FvH4_3g41220

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001338
Physical Location & Seq
Reverse (-)
34680742 .. 34683454
2713 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g41220.t1

Sequence Viewer

Length: 1557 bp
ATGGAGCTTCTTATTCCAATTGATGCAGACATGTTGATGTCTCTGGCTGTGATTGGAGTCGTATTGTTATTCCTACATCTTTATAATGCGCTTGTGGTGAGGCCGAAGAGGCTGCGATCCATGATTACCAAGCAAGGAATCAGTGGTCCGTCGCCGGCTTTTCTTCTGGGAAATATCATGGACATCAAGAAGGCCTATCGTCCGAGCAGCATTACTAAGGCTGCGACAAGCGAACCCTTAACTTACCACAACTGTGCTGCTGTTCTCTTCCCCTTCTTTGAGCAGTGGAGGAAGAAATATGGTGAAGTGTTTGTGTTTGCACTCGGCAACACGCAGATTCTATATGTGAACAAACCCGAGGCAGTGAAAGAGATTACAACATGCACATCCTGGGACTTGGGGAAGCCTAGTTATCAATACAAAGAGCGCCGTCCCTTACTTGGCCATGGTATTCTAACCTCCAATGGTGCCTCTTGGGCTCATCAGAGAAAAATCATTGCTCCTGAACTATACATGCAGAAAGTTAAGGGAATGATCAACTTAATCACAGAGTCTACAATGAGTCTGGTAGACTCTTGGAACAGTAAGATTGAGGCACAGGGAGGAATTGCAGACATAAAAATGGACCAGTGTATGAAAAGTTTCTCTGGTGATATTATCTCAAGGGCTTGTTTTGGAAGCAACTATCTAAAAGGCGAAGAGATATTTCAGAAACTAACAGATCTCCAGGAGGCCATGGCCAGGAAACTTTTCTTGACCGGAATACCTGGATTGAGACACCTTCCAACAAAGAGCAATAGGGAAGCTTGGGCATTAGAGAAGGAGGCTGCAACTTTGATACTACAGGTAGTGAAGGAGAGACAGACTGCAGGATATGAGAAAGACCTGTTGCAAATGATTCTGGAGGGTGCTAAAAACAGTGACCTTAGCCAAGAGGCAACAGACCGTTTCATTGTGGATAACTGCAAAAACATTTACTTGGCTGGATATGAAACCACAGCAGTTACTGCCACATGGTGCCTCATGTTGTTGGCTTCGAATCAAGAATGGCAAGAACGTGTCAGAGCCGAGGTACTTCAAGTTTGCGAGGGTCGAATTCCAGATGCTGATATGCTCCTTAAGATGAAACAGCTAACAATGGTAATTCATGAATCGTTGCGCCTTTATCCCCCAAACACTGTGGTGTCAAGGGAGGCTCTTAAGGACTTGAAATTTGGAGACATTCGTGTGCCAAAGGGTGTTAATATTTGGACAAATGTAGTAACCCTGCACACTGATCCTGAAATATGGGGACCGGATTCCTACACGTTCAAACCGGAGCGATTCGCCAATGGGATCTCTGGTGCCTGCAAACTTCCTTACTTGTACATGCCGTTTGGGATTGGACCACGAATATGTCTTGGACAGAATTTTGCAATGGTTGAACTCAAGATTCTGATAGCTCTCATACTCTCCAACTTCTCCTTCTCACTATCTCCCAAGTACATCCACAAGCCTTCTCTTAAGTTGGTTATTGAACCAGAACACGGAGTTGATCTCTTGGTGAAGAAGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

519

Amino Acids

58.33

Weight (kDa)

8.89

Isoelectric Point (pI)

38.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 91 - 502 4.7e-85 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 84
AccB1I GGYRCC 3 cut(s) 467, 1017, 1343
AccI GTMKAC 2 cut(s) 554, 570
AclWI GGATC 3 cut(s) 111, 1271, 1343
AcoI YGGCCR 2 cut(s) 442, 738
AcsI RAATTY 3 cut(s) 1095, 1211, 1408
AdeI CACNNNGTG 1 cut(s) 1017
AfaI GTAC 3 cut(s) 1074, 1367, 1484
AfiI CCNNNNNNNGG 3 cut(s) 440, 741, 1526
AflII CTTAAG 3 cut(s) 1118, 1199, 1502
AflIII ACRYGT 3 cut(s) 30, 1057, 1305
AgsI TTSAA 5 cut(s) 1079, 1210, 1312, 1424, 1517
AjnI CCWGG 4 cut(s) 389, 726, 740, 766
AjuI GAANNNNNNNTTGG 2 cut(s) 1448, 1480
AleI CACNNNNGTG 2 cut(s) 252, 1181
AluBI AGCT 5 cut(s) 7, 806, 1132, 1442, 1552
AluI AGCT 5 cut(s) 7, 806, 1132, 1442, 1552
Alw26I GTCTC 4 cut(s) 45, 769, 853, 1212
AlwI GGATC 3 cut(s) 111, 1271, 1343
AlwNI CAGNNNCTG 2 cut(s) 1007, 1106
Ama87I CYCGRG 1 cut(s) 356
AoxI GGCC 5 cut(s) 101, 192, 442, 732, 738
ApeKI GCWGC 5 cut(s) 112, 207, 221, 257, 827
ApoI RAATTY 3 cut(s) 1095, 1211, 1408
ArsI GACNNNNNNTTYG 2 cut(s) 1196, 1228
Asp700I GAANNNNTTC 2 cut(s) 641, 749
AspLEI GCGC 3 cut(s) 91, 429, 1161
AspS9I GGNCC 4 cut(s) 146, 625, 1292, 1385
AsuHPI GGTGA 4 cut(s) 109, 314, 662, 1555
AsuII TTCGAA 1 cut(s) 1037
AvaI CYCGRG 1 cut(s) 356
AvaII GGWCC 4 cut(s) 146, 625, 1292, 1385
BalI TGGCCA 2 cut(s) 444, 740
BanI GGYRCC 3 cut(s) 467, 1017, 1343
BanII GRGCYC 1 cut(s) 481
BbvI GCAGC 5 cut(s) 99, 208, 219, 244, 814
BceAI ACGGC 2 cut(s) 414, 1357
BcgI CGANNNNNNTGC 2 cut(s) 94, 128
BciT130I CCWGG 4 cut(s) 391, 728, 742, 768
BclI TGATCA 1 cut(s) 534
BcoDI GTCTC 4 cut(s) 45, 769, 853, 1212
BfaI CTAG 1 cut(s) 408
BfmI CTRYAG 2 cut(s) 842, 867
BfoI RGCGCY 1 cut(s) 430
BfrI CTTAAG 3 cut(s) 1118, 1199, 1502
BglI GCCNNNNNGGC 2 cut(s) 109, 476
BglII AGATCT 1 cut(s) 721
BisI GCNGC 5 cut(s) 113, 208, 222, 258, 828
BlsI GCNGC 5 cut(s) 114, 209, 223, 259, 829
Bme1390I CCNGG 4 cut(s) 391, 728, 742, 768
Bme18I GGWCC 4 cut(s) 146, 625, 1292, 1385
BmeT110I CYCGRG 1 cut(s) 356
BmgT120I GGNCC 4 cut(s) 146, 625, 1292, 1385
BmiI GGNNCC 4 cut(s) 469, 1019, 1293, 1345
BmrFI CCNGG 4 cut(s) 391, 728, 742, 768
BmsI GCATC 2 cut(s) 13, 1093
BplI GAGNNNNNCTC 2 cut(s) 1521, 1553
BpmI CTGGAG 2 cut(s) 710, 923
Bpu10I CCTNAGC 1 cut(s) 926
Bpu14I TTCGAA 1 cut(s) 1037
BpuEI CTTGAG 2 cut(s) 646, 1412
BsaJI CCNNGG 5 cut(s) 357, 390, 445, 735, 1068
BsaWI WCCGGW 3 cut(s) 758, 1294, 1315
Bsc4I CCNNNNNNNGG 3 cut(s) 440, 741, 1526
Bse118I RCCGGY 1 cut(s) 154
Bse1I ACTGG 1 cut(s) 628
Bse3DI GCAATG 2 cut(s) 495, 1422
BseBI CCWGG 4 cut(s) 391, 728, 742, 768
BseDI CCNNGG 5 cut(s) 357, 390, 445, 735, 1068
BseGI GGATG 2 cut(s) 386, 1485
BseLI CCNNNNNNNGG 3 cut(s) 440, 741, 1526
BseMI GCAATG 2 cut(s) 495, 1422
BseNI ACTGG 1 cut(s) 628
BseXI GCAGC 5 cut(s) 99, 208, 219, 244, 814
BsgI GTGCAG 1 cut(s) 1253
BshFI GGCC 5 cut(s) 103, 194, 444, 734, 740
BshNI GGYRCC 3 cut(s) 467, 1017, 1343
BsiHKCI CYCGRG 1 cut(s) 356
BsiSI CCGG 4 cut(s) 155, 759, 1295, 1316
BslFI GGGAC 3 cut(s) 407, 417, 1305
BslI CCNNNNNNNGG 3 cut(s) 440, 741, 1526
BsmAI GTCTC 4 cut(s) 45, 769, 853, 1212
BsmFI GGGAC 3 cut(s) 407, 417, 1305
BsnI GGCC 5 cut(s) 103, 194, 444, 734, 740
BsoBI CYCGRG 1 cut(s) 356
Bsp119I TTCGAA 1 cut(s) 1037
Bsp1286I GDGCHC 1 cut(s) 481
Bsp1407I TGTACA 1 cut(s) 1365
Bsp143I GATC 6 cut(s) 116, 534, 721, 1276, 1335, 1534
Bsp19I CCATGG 2 cut(s) 445, 735
BspANI GGCC 5 cut(s) 103, 194, 444, 734, 740
BspHI TCATGA 1 cut(s) 1147
BspLI GGNNCC 4 cut(s) 469, 1019, 1293, 1345
BspMAI CTGCAG 1 cut(s) 871
BspPI GGATC 3 cut(s) 111, 1271, 1343
BspT104I TTCGAA 1 cut(s) 1037
BspT107I GGYRCC 3 cut(s) 467, 1017, 1343
BspTI CTTAAG 3 cut(s) 1118, 1199, 1502
BsrDI GCAATG 2 cut(s) 495, 1422
BsrFI RCCGGY 1 cut(s) 154
BsrGI TGTACA 1 cut(s) 1365
BsrI ACTGG 1 cut(s) 628
BssAI RCCGGY 1 cut(s) 154
BssECI CCNNGG 5 cut(s) 357, 390, 445, 735, 1068
BssMI GATC 6 cut(s) 116, 534, 721, 1276, 1335, 1534
BssT1I CCWWGG 2 cut(s) 445, 735
Bst2UI CCWGG 4 cut(s) 391, 728, 742, 768
Bst4CI ACNGT 5 cut(s) 254, 584, 920, 947, 1180
Bst6I CTCTTC 3 cut(s) 101, 272, 693
BstAFI CTTAAG 3 cut(s) 1118, 1199, 1502
BstAPI GCANNNNNTGC 1 cut(s) 1007
BstAUI TGTACA 1 cut(s) 1365
BstBI TTCGAA 1 cut(s) 1037
BstC8I GCNNGC 2 cut(s) 156, 1348
BstDEI CTNAG 2 cut(s) 216, 926
BstDSI CCRYGG 2 cut(s) 445, 735
BstF5I GGATG 2 cut(s) 386, 1485
BstH2I RGCGCY 1 cut(s) 430
BstHHI GCGC 3 cut(s) 91, 429, 1161
BstKTI GATC 6 cut(s) 119, 537, 724, 1279, 1338, 1537
BstMAI GTCTC 4 cut(s) 45, 769, 853, 1212
BstMBI GATC 6 cut(s) 116, 534, 721, 1276, 1335, 1534
BstMWI GCNNNNNNNGC 3 cut(s) 109, 476, 1007
BstNI CCWGG 4 cut(s) 391, 728, 742, 768
BstNSI RCATGY 4 cut(s) 34, 384, 517, 1372
BstSCI CCNGG 4 cut(s) 389, 726, 740, 766
BstSFI CTRYAG 2 cut(s) 842, 867
BstV1I GCAGC 5 cut(s) 99, 208, 219, 244, 814
BstX2I RGATCY 2 cut(s) 721, 1335
BstYI RGATCY 2 cut(s) 721, 1335
BsuRI GGCC 5 cut(s) 103, 194, 444, 734, 740
BtgI CCRYGG 2 cut(s) 445, 735
BtsCI GGATG 2 cut(s) 386, 1485
BtsI GCAGTG 2 cut(s) 290, 369
BtsIMutI CAGTG 7 cut(s) 148, 290, 369, 635, 925, 1176, 1272
Cac8I GCNNGC 2 cut(s) 156, 1348
CaiI CAGNNNCTG 2 cut(s) 1007, 1106
CciI TCATGA 1 cut(s) 1147
CfoI GCGC 3 cut(s) 91, 429, 1161
Cfr10I RCCGGY 1 cut(s) 154
Cfr13I GGNCC 4 cut(s) 146, 625, 1292, 1385
Csp6I GTAC 3 cut(s) 1073, 1366, 1483
CspCI CAANNNNNGTGG 2 cut(s) 1161, 1196
CviQI GTAC 3 cut(s) 1073, 1366, 1483
DdeI CTNAG 2 cut(s) 216, 926
DpnI GATC 6 cut(s) 118, 536, 723, 1278, 1337, 1536
DpnII GATC 6 cut(s) 116, 534, 721, 1276, 1335, 1534
DraIII CACNNNGTG 1 cut(s) 1017
EaeI YGGCCR 2 cut(s) 442, 738
Eam1104I CTCTTC 3 cut(s) 101, 272, 693
EarI CTCTTC 3 cut(s) 101, 272, 693
Eco130I CCWWGG 2 cut(s) 445, 735
Eco147I AGGCCT 1 cut(s) 194
Eco24I GRGCYC 1 cut(s) 481
Eco47I GGWCC 4 cut(s) 146, 625, 1292, 1385
Eco88I CYCGRG 1 cut(s) 356
EcoRI GAATTC 1 cut(s) 1095
EcoRII CCWGG 4 cut(s) 389, 726, 740, 766
EcoT14I CCWWGG 2 cut(s) 445, 735
EcoT38I GRGCYC 1 cut(s) 481
ErhI CCWWGG 2 cut(s) 445, 735
FaqI GGGAC 3 cut(s) 407, 417, 1305
FbaI TGATCA 1 cut(s) 534
FblI GTMKAC 2 cut(s) 554, 570
Fnu4HI GCNGC 5 cut(s) 113, 208, 222, 258, 828
FokI GGATG 2 cut(s) 373, 1472
FriOI GRGCYC 1 cut(s) 481
Fsp4HI GCNGC 5 cut(s) 113, 208, 222, 258, 828
FspBI CTAG 1 cut(s) 408
GlaI GCGC 3 cut(s) 90, 428, 1160
GluI GCNGC 5 cut(s) 113, 208, 222, 258, 828
GsuI CTGGAG 2 cut(s) 710, 923
HaeII RGCGCY 1 cut(s) 430
HaeIII GGCC 5 cut(s) 103, 194, 444, 734, 740
HapII CCGG 4 cut(s) 155, 759, 1295, 1316
HhaI GCGC 3 cut(s) 91, 429, 1161
Hin6I GCGC 3 cut(s) 89, 427, 1159
HinP1I GCGC 3 cut(s) 89, 427, 1159
HindIII AAGCTT 1 cut(s) 804
HpaII CCGG 4 cut(s) 155, 759, 1295, 1316
HphI GGTGA 4 cut(s) 109, 314, 662, 1555
Hpy166II GTNNAC 3 cut(s) 349, 555, 571
Hpy188I TCNGA 5 cut(s) 204, 486, 711, 1064, 1437
Hpy188III TCNNGA 9 cut(s) 187, 503, 754, 902, 1043, 1100, 1148, 1280, 1429
Hpy8I GTNNAC 3 cut(s) 349, 555, 571
Hpy99I CGWCG 1 cut(s) 154
HpyAV CCTTC 7 cut(s) 184, 283, 791, 814, 847, 1474, 1506
HpyCH4III ACNGT 5 cut(s) 254, 584, 920, 947, 1180
HpyCH4IV ACGT 2 cut(s) 1057, 1307
HpyF10VI GCNNNNNNNGC 3 cut(s) 109, 476, 1007
HpyF3I CTNAG 2 cut(s) 216, 926
HpySE526I ACGT 2 cut(s) 1057, 1307
HspAI GCGC 3 cut(s) 89, 427, 1159
KroI GCCGGC 1 cut(s) 154
KroNI GCCGGC 1 cut(s) 156
Ksp22I TGATCA 1 cut(s) 534
Kzo9I GATC 6 cut(s) 116, 534, 721, 1276, 1335, 1534
LmnI GCTCC 4 cut(s) 4, 505, 1119, 1318
Lsp1109I GCAGC 5 cut(s) 99, 208, 219, 244, 814
LweI GCATC 2 cut(s) 13, 1093
MaeI CTAG 1 cut(s) 408
MaeII ACGT 2 cut(s) 1057, 1307
MaeIII GTNAC 3 cut(s) 920, 1003, 1261
MalI GATC 6 cut(s) 118, 536, 723, 1278, 1337, 1536
MboI GATC 6 cut(s) 116, 534, 721, 1276, 1335, 1534
MboII GAAGA 5 cut(s) 118, 155, 259, 304, 710
MfeI CAATTG 1 cut(s) 18
MflI RGATCY 2 cut(s) 721, 1335
MhlI GDGCHC 1 cut(s) 481
MlsI TGGCCA 2 cut(s) 444, 740
MluCI AATT 6 cut(s) 18, 606, 1095, 1143, 1211, 1408
MluNI TGGCCA 2 cut(s) 444, 740
MlyI GAGTC 4 cut(s) 66, 560, 566, 571
MmeI TCCRAC 2 cut(s) 809, 1479
Mox20I TGGCCA 2 cut(s) 444, 740
MroNI GCCGGC 1 cut(s) 154
MroXI GAANNNNTTC 2 cut(s) 641, 749
MscI TGGCCA 2 cut(s) 444, 740
MseI TTAA 7 cut(s) 239, 525, 542, 1119, 1200, 1242, 1503
MslI CAYNNNNRTG 6 cut(s) 35, 252, 620, 1181, 1226, 1393
Msp20I TGGCCA 2 cut(s) 444, 740
MspCI CTTAAG 3 cut(s) 1118, 1199, 1502
MspI CCGG 4 cut(s) 155, 759, 1295, 1316
MspR9I CCNGG 4 cut(s) 391, 728, 742, 768
MunI CAATTG 1 cut(s) 18
MvaI CCWGG 4 cut(s) 391, 728, 742, 768
MwoI GCNNNNNNNGC 3 cut(s) 109, 476, 1007
NaeI GCCGGC 1 cut(s) 156
NcoI CCATGG 2 cut(s) 445, 735
NdeII GATC 6 cut(s) 116, 534, 721, 1276, 1335, 1534
NgoMIV GCCGGC 1 cut(s) 154
NlaIV GGNNCC 4 cut(s) 469, 1019, 1293, 1345
NmeAIII GCCGAG 2 cut(s) 303, 1093
NmuCI GTSAC 1 cut(s) 920
NspI RCATGY 4 cut(s) 34, 384, 517, 1372
NspV TTCGAA 1 cut(s) 1037
OliI CACNNNNGTG 2 cut(s) 252, 1181
PagI TCATGA 1 cut(s) 1147
PceI AGGCCT 1 cut(s) 194
PciI ACATGT 1 cut(s) 30
PdiI GCCGGC 1 cut(s) 156
PdmI GAANNNNTTC 2 cut(s) 641, 749
PfeI GAWTC 8 cut(s) 138, 337, 898, 1039, 1151, 1298, 1323, 1432
PfoI TCCNGGA 1 cut(s) 726
PkrI GCNGC 5 cut(s) 114, 209, 223, 259, 829
PleI GAGTC 4 cut(s) 65, 559, 566, 570
PpsI GAGTC 4 cut(s) 65, 559, 566, 570
PscI ACATGT 1 cut(s) 30
PsiI TTATAA 1 cut(s) 84
Psp6I CCWGG 4 cut(s) 389, 726, 740, 766
PspGI CCWGG 4 cut(s) 389, 726, 740, 766
PspN4I GGNNCC 4 cut(s) 469, 1019, 1293, 1345
PspPI GGNCC 4 cut(s) 146, 625, 1292, 1385
PstI CTGCAG 1 cut(s) 871
PstNI CAGNNNCTG 2 cut(s) 1007, 1106
PsuI RGATCY 2 cut(s) 721, 1335
RsaI GTAC 3 cut(s) 1074, 1367, 1484
RsaNI GTAC 3 cut(s) 1073, 1366, 1483
RseI CAYNNNNRTG 6 cut(s) 35, 252, 620, 1181, 1226, 1393
SaqAI TTAA 7 cut(s) 239, 525, 542, 1119, 1200, 1242, 1503
SatI GCNGC 5 cut(s) 113, 208, 222, 258, 828
Sau3AI GATC 6 cut(s) 116, 534, 721, 1276, 1335, 1534
Sau96I GGNCC 4 cut(s) 146, 625, 1292, 1385
SchI GAGTC 4 cut(s) 66, 560, 566, 571
ScrFI CCNGG 4 cut(s) 391, 728, 742, 768
SduI GDGCHC 1 cut(s) 481
SfaNI GCATC 2 cut(s) 13, 1093
SfcI CTRYAG 2 cut(s) 842, 867
SfuI TTCGAA 1 cut(s) 1037
SinI GGWCC 4 cut(s) 146, 625, 1292, 1385
SmiMI CAYNNNNRTG 6 cut(s) 35, 252, 620, 1181, 1226, 1393
SmlI CTYRAG 5 cut(s) 661, 1118, 1199, 1427, 1502
SmoI CTYRAG 5 cut(s) 661, 1118, 1199, 1427, 1502
Sse9I AATT 6 cut(s) 18, 606, 1095, 1143, 1211, 1408
SseBI AGGCCT 1 cut(s) 194
SspI AATATT 1 cut(s) 1246
SspMI CTAG 1 cut(s) 408
StuI AGGCCT 1 cut(s) 194
StyD4I CCNGG 4 cut(s) 389, 726, 740, 766
StyI CCWWGG 2 cut(s) 445, 735
TaaI ACNGT 5 cut(s) 254, 584, 920, 947, 1180
TaiI ACGT 2 cut(s) 1060, 1310
TaqI TCGA 2 cut(s) 1037, 1093
TasI AATT 6 cut(s) 18, 606, 1095, 1143, 1211, 1408
TatI WGTACW 2 cut(s) 1365, 1482
TfiI GAWTC 8 cut(s) 138, 337, 898, 1039, 1151, 1298, 1323, 1432
Tru1I TTAA 7 cut(s) 239, 525, 542, 1119, 1200, 1242, 1503
Tru9I TTAA 7 cut(s) 239, 525, 542, 1119, 1200, 1242, 1503
TscAI CASTG 7 cut(s) 148, 290, 369, 635, 925, 1183, 1279
TseFI GTSAC 1 cut(s) 920
TseI GCWGC 5 cut(s) 112, 207, 221, 257, 827
Tsp45I GTSAC 1 cut(s) 920
TspDTI ATGAA 6 cut(s) 650, 940, 1005, 1136, 1139, 1164
TspGWI ACGGA 2 cut(s) 138, 1542
TspRI CASTG 7 cut(s) 148, 290, 369, 635, 925, 1183, 1279
Vha464I CTTAAG 3 cut(s) 1118, 1199, 1502
VpaK11BI GGWCC 4 cut(s) 146, 625, 1292, 1385
XapI RAATTY 3 cut(s) 1095, 1211, 1408
XceI RCATGY 4 cut(s) 34, 384, 517, 1372
XmiI GTMKAC 2 cut(s) 554, 570
XmnI GAANNNNTTC 2 cut(s) 641, 749
XspI CTAG 1 cut(s) 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.