MD03G1046700.v1.1

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0001338
Physical Location & Seq
Forward (+)
3755086 .. 3759953
4868 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1046700.v1.1.491

Sequence Viewer

Length: 1548 bp
ATGGAGCTTCTTCAGTTTGATGCGAAAATGTTGATGTCCCTTGTGTTTCTAGGGTTTGTAGGGTTGTTTGTGCGGTTGTATGATGGGCTTGTTGCGAAGCCGAAGAGGCTGAGGTCCTTGCTAACTAAGCAAGGCATCAATGGACCTCCACCAACTCTTCTTCTGGGAAATATTATGGAGATAAAGAAGGCTCGAGGCTCCAGCAATTCTACCAGTGGACTAATCCCCAGCTCTCACAATTGCGCTGCTCTTGTCTTTCCATTTTTTGAGAAGTGGAGGAAGCAATATGGTGAAGTATTTGCGTTTGCACTTGGCAACACACAAATATTGTGTGTGAACCAACCAGATGCAGTGCGAGAGATAACAACATGCACGTCCTTAGACTTGGGGAAGCCAACATATCAATTTAAAGAGCGAGGTCCTCTGCTAGGTCAAGGTATTTTAACCTCAAATGGCCCCTCATGGGTTCACCAACGCAAAGTGATTGCTCCTGAACTATACATGGACAAAGTCAAGGGAATGATTAACCTAATCACAGAGTCTACAACTACCCTGGTAAATTCTTGGAACAGTAAGATTGAAACAGCCGAAGGAGGAATTGCTGAGATAAAAATTGATAGCTACATGAGAAGCTTCTCTGGTGATGTTATCTCAAGAGCTTGTTTTGGAAGCAACTATTCCAAAGGAGAAGAGATATTTCATAAACTAAGAAATCTCCAGGAGGCTATGTCCAAGAAAGTCTTTTCCACCGGAGTTCCTGGGATGAGACTTCTTCCCACAAAGAGCAACAGGGAAGCTTGGGCATTGGAAAAGGAGGCCAGCACTTTAATACTTCGAGTAGTGAAGGAAAGGCAGGCAGCAGGATATGAGAAAGACCTGTTGCAAATGATTCTTGAGGGTGCTATAAACAGTGACCTCAGCCAAGAGGCTACAGATCGATTCATTGTTGATAACTGCAAAAACATATACTTGGCCGGCTATGAAACCACGGCGGTTTCCGCCACGTGGTGCCTCATGTTGTTGGCTTCAAACCCAAAATGGCAAGAAAGTGTGAGAACAGAGGCCCTTCAAGTTTGCCAAGGCTGTATTCCGGATAATGATATGATTCCTAAGATGAAACAGCTAACTATGGTGATTCATGAATCACTGCGCCTATATCCTCCAGTTACCGTGGTGTCAAGGGAGGCCTTTAAGGACATGAAATTTGGGGACATTAACATCCCAAAGGGTGTCAATGTTTGGACCACAGTGGCAACCCTACACACTGATCCAGAAATATGGGGCCCAGATGCCTATGTGTTCAACCCAGATAGGTTTGCAAATGGGATTACAGGCGCTTGCAAGCTTCCACACGTGTACATGCCATTTGGGATGGGACCCCGAGTGTGTCTTGGACAGAACTTGGCCATGGTTGAACTCAAGATTCTGATAGCTCTCTTAGTTTCCAACTTCTCCTTCTCACTCTCTCCCAAGTACAGACATGGACCTGCTCTTAGATTGGTTATAGAGCCTGAGCATGGAGTCGATCTCCTAGTGAGGAAGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

516

Amino Acids

57.35

Weight (kDa)

8.91

Isoelectric Point (pI)

38.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 87 - 493 1.6e-81 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1495
AccB1I GGYRCC 1 cut(s) 1008
AccI GTMKAC 1 cut(s) 542
AccIII TCCGGA 1 cut(s) 1090
AciI CCGC 3 cut(s) 73, 992, 999
AclWI GGATC 1 cut(s) 1262
AcoI YGGCCR 2 cut(s) 972, 1404
AcsI RAATTY 2 cut(s) 559, 1202
AcvI CACGTG 2 cut(s) 1005, 1354
AdeI CACNNNGTG 1 cut(s) 1008
AfaI GTAC 2 cut(s) 1358, 1475
AfiI CCNNNNNNNGG 4 cut(s) 428, 463, 1005, 1517
AflIII ACRYGT 2 cut(s) 1351, 1353
AgsI TTSAA 5 cut(s) 581, 1029, 1070, 1303, 1415
AjiI CACGTC 1 cut(s) 375
AjnI CCWGG 3 cut(s) 552, 717, 757
AjuI GAANNNNNNNTTGG 4 cut(s) 1071, 1103, 1439, 1471
Alw26I GTCTC 1 cut(s) 760
AlwI GGATC 1 cut(s) 1262
Ama87I CYCGRG 2 cut(s) 192, 1380
Aor13HI TCCGGA 1 cut(s) 1090
AoxI GGCC 7 cut(s) 454, 816, 972, 1062, 1185, 1282, 1404
ApaI GGGCCC 1 cut(s) 1286
ApeKI GCWGC 2 cut(s) 245, 857
ApoI RAATTY 2 cut(s) 559, 1202
ArsI GACNNNNNNTTYG 2 cut(s) 1187, 1219
AspLEI GCGC 3 cut(s) 245, 1152, 1337
AsuHPI GGTGA 4 cut(s) 302, 461, 653, 1144
AvaI CYCGRG 2 cut(s) 192, 1380
AvaII GGWCC 6 cut(s) 114, 143, 419, 1242, 1376, 1484
BaeGI GKGCMC 1 cut(s) 1286
BalI TGGCCA 1 cut(s) 1406
BanI GGYRCC 1 cut(s) 1008
BanII GRGCYC 1 cut(s) 1286
BbrPI CACGTG 2 cut(s) 1005, 1354
BbvCI CCTCAGC 2 cut(s) 110, 917
BbvI GCAGC 2 cut(s) 232, 869
BccI CCATC 2 cut(s) 77, 1366
BceAI ACGGC 1 cut(s) 1005
BciT130I CCWGG 3 cut(s) 554, 719, 759
BcoDI GTCTC 1 cut(s) 760
BfaI CTAG 3 cut(s) 50, 428, 1532
BfmI CTRYAG 1 cut(s) 930
BfoI RGCGCY 1 cut(s) 1338
BfuAI ACCTGC 1 cut(s) 1495
BglI GCCNNNNNGGC 1 cut(s) 106
BisI GCNGC 2 cut(s) 246, 858
BlsI GCNGC 2 cut(s) 247, 859
Bme1390I CCNGG 3 cut(s) 554, 719, 759
Bme18I GGWCC 6 cut(s) 114, 143, 419, 1242, 1376, 1484
BmeT110I CYCGRG 2 cut(s) 192, 1380
BmgBI CACGTC 1 cut(s) 375
BmiI GGNNCC 7 cut(s) 199, 457, 1010, 1283, 1284, 1377, 1378
BmrFI CCNGG 3 cut(s) 554, 719, 759
BmsI GCATC 4 cut(s) 10, 144, 337, 1279
BplI GAGNNNNNCTC 2 cut(s) 1512, 1544
BpmI CTGGAG 3 cut(s) 184, 701, 1146
Bpu10I CCTNAGC 3 cut(s) 110, 917, 1512
BpuEI CTTGAG 3 cut(s) 637, 914, 1403
Bsa29I ATCGAT 1 cut(s) 937
BsaAI YACGTR 2 cut(s) 1005, 1354
BsaJI CCNNGG 6 cut(s) 552, 758, 987, 1078, 1170, 1407
BsaWI WCCGGW 2 cut(s) 749, 1090
BsaXI ACNNNNNCTCC 2 cut(s) 713, 743
Bsc4I CCNNNNNNNGG 4 cut(s) 428, 463, 1005, 1517
Bse118I RCCGGY 1 cut(s) 974
Bse1I ACTGG 2 cut(s) 213, 1163
BseAI TCCGGA 1 cut(s) 1090
BseBI CCWGG 3 cut(s) 554, 719, 759
BseCI ATCGAT 1 cut(s) 937
BseDI CCNNGG 6 cut(s) 552, 758, 987, 1078, 1170, 1407
BseGI GGATG 3 cut(s) 768, 1218, 1377
BseLI CCNNNNNNNGG 4 cut(s) 428, 463, 1005, 1517
BseMII CTCAG 4 cut(s) 101, 594, 931, 1503
BseNI ACTGG 2 cut(s) 213, 1163
BseSI GKGCMC 1 cut(s) 1286
BseXI GCAGC 2 cut(s) 232, 869
BseYI CCCAGC 1 cut(s) 227
BshFI GGCC 7 cut(s) 456, 818, 974, 1064, 1187, 1284, 1406
BshNI GGYRCC 1 cut(s) 1008
BshVI ATCGAT 1 cut(s) 937
BsiHKCI CYCGRG 2 cut(s) 192, 1380
BsiSI CCGG 3 cut(s) 750, 975, 1091
BslFI GGGAC 3 cut(s) 22, 1223, 1389
BslI CCNNNNNNNGG 4 cut(s) 428, 463, 1005, 1517
BsmAI GTCTC 1 cut(s) 760
BsmFI GGGAC 3 cut(s) 22, 1223, 1389
BsnI GGCC 7 cut(s) 456, 818, 974, 1064, 1187, 1284, 1406
BsoBI CYCGRG 2 cut(s) 192, 1380
Bsp120I GGGCCC 1 cut(s) 1282
Bsp1286I GDGCHC 1 cut(s) 1286
Bsp13I TCCGGA 1 cut(s) 1090
Bsp1407I TGTACA 1 cut(s) 1356
Bsp143I GATC 3 cut(s) 934, 1267, 1525
Bsp19I CCATGG 1 cut(s) 1407
BspACI CCGC 3 cut(s) 73, 992, 999
BspANI GGCC 7 cut(s) 456, 818, 974, 1064, 1187, 1284, 1406
BspCNI CTCAG 4 cut(s) 102, 595, 930, 1504
BspDI ATCGAT 1 cut(s) 937
BspEI TCCGGA 1 cut(s) 1090
BspHI TCATGA 1 cut(s) 1138
BspLI GGNNCC 7 cut(s) 199, 457, 1010, 1283, 1284, 1377, 1378
BspMI ACCTGC 1 cut(s) 1495
BspPI GGATC 1 cut(s) 1262
BspT107I GGYRCC 1 cut(s) 1008
BsrFI RCCGGY 1 cut(s) 974
BsrGI TGTACA 1 cut(s) 1356
BsrI ACTGG 2 cut(s) 213, 1163
BssAI RCCGGY 1 cut(s) 974
BssECI CCNNGG 6 cut(s) 552, 758, 987, 1078, 1170, 1407
BssMI GATC 3 cut(s) 934, 1267, 1525
BssT1I CCWWGG 2 cut(s) 1078, 1407
Bst2UI CCWGG 3 cut(s) 554, 719, 759
Bst4CI ACNGT 4 cut(s) 572, 911, 1171, 1249
Bst6I CTCTTC 3 cut(s) 98, 162, 684
BstAUI TGTACA 1 cut(s) 1356
BstBAI YACGTR 2 cut(s) 1005, 1354
BstC8I GCNNGC 5 cut(s) 820, 855, 976, 1339, 1343
BstDSI CCRYGG 3 cut(s) 987, 1170, 1407
BstENI CCTNNNNNAGG 1 cut(s) 426
BstF5I GGATG 3 cut(s) 768, 1218, 1377
BstH2I RGCGCY 1 cut(s) 1338
BstHHI GCGC 3 cut(s) 245, 1152, 1337
BstKTI GATC 3 cut(s) 937, 1270, 1528
BstMAI GTCTC 1 cut(s) 760
BstMBI GATC 3 cut(s) 934, 1267, 1525
BstMWI GCNNNNNNNGC 3 cut(s) 106, 127, 998
BstNI CCWGG 3 cut(s) 554, 719, 759
BstNSI RCATGY 2 cut(s) 372, 1363
BstSCI CCNGG 3 cut(s) 552, 717, 757
BstSFI CTRYAG 1 cut(s) 930
BstSLI GKGCMC 1 cut(s) 1286
BstV1I GCAGC 2 cut(s) 232, 869
BstXI CCANNNNNNTGG 1 cut(s) 1278
Bsu15I ATCGAT 1 cut(s) 937
BsuRI GGCC 7 cut(s) 456, 818, 974, 1064, 1187, 1284, 1406
BsuTUI ATCGAT 1 cut(s) 937
BtgI CCRYGG 3 cut(s) 987, 1170, 1407
BtrI CACGTC 1 cut(s) 375
BtsCI GGATG 3 cut(s) 768, 1218, 1377
BtsI GCAGTG 2 cut(s) 357, 1145
BtsIMutI CAGTG 6 cut(s) 220, 357, 916, 1145, 1254, 1263
BveI ACCTGC 1 cut(s) 1495
Cac8I GCNNGC 5 cut(s) 820, 855, 976, 1339, 1343
CciI TCATGA 1 cut(s) 1138
CfoI GCGC 3 cut(s) 245, 1152, 1337
Cfr10I RCCGGY 1 cut(s) 974
ClaI ATCGAT 1 cut(s) 937
Csp6I GTAC 2 cut(s) 1357, 1474
CviQI GTAC 2 cut(s) 1357, 1474
DpnI GATC 3 cut(s) 936, 1269, 1527
DpnII GATC 3 cut(s) 934, 1267, 1525
DraI TTTAAA 1 cut(s) 409
DraIII CACNNNGTG 1 cut(s) 1008
EaeI YGGCCR 2 cut(s) 972, 1404
Eam1104I CTCTTC 3 cut(s) 98, 162, 684
EarI CTCTTC 3 cut(s) 98, 162, 684
EciI GGCGGA 1 cut(s) 988
Eco130I CCWWGG 2 cut(s) 1078, 1407
Eco147I AGGCCT 1 cut(s) 1187
Eco24I GRGCYC 1 cut(s) 1286
Eco47I GGWCC 6 cut(s) 114, 143, 419, 1242, 1376, 1484
Eco72I CACGTG 2 cut(s) 1005, 1354
Eco88I CYCGRG 2 cut(s) 192, 1380
EcoNI CCTNNNNNAGG 1 cut(s) 426
EcoO109I RGGNCCY 5 cut(s) 114, 419, 1063, 1282, 1376
EcoRII CCWGG 3 cut(s) 552, 717, 757
EcoT14I CCWWGG 2 cut(s) 1078, 1407
EcoT38I GRGCYC 1 cut(s) 1286
ErhI CCWWGG 2 cut(s) 1078, 1407
FalI AAGNNNNNCTT 2 cut(s) 725, 757
FaqI GGGAC 3 cut(s) 22, 1223, 1389
FblI GTMKAC 1 cut(s) 542
Fnu4HI GCNGC 2 cut(s) 246, 858
FokI GGATG 3 cut(s) 775, 1205, 1384
FriOI GRGCYC 1 cut(s) 1286
Fsp4HI GCNGC 2 cut(s) 246, 858
FspBI CTAG 3 cut(s) 50, 428, 1532
GlaI GCGC 3 cut(s) 244, 1151, 1336
GluI GCNGC 2 cut(s) 246, 858
GsaI CCCAGC 1 cut(s) 231
GsuI CTGGAG 3 cut(s) 184, 701, 1146
HaeII RGCGCY 1 cut(s) 1338
HaeIII GGCC 7 cut(s) 456, 818, 974, 1064, 1187, 1284, 1406
HapII CCGG 3 cut(s) 750, 975, 1091
HhaI GCGC 3 cut(s) 245, 1152, 1337
Hin6I GCGC 3 cut(s) 243, 1150, 1335
HinP1I GCGC 3 cut(s) 243, 1150, 1335
HindIII AAGCTT 3 cut(s) 631, 795, 1343
HinfI GANTC 8 cut(s) 539, 889, 939, 1105, 1135, 1142, 1423, 1521
HpaII CCGG 3 cut(s) 750, 975, 1091
HphI GGTGA 4 cut(s) 302, 461, 653, 1144
Hpy166II GTNNAC 5 cut(s) 218, 337, 469, 543, 1357
Hpy188I TCNGA 1 cut(s) 1428
Hpy188III TCNNGA 7 cut(s) 491, 654, 893, 1091, 1139, 1271, 1420
Hpy8I GTNNAC 5 cut(s) 218, 337, 469, 543, 1357
HpyAV CCTTC 5 cut(s) 181, 584, 838, 1076, 1465
HpyCH4III ACNGT 4 cut(s) 572, 911, 1171, 1249
HpyCH4IV ACGT 3 cut(s) 374, 1004, 1353
HpyCH4V TGCA 7 cut(s) 308, 350, 372, 883, 957, 1319, 1341
HpyF10VI GCNNNNNNNGC 3 cut(s) 106, 127, 998
HpySE526I ACGT 3 cut(s) 374, 1004, 1353
HspAI GCGC 3 cut(s) 243, 1150, 1335
KflI GGGWCCC 1 cut(s) 1376
Kpn2I TCCGGA 1 cut(s) 1090
KroI GCCGGC 1 cut(s) 974
KroNI GCCGGC 1 cut(s) 976
Kzo9I GATC 3 cut(s) 934, 1267, 1525
LmnI GCTCC 3 cut(s) 4, 203, 493
Lsp1109I GCAGC 2 cut(s) 232, 869
LweI GCATC 4 cut(s) 10, 144, 337, 1279
MaeI CTAG 3 cut(s) 50, 428, 1532
MaeII ACGT 3 cut(s) 374, 1004, 1353
MaeIII GTNAC 2 cut(s) 911, 1165
MalI GATC 3 cut(s) 936, 1269, 1527
MboI GATC 3 cut(s) 934, 1267, 1525
MboII GAAGA 5 cut(s) 115, 149, 152, 701, 764
MfeI CAATTG 1 cut(s) 238
MhlI GDGCHC 1 cut(s) 1286
MlsI TGGCCA 1 cut(s) 1406
MluCI AATT 7 cut(s) 205, 238, 404, 559, 597, 612, 1202
MluNI TGGCCA 1 cut(s) 1406
MlyI GAGTC 2 cut(s) 548, 1530
MmeI TCCRAC 1 cut(s) 1470
Mox20I TGGCCA 1 cut(s) 1406
MroI TCCGGA 1 cut(s) 1090
MroNI GCCGGC 1 cut(s) 974
MscI TGGCCA 1 cut(s) 1406
MseI TTAA 6 cut(s) 408, 443, 525, 827, 1191, 1215
Msp20I TGGCCA 1 cut(s) 1406
MspI CCGG 3 cut(s) 750, 975, 1091
MspR9I CCNGG 3 cut(s) 554, 719, 759
MunI CAATTG 1 cut(s) 238
MvaI CCWGG 3 cut(s) 554, 719, 759
MwoI GCNNNNNNNGC 3 cut(s) 106, 127, 998
NaeI GCCGGC 1 cut(s) 976
NcoI CCATGG 1 cut(s) 1407
NdeII GATC 3 cut(s) 934, 1267, 1525
NgoMIV GCCGGC 1 cut(s) 974
NlaIV GGNNCC 7 cut(s) 199, 457, 1010, 1283, 1284, 1377, 1378
NmuCI GTSAC 1 cut(s) 911
NspI RCATGY 2 cut(s) 372, 1363
PaeR7I CTCGAG 1 cut(s) 192
PagI TCATGA 1 cut(s) 1138
PceI AGGCCT 1 cut(s) 1187
PdiI GCCGGC 1 cut(s) 976
PfeI GAWTC 6 cut(s) 889, 939, 1105, 1135, 1142, 1423
PflFI GACNNNGTC 1 cut(s) 509
PfoI TCCNGGA 1 cut(s) 717
PkrI GCNGC 2 cut(s) 247, 859
PleI GAGTC 2 cut(s) 547, 1529
PmaCI CACGTG 2 cut(s) 1005, 1354
PmlI CACGTG 2 cut(s) 1005, 1354
PpsI GAGTC 2 cut(s) 547, 1529
Ppu21I YACGTR 2 cut(s) 1005, 1354
PpuMI RGGWCCY 3 cut(s) 114, 419, 1376
Psp5II RGGWCCY 3 cut(s) 114, 419, 1376
Psp6I CCWGG 3 cut(s) 552, 717, 757
PspCI CACGTG 2 cut(s) 1005, 1354
PspFI CCCAGC 1 cut(s) 227
PspGI CCWGG 3 cut(s) 552, 717, 757
PspN4I GGNNCC 7 cut(s) 199, 457, 1010, 1283, 1284, 1377, 1378
PspOMI GGGCCC 1 cut(s) 1282
PspPPI RGGWCCY 3 cut(s) 114, 419, 1376
PspXI VCTCGAGB 1 cut(s) 192
PsyI GACNNNGTC 1 cut(s) 509
RsaI GTAC 2 cut(s) 1358, 1475
RsaNI GTAC 2 cut(s) 1357, 1474
SaqAI TTAA 6 cut(s) 408, 443, 525, 827, 1191, 1215
SatI GCNGC 2 cut(s) 246, 858
Sau3AI GATC 3 cut(s) 934, 1267, 1525
SchI GAGTC 2 cut(s) 548, 1530
ScrFI CCNGG 3 cut(s) 554, 719, 759
SduI GDGCHC 1 cut(s) 1286
SfaNI GCATC 4 cut(s) 10, 144, 337, 1279
SfcI CTRYAG 1 cut(s) 930
Sfr274I CTCGAG 1 cut(s) 192
SinI GGWCC 6 cut(s) 114, 143, 419, 1242, 1376, 1484
SlaI CTCGAG 1 cut(s) 192
SmlI CTYRAG 4 cut(s) 192, 652, 893, 1418
SmoI CTYRAG 4 cut(s) 192, 652, 893, 1418
Sse9I AATT 7 cut(s) 205, 238, 404, 559, 597, 612, 1202
SseBI AGGCCT 1 cut(s) 1187
SsiI CCGC 3 cut(s) 73, 992, 999
SspI AATATT 2 cut(s) 172, 327
SspMI CTAG 3 cut(s) 50, 428, 1532
StuI AGGCCT 1 cut(s) 1187
StyD4I CCNGG 3 cut(s) 552, 717, 757
StyI CCWWGG 2 cut(s) 1078, 1407
TaaI ACNGT 4 cut(s) 572, 911, 1171, 1249
TaiI ACGT 3 cut(s) 377, 1007, 1356
TaqI TCGA 4 cut(s) 193, 835, 937, 1524
TasI AATT 7 cut(s) 205, 238, 404, 559, 597, 612, 1202
TatI WGTACW 2 cut(s) 1356, 1473
TfiI GAWTC 6 cut(s) 889, 939, 1105, 1135, 1142, 1423
Tru1I TTAA 6 cut(s) 408, 443, 525, 827, 1191, 1215
Tru9I TTAA 6 cut(s) 408, 443, 525, 827, 1191, 1215
TscAI CASTG 6 cut(s) 220, 357, 916, 1152, 1254, 1270
TseFI GTSAC 1 cut(s) 911
TseI GCWGC 2 cut(s) 245, 857
Tsp45I GTSAC 1 cut(s) 911
TspDTI ATGAA 7 cut(s) 689, 931, 996, 1127, 1130, 1155, 1214
TspRI CASTG 6 cut(s) 220, 357, 916, 1152, 1254, 1270
Tth111I GACNNNGTC 1 cut(s) 509
VpaK11BI GGWCC 6 cut(s) 114, 143, 419, 1242, 1376, 1484
XagI CCTNNNNNAGG 1 cut(s) 426
XapI RAATTY 2 cut(s) 559, 1202
XceI RCATGY 2 cut(s) 372, 1363
XhoI CTCGAG 1 cut(s) 192
XmiI GTMKAC 1 cut(s) 542
XspI CTAG 3 cut(s) 50, 428, 1532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.