Rh2CG223000

Dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0002881
Physical Location & Seq
Forward (+)
22406833 .. 22410670
3838 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG223000.1

Sequence Viewer

Length: 804 bp
ATGATGGGTTCATGTGGCTCATGCGAGAACTGCAAACAAGACTTGGAAAATTACTGCCCCAAAATGTTATGGACCTTCAGTGGACAATATGAAGATGGAACAAGAGCCTTTGGTGGATATTCCAATAAGTTTGTTGTGGAAGAGCACTTTGCCGTCCGAATCCCAAATGACATACCACTAGCAGGTGTCGCCCCGCTATTGTGTGCTGGGATTACCGTGTATAGCCCCCTGAAATATTTCGGACTAGTCCCCCAGCCCGGTAGCAAGAAACATTTGGGTGTTGTTGGGCTTGGAGGTGTAGGTCATATGGCCGTGAAGTTTGCCAAAGCTTTCGGAGCAAAGGTCACTGTCATTAGTTCCTCTCCGAGTAAGGAAAAGGAAGCAGTGGAACAACTTGGTGCAGATTCATTTTTGATCAGTCATGACCATGAGAAATTGGAGGCTGCCTTGGGCACCATGGATGGTATCATCGACACTGTTTCTGCTTCTCACTCTCTCCTCCCATTGATTGGCTTGCTAAAAACTAGTGGAAAACTAGTTTTGGTGGGTGCTCCAGTGGAGCCACCTGAGCTTCCCGTCTTCCCTTTAATTATGGGGAGGAAAGTTATTGCTGGTAGTGCAATTGGGGGGATGAAAGAGACTCAAGAGATGATAGATTTTGCAGCAAAGCACAACATCATAGCAGATGTCGAAGTTATTCCAATGGATTATGTGAACACTGCTCTTGAACGACTTGAGAAAGGCGACAATGTTAAATATCGCTTTGTCATCGATGTTGCCAACACAATAAAATGTGCAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
ADH_N ADH_zinc_N

Protein Analysis

267

Amino Acids

28.55

Weight (kDa)

5.99

Isoelectric Point (pI)

28.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh_C PF02826 88 - 132 3.7e-06 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
AlaDh_PNT_C PF01262 92 - 162 1.1e-06 Alanine dehydrogenase/PNT, C-terminal domain
ADH_zinc_N PF00107 99 - 222 4.1e-23 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 173
Acc36I ACCTGC 1 cut(s) 173
AccB1I GGYRCC 1 cut(s) 452
AccB7I CCANNNNNTGG 1 cut(s) 509
AciI CCGC 1 cut(s) 194
AcoI YGGCCR 1 cut(s) 309
AcuI CTGAAG 1 cut(s) 61
AfiI CCNNNNNNNGG 3 cut(s) 182, 257, 509
AgsI TTSAA 1 cut(s) 728
AhlI ACTAGT 3 cut(s) 244, 524, 535
AluBI AGCT 2 cut(s) 329, 571
AluI AGCT 2 cut(s) 329, 571
Alw21I GWGCWC 2 cut(s) 147, 553
Alw26I GTCTC 1 cut(s) 632
AoxI GGCC 1 cut(s) 309
ApeKI GCWGC 2 cut(s) 443, 662
Asp700I GAANNNNTTC 2 cut(s) 236, 696
AspS9I GGNCC 1 cut(s) 72
AsuC2I CCSGG 1 cut(s) 258
AvaII GGWCC 1 cut(s) 72
BaeGI GKGCMC 1 cut(s) 455
BanI GGYRCC 1 cut(s) 452
BbsI GAAGAC 1 cut(s) 571
Bbv12I GWGCWC 2 cut(s) 147, 553
BbvI GCAGC 2 cut(s) 430, 674
BccI CCATC 2 cut(s) 89, 455
BceAI ACGGC 2 cut(s) 137, 296
BclI TGATCA 1 cut(s) 414
BcnI CCSGG 1 cut(s) 258
BcoDI GTCTC 1 cut(s) 632
BcuI ACTAGT 3 cut(s) 244, 524, 535
BfaI CTAG 5 cut(s) 179, 245, 525, 536, 802
BfuAI ACCTGC 1 cut(s) 173
BisI GCNGC 2 cut(s) 444, 663
BlsI GCNGC 2 cut(s) 445, 664
Bme1390I CCNGG 1 cut(s) 258
Bme18I GGWCC 1 cut(s) 72
BmgT120I GGNCC 1 cut(s) 72
BmiI GGNNCC 2 cut(s) 454, 561
BmrFI CCNGG 1 cut(s) 258
BpiI GAAGAC 1 cut(s) 571
BpmI CTGGAG 1 cut(s) 537
Bpu10I CCTNAGC 1 cut(s) 567
BpuEI CTTGAG 2 cut(s) 627, 755
BpuMI CCSGG 1 cut(s) 258
Bsa29I ATCGAT 1 cut(s) 771
BsaJI CCNNGG 2 cut(s) 447, 456
BsaXI ACNNNNNCTCC 2 cut(s) 327, 357
Bsc4I CCNNNNNNNGG 3 cut(s) 182, 257, 509
Bse1I ACTGG 1 cut(s) 554
BseCI ATCGAT 1 cut(s) 771
BseDI CCNNGG 2 cut(s) 447, 456
BseGI GGATG 2 cut(s) 466, 636
BseLI CCNNNNNNNGG 3 cut(s) 182, 257, 509
BseMII CTCAG 1 cut(s) 558
BseNI ACTGG 1 cut(s) 554
BseRI GAGGAG 1 cut(s) 488
BseSI GKGCMC 1 cut(s) 455
BseXI GCAGC 2 cut(s) 430, 674
BseYI CCCAGC 2 cut(s) 206, 252
BsgI GTGCAG 1 cut(s) 420
BshFI GGCC 1 cut(s) 311
BshNI GGYRCC 1 cut(s) 452
BshVI ATCGAT 1 cut(s) 771
BsiHKAI GWGCWC 2 cut(s) 147, 553
BsiSI CCGG 1 cut(s) 258
BslFI GGGAC 1 cut(s) 233
BslI CCNNNNNNNGG 3 cut(s) 182, 257, 509
BsmAI GTCTC 1 cut(s) 632
BsmFI GGGAC 1 cut(s) 233
BsnI GGCC 1 cut(s) 311
Bsp1286I GDGCHC 3 cut(s) 147, 455, 553
Bsp143I GATC 1 cut(s) 414
Bsp19I CCATGG 1 cut(s) 456
BspACI CCGC 1 cut(s) 194
BspANI GGCC 1 cut(s) 311
BspCNI CTCAG 1 cut(s) 559
BspDI ATCGAT 1 cut(s) 771
BspHI TCATGA 1 cut(s) 421
BspLI GGNNCC 2 cut(s) 454, 561
BspMI ACCTGC 1 cut(s) 173
BspQI GCTCTTC 1 cut(s) 135
BspT107I GGYRCC 1 cut(s) 452
BsrI ACTGG 1 cut(s) 554
BssECI CCNNGG 2 cut(s) 447, 456
BssMI GATC 1 cut(s) 414
BssT1I CCWWGG 2 cut(s) 447, 456
Bst4CI ACNGT 3 cut(s) 217, 349, 478
Bst6I CTCTTC 1 cut(s) 135
BstC8I GCNNGC 1 cut(s) 515
BstDEI CTNAG 1 cut(s) 567
BstDSI CCRYGG 1 cut(s) 456
BstF5I GGATG 2 cut(s) 466, 636
BstKTI GATC 1 cut(s) 417
BstMAI GTCTC 1 cut(s) 632
BstMBI GATC 1 cut(s) 414
BstMWI GCNNNNNNNGC 5 cut(s) 30, 188, 335, 568, 617
BstSCI CCNGG 1 cut(s) 256
BstSLI GKGCMC 1 cut(s) 455
BstV1I GCAGC 2 cut(s) 430, 674
BstV2I GAAGAC 1 cut(s) 571
Bsu15I ATCGAT 1 cut(s) 771
BsuRI GGCC 1 cut(s) 311
BsuTUI ATCGAT 1 cut(s) 771
BtgI CCRYGG 1 cut(s) 456
BtsCI GGATG 2 cut(s) 466, 636
BtsI GCAGTG 2 cut(s) 390, 717
BtsIMutI CAGTG 6 cut(s) 85, 345, 390, 474, 561, 717
BveI ACCTGC 1 cut(s) 173
Cac8I GCNNGC 1 cut(s) 515
CciI TCATGA 1 cut(s) 421
Cfr13I GGNCC 1 cut(s) 72
ClaI ATCGAT 1 cut(s) 771
CviAII CATG 5 cut(s) 12, 21, 422, 428, 457
DdeI CTNAG 1 cut(s) 567
DpnI GATC 1 cut(s) 416
DpnII GATC 1 cut(s) 414
EaeI YGGCCR 1 cut(s) 309
Eam1104I CTCTTC 1 cut(s) 135
EarI CTCTTC 1 cut(s) 135
Eco130I CCWWGG 2 cut(s) 447, 456
Eco47I GGWCC 1 cut(s) 72
Eco57I CTGAAG 1 cut(s) 61
EcoT14I CCWWGG 2 cut(s) 447, 456
ErhI CCWWGG 2 cut(s) 447, 456
FaeI CATG 5 cut(s) 15, 24, 425, 431, 460
FaqI GGGAC 1 cut(s) 233
FatI CATG 5 cut(s) 11, 20, 421, 427, 456
FauI CCCGC 1 cut(s) 201
FauNDI CATATG 1 cut(s) 306
FbaI TGATCA 1 cut(s) 414
Fnu4HI GCNGC 2 cut(s) 444, 663
FokI GGATG 2 cut(s) 473, 643
Fsp4HI GCNGC 2 cut(s) 444, 663
FspBI CTAG 5 cut(s) 179, 245, 525, 536, 802
GluI GCNGC 2 cut(s) 444, 663
GsaI CCCAGC 2 cut(s) 210, 256
GsuI CTGGAG 1 cut(s) 537
HaeIII GGCC 1 cut(s) 311
HapII CCGG 1 cut(s) 258
Hin1II CATG 5 cut(s) 15, 24, 425, 431, 460
HindIII AAGCTT 1 cut(s) 327
HinfI GANTC 3 cut(s) 159, 404, 640
HpaII CCGG 1 cut(s) 258
Hpy166II GTNNAC 2 cut(s) 83, 715
Hpy188I TCNGA 4 cut(s) 158, 242, 335, 366
Hpy188III TCNNGA 3 cut(s) 422, 644, 725
Hpy8I GTNNAC 2 cut(s) 83, 715
HpyAV CCTTC 1 cut(s) 85
HpyCH4III ACNGT 3 cut(s) 217, 349, 478
HpyCH4V TGCA 5 cut(s) 33, 401, 620, 662, 797
HpyF10VI GCNNNNNNNGC 5 cut(s) 30, 188, 335, 568, 617
HpyF3I CTNAG 1 cut(s) 567
Hsp92II CATG 5 cut(s) 15, 24, 425, 431, 460
Ksp22I TGATCA 1 cut(s) 414
Kzo9I GATC 1 cut(s) 414
LguI GCTCTTC 1 cut(s) 135
LmnI GCTCC 3 cut(s) 335, 556, 559
LpnPI CCDG 8 cut(s) 168, 192, 242, 266, 271, 567, 579, 597
Lsp1109I GCAGC 2 cut(s) 430, 674
MaeI CTAG 5 cut(s) 179, 245, 525, 536, 802
MaeIII GTNAC 1 cut(s) 343
MalI GATC 1 cut(s) 416
MboI GATC 1 cut(s) 414
MboII GAAGA 3 cut(s) 104, 152, 571
MfeI CAATTG 1 cut(s) 621
MhlI GDGCHC 3 cut(s) 147, 455, 553
MluCI AATT 4 cut(s) 49, 434, 588, 621
MlyI GAGTC 1 cut(s) 634
MnlI CCTC 5 cut(s) 287, 370, 433, 509, 591
MroXI GAANNNNTTC 2 cut(s) 236, 696
MseI TTAA 2 cut(s) 587, 753
MslI CAYNNNNRTG 2 cut(s) 276, 426
MspI CCGG 1 cut(s) 258
MspR9I CCNGG 1 cut(s) 258
MunI CAATTG 1 cut(s) 621
MwoI GCNNNNNNNGC 5 cut(s) 30, 188, 335, 568, 617
NciI CCSGG 1 cut(s) 258
NcoI CCATGG 1 cut(s) 456
NdeI CATATG 1 cut(s) 306
NdeII GATC 1 cut(s) 414
NlaIII CATG 5 cut(s) 15, 24, 425, 431, 460
NlaIV GGNNCC 2 cut(s) 454, 561
NmuCI GTSAC 1 cut(s) 343
PagI TCATGA 1 cut(s) 421
PaqCI CACCTGC 1 cut(s) 173
PciSI GCTCTTC 1 cut(s) 135
PdmI GAANNNNTTC 2 cut(s) 236, 696
PfeI GAWTC 2 cut(s) 159, 404
PflMI CCANNNNNTGG 1 cut(s) 509
PkrI GCNGC 2 cut(s) 445, 664
PleI GAGTC 1 cut(s) 634
PpsI GAGTC 1 cut(s) 634
PspFI CCCAGC 2 cut(s) 206, 252
PspN4I GGNNCC 2 cut(s) 454, 561
PspPI GGNCC 1 cut(s) 72
RseI CAYNNNNRTG 2 cut(s) 276, 426
SapI GCTCTTC 1 cut(s) 135
SaqAI TTAA 2 cut(s) 587, 753
SatI GCNGC 2 cut(s) 444, 663
Sau3AI GATC 1 cut(s) 414
Sau96I GGNCC 1 cut(s) 72
SchI GAGTC 1 cut(s) 634
ScrFI CCNGG 1 cut(s) 258
SduI GDGCHC 3 cut(s) 147, 455, 553
SetI ASST 8 cut(s) 77, 187, 298, 304, 331, 345, 568, 573
SinI GGWCC 1 cut(s) 72
SmiMI CAYNNNNRTG 2 cut(s) 276, 426
SmlI CTYRAG 2 cut(s) 642, 734
SmoI CTYRAG 2 cut(s) 642, 734
SpeI ACTAGT 3 cut(s) 244, 524, 535
Sse9I AATT 4 cut(s) 49, 434, 588, 621
SsiI CCGC 1 cut(s) 194
SspI AATATT 1 cut(s) 236
SspMI CTAG 5 cut(s) 179, 245, 525, 536, 802
StyD4I CCNGG 1 cut(s) 256
StyI CCWWGG 2 cut(s) 447, 456
TaaI ACNGT 3 cut(s) 217, 349, 478
TaqI TCGA 3 cut(s) 471, 690, 771
TasI AATT 4 cut(s) 49, 434, 588, 621
TfiI GAWTC 2 cut(s) 159, 404
Tru1I TTAA 2 cut(s) 587, 753
Tru9I TTAA 2 cut(s) 587, 753
TscAI CASTG 6 cut(s) 85, 352, 390, 481, 561, 724
TseFI GTSAC 1 cut(s) 343
TseI GCWGC 2 cut(s) 443, 662
Tsp45I GTSAC 1 cut(s) 343
TspDTI ATGAA 3 cut(s) 105, 396, 647
TspRI CASTG 6 cut(s) 85, 352, 390, 481, 561, 724
Van91I CCANNNNNTGG 1 cut(s) 509
VpaK11BI GGWCC 1 cut(s) 72
XmnI GAANNNNTTC 2 cut(s) 236, 696
XspI CTAG 5 cut(s) 179, 245, 525, 536, 802
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.