Rh5BG505200

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001338
Physical Location & Seq
Reverse (-)
80252789 .. 80255987
3199 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG505200.1

Sequence Viewer

Length: 1155 bp
ATGGAGCTCCTCCACGGGTTCGATGTGAAAATGTTGGTGTCGACGGTGGCGCTGATCGGGTTCGTGGGGTTGCTGGTGCGGTTGTATAAGGGGCTAGTGGCGGAGCCGAAGAGGCTGAGATCCCTACTGGCCAAGCAAGGCATCACCGGACCTCCACAAGCTTTGCTTCTCGGGAATATCTCGGAGATAAAGAGGGCTCACGGCCGCGGTGGCAATGCTAAGGCTCCGAGCAGCGAACCACCCACCTCACACAACCTGGCTGCTACTATCTTCCCCTTCTTTGATAAGTGGCGGAAGCAATTTGGTGACGTGTTTGTGTTTGGTCTTGGCACCACGCAAATCCTATATGTGATCAAACCAGAAGCAGTGAGGGAGATCACAACCTGCACATCCTTGGACTTGGGGAAACCCACATACCAATTCAAACAGCGTGGTCCTTTACTTGGTAAAGGTATTTTGACCTCCAATGGAGCCTCGTGGGCTCATCAGCGCAAGGTCATGGCTCCTGAACTTTACATGGAGAAGGTCAAGGGAATGATCAACTTAATTACCGAATCCTCGAATACTCTGGTGAACTTGTGGAACGGTAAAATTGATGCTGGCAAAGGAGTTGCAGACATAAAAATCGACAACGATTTGAGAAGCTTCTCTGGTGATGTTATCTCGAGGGCATGTTTTGGGAGCAGTTATACCAAAGGGGTACAGATTTTTGATAAACTAAGGCAACTCCAGGAGGCCATGGGCAGGAAAGTCTTTGGGGGTGCAGTTCCTGGAATGAGACACCTTCCTACAAAGAGCAATAGGGAAGAATGGGCATTAGAAAAGGAGGTTGCGAGGTTGATACTGCAATTAGTGAAGGAGAGACAGGCAGCTGGGCACGAAAAAGACTTGTTGCAAATGATTCTTGAGGGTGCAAGAGCCAGTGATCTCAGTCAAGATGCAGCAGACAGCTTCATTGTTGATAATTGCAAGAACATATACTTAGCTGGATATGAAACCACAGCTACTTCTGCCACATGGTGCCTCATGCTGTTGGCTTCGAATCAAGAATGGCGAGAACGAATTAGAGCAGAGGTCCTTCAAGTTTGCCAAGGCCGCGTCCCAGATGCTGATATGCTTCGCAAGATGAAACAGGTATCACTAGTTGATCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

384

Amino Acids

42.5

Weight (kDa)

9.42

Isoelectric Point (pI)

36.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 90 - 377 1.2e-33 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 392
AccB1I GGYRCC 2 cut(s) 329, 1020
AccI GTMKAC 1 cut(s) 41
AccII CGCG 2 cut(s) 207, 1098
AciI CCGC 6 cut(s) 79, 101, 205, 207, 292, 1096
AclWI GGATC 1 cut(s) 114
AcoI YGGCCR 2 cut(s) 129, 202
AdeI CACNNNGTG 1 cut(s) 1020
AfaI GTAC 1 cut(s) 702
AfiI CCNNNNNNNGG 2 cut(s) 443, 744
AflIII ACRYGT 1 cut(s) 309
AgsI TTSAA 2 cut(s) 424, 1082
AhlI ACTAGT 1 cut(s) 1141
AjiI CACGTC 1 cut(s) 310
AjnI CCWGG 3 cut(s) 255, 729, 769
AluBI AGCT 7 cut(s) 7, 161, 645, 872, 951, 986, 1004
AluI AGCT 7 cut(s) 7, 161, 645, 872, 951, 986, 1004
Alw21I GWGCWC 1 cut(s) 9
Alw26I GTCTC 2 cut(s) 772, 856
AlwI GGATC 1 cut(s) 114
AlwNI CAGNNNCTG 2 cut(s) 770, 1109
Ama87I CYCGRG 2 cut(s) 170, 664
AoxI GGCC 4 cut(s) 129, 202, 735, 1093
ApeKI GCWGC 4 cut(s) 231, 260, 869, 941
AspLEI GCGC 2 cut(s) 52, 492
AspS9I GGNCC 3 cut(s) 149, 434, 1075
AsuHPI GGTGA 4 cut(s) 136, 317, 583, 665
AsuII TTCGAA 1 cut(s) 1040
AvaI CYCGRG 2 cut(s) 170, 664
AvaII GGWCC 3 cut(s) 149, 434, 1075
BaeGI GKGCMC 1 cut(s) 879
BalI TGGCCA 1 cut(s) 131
BanI GGYRCC 2 cut(s) 329, 1020
BanII GRGCYC 3 cut(s) 9, 199, 484
BauI CACGAG 1 cut(s) 475
Bbv12I GWGCWC 1 cut(s) 9
BbvI GCAGC 4 cut(s) 243, 247, 881, 953
BceAI ACGGC 1 cut(s) 217
BciT130I CCWGG 3 cut(s) 257, 731, 771
BclI TGATCA 3 cut(s) 351, 537, 1147
BcoDI GTCTC 2 cut(s) 772, 856
BcuI ACTAGT 1 cut(s) 1141
BfaI CTAG 3 cut(s) 95, 1142, 1153
BfoI RGCGCY 1 cut(s) 53
BfuAI ACCTGC 1 cut(s) 392
BglI GCCNNNNNGGC 3 cut(s) 112, 210, 479
BisI GCNGC 6 cut(s) 205, 232, 261, 870, 942, 1096
BlsI GCNGC 6 cut(s) 206, 233, 262, 871, 943, 1097
Bme1390I CCNGG 3 cut(s) 257, 731, 771
Bme18I GGWCC 3 cut(s) 149, 434, 1075
BmeT110I CYCGRG 2 cut(s) 170, 664
BmgBI CACGTC 1 cut(s) 310
BmgT120I GGNCC 3 cut(s) 149, 434, 1075
BmiI GGNNCC 6 cut(s) 105, 225, 331, 472, 504, 1022
BmrFI CCNGG 3 cut(s) 257, 731, 771
BmsI GCATC 4 cut(s) 150, 586, 928, 1096
BpmI CTGGAG 1 cut(s) 713
Bpu10I CCTNAGC 1 cut(s) 219
Bpu14I TTCGAA 1 cut(s) 1040
BpuEI CTTGAG 1 cut(s) 926
BsaJI CCNNGG 5 cut(s) 13, 205, 393, 738, 1090
BsaWI WCCGGW 1 cut(s) 146
BsaXI ACNNNNNCTCC 2 cut(s) 136, 166
Bsc4I CCNNNNNNNGG 2 cut(s) 443, 744
Bse1I ACTGG 2 cut(s) 132, 921
Bse3DI GCAATG 1 cut(s) 220
BseBI CCWGG 3 cut(s) 257, 731, 771
BseDI CCNNGG 5 cut(s) 13, 205, 393, 738, 1090
BseGI GGATG 1 cut(s) 389
BseLI CCNNNNNNNGG 2 cut(s) 443, 744
BseMI GCAATG 1 cut(s) 220
BseMII CTCAG 2 cut(s) 107, 943
BseNI ACTGG 2 cut(s) 132, 921
BseSI GKGCMC 1 cut(s) 879
BseX3I CGGCCG 1 cut(s) 202
BseXI GCAGC 4 cut(s) 243, 247, 881, 953
BseYI CCCAGC 1 cut(s) 872
BsgI GTGCAG 2 cut(s) 370, 783
Bsh1236I CGCG 2 cut(s) 207, 1098
Bsh1285I CGRYCG 1 cut(s) 205
BshFI GGCC 4 cut(s) 131, 204, 737, 1095
BshNI GGYRCC 2 cut(s) 329, 1020
BsiEI CGRYCG 1 cut(s) 205
BsiHKAI GWGCWC 1 cut(s) 9
BsiHKCI CYCGRG 2 cut(s) 170, 664
BsiSI CCGG 1 cut(s) 147
BslFI GGGAC 1 cut(s) 1085
BslI CCNNNNNNNGG 2 cut(s) 443, 744
BsmAI GTCTC 2 cut(s) 772, 856
BsmFI GGGAC 1 cut(s) 1085
BsnI GGCC 4 cut(s) 131, 204, 737, 1095
BsoBI CYCGRG 2 cut(s) 170, 664
Bsp119I TTCGAA 1 cut(s) 1040
Bsp1286I GDGCHC 4 cut(s) 9, 199, 484, 879
Bsp143I GATC 7 cut(s) 54, 119, 351, 375, 537, 925, 1147
Bsp19I CCATGG 1 cut(s) 738
BspACI CCGC 6 cut(s) 79, 101, 205, 207, 292, 1096
BspANI GGCC 4 cut(s) 131, 204, 737, 1095
BspCNI CTCAG 2 cut(s) 108, 942
BspFNI CGCG 2 cut(s) 207, 1098
BspLI GGNNCC 6 cut(s) 105, 225, 331, 472, 504, 1022
BspMI ACCTGC 1 cut(s) 392
BspPI GGATC 1 cut(s) 114
BspT104I TTCGAA 1 cut(s) 1040
BspT107I GGYRCC 2 cut(s) 329, 1020
BsrDI GCAATG 1 cut(s) 220
BsrI ACTGG 2 cut(s) 132, 921
BssECI CCNNGG 5 cut(s) 13, 205, 393, 738, 1090
BssMI GATC 7 cut(s) 54, 119, 351, 375, 537, 925, 1147
BssSI CACGAG 1 cut(s) 475
BssT1I CCWWGG 3 cut(s) 393, 738, 1090
Bst2BI CACGAG 1 cut(s) 475
Bst2UI CCWGG 3 cut(s) 257, 731, 771
Bst4CI ACNGT 2 cut(s) 46, 587
Bst6I CTCTTC 1 cut(s) 104
BstBI TTCGAA 1 cut(s) 1040
BstC8I GCNNGC 1 cut(s) 601
BstDEI CTNAG 5 cut(s) 116, 219, 719, 929, 982
BstDSI CCRYGG 3 cut(s) 13, 205, 738
BstF5I GGATG 1 cut(s) 389
BstFNI CGCG 2 cut(s) 207, 1098
BstH2I RGCGCY 1 cut(s) 53
BstHHI GCGC 2 cut(s) 52, 492
BstKTI GATC 7 cut(s) 57, 122, 354, 378, 540, 928, 1150
BstMAI GTCTC 2 cut(s) 772, 856
BstMBI GATC 7 cut(s) 54, 119, 351, 375, 537, 925, 1147
BstMCI CGRYCG 1 cut(s) 205
BstMWI GCNNNNNNNGC 5 cut(s) 112, 210, 479, 1010, 1095
BstNI CCWGG 3 cut(s) 257, 731, 771
BstNSI RCATGY 1 cut(s) 675
BstSCI CCNGG 3 cut(s) 255, 729, 769
BstSLI GKGCMC 1 cut(s) 879
BstUI CGCG 2 cut(s) 207, 1098
BstV1I GCAGC 4 cut(s) 243, 247, 881, 953
BstX2I RGATCY 1 cut(s) 119
BstYI RGATCY 1 cut(s) 119
BstZI CGGCCG 1 cut(s) 202
BsuRI GGCC 4 cut(s) 131, 204, 737, 1095
BtgI CCRYGG 3 cut(s) 13, 205, 738
BtrI CACGTC 1 cut(s) 310
BtsCI GGATG 1 cut(s) 389
BtsI GCAGTG 1 cut(s) 372
BtsIMutI CAGTG 2 cut(s) 372, 928
BveI ACCTGC 1 cut(s) 392
Cac8I GCNNGC 1 cut(s) 601
CaiI CAGNNNCTG 2 cut(s) 770, 1109
CfoI GCGC 2 cut(s) 52, 492
Cfr13I GGNCC 3 cut(s) 149, 434, 1075
Cfr42I CCGCGG 1 cut(s) 208
CseI GACGC 1 cut(s) 1087
Csp6I GTAC 1 cut(s) 701
CspCI CAANNNNNGTGG 4 cut(s) 144, 179, 412, 447
CviAII CATG 6 cut(s) 499, 517, 672, 739, 1017, 1027
CviQI GTAC 1 cut(s) 701
DdeI CTNAG 5 cut(s) 116, 219, 719, 929, 982
DpnI GATC 7 cut(s) 56, 121, 353, 377, 539, 927, 1149
DpnII GATC 7 cut(s) 54, 119, 351, 375, 537, 925, 1147
DraIII CACNNNGTG 1 cut(s) 1020
EaeI YGGCCR 2 cut(s) 129, 202
EagI CGGCCG 1 cut(s) 202
Eam1104I CTCTTC 1 cut(s) 104
EarI CTCTTC 1 cut(s) 104
EciI GGCGGA 2 cut(s) 116, 307
Ecl136II GAGCTC 1 cut(s) 7
EclXI CGGCCG 1 cut(s) 202
Eco130I CCWWGG 3 cut(s) 393, 738, 1090
Eco24I GRGCYC 3 cut(s) 9, 199, 484
Eco47I GGWCC 3 cut(s) 149, 434, 1075
Eco52I CGGCCG 1 cut(s) 202
Eco53kI GAGCTC 1 cut(s) 7
Eco88I CYCGRG 2 cut(s) 170, 664
EcoICRI GAGCTC 1 cut(s) 7
EcoO109I RGGNCCY 1 cut(s) 1075
EcoRII CCWGG 3 cut(s) 255, 729, 769
EcoT14I CCWWGG 3 cut(s) 393, 738, 1090
EcoT38I GRGCYC 3 cut(s) 9, 199, 484
ErhI CCWWGG 3 cut(s) 393, 738, 1090
FaeI CATG 6 cut(s) 502, 520, 675, 742, 1020, 1030
FalI AAGNNNNNCTT 2 cut(s) 150, 182
FaqI GGGAC 1 cut(s) 1085
FatI CATG 6 cut(s) 498, 516, 671, 738, 1016, 1026
FbaI TGATCA 3 cut(s) 351, 537, 1147
FblI GTMKAC 1 cut(s) 41
Fnu4HI GCNGC 6 cut(s) 205, 232, 261, 870, 942, 1096
FokI GGATG 1 cut(s) 376
FriOI GRGCYC 3 cut(s) 9, 199, 484
Fsp4HI GCNGC 6 cut(s) 205, 232, 261, 870, 942, 1096
FspBI CTAG 3 cut(s) 95, 1142, 1153
GlaI GCGC 2 cut(s) 51, 491
GluI GCNGC 6 cut(s) 205, 232, 261, 870, 942, 1096
GsaI CCCAGC 1 cut(s) 876
GsuI CTGGAG 1 cut(s) 713
HaeII RGCGCY 1 cut(s) 53
HaeIII GGCC 4 cut(s) 131, 204, 737, 1095
HapII CCGG 1 cut(s) 147
HgaI GACGC 1 cut(s) 1087
HhaI GCGC 2 cut(s) 52, 492
Hin1II CATG 6 cut(s) 502, 520, 675, 742, 1020, 1030
Hin6I GCGC 2 cut(s) 50, 490
HinP1I GCGC 2 cut(s) 50, 490
HincII GTYRAC 1 cut(s) 42
HindII GTYRAC 1 cut(s) 42
HindIII AAGCTT 2 cut(s) 159, 643
HinfI GANTC 3 cut(s) 554, 901, 1042
HpaII CCGG 1 cut(s) 147
HphI GGTGA 4 cut(s) 136, 317, 583, 665
Hpy166II GTNNAC 2 cut(s) 42, 574
Hpy188I TCNGA 2 cut(s) 184, 228
Hpy188III TCNNGA 6 cut(s) 172, 506, 664, 905, 935, 1046
Hpy8I GTNNAC 2 cut(s) 42, 574
Hpy99I CGWCG 1 cut(s) 46
HpyAV CCTTC 5 cut(s) 286, 517, 794, 850, 1088
HpyCH4III ACNGT 2 cut(s) 46, 587
HpyCH4IV ACGT 1 cut(s) 309
HpyCH4V TGCA 8 cut(s) 387, 614, 764, 847, 895, 914, 941, 969
HpyF10VI GCNNNNNNNGC 5 cut(s) 112, 210, 479, 1010, 1095
HpyF3I CTNAG 5 cut(s) 116, 219, 719, 929, 982
HpySE526I ACGT 1 cut(s) 309
Hsp92II CATG 6 cut(s) 502, 520, 675, 742, 1020, 1030
HspAI GCGC 2 cut(s) 50, 490
Ksp22I TGATCA 3 cut(s) 351, 537, 1147
KspI CCGCGG 1 cut(s) 208
Kzo9I GATC 7 cut(s) 54, 119, 351, 375, 537, 925, 1147
LmnI GCTCC 7 cut(s) 4, 12, 103, 229, 470, 508, 681
Lsp1109I GCAGC 4 cut(s) 243, 247, 881, 953
LweI GCATC 4 cut(s) 150, 586, 928, 1096
MaeI CTAG 3 cut(s) 95, 1142, 1153
MaeII ACGT 1 cut(s) 309
MaeIII GTNAC 1 cut(s) 305
MalI GATC 7 cut(s) 56, 121, 353, 377, 539, 927, 1149
MboI GATC 7 cut(s) 54, 119, 351, 375, 537, 925, 1147
MboII GAAGA 3 cut(s) 121, 262, 818
MflI RGATCY 1 cut(s) 119
MhlI GDGCHC 4 cut(s) 9, 199, 484, 879
MlsI TGGCCA 1 cut(s) 131
MluCI AATT 7 cut(s) 299, 419, 546, 591, 848, 964, 1062
MluNI TGGCCA 1 cut(s) 131
Mox20I TGGCCA 1 cut(s) 131
MscI TGGCCA 1 cut(s) 131
MseI TTAA 1 cut(s) 545
Msp20I TGGCCA 1 cut(s) 131
MspA1I CMGCKG 2 cut(s) 207, 872
MspI CCGG 1 cut(s) 147
MspR9I CCNGG 3 cut(s) 257, 731, 771
MvaI CCWGG 3 cut(s) 257, 731, 771
MvnI CGCG 2 cut(s) 207, 1098
MwoI GCNNNNNNNGC 5 cut(s) 112, 210, 479, 1010, 1095
NcoI CCATGG 1 cut(s) 738
NdeII GATC 7 cut(s) 54, 119, 351, 375, 537, 925, 1147
NlaIII CATG 6 cut(s) 502, 520, 675, 742, 1020, 1030
NlaIV GGNNCC 6 cut(s) 105, 225, 331, 472, 504, 1022
NmuCI GTSAC 1 cut(s) 305
NspI RCATGY 1 cut(s) 675
NspV TTCGAA 1 cut(s) 1040
PaeR7I CTCGAG 1 cut(s) 664
PfeI GAWTC 3 cut(s) 554, 901, 1042
PfoI TCCNGGA 2 cut(s) 729, 769
PkrI GCNGC 6 cut(s) 206, 233, 262, 871, 943, 1097
PpuMI RGGWCCY 1 cut(s) 1075
Psp124BI GAGCTC 1 cut(s) 9
Psp5II RGGWCCY 1 cut(s) 1075
Psp6I CCWGG 3 cut(s) 255, 729, 769
PspFI CCCAGC 1 cut(s) 872
PspGI CCWGG 3 cut(s) 255, 729, 769
PspN4I GGNNCC 6 cut(s) 105, 225, 331, 472, 504, 1022
PspPI GGNCC 3 cut(s) 149, 434, 1075
PspPPI RGGWCCY 1 cut(s) 1075
PstNI CAGNNNCTG 2 cut(s) 770, 1109
PsuI RGATCY 1 cut(s) 119
PvuII CAGCTG 1 cut(s) 872
RsaI GTAC 1 cut(s) 702
RsaNI GTAC 1 cut(s) 701
SacI GAGCTC 1 cut(s) 9
SacII CCGCGG 1 cut(s) 208
SalI GTCGAC 1 cut(s) 40
SaqAI TTAA 1 cut(s) 545
SatI GCNGC 6 cut(s) 205, 232, 261, 870, 942, 1096
Sau3AI GATC 7 cut(s) 54, 119, 351, 375, 537, 925, 1147
Sau96I GGNCC 3 cut(s) 149, 434, 1075
ScrFI CCNGG 3 cut(s) 257, 731, 771
SduI GDGCHC 4 cut(s) 9, 199, 484, 879
SfaNI GCATC 4 cut(s) 150, 586, 928, 1096
Sfr274I CTCGAG 1 cut(s) 664
Sfr303I CCGCGG 1 cut(s) 208
SfuI TTCGAA 1 cut(s) 1040
SgrBI CCGCGG 1 cut(s) 208
SinI GGWCC 3 cut(s) 149, 434, 1075
SlaI CTCGAG 1 cut(s) 664
SmlI CTYRAG 2 cut(s) 664, 905
SmoI CTYRAG 2 cut(s) 664, 905
SpeI ACTAGT 1 cut(s) 1141
Sse9I AATT 7 cut(s) 299, 419, 546, 591, 848, 964, 1062
SsiI CCGC 6 cut(s) 79, 101, 205, 207, 292, 1096
SspMI CTAG 3 cut(s) 95, 1142, 1153
SstI GAGCTC 1 cut(s) 9
StyD4I CCNGG 3 cut(s) 255, 729, 769
StyI CCWWGG 3 cut(s) 393, 738, 1090
TaaI ACNGT 2 cut(s) 46, 587
TaiI ACGT 1 cut(s) 312
TaqI TCGA 6 cut(s) 21, 41, 560, 627, 665, 1040
TasI AATT 7 cut(s) 299, 419, 546, 591, 848, 964, 1062
TauI GCSGC 2 cut(s) 207, 1098
TfiI GAWTC 3 cut(s) 554, 901, 1042
Tru1I TTAA 1 cut(s) 545
Tru9I TTAA 1 cut(s) 545
TscAI CASTG 2 cut(s) 372, 928
TseFI GTSAC 1 cut(s) 305
TseI GCWGC 4 cut(s) 231, 260, 869, 941
Tsp45I GTSAC 1 cut(s) 305
TspDTI ATGAA 3 cut(s) 943, 1008, 1142
TspRI CASTG 2 cut(s) 372, 928
VpaK11BI GGWCC 3 cut(s) 149, 434, 1075
XceI RCATGY 1 cut(s) 675
XhoI CTCGAG 1 cut(s) 664
XmiI GTMKAC 1 cut(s) 41
XspI CTAG 3 cut(s) 95, 1142, 1153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.