Rh5DG517900

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0001338
Physical Location & Seq
Reverse (-)
80486044 .. 80488657
2614 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG517900.1

Sequence Viewer

Length: 1143 bp
ATGGAGCTCCTCCACGGGTTCGATGTGAAAATGTTTGCGTCGACGGTGGCGCTGATCGGGCTTATGGGGTTGCTGGTGCGGTTGTATAAGGAGCTAGTGGCGGAGCCGAAGAGGCTGAGATCCCTGCTGGCCAAGCAAGGCATCACGGGACCTCCACAGGCTTTGCTTCATGGGAATATCTCGGAGATAAACAAGGCTCGCCGCGGCGGCGGCATTGCTAAGGCTCCGAGCAGTGAACCACCCACCTCACACAACTTGGCTGCCACTATCTACCCCTACTTTGATAAGTGGAGGAAGCAATTTGGTGATGTGTTTGTGATTGGTCATGGCACCACGCAAATACTATATGTGATCAAACCAGAAGCAGTGAGGGAGATCACAACCTGCATATCCTTGGACTTGGGGAAACCCACATACCAATTCAAACAGCGTGGTCCTTTACTTGGTAAAGGTATTTTGACCTCCAATGGAGCCTCGTGGGCTTATCAGCGCAAGGTCATGGCTCCTGAACTTTACATGGAGAAGGTCAAGGGAATGTTCAACTTAATTACCGAATCCTCGAATACTCTGGTGAACTTGTGGAACAGTAAGATTGATGCAGGCAAAGGAGTTGCAGACATAAAAATCGGCAACGATTTGAGATGCTTCTCTGGTGATGTTATCTCGAGGGCATGTTTTGGGAGCAGTTATACCAAAGGGGTACAGATTTTTGATAAACTAAAGCAACTCCAGGACGCCATGGCCAGGAAAGTCTTTGCGGTTGCAGTTCCTGGAATGAGACACCTTCCTACAAAGAGCAATAGGGAAGAATGGGCATTAGAAAAGGAGGTTGCGAGGTTGATACTGCAATTAGTGAAGGAGAGACAGGCAGCTGGGCACAAAAAAGACTTATTGCAAATGATTCTTGAGGGTGCAAAAGCCAGTGATCTCAGTCAAGATGCAGCAGACAGCTTCATTGTTGATAACTGCAAGAACATATACTTGGCTGGATATGAAACCACAGCTACTTCTACCTCATGGTGCCTCATGCTGTTGGCTTCGAATCAAGAATGGCAAGAACGCGTTAGAGCCGAGGTCCTTCAAGTTTGCCAAGGCCGCGTTCCAGATACGGATATGCTTCGCAAAATGAAACAGTTAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

380

Amino Acids

42.22

Weight (kDa)

9.42

Isoelectric Point (pI)

38.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 90 - 378 4.6e-36 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 392
AccB1I GGYRCC 2 cut(s) 329, 1020
AccI GTMKAC 1 cut(s) 41
AccII CGCG 3 cut(s) 204, 1062, 1098
AciI CCGC 8 cut(s) 79, 101, 202, 204, 207, 210, 758, 1096
AclWI GGATC 1 cut(s) 114
AcoI YGGCCR 2 cut(s) 129, 741
AcyI GRCGYC 1 cut(s) 735
AfaI GTAC 1 cut(s) 702
AfiI CCNNNNNNNGG 2 cut(s) 443, 744
AflIII ACRYGT 1 cut(s) 1060
AgsI TTSAA 3 cut(s) 424, 541, 1082
AjnI CCWGG 3 cut(s) 729, 743, 769
AjuI GAANNNNNNNTTGG 4 cut(s) 965, 997, 1083, 1115
AluBI AGCT 5 cut(s) 7, 94, 872, 951, 1004
AluI AGCT 5 cut(s) 7, 94, 872, 951, 1004
Alw21I GWGCWC 1 cut(s) 9
Alw26I GTCTC 2 cut(s) 772, 856
AlwI GGATC 1 cut(s) 114
AlwNI CAGNNNCTG 1 cut(s) 770
Ama87I CYCGRG 1 cut(s) 664
AoxI GGCC 3 cut(s) 129, 741, 1093
ApeKI GCWGC 3 cut(s) 260, 869, 941
AspLEI GCGC 2 cut(s) 52, 492
AspS9I GGNCC 3 cut(s) 149, 434, 1075
AsuHPI GGTGA 3 cut(s) 317, 583, 665
AsuII TTCGAA 1 cut(s) 1040
AvaI CYCGRG 1 cut(s) 664
AvaII GGWCC 3 cut(s) 149, 434, 1075
BaeGI GKGCMC 1 cut(s) 879
BalI TGGCCA 2 cut(s) 131, 743
BanI GGYRCC 2 cut(s) 329, 1020
BanII GRGCYC 1 cut(s) 9
BauI CACGAG 1 cut(s) 475
Bbv12I GWGCWC 1 cut(s) 9
BbvI GCAGC 3 cut(s) 247, 881, 953
BciT130I CCWGG 3 cut(s) 731, 745, 771
BclI TGATCA 1 cut(s) 351
BcoDI GTCTC 2 cut(s) 772, 856
BfaI CTAG 1 cut(s) 95
BfoI RGCGCY 1 cut(s) 53
BfuAI ACCTGC 1 cut(s) 392
BglI GCCNNNNNGGC 3 cut(s) 112, 207, 479
BisI GCNGC 8 cut(s) 202, 205, 208, 211, 261, 870, 942, 1096
BlsI GCNGC 8 cut(s) 203, 206, 209, 212, 262, 871, 943, 1097
Bme1390I CCNGG 3 cut(s) 731, 745, 771
Bme18I GGWCC 3 cut(s) 149, 434, 1075
BmeT110I CYCGRG 1 cut(s) 664
BmgT120I GGNCC 3 cut(s) 149, 434, 1075
BmiI GGNNCC 7 cut(s) 105, 150, 225, 331, 472, 504, 1022
BmrFI CCNGG 3 cut(s) 731, 745, 771
BmsI GCATC 4 cut(s) 150, 586, 632, 928
BpmI CTGGAG 1 cut(s) 713
Bpu10I CCTNAGC 1 cut(s) 219
Bpu14I TTCGAA 1 cut(s) 1040
BpuEI CTTGAG 1 cut(s) 926
BsaHI GRCGYC 1 cut(s) 735
BsaJI CCNNGG 6 cut(s) 13, 202, 393, 738, 1071, 1090
BsaXI ACNNNNNCTCC 2 cut(s) 136, 166
Bsc4I CCNNNNNNNGG 2 cut(s) 443, 744
Bse1I ACTGG 1 cut(s) 921
Bse3DI GCAATG 1 cut(s) 213
BseBI CCWGG 3 cut(s) 731, 745, 771
BseDI CCNNGG 6 cut(s) 13, 202, 393, 738, 1071, 1090
BseLI CCNNNNNNNGG 2 cut(s) 443, 744
BseMI GCAATG 1 cut(s) 213
BseMII CTCAG 2 cut(s) 107, 943
BseNI ACTGG 1 cut(s) 921
BseSI GKGCMC 1 cut(s) 879
BseXI GCAGC 3 cut(s) 247, 881, 953
BseYI CCCAGC 1 cut(s) 872
Bsh1236I CGCG 3 cut(s) 204, 1062, 1098
BshFI GGCC 3 cut(s) 131, 743, 1095
BshNI GGYRCC 2 cut(s) 329, 1020
BsiHKAI GWGCWC 1 cut(s) 9
BsiHKCI CYCGRG 1 cut(s) 664
BslFI GGGAC 1 cut(s) 162
BslI CCNNNNNNNGG 2 cut(s) 443, 744
BsmAI GTCTC 2 cut(s) 772, 856
BsmFI GGGAC 1 cut(s) 162
BsnI GGCC 3 cut(s) 131, 743, 1095
BsoBI CYCGRG 1 cut(s) 664
Bsp119I TTCGAA 1 cut(s) 1040
Bsp1286I GDGCHC 2 cut(s) 9, 879
Bsp143I GATC 5 cut(s) 54, 119, 351, 375, 925
Bsp19I CCATGG 1 cut(s) 738
BspACI CCGC 8 cut(s) 79, 101, 202, 204, 207, 210, 758, 1096
BspANI GGCC 3 cut(s) 131, 743, 1095
BspCNI CTCAG 2 cut(s) 108, 942
BspFNI CGCG 3 cut(s) 204, 1062, 1098
BspLI GGNNCC 7 cut(s) 105, 150, 225, 331, 472, 504, 1022
BspMI ACCTGC 1 cut(s) 392
BspPI GGATC 1 cut(s) 114
BspT104I TTCGAA 1 cut(s) 1040
BspT107I GGYRCC 2 cut(s) 329, 1020
BsrDI GCAATG 1 cut(s) 213
BsrI ACTGG 1 cut(s) 921
BssECI CCNNGG 6 cut(s) 13, 202, 393, 738, 1071, 1090
BssMI GATC 5 cut(s) 54, 119, 351, 375, 925
BssNI GRCGYC 1 cut(s) 735
BssSI CACGAG 1 cut(s) 475
BssT1I CCWWGG 3 cut(s) 393, 738, 1090
Bst2BI CACGAG 1 cut(s) 475
Bst2UI CCWGG 3 cut(s) 731, 745, 771
Bst4CI ACNGT 3 cut(s) 46, 587, 1134
Bst6I CTCTTC 1 cut(s) 104
BstACI GRCGYC 1 cut(s) 735
BstBI TTCGAA 1 cut(s) 1040
BstC8I GCNNGC 3 cut(s) 129, 199, 601
BstDEI CTNAG 3 cut(s) 116, 219, 929
BstDSI CCRYGG 3 cut(s) 13, 202, 738
BstFNI CGCG 3 cut(s) 204, 1062, 1098
BstH2I RGCGCY 1 cut(s) 53
BstHHI GCGC 2 cut(s) 52, 492
BstKTI GATC 5 cut(s) 57, 122, 354, 378, 928
BstMAI GTCTC 2 cut(s) 772, 856
BstMBI GATC 5 cut(s) 54, 119, 351, 375, 925
BstMWI GCNNNNNNNGC 7 cut(s) 58, 112, 133, 207, 210, 479, 1095
BstNI CCWGG 3 cut(s) 731, 745, 771
BstNSI RCATGY 1 cut(s) 675
BstSCI CCNGG 3 cut(s) 729, 743, 769
BstSLI GKGCMC 1 cut(s) 879
BstUI CGCG 3 cut(s) 204, 1062, 1098
BstV1I GCAGC 3 cut(s) 247, 881, 953
BstX2I RGATCY 1 cut(s) 119
BstYI RGATCY 1 cut(s) 119
BsuRI GGCC 3 cut(s) 131, 743, 1095
BtgI CCRYGG 3 cut(s) 13, 202, 738
BtsI GCAGTG 2 cut(s) 238, 372
BtsIMutI CAGTG 3 cut(s) 238, 372, 928
BveI ACCTGC 1 cut(s) 392
Cac8I GCNNGC 3 cut(s) 129, 199, 601
CaiI CAGNNNCTG 1 cut(s) 770
CfoI GCGC 2 cut(s) 52, 492
Cfr13I GGNCC 3 cut(s) 149, 434, 1075
Cfr42I CCGCGG 1 cut(s) 205
CseI GACGC 2 cut(s) 27, 743
Csp6I GTAC 1 cut(s) 701
CspCI CAANNNNNGTGG 4 cut(s) 144, 179, 412, 447
CviAII CATG 9 cut(s) 170, 326, 499, 517, 672, 739, 1017, 1027, 1140
CviQI GTAC 1 cut(s) 701
DdeI CTNAG 3 cut(s) 116, 219, 929
DpnI GATC 5 cut(s) 56, 121, 353, 377, 927
DpnII GATC 5 cut(s) 54, 119, 351, 375, 925
EaeI YGGCCR 2 cut(s) 129, 741
Eam1104I CTCTTC 1 cut(s) 104
EarI CTCTTC 1 cut(s) 104
EciI GGCGGA 1 cut(s) 116
Ecl136II GAGCTC 1 cut(s) 7
Eco130I CCWWGG 3 cut(s) 393, 738, 1090
Eco24I GRGCYC 1 cut(s) 9
Eco47I GGWCC 3 cut(s) 149, 434, 1075
Eco53kI GAGCTC 1 cut(s) 7
Eco88I CYCGRG 1 cut(s) 664
EcoICRI GAGCTC 1 cut(s) 7
EcoO109I RGGNCCY 2 cut(s) 149, 1075
EcoRII CCWGG 3 cut(s) 729, 743, 769
EcoT14I CCWWGG 3 cut(s) 393, 738, 1090
EcoT38I GRGCYC 1 cut(s) 9
ErhI CCWWGG 3 cut(s) 393, 738, 1090
FaeI CATG 9 cut(s) 173, 329, 502, 520, 675, 742, 1020, 1030, 1143
FaqI GGGAC 1 cut(s) 162
FatI CATG 9 cut(s) 169, 325, 498, 516, 671, 738, 1016, 1026, 1139
FbaI TGATCA 1 cut(s) 351
FblI GTMKAC 1 cut(s) 41
Fnu4HI GCNGC 8 cut(s) 202, 205, 208, 211, 261, 870, 942, 1096
FriOI GRGCYC 1 cut(s) 9
Fsp4HI GCNGC 8 cut(s) 202, 205, 208, 211, 261, 870, 942, 1096
FspBI CTAG 1 cut(s) 95
GlaI GCGC 2 cut(s) 51, 491
GluI GCNGC 8 cut(s) 202, 205, 208, 211, 261, 870, 942, 1096
GsaI CCCAGC 1 cut(s) 876
GsuI CTGGAG 1 cut(s) 713
HaeII RGCGCY 1 cut(s) 53
HaeIII GGCC 3 cut(s) 131, 743, 1095
HgaI GACGC 2 cut(s) 27, 743
HhaI GCGC 2 cut(s) 52, 492
Hin1I GRCGYC 1 cut(s) 735
Hin1II CATG 9 cut(s) 173, 329, 502, 520, 675, 742, 1020, 1030, 1143
Hin6I GCGC 2 cut(s) 50, 490
HinP1I GCGC 2 cut(s) 50, 490
HincII GTYRAC 2 cut(s) 42, 1137
HindII GTYRAC 2 cut(s) 42, 1137
HinfI GANTC 3 cut(s) 554, 901, 1042
HpaI GTTAAC 1 cut(s) 1137
HphI GGTGA 3 cut(s) 317, 583, 665
Hpy166II GTNNAC 4 cut(s) 42, 236, 574, 1137
Hpy188I TCNGA 2 cut(s) 184, 228
Hpy188III TCNNGA 6 cut(s) 506, 664, 905, 935, 1046, 1103
Hpy8I GTNNAC 4 cut(s) 42, 236, 574, 1137
Hpy99I CGWCG 2 cut(s) 43, 46
HpyAV CCTTC 4 cut(s) 517, 794, 850, 1088
HpyCH4III ACNGT 3 cut(s) 46, 587, 1134
HpyCH4V TGCA 9 cut(s) 387, 599, 614, 764, 847, 895, 914, 941, 969
HpyF10VI GCNNNNNNNGC 7 cut(s) 58, 112, 133, 207, 210, 479, 1095
HpyF3I CTNAG 3 cut(s) 116, 219, 929
Hsp92I GRCGYC 1 cut(s) 735
Hsp92II CATG 9 cut(s) 173, 329, 502, 520, 675, 742, 1020, 1030, 1143
HspAI GCGC 2 cut(s) 50, 490
Ksp22I TGATCA 1 cut(s) 351
KspAI GTTAAC 1 cut(s) 1137
KspI CCGCGG 1 cut(s) 205
Kzo9I GATC 5 cut(s) 54, 119, 351, 375, 925
LmnI GCTCC 8 cut(s) 4, 12, 91, 103, 229, 470, 508, 681
Lsp1109I GCAGC 3 cut(s) 247, 881, 953
LweI GCATC 4 cut(s) 150, 586, 632, 928
MaeI CTAG 1 cut(s) 95
MalI GATC 5 cut(s) 56, 121, 353, 377, 927
MboI GATC 5 cut(s) 54, 119, 351, 375, 925
MboII GAAGA 2 cut(s) 121, 818
MflI RGATCY 1 cut(s) 119
MhlI GDGCHC 2 cut(s) 9, 879
MlsI TGGCCA 2 cut(s) 131, 743
MluCI AATT 4 cut(s) 299, 419, 546, 848
MluI ACGCGT 1 cut(s) 1060
MluNI TGGCCA 2 cut(s) 131, 743
Mox20I TGGCCA 2 cut(s) 131, 743
MscI TGGCCA 2 cut(s) 131, 743
MseI TTAA 2 cut(s) 545, 1136
Msp20I TGGCCA 2 cut(s) 131, 743
MspA1I CMGCKG 2 cut(s) 204, 872
MspR9I CCNGG 3 cut(s) 731, 745, 771
MvaI CCWGG 3 cut(s) 731, 745, 771
MvnI CGCG 3 cut(s) 204, 1062, 1098
MwoI GCNNNNNNNGC 7 cut(s) 58, 112, 133, 207, 210, 479, 1095
NcoI CCATGG 1 cut(s) 738
NdeII GATC 5 cut(s) 54, 119, 351, 375, 925
NlaIII CATG 9 cut(s) 173, 329, 502, 520, 675, 742, 1020, 1030, 1143
NlaIV GGNNCC 7 cut(s) 105, 150, 225, 331, 472, 504, 1022
NmeAIII GCCGAG 1 cut(s) 1096
NspI RCATGY 1 cut(s) 675
NspV TTCGAA 1 cut(s) 1040
PaeR7I CTCGAG 1 cut(s) 664
PfeI GAWTC 3 cut(s) 554, 901, 1042
PfoI TCCNGGA 2 cut(s) 729, 769
PkrI GCNGC 8 cut(s) 203, 206, 209, 212, 262, 871, 943, 1097
PpuMI RGGWCCY 2 cut(s) 149, 1075
Psp124BI GAGCTC 1 cut(s) 9
Psp5II RGGWCCY 2 cut(s) 149, 1075
Psp6I CCWGG 3 cut(s) 729, 743, 769
PspFI CCCAGC 1 cut(s) 872
PspGI CCWGG 3 cut(s) 729, 743, 769
PspN4I GGNNCC 7 cut(s) 105, 150, 225, 331, 472, 504, 1022
PspPI GGNCC 3 cut(s) 149, 434, 1075
PspPPI RGGWCCY 2 cut(s) 149, 1075
PstNI CAGNNNCTG 1 cut(s) 770
PsuI RGATCY 1 cut(s) 119
PvuII CAGCTG 1 cut(s) 872
RsaI GTAC 1 cut(s) 702
RsaNI GTAC 1 cut(s) 701
SacI GAGCTC 1 cut(s) 9
SacII CCGCGG 1 cut(s) 205
SalI GTCGAC 1 cut(s) 40
SaqAI TTAA 2 cut(s) 545, 1136
SatI GCNGC 8 cut(s) 202, 205, 208, 211, 261, 870, 942, 1096
Sau3AI GATC 5 cut(s) 54, 119, 351, 375, 925
Sau96I GGNCC 3 cut(s) 149, 434, 1075
ScrFI CCNGG 3 cut(s) 731, 745, 771
SduI GDGCHC 2 cut(s) 9, 879
SfaNI GCATC 4 cut(s) 150, 586, 632, 928
Sfr274I CTCGAG 1 cut(s) 664
Sfr303I CCGCGG 1 cut(s) 205
SfuI TTCGAA 1 cut(s) 1040
SgrBI CCGCGG 1 cut(s) 205
SgrDI CGTCGACG 1 cut(s) 40
SinI GGWCC 3 cut(s) 149, 434, 1075
SlaI CTCGAG 1 cut(s) 664
SmlI CTYRAG 2 cut(s) 664, 905
SmoI CTYRAG 2 cut(s) 664, 905
Sse9I AATT 4 cut(s) 299, 419, 546, 848
SsiI CCGC 8 cut(s) 79, 101, 202, 204, 207, 210, 758, 1096
SspMI CTAG 1 cut(s) 95
SstI GAGCTC 1 cut(s) 9
StyD4I CCNGG 3 cut(s) 729, 743, 769
StyI CCWWGG 3 cut(s) 393, 738, 1090
TaaI ACNGT 3 cut(s) 46, 587, 1134
TaqI TCGA 5 cut(s) 21, 41, 560, 665, 1040
TasI AATT 4 cut(s) 299, 419, 546, 848
TauI GCSGC 5 cut(s) 204, 207, 210, 213, 1098
TfiI GAWTC 3 cut(s) 554, 901, 1042
Tru1I TTAA 2 cut(s) 545, 1136
Tru9I TTAA 2 cut(s) 545, 1136
TscAI CASTG 3 cut(s) 238, 372, 928
TseI GCWGC 3 cut(s) 260, 869, 941
TspDTI ATGAA 4 cut(s) 158, 943, 1008, 1142
TspGWI ACGGA 1 cut(s) 1124
TspRI CASTG 3 cut(s) 238, 372, 928
VpaK11BI GGWCC 3 cut(s) 149, 434, 1075
XceI RCATGY 1 cut(s) 675
XhoI CTCGAG 1 cut(s) 664
XmiI GTMKAC 1 cut(s) 41
XspI CTAG 1 cut(s) 95
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.