MD03G1046800.v1.1

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0001338
Physical Location & Seq
Forward (+)
3763079 .. 3765620
2542 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1046800.v1.1.491

Sequence Viewer

Length: 1479 bp
ATGCAGCTCCTCCAATTTGATGCAAAAGTGTTACTGTCCCTTGTGTTGCTAGGGTTTATAGGATTGGTGGCACGTCTCCACAACGGGCTTGTTGCGAAGCCAAAGAGGCTTCGATCCTTGCTAACCAAGCAAGGAATCAATGGACCTCCACCTACTATTCTCCTGGGAAATATTTTGGAAATAAATTTATGTCTGCTAAAGGGTAGTGGAAAATTTTCTACAGGTGAAGTATTTGTGTTTGCACTTGGAAACACACAGATATTATGTGTGCACCAACCTGATGCAGTGAGGGACATTACAACATGCACATCCTTGGACTTGGGGAAACCAACGTATCAATTTAAAGAGCGCGGTCCTTTGCTTGGTCAAGGTATTTTAACCTCAAATGGCTCCTCGTGGGTGCATCAGCGCAAAATCATTTCTCCTGAACTATACATGGAGAAAGTTAAGGGAATGATTAACTTAATTACGGAGTCTACAATTACTCTGATAAATTCGTGGAACAGTAGAAACGAGGTAGAGGGAGGAATTTCTGACATAAAAATAGATAGCTACATGGGAAGTTTCTCTGGCGATGCTATCTCAAGAGCTTGTTTTGGAAGCAACTATTCCACAGGGGAGGAGATATTTCAGAAACTAAAGCAACTCCAGGAGGCTATGGGCAGGAAAGCTTTTCTGGTCGGAATTCCTGGAATGAGGCACCTTCCCACAAAGAGCAATAGTGAAGTTTGGACCTTAGAAAAGGAGGCTAACTCTTTGATACTGCAGGTAGTGAAGGAAAGACGGGCAGCAGAATATGAGAAAGACTTATTGCAAATTATTCTTGGGGGTGCCAAAAACAGTAACCTTAGCCAAGAGGCAACAGACCGCTTTATCGTTGATAACTGCAAGAACATAAACTTGGCCGGGTATGAAACCACAGCAGTTTCAGCCACATGGTGCCTCATGCTGTTGGCTTCGAACCAAGAATGGCAAGAACGTGTGAGAGCAGAGGCCCTTCAAGTTTGCCAGGGACACATTCCAGATGCTGAAATGGTTCGCAACATGAAACAGCTAACAATGGTGATTCATGAATCATTGCGCCTATATCCCCCGGTTAGTGTGGTGTCAAGGGAGGCTTTCAAGGACATGAGGTTCGGGGACATTCAGATTCCAAAGGGTATCAATCTTTGGATAATGTTGATAACATTGCACACTGATCCGGAATTATGGGGATCAGATTCCTGTGCGTTCAACCCCAACAGGTTTGCAAATGGGATCACAGGCGCTTGCAAGCTTCCACACTTGTACATGCCATTCGGAGTTGGTCCTCGAGTGTGTGTCGGACAGAACTTTGCCATGGTTGAACTCAAGGTTCTGATAGCTCTCATAGTATCCAACTTCTCCTTCTCGCTTTCTCCCAAGTACCGCCATGCGCCTACTCTTAGATTGGTCGTAGAGCCAGAACATGGTGTTGATCTCTTGGTGAAGAAGTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

493

Amino Acids

54.61

Weight (kDa)

8.79

Isoelectric Point (pI)

33.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 48 - 479 2.5e-76 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 757
AccB1I GGYRCC 3 cut(s) 699, 830, 939
AccB7I CCANNNNNTGG 1 cut(s) 1448
AccI GTMKAC 1 cut(s) 476
AccII CGCG 1 cut(s) 351
AccIII TCCGGA 1 cut(s) 1201
AciI CCGC 3 cut(s) 351, 868, 1408
AclWI GGATC 4 cut(s) 108, 1193, 1222, 1265
AcoI YGGCCR 1 cut(s) 903
AcsI RAATTY 5 cut(s) 184, 212, 493, 528, 684
AdeI CACNNNGTG 1 cut(s) 939
AfaI GTAC 2 cut(s) 1289, 1406
AfiI CCNNNNNNNGG 2 cut(s) 362, 1448
AflIII ACRYGT 1 cut(s) 979
AgsI TTSAA 4 cut(s) 1001, 1123, 1234, 1346
AjiI CACGTC 1 cut(s) 74
AjnI CCWGG 4 cut(s) 162, 648, 688, 1008
AjuI GAANNNNNNNTTGG 4 cut(s) 884, 916, 1370, 1402
AloI GAACNNNNNNTCC 2 cut(s) 1118, 1150
AluBI AGCT 7 cut(s) 7, 552, 590, 671, 1054, 1276, 1364
AluI AGCT 7 cut(s) 7, 552, 590, 671, 1054, 1276, 1364
Alw21I GWGCWC 1 cut(s) 273
Alw26I GTCTC 1 cut(s) 80
Alw44I GTGCAC 1 cut(s) 269
AlwI GGATC 4 cut(s) 108, 1193, 1222, 1265
AlwNI CAGNNNCTG 1 cut(s) 1028
Ama87I CYCGRG 1 cut(s) 1311
Aor13HI TCCGGA 1 cut(s) 1201
AoxI GGCC 2 cut(s) 903, 993
ApaLI GTGCAC 1 cut(s) 269
ApeKI GCWGC 2 cut(s) 4, 788
ApoI RAATTY 5 cut(s) 184, 212, 493, 528, 684
ArsI GACNNNNNNTTYG 2 cut(s) 1118, 1150
Asp700I GAANNNNTTC 2 cut(s) 214, 1035
AspLEI GCGC 5 cut(s) 351, 411, 1083, 1268, 1417
AspS9I GGNCC 5 cut(s) 143, 353, 732, 994, 1307
AsuC2I CCSGG 2 cut(s) 907, 1094
AsuHPI GGTGA 3 cut(s) 236, 1075, 1477
AsuII TTCGAA 1 cut(s) 959
AvaI CYCGRG 1 cut(s) 1311
AvaII GGWCC 4 cut(s) 143, 353, 732, 1307
BaeGI GKGCMC 1 cut(s) 273
BaeI ACNNNNGTAYC 2 cut(s) 317, 350
BanI GGYRCC 3 cut(s) 699, 830, 939
BauI CACGAG 1 cut(s) 394
Bbv12I GWGCWC 1 cut(s) 273
BbvI GCAGC 2 cut(s) 16, 800
BciT130I CCWGG 4 cut(s) 164, 650, 690, 1010
BciVI GTATCC 1 cut(s) 1384
BcnI CCSGG 2 cut(s) 907, 1094
BcoDI GTCTC 1 cut(s) 80
BfaI CTAG 1 cut(s) 50
BfmI CTRYAG 2 cut(s) 219, 764
BfoI RGCGCY 1 cut(s) 1269
BfuAI ACCTGC 1 cut(s) 757
BfuI GTATCC 1 cut(s) 1384
BglI GCCNNNNNGGC 1 cut(s) 106
BisI GCNGC 2 cut(s) 5, 789
BlsI GCNGC 2 cut(s) 6, 790
Bme1390I CCNGG 6 cut(s) 164, 650, 690, 907, 1010, 1094
Bme18I GGWCC 4 cut(s) 143, 353, 732, 1307
BmeT110I CYCGRG 1 cut(s) 1311
BmgBI CACGTC 1 cut(s) 74
BmgT120I GGNCC 5 cut(s) 143, 353, 732, 994, 1307
BmiI GGNNCC 4 cut(s) 391, 701, 832, 941
BmrFI CCNGG 6 cut(s) 164, 650, 690, 907, 1010, 1094
BmsI GCATC 5 cut(s) 10, 271, 412, 565, 1015
BplI GAGNNNNNCTC 2 cut(s) 737, 769
BpmI CTGGAG 1 cut(s) 632
Bpu10I CCTNAGC 1 cut(s) 848
Bpu14I TTCGAA 1 cut(s) 959
BpuEI CTTGAG 2 cut(s) 568, 1334
BpuMI CCSGG 2 cut(s) 907, 1094
BsaJI CCNNGG 5 cut(s) 163, 312, 1009, 1092, 1338
BsaWI WCCGGW 1 cut(s) 1201
Bsc4I CCNNNNNNNGG 2 cut(s) 362, 1448
Bse3DI GCAATG 2 cut(s) 1076, 1187
BseAI TCCGGA 1 cut(s) 1201
BseBI CCWGG 4 cut(s) 164, 650, 690, 1010
BseDI CCNNGG 5 cut(s) 163, 312, 1009, 1092, 1338
BseGI GGATG 1 cut(s) 308
BseLI CCNNNNNNNGG 2 cut(s) 362, 1448
BseMI GCAATG 2 cut(s) 1076, 1187
BseRI GAGGAG 2 cut(s) 382, 635
BseSI GKGCMC 1 cut(s) 273
BseXI GCAGC 2 cut(s) 16, 800
Bsh1236I CGCG 1 cut(s) 351
BshFI GGCC 2 cut(s) 905, 995
BshNI GGYRCC 3 cut(s) 699, 830, 939
BsiHKAI GWGCWC 1 cut(s) 273
BsiHKCI CYCGRG 1 cut(s) 1311
BsiSI CCGG 3 cut(s) 906, 1094, 1202
BslFI GGGAC 4 cut(s) 22, 305, 1026, 1154
BslI CCNNNNNNNGG 2 cut(s) 362, 1448
BsmAI GTCTC 1 cut(s) 80
BsmBI CGTCTC 1 cut(s) 80
BsmFI GGGAC 4 cut(s) 22, 305, 1026, 1154
BsnI GGCC 2 cut(s) 905, 995
BsoBI CYCGRG 1 cut(s) 1311
Bsp119I TTCGAA 1 cut(s) 959
Bsp1286I GDGCHC 1 cut(s) 273
Bsp13I TCCGGA 1 cut(s) 1201
Bsp1407I TGTACA 1 cut(s) 1287
Bsp143I GATC 5 cut(s) 113, 1198, 1214, 1257, 1456
Bsp19I CCATGG 1 cut(s) 1338
BspACI CCGC 3 cut(s) 351, 868, 1408
BspANI GGCC 2 cut(s) 905, 995
BspEI TCCGGA 1 cut(s) 1201
BspFNI CGCG 1 cut(s) 351
BspHI TCATGA 1 cut(s) 1069
BspLI GGNNCC 4 cut(s) 391, 701, 832, 941
BspMAI CTGCAG 1 cut(s) 768
BspMI ACCTGC 1 cut(s) 757
BspPI GGATC 4 cut(s) 108, 1193, 1222, 1265
BspT104I TTCGAA 1 cut(s) 959
BspT107I GGYRCC 3 cut(s) 699, 830, 939
BsrDI GCAATG 2 cut(s) 1076, 1187
BsrGI TGTACA 1 cut(s) 1287
BssECI CCNNGG 5 cut(s) 163, 312, 1009, 1092, 1338
BssMI GATC 5 cut(s) 113, 1198, 1214, 1257, 1456
BssSI CACGAG 1 cut(s) 394
BssT1I CCWWGG 2 cut(s) 312, 1338
Bst2BI CACGAG 1 cut(s) 394
Bst2UI CCWGG 4 cut(s) 164, 650, 690, 1010
Bst4CI ACNGT 3 cut(s) 36, 506, 842
BstAUI TGTACA 1 cut(s) 1287
BstBI TTCGAA 1 cut(s) 959
BstC8I GCNNGC 2 cut(s) 1270, 1274
BstDEI CTNAG 3 cut(s) 736, 848, 1424
BstDSI CCRYGG 1 cut(s) 1338
BstF5I GGATG 1 cut(s) 308
BstFNI CGCG 1 cut(s) 351
BstH2I RGCGCY 1 cut(s) 1269
BstHHI GCGC 5 cut(s) 351, 411, 1083, 1268, 1417
BstKTI GATC 5 cut(s) 116, 1201, 1217, 1260, 1459
BstMAI GTCTC 1 cut(s) 80
BstMBI GATC 5 cut(s) 113, 1198, 1214, 1257, 1456
BstMWI GCNNNNNNNGC 3 cut(s) 106, 127, 929
BstNI CCWGG 4 cut(s) 164, 650, 690, 1010
BstNSI RCATGY 2 cut(s) 306, 1294
BstSCI CCNGG 6 cut(s) 162, 648, 688, 905, 1008, 1092
BstSFI CTRYAG 2 cut(s) 219, 764
BstSLI GKGCMC 1 cut(s) 273
BstUI CGCG 1 cut(s) 351
BstV1I GCAGC 2 cut(s) 16, 800
BsuI GTATCC 1 cut(s) 1384
BsuRI GGCC 2 cut(s) 905, 995
BtgI CCRYGG 1 cut(s) 1338
BtgZI GCGATG 1 cut(s) 588
BtrI CACGTC 1 cut(s) 74
BtsCI GGATG 1 cut(s) 308
BtsI GCAGTG 1 cut(s) 291
BtsIMutI CAGTG 2 cut(s) 291, 1194
BveI ACCTGC 1 cut(s) 757
Cac8I GCNNGC 2 cut(s) 1270, 1274
CaiI CAGNNNCTG 1 cut(s) 1028
CciI TCATGA 1 cut(s) 1069
CfoI GCGC 5 cut(s) 351, 411, 1083, 1268, 1417
Cfr13I GGNCC 5 cut(s) 143, 353, 732, 994, 1307
Csp6I GTAC 2 cut(s) 1288, 1405
CviQI GTAC 2 cut(s) 1288, 1405
DdeI CTNAG 3 cut(s) 736, 848, 1424
DpnI GATC 5 cut(s) 115, 1200, 1216, 1259, 1458
DpnII GATC 5 cut(s) 113, 1198, 1214, 1257, 1456
DraI TTTAAA 1 cut(s) 343
DraIII CACNNNGTG 1 cut(s) 939
EaeI YGGCCR 1 cut(s) 903
Eco130I CCWWGG 2 cut(s) 312, 1338
Eco47I GGWCC 4 cut(s) 143, 353, 732, 1307
Eco88I CYCGRG 1 cut(s) 1311
EcoO109I RGGNCCY 1 cut(s) 994
EcoRI GAATTC 1 cut(s) 684
EcoRII CCWGG 4 cut(s) 162, 648, 688, 1008
EcoT14I CCWWGG 2 cut(s) 312, 1338
ErhI CCWWGG 2 cut(s) 312, 1338
Esp3I CGTCTC 1 cut(s) 80
FalI AAGNNNNNCTT 2 cut(s) 1102, 1134
FaqI GGGAC 4 cut(s) 22, 305, 1026, 1154
FblI GTMKAC 1 cut(s) 476
Fnu4HI GCNGC 2 cut(s) 5, 789
FokI GGATG 1 cut(s) 295
Fsp4HI GCNGC 2 cut(s) 5, 789
FspBI CTAG 1 cut(s) 50
GlaI GCGC 5 cut(s) 350, 410, 1082, 1267, 1416
GluI GCNGC 2 cut(s) 5, 789
GsuI CTGGAG 1 cut(s) 632
HaeII RGCGCY 1 cut(s) 1269
HaeIII GGCC 2 cut(s) 905, 995
HapII CCGG 3 cut(s) 906, 1094, 1202
HhaI GCGC 5 cut(s) 351, 411, 1083, 1268, 1417
Hin6I GCGC 5 cut(s) 349, 409, 1081, 1266, 1415
HinP1I GCGC 5 cut(s) 349, 409, 1081, 1266, 1415
HindIII AAGCTT 2 cut(s) 669, 1274
HinfI GANTC 6 cut(s) 135, 473, 1066, 1073, 1150, 1220
HpaII CCGG 3 cut(s) 906, 1094, 1202
HphI GGTGA 3 cut(s) 236, 1075, 1477
Hpy166II GTNNAC 2 cut(s) 271, 477
Hpy188I TCNGA 9 cut(s) 489, 535, 633, 683, 1149, 1219, 1301, 1325, 1359
Hpy188III TCNNGA 5 cut(s) 425, 585, 1022, 1070, 1202
Hpy8I GTNNAC 2 cut(s) 271, 477
HpyAV CCTTC 4 cut(s) 713, 769, 1007, 1396
HpyCH4III ACNGT 3 cut(s) 36, 506, 842
HpyCH4IV ACGT 3 cut(s) 73, 332, 979
HpyF10VI GCNNNNNNNGC 3 cut(s) 106, 127, 929
HpyF3I CTNAG 3 cut(s) 736, 848, 1424
HpySE526I ACGT 3 cut(s) 73, 332, 979
HspAI GCGC 5 cut(s) 349, 409, 1081, 1266, 1415
Kpn2I TCCGGA 1 cut(s) 1201
Kzo9I GATC 5 cut(s) 113, 1198, 1214, 1257, 1456
LmnI GCTCC 2 cut(s) 12, 395
Lsp1109I GCAGC 2 cut(s) 16, 800
LweI GCATC 5 cut(s) 10, 271, 412, 565, 1015
MaeI CTAG 1 cut(s) 50
MaeII ACGT 3 cut(s) 73, 332, 979
MaeIII GTNAC 2 cut(s) 30, 842
MalI GATC 5 cut(s) 115, 1200, 1216, 1259, 1458
MboI GATC 5 cut(s) 113, 1198, 1214, 1257, 1456
MhlI GDGCHC 1 cut(s) 273
MlyI GAGTC 1 cut(s) 482
MmeI TCCRAC 3 cut(s) 661, 1303, 1401
MroI TCCGGA 1 cut(s) 1201
MroXI GAANNNNTTC 2 cut(s) 214, 1035
MseI TTAA 5 cut(s) 342, 377, 447, 459, 464
MspI CCGG 3 cut(s) 906, 1094, 1202
MspR9I CCNGG 6 cut(s) 164, 650, 690, 907, 1010, 1094
MvaI CCWGG 4 cut(s) 164, 650, 690, 1010
MvnI CGCG 1 cut(s) 351
MwoI GCNNNNNNNGC 3 cut(s) 106, 127, 929
NciI CCSGG 2 cut(s) 907, 1094
NcoI CCATGG 1 cut(s) 1338
NdeII GATC 5 cut(s) 113, 1198, 1214, 1257, 1456
NlaIV GGNNCC 4 cut(s) 391, 701, 832, 941
NspI RCATGY 2 cut(s) 306, 1294
NspV TTCGAA 1 cut(s) 959
PaeR7I CTCGAG 1 cut(s) 1311
PagI TCATGA 1 cut(s) 1069
PdmI GAANNNNTTC 2 cut(s) 214, 1035
PfeI GAWTC 5 cut(s) 135, 1066, 1073, 1150, 1220
PflMI CCANNNNNTGG 1 cut(s) 1448
PfoI TCCNGGA 2 cut(s) 648, 688
PkrI GCNGC 2 cut(s) 6, 790
PleI GAGTC 1 cut(s) 481
PpsI GAGTC 1 cut(s) 481
Psp6I CCWGG 4 cut(s) 162, 648, 688, 1008
PspGI CCWGG 4 cut(s) 162, 648, 688, 1008
PspN4I GGNNCC 4 cut(s) 391, 701, 832, 941
PspPI GGNCC 5 cut(s) 143, 353, 732, 994, 1307
PspXI VCTCGAGB 1 cut(s) 1311
PstI CTGCAG 1 cut(s) 768
PstNI CAGNNNCTG 1 cut(s) 1028
RsaI GTAC 2 cut(s) 1289, 1406
RsaNI GTAC 2 cut(s) 1288, 1405
SaqAI TTAA 5 cut(s) 342, 377, 447, 459, 464
SatI GCNGC 2 cut(s) 5, 789
Sau3AI GATC 5 cut(s) 113, 1198, 1214, 1257, 1456
Sau96I GGNCC 5 cut(s) 143, 353, 732, 994, 1307
SchI GAGTC 1 cut(s) 482
ScrFI CCNGG 6 cut(s) 164, 650, 690, 907, 1010, 1094
SduI GDGCHC 1 cut(s) 273
SfaNI GCATC 5 cut(s) 10, 271, 412, 565, 1015
SfcI CTRYAG 2 cut(s) 219, 764
Sfr274I CTCGAG 1 cut(s) 1311
SfuI TTCGAA 1 cut(s) 959
SinI GGWCC 4 cut(s) 143, 353, 732, 1307
SlaI CTCGAG 1 cut(s) 1311
SmlI CTYRAG 3 cut(s) 583, 1311, 1349
SmoI CTYRAG 3 cut(s) 583, 1311, 1349
SsiI CCGC 3 cut(s) 351, 868, 1408
SspI AATATT 1 cut(s) 172
SspMI CTAG 1 cut(s) 50
StyD4I CCNGG 6 cut(s) 162, 648, 688, 905, 1008, 1092
StyI CCWWGG 2 cut(s) 312, 1338
TaaI ACNGT 3 cut(s) 36, 506, 842
TaiI ACGT 3 cut(s) 76, 335, 982
TaqI TCGA 3 cut(s) 112, 959, 1312
TatI WGTACW 1 cut(s) 1287
TfiI GAWTC 5 cut(s) 135, 1066, 1073, 1150, 1220
Tru1I TTAA 5 cut(s) 342, 377, 447, 459, 464
Tru9I TTAA 5 cut(s) 342, 377, 447, 459, 464
TscAI CASTG 2 cut(s) 291, 1201
TseI GCWGC 2 cut(s) 4, 788
TspDTI ATGAA 4 cut(s) 927, 1058, 1061, 1086
TspGWI ACGGA 1 cut(s) 485
TspRI CASTG 2 cut(s) 291, 1201
Van91I CCANNNNNTGG 1 cut(s) 1448
VneI GTGCAC 1 cut(s) 269
VpaK11BI GGWCC 4 cut(s) 143, 353, 732, 1307
XapI RAATTY 5 cut(s) 184, 212, 493, 528, 684
XceI RCATGY 2 cut(s) 306, 1294
XhoI CTCGAG 1 cut(s) 1311
XmiI GTMKAC 1 cut(s) 476
XmnI GAANNNNTTC 2 cut(s) 214, 1035
XspI CTAG 1 cut(s) 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.