Rh2AG165700

Dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0002881
Physical Location & Seq
Reverse (-)
15591263 .. 15593357
2095 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG165700.1

Sequence Viewer

Length: 1098 bp
ATGTCTGAAGCAATTATATCATCTGCAGAAGAACTGCAGGCAGTGAATGCCTACGGATGGGCTGCTAGAGATTCCTCGGGAATTCTCTCTCCCTTCCACTTCACCAGGAGGACTACTGGTAGGAATGATATCAGCATGAAGATACTTTACTGTGGCATTTGCCACTCAGACCTTCACCTTGCTAAGGACGAAGTTGCAATGACCATCTATCCCCTTGTCCCAGGACATGAGATTGTCGGGGAAGTGACCGAAGTCGGGAGCAACGTCACGAAATTTAAAGTTGGAGACATAGCCGGAGTGGGGTGCATGGTGGGTTCATGTGGTTCGTGCGGTAACTGCACACGAGATTTGGAAAATTACTGTCCCAAAGTGGTATGGACCTACAACAAACACTACCATGACGGATCGATAACTTTCGGTGGATACTCCGACAAATTTGTAGTCGAGGAGCATTTCGCAGTACGAATCCCAGATAACTTGCCATTGGAGGGGGTTGCACCTATGCTTTGTGCTGGCATTACGGTGTACAGCCCTATGAGACACTTTGGACTACTTGAGCATGGTAAGCATCTGGGTGTGGTAGGGCTTGGGGGCCTCGGTCATATGGCAGTGAAGTTTGCCAAGGCTATCGGAGTAAAAGTGACTGTGATCAGTACCTCTCCAAACAAGAAGAAGGAAGCAATGGAACAACTTGGTGTTGACCATTTTCTGATCAGTCGCAACCAAGAGGAAATGCAGGCTGCCATGGGTACGTTGGACGGTATCATCGATACAGTTTCAGCTCCTCATTCTCTCACGCCATTGGTTGACTTATTAACGACGGATGGAAAGCTAATCGTGGTGGGTGCTCCGCTCCAACCACCTGAGCTACCGGTGTTTCCTTTGATACTTGGGAGAAAGTCTATTGCCGGGAGTGCAATTGGGGGAATGAAAGAGACTCAAGAGATGATAGATTTTGCAGCAAAGCATAATATCACAGCTGATGTTGAAGTCATTCCTATGGATTATGTGAACACTGCTTTTGAACGAGTTGCCAAGTTAGATGTTAAGTACCGATTTGTCATTGACGTCGCAAACACCCTGAATTCTCCATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
ADH_N ADH_zinc_N

Protein Analysis

365

Amino Acids

39.43

Weight (kDa)

5.86

Isoelectric Point (pI)

32.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 41 - 158 2.2e-23 Alcohol dehydrogenase GroES-like domain
AlaDh_PNT_C PF01262 192 - 268 6.6e-06 Alanine dehydrogenase/PNT, C-terminal domain
ADH_zinc_N PF00107 198 - 321 2e-25 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1071
AccBSI CCGCTC 1 cut(s) 853
AciI CCGC 2 cut(s) 330, 851
AclWI GGATC 1 cut(s) 412
AcsI RAATTY 4 cut(s) 81, 272, 434, 1084
AcuI CTGAAG 1 cut(s) 27
AcyI GRCGYC 1 cut(s) 1068
AfaI GTAC 5 cut(s) 462, 527, 655, 751, 1052
AfiI CCNNNNNNNGG 5 cut(s) 57, 184, 255, 300, 488
AgeI ACCGGT 1 cut(s) 871
AgsI TTSAA 2 cut(s) 989, 1025
AjnI CCWGG 2 cut(s) 104, 220
AluBI AGCT 4 cut(s) 782, 832, 868, 980
AluI AGCT 4 cut(s) 782, 832, 868, 980
Alw21I GWGCWC 1 cut(s) 850
Alw26I GTCTC 3 cut(s) 279, 532, 929
AlwI GGATC 1 cut(s) 412
Ama87I CYCGRG 1 cut(s) 76
AoxI GGCC 1 cut(s) 592
ApeKI GCWGC 3 cut(s) 62, 740, 959
ApoI RAATTY 4 cut(s) 81, 272, 434, 1084
ArsI GACNNNNNNTTYG 2 cut(s) 426, 458
AsiGI ACCGGT 1 cut(s) 871
Asp700I GAANNNNTTC 1 cut(s) 993
AspS9I GGNCC 2 cut(s) 378, 592
AsuC2I CCSGG 1 cut(s) 910
AsuHPI GGTGA 2 cut(s) 94, 167
AvaI CYCGRG 1 cut(s) 76
AvaII GGWCC 1 cut(s) 378
BarI GAAGNNNNNNTAC 2 cut(s) 131, 163
BauI CACGAG 1 cut(s) 342
Bbv12I GWGCWC 1 cut(s) 850
BbvI GCAGC 3 cut(s) 49, 727, 971
BccI CCATC 3 cut(s) 51, 212, 818
BciT130I CCWGG 2 cut(s) 106, 222
BciVI GTATCC 1 cut(s) 416
BclI TGATCA 2 cut(s) 648, 711
BcnI CCSGG 1 cut(s) 910
BcoDI GTCTC 3 cut(s) 279, 532, 929
BfaI CTAG 1 cut(s) 66
BfmI CTRYAG 2 cut(s) 24, 35
BfuI GTATCC 1 cut(s) 416
BisI GCNGC 3 cut(s) 63, 741, 960
BlsI GCNGC 3 cut(s) 64, 742, 961
Bme1390I CCNGG 3 cut(s) 106, 222, 910
Bme18I GGWCC 1 cut(s) 378
BmeT110I CYCGRG 1 cut(s) 76
BmgT120I GGNCC 2 cut(s) 378, 592
BmiI GGNNCC 1 cut(s) 593
BmrFI CCNGG 3 cut(s) 106, 222, 910
BmsI GCATC 1 cut(s) 577
BoxI GACNNNNGTC 1 cut(s) 251
Bpu10I CCTNAGC 2 cut(s) 183, 864
BpuEI CTTGAG 2 cut(s) 575, 924
BpuMI CCSGG 1 cut(s) 910
Bsa29I ATCGAT 2 cut(s) 407, 768
BsaHI GRCGYC 1 cut(s) 1068
BsaJI CCNNGG 5 cut(s) 75, 220, 595, 621, 744
BsaWI WCCGGW 1 cut(s) 871
BsaXI ACNNNNNCTCC 2 cut(s) 624, 654
Bsc4I CCNNNNNNNGG 5 cut(s) 57, 184, 255, 300, 488
Bse118I RCCGGY 1 cut(s) 871
Bse1I ACTGG 1 cut(s) 121
Bse3DI GCAATG 2 cut(s) 204, 687
BseBI CCWGG 2 cut(s) 106, 222
BseCI ATCGAT 2 cut(s) 407, 768
BseDI CCNNGG 5 cut(s) 75, 220, 595, 621, 744
BseGI GGATG 2 cut(s) 62, 829
BseLI CCNNNNNNNGG 5 cut(s) 57, 184, 255, 300, 488
BseMI GCAATG 2 cut(s) 204, 687
BseMII CTCAG 2 cut(s) 180, 855
BseNI ACTGG 1 cut(s) 121
BseRI GAGGAG 2 cut(s) 461, 774
BseXI GCAGC 3 cut(s) 49, 727, 971
BsgI GTGCAG 1 cut(s) 322
BshFI GGCC 1 cut(s) 594
BshTI ACCGGT 1 cut(s) 871
BshVI ATCGAT 2 cut(s) 407, 768
BsiHKAI GWGCWC 1 cut(s) 850
BsiHKCI CYCGRG 1 cut(s) 76
BsiSI CCGG 3 cut(s) 294, 872, 909
BslFI GGGAC 2 cut(s) 203, 348
BslI CCNNNNNNNGG 5 cut(s) 57, 184, 255, 300, 488
BsmAI GTCTC 3 cut(s) 279, 532, 929
BsmFI GGGAC 2 cut(s) 203, 348
BsmI GAATGC 1 cut(s) 52
BsnI GGCC 1 cut(s) 594
BsoBI CYCGRG 1 cut(s) 76
Bsp1286I GDGCHC 1 cut(s) 850
Bsp1407I TGTACA 1 cut(s) 525
Bsp143I GATC 3 cut(s) 404, 648, 711
Bsp19I CCATGG 1 cut(s) 744
BspACI CCGC 2 cut(s) 330, 851
BspANI GGCC 1 cut(s) 594
BspCNI CTCAG 2 cut(s) 179, 856
BspDI ATCGAT 2 cut(s) 407, 768
BspLI GGNNCC 1 cut(s) 593
BspMAI CTGCAG 2 cut(s) 28, 39
BspPI GGATC 1 cut(s) 412
BsrBI CCGCTC 1 cut(s) 853
BsrDI GCAATG 2 cut(s) 204, 687
BsrFI RCCGGY 1 cut(s) 871
BsrGI TGTACA 1 cut(s) 525
BsrI ACTGG 1 cut(s) 121
BssAI RCCGGY 1 cut(s) 871
BssECI CCNNGG 5 cut(s) 75, 220, 595, 621, 744
BssMI GATC 3 cut(s) 404, 648, 711
BssNI GRCGYC 1 cut(s) 1068
BssSI CACGAG 1 cut(s) 342
BssT1I CCWWGG 2 cut(s) 621, 744
Bst2BI CACGAG 1 cut(s) 342
Bst2UI CCWGG 2 cut(s) 106, 222
Bst4CI ACNGT 6 cut(s) 152, 362, 523, 646, 761, 775
BstACI GRCGYC 1 cut(s) 1068
BstAPI GCANNNNNTGC 1 cut(s) 47
BstAUI TGTACA 1 cut(s) 525
BstC8I GCNNGC 3 cut(s) 39, 514, 738
BstDEI CTNAG 3 cut(s) 166, 183, 864
BstDSI CCRYGG 1 cut(s) 744
BstENI CCTNNNNNAGG 1 cut(s) 182
BstF5I GGATG 2 cut(s) 62, 829
BstKTI GATC 3 cut(s) 407, 651, 714
BstMAI GTCTC 3 cut(s) 279, 532, 929
BstMBI GATC 3 cut(s) 404, 648, 711
BstMWI GCNNNNNNNGC 4 cut(s) 47, 336, 565, 914
BstNI CCWGG 2 cut(s) 106, 222
BstPAI GACNNNNGTC 1 cut(s) 251
BstSCI CCNGG 3 cut(s) 104, 220, 908
BstSFI CTRYAG 2 cut(s) 24, 35
BstV1I GCAGC 3 cut(s) 49, 727, 971
Bsu15I ATCGAT 2 cut(s) 407, 768
BsuI GTATCC 1 cut(s) 416
BsuRI GGCC 1 cut(s) 594
BsuTUI ATCGAT 2 cut(s) 407, 768
BtgI CCRYGG 1 cut(s) 744
BtsCI GGATG 2 cut(s) 62, 829
BtsI GCAGTG 3 cut(s) 48, 615, 1014
BtsIMutI CAGTG 3 cut(s) 48, 615, 1014
Cac8I GCNNGC 3 cut(s) 39, 514, 738
Cfr10I RCCGGY 1 cut(s) 871
Cfr13I GGNCC 2 cut(s) 378, 592
ClaI ATCGAT 2 cut(s) 407, 768
Csp6I GTAC 5 cut(s) 461, 526, 654, 750, 1051
CspAI ACCGGT 1 cut(s) 871
CviAII CATG 7 cut(s) 136, 227, 307, 318, 398, 560, 745
CviQI GTAC 5 cut(s) 461, 526, 654, 750, 1051
DdeI CTNAG 3 cut(s) 166, 183, 864
DpnI GATC 3 cut(s) 406, 650, 713
DpnII GATC 3 cut(s) 404, 648, 711
DraI TTTAAA 1 cut(s) 277
Eco130I CCWWGG 2 cut(s) 621, 744
Eco32I GATATC 1 cut(s) 130
Eco47I GGWCC 1 cut(s) 378
Eco57I CTGAAG 1 cut(s) 27
Eco88I CYCGRG 1 cut(s) 76
EcoNI CCTNNNNNAGG 1 cut(s) 182
EcoO109I RGGNCCY 1 cut(s) 592
EcoRI GAATTC 2 cut(s) 81, 1084
EcoRII CCWGG 2 cut(s) 104, 220
EcoRV GATATC 1 cut(s) 130
EcoT14I CCWWGG 2 cut(s) 621, 744
ErhI CCWWGG 2 cut(s) 621, 744
FaeI CATG 7 cut(s) 139, 230, 310, 321, 401, 563, 748
FaqI GGGAC 2 cut(s) 203, 348
FatI CATG 7 cut(s) 135, 226, 306, 317, 397, 559, 744
FauNDI CATATG 1 cut(s) 603
FbaI TGATCA 2 cut(s) 648, 711
Fnu4HI GCNGC 3 cut(s) 63, 741, 960
FokI GGATG 2 cut(s) 69, 836
Fsp4HI GCNGC 3 cut(s) 63, 741, 960
FspBI CTAG 1 cut(s) 66
GluI GCNGC 3 cut(s) 63, 741, 960
HaeIII GGCC 1 cut(s) 594
HapII CCGG 3 cut(s) 294, 872, 909
Hin1I GRCGYC 1 cut(s) 1068
Hin1II CATG 7 cut(s) 139, 230, 310, 321, 401, 563, 748
HincII GTYRAC 2 cut(s) 700, 808
HindII GTYRAC 2 cut(s) 700, 808
HinfI GANTC 3 cut(s) 71, 465, 937
HpaII CCGG 3 cut(s) 294, 872, 909
HphI GGTGA 2 cut(s) 94, 167
Hpy166II GTNNAC 4 cut(s) 526, 700, 808, 1012
Hpy188I TCNGA 5 cut(s) 7, 169, 430, 632, 711
Hpy188III TCNNGA 4 cut(s) 78, 256, 268, 941
Hpy8I GTNNAC 4 cut(s) 526, 700, 808, 1012
Hpy99I CGWCG 2 cut(s) 823, 1073
HpyAV CCTTC 3 cut(s) 103, 182, 667
HpyCH4III ACNGT 6 cut(s) 152, 362, 523, 646, 761, 775
HpyCH4IV ACGT 3 cut(s) 264, 752, 1068
HpyCH4V TGCA 9 cut(s) 26, 37, 197, 306, 339, 497, 736, 917, 959
HpyF10VI GCNNNNNNNGC 4 cut(s) 47, 336, 565, 914
HpyF3I CTNAG 3 cut(s) 166, 183, 864
HpySE526I ACGT 3 cut(s) 264, 752, 1068
Hsp92I GRCGYC 1 cut(s) 1068
Hsp92II CATG 7 cut(s) 139, 230, 310, 321, 401, 563, 748
Ksp22I TGATCA 2 cut(s) 648, 711
Kzo9I GATC 3 cut(s) 404, 648, 711
LmnI GCTCC 5 cut(s) 258, 448, 787, 853, 858
Lsp1109I GCAGC 3 cut(s) 49, 727, 971
LweI GCATC 1 cut(s) 577
MaeI CTAG 1 cut(s) 66
MaeII ACGT 3 cut(s) 264, 752, 1068
MaeIII GTNAC 4 cut(s) 244, 265, 332, 640
MalI GATC 3 cut(s) 406, 650, 713
MbiI CCGCTC 1 cut(s) 853
MboI GATC 3 cut(s) 404, 648, 711
MboII GAAGA 3 cut(s) 41, 151, 682
MfeI CAATTG 1 cut(s) 918
MhlI GDGCHC 1 cut(s) 850
MluCI AATT 7 cut(s) 12, 81, 272, 355, 434, 918, 1084
MlyI GAGTC 1 cut(s) 931
MmeI TCCRAC 4 cut(s) 262, 453, 735, 880
MnlI CCTC 8 cut(s) 85, 102, 439, 481, 605, 667, 721, 795
MroXI GAANNNNTTC 1 cut(s) 993
MseI TTAA 3 cut(s) 276, 815, 1047
MslI CAYNNNNRTG 4 cut(s) 396, 521, 573, 998
MspA1I CMGCKG 1 cut(s) 980
MspI CCGG 3 cut(s) 294, 872, 909
MspR9I CCNGG 3 cut(s) 106, 222, 910
MunI CAATTG 1 cut(s) 918
Mva1269I GAATGC 1 cut(s) 52
MvaI CCWGG 2 cut(s) 106, 222
MwoI GCNNNNNNNGC 4 cut(s) 47, 336, 565, 914
NciI CCSGG 1 cut(s) 910
NcoI CCATGG 1 cut(s) 744
NdeI CATATG 1 cut(s) 603
NdeII GATC 3 cut(s) 404, 648, 711
NlaIII CATG 7 cut(s) 139, 230, 310, 321, 401, 563, 748
NlaIV GGNNCC 1 cut(s) 593
NmuCI GTSAC 3 cut(s) 244, 265, 640
PcsI WCGNNNNNNNCGW 2 cut(s) 261, 765
PctI GAATGC 1 cut(s) 52
PdmI GAANNNNTTC 1 cut(s) 993
PfeI GAWTC 2 cut(s) 71, 465
PinAI ACCGGT 1 cut(s) 871
PkrI GCNGC 3 cut(s) 64, 742, 961
PleI GAGTC 1 cut(s) 931
PpsI GAGTC 1 cut(s) 931
PshAI GACNNNNGTC 1 cut(s) 251
Psp6I CCWGG 2 cut(s) 104, 220
PspGI CCWGG 2 cut(s) 104, 220
PspN4I GGNNCC 1 cut(s) 593
PspPI GGNCC 2 cut(s) 378, 592
PstI CTGCAG 2 cut(s) 28, 39
PvuII CAGCTG 1 cut(s) 980
RsaI GTAC 5 cut(s) 462, 527, 655, 751, 1052
RsaNI GTAC 5 cut(s) 461, 526, 654, 750, 1051
RseI CAYNNNNRTG 4 cut(s) 396, 521, 573, 998
SaqAI TTAA 3 cut(s) 276, 815, 1047
SatI GCNGC 3 cut(s) 63, 741, 960
Sau3AI GATC 3 cut(s) 404, 648, 711
Sau96I GGNCC 2 cut(s) 378, 592
SchI GAGTC 1 cut(s) 931
ScrFI CCNGG 3 cut(s) 106, 222, 910
SduI GDGCHC 1 cut(s) 850
SfaNI GCATC 1 cut(s) 577
SfcI CTRYAG 2 cut(s) 24, 35
SinI GGWCC 1 cut(s) 378
SmiMI CAYNNNNRTG 4 cut(s) 396, 521, 573, 998
SmlI CTYRAG 2 cut(s) 554, 939
SmoI CTYRAG 2 cut(s) 554, 939
Sse9I AATT 7 cut(s) 12, 81, 272, 355, 434, 918, 1084
SsiI CCGC 2 cut(s) 330, 851
SspMI CTAG 1 cut(s) 66
StyD4I CCNGG 3 cut(s) 104, 220, 908
StyI CCWWGG 2 cut(s) 621, 744
TaaI ACNGT 6 cut(s) 152, 362, 523, 646, 761, 775
TaiI ACGT 3 cut(s) 267, 755, 1071
TaqI TCGA 3 cut(s) 407, 444, 768
TaqII GACCGA 2 cut(s) 263, 587
TasI AATT 7 cut(s) 12, 81, 272, 355, 434, 918, 1084
TatI WGTACW 1 cut(s) 525
TfiI GAWTC 2 cut(s) 71, 465
Tru1I TTAA 3 cut(s) 276, 815, 1047
Tru9I TTAA 3 cut(s) 276, 815, 1047
TscAI CASTG 3 cut(s) 48, 615, 1021
TseFI GTSAC 3 cut(s) 244, 265, 640
TseI GCWGC 3 cut(s) 62, 740, 959
Tsp45I GTSAC 3 cut(s) 244, 265, 640
TspDTI ATGAA 3 cut(s) 152, 306, 944
TspGWI ACGGA 3 cut(s) 69, 417, 836
TspRI CASTG 3 cut(s) 48, 615, 1021
VpaK11BI GGWCC 1 cut(s) 378
XagI CCTNNNNNAGG 1 cut(s) 182
XapI RAATTY 4 cut(s) 81, 272, 434, 1084
XcmI CCANNNNNNNNNTGG 1 cut(s) 751
XmnI GAANNNNTTC 1 cut(s) 993
XspI CTAG 1 cut(s) 66
ZraI GACGTC 1 cut(s) 1069
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.