FvH4_3g46010

Dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0002881
Physical Location & Seq
Reverse (-)
38203476 .. 38205447
1972 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g46010.t1

Sequence Viewer

Length: 1092 bp
ATGACGTCTGAAGCAACTGTATCATCTGCAGAGGAAGTGCAGGCATATGGATGGGCTGCTAGAGATTCCTCAGGAATTCTCTCTCCCTTCTACTTCACCAGGAGGACTATTGGTATCAATGATATCAGCATGAAGATACTCTACTGTGGTATTTGCCACTCAGATCTTCACCTTGCTAAGGACGAAGTCGGAATGACCATCTATCCCATCGTCCCAGGACATGAGATTGTCGGAGAAGTGACCGGAGTTGGGAGCAACGTGACGAAATTCAAAGTAGGAGACATAGCCGGAGTTGGGTGCATGGTGGGTTCGTGTGGTTCGTGTACTAACTGCACACGAGACTTGGAAAATTACTGCCCCAAAATGATATGGACCTATAACAAACACTACCCCGATGGATCCAGAACGTTTGGGGGATACTCCGACAAGCTAGTAGTCGAGGAGCATTTCGTAGTCCGAATTCCAGATAACCTGTCACTGGAAGGTGTTGCACCTTTGCTATGTGCTGGGATTACGGTGTACAGCCCTATGAAACACTTCGGACTTTTTGAGCATGGTAAGCACTTGGGTGTGGTAGGGCTTGGTGGCTTAGGTCATTTGGCTGTTAAGTTTGCCAAGGCCGTGGGAATGAAAGTCACTGTGATAAGTACCTCCCCTAATAAGAAGAAGGAAGCAATGGAACAACTCGGTGTTGACGATTTTCTTATCAGTCGCGACCAAGAGCAATTTCAGGCTGCTATGGATACAATGGATGGTATCATCGACACAGTTTCAGCCCCTCATTCTCTATTGCCATTGATGGACTTGTTGAAGACGGATGGAAAGCTAATTGTGGTGGGTGCTCCTCTGCAACCTCCTGAACTACCAGTGTTTCCTTTAATCATGGGGAGGAAGTTGATTGCTGGTAGTGCAATTGGGGGAATGAAAGAGACTCAAGAGATGATAGACTTTGCAGCAAAGCACAACATCACGGCCGATGTCGAAGTCATTCCAATGGACTATGTGAACACTGCTCTTGAACGAGTTGGCAAGGTTGATGTGAAGTACCGCTTTGTTATTGATGTTGCAAACACCCTAAATTCTCCATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
ADH_N ADH_zinc_N

Protein Analysis

364

Amino Acids

39.31

Weight (kDa)

5.66

Isoelectric Point (pI)

29.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 40 - 156 6.7e-23 Alcohol dehydrogenase GroES-like domain
2-Hacid_dh_C PF02826 185 - 229 2.7e-06 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
ADH_zinc_N PF00107 196 - 319 4.3e-22 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 8
AccII CGCG 1 cut(s) 714
AciI CCGC 1 cut(s) 1048
AclI AACGTT 1 cut(s) 407
AclWI GGATC 2 cut(s) 393, 406
AcoI YGGCCR 1 cut(s) 972
AcsI RAATTY 4 cut(s) 75, 266, 459, 1078
AcuI CTGAAG 1 cut(s) 30
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 4 cut(s) 325, 521, 649, 1046
AfiI CCNNNNNNNGG 4 cut(s) 178, 249, 294, 478
AgsI TTSAA 3 cut(s) 271, 811, 1019
AjnI CCWGG 2 cut(s) 98, 214
AjuI GAANNNNNNNTTGG 2 cut(s) 711, 743
AleI CACNNNNGTG 1 cut(s) 567
AluBI AGCT 2 cut(s) 430, 826
AluI AGCT 2 cut(s) 430, 826
Alw21I GWGCWC 1 cut(s) 844
Alw26I GTCTC 3 cut(s) 273, 333, 923
AlwI GGATC 2 cut(s) 393, 406
AoxI GGCC 2 cut(s) 618, 972
ApeKI GCWGC 3 cut(s) 56, 734, 953
ApoI RAATTY 4 cut(s) 75, 266, 459, 1078
Asp700I GAANNNNTTC 2 cut(s) 536, 987
AspS9I GGNCC 1 cut(s) 372
AsuHPI GGTGA 2 cut(s) 88, 161
AvaII GGWCC 1 cut(s) 372
AxyI CCTNAGG 1 cut(s) 70
BamHI GGATCC 1 cut(s) 398
BarI GAAGNNNNNNTAC 2 cut(s) 125, 157
BauI CACGAG 1 cut(s) 336
BbsI GAAGAC 1 cut(s) 818
Bbv12I GWGCWC 1 cut(s) 844
BbvI GCAGC 3 cut(s) 43, 721, 965
BccI CCATC 7 cut(s) 45, 206, 215, 389, 746, 793, 812
BceAI ACGGC 2 cut(s) 605, 987
BciT130I CCWGG 2 cut(s) 100, 216
BciVI GTATCC 2 cut(s) 410, 736
BcoDI GTCTC 3 cut(s) 273, 333, 923
BfaI CTAG 2 cut(s) 60, 431
BfmI CTRYAG 1 cut(s) 27
BfuI GTATCC 2 cut(s) 410, 736
BglII AGATCT 1 cut(s) 163
BisI GCNGC 3 cut(s) 57, 735, 954
BlsI GCNGC 3 cut(s) 58, 736, 955
Bme1390I CCNGG 2 cut(s) 100, 216
Bme18I GGWCC 1 cut(s) 372
BmgT120I GGNCC 1 cut(s) 372
BmiI GGNNCC 1 cut(s) 400
BmrFI CCNGG 2 cut(s) 100, 216
BpiI GAAGAC 1 cut(s) 818
Bpu10I CCTNAGC 2 cut(s) 177, 589
BpuEI CTTGAG 1 cut(s) 918
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 3 cut(s) 214, 615, 621
BsaWI WCCGGW 1 cut(s) 242
Bsc4I CCNNNNNNNGG 4 cut(s) 178, 249, 294, 478
Bse1I ACTGG 2 cut(s) 483, 866
Bse21I CCTNAGG 1 cut(s) 70
Bse3DI GCAATG 1 cut(s) 681
BseBI CCWGG 2 cut(s) 100, 216
BseDI CCNNGG 3 cut(s) 214, 615, 621
BseGI GGATG 3 cut(s) 56, 757, 823
BseLI CCNNNNNNNGG 4 cut(s) 178, 249, 294, 478
BseMI GCAATG 1 cut(s) 681
BseMII CTCAG 2 cut(s) 84, 174
BseNI ACTGG 2 cut(s) 483, 866
BseRI GAGGAG 2 cut(s) 455, 834
BseX3I CGGCCG 1 cut(s) 972
BseXI GCAGC 3 cut(s) 43, 721, 965
BseYI CCCAGC 1 cut(s) 506
BsgI GTGCAG 2 cut(s) 59, 316
Bsh1236I CGCG 1 cut(s) 714
Bsh1285I CGRYCG 1 cut(s) 975
BshFI GGCC 2 cut(s) 620, 974
BsiEI CGRYCG 1 cut(s) 975
BsiHKAI GWGCWC 1 cut(s) 844
BsiSI CCGG 2 cut(s) 243, 288
BslFI GGGAC 1 cut(s) 197
BslI CCNNNNNNNGG 4 cut(s) 178, 249, 294, 478
BsmAI GTCTC 3 cut(s) 273, 333, 923
BsmFI GGGAC 1 cut(s) 197
BsnI GGCC 2 cut(s) 620, 974
Bsp1286I GDGCHC 1 cut(s) 844
Bsp1407I TGTACA 1 cut(s) 519
Bsp143I GATC 2 cut(s) 163, 398
Bsp68I TCGCGA 1 cut(s) 714
BspACI CCGC 1 cut(s) 1048
BspANI GGCC 2 cut(s) 620, 974
BspCNI CTCAG 2 cut(s) 83, 173
BspFNI CGCG 1 cut(s) 714
BspLI GGNNCC 1 cut(s) 400
BspMAI CTGCAG 1 cut(s) 31
BspPI GGATC 2 cut(s) 393, 406
BsrDI GCAATG 1 cut(s) 681
BsrGI TGTACA 1 cut(s) 519
BsrI ACTGG 2 cut(s) 483, 866
BssECI CCNNGG 3 cut(s) 214, 615, 621
BssMI GATC 2 cut(s) 163, 398
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 336
BssT1I CCWWGG 1 cut(s) 615
Bst2BI CACGAG 1 cut(s) 336
Bst2UI CCWGG 2 cut(s) 100, 216
Bst4CI ACNGT 5 cut(s) 19, 146, 517, 640, 769
BstACI GRCGYC 1 cut(s) 5
BstAUI TGTACA 1 cut(s) 519
BstC8I GCNNGC 1 cut(s) 42
BstDEI CTNAG 4 cut(s) 70, 160, 177, 589
BstDSI CCRYGG 1 cut(s) 621
BstENI CCTNNNNNAGG 1 cut(s) 176
BstF5I GGATG 3 cut(s) 56, 757, 823
BstFNI CGCG 1 cut(s) 714
BstKTI GATC 2 cut(s) 166, 401
BstMAI GTCTC 3 cut(s) 273, 333, 923
BstMBI GATC 2 cut(s) 163, 398
BstMCI CGRYCG 1 cut(s) 975
BstMWI GCNNNNNNNGC 2 cut(s) 559, 908
BstNI CCWGG 2 cut(s) 100, 216
BstSCI CCNGG 2 cut(s) 98, 214
BstSFI CTRYAG 1 cut(s) 27
BstUI CGCG 1 cut(s) 714
BstV1I GCAGC 3 cut(s) 43, 721, 965
BstV2I GAAGAC 1 cut(s) 818
BstX2I RGATCY 2 cut(s) 163, 398
BstXI CCANNNNNNTGG 1 cut(s) 622
BstYI RGATCY 2 cut(s) 163, 398
BstZI CGGCCG 1 cut(s) 972
Bsu36I CCTNAGG 1 cut(s) 70
BsuI GTATCC 2 cut(s) 410, 736
BsuRI GGCC 2 cut(s) 620, 974
BtgI CCRYGG 1 cut(s) 621
BtsCI GGATG 3 cut(s) 56, 757, 823
BtsI GCAGTG 1 cut(s) 1008
BtsIMutI CAGTG 4 cut(s) 476, 636, 873, 1008
BtuMI TCGCGA 1 cut(s) 714
Cac8I GCNNGC 1 cut(s) 42
Cfr13I GGNCC 1 cut(s) 372
Csp6I GTAC 4 cut(s) 324, 520, 648, 1045
CviAII CATG 5 cut(s) 130, 221, 301, 554, 883
CviQI GTAC 4 cut(s) 324, 520, 648, 1045
DdeI CTNAG 4 cut(s) 70, 160, 177, 589
DpnI GATC 2 cut(s) 165, 400
DpnII GATC 2 cut(s) 163, 398
EaeI YGGCCR 1 cut(s) 972
EagI CGGCCG 1 cut(s) 972
EclXI CGGCCG 1 cut(s) 972
Eco130I CCWWGG 1 cut(s) 615
Eco32I GATATC 1 cut(s) 124
Eco47I GGWCC 1 cut(s) 372
Eco52I CGGCCG 1 cut(s) 972
Eco57I CTGAAG 1 cut(s) 30
Eco81I CCTNAGG 1 cut(s) 70
EcoNI CCTNNNNNAGG 1 cut(s) 176
EcoRI GAATTC 2 cut(s) 75, 459
EcoRII CCWGG 2 cut(s) 98, 214
EcoRV GATATC 1 cut(s) 124
EcoT14I CCWWGG 1 cut(s) 615
ErhI CCWWGG 1 cut(s) 615
FaeI CATG 5 cut(s) 133, 224, 304, 557, 886
FalI AAGNNNNNCTT 2 cut(s) 1034, 1066
FaqI GGGAC 1 cut(s) 197
FatI CATG 5 cut(s) 129, 220, 300, 553, 882
FauNDI CATATG 1 cut(s) 46
Fnu4HI GCNGC 3 cut(s) 57, 735, 954
FokI GGATG 3 cut(s) 63, 764, 830
Fsp4HI GCNGC 3 cut(s) 57, 735, 954
FspBI CTAG 2 cut(s) 60, 431
GluI GCNGC 3 cut(s) 57, 735, 954
GsaI CCCAGC 1 cut(s) 510
HaeIII GGCC 2 cut(s) 620, 974
HapII CCGG 2 cut(s) 243, 288
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 5 cut(s) 133, 224, 304, 557, 886
HincII GTYRAC 1 cut(s) 694
HindII GTYRAC 1 cut(s) 694
HinfI GANTC 2 cut(s) 65, 931
HpaII CCGG 2 cut(s) 243, 288
HphI GGTGA 2 cut(s) 88, 161
Hpy166II GTNNAC 4 cut(s) 324, 520, 694, 1006
Hpy188I TCNGA 7 cut(s) 10, 163, 191, 233, 424, 458, 542
Hpy188III TCNNGA 7 cut(s) 72, 402, 464, 713, 857, 935, 1016
Hpy8I GTNNAC 4 cut(s) 324, 520, 694, 1006
HpyAV CCTTC 3 cut(s) 97, 476, 661
HpyCH4III ACNGT 5 cut(s) 19, 146, 517, 640, 769
HpyCH4IV ACGT 3 cut(s) 5, 258, 407
HpyCH4V TGCA 9 cut(s) 29, 40, 300, 333, 491, 850, 911, 953, 1067
HpyF10VI GCNNNNNNNGC 2 cut(s) 559, 908
HpyF3I CTNAG 4 cut(s) 70, 160, 177, 589
HpySE526I ACGT 3 cut(s) 5, 258, 407
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 5 cut(s) 133, 224, 304, 557, 886
Kzo9I GATC 2 cut(s) 163, 398
LmnI GCTCC 3 cut(s) 252, 442, 847
Lsp1109I GCAGC 3 cut(s) 43, 721, 965
MaeI CTAG 2 cut(s) 60, 431
MaeII ACGT 3 cut(s) 5, 258, 407
MaeIII GTNAC 4 cut(s) 238, 259, 474, 634
MalI GATC 2 cut(s) 165, 400
MboI GATC 2 cut(s) 163, 398
MboII GAAGA 4 cut(s) 145, 158, 676, 823
MfeI CAATTG 1 cut(s) 912
MflI RGATCY 2 cut(s) 163, 398
MhlI GDGCHC 1 cut(s) 844
MluCI AATT 8 cut(s) 75, 266, 349, 459, 725, 828, 912, 1078
MlyI GAGTC 1 cut(s) 925
MmeI TCCRAC 3 cut(s) 169, 211, 447
MnlI CCTC 9 cut(s) 25, 79, 96, 433, 661, 789, 855, 864, 882
MroXI GAANNNNTTC 2 cut(s) 536, 987
MseI TTAA 2 cut(s) 606, 878
MslI CAYNNNNRTG 3 cut(s) 49, 567, 992
MspI CCGG 2 cut(s) 243, 288
MspR9I CCNGG 2 cut(s) 100, 216
MunI CAATTG 1 cut(s) 912
MvaI CCWGG 2 cut(s) 100, 216
MvnI CGCG 1 cut(s) 714
MwoI GCNNNNNNNGC 2 cut(s) 559, 908
NdeI CATATG 1 cut(s) 46
NdeII GATC 2 cut(s) 163, 398
NlaIII CATG 5 cut(s) 133, 224, 304, 557, 886
NlaIV GGNNCC 1 cut(s) 400
NmuCI GTSAC 4 cut(s) 238, 259, 474, 634
NruI TCGCGA 1 cut(s) 714
OliI CACNNNNGTG 1 cut(s) 567
PcsI WCGNNNNNNNCGW 2 cut(s) 317, 693
PdmI GAANNNNTTC 2 cut(s) 536, 987
PfeI GAWTC 1 cut(s) 65
PflFI GACNNNGTC 1 cut(s) 185
PkrI GCNGC 3 cut(s) 58, 736, 955
PleI GAGTC 1 cut(s) 925
PpsI GAGTC 1 cut(s) 925
Psp1406I AACGTT 1 cut(s) 407
Psp6I CCWGG 2 cut(s) 98, 214
PspFI CCCAGC 1 cut(s) 506
PspGI CCWGG 2 cut(s) 98, 214
PspN4I GGNNCC 1 cut(s) 400
PspPI GGNCC 1 cut(s) 372
PstI CTGCAG 1 cut(s) 31
PsuI RGATCY 2 cut(s) 163, 398
PsyI GACNNNGTC 1 cut(s) 185
RruI TCGCGA 1 cut(s) 714
RsaI GTAC 4 cut(s) 325, 521, 649, 1046
RsaNI GTAC 4 cut(s) 324, 520, 648, 1045
RseI CAYNNNNRTG 3 cut(s) 49, 567, 992
SaqAI TTAA 2 cut(s) 606, 878
SatI GCNGC 3 cut(s) 57, 735, 954
Sau3AI GATC 2 cut(s) 163, 398
Sau96I GGNCC 1 cut(s) 372
SchI GAGTC 1 cut(s) 925
ScrFI CCNGG 2 cut(s) 100, 216
SduI GDGCHC 1 cut(s) 844
SfcI CTRYAG 1 cut(s) 27
SinI GGWCC 1 cut(s) 372
SmiMI CAYNNNNRTG 3 cut(s) 49, 567, 992
SmlI CTYRAG 1 cut(s) 933
SmoI CTYRAG 1 cut(s) 933
Sse9I AATT 8 cut(s) 75, 266, 349, 459, 725, 828, 912, 1078
SsiI CCGC 1 cut(s) 1048
SspMI CTAG 2 cut(s) 60, 431
StyD4I CCNGG 2 cut(s) 98, 214
StyI CCWWGG 1 cut(s) 615
TaaI ACNGT 5 cut(s) 19, 146, 517, 640, 769
TaiI ACGT 3 cut(s) 8, 261, 410
TaqI TCGA 3 cut(s) 438, 762, 981
TasI AATT 8 cut(s) 75, 266, 349, 459, 725, 828, 912, 1078
TatI WGTACW 2 cut(s) 323, 519
TfiI GAWTC 1 cut(s) 65
Tru1I TTAA 2 cut(s) 606, 878
Tru9I TTAA 2 cut(s) 606, 878
TscAI CASTG 4 cut(s) 483, 643, 873, 1015
TseFI GTSAC 4 cut(s) 238, 259, 474, 634
TseI GCWGC 3 cut(s) 56, 734, 953
Tsp45I GTSAC 4 cut(s) 238, 259, 474, 634
TspDTI ATGAA 4 cut(s) 146, 545, 644, 938
TspGWI ACGGA 1 cut(s) 830
TspRI CASTG 4 cut(s) 483, 643, 873, 1015
Tth111I GACNNNGTC 1 cut(s) 185
VpaK11BI GGWCC 1 cut(s) 372
XagI CCTNNNNNAGG 1 cut(s) 176
XapI RAATTY 4 cut(s) 75, 266, 459, 1078
XmnI GAANNNNTTC 2 cut(s) 536, 987
XspI CTAG 2 cut(s) 60, 431
ZraI GACGTC 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.