MD03G1046900.v1.1

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0001338
Physical Location & Seq
Forward (+)
3779645 .. 3782898
3254 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1046900.v1.1.491

Sequence Viewer

Length: 1542 bp
ATGCAGCTCCTCCAATTTGATGCAAAAGTGTTACTGTCCCTTGTGTTCCTAGGGTTTATAGGATTGGTGGCACGTCTCTACAACGGGCTTGTTGCGAAGCCAAAGAGGCTTCGATCCTTGTTAACCAAGCAAGGAATTAATGGACCTCCACCTACTATTCTCCTGGGAAATATTTTGGAAATAAAGAAGGCACGAGGCTCCATCAATCCTACAAGTGAACCCCCGACCTCTCACAACTGTGCTGCTCTTCTCTTTCCATTTTTAGAGAAGTGGAGGAAGCTATATGGTGAAGTATTTGTGTTTGCACTTGGAAACACACAAATATTATATGTGAACCAGCCTGACGCAGTGAGGGACATTACAACATGCACATCCTTGGACTTGGGGAAACCAACGTATCAATTTAAAGAGCGCGGTCCTTTGCTTGGTCAAGGTATTTTAACCTCAAATGGCACCTCGTGGGTGCATCAGCGCAAAATCATTGCTCCTGAACTATACATGGAGAAAGTTAAGGGAATGGTTAATTTAATCACGGAGTCTACAATTACTCTGATAAATTCGTGGAACAGTAGAATCGAGGCAGAGGGAGGACTTTCTGACATGAAAATAGACAGCTACATTGGAAGCTTCTCTGGCGATGCTATCTCAAGAGCTTGTTTTGGAAGCAACTATTCCAAAGGGGAGGAGATATTTCAGAAACTAAAGCAAATCCAGGAGGCAATGGGGAGGAAAGCTTTTCTGGCCGGAATTCCTGGAATGAGGCACCTTCCCACTAAGAGCAATAGGGAAGTTTGGGCCTTAGAAAAGGAGGCTAGCGCTTTGATACTGCAGGTAGTGAAGGAAAGGCGGGCAGCAGAAAATGAGAAAGACTTATTGCAAATTATTCTTGAGAGTGCTAAAAACAGTAACCTTAGCCAAGAGGCAACAGACCGCTTCATCGTTGATAACTGCAAGAACATATACTTGGCCGGGTATGAAACCACAGCAGTTGCAGCCACATGGTGCCTCATGCTGTTGGCTTCGAACCAAGAATGGCAAGAACGTGTGAGAGCGGAGGCCCTTCAAGTTTGCCAGGGACACATTCCAGATGCTGATATGGTTCGCAACATGAAACAGCTAACAATGGTGATTCATGAATCATTGCGCCTATATCCCCCGGTTAGTGTGGTGTCAAGGGAGGCTTTCAAGGACATGAAGTTCGGGGACATTCATGTTCCAAAGGGTATCAATGTTTGGACAATGGTGATAACATTGCACACTGATCCGAAACTATGGGGACCAGATTCTTATGCGTTCAACCCCAATAGGTTTGCAAATGGAATCACAGGTGCTTGCAAGCTTCCACAGTTGTACATGCCATTCGGAGTTGGTCCTCGAGTGTGTCTTGGACAGTACTTTGCCATGGTTGAACTCAAGGTTCTGATAGCTCTCATAGTATCCAACTTCTCCTTCTCCCTTTCTCCCAAGTACCGCCATGCTCCTACTCTTAGATTGGTCGTAGAGCCGGAACATGGTGTTGATCTCTTGGTGAAGAAGTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

514

Amino Acids

57.31

Weight (kDa)

9.17

Isoelectric Point (pI)

33.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 87 - 500 1.6e-81 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1540
Acc36I ACCTGC 1 cut(s) 820
AccB1I GGYRCC 3 cut(s) 452, 762, 1002
AccBSI CCGCTC 1 cut(s) 1052
AccI GTMKAC 1 cut(s) 539
AccII CGCG 1 cut(s) 414
AciI CCGC 5 cut(s) 414, 847, 931, 1052, 1471
AclWI GGATC 2 cut(s) 108, 1256
AcoI YGGCCR 2 cut(s) 741, 966
AcsI RAATTY 2 cut(s) 556, 747
AdeI CACNNNGTG 2 cut(s) 459, 1002
AfaI GTAC 3 cut(s) 1352, 1394, 1469
AfeI AGCGCT 1 cut(s) 817
AfiI CCNNNNNNNGG 2 cut(s) 425, 1511
AflIII ACRYGT 1 cut(s) 1042
AgsI TTSAA 4 cut(s) 1064, 1186, 1297, 1409
AjiI CACGTC 1 cut(s) 74
AjnI CCWGG 4 cut(s) 162, 711, 751, 1071
AjuI GAANNNNNNNTTGG 4 cut(s) 947, 979, 1433, 1465
AleI CACNNNNGTG 1 cut(s) 237
AloI GAACNNNNNNTCC 2 cut(s) 1181, 1213
AluBI AGCT 9 cut(s) 7, 280, 615, 627, 653, 734, 1117, 1339, 1427
AluI AGCT 9 cut(s) 7, 280, 615, 627, 653, 734, 1117, 1339, 1427
Alw26I GTCTC 1 cut(s) 80
AlwI GGATC 2 cut(s) 108, 1256
AlwNI CAGNNNCTG 1 cut(s) 1091
Ama87I CYCGRG 1 cut(s) 1374
Aor51HI AGCGCT 1 cut(s) 817
AoxI GGCC 4 cut(s) 741, 795, 966, 1056
ApeKI GCWGC 4 cut(s) 4, 242, 851, 992
ApoI RAATTY 2 cut(s) 556, 747
ArsI GACNNNNNNTTYG 2 cut(s) 1181, 1213
AseI ATTAAT 1 cut(s) 138
AspA2I CCTAGG 1 cut(s) 49
AspLEI GCGC 4 cut(s) 414, 474, 818, 1146
AspS9I GGNCC 6 cut(s) 143, 416, 795, 1057, 1277, 1370
AsuC2I CCSGG 2 cut(s) 970, 1157
AsuHPI GGTGA 4 cut(s) 299, 1138, 1255, 1540
AsuII TTCGAA 1 cut(s) 1022
AsuNHI GCTAGC 1 cut(s) 812
AvaI CYCGRG 1 cut(s) 1374
AvaII GGWCC 4 cut(s) 143, 416, 1277, 1370
AvrII CCTAGG 1 cut(s) 49
BaeI ACNNNNGTAYC 2 cut(s) 380, 413
BanI GGYRCC 3 cut(s) 452, 762, 1002
BauI CACGAG 2 cut(s) 192, 457
BbvI GCAGC 4 cut(s) 16, 229, 863, 1004
BccI CCATC 1 cut(s) 209
BciT130I CCWGG 4 cut(s) 164, 713, 753, 1073
BciVI GTATCC 1 cut(s) 1447
BcnI CCSGG 2 cut(s) 970, 1157
BcoDI GTCTC 1 cut(s) 80
BfaI CTAG 2 cut(s) 50, 813
BfmI CTRYAG 1 cut(s) 827
BfoI RGCGCY 1 cut(s) 819
BfuAI ACCTGC 1 cut(s) 820
BfuI GTATCC 1 cut(s) 1447
BglI GCCNNNNNGGC 1 cut(s) 106
BisI GCNGC 4 cut(s) 5, 243, 852, 993
BlnI CCTAGG 1 cut(s) 49
BlsI GCNGC 4 cut(s) 6, 244, 853, 994
BmcAI AGTACT 1 cut(s) 1394
Bme1390I CCNGG 6 cut(s) 164, 713, 753, 970, 1073, 1157
Bme18I GGWCC 4 cut(s) 143, 416, 1277, 1370
BmeT110I CYCGRG 1 cut(s) 1374
BmgBI CACGTC 1 cut(s) 74
BmgT120I GGNCC 6 cut(s) 143, 416, 795, 1057, 1277, 1370
BmiI GGNNCC 5 cut(s) 199, 454, 764, 1004, 1278
BmrFI CCNGG 6 cut(s) 164, 713, 753, 970, 1073, 1157
BmsI GCATC 4 cut(s) 10, 475, 628, 1078
BmtI GCTAGC 1 cut(s) 816
Bpu10I CCTNAGC 1 cut(s) 911
Bpu14I TTCGAA 1 cut(s) 1022
BpuEI CTTGAG 3 cut(s) 631, 908, 1397
BpuMI CCSGG 2 cut(s) 970, 1157
BsaJI CCNNGG 6 cut(s) 49, 163, 375, 1072, 1155, 1401
Bsc4I CCNNNNNNNGG 2 cut(s) 425, 1511
Bse3DI GCAATG 4 cut(s) 480, 726, 1139, 1250
BseBI CCWGG 4 cut(s) 164, 713, 753, 1073
BseDI CCNNGG 6 cut(s) 49, 163, 375, 1072, 1155, 1401
BseGI GGATG 1 cut(s) 371
BseLI CCNNNNNNNGG 2 cut(s) 425, 1511
BseMI GCAATG 4 cut(s) 480, 726, 1139, 1250
BseRI GAGGAG 1 cut(s) 698
BseXI GCAGC 4 cut(s) 16, 229, 863, 1004
Bsh1236I CGCG 1 cut(s) 414
BshFI GGCC 4 cut(s) 743, 797, 968, 1058
BshNI GGYRCC 3 cut(s) 452, 762, 1002
BsiHKCI CYCGRG 1 cut(s) 1374
BsiSI CCGG 4 cut(s) 744, 969, 1157, 1505
BslFI GGGAC 5 cut(s) 22, 368, 1089, 1217, 1290
BslI CCNNNNNNNGG 2 cut(s) 425, 1511
BsmAI GTCTC 1 cut(s) 80
BsmBI CGTCTC 1 cut(s) 80
BsmFI GGGAC 5 cut(s) 22, 368, 1089, 1217, 1290
BsnI GGCC 4 cut(s) 743, 797, 968, 1058
BsoBI CYCGRG 1 cut(s) 1374
Bsp119I TTCGAA 1 cut(s) 1022
Bsp1407I TGTACA 1 cut(s) 1350
Bsp143I GATC 3 cut(s) 113, 1261, 1519
Bsp19I CCATGG 1 cut(s) 1401
BspACI CCGC 5 cut(s) 414, 847, 931, 1052, 1471
BspANI GGCC 4 cut(s) 743, 797, 968, 1058
BspFNI CGCG 1 cut(s) 414
BspHI TCATGA 1 cut(s) 1132
BspLI GGNNCC 5 cut(s) 199, 454, 764, 1004, 1278
BspMAI CTGCAG 1 cut(s) 831
BspMI ACCTGC 1 cut(s) 820
BspOI GCTAGC 1 cut(s) 816
BspPI GGATC 2 cut(s) 108, 1256
BspQI GCTCTTC 1 cut(s) 252
BspT104I TTCGAA 1 cut(s) 1022
BspT107I GGYRCC 3 cut(s) 452, 762, 1002
BsrBI CCGCTC 1 cut(s) 1052
BsrDI GCAATG 4 cut(s) 480, 726, 1139, 1250
BsrGI TGTACA 1 cut(s) 1350
BssECI CCNNGG 6 cut(s) 49, 163, 375, 1072, 1155, 1401
BssMI GATC 3 cut(s) 113, 1261, 1519
BssSI CACGAG 2 cut(s) 192, 457
BssT1I CCWWGG 3 cut(s) 49, 375, 1401
Bst2BI CACGAG 2 cut(s) 192, 457
Bst2UI CCWGG 4 cut(s) 164, 713, 753, 1073
Bst4CI ACNGT 6 cut(s) 36, 239, 569, 905, 1347, 1392
Bst6I CTCTTC 1 cut(s) 252
BstAUI TGTACA 1 cut(s) 1350
BstBI TTCGAA 1 cut(s) 1022
BstC8I GCNNGC 4 cut(s) 814, 849, 1333, 1337
BstDEI CTNAG 4 cut(s) 774, 799, 911, 1487
BstDSI CCRYGG 1 cut(s) 1401
BstF5I GGATG 1 cut(s) 371
BstFNI CGCG 1 cut(s) 414
BstH2I RGCGCY 1 cut(s) 819
BstHHI GCGC 4 cut(s) 414, 474, 818, 1146
BstKTI GATC 3 cut(s) 116, 1264, 1522
BstMAI GTCTC 1 cut(s) 80
BstMBI GATC 3 cut(s) 113, 1261, 1519
BstMWI GCNNNNNNNGC 4 cut(s) 106, 633, 740, 992
BstNI CCWGG 4 cut(s) 164, 713, 753, 1073
BstNSI RCATGY 2 cut(s) 369, 1357
BstSCI CCNGG 6 cut(s) 162, 711, 751, 968, 1071, 1155
BstSFI CTRYAG 1 cut(s) 827
BstUI CGCG 1 cut(s) 414
BstV1I GCAGC 4 cut(s) 16, 229, 863, 1004
BsuI GTATCC 1 cut(s) 1447
BsuRI GGCC 4 cut(s) 743, 797, 968, 1058
BtgI CCRYGG 1 cut(s) 1401
BtgZI GCGATG 1 cut(s) 651
BtrI CACGTC 1 cut(s) 74
BtsCI GGATG 1 cut(s) 371
BtsI GCAGTG 1 cut(s) 354
BtsIMutI CAGTG 2 cut(s) 354, 1257
BveI ACCTGC 1 cut(s) 820
Cac8I GCNNGC 4 cut(s) 814, 849, 1333, 1337
CaiI CAGNNNCTG 1 cut(s) 1091
CciI TCATGA 1 cut(s) 1132
CfoI GCGC 4 cut(s) 414, 474, 818, 1146
Cfr13I GGNCC 6 cut(s) 143, 416, 795, 1057, 1277, 1370
CseI GACGC 1 cut(s) 353
Csp6I GTAC 3 cut(s) 1351, 1393, 1468
CspCI CAANNNNNGTGG 2 cut(s) 970, 1005
CviQI GTAC 3 cut(s) 1351, 1393, 1468
DdeI CTNAG 4 cut(s) 774, 799, 911, 1487
DpnI GATC 3 cut(s) 115, 1263, 1521
DpnII GATC 3 cut(s) 113, 1261, 1519
DraI TTTAAA 1 cut(s) 406
DraIII CACNNNGTG 2 cut(s) 459, 1002
EaeI YGGCCR 2 cut(s) 741, 966
Eam1104I CTCTTC 1 cut(s) 252
EarI CTCTTC 1 cut(s) 252
Eco130I CCWWGG 3 cut(s) 49, 375, 1401
Eco47I GGWCC 4 cut(s) 143, 416, 1277, 1370
Eco47III AGCGCT 1 cut(s) 817
Eco88I CYCGRG 1 cut(s) 1374
EcoO109I RGGNCCY 1 cut(s) 1057
EcoRI GAATTC 1 cut(s) 747
EcoRII CCWGG 4 cut(s) 162, 711, 751, 1071
EcoT14I CCWWGG 3 cut(s) 49, 375, 1401
ErhI CCWWGG 3 cut(s) 49, 375, 1401
Esp3I CGTCTC 1 cut(s) 80
FalI AAGNNNNNCTT 2 cut(s) 1165, 1197
FaqI GGGAC 5 cut(s) 22, 368, 1089, 1217, 1290
FauI CCCGC 1 cut(s) 840
FblI GTMKAC 1 cut(s) 539
Fnu4HI GCNGC 4 cut(s) 5, 243, 852, 993
FokI GGATG 1 cut(s) 358
Fsp4HI GCNGC 4 cut(s) 5, 243, 852, 993
FspBI CTAG 2 cut(s) 50, 813
GlaI GCGC 4 cut(s) 413, 473, 817, 1145
GluI GCNGC 4 cut(s) 5, 243, 852, 993
HaeII RGCGCY 1 cut(s) 819
HaeIII GGCC 4 cut(s) 743, 797, 968, 1058
HapII CCGG 4 cut(s) 744, 969, 1157, 1505
HgaI GACGC 1 cut(s) 353
HhaI GCGC 4 cut(s) 414, 474, 818, 1146
Hin6I GCGC 4 cut(s) 412, 472, 816, 1144
HinP1I GCGC 4 cut(s) 412, 472, 816, 1144
HincII GTYRAC 1 cut(s) 123
HindII GTYRAC 1 cut(s) 123
HindIII AAGCTT 3 cut(s) 625, 732, 1337
HinfI GANTC 6 cut(s) 536, 573, 1129, 1136, 1283, 1320
HpaI GTTAAC 1 cut(s) 123
HpaII CCGG 4 cut(s) 744, 969, 1157, 1505
HphI GGTGA 4 cut(s) 299, 1138, 1255, 1540
Hpy166II GTNNAC 4 cut(s) 123, 218, 334, 540
Hpy188I TCNGA 6 cut(s) 552, 598, 696, 1266, 1364, 1422
Hpy188III TCNNGA 5 cut(s) 488, 648, 887, 1085, 1133
Hpy8I GTNNAC 4 cut(s) 123, 218, 334, 540
HpyAV CCTTC 5 cut(s) 181, 776, 832, 1070, 1459
HpyCH4III ACNGT 6 cut(s) 36, 239, 569, 905, 1347, 1392
HpyCH4IV ACGT 3 cut(s) 73, 395, 1042
HpyF10VI GCNNNNNNNGC 4 cut(s) 106, 633, 740, 992
HpyF3I CTNAG 4 cut(s) 774, 799, 911, 1487
HpySE526I ACGT 3 cut(s) 73, 395, 1042
HspAI GCGC 4 cut(s) 412, 472, 816, 1144
KspAI GTTAAC 1 cut(s) 123
Kzo9I GATC 3 cut(s) 113, 1261, 1519
LguI GCTCTTC 1 cut(s) 252
LmnI GCTCC 4 cut(s) 12, 203, 490, 1483
Lsp1109I GCAGC 4 cut(s) 16, 229, 863, 1004
LweI GCATC 4 cut(s) 10, 475, 628, 1078
MaeI CTAG 2 cut(s) 50, 813
MaeII ACGT 3 cut(s) 73, 395, 1042
MaeIII GTNAC 2 cut(s) 30, 905
MalI GATC 3 cut(s) 115, 1263, 1521
MbiI CCGCTC 1 cut(s) 1052
MboI GATC 3 cut(s) 113, 1261, 1519
MboII GAAGA 1 cut(s) 239
MluCI AATT 8 cut(s) 14, 135, 401, 523, 543, 556, 747, 879
MlyI GAGTC 1 cut(s) 545
MmeI TCCRAC 1 cut(s) 1464
MseI TTAA 7 cut(s) 122, 138, 405, 440, 510, 522, 527
MslI CAYNNNNRTG 1 cut(s) 237
MspI CCGG 4 cut(s) 744, 969, 1157, 1505
MspR9I CCNGG 6 cut(s) 164, 713, 753, 970, 1073, 1157
MvaI CCWGG 4 cut(s) 164, 713, 753, 1073
MvnI CGCG 1 cut(s) 414
MwoI GCNNNNNNNGC 4 cut(s) 106, 633, 740, 992
NciI CCSGG 2 cut(s) 970, 1157
NcoI CCATGG 1 cut(s) 1401
NdeII GATC 3 cut(s) 113, 1261, 1519
NheI GCTAGC 1 cut(s) 812
NlaIV GGNNCC 5 cut(s) 199, 454, 764, 1004, 1278
NspI RCATGY 2 cut(s) 369, 1357
NspV TTCGAA 1 cut(s) 1022
OliI CACNNNNGTG 1 cut(s) 237
PaeR7I CTCGAG 1 cut(s) 1374
PagI TCATGA 1 cut(s) 1132
PciSI GCTCTTC 1 cut(s) 252
PfeI GAWTC 5 cut(s) 573, 1129, 1136, 1283, 1320
PfoI TCCNGGA 2 cut(s) 711, 751
PkrI GCNGC 4 cut(s) 6, 244, 853, 994
PleI GAGTC 1 cut(s) 544
PpsI GAGTC 1 cut(s) 544
PshBI ATTAAT 1 cut(s) 138
PsiI TTATAA 1 cut(s) 1540
Psp6I CCWGG 4 cut(s) 162, 711, 751, 1071
PspGI CCWGG 4 cut(s) 162, 711, 751, 1071
PspN4I GGNNCC 5 cut(s) 199, 454, 764, 1004, 1278
PspPI GGNCC 6 cut(s) 143, 416, 795, 1057, 1277, 1370
PspXI VCTCGAGB 1 cut(s) 1374
PstI CTGCAG 1 cut(s) 831
PstNI CAGNNNCTG 1 cut(s) 1091
RsaI GTAC 3 cut(s) 1352, 1394, 1469
RsaNI GTAC 3 cut(s) 1351, 1393, 1468
RseI CAYNNNNRTG 1 cut(s) 237
SapI GCTCTTC 1 cut(s) 252
SaqAI TTAA 7 cut(s) 122, 138, 405, 440, 510, 522, 527
SatI GCNGC 4 cut(s) 5, 243, 852, 993
Sau3AI GATC 3 cut(s) 113, 1261, 1519
Sau96I GGNCC 6 cut(s) 143, 416, 795, 1057, 1277, 1370
ScaI AGTACT 1 cut(s) 1394
SchI GAGTC 1 cut(s) 545
ScrFI CCNGG 6 cut(s) 164, 713, 753, 970, 1073, 1157
SfaNI GCATC 4 cut(s) 10, 475, 628, 1078
SfcI CTRYAG 1 cut(s) 827
Sfr274I CTCGAG 1 cut(s) 1374
SfuI TTCGAA 1 cut(s) 1022
SinI GGWCC 4 cut(s) 143, 416, 1277, 1370
SlaI CTCGAG 1 cut(s) 1374
SmiMI CAYNNNNRTG 1 cut(s) 237
SmlI CTYRAG 4 cut(s) 646, 887, 1374, 1412
SmoI CTYRAG 4 cut(s) 646, 887, 1374, 1412
Sse9I AATT 8 cut(s) 14, 135, 401, 523, 543, 556, 747, 879
SsiI CCGC 5 cut(s) 414, 847, 931, 1052, 1471
SspI AATATT 2 cut(s) 172, 324
SspMI CTAG 2 cut(s) 50, 813
StyD4I CCNGG 6 cut(s) 162, 711, 751, 968, 1071, 1155
StyI CCWWGG 3 cut(s) 49, 375, 1401
TaaI ACNGT 6 cut(s) 36, 239, 569, 905, 1347, 1392
TaiI ACGT 3 cut(s) 76, 398, 1045
TaqI TCGA 4 cut(s) 112, 576, 1022, 1375
TasI AATT 8 cut(s) 14, 135, 401, 523, 543, 556, 747, 879
TatI WGTACW 2 cut(s) 1350, 1392
TfiI GAWTC 5 cut(s) 573, 1129, 1136, 1283, 1320
Tru1I TTAA 7 cut(s) 122, 138, 405, 440, 510, 522, 527
Tru9I TTAA 7 cut(s) 122, 138, 405, 440, 510, 522, 527
TscAI CASTG 2 cut(s) 354, 1264
TseI GCWGC 4 cut(s) 4, 242, 851, 992
TspDTI ATGAA 8 cut(s) 617, 925, 990, 1121, 1124, 1149, 1199, 1208
TspGWI ACGGA 1 cut(s) 548
TspRI CASTG 2 cut(s) 354, 1264
VpaK11BI GGWCC 4 cut(s) 143, 416, 1277, 1370
VspI ATTAAT 1 cut(s) 138
XapI RAATTY 2 cut(s) 556, 747
XceI RCATGY 2 cut(s) 369, 1357
XhoI CTCGAG 1 cut(s) 1374
XmaJI CCTAGG 1 cut(s) 49
XmiI GTMKAC 1 cut(s) 539
XspI CTAG 2 cut(s) 50, 813
ZrmI AGTACT 1 cut(s) 1394
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.