Rh5AG485200

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0001338
Physical Location & Seq
Forward (+)
82954816 .. 82957398
2583 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG485200.1

Sequence Viewer

Length: 1551 bp
ATGGAGCTTCTTCACTTTGATGCAAAAATGTTAATGTCTCTGGCTATGCTTGGAGTCATAGGGTTATTAGTACGTCTGTATAATGGGCTTGTGGTGAAGCCGAAGAGGCTGCGGTCCTTGATAACCAAGCAAGGCATCAGCGGTCCCTCGCCGGCTTTTCTCCTGGGAAATATCATGGACATTAAGAAGGCTCGTCCGAGCAGCATTACTAAGGCTCCGACAAGCGAACCCCCCACCTCCCACAACTGTGCTGCTGTTCTCTTCCCCTTTTTTGAGCAGTGGAGGAAGAAATATGGTGAAGTGTTTGTGTTTGCACTCGGCAACACACAGGTACTATATGTGAACAAACCTGAGGTAGTGAAGGAGATTACCACATGCACATCCTGGGACTTGGGGAAGCCTAGTTATCAGTATAAAGAGCGCCGTCCCTTACTTGGTCATGGTATTCTAACCTCGAACGGTGCCTCATGGGCTCATCAGAGAAAAATCATTGCTCCTGAACTATACATGCAGAAAGTTAAGGGAATGATCAAATTAATCACAGAGTCTACAATTGCTCTGGTAAATTCATGGAACAGTAAGATTGAGGCACAGGGAGGAATTGCAGACATAAAAATGGACCACTATATGAGAAGTTTCTCTGGTGATGTTATCTCAAGGGCGTGTTTCGGAAGCAACTATTTGAAAGGAGAAGAGATATTTCAGAAACTAACCGATCTCCAAGAGGCCATGGCCAGGAAACTTTTCTTGACCGGAATACCTGGATTGAGACACCTTCCAACAAAGAGTAATAGGGAAGCTTGGGCATTAGAGAAGGAGGCTGCAACTTTGATACTACGGGTAGTGAAGGAGAGACAGGCTGCGGGGTACGAGAAAGACCTATTGCAAATGATTCTTGAGGGGGCTAAAAACAGTGACCTTAGCCAGGAGGCAACAGACTGTTTCATTGTCGATAACTGCAAAAACATTTACTTGGCTGGATATGAAACCACAGCAGTTTCTGCCACATGGTGCCTCATGTTGTTGGCTTTGAATCAAGAATGGCAAGAACGTGTCAGAGCGGAGGTTCTTCAAGTCTTCGAGGGCCGAATTCCAGATGCTGATATGCTCCTTAAGATGAAACAGCTAACAATGGTGATTCATGAATCGTTGCGCCTTTATCCTCCAAACACTGTGGTGTCAAGGGAGGCTCTCAAGGACTTGAAATTTGGAGACATTCATGTGCCAAAGGGTGTCAATGTTTGGACAGATGTAGTAACCCTACACACCGATCCTGAAATATGGGGACCGGATTCCTACACATTCAACCCCGAGCGATTTTCCAATGGGATCTCTGGTGCCTGCAAGCTTCCCTACTTGTACATGCCGTTTGGGATTGGACCACGAATATGTCTTGGACAGAACTTTGCAATGGTTGAACTCAAGATTCTGATAGCTCTCATACTCTCCAACTTCTCCTTCTCACTATCTCCCAAGTACATCCACAAGCCTTCTCTTAAGTTGGTTATTGAACCAGAACACGGAGTTGATCTCTTGGTAAAAAAGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

516

Amino Acids

58.02

Weight (kDa)

9.06

Isoelectric Point (pI)

39.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 89 - 500 7.4e-84 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 461, 1011, 1337
AccBSI CCGCTC 1 cut(s) 1061
AccI GTMKAC 1 cut(s) 548
AciI CCGC 4 cut(s) 112, 141, 863, 1061
AclWI GGATC 2 cut(s) 1265, 1337
AcoI YGGCCR 1 cut(s) 732
AcsI RAATTY 3 cut(s) 565, 1089, 1205
AdeI CACNNNGTG 1 cut(s) 1011
AfaI GTAC 5 cut(s) 72, 333, 869, 1361, 1478
AfiI CCNNNNNNNGG 4 cut(s) 434, 735, 925, 1520
AflII CTTAAG 2 cut(s) 1112, 1496
AflIII ACRYGT 1 cut(s) 1051
AgsI TTSAA 7 cut(s) 685, 1033, 1073, 1204, 1306, 1418, 1511
AjnI CCWGG 5 cut(s) 162, 383, 734, 760, 924
AjuI GAANNNNNNNTTGG 2 cut(s) 1442, 1474
AleI CACNNNNGTG 2 cut(s) 246, 1175
AluBI AGCT 6 cut(s) 7, 800, 1126, 1348, 1436, 1546
AluI AGCT 6 cut(s) 7, 800, 1126, 1348, 1436, 1546
Alw26I GTCTC 4 cut(s) 42, 763, 847, 1206
AlwI GGATC 2 cut(s) 1265, 1337
AlwNI CAGNNNCTG 2 cut(s) 1001, 1100
Ama87I CYCGRG 1 cut(s) 1310
AoxI GGCC 3 cut(s) 726, 732, 1084
ApeKI GCWGC 5 cut(s) 109, 201, 251, 821, 860
ApoI RAATTY 3 cut(s) 565, 1089, 1205
ArsI GACNNNNNNTTYG 2 cut(s) 1190, 1222
AseI ATTAAT 1 cut(s) 536
Asp700I GAANNNNTTC 1 cut(s) 743
AspLEI GCGC 2 cut(s) 423, 1155
AspS9I GGNCC 6 cut(s) 114, 143, 619, 1084, 1286, 1379
AsuHPI GGTGA 4 cut(s) 106, 308, 656, 1147
AvaI CYCGRG 1 cut(s) 1310
AvaII GGWCC 5 cut(s) 114, 143, 619, 1286, 1379
AxyI CCTNAGG 1 cut(s) 351
BalI TGGCCA 1 cut(s) 734
BanI GGYRCC 3 cut(s) 461, 1011, 1337
BanII GRGCYC 1 cut(s) 475
BbsI GAAGAC 1 cut(s) 1069
BbvI GCAGC 5 cut(s) 96, 213, 238, 808, 847
BceAI ACGGC 2 cut(s) 408, 1351
BcgI CGANNNNNNTGC 2 cut(s) 91, 125
BciT130I CCWGG 5 cut(s) 164, 385, 736, 762, 926
BclI TGATCA 1 cut(s) 528
BcoDI GTCTC 4 cut(s) 42, 763, 847, 1206
BfaI CTAG 1 cut(s) 402
BfoI RGCGCY 1 cut(s) 424
BfrI CTTAAG 2 cut(s) 1112, 1496
BglI GCCNNNNNGGC 2 cut(s) 106, 470
BisI GCNGC 5 cut(s) 110, 202, 252, 822, 861
BlsI GCNGC 5 cut(s) 111, 203, 253, 823, 862
Bme1390I CCNGG 5 cut(s) 164, 385, 736, 762, 926
Bme18I GGWCC 5 cut(s) 114, 143, 619, 1286, 1379
BmeT110I CYCGRG 1 cut(s) 1310
BmgT120I GGNCC 6 cut(s) 114, 143, 619, 1084, 1286, 1379
BmiI GGNNCC 6 cut(s) 145, 216, 463, 1013, 1287, 1339
BmrFI CCNGG 5 cut(s) 164, 385, 736, 762, 926
BmsI GCATC 3 cut(s) 10, 144, 1087
BpiI GAAGAC 1 cut(s) 1069
BplI GAGNNNNNCTC 2 cut(s) 1515, 1547
Bpu10I CCTNAGC 1 cut(s) 920
BpuEI CTTGAG 4 cut(s) 640, 917, 1178, 1406
BsaJI CCNNGG 3 cut(s) 163, 384, 729
BsaWI WCCGGW 2 cut(s) 752, 1288
BsaXI ACNNNNNCTCC 2 cut(s) 199, 229
Bsc4I CCNNNNNNNGG 4 cut(s) 434, 735, 925, 1520
Bse118I RCCGGY 1 cut(s) 151
Bse21I CCTNAGG 1 cut(s) 351
Bse3DI GCAATG 2 cut(s) 489, 1416
BseBI CCWGG 5 cut(s) 164, 385, 736, 762, 926
BseDI CCNNGG 3 cut(s) 163, 384, 729
BseGI GGATG 2 cut(s) 380, 1479
BseLI CCNNNNNNNGG 4 cut(s) 434, 735, 925, 1520
BseMI GCAATG 2 cut(s) 489, 1416
BseMII CTCAG 1 cut(s) 342
BseXI GCAGC 5 cut(s) 96, 213, 238, 808, 847
BshFI GGCC 3 cut(s) 728, 734, 1086
BshNI GGYRCC 3 cut(s) 461, 1011, 1337
BsiHKCI CYCGRG 1 cut(s) 1310
BsiSI CCGG 3 cut(s) 152, 753, 1289
BslFI GGGAC 4 cut(s) 129, 401, 411, 1299
BslI CCNNNNNNNGG 4 cut(s) 434, 735, 925, 1520
BsmAI GTCTC 4 cut(s) 42, 763, 847, 1206
BsmFI GGGAC 4 cut(s) 129, 401, 411, 1299
BsnI GGCC 3 cut(s) 728, 734, 1086
BsoBI CYCGRG 1 cut(s) 1310
Bsp1286I GDGCHC 1 cut(s) 475
Bsp1407I TGTACA 1 cut(s) 1359
Bsp143I GATC 5 cut(s) 528, 715, 1270, 1329, 1528
Bsp19I CCATGG 1 cut(s) 729
BspACI CCGC 4 cut(s) 112, 141, 863, 1061
BspANI GGCC 3 cut(s) 728, 734, 1086
BspCNI CTCAG 1 cut(s) 343
BspHI TCATGA 1 cut(s) 1141
BspLI GGNNCC 6 cut(s) 145, 216, 463, 1013, 1287, 1339
BspPI GGATC 2 cut(s) 1265, 1337
BspT107I GGYRCC 3 cut(s) 461, 1011, 1337
BspTI CTTAAG 2 cut(s) 1112, 1496
BsrBI CCGCTC 1 cut(s) 1061
BsrDI GCAATG 2 cut(s) 489, 1416
BsrFI RCCGGY 1 cut(s) 151
BsrGI TGTACA 1 cut(s) 1359
BssAI RCCGGY 1 cut(s) 151
BssECI CCNNGG 3 cut(s) 163, 384, 729
BssMI GATC 5 cut(s) 528, 715, 1270, 1329, 1528
BssT1I CCWWGG 1 cut(s) 729
Bst2UI CCWGG 5 cut(s) 164, 385, 736, 762, 926
Bst4CI ACNGT 6 cut(s) 248, 461, 578, 914, 941, 1174
Bst6I CTCTTC 3 cut(s) 98, 266, 687
BstAFI CTTAAG 2 cut(s) 1112, 1496
BstAPI GCANNNNNTGC 1 cut(s) 1001
BstAUI TGTACA 1 cut(s) 1359
BstC8I GCNNGC 3 cut(s) 153, 1342, 1346
BstDEI CTNAG 3 cut(s) 210, 351, 920
BstDSI CCRYGG 1 cut(s) 729
BstENI CCTNNNNNAGG 1 cut(s) 923
BstF5I GGATG 2 cut(s) 380, 1479
BstH2I RGCGCY 1 cut(s) 424
BstHHI GCGC 2 cut(s) 423, 1155
BstKTI GATC 5 cut(s) 531, 718, 1273, 1332, 1531
BstMAI GTCTC 4 cut(s) 42, 763, 847, 1206
BstMBI GATC 5 cut(s) 528, 715, 1270, 1329, 1528
BstMWI GCNNNNNNNGC 3 cut(s) 106, 470, 1001
BstNI CCWGG 5 cut(s) 164, 385, 736, 762, 926
BstNSI RCATGY 3 cut(s) 378, 511, 1366
BstSCI CCNGG 5 cut(s) 162, 383, 734, 760, 924
BstV1I GCAGC 5 cut(s) 96, 213, 238, 808, 847
BstV2I GAAGAC 1 cut(s) 1069
BstX2I RGATCY 1 cut(s) 1329
BstYI RGATCY 1 cut(s) 1329
Bsu36I CCTNAGG 1 cut(s) 351
BsuRI GGCC 3 cut(s) 728, 734, 1086
BtgI CCRYGG 1 cut(s) 729
BtsCI GGATG 2 cut(s) 380, 1479
BtsI GCAGTG 1 cut(s) 284
BtsIMutI CAGTG 3 cut(s) 284, 919, 1170
Cac8I GCNNGC 3 cut(s) 153, 1342, 1346
CaiI CAGNNNCTG 2 cut(s) 1001, 1100
CciI TCATGA 1 cut(s) 1141
CfoI GCGC 2 cut(s) 423, 1155
Cfr10I RCCGGY 1 cut(s) 151
Cfr13I GGNCC 6 cut(s) 114, 143, 619, 1084, 1286, 1379
Csp6I GTAC 5 cut(s) 71, 332, 868, 1360, 1477
CspCI CAANNNNNGTGG 2 cut(s) 1155, 1190
CviQI GTAC 5 cut(s) 71, 332, 868, 1360, 1477
DdeI CTNAG 3 cut(s) 210, 351, 920
DpnI GATC 5 cut(s) 530, 717, 1272, 1331, 1530
DpnII GATC 5 cut(s) 528, 715, 1270, 1329, 1528
DraIII CACNNNGTG 1 cut(s) 1011
EaeI YGGCCR 1 cut(s) 732
Eam1104I CTCTTC 3 cut(s) 98, 266, 687
EarI CTCTTC 3 cut(s) 98, 266, 687
Eco130I CCWWGG 1 cut(s) 729
Eco24I GRGCYC 1 cut(s) 475
Eco47I GGWCC 5 cut(s) 114, 143, 619, 1286, 1379
Eco81I CCTNAGG 1 cut(s) 351
Eco88I CYCGRG 1 cut(s) 1310
EcoNI CCTNNNNNAGG 1 cut(s) 923
EcoRI GAATTC 1 cut(s) 1089
EcoRII CCWGG 5 cut(s) 162, 383, 734, 760, 924
EcoT14I CCWWGG 1 cut(s) 729
EcoT38I GRGCYC 1 cut(s) 475
ErhI CCWWGG 1 cut(s) 729
FaqI GGGAC 4 cut(s) 129, 401, 411, 1299
FauI CCCGC 1 cut(s) 856
FbaI TGATCA 1 cut(s) 528
FblI GTMKAC 1 cut(s) 548
Fnu4HI GCNGC 5 cut(s) 110, 202, 252, 822, 861
FokI GGATG 2 cut(s) 367, 1466
FriOI GRGCYC 1 cut(s) 475
Fsp4HI GCNGC 5 cut(s) 110, 202, 252, 822, 861
FspBI CTAG 1 cut(s) 402
GlaI GCGC 2 cut(s) 422, 1154
GluI GCNGC 5 cut(s) 110, 202, 252, 822, 861
HaeII RGCGCY 1 cut(s) 424
HaeIII GGCC 3 cut(s) 728, 734, 1086
HapII CCGG 3 cut(s) 152, 753, 1289
HhaI GCGC 2 cut(s) 423, 1155
Hin6I GCGC 2 cut(s) 421, 1153
HinP1I GCGC 2 cut(s) 421, 1153
HindIII AAGCTT 2 cut(s) 798, 1346
HinfI GANTC 8 cut(s) 54, 545, 892, 1033, 1138, 1145, 1292, 1426
HpaII CCGG 3 cut(s) 152, 753, 1289
HphI GGTGA 4 cut(s) 106, 308, 656, 1147
Hpy166II GTNNAC 2 cut(s) 343, 549
Hpy188I TCNGA 7 cut(s) 198, 219, 480, 671, 705, 1058, 1431
Hpy188III TCNNGA 8 cut(s) 497, 748, 896, 1037, 1094, 1142, 1274, 1423
Hpy8I GTNNAC 2 cut(s) 343, 549
HpyAV CCTTC 7 cut(s) 181, 355, 785, 808, 841, 1468, 1500
HpyCH4III ACNGT 6 cut(s) 248, 461, 578, 914, 941, 1174
HpyCH4IV ACGT 2 cut(s) 73, 1051
HpyF10VI GCNNNNNNNGC 3 cut(s) 106, 470, 1001
HpyF3I CTNAG 3 cut(s) 210, 351, 920
HpySE526I ACGT 2 cut(s) 73, 1051
HspAI GCGC 2 cut(s) 421, 1153
KroI GCCGGC 1 cut(s) 151
KroNI GCCGGC 1 cut(s) 153
Ksp22I TGATCA 1 cut(s) 528
Kzo9I GATC 5 cut(s) 528, 715, 1270, 1329, 1528
LmnI GCTCC 4 cut(s) 4, 220, 499, 1113
Lsp1109I GCAGC 5 cut(s) 96, 213, 238, 808, 847
LweI GCATC 3 cut(s) 10, 144, 1087
MaeI CTAG 1 cut(s) 402
MaeII ACGT 2 cut(s) 73, 1051
MaeIII GTNAC 2 cut(s) 914, 1255
MalI GATC 5 cut(s) 530, 717, 1272, 1331, 1530
MbiI CCGCTC 1 cut(s) 1061
MboI GATC 5 cut(s) 528, 715, 1270, 1329, 1528
MboII GAAGA 6 cut(s) 115, 253, 298, 704, 1061, 1069
MfeI CAATTG 1 cut(s) 552
MflI RGATCY 1 cut(s) 1329
MhlI GDGCHC 1 cut(s) 475
MlsI TGGCCA 1 cut(s) 734
MluCI AATT 6 cut(s) 533, 552, 565, 600, 1089, 1205
MluNI TGGCCA 1 cut(s) 734
MlyI GAGTC 2 cut(s) 63, 554
MmeI TCCRAC 3 cut(s) 242, 803, 1473
Mox20I TGGCCA 1 cut(s) 734
MroNI GCCGGC 1 cut(s) 151
MroXI GAANNNNTTC 1 cut(s) 743
MscI TGGCCA 1 cut(s) 734
MseI TTAA 6 cut(s) 32, 183, 519, 536, 1113, 1497
MslI CAYNNNNRTG 6 cut(s) 18, 246, 614, 1175, 1220, 1387
Msp20I TGGCCA 1 cut(s) 734
MspA1I CMGCKG 1 cut(s) 141
MspCI CTTAAG 2 cut(s) 1112, 1496
MspI CCGG 3 cut(s) 152, 753, 1289
MspR9I CCNGG 5 cut(s) 164, 385, 736, 762, 926
MunI CAATTG 1 cut(s) 552
MvaI CCWGG 5 cut(s) 164, 385, 736, 762, 926
MwoI GCNNNNNNNGC 3 cut(s) 106, 470, 1001
NaeI GCCGGC 1 cut(s) 153
NcoI CCATGG 1 cut(s) 729
NdeII GATC 5 cut(s) 528, 715, 1270, 1329, 1528
NgoMIV GCCGGC 1 cut(s) 151
NlaIV GGNNCC 6 cut(s) 145, 216, 463, 1013, 1287, 1339
NmeAIII GCCGAG 1 cut(s) 297
NmuCI GTSAC 1 cut(s) 914
NspI RCATGY 3 cut(s) 378, 511, 1366
OliI CACNNNNGTG 2 cut(s) 246, 1175
PagI TCATGA 1 cut(s) 1141
PdiI GCCGGC 1 cut(s) 153
PdmI GAANNNNTTC 1 cut(s) 743
PfeI GAWTC 6 cut(s) 892, 1033, 1138, 1145, 1292, 1426
PkrI GCNGC 5 cut(s) 111, 203, 253, 823, 862
PleI GAGTC 2 cut(s) 62, 553
PpsI GAGTC 2 cut(s) 62, 553
PshBI ATTAAT 1 cut(s) 536
Psp6I CCWGG 5 cut(s) 162, 383, 734, 760, 924
PspGI CCWGG 5 cut(s) 162, 383, 734, 760, 924
PspN4I GGNNCC 6 cut(s) 145, 216, 463, 1013, 1287, 1339
PspPI GGNCC 6 cut(s) 114, 143, 619, 1084, 1286, 1379
PstNI CAGNNNCTG 2 cut(s) 1001, 1100
PsuI RGATCY 1 cut(s) 1329
RsaI GTAC 5 cut(s) 72, 333, 869, 1361, 1478
RsaNI GTAC 5 cut(s) 71, 332, 868, 1360, 1477
RseI CAYNNNNRTG 6 cut(s) 18, 246, 614, 1175, 1220, 1387
SaqAI TTAA 6 cut(s) 32, 183, 519, 536, 1113, 1497
SatI GCNGC 5 cut(s) 110, 202, 252, 822, 861
Sau3AI GATC 5 cut(s) 528, 715, 1270, 1329, 1528
Sau96I GGNCC 6 cut(s) 114, 143, 619, 1084, 1286, 1379
SchI GAGTC 2 cut(s) 63, 554
ScrFI CCNGG 5 cut(s) 164, 385, 736, 762, 926
SduI GDGCHC 1 cut(s) 475
SfaNI GCATC 3 cut(s) 10, 144, 1087
SinI GGWCC 5 cut(s) 114, 143, 619, 1286, 1379
SmiMI CAYNNNNRTG 6 cut(s) 18, 246, 614, 1175, 1220, 1387
SmlI CTYRAG 6 cut(s) 655, 896, 1112, 1193, 1421, 1496
SmoI CTYRAG 6 cut(s) 655, 896, 1112, 1193, 1421, 1496
Sse9I AATT 6 cut(s) 533, 552, 565, 600, 1089, 1205
SsiI CCGC 4 cut(s) 112, 141, 863, 1061
SspMI CTAG 1 cut(s) 402
StyD4I CCNGG 5 cut(s) 162, 383, 734, 760, 924
StyI CCWWGG 1 cut(s) 729
TaaI ACNGT 6 cut(s) 248, 461, 578, 914, 941, 1174
TaiI ACGT 2 cut(s) 76, 1054
TaqI TCGA 3 cut(s) 455, 951, 1080
TasI AATT 6 cut(s) 533, 552, 565, 600, 1089, 1205
TatI WGTACW 2 cut(s) 1359, 1476
TfiI GAWTC 6 cut(s) 892, 1033, 1138, 1145, 1292, 1426
Tru1I TTAA 6 cut(s) 32, 183, 519, 536, 1113, 1497
Tru9I TTAA 6 cut(s) 32, 183, 519, 536, 1113, 1497
TscAI CASTG 3 cut(s) 284, 919, 1177
TseFI GTSAC 1 cut(s) 914
TseI GCWGC 5 cut(s) 109, 201, 251, 821, 860
Tsp45I GTSAC 1 cut(s) 914
TspDTI ATGAA 7 cut(s) 558, 934, 999, 1130, 1133, 1158, 1208
TspGWI ACGGA 1 cut(s) 1536
TspRI CASTG 3 cut(s) 284, 919, 1177
Vha464I CTTAAG 2 cut(s) 1112, 1496
VpaK11BI GGWCC 5 cut(s) 114, 143, 619, 1286, 1379
VspI ATTAAT 1 cut(s) 536
XagI CCTNNNNNAGG 1 cut(s) 923
XapI RAATTY 3 cut(s) 565, 1089, 1205
XceI RCATGY 3 cut(s) 378, 511, 1366
XmiI GTMKAC 1 cut(s) 548
XmnI GAANNNNTTC 1 cut(s) 743
XspI CTAG 1 cut(s) 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.