Rroxscaffold_2G00139450

Dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002881
Physical Location & Seq
Forward (+)
77843370 .. 77845119
1750 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00139450.1

Sequence Viewer

Length: 1086 bp
ATGTCTGAAGCAATTATATCATCTGCAGAAGAACTGCAGGCAGTGAATGCCTACGGATGGGCTGCTAGAGATTCCTCGGGAATTCTCTCTCCCTTCCACTTCACCAGGAGGACTACTGGTAGGAATGATATCAGCATGAAGATACTTTACTGTGGCATTTGCCACTCAGACCTTCACCTTGCTAAGGACGAAGTTGCAATGACCATCTATCCCCTTGTCCCAGGACATGAGATTGTCGGGGAAGTGACCGAAGTCGGGAGCAACATCACGAAATTTAAAGTTGGAGACATAGCCGGAGTGGGGTGCATGGTGGGTTCATGTGGTTCGTGCGGTAACTGCACACGAGACTTGGAAAATTACTGTCCCAAAAGGGTACGGACCTACAACGACGGATCGATAACTTTCGGTGGATACTCCGACAAATTTGTAGTCGAGGAGCATTTCGCAGTACGAATCCCAGATAACTTGCCATTGGAGGGTGTTGCACCTATGCTTTGTGCTGGCATTTCGGTGTACAGCCCTATGAGACACTTTGGACTACTTGAGCATGGTAAGCACTTGGGTGTGGTAGGGCTTGGGGGCCTCGGTCATATGGCAGTGAAGTTTGCCAAGGCTATCGGAGTAAAAGTGACTGTGATCAGTACATCTCCAAACAAGAAGAAGGAAGCAATGGAACAACTTGGTGTTGACCATTTTCTGATCAGTCGCAACCAAGAGGAAATGCAGGCTGCCATGGGTTCATTGGACGGTATCATCGATACGGTTTCAGCTCCTCATTCTCTCACGCCATTGGTTGACTTATTAATGACGGATGGAAAGCTAATCGTGGTGGGTGCTCGGCTCCAACCACCTGAGCTACCGCTGTTTCCTTTGATACTTGGGAGAAAGTCTATTGCCGGGAGTGCAATTGGGGGAATGAAAGAGACTCAAGAGATGATAGATTTTGCAGCAAAGCATAATATCACAGCTGATGTTGAAGTCATTCCTATGGATTATGTGAACACTGCTTTTGAACGAGTTGCCAAGTTAGATGTTAAATACCGATTTGTCATTGACGTGGCAAACACCCTAAATTCTCCATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
ADH_N ADH_zinc_N

Protein Analysis

361

Amino Acids

38.98

Weight (kDa)

5.95

Isoelectric Point (pI)

33.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 41 - 154 3.3e-23 Alcohol dehydrogenase GroES-like domain
AlaDh_PNT_C PF01262 188 - 264 6.6e-06 Alanine dehydrogenase/PNT, C-terminal domain
ADH_zinc_N PF00107 194 - 317 2.5e-24 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 330, 860
AclWI GGATC 1 cut(s) 400
AcsI RAATTY 4 cut(s) 81, 272, 422, 1072
AcuI CTGAAG 1 cut(s) 27
AfaI GTAC 4 cut(s) 375, 450, 515, 643
AfiI CCNNNNNNNGG 5 cut(s) 57, 184, 255, 300, 476
AgsI TTSAA 2 cut(s) 977, 1013
AjiI CACGTC 1 cut(s) 1057
AjnI CCWGG 2 cut(s) 104, 220
AleI CACNNNNGTG 1 cut(s) 561
AluBI AGCT 4 cut(s) 770, 820, 856, 968
AluI AGCT 4 cut(s) 770, 820, 856, 968
Alw21I GWGCWC 1 cut(s) 838
Alw26I GTCTC 4 cut(s) 279, 339, 520, 917
AlwI GGATC 1 cut(s) 400
Ama87I CYCGRG 1 cut(s) 76
AoxI GGCC 1 cut(s) 580
ApeKI GCWGC 3 cut(s) 62, 728, 947
ApoI RAATTY 4 cut(s) 81, 272, 422, 1072
ArsI GACNNNNNNTTYG 2 cut(s) 414, 446
AseI ATTAAT 1 cut(s) 803
Asp700I GAANNNNTTC 1 cut(s) 981
AspS9I GGNCC 2 cut(s) 378, 580
AsuC2I CCSGG 1 cut(s) 898
AsuHPI GGTGA 2 cut(s) 94, 167
AvaI CYCGRG 1 cut(s) 76
AvaII GGWCC 1 cut(s) 378
BarI GAAGNNNNNNTAC 2 cut(s) 131, 163
BauI CACGAG 1 cut(s) 342
Bbv12I GWGCWC 1 cut(s) 838
BbvI GCAGC 3 cut(s) 49, 715, 959
BccI CCATC 3 cut(s) 51, 212, 806
BciT130I CCWGG 2 cut(s) 106, 222
BciVI GTATCC 1 cut(s) 404
BclI TGATCA 2 cut(s) 636, 699
BcnI CCSGG 1 cut(s) 898
BcoDI GTCTC 4 cut(s) 279, 339, 520, 917
BfaI CTAG 1 cut(s) 66
BfmI CTRYAG 2 cut(s) 24, 35
BfuI GTATCC 1 cut(s) 404
BisI GCNGC 3 cut(s) 63, 729, 948
BlsI GCNGC 3 cut(s) 64, 730, 949
Bme1390I CCNGG 3 cut(s) 106, 222, 898
Bme18I GGWCC 1 cut(s) 378
BmeT110I CYCGRG 1 cut(s) 76
BmgBI CACGTC 1 cut(s) 1057
BmgT120I GGNCC 2 cut(s) 378, 580
BmiI GGNNCC 2 cut(s) 581, 842
BmrFI CCNGG 3 cut(s) 106, 222, 898
BoxI GACNNNNGTC 1 cut(s) 251
Bpu10I CCTNAGC 2 cut(s) 183, 852
BpuEI CTTGAG 2 cut(s) 563, 912
BpuMI CCSGG 1 cut(s) 898
Bsa29I ATCGAT 2 cut(s) 395, 756
BsaJI CCNNGG 5 cut(s) 75, 220, 583, 609, 732
BsaXI ACNNNNNCTCC 2 cut(s) 612, 642
Bsc4I CCNNNNNNNGG 5 cut(s) 57, 184, 255, 300, 476
Bse1I ACTGG 1 cut(s) 121
Bse3DI GCAATG 2 cut(s) 204, 675
BseBI CCWGG 2 cut(s) 106, 222
BseCI ATCGAT 2 cut(s) 395, 756
BseDI CCNNGG 5 cut(s) 75, 220, 583, 609, 732
BseGI GGATG 2 cut(s) 62, 817
BseLI CCNNNNNNNGG 5 cut(s) 57, 184, 255, 300, 476
BseMI GCAATG 2 cut(s) 204, 675
BseMII CTCAG 2 cut(s) 180, 843
BseNI ACTGG 1 cut(s) 121
BseRI GAGGAG 2 cut(s) 449, 762
BseXI GCAGC 3 cut(s) 49, 715, 959
BsgI GTGCAG 1 cut(s) 322
BshFI GGCC 1 cut(s) 582
BshVI ATCGAT 2 cut(s) 395, 756
BsiHKAI GWGCWC 1 cut(s) 838
BsiHKCI CYCGRG 1 cut(s) 76
BsiSI CCGG 2 cut(s) 294, 897
BslFI GGGAC 2 cut(s) 203, 348
BslI CCNNNNNNNGG 5 cut(s) 57, 184, 255, 300, 476
BsmAI GTCTC 4 cut(s) 279, 339, 520, 917
BsmFI GGGAC 2 cut(s) 203, 348
BsmI GAATGC 1 cut(s) 52
BsnI GGCC 1 cut(s) 582
BsoBI CYCGRG 1 cut(s) 76
Bsp1286I GDGCHC 1 cut(s) 838
Bsp1407I TGTACA 1 cut(s) 513
Bsp143I GATC 3 cut(s) 392, 636, 699
Bsp19I CCATGG 1 cut(s) 732
BspACI CCGC 2 cut(s) 330, 860
BspANI GGCC 1 cut(s) 582
BspCNI CTCAG 2 cut(s) 179, 844
BspDI ATCGAT 2 cut(s) 395, 756
BspLI GGNNCC 2 cut(s) 581, 842
BspMAI CTGCAG 2 cut(s) 28, 39
BspPI GGATC 1 cut(s) 400
BsrDI GCAATG 2 cut(s) 204, 675
BsrGI TGTACA 1 cut(s) 513
BsrI ACTGG 1 cut(s) 121
BssECI CCNNGG 5 cut(s) 75, 220, 583, 609, 732
BssMI GATC 3 cut(s) 392, 636, 699
BssSI CACGAG 1 cut(s) 342
BssT1I CCWWGG 2 cut(s) 609, 732
Bst2BI CACGAG 1 cut(s) 342
Bst2UI CCWGG 2 cut(s) 106, 222
Bst4CI ACNGT 5 cut(s) 152, 362, 634, 749, 763
BstAPI GCANNNNNTGC 1 cut(s) 47
BstAUI TGTACA 1 cut(s) 513
BstC8I GCNNGC 3 cut(s) 39, 502, 726
BstDEI CTNAG 3 cut(s) 166, 183, 852
BstDSI CCRYGG 1 cut(s) 732
BstENI CCTNNNNNAGG 1 cut(s) 182
BstF5I GGATG 2 cut(s) 62, 817
BstKTI GATC 3 cut(s) 395, 639, 702
BstMAI GTCTC 4 cut(s) 279, 339, 520, 917
BstMBI GATC 3 cut(s) 392, 636, 699
BstMWI GCNNNNNNNGC 4 cut(s) 47, 336, 553, 902
BstNI CCWGG 2 cut(s) 106, 222
BstPAI GACNNNNGTC 1 cut(s) 251
BstSCI CCNGG 3 cut(s) 104, 220, 896
BstSFI CTRYAG 2 cut(s) 24, 35
BstV1I GCAGC 3 cut(s) 49, 715, 959
Bsu15I ATCGAT 2 cut(s) 395, 756
BsuI GTATCC 1 cut(s) 404
BsuRI GGCC 1 cut(s) 582
BsuTUI ATCGAT 2 cut(s) 395, 756
BtgI CCRYGG 1 cut(s) 732
BtrI CACGTC 1 cut(s) 1057
BtsCI GGATG 2 cut(s) 62, 817
BtsI GCAGTG 3 cut(s) 48, 603, 1002
BtsIMutI CAGTG 3 cut(s) 48, 603, 1002
Cac8I GCNNGC 3 cut(s) 39, 502, 726
Cfr13I GGNCC 2 cut(s) 378, 580
ClaI ATCGAT 2 cut(s) 395, 756
Csp6I GTAC 4 cut(s) 374, 449, 514, 642
CviAII CATG 6 cut(s) 136, 227, 307, 318, 548, 733
CviQI GTAC 4 cut(s) 374, 449, 514, 642
DdeI CTNAG 3 cut(s) 166, 183, 852
DpnI GATC 3 cut(s) 394, 638, 701
DpnII GATC 3 cut(s) 392, 636, 699
DraI TTTAAA 1 cut(s) 277
Eco130I CCWWGG 2 cut(s) 609, 732
Eco32I GATATC 1 cut(s) 130
Eco47I GGWCC 1 cut(s) 378
Eco57I CTGAAG 1 cut(s) 27
Eco88I CYCGRG 1 cut(s) 76
EcoNI CCTNNNNNAGG 1 cut(s) 182
EcoO109I RGGNCCY 1 cut(s) 580
EcoRI GAATTC 1 cut(s) 81
EcoRII CCWGG 2 cut(s) 104, 220
EcoRV GATATC 1 cut(s) 130
EcoT14I CCWWGG 2 cut(s) 609, 732
ErhI CCWWGG 2 cut(s) 609, 732
FaeI CATG 6 cut(s) 139, 230, 310, 321, 551, 736
FaqI GGGAC 2 cut(s) 203, 348
FatI CATG 6 cut(s) 135, 226, 306, 317, 547, 732
FauNDI CATATG 1 cut(s) 591
FbaI TGATCA 2 cut(s) 636, 699
Fnu4HI GCNGC 3 cut(s) 63, 729, 948
FokI GGATG 2 cut(s) 69, 824
Fsp4HI GCNGC 3 cut(s) 63, 729, 948
FspBI CTAG 1 cut(s) 66
GluI GCNGC 3 cut(s) 63, 729, 948
HaeIII GGCC 1 cut(s) 582
HapII CCGG 2 cut(s) 294, 897
Hin1II CATG 6 cut(s) 139, 230, 310, 321, 551, 736
HincII GTYRAC 2 cut(s) 688, 796
HindII GTYRAC 2 cut(s) 688, 796
HinfI GANTC 3 cut(s) 71, 453, 925
HpaII CCGG 2 cut(s) 294, 897
HphI GGTGA 2 cut(s) 94, 167
Hpy166II GTNNAC 4 cut(s) 514, 688, 796, 1000
Hpy188I TCNGA 5 cut(s) 7, 169, 418, 620, 699
Hpy188III TCNNGA 4 cut(s) 78, 256, 268, 929
Hpy8I GTNNAC 4 cut(s) 514, 688, 796, 1000
Hpy99I CGWCG 1 cut(s) 392
HpyAV CCTTC 3 cut(s) 103, 182, 655
HpyCH4III ACNGT 5 cut(s) 152, 362, 634, 749, 763
HpyCH4IV ACGT 1 cut(s) 1056
HpyCH4V TGCA 9 cut(s) 26, 37, 197, 306, 339, 485, 724, 905, 947
HpyF10VI GCNNNNNNNGC 4 cut(s) 47, 336, 553, 902
HpyF3I CTNAG 3 cut(s) 166, 183, 852
HpySE526I ACGT 1 cut(s) 1056
Hsp92II CATG 6 cut(s) 139, 230, 310, 321, 551, 736
Ksp22I TGATCA 2 cut(s) 636, 699
Kzo9I GATC 3 cut(s) 392, 636, 699
LmnI GCTCC 4 cut(s) 258, 436, 775, 846
Lsp1109I GCAGC 3 cut(s) 49, 715, 959
MaeI CTAG 1 cut(s) 66
MaeII ACGT 1 cut(s) 1056
MaeIII GTNAC 3 cut(s) 244, 332, 628
MalI GATC 3 cut(s) 394, 638, 701
MboI GATC 3 cut(s) 392, 636, 699
MboII GAAGA 3 cut(s) 41, 151, 670
MfeI CAATTG 1 cut(s) 906
MhlI GDGCHC 1 cut(s) 838
MluCI AATT 7 cut(s) 12, 81, 272, 355, 422, 906, 1072
MlyI GAGTC 1 cut(s) 919
MmeI TCCRAC 3 cut(s) 262, 441, 868
MnlI CCTC 7 cut(s) 85, 102, 427, 469, 593, 709, 783
MroXI GAANNNNTTC 1 cut(s) 981
MseI TTAA 3 cut(s) 276, 803, 1035
MslI CAYNNNNRTG 4 cut(s) 509, 561, 986, 1055
MspA1I CMGCKG 2 cut(s) 862, 968
MspI CCGG 2 cut(s) 294, 897
MspR9I CCNGG 3 cut(s) 106, 222, 898
MunI CAATTG 1 cut(s) 906
Mva1269I GAATGC 1 cut(s) 52
MvaI CCWGG 2 cut(s) 106, 222
MwoI GCNNNNNNNGC 4 cut(s) 47, 336, 553, 902
NciI CCSGG 1 cut(s) 898
NcoI CCATGG 1 cut(s) 732
NdeI CATATG 1 cut(s) 591
NdeII GATC 3 cut(s) 392, 636, 699
NlaIII CATG 6 cut(s) 139, 230, 310, 321, 551, 736
NlaIV GGNNCC 2 cut(s) 581, 842
NmeAIII GCCGAG 1 cut(s) 817
NmuCI GTSAC 2 cut(s) 244, 628
OliI CACNNNNGTG 1 cut(s) 561
PcsI WCGNNNNNNNCGW 1 cut(s) 753
PctI GAATGC 1 cut(s) 52
PdmI GAANNNNTTC 1 cut(s) 981
PfeI GAWTC 2 cut(s) 71, 453
PkrI GCNGC 3 cut(s) 64, 730, 949
PleI GAGTC 1 cut(s) 919
PpsI GAGTC 1 cut(s) 919
PshAI GACNNNNGTC 1 cut(s) 251
PshBI ATTAAT 1 cut(s) 803
Psp6I CCWGG 2 cut(s) 104, 220
PspGI CCWGG 2 cut(s) 104, 220
PspN4I GGNNCC 2 cut(s) 581, 842
PspPI GGNCC 2 cut(s) 378, 580
PstI CTGCAG 2 cut(s) 28, 39
PvuII CAGCTG 1 cut(s) 968
RsaI GTAC 4 cut(s) 375, 450, 515, 643
RsaNI GTAC 4 cut(s) 374, 449, 514, 642
RseI CAYNNNNRTG 4 cut(s) 509, 561, 986, 1055
SaqAI TTAA 3 cut(s) 276, 803, 1035
SatI GCNGC 3 cut(s) 63, 729, 948
Sau3AI GATC 3 cut(s) 392, 636, 699
Sau96I GGNCC 2 cut(s) 378, 580
SchI GAGTC 1 cut(s) 919
ScrFI CCNGG 3 cut(s) 106, 222, 898
SduI GDGCHC 1 cut(s) 838
SfcI CTRYAG 2 cut(s) 24, 35
SinI GGWCC 1 cut(s) 378
SmiMI CAYNNNNRTG 4 cut(s) 509, 561, 986, 1055
SmlI CTYRAG 2 cut(s) 542, 927
SmoI CTYRAG 2 cut(s) 542, 927
Sse9I AATT 7 cut(s) 12, 81, 272, 355, 422, 906, 1072
SsiI CCGC 2 cut(s) 330, 860
SspMI CTAG 1 cut(s) 66
StyD4I CCNGG 3 cut(s) 104, 220, 896
StyI CCWWGG 2 cut(s) 609, 732
TaaI ACNGT 5 cut(s) 152, 362, 634, 749, 763
TaiI ACGT 1 cut(s) 1059
TaqI TCGA 3 cut(s) 395, 432, 756
TaqII GACCGA 2 cut(s) 263, 575
TasI AATT 7 cut(s) 12, 81, 272, 355, 422, 906, 1072
TatI WGTACW 2 cut(s) 513, 641
TfiI GAWTC 2 cut(s) 71, 453
Tru1I TTAA 3 cut(s) 276, 803, 1035
Tru9I TTAA 3 cut(s) 276, 803, 1035
TscAI CASTG 3 cut(s) 48, 603, 1009
TseFI GTSAC 2 cut(s) 244, 628
TseI GCWGC 3 cut(s) 62, 728, 947
Tsp45I GTSAC 2 cut(s) 244, 628
TspDTI ATGAA 4 cut(s) 152, 306, 729, 932
TspGWI ACGGA 4 cut(s) 69, 391, 405, 824
TspRI CASTG 3 cut(s) 48, 603, 1009
VpaK11BI GGWCC 1 cut(s) 378
VspI ATTAAT 1 cut(s) 803
XagI CCTNNNNNAGG 1 cut(s) 182
XapI RAATTY 4 cut(s) 81, 272, 422, 1072
XcmI CCANNNNNNNNNTGG 1 cut(s) 739
XmnI GAANNNNTTC 1 cut(s) 981
XspI CTAG 1 cut(s) 66
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.