 1407, 1425, 2118
GlaI GCGC 1 cut(s) 815
GsaI CCCAGC 3 cut(s) 70, 1364, 1659
GsuI CTGGAG 4 cut(s) 251, 1077, 1083, 2093
HapII CCGG 6 cut(s) 803, 1392, 1432, 1876, 1924, 2002
HgaI GACGC 1 cut(s) 1994
HhaI GCGC 1 cut(s) 816
Hin1I GRCGYC 1 cut(s) 2005
Hin6I GCGC 1 cut(s) 814
HinP1I GCGC 1 cut(s) 814
HincII GTYRAC 2 cut(s) 315, 2137
HindII GTYRAC 2 cut(s) 315, 2137
HindIII AAGCTT 1 cut(s) 171
HpaII CCGG 6 cut(s) 803, 1392, 1432, 1876, 1924, 2002
HphI GGTGA 9 cut(s) 422, 660, 1238, 1358, 1523, 1710, 1787, 1832, 1847
Hpy166II GTNNAC 5 cut(s) 315, 986, 1399, 2015, 2137
Hpy188I TCNGA 8 cut(s) 190, 775, 1062, 1278, 1549, 1610, 1978, 2092
Hpy188III TCNNGA 8 cut(s) 473, 497, 503, 907, 1100, 1876, 2072, 2258
Hpy8I GTNNAC 5 cut(s) 315, 986, 1399, 2015, 2137
Hpy99I CGWCG 2 cut(s) 29, 696
HpyAV CCTTC 9 cut(s) 51, 340, 927, 1014, 1098, 1722, 1766, 2166, 2186
HpyCH4III ACNGT 8 cut(s) 89, 312, 379, 832, 1439, 1600, 2012, 2053
HpyCH4IV ACGT 4 cut(s) 694, 1901, 1973, 2139
HpyF10VI GCNNNNNNNGC 9 cut(s) 224, 578, 587, 1264, 1574, 1577, 1580, 1748, 1934
HpyF3I CTNAG 5 cut(s) 750, 1378, 1548, 1607, 2187
HpySE526I ACGT 4 cut(s) 694, 1901, 1973, 2139
Hsp92I GRCGYC 1 cut(s) 2005
HspAI GCGC 1 cut(s) 814
Kpn2I TCCGGA 1 cut(s) 1875
KroI GCCGGC 1 cut(s) 2001
KroNI GCCGGC 1 cut(s) 2003
Kzo9I GATC 9 cut(s) 370, 384, 499, 545, 859, 1000, 1421, 1777, 2205
LmnI GCTCC 8 cut(s) 270, 736, 1026, 1102, 1369, 1648, 1706, 1878
LweI GCATC 6 cut(s) 214, 398, 478, 623, 835, 1642
MaeI CTAG 8 cut(s) 219, 228, 486, 867, 927, 1407, 1425, 2118
MaeII ACGT 4 cut(s) 694, 1901, 1973, 2139
MaeIII GTNAC 7 cut(s) 428, 620, 806, 1226, 1587, 1838, 2006
MalI GATC 9 cut(s) 372, 386, 501, 547, 861, 1002, 1423, 1779, 2207
MboI GATC 9 cut(s) 370, 384, 499, 545, 859, 1000, 1421, 1777, 2205
MboII GAAGA 8 cut(s) 166, 417, 663, 697, 1115, 1365, 1431, 2138
MfeI CAATTG 1 cut(s) 1467
MflI RGATCY 2 cut(s) 1421, 2205
MhlI GDGCHC 7 cut(s) 33, 57, 129, 988, 1645, 1711, 1875
MlsI TGGCCA 1 cut(s) 1618
MluCI AATT 4 cut(s) 1269, 1467, 1845, 2109
MluNI TGGCCA 1 cut(s) 1618
MlyI GAGTC 6 cut(s) 190, 269, 668, 761, 1223, 1529
MmeI TCCRAC 1 cut(s) 2143
Mox20I TGGCCA 1 cut(s) 1618
MroI TCCGGA 1 cut(s) 1875
MroNI GCCGGC 1 cut(s) 2001
MscI TGGCCA 1 cut(s) 1618
MseI TTAA 6 cut(s) 413, 743, 1670, 2022, 2201, 2317
MslI CAYNNNNRTG 2 cut(s) 723, 855
Msp20I TGGCCA 1 cut(s) 1618
MspA1I CMGCKG 1 cut(s) 1577
MspCI CTTAAG 1 cut(s) 2021
MspI CCGG 6 cut(s) 803, 1392, 1432, 1876, 1924, 2002
MspR9I CCNGG 7 cut(s) 804, 1416, 1432, 1782, 1796, 1924, 1925
MunI CAATTG 1 cut(s) 1467
MvaI CCWGG 3 cut(s) 1416, 1782, 1796
MvnI CGCG 2 cut(s) 60, 550
MwoI GCNNNNNNNGC 9 cut(s) 224, 578, 587, 1264, 1574, 1577, 1580, 1748, 1934
NaeI GCCGGC 1 cut(s) 2003
NciI CCSGG 4 cut(s) 804, 1432, 1924, 1925
NdeI CATATG 1 cut(s) 635
NdeII GATC 9 cut(s) 370, 384, 499, 545, 859, 1000, 1421, 1777, 2205
NgoMIV GCCGGC 1 cut(s) 2001
NlaIV GGNNCC 6 cut(s) 1022, 1413, 1436, 1708, 2207, 2290
NmuCI GTSAC 5 cut(s) 428, 620, 1226, 1838, 2006
NspI RCATGY 3 cut(s) 1685, 1755, 2272
PaeI GCATGC 1 cut(s) 1685
PaeR7I CTCGAG 1 cut(s) 1889
PagI TCATGA 1 cut(s) 502
PciI ACATGT 1 cut(s) 2268
PdiI GCCGGC 1 cut(s) 2003
PfeI GAWTC 5 cut(s) 521, 776, 1316, 1491, 1539
PfoI TCCNGGA 2 cut(s) 1414, 1780
PinAI ACCGGT 1 cut(s) 1391
PleI GAGTC 6 cut(s) 189, 268, 667, 761, 1223, 1528
PpsI GAGTC 6 cut(s) 189, 268, 667, 761, 1223, 1528
PscI ACATGT 1 cut(s) 2268
Psp124BI GAGCTC 2 cut(s) 129, 1875
Psp1406I AACGTT 1 cut(s) 1901
Psp6I CCWGG 3 cut(s) 1414, 1780, 1794
PspEI GGTNACC 1 cut(s) 428
PspFI CCCAGC 3 cut(s) 66, 1360, 1655
PspGI CCWGG 3 cut(s) 1414, 1780, 1794
PspN4I GGNNCC 6 cut(s) 1022, 1413, 1436, 1708, 2207, 2290
PspPI GGNCC 4 cut(s) 21, 200, 1792, 2288
PspXI VCTCGAGB 1 cut(s) 1889
PsrI GAACNNNNNNTAC 2 cut(s) 1971, 2003
PstI CTGCAG 1 cut(s) 1573
PsuI RGATCY 2 cut(s) 1421, 2205
RsaI GTAC 7 cut(s) 681, 865, 1168, 1441, 1597, 1689, 2280
RsaNI GTAC 7 cut(s) 680, 864, 1167, 1440, 1596, 1688, 2279
RseI CAYNNNNRTG 2 cut(s) 723, 855
SacI GAGCTC 2 cut(s) 129, 1875
SaqAI TTAA 6 cut(s) 413, 743, 1670, 2022, 2201, 2317
Sau3AI GATC 9 cut(s) 370, 384, 499, 545, 859, 1000, 1421, 1777, 2205
Sau96I GGNCC 4 cut(s) 21, 200, 1792, 2288
ScaI AGTACT 1 cut(s) 681
SchI GAGTC 6 cut(s) 190, 269, 668, 761, 1223, 1529
ScrFI CCNGG 7 cut(s) 804, 1416, 1432, 1782, 1796, 1924, 1925
SduI GDGCHC 7 cut(s) 33, 57, 129, 988, 1645, 1711, 1875
SfaNI GCATC 6 cut(s) 214, 398, 478, 623, 835, 1642
SfcI CTRYAG 2 cut(s) 1569, 2293
Sfr274I CTCGAG 1 cut(s) 1889
SlaI CTCGAG 1 cut(s) 1889
SmaI CCCGGG 1 cut(s) 1925
SmiMI CAYNNNNRTG 2 cut(s) 723, 855
SmlI CTYRAG 2 cut(s) 1889, 2021
SmoI CTYRAG 2 cut(s) 1889, 2021
SpeI ACTAGT 1 cut(s) 866
SphI GCATGC 1 cut(s) 1685
Sse9I AATT 4 cut(s) 1269, 1467, 1845, 2109
SsiI CCGC 9 cut(s) 58, 293, 433, 1018, 1068, 1303, 1577, 1679, 1725
SspI AATATT 2 cut(s) 287, 1501
SspMI CTAG 8 cut(s) 219, 228, 486, 867, 927, 1407, 1425, 2118
SstI GAGCTC 2 cut(s) 129, 1875
StyD4I CCNGG 7 cut(s) 802, 1414, 1430, 1780, 1794, 1922, 1923
StyI CCWWGG 4 cut(s) 203, 443, 592, 1406
TaaI ACNGT 8 cut(s) 89, 312, 379, 832, 1439, 1600, 2012, 2053
TaiI ACGT 4 cut(s) 697, 1904, 1976, 2142
TaqI TCGA 2 cut(s) 782, 1890
TasI AATT 4 cut(s) 1269, 1467, 1845, 2109
TatI WGTACW 1 cut(s) 679
TauI GCSGC 3 cut(s) 1021, 1306, 1580
TfiI GAWTC 5 cut(s) 521, 776, 1316, 1491, 1539
Tru1I TTAA 6 cut(s) 413, 743, 1670, 2022, 2201, 2317
Tru9I TTAA 6 cut(s) 413, 743, 1670, 2022, 2201, 2317
TscAI CASTG 3 cut(s) 94, 862, 2058
TseFI GTSAC 5 cut(s) 428, 620, 1226, 1838, 2006
Tsp45I GTSAC 5 cut(s) 428, 620, 1226, 1838, 2006
TspDTI ATGAA 8 cut(s) 449, 519, 707, 909, 1068, 2141, 2277, 2289
TspGWI ACGGA 4 cut(s) 40, 133, 1110, 1626
TspMI CCCGGG 1 cut(s) 1923
TspRI CASTG 3 cut(s) 94, 862, 2058
Vha464I CTTAAG 1 cut(s) 2021
VneI GTGCAC 1 cut(s) 984
XagI CCTNNNNNAGG 1 cut(s) 1892
XapI RAATTY 1 cut(s) 2109
XceI RCATGY 3 cut(s) 1685, 1755, 2272
XcmI CCANNNNNNNNNTGG 1 cut(s) 909
XhoI CTCGAG 1 cut(s) 1889
XmaI CCCGGG 1 cut(s) 1923
XmaJI CCTAGG 1 cut(s) 1406
XspI CTAG 8 cut(s) 219, 228, 486, 867, 927, 1407, 1425, 2118
ZrmI AGTACT 1 cut(s) 681
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.