pycom03g03700

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0001338
Physical Location & Seq
Forward (+)
2941214 .. 2944465
3252 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g03700.6

Sequence Viewer

Length: 1548 bp
ATGCAAGGCAGTCTGCTAGGGTTTCCCAGCTGCATGAATAACATAATTGCATCAATGCAGCTCCTCCAATTTGATGCAAAAGTGTTACTGTCCCTTGTGTTGCTAGGGTTTATAGGATTGGTGGCAGGTCTCTACAACGGGCTTGTTGGACCTCCACATACTATTCTCCTGGGAAATATTTTGGAAATAAAGAAGGCTCGAGGCTCCAGCAATCCTACAAGTGAATCCCCGACCTCTCACAACTGTGCTGCTCTTCTCTTTCCATTCTTTGAGAAGTGGAGGAAGCTATATGGTGAAGTATTTGTGTTTGCACTTGGAAACACACAAATATTATATGTGAACCAGCCTGACGCAGTGAGGGACATTACAACGTGCACATCATTGGACTTGGGGAAACCAACGTATCAATTTAAAGAGCGCGGTCCTTTGCTTGGTCAAGGCATTTTAACCTCAAATGGCACCTCGTGGGTGCATCAGCGCAAAATCATTGCTCCTGAACTATACATGGAGAAAGTTAAGGGAATGGTTAACTTAATCACGGAGTCCACAATTACTCTGATAAATTCGTGGAACAGTAGAATCGAGGCAGAGGGAGGAATTTCTGACATGAAAATAGACAGCTACATTGGAAGCTTCTCTGGCGATGCTATCTCAAGAGCTTGTTTTGGAAGCAACTATTCCAAAGGGGAGGAGATATTTCAGAAACTAAAGCAAATCCAGGAGGCAATGGGGAGGAAAGCTTTTCTGGCCGGAATTCCTGGAATGAGGCACCTTCCCACTAAGAGCAATAGGGAAGTTTGGGCCTTCGAAAAGGAGGCTAGCGCTTTGATACTGCAGGTAGTGAAGGAAAGGAGGGCAGCAGAAAATGAGAAAGACTTATTGCAAATTATTCTTGAGAGTGCTAAAAACAGTAACCTTAGCCAAGAGGCAACAGACCGCTTCATCGTTGATAACTGCAAGAACATATACTTGGCCGGGTATGAAACCACAGCGGTCGCAGCCACATGGTGCCTCATGCTGTTGGCTTCGAACCAAGAATGGCAAGAACGTGTGAGAGCAGAGGCCCTTCAAGTTTGCCAGGGCCACATTCCAGATGCTGATATGGTTCGCAACATGAAACAGCTAACAATGGTGATTCATGAATCATTGCGCCTATATCCCCCGGTTAGTGTGGTGTCAAGGGAGGCTTTCAAGGACATGAAGTTCGGGGAGATTCATGTTCCAAAGGGTATCAATGTTTGGACAATGGTGATAACATTGCACACTGATCCGAAACTATGGGGACAAGATTCGTATGCTTTCAACCCCAATAGGTTTGCAAATGGAATCACAGGTGCTTGCAAGCTTCCACAGTTGTACATGCCATTTGGAGTTGGTCCTCGAGTGTGTCTTGGACAGTACTTTGCCATGGTTGAACTCAAGGTTCTGATAGCTCTCATTGTATCCAACTTCTCTTTCTCACTTTCTCCCAAGTACCGCCATGCGCCTACTCTTAGATTGGTCTTAGAGCCGGAACATGGTGTTGATCTCTTGGTGAAGAAGTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

516

Amino Acids

57.37

Weight (kDa)

8.69

Isoelectric Point (pI)

34.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 116, 826
AccB1I GGYRCC 3 cut(s) 458, 768, 1008
AccII CGCG 1 cut(s) 420
AciI CCGC 4 cut(s) 420, 937, 992, 1477
AclWI GGATC 1 cut(s) 1262
AcoI YGGCCR 2 cut(s) 747, 972
AcsI RAATTY 3 cut(s) 562, 597, 753
AdeI CACNNNGTG 2 cut(s) 465, 1008
AfaI GTAC 3 cut(s) 1358, 1400, 1475
AfeI AGCGCT 1 cut(s) 823
AfiI CCNNNNNNNGG 2 cut(s) 431, 1517
AflIII ACRYGT 1 cut(s) 1048
AgsI TTSAA 4 cut(s) 1070, 1192, 1303, 1415
AjnI CCWGG 4 cut(s) 168, 717, 757, 1077
AjuI GAANNNNNNNTTGG 4 cut(s) 953, 985, 1439, 1471
AleI CACNNNNGTG 1 cut(s) 243
AloI GAACNNNNNNTCC 2 cut(s) 1187, 1219
Alw21I GWGCWC 1 cut(s) 377
Alw26I GTCTC 1 cut(s) 134
Alw44I GTGCAC 1 cut(s) 373
AlwI GGATC 1 cut(s) 1262
AlwNI CAGNNNCTG 1 cut(s) 1097
Ama87I CYCGRG 2 cut(s) 198, 1380
Aor51HI AGCGCT 1 cut(s) 823
AoxI GGCC 5 cut(s) 747, 801, 972, 1062, 1081
ApaLI GTGCAC 1 cut(s) 373
ApeKI GCWGC 5 cut(s) 30, 58, 248, 857, 998
ApoI RAATTY 3 cut(s) 562, 597, 753
ArsI GACNNNNNNTTYG 2 cut(s) 1187, 1219
AspLEI GCGC 5 cut(s) 420, 480, 824, 1152, 1486
AspS9I GGNCC 6 cut(s) 149, 422, 801, 1063, 1081, 1376
AsuC2I CCSGG 2 cut(s) 976, 1163
AsuHPI GGTGA 4 cut(s) 305, 1144, 1261, 1546
AsuII TTCGAA 2 cut(s) 807, 1028
AsuNHI GCTAGC 1 cut(s) 818
AvaI CYCGRG 2 cut(s) 198, 1380
AvaII GGWCC 3 cut(s) 149, 422, 1376
BaeGI GKGCMC 1 cut(s) 377
BaeI ACNNNNGTAYC 2 cut(s) 386, 419
BanI GGYRCC 3 cut(s) 458, 768, 1008
BauI CACGAG 1 cut(s) 463
Bbv12I GWGCWC 1 cut(s) 377
BbvI GCAGC 5 cut(s) 17, 70, 235, 869, 1010
BciT130I CCWGG 4 cut(s) 170, 719, 759, 1079
BciVI GTATCC 1 cut(s) 1453
BcnI CCSGG 2 cut(s) 976, 1163
BcoDI GTCTC 1 cut(s) 134
BfaI CTAG 3 cut(s) 17, 104, 819
BfmI CTRYAG 1 cut(s) 833
BfoI RGCGCY 1 cut(s) 825
BfuAI ACCTGC 2 cut(s) 116, 826
BfuI GTATCC 1 cut(s) 1453
BisI GCNGC 5 cut(s) 31, 59, 249, 858, 999
BlsI GCNGC 5 cut(s) 32, 60, 250, 859, 1000
BmcAI AGTACT 1 cut(s) 1400
Bme1390I CCNGG 6 cut(s) 170, 719, 759, 976, 1079, 1163
Bme18I GGWCC 3 cut(s) 149, 422, 1376
BmeT110I CYCGRG 2 cut(s) 198, 1380
BmgT120I GGNCC 6 cut(s) 149, 422, 801, 1063, 1081, 1376
BmiI GGNNCC 4 cut(s) 205, 460, 770, 1010
BmrFI CCNGG 6 cut(s) 170, 719, 759, 976, 1079, 1163
BmsI GCATC 5 cut(s) 59, 64, 481, 634, 1084
BmtI GCTAGC 1 cut(s) 822
BpmI CTGGAG 1 cut(s) 190
Bpu10I CCTNAGC 1 cut(s) 917
Bpu14I TTCGAA 2 cut(s) 807, 1028
BpuEI CTTGAG 3 cut(s) 637, 914, 1403
BpuMI CCSGG 2 cut(s) 976, 1163
BsaI GGTCTC 1 cut(s) 134
BsaJI CCNNGG 4 cut(s) 169, 1078, 1161, 1407
Bsc4I CCNNNNNNNGG 2 cut(s) 431, 1517
Bse3DI GCAATG 4 cut(s) 486, 732, 1145, 1256
BseBI CCWGG 4 cut(s) 170, 719, 759, 1079
BseDI CCNNGG 4 cut(s) 169, 1078, 1161, 1407
BseLI CCNNNNNNNGG 2 cut(s) 431, 1517
BseMI GCAATG 4 cut(s) 486, 732, 1145, 1256
BseRI GAGGAG 2 cut(s) 53, 704
BseSI GKGCMC 1 cut(s) 377
BseXI GCAGC 5 cut(s) 17, 70, 235, 869, 1010
BseYI CCCAGC 1 cut(s) 26
Bsh1236I CGCG 1 cut(s) 420
Bsh1285I CGRYCG 1 cut(s) 996
BshFI GGCC 5 cut(s) 749, 803, 974, 1064, 1083
BshNI GGYRCC 3 cut(s) 458, 768, 1008
BsiEI CGRYCG 1 cut(s) 996
BsiHKAI GWGCWC 1 cut(s) 377
BsiHKCI CYCGRG 2 cut(s) 198, 1380
BsiSI CCGG 4 cut(s) 750, 975, 1163, 1511
BslFI GGGAC 3 cut(s) 76, 374, 1296
BslI CCNNNNNNNGG 2 cut(s) 431, 1517
BsmAI GTCTC 1 cut(s) 134
BsmFI GGGAC 3 cut(s) 76, 374, 1296
BsnI GGCC 5 cut(s) 749, 803, 974, 1064, 1083
Bso31I GGTCTC 1 cut(s) 134
BsoBI CYCGRG 2 cut(s) 198, 1380
Bsp119I TTCGAA 2 cut(s) 807, 1028
Bsp1286I GDGCHC 1 cut(s) 377
Bsp1407I TGTACA 1 cut(s) 1356
Bsp143I GATC 2 cut(s) 1267, 1525
Bsp19I CCATGG 1 cut(s) 1407
BspACI CCGC 4 cut(s) 420, 937, 992, 1477
BspANI GGCC 5 cut(s) 749, 803, 974, 1064, 1083
BspFNI CGCG 1 cut(s) 420
BspHI TCATGA 1 cut(s) 1138
BspLI GGNNCC 4 cut(s) 205, 460, 770, 1010
BspMAI CTGCAG 1 cut(s) 837
BspMI ACCTGC 2 cut(s) 116, 826
BspOI GCTAGC 1 cut(s) 822
BspPI GGATC 1 cut(s) 1262
BspQI GCTCTTC 1 cut(s) 258
BspT104I TTCGAA 2 cut(s) 807, 1028
BspT107I GGYRCC 3 cut(s) 458, 768, 1008
BspTNI GGTCTC 1 cut(s) 134
BsrDI GCAATG 4 cut(s) 486, 732, 1145, 1256
BsrGI TGTACA 1 cut(s) 1356
BssECI CCNNGG 4 cut(s) 169, 1078, 1161, 1407
BssMI GATC 2 cut(s) 1267, 1525
BssSI CACGAG 1 cut(s) 463
BssT1I CCWWGG 1 cut(s) 1407
Bst2BI CACGAG 1 cut(s) 463
Bst2UI CCWGG 4 cut(s) 170, 719, 759, 1079
Bst4CI ACNGT 6 cut(s) 90, 245, 575, 911, 1353, 1398
Bst6I CTCTTC 1 cut(s) 258
BstAUI TGTACA 1 cut(s) 1356
BstBI TTCGAA 2 cut(s) 807, 1028
BstC8I GCNNGC 3 cut(s) 820, 1339, 1343
BstDEI CTNAG 4 cut(s) 780, 917, 1493, 1504
BstDSI CCRYGG 1 cut(s) 1407
BstFNI CGCG 1 cut(s) 420
BstH2I RGCGCY 1 cut(s) 825
BstHHI GCGC 5 cut(s) 420, 480, 824, 1152, 1486
BstKTI GATC 2 cut(s) 1270, 1528
BstMAI GTCTC 1 cut(s) 134
BstMBI GATC 2 cut(s) 1267, 1525
BstMCI CGRYCG 1 cut(s) 996
BstMWI GCNNNNNNNGC 3 cut(s) 639, 746, 998
BstNI CCWGG 4 cut(s) 170, 719, 759, 1079
BstNSI RCATGY 1 cut(s) 1363
BstSCI CCNGG 6 cut(s) 168, 717, 757, 974, 1077, 1161
BstSFI CTRYAG 1 cut(s) 833
BstSLI GKGCMC 1 cut(s) 377
BstUI CGCG 1 cut(s) 420
BstV1I GCAGC 5 cut(s) 17, 70, 235, 869, 1010
BsuI GTATCC 1 cut(s) 1453
BsuRI GGCC 5 cut(s) 749, 803, 974, 1064, 1083
BtgI CCRYGG 1 cut(s) 1407
BtgZI GCGATG 1 cut(s) 657
BtsI GCAGTG 1 cut(s) 360
BtsIMutI CAGTG 2 cut(s) 360, 1263
BveI ACCTGC 2 cut(s) 116, 826
Cac8I GCNNGC 3 cut(s) 820, 1339, 1343
CaiI CAGNNNCTG 1 cut(s) 1097
CciI TCATGA 1 cut(s) 1138
CfoI GCGC 5 cut(s) 420, 480, 824, 1152, 1486
Cfr13I GGNCC 6 cut(s) 149, 422, 801, 1063, 1081, 1376
CseI GACGC 1 cut(s) 359
Csp6I GTAC 3 cut(s) 1357, 1399, 1474
CviQI GTAC 3 cut(s) 1357, 1399, 1474
DdeI CTNAG 4 cut(s) 780, 917, 1493, 1504
DpnI GATC 2 cut(s) 1269, 1527
DpnII GATC 2 cut(s) 1267, 1525
DraI TTTAAA 1 cut(s) 412
DraIII CACNNNGTG 2 cut(s) 465, 1008
EaeI YGGCCR 2 cut(s) 747, 972
Eam1104I CTCTTC 1 cut(s) 258
EarI CTCTTC 1 cut(s) 258
Eco130I CCWWGG 1 cut(s) 1407
Eco31I GGTCTC 1 cut(s) 134
Eco47I GGWCC 3 cut(s) 149, 422, 1376
Eco47III AGCGCT 1 cut(s) 823
Eco88I CYCGRG 2 cut(s) 198, 1380
EcoO109I RGGNCCY 1 cut(s) 1063
EcoRI GAATTC 1 cut(s) 753
EcoRII CCWGG 4 cut(s) 168, 717, 757, 1077
EcoT14I CCWWGG 1 cut(s) 1407
ErhI CCWWGG 1 cut(s) 1407
FalI AAGNNNNNCTT 2 cut(s) 1171, 1203
FaqI GGGAC 3 cut(s) 76, 374, 1296
Fnu4HI GCNGC 5 cut(s) 31, 59, 249, 858, 999
Fsp4HI GCNGC 5 cut(s) 31, 59, 249, 858, 999
FspBI CTAG 3 cut(s) 17, 104, 819
GlaI GCGC 5 cut(s) 419, 479, 823, 1151, 1485
GluI GCNGC 5 cut(s) 31, 59, 249, 858, 999
GsaI CCCAGC 1 cut(s) 30
GsuI CTGGAG 1 cut(s) 190
HaeII RGCGCY 1 cut(s) 825
HaeIII GGCC 5 cut(s) 749, 803, 974, 1064, 1083
HapII CCGG 4 cut(s) 750, 975, 1163, 1511
HgaI GACGC 1 cut(s) 359
HhaI GCGC 5 cut(s) 420, 480, 824, 1152, 1486
Hin6I GCGC 5 cut(s) 418, 478, 822, 1150, 1484
HinP1I GCGC 5 cut(s) 418, 478, 822, 1150, 1484
HincII GTYRAC 1 cut(s) 529
HindII GTYRAC 1 cut(s) 529
HindIII AAGCTT 3 cut(s) 631, 738, 1343
HinfI GANTC 8 cut(s) 224, 542, 579, 1135, 1142, 1213, 1289, 1326
HpaI GTTAAC 1 cut(s) 529
HpaII CCGG 4 cut(s) 750, 975, 1163, 1511
HphI GGTGA 4 cut(s) 305, 1144, 1261, 1546
Hpy166II GTNNAC 4 cut(s) 340, 375, 529, 546
Hpy188I TCNGA 5 cut(s) 558, 604, 702, 1272, 1428
Hpy188III TCNNGA 5 cut(s) 494, 654, 893, 1091, 1139
Hpy8I GTNNAC 4 cut(s) 340, 375, 529, 546
HpyAV CCTTC 5 cut(s) 187, 782, 814, 838, 1076
HpyCH4III ACNGT 6 cut(s) 90, 245, 575, 911, 1353, 1398
HpyCH4IV ACGT 3 cut(s) 371, 401, 1048
HpyF10VI GCNNNNNNNGC 3 cut(s) 639, 746, 998
HpyF3I CTNAG 4 cut(s) 780, 917, 1493, 1504
HpySE526I ACGT 3 cut(s) 371, 401, 1048
HspAI GCGC 5 cut(s) 418, 478, 822, 1150, 1484
KspAI GTTAAC 1 cut(s) 529
Kzo9I GATC 2 cut(s) 1267, 1525
LguI GCTCTTC 1 cut(s) 258
LmnI GCTCC 3 cut(s) 66, 209, 496
Lsp1109I GCAGC 5 cut(s) 17, 70, 235, 869, 1010
LweI GCATC 5 cut(s) 59, 64, 481, 634, 1084
MaeI CTAG 3 cut(s) 17, 104, 819
MaeII ACGT 3 cut(s) 371, 401, 1048
MaeIII GTNAC 2 cut(s) 84, 911
MalI GATC 2 cut(s) 1269, 1527
MboI GATC 2 cut(s) 1267, 1525
MboII GAAGA 1 cut(s) 245
MhlI GDGCHC 1 cut(s) 377
MluCI AATT 8 cut(s) 45, 68, 407, 549, 562, 597, 753, 885
MlyI GAGTC 1 cut(s) 551
MmeI TCCRAC 2 cut(s) 127, 1470
MseI TTAA 5 cut(s) 411, 446, 516, 528, 533
MslI CAYNNNNRTG 1 cut(s) 243
MspA1I CMGCKG 2 cut(s) 30, 992
MspI CCGG 4 cut(s) 750, 975, 1163, 1511
MspR9I CCNGG 6 cut(s) 170, 719, 759, 976, 1079, 1163
MvaI CCWGG 4 cut(s) 170, 719, 759, 1079
MvnI CGCG 1 cut(s) 420
MwoI GCNNNNNNNGC 3 cut(s) 639, 746, 998
NciI CCSGG 2 cut(s) 976, 1163
NcoI CCATGG 1 cut(s) 1407
NdeII GATC 2 cut(s) 1267, 1525
NheI GCTAGC 1 cut(s) 818
NlaIV GGNNCC 4 cut(s) 205, 460, 770, 1010
NspI RCATGY 1 cut(s) 1363
NspV TTCGAA 2 cut(s) 807, 1028
OliI CACNNNNGTG 1 cut(s) 243
PaeR7I CTCGAG 2 cut(s) 198, 1380
PagI TCATGA 1 cut(s) 1138
PciSI GCTCTTC 1 cut(s) 258
PfeI GAWTC 7 cut(s) 224, 579, 1135, 1142, 1213, 1289, 1326
PfoI TCCNGGA 2 cut(s) 717, 757
PkrI GCNGC 5 cut(s) 32, 60, 250, 859, 1000
PleI GAGTC 1 cut(s) 550
PpsI GAGTC 1 cut(s) 550
Psp6I CCWGG 4 cut(s) 168, 717, 757, 1077
PspFI CCCAGC 1 cut(s) 26
PspGI CCWGG 4 cut(s) 168, 717, 757, 1077
PspN4I GGNNCC 4 cut(s) 205, 460, 770, 1010
PspPI GGNCC 6 cut(s) 149, 422, 801, 1063, 1081, 1376
PspXI VCTCGAGB 2 cut(s) 198, 1380
PstI CTGCAG 1 cut(s) 837
PstNI CAGNNNCTG 1 cut(s) 1097
PvuII CAGCTG 1 cut(s) 30
RsaI GTAC 3 cut(s) 1358, 1400, 1475
RsaNI GTAC 3 cut(s) 1357, 1399, 1474
RseI CAYNNNNRTG 1 cut(s) 243
SapI GCTCTTC 1 cut(s) 258
SaqAI TTAA 5 cut(s) 411, 446, 516, 528, 533
SatI GCNGC 5 cut(s) 31, 59, 249, 858, 999
Sau3AI GATC 2 cut(s) 1267, 1525
Sau96I GGNCC 6 cut(s) 149, 422, 801, 1063, 1081, 1376
ScaI AGTACT 1 cut(s) 1400
SchI GAGTC 1 cut(s) 551
ScrFI CCNGG 6 cut(s) 170, 719, 759, 976, 1079, 1163
SduI GDGCHC 1 cut(s) 377
SfaNI GCATC 5 cut(s) 59, 64, 481, 634, 1084
SfcI CTRYAG 1 cut(s) 833
Sfr274I CTCGAG 2 cut(s) 198, 1380
SfuI TTCGAA 2 cut(s) 807, 1028
SinI GGWCC 3 cut(s) 149, 422, 1376
SlaI CTCGAG 2 cut(s) 198, 1380
SmiMI CAYNNNNRTG 1 cut(s) 243
SmlI CTYRAG 5 cut(s) 198, 652, 893, 1380, 1418
SmoI CTYRAG 5 cut(s) 198, 652, 893, 1380, 1418
Sse9I AATT 8 cut(s) 45, 68, 407, 549, 562, 597, 753, 885
SsiI CCGC 4 cut(s) 420, 937, 992, 1477
SspI AATATT 2 cut(s) 178, 330
SspMI CTAG 3 cut(s) 17, 104, 819
StyD4I CCNGG 6 cut(s) 168, 717, 757, 974, 1077, 1161
StyI CCWWGG 1 cut(s) 1407
TaaI ACNGT 6 cut(s) 90, 245, 575, 911, 1353, 1398
TaiI ACGT 3 cut(s) 374, 404, 1051
TaqI TCGA 5 cut(s) 199, 582, 807, 1028, 1381
TasI AATT 8 cut(s) 45, 68, 407, 549, 562, 597, 753, 885
TatI WGTACW 2 cut(s) 1356, 1398
TfiI GAWTC 7 cut(s) 224, 579, 1135, 1142, 1213, 1289, 1326
Tru1I TTAA 5 cut(s) 411, 446, 516, 528, 533
Tru9I TTAA 5 cut(s) 411, 446, 516, 528, 533
TscAI CASTG 2 cut(s) 360, 1270
TseI GCWGC 5 cut(s) 30, 58, 248, 857, 998
TspDTI ATGAA 9 cut(s) 50, 623, 931, 996, 1127, 1130, 1155, 1205, 1214
TspGWI ACGGA 1 cut(s) 554
TspRI CASTG 2 cut(s) 360, 1270
VneI GTGCAC 1 cut(s) 373
VpaK11BI GGWCC 3 cut(s) 149, 422, 1376
XapI RAATTY 3 cut(s) 562, 597, 753
XceI RCATGY 1 cut(s) 1363
XhoI CTCGAG 2 cut(s) 198, 1380
XspI CTAG 3 cut(s) 17, 104, 819
ZrmI AGTACT 1 cut(s) 1400
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.