Rh2BG230200

Dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0002881
Physical Location & Seq
Forward (+)
22642839 .. 22646220
3382 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG230200.1

Sequence Viewer

Length: 786 bp
ATGAAGACATATAACAAATACAAAGAGAAAATGCCTAAAGCACCAGCTCAAGAAGAAGCAGTGCATGCTTATGGATGGGCTGCTAGAGATTCCTCAGGAATTCTTTCCCCCTACCATTTCACTAGAAGGGCTAACGGGGATAATGATATCACCATCAAGATACAATATTGCGGAATTTGCCATGGAGACATTCACCTCGTAAAGAACAAAGTATGGTCGACCATTTATCCGACTGTGCCCGGGCATGAAATTGTTGGCCAAGTGACCAAGGTTGGGCTCAACGTCAAAAAATTCAAAGTTGGAGATGTAGCCGGAGTAGGGTGCATGATGGGTTCATGTGGCTCATGCGAGAACTGCAAACAAGACTTGGAAAATTACTGCCCCAAAATGTTATGGACCTTCAGTGGACAATATGAAGATGGAACAAGAGCCTTTGGTGGATATTCAGATAAGTTTGTTGTGGAAGAGCACTTTGCCGTCCGAATCCCAAATGACATTCCACTAGCAGGTGCCGCCCCGCTATTGTGTGCTGGGATTACCGTGTATAGCCCCCTGAAATATTTCGGACTAGTCCCCCAGCCCGGTAGCAAGAAACATTTAGGTGTTGTTGGGCTTGGAGGTGTAGGTCATATGGCCGTGAAGTTTGCCAAAGCTTTCGGAGCAAAGGTCACTGTGATTAGTTCCTCTCCGAGTAAGGAAAAGGAAGCAGTGGAACGACTTGGTGCAGATTCATTTTTGATCAGTCATGACCATGAGAAATTGGAGGTAATTAAGTACCGAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
ADH_N ADH_zinc_N

Protein Analysis

261

Amino Acids

28.49

Weight (kDa)

8.88

Isoelectric Point (pI)

34.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 46 - 163 6.1e-25 Alcohol dehydrogenase GroES-like domain
2-Hacid_dh_C PF02826 196 - 239 5e-06 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
ADH_zinc_N PF00107 207 - 250 6.7e-09 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 497
Acc36I ACCTGC 1 cut(s) 497
AccB1I GGYRCC 1 cut(s) 509
AccI GTMKAC 1 cut(s) 218
AciI CCGC 3 cut(s) 171, 513, 518
AcoI YGGCCR 2 cut(s) 256, 633
AcsI RAATTY 3 cut(s) 99, 174, 290
AcuI CTGAAG 1 cut(s) 385
AfaI GTAC 1 cut(s) 776
AfiI CCNNNNNNNGG 4 cut(s) 273, 318, 506, 581
AgsI TTSAA 1 cut(s) 295
AhlI ACTAGT 1 cut(s) 568
AluBI AGCT 2 cut(s) 47, 653
AluI AGCT 2 cut(s) 47, 653
Alw21I GWGCWC 1 cut(s) 471
Alw26I GTCTC 1 cut(s) 180
Ama87I CYCGRG 1 cut(s) 239
AoxI GGCC 2 cut(s) 256, 633
ApeKI GCWGC 1 cut(s) 80
ApoI RAATTY 3 cut(s) 99, 174, 290
Asp700I GAANNNNTTC 2 cut(s) 103, 560
AspS9I GGNCC 1 cut(s) 396
AsuC2I CCSGG 3 cut(s) 240, 241, 582
AsuHPI GGTGA 2 cut(s) 142, 185
AvaI CYCGRG 1 cut(s) 239
AvaII GGWCC 1 cut(s) 396
AxyI CCTNAGG 1 cut(s) 94
BaeGI GKGCMC 1 cut(s) 240
BalI TGGCCA 1 cut(s) 258
BanI GGYRCC 1 cut(s) 509
BanII GRGCYC 1 cut(s) 279
BbsI GAAGAC 1 cut(s) 11
Bbv12I GWGCWC 1 cut(s) 471
BbvI GCAGC 1 cut(s) 67
BccI CCATC 4 cut(s) 69, 161, 322, 413
BceAI ACGGC 2 cut(s) 461, 620
BclI TGATCA 1 cut(s) 738
BcnI CCSGG 3 cut(s) 240, 241, 582
BcoDI GTCTC 1 cut(s) 180
BcuI ACTAGT 1 cut(s) 568
BfaI CTAG 4 cut(s) 84, 123, 503, 569
BfuAI ACCTGC 1 cut(s) 497
BisI GCNGC 2 cut(s) 81, 513
BlsI GCNGC 2 cut(s) 82, 514
Bme1390I CCNGG 3 cut(s) 240, 241, 582
Bme18I GGWCC 1 cut(s) 396
BmeT110I CYCGRG 1 cut(s) 239
BmgT120I GGNCC 1 cut(s) 396
BmiI GGNNCC 1 cut(s) 511
BmrFI CCNGG 3 cut(s) 240, 241, 582
BpiI GAAGAC 1 cut(s) 11
BpuEI CTTGAG 1 cut(s) 33
BpuMI CCSGG 3 cut(s) 240, 241, 582
BsaJI CCNNGG 3 cut(s) 181, 239, 267
BsaXI ACNNNNNCTCC 2 cut(s) 651, 681
Bsc4I CCNNNNNNNGG 4 cut(s) 273, 318, 506, 581
Bse21I CCTNAGG 1 cut(s) 94
BseDI CCNNGG 3 cut(s) 181, 239, 267
BseGI GGATG 1 cut(s) 80
BseLI CCNNNNNNNGG 4 cut(s) 273, 318, 506, 581
BseMII CTCAG 1 cut(s) 108
BseSI GKGCMC 1 cut(s) 240
BseXI GCAGC 1 cut(s) 67
BseYI CCCAGC 2 cut(s) 530, 576
BsgI GTGCAG 1 cut(s) 744
BshFI GGCC 2 cut(s) 258, 635
BshNI GGYRCC 1 cut(s) 509
BsiHKAI GWGCWC 1 cut(s) 471
BsiHKCI CYCGRG 1 cut(s) 239
BsiSI CCGG 3 cut(s) 240, 312, 582
BslFI GGGAC 1 cut(s) 557
BslI CCNNNNNNNGG 4 cut(s) 273, 318, 506, 581
BsmAI GTCTC 1 cut(s) 180
BsmFI GGGAC 1 cut(s) 557
BsnI GGCC 2 cut(s) 258, 635
BsoBI CYCGRG 1 cut(s) 239
Bsp1286I GDGCHC 3 cut(s) 240, 279, 471
Bsp143I GATC 1 cut(s) 738
Bsp19I CCATGG 1 cut(s) 181
BspACI CCGC 3 cut(s) 171, 513, 518
BspANI GGCC 2 cut(s) 258, 635
BspCNI CTCAG 1 cut(s) 107
BspHI TCATGA 1 cut(s) 745
BspLI GGNNCC 1 cut(s) 511
BspMI ACCTGC 1 cut(s) 497
BspQI GCTCTTC 1 cut(s) 459
BspT107I GGYRCC 1 cut(s) 509
BssECI CCNNGG 3 cut(s) 181, 239, 267
BssMI GATC 1 cut(s) 738
BssT1I CCWWGG 2 cut(s) 181, 267
Bst4CI ACNGT 3 cut(s) 235, 541, 673
Bst6I CTCTTC 1 cut(s) 459
BstAPI GCANNNNNTGC 1 cut(s) 65
BstC8I GCNNGC 1 cut(s) 66
BstDEI CTNAG 1 cut(s) 94
BstDSI CCRYGG 1 cut(s) 181
BstF5I GGATG 1 cut(s) 80
BstKTI GATC 1 cut(s) 741
BstMAI GTCTC 1 cut(s) 180
BstMBI GATC 1 cut(s) 738
BstMWI GCNNNNNNNGC 5 cut(s) 65, 177, 354, 512, 659
BstNSI RCATGY 1 cut(s) 68
BstSCI CCNGG 3 cut(s) 238, 239, 580
BstSLI GKGCMC 1 cut(s) 240
BstV1I GCAGC 1 cut(s) 67
BstV2I GAAGAC 1 cut(s) 11
Bsu36I CCTNAGG 1 cut(s) 94
BsuRI GGCC 2 cut(s) 258, 635
BtgI CCRYGG 1 cut(s) 181
BtsCI GGATG 1 cut(s) 80
BtsI GCAGTG 2 cut(s) 66, 714
BtsIMutI CAGTG 4 cut(s) 66, 409, 669, 714
BveI ACCTGC 1 cut(s) 497
Cac8I GCNNGC 1 cut(s) 66
CciI TCATGA 1 cut(s) 745
Cfr13I GGNCC 1 cut(s) 396
Cfr9I CCCGGG 1 cut(s) 239
Csp6I GTAC 1 cut(s) 775
CviAII CATG 8 cut(s) 65, 182, 245, 325, 336, 345, 746, 752
CviQI GTAC 1 cut(s) 775
DdeI CTNAG 1 cut(s) 94
DpnI GATC 1 cut(s) 740
DpnII GATC 1 cut(s) 738
EaeI YGGCCR 2 cut(s) 256, 633
Eam1104I CTCTTC 1 cut(s) 459
EarI CTCTTC 1 cut(s) 459
Eco130I CCWWGG 2 cut(s) 181, 267
Eco24I GRGCYC 1 cut(s) 279
Eco32I GATATC 1 cut(s) 148
Eco47I GGWCC 1 cut(s) 396
Eco57I CTGAAG 1 cut(s) 385
Eco81I CCTNAGG 1 cut(s) 94
Eco88I CYCGRG 1 cut(s) 239
EcoRI GAATTC 1 cut(s) 99
EcoRV GATATC 1 cut(s) 148
EcoT14I CCWWGG 2 cut(s) 181, 267
EcoT38I GRGCYC 1 cut(s) 279
ErhI CCWWGG 2 cut(s) 181, 267
FaeI CATG 8 cut(s) 68, 185, 248, 328, 339, 348, 749, 755
FaqI GGGAC 1 cut(s) 557
FatI CATG 8 cut(s) 64, 181, 244, 324, 335, 344, 745, 751
FauI CCCGC 1 cut(s) 525
FauNDI CATATG 1 cut(s) 630
FbaI TGATCA 1 cut(s) 738
FblI GTMKAC 1 cut(s) 218
Fnu4HI GCNGC 2 cut(s) 81, 513
FokI GGATG 1 cut(s) 87
FriOI GRGCYC 1 cut(s) 279
Fsp4HI GCNGC 2 cut(s) 81, 513
FspBI CTAG 4 cut(s) 84, 123, 503, 569
GluI GCNGC 2 cut(s) 81, 513
GsaI CCCAGC 2 cut(s) 534, 580
HaeIII GGCC 2 cut(s) 258, 635
HapII CCGG 3 cut(s) 240, 312, 582
Hin1II CATG 8 cut(s) 68, 185, 248, 328, 339, 348, 749, 755
HincII GTYRAC 1 cut(s) 219
HindII GTYRAC 1 cut(s) 219
HindIII AAGCTT 1 cut(s) 651
HinfI GANTC 3 cut(s) 89, 483, 728
HpaII CCGG 3 cut(s) 240, 312, 582
HphI GGTGA 2 cut(s) 142, 185
Hpy166II GTNNAC 2 cut(s) 219, 407
Hpy188I TCNGA 6 cut(s) 231, 448, 482, 566, 659, 690
Hpy188III TCNNGA 4 cut(s) 50, 96, 157, 746
Hpy8I GTNNAC 2 cut(s) 219, 407
HpyAV CCTTC 2 cut(s) 120, 409
HpyCH4III ACNGT 3 cut(s) 235, 541, 673
HpyCH4IV ACGT 1 cut(s) 282
HpyCH4V TGCA 4 cut(s) 64, 324, 357, 725
HpyF10VI GCNNNNNNNGC 5 cut(s) 65, 177, 354, 512, 659
HpyF3I CTNAG 1 cut(s) 94
HpySE526I ACGT 1 cut(s) 282
Hsp92II CATG 8 cut(s) 68, 185, 248, 328, 339, 348, 749, 755
Ksp22I TGATCA 1 cut(s) 738
Kzo9I GATC 1 cut(s) 738
LguI GCTCTTC 1 cut(s) 459
LmnI GCTCC 1 cut(s) 659
LpnPI CCDG 9 cut(s) 57, 81, 253, 325, 492, 516, 566, 590, 595
Lsp1109I GCAGC 1 cut(s) 67
MaeI CTAG 4 cut(s) 84, 123, 503, 569
MaeII ACGT 1 cut(s) 282
MaeIII GTNAC 2 cut(s) 262, 667
MalI GATC 1 cut(s) 740
MboI GATC 1 cut(s) 738
MboII GAAGA 4 cut(s) 16, 65, 428, 476
MhlI GDGCHC 3 cut(s) 240, 279, 471
MlsI TGGCCA 1 cut(s) 258
MluCI AATT 7 cut(s) 99, 174, 249, 290, 373, 758, 768
MluNI TGGCCA 1 cut(s) 258
MmeI TCCRAC 2 cut(s) 254, 280
MnlI CCTC 5 cut(s) 103, 206, 611, 694, 757
Mox20I TGGCCA 1 cut(s) 258
MroXI GAANNNNTTC 2 cut(s) 103, 560
MscI TGGCCA 1 cut(s) 258
MseI TTAA 2 cut(s) 771, 784
MslI CAYNNNNRTG 3 cut(s) 69, 600, 750
Msp20I TGGCCA 1 cut(s) 258
MspI CCGG 3 cut(s) 240, 312, 582
MspR9I CCNGG 3 cut(s) 240, 241, 582
MwoI GCNNNNNNNGC 5 cut(s) 65, 177, 354, 512, 659
NciI CCSGG 3 cut(s) 240, 241, 582
NcoI CCATGG 1 cut(s) 181
NdeI CATATG 1 cut(s) 630
NdeII GATC 1 cut(s) 738
NlaIII CATG 8 cut(s) 68, 185, 248, 328, 339, 348, 749, 755
NlaIV GGNNCC 1 cut(s) 511
NmuCI GTSAC 2 cut(s) 262, 667
NspI RCATGY 1 cut(s) 68
PaeI GCATGC 1 cut(s) 68
PagI TCATGA 1 cut(s) 745
PaqCI CACCTGC 1 cut(s) 497
PciSI GCTCTTC 1 cut(s) 459
PdmI GAANNNNTTC 2 cut(s) 103, 560
PfeI GAWTC 3 cut(s) 89, 483, 728
PkrI GCNGC 2 cut(s) 82, 514
PspFI CCCAGC 2 cut(s) 530, 576
PspN4I GGNNCC 1 cut(s) 511
PspPI GGNCC 1 cut(s) 396
RsaI GTAC 1 cut(s) 776
RsaNI GTAC 1 cut(s) 775
RseI CAYNNNNRTG 3 cut(s) 69, 600, 750
SalI GTCGAC 1 cut(s) 217
SapI GCTCTTC 1 cut(s) 459
SaqAI TTAA 2 cut(s) 771, 784
SatI GCNGC 2 cut(s) 81, 513
Sau3AI GATC 1 cut(s) 738
Sau96I GGNCC 1 cut(s) 396
ScrFI CCNGG 3 cut(s) 240, 241, 582
SduI GDGCHC 3 cut(s) 240, 279, 471
SinI GGWCC 1 cut(s) 396
SmaI CCCGGG 1 cut(s) 241
SmiMI CAYNNNNRTG 3 cut(s) 69, 600, 750
SmlI CTYRAG 1 cut(s) 48
SmoI CTYRAG 1 cut(s) 48
SpeI ACTAGT 1 cut(s) 568
SphI GCATGC 1 cut(s) 68
SrfI GCCCGGGC 1 cut(s) 241
Sse9I AATT 7 cut(s) 99, 174, 249, 290, 373, 758, 768
SsiI CCGC 3 cut(s) 171, 513, 518
SspI AATATT 2 cut(s) 167, 560
SspMI CTAG 4 cut(s) 84, 123, 503, 569
StyD4I CCNGG 3 cut(s) 238, 239, 580
StyI CCWWGG 2 cut(s) 181, 267
TaaI ACNGT 3 cut(s) 235, 541, 673
TaiI ACGT 1 cut(s) 285
TaqI TCGA 1 cut(s) 218
TasI AATT 7 cut(s) 99, 174, 249, 290, 373, 758, 768
TauI GCSGC 1 cut(s) 515
TfiI GAWTC 3 cut(s) 89, 483, 728
Tru1I TTAA 2 cut(s) 771, 784
Tru9I TTAA 2 cut(s) 771, 784
TscAI CASTG 4 cut(s) 66, 409, 676, 714
TseFI GTSAC 2 cut(s) 262, 667
TseI GCWGC 1 cut(s) 80
Tsp45I GTSAC 2 cut(s) 262, 667
TspDTI ATGAA 5 cut(s) 17, 261, 324, 429, 720
TspMI CCCGGG 1 cut(s) 239
TspRI CASTG 4 cut(s) 66, 409, 676, 714
VpaK11BI GGWCC 1 cut(s) 396
XapI RAATTY 3 cut(s) 99, 174, 290
XceI RCATGY 1 cut(s) 68
XmaI CCCGGG 1 cut(s) 239
XmiI GTMKAC 1 cut(s) 218
XmnI GAANNNNTTC 2 cut(s) 103, 560
XspI CTAG 4 cut(s) 84, 123, 503, 569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.