Rroxscaffold_1G00007390

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0001338
Physical Location & Seq
Forward (+)
9435077 .. 9437329
2253 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00007390.1

Sequence Viewer

Length: 1449 bp
ATGGAGCTTCTTCACTTCGACGCAAAAATGTTAATGTCTCTGGCTGTGCTTGGAGTCATTGGGTTATTAGTACGTCTGTATAATGGGCTTGTGGTGAAGCCGAAGAGGCTGCGGTCCCTGATAACCAAGCAAGGCATCAGCGGTCCCTCGCCAGCTTTTCTCCTGGGAAATATCATGGACATTAAGAAGGCTCGTCCGAGCAGCATTACTAAGGCTCCGACAAGCGAACCCCCCACCTCCCACAACTGTGCTGCTGTTCTCTTCCCCTTTTTTGAGCAGTGGAGGAAGCAATATGGTGAAGTGTTTTTGTTTGCACTCGGCAACACACAGGTACTATATGTGAACAAACCTGAGGCAGTGAAGGAGATTACCACATGCACATCCTGGGACTTGGGGAAGCCTAGTTATCAGTATAAAGAGCGCCGTCCCTTACTTGGTCATGAGTCTACAATTGCTCTGGTAAACTCATGGAACAGTAAGATTGAGGCACAGGGAGGAATTGCAGACATAAAAATGGACCACTATATGAGAAGTTTCTCTGGTGATGTTATCTCAAGGGCATGTTTCGGAAGCAACTATTTGAAAGGCGAAGAGATATTTCAGAAACTAACCGATCTCCAGGAGGCCATGGCCAGGAAACTTTTCTTGACCGGAATACCTGGATTGAGACACCTTCCAACAAAGAGTAATAGGGAAGCTTGGGCATTAGAGAAGGAGGCTGCAACTTTGATACTACGGGTAGTGAAGGAGAGACAGGCTTCGGGGTACGAGAAAGACCTATTGCAAATGATTCTTGAGGGGGCTAAAAACAGTGACCTTAGCCAGGAGGCAACAGACCGTTTCATTGTCGATAACTGCAAAAACATTTACTTGGCTGGATATGAAACCACAGCAGTTTCTGCCACATGGTGCCTCATGTTGTTGGCTTTGAATCAAGAATGGCAAGAACATGTCAGAGCGGAGGTTCTTCAAGTTTGCCAGGGCCAAATTCCAGATGCTGATATGCTACTTAAGATGAAACAGCTAACAATGGTGATTCATGAATCGTTGCGCCTTTATCCCCCAAACACTGTGGTGTCAAGGGAGGCTCTCAAGGACTTGAAATTTGGAGACATTCATGTGCCAAAGGGTGTCAATGTTTGGACAGATGTAGTAACCCTACACACCGATCCTGAAATATGGGGACCGGATTCCTACACATTCAACCCCGAGCGATTTTCCAATGGGATCTCTGGTGCCTGCAAGCTTCCCTACATGTACATGCCTTTTGGGATTGGACCACGAATATGTCTTGGACAGAACTTTGCAATGGTTGAACTCAAGATTCTGATAGCTCTCATACTCTCCAACTTCTCCTTCTCACTATCTCCCAAGTACATCCACAAGCCTTCTCTTAAGTTGGTCATTGAACCAGAACACGGAGTTGATCTCTTGGTAAAAAAGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

482

Amino Acids

54.19

Weight (kDa)

8.7

Isoelectric Point (pI)

42.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 148 - 466 1.1e-66 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 909, 1235
AccBSI CCGCTC 1 cut(s) 959
AccI GTMKAC 1 cut(s) 446
AciI CCGC 3 cut(s) 112, 141, 959
AclWI GGATC 2 cut(s) 1163, 1235
AcoI YGGCCR 1 cut(s) 630
AcsI RAATTY 2 cut(s) 987, 1103
AdeI CACNNNGTG 1 cut(s) 909
AfaI GTAC 5 cut(s) 72, 333, 767, 1259, 1376
AfiI CCNNNNNNNGG 4 cut(s) 434, 633, 823, 1418
AflII CTTAAG 2 cut(s) 1010, 1394
AflIII ACRYGT 2 cut(s) 949, 1254
AgsI TTSAA 7 cut(s) 583, 931, 971, 1102, 1204, 1316, 1409
AjnI CCWGG 7 cut(s) 162, 383, 618, 632, 658, 822, 978
AjuI GAANNNNNNNTTGG 2 cut(s) 1340, 1372
AleI CACNNNNGTG 2 cut(s) 246, 1073
AluBI AGCT 7 cut(s) 7, 155, 698, 1024, 1246, 1334, 1444
AluI AGCT 7 cut(s) 7, 155, 698, 1024, 1246, 1334, 1444
Alw26I GTCTC 4 cut(s) 42, 661, 745, 1104
AlwI GGATC 2 cut(s) 1163, 1235
AlwNI CAGNNNCTG 2 cut(s) 899, 998
Ama87I CYCGRG 1 cut(s) 1208
AoxI GGCC 3 cut(s) 624, 630, 982
ApeKI GCWGC 4 cut(s) 109, 201, 251, 719
ApoI RAATTY 2 cut(s) 987, 1103
ArsI GACNNNNNNTTYG 2 cut(s) 1088, 1120
Asp700I GAANNNNTTC 1 cut(s) 641
AspLEI GCGC 2 cut(s) 423, 1053
AspS9I GGNCC 6 cut(s) 114, 143, 517, 982, 1184, 1277
AsuHPI GGTGA 4 cut(s) 106, 308, 554, 1045
AvaI CYCGRG 1 cut(s) 1208
AvaII GGWCC 5 cut(s) 114, 143, 517, 1184, 1277
AxyI CCTNAGG 1 cut(s) 351
BalI TGGCCA 1 cut(s) 632
BanI GGYRCC 2 cut(s) 909, 1235
BbvI GCAGC 4 cut(s) 96, 213, 238, 706
BceAI ACGGC 1 cut(s) 408
BcgI CGANNNNNNTGC 2 cut(s) 91, 125
BciT130I CCWGG 7 cut(s) 164, 385, 620, 634, 660, 824, 980
BcoDI GTCTC 4 cut(s) 42, 661, 745, 1104
BfaI CTAG 1 cut(s) 402
BfoI RGCGCY 1 cut(s) 424
BfrI CTTAAG 2 cut(s) 1010, 1394
BglI GCCNNNNNGGC 1 cut(s) 106
BisI GCNGC 4 cut(s) 110, 202, 252, 720
BlsI GCNGC 4 cut(s) 111, 203, 253, 721
Bme1390I CCNGG 7 cut(s) 164, 385, 620, 634, 660, 824, 980
Bme18I GGWCC 5 cut(s) 114, 143, 517, 1184, 1277
BmeT110I CYCGRG 1 cut(s) 1208
BmgT120I GGNCC 6 cut(s) 114, 143, 517, 982, 1184, 1277
BmiI GGNNCC 6 cut(s) 116, 145, 216, 911, 1185, 1237
BmrFI CCNGG 7 cut(s) 164, 385, 620, 634, 660, 824, 980
BmsI GCATC 2 cut(s) 144, 985
BplI GAGNNNNNCTC 2 cut(s) 1413, 1445
BpmI CTGGAG 1 cut(s) 602
Bpu10I CCTNAGC 1 cut(s) 818
BpuEI CTTGAG 4 cut(s) 538, 815, 1076, 1304
BsaJI CCNNGG 4 cut(s) 163, 384, 627, 979
BsaWI WCCGGW 2 cut(s) 650, 1186
BsaXI ACNNNNNCTCC 2 cut(s) 199, 229
Bsc4I CCNNNNNNNGG 4 cut(s) 434, 633, 823, 1418
Bse21I CCTNAGG 1 cut(s) 351
Bse3DI GCAATG 1 cut(s) 1314
BseBI CCWGG 7 cut(s) 164, 385, 620, 634, 660, 824, 980
BseDI CCNNGG 4 cut(s) 163, 384, 627, 979
BseGI GGATG 2 cut(s) 380, 1377
BseLI CCNNNNNNNGG 4 cut(s) 434, 633, 823, 1418
BseMI GCAATG 1 cut(s) 1314
BseMII CTCAG 1 cut(s) 342
BseXI GCAGC 4 cut(s) 96, 213, 238, 706
BshFI GGCC 3 cut(s) 626, 632, 984
BshNI GGYRCC 2 cut(s) 909, 1235
BsiHKCI CYCGRG 1 cut(s) 1208
BsiSI CCGG 2 cut(s) 651, 1187
BslFI GGGAC 5 cut(s) 100, 129, 401, 411, 1197
BslI CCNNNNNNNGG 4 cut(s) 434, 633, 823, 1418
BsmAI GTCTC 4 cut(s) 42, 661, 745, 1104
BsmFI GGGAC 5 cut(s) 100, 129, 401, 411, 1197
BsnI GGCC 3 cut(s) 626, 632, 984
BsoBI CYCGRG 1 cut(s) 1208
Bsp1407I TGTACA 1 cut(s) 1257
Bsp143I GATC 4 cut(s) 613, 1168, 1227, 1426
Bsp19I CCATGG 1 cut(s) 627
BspACI CCGC 3 cut(s) 112, 141, 959
BspANI GGCC 3 cut(s) 626, 632, 984
BspCNI CTCAG 1 cut(s) 343
BspHI TCATGA 2 cut(s) 439, 1039
BspLI GGNNCC 6 cut(s) 116, 145, 216, 911, 1185, 1237
BspPI GGATC 2 cut(s) 1163, 1235
BspT107I GGYRCC 2 cut(s) 909, 1235
BspTI CTTAAG 2 cut(s) 1010, 1394
BsrBI CCGCTC 1 cut(s) 959
BsrDI GCAATG 1 cut(s) 1314
BsrGI TGTACA 1 cut(s) 1257
BssECI CCNNGG 4 cut(s) 163, 384, 627, 979
BssMI GATC 4 cut(s) 613, 1168, 1227, 1426
BssT1I CCWWGG 1 cut(s) 627
Bst2UI CCWGG 7 cut(s) 164, 385, 620, 634, 660, 824, 980
Bst4CI ACNGT 5 cut(s) 248, 476, 812, 839, 1072
Bst6I CTCTTC 3 cut(s) 98, 266, 585
BstAFI CTTAAG 2 cut(s) 1010, 1394
BstAPI GCANNNNNTGC 1 cut(s) 899
BstAUI TGTACA 1 cut(s) 1257
BstC8I GCNNGC 3 cut(s) 153, 1240, 1244
BstDEI CTNAG 3 cut(s) 210, 351, 818
BstDSI CCRYGG 1 cut(s) 627
BstENI CCTNNNNNAGG 1 cut(s) 821
BstF5I GGATG 2 cut(s) 380, 1377
BstH2I RGCGCY 1 cut(s) 424
BstHHI GCGC 2 cut(s) 423, 1053
BstKTI GATC 4 cut(s) 616, 1171, 1230, 1429
BstMAI GTCTC 4 cut(s) 42, 661, 745, 1104
BstMBI GATC 4 cut(s) 613, 1168, 1227, 1426
BstMWI GCNNNNNNNGC 2 cut(s) 106, 899
BstNI CCWGG 7 cut(s) 164, 385, 620, 634, 660, 824, 980
BstNSI RCATGY 5 cut(s) 378, 564, 953, 1258, 1264
BstSCI CCNGG 7 cut(s) 162, 383, 618, 632, 658, 822, 978
BstV1I GCAGC 4 cut(s) 96, 213, 238, 706
BstX2I RGATCY 1 cut(s) 1227
BstYI RGATCY 1 cut(s) 1227
Bsu36I CCTNAGG 1 cut(s) 351
BsuRI GGCC 3 cut(s) 626, 632, 984
BtgI CCRYGG 1 cut(s) 627
BtsCI GGATG 2 cut(s) 380, 1377
BtsI GCAGTG 2 cut(s) 284, 363
BtsIMutI CAGTG 4 cut(s) 284, 363, 817, 1068
Cac8I GCNNGC 3 cut(s) 153, 1240, 1244
CaiI CAGNNNCTG 2 cut(s) 899, 998
CciI TCATGA 2 cut(s) 439, 1039
CfoI GCGC 2 cut(s) 423, 1053
Cfr13I GGNCC 6 cut(s) 114, 143, 517, 982, 1184, 1277
CseI GACGC 1 cut(s) 29
Csp6I GTAC 5 cut(s) 71, 332, 766, 1258, 1375
CspCI CAANNNNNGTGG 2 cut(s) 1053, 1088
CviQI GTAC 5 cut(s) 71, 332, 766, 1258, 1375
DdeI CTNAG 3 cut(s) 210, 351, 818
DpnI GATC 4 cut(s) 615, 1170, 1229, 1428
DpnII GATC 4 cut(s) 613, 1168, 1227, 1426
DraIII CACNNNGTG 1 cut(s) 909
EaeI YGGCCR 1 cut(s) 630
Eam1104I CTCTTC 3 cut(s) 98, 266, 585
EarI CTCTTC 3 cut(s) 98, 266, 585
Eco130I CCWWGG 1 cut(s) 627
Eco47I GGWCC 5 cut(s) 114, 143, 517, 1184, 1277
Eco81I CCTNAGG 1 cut(s) 351
Eco88I CYCGRG 1 cut(s) 1208
EcoNI CCTNNNNNAGG 1 cut(s) 821
EcoRII CCWGG 7 cut(s) 162, 383, 618, 632, 658, 822, 978
EcoT14I CCWWGG 1 cut(s) 627
ErhI CCWWGG 1 cut(s) 627
FaqI GGGAC 5 cut(s) 100, 129, 401, 411, 1197
FblI GTMKAC 1 cut(s) 446
Fnu4HI GCNGC 4 cut(s) 110, 202, 252, 720
FokI GGATG 2 cut(s) 367, 1364
Fsp4HI GCNGC 4 cut(s) 110, 202, 252, 720
FspBI CTAG 1 cut(s) 402
GlaI GCGC 2 cut(s) 422, 1052
GluI GCNGC 4 cut(s) 110, 202, 252, 720
GsuI CTGGAG 1 cut(s) 602
HaeII RGCGCY 1 cut(s) 424
HaeIII GGCC 3 cut(s) 626, 632, 984
HapII CCGG 2 cut(s) 651, 1187
HgaI GACGC 1 cut(s) 29
HhaI GCGC 2 cut(s) 423, 1053
Hin6I GCGC 2 cut(s) 421, 1051
HinP1I GCGC 2 cut(s) 421, 1051
HindIII AAGCTT 2 cut(s) 696, 1244
HinfI GANTC 8 cut(s) 54, 443, 790, 931, 1036, 1043, 1190, 1324
HpaII CCGG 2 cut(s) 651, 1187
HphI GGTGA 4 cut(s) 106, 308, 554, 1045
Hpy166II GTNNAC 3 cut(s) 343, 447, 463
Hpy188I TCNGA 6 cut(s) 198, 219, 569, 603, 956, 1329
Hpy188III TCNNGA 8 cut(s) 440, 646, 794, 935, 992, 1040, 1172, 1321
Hpy8I GTNNAC 3 cut(s) 343, 447, 463
Hpy99I CGWCG 1 cut(s) 23
HpyAV CCTTC 7 cut(s) 181, 355, 683, 706, 739, 1366, 1398
HpyCH4III ACNGT 5 cut(s) 248, 476, 812, 839, 1072
HpyCH4IV ACGT 1 cut(s) 73
HpyCH4V TGCA 8 cut(s) 314, 378, 503, 722, 784, 858, 1242, 1307
HpyF10VI GCNNNNNNNGC 2 cut(s) 106, 899
HpyF3I CTNAG 3 cut(s) 210, 351, 818
HpySE526I ACGT 1 cut(s) 73
HspAI GCGC 2 cut(s) 421, 1051
Kzo9I GATC 4 cut(s) 613, 1168, 1227, 1426
LmnI GCTCC 2 cut(s) 4, 220
Lsp1109I GCAGC 4 cut(s) 96, 213, 238, 706
LweI GCATC 2 cut(s) 144, 985
MaeI CTAG 1 cut(s) 402
MaeII ACGT 1 cut(s) 73
MaeIII GTNAC 2 cut(s) 812, 1153
MalI GATC 4 cut(s) 615, 1170, 1229, 1428
MbiI CCGCTC 1 cut(s) 959
MboI GATC 4 cut(s) 613, 1168, 1227, 1426
MboII GAAGA 4 cut(s) 115, 253, 602, 959
MfeI CAATTG 1 cut(s) 450
MflI RGATCY 1 cut(s) 1227
MlsI TGGCCA 1 cut(s) 632
MluCI AATT 4 cut(s) 450, 498, 987, 1103
MluNI TGGCCA 1 cut(s) 632
MlyI GAGTC 2 cut(s) 63, 452
MmeI TCCRAC 3 cut(s) 242, 701, 1371
Mox20I TGGCCA 1 cut(s) 632
MroXI GAANNNNTTC 1 cut(s) 641
MscI TGGCCA 1 cut(s) 632
MseI TTAA 4 cut(s) 32, 183, 1011, 1395
MslI CAYNNNNRTG 6 cut(s) 246, 512, 1073, 1118, 1259, 1285
Msp20I TGGCCA 1 cut(s) 632
MspA1I CMGCKG 1 cut(s) 141
MspCI CTTAAG 2 cut(s) 1010, 1394
MspI CCGG 2 cut(s) 651, 1187
MspR9I CCNGG 7 cut(s) 164, 385, 620, 634, 660, 824, 980
MunI CAATTG 1 cut(s) 450
MvaI CCWGG 7 cut(s) 164, 385, 620, 634, 660, 824, 980
MwoI GCNNNNNNNGC 2 cut(s) 106, 899
NcoI CCATGG 1 cut(s) 627
NdeII GATC 4 cut(s) 613, 1168, 1227, 1426
NlaIV GGNNCC 6 cut(s) 116, 145, 216, 911, 1185, 1237
NmeAIII GCCGAG 1 cut(s) 297
NmuCI GTSAC 1 cut(s) 812
NspI RCATGY 5 cut(s) 378, 564, 953, 1258, 1264
OliI CACNNNNGTG 2 cut(s) 246, 1073
PagI TCATGA 2 cut(s) 439, 1039
PciI ACATGT 2 cut(s) 949, 1254
PdmI GAANNNNTTC 1 cut(s) 641
PfeI GAWTC 6 cut(s) 790, 931, 1036, 1043, 1190, 1324
PfoI TCCNGGA 1 cut(s) 618
PkrI GCNGC 4 cut(s) 111, 203, 253, 721
PleI GAGTC 2 cut(s) 62, 451
PpsI GAGTC 2 cut(s) 62, 451
PscI ACATGT 2 cut(s) 949, 1254
Psp6I CCWGG 7 cut(s) 162, 383, 618, 632, 658, 822, 978
PspGI CCWGG 7 cut(s) 162, 383, 618, 632, 658, 822, 978
PspN4I GGNNCC 6 cut(s) 116, 145, 216, 911, 1185, 1237
PspPI GGNCC 6 cut(s) 114, 143, 517, 982, 1184, 1277
PstNI CAGNNNCTG 2 cut(s) 899, 998
PsuI RGATCY 1 cut(s) 1227
RsaI GTAC 5 cut(s) 72, 333, 767, 1259, 1376
RsaNI GTAC 5 cut(s) 71, 332, 766, 1258, 1375
RseI CAYNNNNRTG 6 cut(s) 246, 512, 1073, 1118, 1259, 1285
SaqAI TTAA 4 cut(s) 32, 183, 1011, 1395
SatI GCNGC 4 cut(s) 110, 202, 252, 720
Sau3AI GATC 4 cut(s) 613, 1168, 1227, 1426
Sau96I GGNCC 6 cut(s) 114, 143, 517, 982, 1184, 1277
SchI GAGTC 2 cut(s) 63, 452
ScrFI CCNGG 7 cut(s) 164, 385, 620, 634, 660, 824, 980
SfaNI GCATC 2 cut(s) 144, 985
SinI GGWCC 5 cut(s) 114, 143, 517, 1184, 1277
SmiMI CAYNNNNRTG 6 cut(s) 246, 512, 1073, 1118, 1259, 1285
SmlI CTYRAG 6 cut(s) 553, 794, 1010, 1091, 1319, 1394
SmoI CTYRAG 6 cut(s) 553, 794, 1010, 1091, 1319, 1394
Sse9I AATT 4 cut(s) 450, 498, 987, 1103
SsiI CCGC 3 cut(s) 112, 141, 959
SspMI CTAG 1 cut(s) 402
StyD4I CCNGG 7 cut(s) 162, 383, 618, 632, 658, 822, 978
StyI CCWWGG 1 cut(s) 627
TaaI ACNGT 5 cut(s) 248, 476, 812, 839, 1072
TaiI ACGT 1 cut(s) 76
TaqI TCGA 2 cut(s) 18, 849
TasI AATT 4 cut(s) 450, 498, 987, 1103
TatI WGTACW 2 cut(s) 1257, 1374
TfiI GAWTC 6 cut(s) 790, 931, 1036, 1043, 1190, 1324
Tru1I TTAA 4 cut(s) 32, 183, 1011, 1395
Tru9I TTAA 4 cut(s) 32, 183, 1011, 1395
TscAI CASTG 4 cut(s) 284, 363, 817, 1075
TseFI GTSAC 1 cut(s) 812
TseI GCWGC 4 cut(s) 109, 201, 251, 719
Tsp45I GTSAC 1 cut(s) 812
TspDTI ATGAA 6 cut(s) 832, 897, 1028, 1031, 1056, 1106
TspGWI ACGGA 1 cut(s) 1434
TspRI CASTG 4 cut(s) 284, 363, 817, 1075
Vha464I CTTAAG 2 cut(s) 1010, 1394
VpaK11BI GGWCC 5 cut(s) 114, 143, 517, 1184, 1277
XagI CCTNNNNNAGG 1 cut(s) 821
XapI RAATTY 2 cut(s) 987, 1103
XceI RCATGY 5 cut(s) 378, 564, 953, 1258, 1264
XmiI GTMKAC 1 cut(s) 446
XmnI GAANNNNTTC 1 cut(s) 641
XspI CTAG 1 cut(s) 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.