Rw5G045050

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001338
Physical Location & Seq
Forward (+)
78928818 .. 78931077
2260 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G045050.1

Sequence Viewer

Length: 1497 bp
ATGGAGCTTCTTCACTTTGATGCAAAAATGTTAATGTCTCTGGCTATGCTTGGAGTCATAGGGTTATTAGTACGTCTGTATAATGGGCTTGTGGTGAAGCCGAAGAGGCTGCGGTCCTTGATAACCAAGCAAGGCATCAGCGGTCCCTCGCCAGCTTTTCTCCTGGGAAATATCATGGACATTAAGAAGGCTCGTCCGAGCAGCATTACTAAGGCTCCGACAAGCGAACCCCCCACCTCCCACAACTGTGCTGCTGTTCTCTTCCCCTTTTTTGAGCAGTGGAGGAAGCAATATGGTGAAGTGTTTGTGTTTGCACTCGGCAACACACAGGTACTATATGTGAACAAACCTGAGGCAGTGAAGGAGATTACCACATGCACATCCTGGGACTTGGGGAAGCCTAGTTATCAGTATAAAGAGCGCCGTCCCTTACTTGGTCATGGTATTCTAACCTCGAACGGTGCCTCATGGGCTCATCAGAGAAAAATCATTGCTCCTGAACTATACATGCAGAAAGTTAAGGGAATGATCAAATTAATCACAGAGTCTACAATTGCTCTGGTAAATTCATGGAACAGTAAGATTGAGGCACAGGGAGGAATTGCAGACATAAAAATGGACCACTATATGAGAAGTTTCTCTGGTGATGTTATCTCAAGGGCGTGTTTCGGAAGCAACTATTTGAAAGGCGAAGAGATATTTCAGAAACTAACCGATCTCCAAGAGGCCATGAGTAATAGGGAAGCTTGGGCATTAGAGAAGGAGGCTGCAACTTTGATACTACGGGTAGTGAAGGAGAGACAGGCTGCGGGGTACGAGAAAGACCTATTGCAAATGATTCTTGAGGGGGCTAAAAACAGTGACCTTAGCCAGGAGGCAACAGACCGTTTCATTGTCGATAACTGCAAAAACATTTACTTGGCTGGATATGAAACCACAGCAGTTTCTGCCACATGGTGCCTCATGTTGTTGGCTTTGAATCAAGAATGGCAAGAACGTGTCAGAGCGGAGGTTCTTCTAGTTTTCAAGGGCCGAATTCCAGATGCTGATATGCTCCTTAAGATGAAACAGCTAACAATGGTGATTCATGAATCGTTGCGCCTTTATCCCCCAAACACTGTGGTGTCAAGGGAGGCTCTCAAGGACTTGAAATTTGGAGACATTCATGTGCCAAAGGGTGTCAATGTATGGACAGATATAGTAACCCTACACACCGATCCTGAAATATGGGGACCGGATTCCTACACATTCAACCCCGAGCGATTTTCCAATGGGATCTCTGGTGCCTGCAAGCTTCCCTACTTGTACATGCCGTTTGGGATTGGACCACGAATATGTCTTGGACAGAACTTTGCAATGGTTGAACTCAAGATTCTGCTAGCTCTCATACTCTCCAACTTCTCCTTCTCACTATCTCCCAAGTACATCCACAAGCCTTCTCTTAAGTTGGTTATTGAACCAGAACACGGAGTTGATCTCTTGGTAAAAAAGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

498

Amino Acids

56.04

Weight (kDa)

8.97

Isoelectric Point (pI)

39.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 90 - 482 1.2e-76 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 461, 957, 1283
AccBSI CCGCTC 1 cut(s) 1007
AccI GTMKAC 1 cut(s) 548
AciI CCGC 4 cut(s) 112, 141, 809, 1007
AclWI GGATC 2 cut(s) 1211, 1283
AcsI RAATTY 3 cut(s) 565, 1035, 1151
AdeI CACNNNGTG 1 cut(s) 957
AfaI GTAC 5 cut(s) 72, 333, 815, 1307, 1424
AfiI CCNNNNNNNGG 3 cut(s) 434, 871, 1466
AflII CTTAAG 2 cut(s) 1058, 1442
AflIII ACRYGT 1 cut(s) 997
AgsI TTSAA 7 cut(s) 685, 979, 1027, 1150, 1252, 1364, 1457
AjnI CCWGG 3 cut(s) 162, 383, 870
AjuI GAANNNNNNNTTGG 2 cut(s) 1388, 1420
AleI CACNNNNGTG 2 cut(s) 246, 1121
AluBI AGCT 7 cut(s) 7, 155, 746, 1072, 1294, 1382, 1492
AluI AGCT 7 cut(s) 7, 155, 746, 1072, 1294, 1382, 1492
Alw26I GTCTC 3 cut(s) 42, 793, 1152
AlwI GGATC 2 cut(s) 1211, 1283
AlwNI CAGNNNCTG 2 cut(s) 947, 1046
Ama87I CYCGRG 1 cut(s) 1256
AoxI GGCC 2 cut(s) 726, 1030
ApeKI GCWGC 5 cut(s) 109, 201, 251, 767, 806
ApoI RAATTY 3 cut(s) 565, 1035, 1151
ArsI GACNNNNNNTTYG 2 cut(s) 1136, 1168
AseI ATTAAT 1 cut(s) 536
AspLEI GCGC 2 cut(s) 423, 1101
AspS9I GGNCC 6 cut(s) 114, 143, 619, 1030, 1232, 1325
AsuHPI GGTGA 4 cut(s) 106, 308, 656, 1093
AsuNHI GCTAGC 1 cut(s) 1378
AvaI CYCGRG 1 cut(s) 1256
AvaII GGWCC 5 cut(s) 114, 143, 619, 1232, 1325
AxyI CCTNAGG 1 cut(s) 351
BanI GGYRCC 3 cut(s) 461, 957, 1283
BanII GRGCYC 1 cut(s) 475
BbvI GCAGC 5 cut(s) 96, 213, 238, 754, 793
BceAI ACGGC 2 cut(s) 408, 1297
BcgI CGANNNNNNTGC 2 cut(s) 91, 125
BciT130I CCWGG 3 cut(s) 164, 385, 872
BclI TGATCA 1 cut(s) 528
BcoDI GTCTC 3 cut(s) 42, 793, 1152
BfaI CTAG 3 cut(s) 402, 1019, 1379
BfoI RGCGCY 1 cut(s) 424
BfrI CTTAAG 2 cut(s) 1058, 1442
BglI GCCNNNNNGGC 2 cut(s) 106, 470
BisI GCNGC 5 cut(s) 110, 202, 252, 768, 807
BlsI GCNGC 5 cut(s) 111, 203, 253, 769, 808
Bme1390I CCNGG 3 cut(s) 164, 385, 872
Bme18I GGWCC 5 cut(s) 114, 143, 619, 1232, 1325
BmeT110I CYCGRG 1 cut(s) 1256
BmgT120I GGNCC 6 cut(s) 114, 143, 619, 1030, 1232, 1325
BmiI GGNNCC 6 cut(s) 145, 216, 463, 959, 1233, 1285
BmrFI CCNGG 3 cut(s) 164, 385, 872
BmsI GCATC 3 cut(s) 10, 144, 1033
BmtI GCTAGC 1 cut(s) 1382
BplI GAGNNNNNCTC 2 cut(s) 1461, 1493
Bpu10I CCTNAGC 1 cut(s) 866
BpuEI CTTGAG 4 cut(s) 640, 863, 1124, 1352
BsaJI CCNNGG 2 cut(s) 163, 384
BsaWI WCCGGW 1 cut(s) 1234
BsaXI ACNNNNNCTCC 2 cut(s) 199, 229
Bsc4I CCNNNNNNNGG 3 cut(s) 434, 871, 1466
Bse21I CCTNAGG 1 cut(s) 351
Bse3DI GCAATG 2 cut(s) 489, 1362
BseBI CCWGG 3 cut(s) 164, 385, 872
BseDI CCNNGG 2 cut(s) 163, 384
BseGI GGATG 2 cut(s) 380, 1425
BseLI CCNNNNNNNGG 3 cut(s) 434, 871, 1466
BseMI GCAATG 2 cut(s) 489, 1362
BseMII CTCAG 1 cut(s) 342
BseXI GCAGC 5 cut(s) 96, 213, 238, 754, 793
BshFI GGCC 2 cut(s) 728, 1032
BshNI GGYRCC 3 cut(s) 461, 957, 1283
BsiHKCI CYCGRG 1 cut(s) 1256
BsiSI CCGG 1 cut(s) 1235
BslFI GGGAC 4 cut(s) 129, 401, 411, 1245
BslI CCNNNNNNNGG 3 cut(s) 434, 871, 1466
BsmAI GTCTC 3 cut(s) 42, 793, 1152
BsmFI GGGAC 4 cut(s) 129, 401, 411, 1245
BsnI GGCC 2 cut(s) 728, 1032
BsoBI CYCGRG 1 cut(s) 1256
Bsp1286I GDGCHC 1 cut(s) 475
Bsp1407I TGTACA 1 cut(s) 1305
Bsp143I GATC 5 cut(s) 528, 715, 1216, 1275, 1474
BspACI CCGC 4 cut(s) 112, 141, 809, 1007
BspANI GGCC 2 cut(s) 728, 1032
BspCNI CTCAG 1 cut(s) 343
BspHI TCATGA 1 cut(s) 1087
BspLI GGNNCC 6 cut(s) 145, 216, 463, 959, 1233, 1285
BspOI GCTAGC 1 cut(s) 1382
BspPI GGATC 2 cut(s) 1211, 1283
BspT107I GGYRCC 3 cut(s) 461, 957, 1283
BspTI CTTAAG 2 cut(s) 1058, 1442
BsrBI CCGCTC 1 cut(s) 1007
BsrDI GCAATG 2 cut(s) 489, 1362
BsrGI TGTACA 1 cut(s) 1305
BssECI CCNNGG 2 cut(s) 163, 384
BssMI GATC 5 cut(s) 528, 715, 1216, 1275, 1474
Bst2UI CCWGG 3 cut(s) 164, 385, 872
Bst4CI ACNGT 6 cut(s) 248, 461, 578, 860, 887, 1120
Bst6I CTCTTC 3 cut(s) 98, 266, 687
BstAFI CTTAAG 2 cut(s) 1058, 1442
BstAPI GCANNNNNTGC 1 cut(s) 947
BstAUI TGTACA 1 cut(s) 1305
BstC8I GCNNGC 4 cut(s) 153, 1288, 1292, 1380
BstDEI CTNAG 3 cut(s) 210, 351, 866
BstENI CCTNNNNNAGG 1 cut(s) 869
BstF5I GGATG 2 cut(s) 380, 1425
BstH2I RGCGCY 1 cut(s) 424
BstHHI GCGC 2 cut(s) 423, 1101
BstKTI GATC 5 cut(s) 531, 718, 1219, 1278, 1477
BstMAI GTCTC 3 cut(s) 42, 793, 1152
BstMBI GATC 5 cut(s) 528, 715, 1216, 1275, 1474
BstMWI GCNNNNNNNGC 3 cut(s) 106, 470, 947
BstNI CCWGG 3 cut(s) 164, 385, 872
BstNSI RCATGY 3 cut(s) 378, 511, 1312
BstSCI CCNGG 3 cut(s) 162, 383, 870
BstV1I GCAGC 5 cut(s) 96, 213, 238, 754, 793
BstX2I RGATCY 1 cut(s) 1275
BstYI RGATCY 1 cut(s) 1275
Bsu36I CCTNAGG 1 cut(s) 351
BsuRI GGCC 2 cut(s) 728, 1032
BtsCI GGATG 2 cut(s) 380, 1425
BtsI GCAGTG 2 cut(s) 284, 363
BtsIMutI CAGTG 4 cut(s) 284, 363, 865, 1116
Cac8I GCNNGC 4 cut(s) 153, 1288, 1292, 1380
CaiI CAGNNNCTG 2 cut(s) 947, 1046
CciI TCATGA 1 cut(s) 1087
CfoI GCGC 2 cut(s) 423, 1101
Cfr13I GGNCC 6 cut(s) 114, 143, 619, 1030, 1232, 1325
Csp6I GTAC 5 cut(s) 71, 332, 814, 1306, 1423
CspCI CAANNNNNGTGG 2 cut(s) 1101, 1136
CviQI GTAC 5 cut(s) 71, 332, 814, 1306, 1423
DdeI CTNAG 3 cut(s) 210, 351, 866
DpnI GATC 5 cut(s) 530, 717, 1218, 1277, 1476
DpnII GATC 5 cut(s) 528, 715, 1216, 1275, 1474
DraIII CACNNNGTG 1 cut(s) 957
Eam1104I CTCTTC 3 cut(s) 98, 266, 687
EarI CTCTTC 3 cut(s) 98, 266, 687
Eco24I GRGCYC 1 cut(s) 475
Eco47I GGWCC 5 cut(s) 114, 143, 619, 1232, 1325
Eco81I CCTNAGG 1 cut(s) 351
Eco88I CYCGRG 1 cut(s) 1256
EcoNI CCTNNNNNAGG 1 cut(s) 869
EcoRI GAATTC 1 cut(s) 1035
EcoRII CCWGG 3 cut(s) 162, 383, 870
EcoT38I GRGCYC 1 cut(s) 475
FaqI GGGAC 4 cut(s) 129, 401, 411, 1245
FauI CCCGC 1 cut(s) 802
FbaI TGATCA 1 cut(s) 528
FblI GTMKAC 1 cut(s) 548
Fnu4HI GCNGC 5 cut(s) 110, 202, 252, 768, 807
FokI GGATG 2 cut(s) 367, 1412
FriOI GRGCYC 1 cut(s) 475
Fsp4HI GCNGC 5 cut(s) 110, 202, 252, 768, 807
FspBI CTAG 3 cut(s) 402, 1019, 1379
GlaI GCGC 2 cut(s) 422, 1100
GluI GCNGC 5 cut(s) 110, 202, 252, 768, 807
HaeII RGCGCY 1 cut(s) 424
HaeIII GGCC 2 cut(s) 728, 1032
HapII CCGG 1 cut(s) 1235
HhaI GCGC 2 cut(s) 423, 1101
Hin6I GCGC 2 cut(s) 421, 1099
HinP1I GCGC 2 cut(s) 421, 1099
HindIII AAGCTT 2 cut(s) 744, 1292
HinfI GANTC 8 cut(s) 54, 545, 838, 979, 1084, 1091, 1238, 1372
HpaII CCGG 1 cut(s) 1235
HphI GGTGA 4 cut(s) 106, 308, 656, 1093
Hpy166II GTNNAC 2 cut(s) 343, 549
Hpy188I TCNGA 6 cut(s) 198, 219, 480, 671, 705, 1004
Hpy188III TCNNGA 7 cut(s) 497, 842, 983, 1040, 1088, 1220, 1369
Hpy8I GTNNAC 2 cut(s) 343, 549
HpyAV CCTTC 6 cut(s) 181, 355, 754, 787, 1414, 1446
HpyCH4III ACNGT 6 cut(s) 248, 461, 578, 860, 887, 1120
HpyCH4IV ACGT 2 cut(s) 73, 997
HpyF10VI GCNNNNNNNGC 3 cut(s) 106, 470, 947
HpyF3I CTNAG 3 cut(s) 210, 351, 866
HpySE526I ACGT 2 cut(s) 73, 997
HspAI GCGC 2 cut(s) 421, 1099
Ksp22I TGATCA 1 cut(s) 528
Kzo9I GATC 5 cut(s) 528, 715, 1216, 1275, 1474
LmnI GCTCC 4 cut(s) 4, 220, 499, 1059
Lsp1109I GCAGC 5 cut(s) 96, 213, 238, 754, 793
LweI GCATC 3 cut(s) 10, 144, 1033
MaeI CTAG 3 cut(s) 402, 1019, 1379
MaeII ACGT 2 cut(s) 73, 997
MaeIII GTNAC 2 cut(s) 860, 1201
MalI GATC 5 cut(s) 530, 717, 1218, 1277, 1476
MbiI CCGCTC 1 cut(s) 1007
MboI GATC 5 cut(s) 528, 715, 1216, 1275, 1474
MboII GAAGA 4 cut(s) 115, 253, 704, 1007
MfeI CAATTG 1 cut(s) 552
MflI RGATCY 1 cut(s) 1275
MhlI GDGCHC 1 cut(s) 475
MluCI AATT 6 cut(s) 533, 552, 565, 600, 1035, 1151
MlyI GAGTC 2 cut(s) 63, 554
MmeI TCCRAC 2 cut(s) 242, 1419
MseI TTAA 6 cut(s) 32, 183, 519, 536, 1059, 1443
MslI CAYNNNNRTG 6 cut(s) 18, 246, 614, 1121, 1166, 1333
MspA1I CMGCKG 1 cut(s) 141
MspCI CTTAAG 2 cut(s) 1058, 1442
MspI CCGG 1 cut(s) 1235
MspR9I CCNGG 3 cut(s) 164, 385, 872
MunI CAATTG 1 cut(s) 552
MvaI CCWGG 3 cut(s) 164, 385, 872
MwoI GCNNNNNNNGC 3 cut(s) 106, 470, 947
NdeII GATC 5 cut(s) 528, 715, 1216, 1275, 1474
NheI GCTAGC 1 cut(s) 1378
NlaIV GGNNCC 6 cut(s) 145, 216, 463, 959, 1233, 1285
NmeAIII GCCGAG 1 cut(s) 297
NmuCI GTSAC 1 cut(s) 860
NspI RCATGY 3 cut(s) 378, 511, 1312
OliI CACNNNNGTG 2 cut(s) 246, 1121
PagI TCATGA 1 cut(s) 1087
PfeI GAWTC 6 cut(s) 838, 979, 1084, 1091, 1238, 1372
PkrI GCNGC 5 cut(s) 111, 203, 253, 769, 808
PleI GAGTC 2 cut(s) 62, 553
PpsI GAGTC 2 cut(s) 62, 553
PshBI ATTAAT 1 cut(s) 536
Psp6I CCWGG 3 cut(s) 162, 383, 870
PspGI CCWGG 3 cut(s) 162, 383, 870
PspN4I GGNNCC 6 cut(s) 145, 216, 463, 959, 1233, 1285
PspPI GGNCC 6 cut(s) 114, 143, 619, 1030, 1232, 1325
PstNI CAGNNNCTG 2 cut(s) 947, 1046
PsuI RGATCY 1 cut(s) 1275
RsaI GTAC 5 cut(s) 72, 333, 815, 1307, 1424
RsaNI GTAC 5 cut(s) 71, 332, 814, 1306, 1423
RseI CAYNNNNRTG 6 cut(s) 18, 246, 614, 1121, 1166, 1333
SaqAI TTAA 6 cut(s) 32, 183, 519, 536, 1059, 1443
SatI GCNGC 5 cut(s) 110, 202, 252, 768, 807
Sau3AI GATC 5 cut(s) 528, 715, 1216, 1275, 1474
Sau96I GGNCC 6 cut(s) 114, 143, 619, 1030, 1232, 1325
SchI GAGTC 2 cut(s) 63, 554
ScrFI CCNGG 3 cut(s) 164, 385, 872
SduI GDGCHC 1 cut(s) 475
SfaNI GCATC 3 cut(s) 10, 144, 1033
SinI GGWCC 5 cut(s) 114, 143, 619, 1232, 1325
SmiMI CAYNNNNRTG 6 cut(s) 18, 246, 614, 1121, 1166, 1333
SmlI CTYRAG 6 cut(s) 655, 842, 1058, 1139, 1367, 1442
SmoI CTYRAG 6 cut(s) 655, 842, 1058, 1139, 1367, 1442
Sse9I AATT 6 cut(s) 533, 552, 565, 600, 1035, 1151
SsiI CCGC 4 cut(s) 112, 141, 809, 1007
SspMI CTAG 3 cut(s) 402, 1019, 1379
StyD4I CCNGG 3 cut(s) 162, 383, 870
TaaI ACNGT 6 cut(s) 248, 461, 578, 860, 887, 1120
TaiI ACGT 2 cut(s) 76, 1000
TaqI TCGA 2 cut(s) 455, 897
TasI AATT 6 cut(s) 533, 552, 565, 600, 1035, 1151
TatI WGTACW 2 cut(s) 1305, 1422
TfiI GAWTC 6 cut(s) 838, 979, 1084, 1091, 1238, 1372
Tru1I TTAA 6 cut(s) 32, 183, 519, 536, 1059, 1443
Tru9I TTAA 6 cut(s) 32, 183, 519, 536, 1059, 1443
TscAI CASTG 4 cut(s) 284, 363, 865, 1123
TseFI GTSAC 1 cut(s) 860
TseI GCWGC 5 cut(s) 109, 201, 251, 767, 806
Tsp45I GTSAC 1 cut(s) 860
TspDTI ATGAA 7 cut(s) 558, 880, 945, 1076, 1079, 1104, 1154
TspGWI ACGGA 1 cut(s) 1482
TspRI CASTG 4 cut(s) 284, 363, 865, 1123
Vha464I CTTAAG 2 cut(s) 1058, 1442
VpaK11BI GGWCC 5 cut(s) 114, 143, 619, 1232, 1325
VspI ATTAAT 1 cut(s) 536
XagI CCTNNNNNAGG 1 cut(s) 869
XapI RAATTY 3 cut(s) 565, 1035, 1151
XceI RCATGY 3 cut(s) 378, 511, 1312
XmiI GTMKAC 1 cut(s) 548
XspI CTAG 3 cut(s) 402, 1019, 1379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.