MD08G1171900.v1.1

RNA recognition motif 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0001927
Physical Location & Seq
Forward (+)
20642786 .. 20644286
1501 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1171900.v1.1.491

Sequence Viewer

Length: 921 bp
ATGGGTTCGAAAGCTCTAAATCCTAAGGCTCAGCCTTTTTTCTTGAACCGTTTTCAGACGCCTTATCTTCCTGCTTGGCCTGTATGCGCATATCGAATCGTTCATCAGCCTAGATCCTTTTTTTGCCAACTGAACTTGGTCCAACCCAACCCAACTACAAAAAGGGTGCCGAGAAAGAGTGTTGAGAGTAAGAGGAATTTTGCTCCACCAAGGTCCAGAAACCCTTTGAAGAAGCTTGAAGATGAGAACCATGTAGTGGGTGCTCCTAGGGTTCTCGCCTGGGTGCCCAAGAAAGATCTGCCGGCTGGGAAGAGGTATACAGCGTCGTATCGGAAGATAAAGAAAGATACTGTGCGCGGCTGTGATGATGGCGGTGGCAGTGGCGGTGGTTGTGTAATTCCGTTTCCTGCAAGTCCTGATCTGGAAATTAGCTCCGGTCCTACCACGATCATGATAAAAAACATCCCAAACCAATTTCAGAGGTGTGATTTGCTGACCGTCCTGAGAAATCACTGCCGTGATGAGAACCTACAGGCTTGCACCGAAGGTGTTACCACGAAATCTGAGTTCGATTTTGTTTATCTTCCAATGGATTTTAAGCAATTTTGGAACCACAAGAGGACTGCCAATCTGGGCTATGCCTTTGTTAATTTCACCAGTTTTACTGCTGCAAAGAGATTTTACAAGAAGTTTCACAATGAACAATGGAAGGTCCGCTGCAACAAGAAAACATGTGAAATCACTTGCGCTAAGGTTCAGGGGCTCGAAGCACTGAAGAAGAACTTTGAGAGTAAAGTGTTTAGGTGCCACTCGGAGGAGTATTTGCCTGCGGTGGTGGAACCTCCATGTGACGGTGTTCTGCAGCAGCCCAAAGTCACTTCGGTGGGGACACGTGTAGGGTTGCCCTTTGTAGATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_2

Protein Analysis

307

Amino Acids

34.8

Weight (kDa)

9.64

Isoelectric Point (pI)

47.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_2 PF04059 148 - 262 9.5e-14 RNA recognition motif 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 88
AccB1I GGYRCC 3 cut(s) 166, 283, 804
AccB7I CCANNNNNTGG 1 cut(s) 256
AccI GTMKAC 1 cut(s) 317
AccII CGCG 1 cut(s) 357
AciI CCGC 5 cut(s) 357, 372, 384, 715, 830
AclWI GGATC 1 cut(s) 108
AcsI RAATTY 1 cut(s) 196
AcuI CTGAAG 1 cut(s) 794
AcvI CACGTG 1 cut(s) 893
AcyI GRCGYC 1 cut(s) 59
AfiI CCNNNNNNNGG 3 cut(s) 256, 814, 851
AflIII ACRYGT 3 cut(s) 731, 890, 892
AgsI TTSAA 3 cut(s) 46, 229, 239
AjnI CCWGG 1 cut(s) 278
AleI CACNNNNGTG 2 cut(s) 516, 881
AluBI AGCT 3 cut(s) 14, 235, 432
AluI AGCT 3 cut(s) 14, 235, 432
Alw21I GWGCWC 1 cut(s) 265
AlwI GGATC 1 cut(s) 108
AoxI GGCC 1 cut(s) 77
ApeKI GCWGC 4 cut(s) 668, 717, 862, 865
ApoI RAATTY 1 cut(s) 196
AspA2I CCTAGG 1 cut(s) 266
AspLEI GCGC 3 cut(s) 89, 357, 749
AspS9I GGNCC 4 cut(s) 139, 213, 437, 712
AsuHPI GGTGA 1 cut(s) 646
AsuII TTCGAA 1 cut(s) 8
AvaII GGWCC 4 cut(s) 139, 213, 437, 712
AvrII CCTAGG 1 cut(s) 266
AxyI CCTNAGG 1 cut(s) 24
BaeGI GKGCMC 1 cut(s) 288
BanI GGYRCC 3 cut(s) 166, 283, 804
BanII GRGCYC 1 cut(s) 765
BbrPI CACGTG 1 cut(s) 893
Bbv12I GWGCWC 1 cut(s) 265
BbvI GCAGC 4 cut(s) 655, 704, 874, 877
BccI CCATC 1 cut(s) 362
BceAI ACGGC 1 cut(s) 501
BciT130I CCWGG 1 cut(s) 280
BfaI CTAG 2 cut(s) 111, 267
BfmI CTRYAG 2 cut(s) 530, 860
BglII AGATCT 1 cut(s) 295
BisI GCNGC 5 cut(s) 358, 669, 718, 863, 866
BlnI CCTAGG 1 cut(s) 266
BlpI GCTNAGC 1 cut(s) 30
BlsI GCNGC 5 cut(s) 359, 670, 719, 864, 867
Bme1390I CCNGG 1 cut(s) 280
Bme18I GGWCC 4 cut(s) 139, 213, 437, 712
BmgT120I GGNCC 4 cut(s) 139, 213, 437, 712
BmiI GGNNCC 5 cut(s) 168, 285, 611, 806, 840
BmrFI CCNGG 1 cut(s) 280
Bpu10I CCTNAGC 1 cut(s) 750
Bpu1102I GCTNAGC 1 cut(s) 30
Bpu14I TTCGAA 1 cut(s) 8
BsaAI YACGTR 1 cut(s) 893
BsaHI GRCGYC 1 cut(s) 59
BsaJI CCNNGG 3 cut(s) 209, 266, 279
BsaWI WCCGGW 1 cut(s) 434
Bsc4I CCNNNNNNNGG 3 cut(s) 256, 814, 851
Bse118I RCCGGY 1 cut(s) 301
Bse1I ACTGG 1 cut(s) 657
Bse21I CCTNAGG 1 cut(s) 24
BseBI CCWGG 1 cut(s) 280
BseDI CCNNGG 3 cut(s) 209, 266, 279
BseGI GGATG 1 cut(s) 462
BseLI CCNNNNNNNGG 3 cut(s) 256, 814, 851
BseMII CTCAG 3 cut(s) 44, 494, 555
BseNI ACTGG 1 cut(s) 657
BseRI GAGGAG 1 cut(s) 830
BseSI GKGCMC 1 cut(s) 288
BseXI GCAGC 4 cut(s) 655, 704, 874, 877
BseYI CCCAGC 1 cut(s) 305
Bsh1236I CGCG 1 cut(s) 357
BshFI GGCC 1 cut(s) 79
BshNI GGYRCC 3 cut(s) 166, 283, 804
BsiHKAI GWGCWC 1 cut(s) 265
BsiSI CCGG 2 cut(s) 302, 435
BslFI GGGAC 1 cut(s) 901
BslI CCNNNNNNNGG 3 cut(s) 256, 814, 851
BsmFI GGGAC 1 cut(s) 901
BsnI GGCC 1 cut(s) 79
Bsp119I TTCGAA 1 cut(s) 8
Bsp1286I GDGCHC 3 cut(s) 265, 288, 765
Bsp143I GATC 4 cut(s) 113, 295, 418, 447
Bsp1720I GCTNAGC 1 cut(s) 30
BspACI CCGC 5 cut(s) 357, 372, 384, 715, 830
BspANI GGCC 1 cut(s) 79
BspCNI CTCAG 3 cut(s) 43, 495, 556
BspFNI CGCG 1 cut(s) 357
BspHI TCATGA 1 cut(s) 450
BspLI GGNNCC 5 cut(s) 168, 285, 611, 806, 840
BspMAI CTGCAG 1 cut(s) 864
BspPI GGATC 1 cut(s) 108
BspT104I TTCGAA 1 cut(s) 8
BspT107I GGYRCC 3 cut(s) 166, 283, 804
BsrFI RCCGGY 1 cut(s) 301
BsrI ACTGG 1 cut(s) 657
BssAI RCCGGY 1 cut(s) 301
BssECI CCNNGG 3 cut(s) 209, 266, 279
BssMI GATC 4 cut(s) 113, 295, 418, 447
BssNAI GTATAC 1 cut(s) 318
BssNI GRCGYC 1 cut(s) 59
BssT1I CCWWGG 2 cut(s) 209, 266
Bst1107I GTATAC 1 cut(s) 318
Bst2UI CCWGG 1 cut(s) 280
Bst4CI ACNGT 4 cut(s) 50, 352, 499, 854
Bst6I CTCTTC 1 cut(s) 305
BstACI GRCGYC 1 cut(s) 59
BstBAI YACGTR 1 cut(s) 893
BstBI TTCGAA 1 cut(s) 8
BstC8I GCNNGC 3 cut(s) 303, 538, 828
BstDEI CTNAG 5 cut(s) 24, 30, 503, 564, 750
BstF5I GGATG 1 cut(s) 462
BstFNI CGCG 1 cut(s) 357
BstHHI GCGC 3 cut(s) 89, 357, 749
BstKTI GATC 4 cut(s) 116, 298, 421, 450
BstMBI GATC 4 cut(s) 113, 295, 418, 447
BstNI CCWGG 1 cut(s) 280
BstNSI RCATGY 1 cut(s) 735
BstSCI CCNGG 1 cut(s) 278
BstSFI CTRYAG 2 cut(s) 530, 860
BstSLI GKGCMC 1 cut(s) 288
BstUI CGCG 1 cut(s) 357
BstV1I GCAGC 4 cut(s) 655, 704, 874, 877
BstX2I RGATCY 2 cut(s) 113, 295
BstYI RGATCY 2 cut(s) 113, 295
BstZ17I GTATAC 1 cut(s) 318
Bsu36I CCTNAGG 1 cut(s) 24
BsuRI GGCC 1 cut(s) 79
BtsCI GGATG 1 cut(s) 462
BtsI GCAGTG 2 cut(s) 385, 511
BtsIMutI CAGTG 3 cut(s) 385, 511, 770
Cac8I GCNNGC 3 cut(s) 303, 538, 828
CciI TCATGA 1 cut(s) 450
CfoI GCGC 3 cut(s) 89, 357, 749
Cfr10I RCCGGY 1 cut(s) 301
Cfr13I GGNCC 4 cut(s) 139, 213, 437, 712
CseI GACGC 2 cut(s) 67, 312
CviAII CATG 4 cut(s) 251, 451, 732, 846
DdeI CTNAG 5 cut(s) 24, 30, 503, 564, 750
DpnI GATC 4 cut(s) 115, 297, 420, 449
DpnII GATC 4 cut(s) 113, 295, 418, 447
Eam1104I CTCTTC 1 cut(s) 305
EarI CTCTTC 1 cut(s) 305
Eco130I CCWWGG 2 cut(s) 209, 266
Eco24I GRGCYC 1 cut(s) 765
Eco47I GGWCC 4 cut(s) 139, 213, 437, 712
Eco57I CTGAAG 1 cut(s) 794
Eco72I CACGTG 1 cut(s) 893
Eco81I CCTNAGG 1 cut(s) 24
EcoRII CCWGG 1 cut(s) 278
EcoT14I CCWWGG 2 cut(s) 209, 266
EcoT38I GRGCYC 1 cut(s) 765
ErhI CCWWGG 2 cut(s) 209, 266
FaeI CATG 4 cut(s) 254, 454, 735, 849
FaiI YATR 8 cut(s) 85, 91, 252, 318, 452, 639, 733, 847
FalI AAGNNNNNCTT 2 cut(s) 767, 799
FaqI GGGAC 1 cut(s) 901
FatI CATG 4 cut(s) 250, 450, 731, 845
FblI GTMKAC 1 cut(s) 317
Fnu4HI GCNGC 5 cut(s) 358, 669, 718, 863, 866
FokI GGATG 1 cut(s) 449
FriOI GRGCYC 1 cut(s) 765
Fsp4HI GCNGC 5 cut(s) 358, 669, 718, 863, 866
FspAI RTGCGCAY 1 cut(s) 88
FspBI CTAG 2 cut(s) 111, 267
FspI TGCGCA 1 cut(s) 88
GlaI GCGC 3 cut(s) 88, 356, 748
GluI GCNGC 5 cut(s) 358, 669, 718, 863, 866
GsaI CCCAGC 1 cut(s) 309
HaeIII GGCC 1 cut(s) 79
HapII CCGG 2 cut(s) 302, 435
HgaI GACGC 2 cut(s) 67, 312
HhaI GCGC 3 cut(s) 89, 357, 749
Hin1I GRCGYC 1 cut(s) 59
Hin1II CATG 4 cut(s) 254, 454, 735, 849
Hin6I GCGC 3 cut(s) 87, 355, 747
HinP1I GCGC 3 cut(s) 87, 355, 747
HindIII AAGCTT 1 cut(s) 233
HinfI GANTC 1 cut(s) 96
HpaII CCGG 2 cut(s) 302, 435
HphI GGTGA 1 cut(s) 646
Hpy166II GTNNAC 1 cut(s) 318
Hpy188I TCNGA 5 cut(s) 57, 333, 480, 565, 814
Hpy188III TCNNGA 6 cut(s) 43, 216, 416, 422, 451, 502
Hpy8I GTNNAC 1 cut(s) 318
Hpy99I CGWCG 1 cut(s) 328
HpyAV CCTTC 2 cut(s) 539, 703
HpyCH4III ACNGT 4 cut(s) 50, 352, 499, 854
HpyCH4IV ACGT 1 cut(s) 892
HpyCH4V TGCA 5 cut(s) 410, 540, 671, 720, 862
HpyF3I CTNAG 5 cut(s) 24, 30, 503, 564, 750
HpySE526I ACGT 1 cut(s) 892
Hsp92I GRCGYC 1 cut(s) 59
Hsp92II CATG 4 cut(s) 254, 454, 735, 849
HspAI GCGC 3 cut(s) 87, 355, 747
KroI GCCGGC 1 cut(s) 301
KroNI GCCGGC 1 cut(s) 303
Kzo9I GATC 4 cut(s) 113, 295, 418, 447
LmnI GCTCC 3 cut(s) 208, 268, 437
Lsp1109I GCAGC 4 cut(s) 655, 704, 874, 877
MaeI CTAG 2 cut(s) 111, 267
MaeII ACGT 1 cut(s) 892
MaeIII GTNAC 3 cut(s) 550, 848, 874
MalI GATC 4 cut(s) 115, 297, 420, 449
MboI GATC 4 cut(s) 113, 295, 418, 447
MboII GAAGA 8 cut(s) 59, 241, 251, 322, 346, 575, 787, 790
MflI RGATCY 2 cut(s) 113, 295
MhlI GDGCHC 3 cut(s) 265, 288, 765
MluCI AATT 6 cut(s) 196, 396, 426, 473, 602, 649
MmeI TCCRAC 1 cut(s) 166
MnlI CCTC 6 cut(s) 186, 306, 474, 612, 808, 852
MroNI GCCGGC 1 cut(s) 301
MseI TTAA 2 cut(s) 597, 648
MslI CAYNNNNRTG 3 cut(s) 449, 516, 881
MspA1I CMGCKG 1 cut(s) 717
MspI CCGG 2 cut(s) 302, 435
MspR9I CCNGG 1 cut(s) 280
MvaI CCWGG 1 cut(s) 280
MvnI CGCG 1 cut(s) 357
NaeI GCCGGC 1 cut(s) 303
NdeII GATC 4 cut(s) 113, 295, 418, 447
NgoMIV GCCGGC 1 cut(s) 301
NlaIII CATG 4 cut(s) 254, 454, 735, 849
NlaIV GGNNCC 5 cut(s) 168, 285, 611, 806, 840
NmeAIII GCCGAG 1 cut(s) 195
NmuCI GTSAC 2 cut(s) 848, 874
NsbI TGCGCA 1 cut(s) 88
NspI RCATGY 1 cut(s) 735
NspV TTCGAA 1 cut(s) 8
OliI CACNNNNGTG 2 cut(s) 516, 881
PagI TCATGA 1 cut(s) 450
PciI ACATGT 1 cut(s) 731
PdiI GCCGGC 1 cut(s) 303
PfeI GAWTC 1 cut(s) 96
PflMI CCANNNNNTGG 1 cut(s) 256
PkrI GCNGC 5 cut(s) 359, 670, 719, 864, 867
PmaCI CACGTG 1 cut(s) 893
PmlI CACGTG 1 cut(s) 893
Ppu21I YACGTR 1 cut(s) 893
PscI ACATGT 1 cut(s) 731
Psp6I CCWGG 1 cut(s) 278
PspCI CACGTG 1 cut(s) 893
PspFI CCCAGC 1 cut(s) 305
PspGI CCWGG 1 cut(s) 278
PspN4I GGNNCC 5 cut(s) 168, 285, 611, 806, 840
PspPI GGNCC 4 cut(s) 139, 213, 437, 712
PstI CTGCAG 1 cut(s) 864
PsuI RGATCY 2 cut(s) 113, 295
RseI CAYNNNNRTG 3 cut(s) 449, 516, 881
SaqAI TTAA 2 cut(s) 597, 648
SatI GCNGC 5 cut(s) 358, 669, 718, 863, 866
Sau3AI GATC 4 cut(s) 113, 295, 418, 447
Sau96I GGNCC 4 cut(s) 139, 213, 437, 712
ScrFI CCNGG 1 cut(s) 280
SduI GDGCHC 3 cut(s) 265, 288, 765
SfcI CTRYAG 2 cut(s) 530, 860
SfuI TTCGAA 1 cut(s) 8
SinI GGWCC 4 cut(s) 139, 213, 437, 712
SmiMI CAYNNNNRTG 3 cut(s) 449, 516, 881
Sse9I AATT 6 cut(s) 196, 396, 426, 473, 602, 649
SsiI CCGC 5 cut(s) 357, 372, 384, 715, 830
SspMI CTAG 2 cut(s) 111, 267
StyD4I CCNGG 1 cut(s) 278
StyI CCWWGG 2 cut(s) 209, 266
TaaI ACNGT 4 cut(s) 50, 352, 499, 854
TaiI ACGT 1 cut(s) 895
TaqI TCGA 4 cut(s) 8, 94, 570, 765
TasI AATT 6 cut(s) 196, 396, 426, 473, 602, 649
TauI GCSGC 1 cut(s) 360
TfiI GAWTC 1 cut(s) 96
Tru1I TTAA 2 cut(s) 597, 648
Tru9I TTAA 2 cut(s) 597, 648
TscAI CASTG 3 cut(s) 385, 518, 777
TseFI GTSAC 2 cut(s) 848, 874
TseI GCWGC 4 cut(s) 668, 717, 862, 865
Tsp45I GTSAC 2 cut(s) 848, 874
TspDTI ATGAA 2 cut(s) 92, 714
TspGWI ACGGA 1 cut(s) 390
TspRI CASTG 3 cut(s) 385, 518, 777
Van91I CCANNNNNTGG 1 cut(s) 256
VpaK11BI GGWCC 4 cut(s) 139, 213, 437, 712
XapI RAATTY 1 cut(s) 196
XceI RCATGY 1 cut(s) 735
XmaJI CCTAGG 1 cut(s) 266
XmiI GTMKAC 1 cut(s) 317
XspI CTAG 2 cut(s) 111, 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.