FvH4_2g26210

RNA recognition motif 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001927
Physical Location & Seq
Reverse (-)
21080207 .. 21081633
1427 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g26210.t1

Sequence Viewer

Length: 969 bp
ATGTCTCGCAGACCTCTTAACCCATATGCCCAACCCTACTTCGGTCACCATTTTCATCCCAATCAAAGCTTTCTGTATGAAGTCCCAACACATGCCTATGTTCCAAATCTCTACTCTATGCAAAACCCAACCTTGTATCCAGACCACTACTCACACATGGAAACTACTGCTGCTATGGATCAAAACCCCAGAGCAGTTGTTCCGAGGAGAAAGAGGAAGAACTTCTGGCGAGAGAGGAACGTGGGTCCTAGGTTGAGGTCGTCACAGCCGAGATTGGCTGAGAATCAAGCTTCAAACTTTGCATCAGCTAGTAACTGGGGTGGTACTGAAGTTCCTTCTTCTTCAAGACCTGGCAGGAAAAACAGAAGCCGAAGAAGGAAGGTGGGAAGACTGAGGCCCAGTCTTGGCCATGGTGTTCAAGCTTCTAGCTTTGCCAATGGCAAGGGTTCTGTGATTCCTTTTCCTGGAAGTCCTGATGAGCAAAAAGGGTCTGAGGTTACTACTCTTATGATTAAGAATATCCCTTGTAAGCTTAGAAGAAGCGATTTGCTGCTCAAGTTGAAAGAGCATTGCCAAGAGGAGAACAAAAAGGCTGATTCTTTGCAAAAATCGGAGATCAATTTCTTCTATTTACCACTGGATTTCAAAAAATTTTGGGAAAAGAAAACTATTGCAAATCTTGGGTATGCGTTTGTGAACTTCACCACTGCTGTTGGAGCCCTGAGGTTCTATGCGAGATACGACAAGTCTATGTGGAAGGAGGTCACTCAGAGCACCAAAGTCTGCGAGATAACTTGCGCCAAGATCCAGGGTTTCGACGCATTGAGGAAAACGTTTAGTGGCAGGAGATACCATGGTCTCACAAATGAGTTTTTGCCTGTGATCTTGGCTCCTGCTTGTGATGGAGTGAGGGAGTCCAACCACATAACTGTAGGACGACTTCTGGATGCACCAGAGATTGAAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_2

Protein Analysis

323

Amino Acids

36.79

Weight (kDa)

9.91

Isoelectric Point (pI)

60.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_2 PF04059 167 - 279 7.1e-13 RNA recognition motif 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 833
AclWI GGATC 2 cut(s) 186, 799
AcoI YGGCCR 1 cut(s) 406
AcsI RAATTY 1 cut(s) 650
AcuI CTGAAG 1 cut(s) 348
AfaI GTAC 1 cut(s) 325
AfiI CCNNNNNNNGG 3 cut(s) 41, 404, 464
AgsI TTSAA 6 cut(s) 294, 345, 419, 562, 646, 962
AjnI CCWGG 3 cut(s) 349, 463, 807
AluBI AGCT 6 cut(s) 69, 290, 308, 422, 429, 532
AluI AGCT 6 cut(s) 69, 290, 308, 422, 429, 532
Alw21I GWGCWC 1 cut(s) 776
Alw26I GTCTC 2 cut(s) 9, 863
AlwI GGATC 2 cut(s) 186, 799
AoxI GGCC 2 cut(s) 395, 406
ApeKI GCWGC 2 cut(s) 170, 550
ApoI RAATTY 1 cut(s) 650
Asp700I GAANNNNTTC 1 cut(s) 221
AspA2I CCTAGG 1 cut(s) 248
AspLEI GCGC 1 cut(s) 800
AspS9I GGNCC 2 cut(s) 245, 396
AsuHPI GGTGA 2 cut(s) 38, 694
AvaII GGWCC 1 cut(s) 245
AvrII CCTAGG 1 cut(s) 248
AxyI CCTNAGG 1 cut(s) 722
BaeI ACNNNNGTAYC 4 cut(s) 315, 348, 841, 874
BalI TGGCCA 1 cut(s) 408
BanII GRGCYC 1 cut(s) 721
BarI GAAGNNNNNNTAC 2 cut(s) 924, 956
BbsI GAAGAC 1 cut(s) 394
Bbv12I GWGCWC 1 cut(s) 776
BbvI GCAGC 2 cut(s) 157, 537
BccI CCATC 1 cut(s) 896
BciT130I CCWGG 3 cut(s) 351, 465, 809
BciVI GTATCC 1 cut(s) 147
BcoDI GTCTC 2 cut(s) 9, 863
BfaI CTAG 3 cut(s) 249, 309, 426
BfmI CTRYAG 1 cut(s) 930
BfuI GTATCC 1 cut(s) 147
BisI GCNGC 2 cut(s) 171, 551
BlnI CCTAGG 1 cut(s) 248
BlsI GCNGC 2 cut(s) 172, 552
Bme1390I CCNGG 3 cut(s) 351, 465, 809
Bme18I GGWCC 1 cut(s) 245
BmgT120I GGNCC 2 cut(s) 245, 396
BmiI GGNNCC 3 cut(s) 246, 718, 891
BmrFI CCNGG 3 cut(s) 351, 465, 809
BmrI ACTGGG 2 cut(s) 325, 393
BmsI GCATC 2 cut(s) 311, 937
BmuI ACTGGG 2 cut(s) 325, 393
BpiI GAAGAC 1 cut(s) 394
BpuEI CTTGAG 1 cut(s) 539
BsaI GGTCTC 1 cut(s) 863
BsaJI CCNNGG 5 cut(s) 203, 248, 409, 808, 853
Bsc4I CCNNNNNNNGG 3 cut(s) 41, 404, 464
Bse1I ACTGG 3 cut(s) 320, 399, 642
Bse21I CCTNAGG 1 cut(s) 722
Bse3DI GCAATG 1 cut(s) 568
BseBI CCWGG 3 cut(s) 351, 465, 809
BseDI CCNNGG 5 cut(s) 203, 248, 409, 808, 853
BseGI GGATG 2 cut(s) 55, 952
BseLI CCNNNNNNNGG 3 cut(s) 41, 404, 464
BseMI GCAATG 1 cut(s) 568
BseMII CTCAG 5 cut(s) 270, 383, 483, 713, 782
BseNI ACTGG 3 cut(s) 320, 399, 642
BseRI GAGGAG 2 cut(s) 220, 593
BseXI GCAGC 2 cut(s) 157, 537
BshFI GGCC 2 cut(s) 397, 408
BsiHKAI GWGCWC 1 cut(s) 776
BslFI GGGAC 1 cut(s) 68
BslI CCNNNNNNNGG 3 cut(s) 41, 404, 464
BsmAI GTCTC 2 cut(s) 9, 863
BsmFI GGGAC 1 cut(s) 68
BsnI GGCC 2 cut(s) 397, 408
Bso31I GGTCTC 1 cut(s) 863
Bsp1286I GDGCHC 2 cut(s) 721, 776
Bsp143I GATC 4 cut(s) 178, 615, 804, 882
Bsp19I CCATGG 2 cut(s) 409, 853
BspANI GGCC 2 cut(s) 397, 408
BspCNI CTCAG 5 cut(s) 271, 384, 484, 714, 781
BspLI GGNNCC 3 cut(s) 246, 718, 891
BspPI GGATC 2 cut(s) 186, 799
BspTNI GGTCTC 1 cut(s) 863
BsrDI GCAATG 1 cut(s) 568
BsrI ACTGG 3 cut(s) 320, 399, 642
BssECI CCNNGG 5 cut(s) 203, 248, 409, 808, 853
BssMI GATC 4 cut(s) 178, 615, 804, 882
BssT1I CCWWGG 3 cut(s) 248, 409, 853
Bst2UI CCWGG 3 cut(s) 351, 465, 809
Bst4CI ACNGT 1 cut(s) 931
BstDEI CTNAG 6 cut(s) 279, 392, 492, 533, 722, 768
BstDSI CCRYGG 2 cut(s) 409, 853
BstEII GGTNACC 1 cut(s) 44
BstF5I GGATG 2 cut(s) 55, 952
BstHHI GCGC 1 cut(s) 800
BstKTI GATC 4 cut(s) 181, 618, 807, 885
BstMAI GTCTC 2 cut(s) 9, 863
BstMBI GATC 4 cut(s) 178, 615, 804, 882
BstMWI GCNNNNNNNGC 1 cut(s) 716
BstNI CCWGG 3 cut(s) 351, 465, 809
BstNSI RCATGY 1 cut(s) 95
BstPI GGTNACC 1 cut(s) 44
BstSCI CCNGG 3 cut(s) 349, 463, 807
BstSFI CTRYAG 1 cut(s) 930
BstV1I GCAGC 2 cut(s) 157, 537
BstV2I GAAGAC 1 cut(s) 394
BstX2I RGATCY 1 cut(s) 804
BstYI RGATCY 1 cut(s) 804
Bsu36I CCTNAGG 1 cut(s) 722
BsuI GTATCC 1 cut(s) 147
BsuRI GGCC 2 cut(s) 397, 408
BtgI CCRYGG 2 cut(s) 409, 853
BtsCI GGATG 2 cut(s) 55, 952
BtsI GCAGTG 1 cut(s) 705
BtsIMutI CAGTG 2 cut(s) 635, 705
CfoI GCGC 1 cut(s) 800
Cfr13I GGNCC 2 cut(s) 245, 396
CseI GACGC 1 cut(s) 827
Csp6I GTAC 1 cut(s) 324
CspCI CAANNNNNGTGG 2 cut(s) 694, 729
CviAII CATG 4 cut(s) 92, 157, 410, 854
CviQI GTAC 1 cut(s) 324
DdeI CTNAG 6 cut(s) 279, 392, 492, 533, 722, 768
DpnI GATC 4 cut(s) 180, 617, 806, 884
DpnII GATC 4 cut(s) 178, 615, 804, 882
EaeI YGGCCR 1 cut(s) 406
Eco130I CCWWGG 3 cut(s) 248, 409, 853
Eco24I GRGCYC 1 cut(s) 721
Eco31I GGTCTC 1 cut(s) 863
Eco47I GGWCC 1 cut(s) 245
Eco57I CTGAAG 1 cut(s) 348
Eco81I CCTNAGG 1 cut(s) 722
Eco91I GGTNACC 1 cut(s) 44
EcoO109I RGGNCCY 1 cut(s) 245
EcoO65I GGTNACC 1 cut(s) 44
EcoRII CCWGG 3 cut(s) 349, 463, 807
EcoT14I CCWWGG 3 cut(s) 248, 409, 853
EcoT38I GRGCYC 1 cut(s) 721
ErhI CCWWGG 3 cut(s) 248, 409, 853
FaeI CATG 4 cut(s) 95, 160, 413, 857
FaqI GGGAC 1 cut(s) 68
FatI CATG 4 cut(s) 91, 156, 409, 853
FauNDI CATATG 1 cut(s) 25
Fnu4HI GCNGC 2 cut(s) 171, 551
FokI GGATG 2 cut(s) 42, 959
FriOI GRGCYC 1 cut(s) 721
Fsp4HI GCNGC 2 cut(s) 171, 551
FspBI CTAG 3 cut(s) 249, 309, 426
GlaI GCGC 1 cut(s) 799
GluI GCNGC 2 cut(s) 171, 551
HaeIII GGCC 2 cut(s) 397, 408
HgaI GACGC 1 cut(s) 827
HhaI GCGC 1 cut(s) 800
Hin1II CATG 4 cut(s) 95, 160, 413, 857
Hin6I GCGC 1 cut(s) 798
HinP1I GCGC 1 cut(s) 798
HindIII AAGCTT 4 cut(s) 67, 288, 420, 530
HinfI GANTC 4 cut(s) 283, 454, 596, 914
HphI GGTGA 2 cut(s) 38, 694
Hpy166II GTNNAC 1 cut(s) 697
Hpy188I TCNGA 4 cut(s) 204, 493, 613, 771
Hpy188III TCNNGA 4 cut(s) 140, 345, 473, 944
Hpy8I GTNNAC 1 cut(s) 697
Hpy99I CGWCG 1 cut(s) 821
HpyAV CCTTC 4 cut(s) 345, 369, 373, 751
HpyCH4III ACNGT 1 cut(s) 931
HpyCH4IV ACGT 2 cut(s) 240, 833
HpyCH4V TGCA 5 cut(s) 121, 302, 604, 674, 950
HpyF10VI GCNNNNNNNGC 1 cut(s) 716
HpyF3I CTNAG 6 cut(s) 279, 392, 492, 533, 722, 768
HpySE526I ACGT 2 cut(s) 240, 833
Hsp92II CATG 4 cut(s) 95, 160, 413, 857
HspAI GCGC 1 cut(s) 798
Kzo9I GATC 4 cut(s) 178, 615, 804, 882
LmnI GCTCC 2 cut(s) 716, 895
Lsp1109I GCAGC 2 cut(s) 157, 537
LweI GCATC 2 cut(s) 311, 937
MaeI CTAG 3 cut(s) 249, 309, 426
MaeII ACGT 2 cut(s) 240, 833
MaeIII GTNAC 5 cut(s) 44, 261, 311, 496, 763
MalI GATC 4 cut(s) 180, 617, 806, 884
MboI GATC 4 cut(s) 178, 615, 804, 882
MboII GAAGA 7 cut(s) 229, 330, 333, 384, 399, 549, 616
MflI RGATCY 1 cut(s) 804
MhlI GDGCHC 2 cut(s) 721, 776
MlsI TGGCCA 1 cut(s) 408
MluCI AATT 2 cut(s) 619, 650
MluNI TGGCCA 1 cut(s) 408
MlyI GAGTC 1 cut(s) 923
MmeI TCCRAC 2 cut(s) 694, 942
Mox20I TGGCCA 1 cut(s) 408
MroXI GAANNNNTTC 1 cut(s) 221
MscI TGGCCA 1 cut(s) 408
MseI TTAA 2 cut(s) 18, 513
MslI CAYNNNNRTG 1 cut(s) 96
Msp20I TGGCCA 1 cut(s) 408
MspR9I CCNGG 3 cut(s) 351, 465, 809
MvaI CCWGG 3 cut(s) 351, 465, 809
MwoI GCNNNNNNNGC 1 cut(s) 716
NcoI CCATGG 2 cut(s) 409, 853
NdeI CATATG 1 cut(s) 25
NdeII GATC 4 cut(s) 178, 615, 804, 882
NlaIII CATG 4 cut(s) 95, 160, 413, 857
NlaIV GGNNCC 3 cut(s) 246, 718, 891
NmeAIII GCCGAG 1 cut(s) 294
NmuCI GTSAC 3 cut(s) 44, 261, 763
NspI RCATGY 1 cut(s) 95
PcsI WCGNNNNNNNCGW 1 cut(s) 266
PdmI GAANNNNTTC 1 cut(s) 221
PfeI GAWTC 3 cut(s) 283, 454, 596
PfoI TCCNGGA 1 cut(s) 463
PkrI GCNGC 2 cut(s) 172, 552
PleI GAGTC 1 cut(s) 922
PpsI GAGTC 1 cut(s) 922
PpuMI RGGWCCY 1 cut(s) 245
Psp1406I AACGTT 1 cut(s) 833
Psp5II RGGWCCY 1 cut(s) 245
Psp6I CCWGG 3 cut(s) 349, 463, 807
PspEI GGTNACC 1 cut(s) 44
PspGI CCWGG 3 cut(s) 349, 463, 807
PspN4I GGNNCC 3 cut(s) 246, 718, 891
PspPI GGNCC 2 cut(s) 245, 396
PspPPI RGGWCCY 1 cut(s) 245
PsuI RGATCY 1 cut(s) 804
RsaI GTAC 1 cut(s) 325
RsaNI GTAC 1 cut(s) 324
RseI CAYNNNNRTG 1 cut(s) 96
SaqAI TTAA 2 cut(s) 18, 513
SatI GCNGC 2 cut(s) 171, 551
Sau3AI GATC 4 cut(s) 178, 615, 804, 882
Sau96I GGNCC 2 cut(s) 245, 396
SchI GAGTC 1 cut(s) 923
ScrFI CCNGG 3 cut(s) 351, 465, 809
SduI GDGCHC 2 cut(s) 721, 776
SfaNI GCATC 2 cut(s) 311, 937
SfcI CTRYAG 1 cut(s) 930
SinI GGWCC 1 cut(s) 245
SmiMI CAYNNNNRTG 1 cut(s) 96
SmlI CTYRAG 1 cut(s) 554
SmoI CTYRAG 1 cut(s) 554
Sse9I AATT 2 cut(s) 619, 650
SspMI CTAG 3 cut(s) 249, 309, 426
StyD4I CCNGG 3 cut(s) 349, 463, 807
StyI CCWWGG 3 cut(s) 248, 409, 853
TaaI ACNGT 1 cut(s) 931
TaiI ACGT 2 cut(s) 243, 836
TaqI TCGA 1 cut(s) 816
TaqII GACCGA 1 cut(s) 32
TasI AATT 2 cut(s) 619, 650
TfiI GAWTC 3 cut(s) 283, 454, 596
Tru1I TTAA 2 cut(s) 18, 513
Tru9I TTAA 2 cut(s) 18, 513
TscAI CASTG 2 cut(s) 642, 712
TseFI GTSAC 3 cut(s) 44, 261, 763
TseI GCWGC 2 cut(s) 170, 550
Tsp45I GTSAC 3 cut(s) 44, 261, 763
TspDTI ATGAA 2 cut(s) 44, 93
TspRI CASTG 2 cut(s) 642, 712
VpaK11BI GGWCC 1 cut(s) 245
XapI RAATTY 1 cut(s) 650
XceI RCATGY 1 cut(s) 95
XmaJI CCTAGG 1 cut(s) 248
XmnI GAANNNNTTC 1 cut(s) 221
XspI CTAG 3 cut(s) 249, 309, 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.