Rh2BG230800

Dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0002881
Physical Location & Seq
Forward (+)
22721128 .. 22724910
3783 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG230800.1

Sequence Viewer

Length: 762 bp
ATGAGTGAAACAATTTTATCATCTGCAGAGGGAGTGCAGGCATATGCATGGGCTGCTAGAGATTCCTCAGGAATTCTCTCTCCCTTCCACTTCACCAGGAGGACTACTGGAAGTAATGATATCAGCATGAAGATACTCTACTGTGGTATTTGCCACTCAGACCTTGACCTTGCAAAGGACGAAGTTGGAATGACCATCTATCGGTATCCCATCGTCCCAGGACATGAGATCGTCGGAGAAGTGACCGAAGTCGGAAGCAACGTCACGAAATTCAAAGTAGGAGACATAGCCGGAGTGGGGTGCATGGTGGTTTCATGTGGTTCGTGCAATAACCGCACACGAGACTTGGAAAGTTACTGCCCCAATATGATCCATAACAAACACTACCACGACGGATCCAGAACTTTTGGGGGATACTCCGACAAACTAGTAGTCGAGGAGCATTTCGCAGTTCGAATTCCAGATAACTTGTCATTGGAAGGTGTTGCACCTTTGCTATGTACTGGGATTACGGTGTACAGCCCTACGAAACACTTTGGACTTTTTGAGCATGGAAGGAAGTTAATTGCTGGTAGTGCAATTGGGGGAATGAAAGAGACTCAAGAGATGGTAGATTTTGCAGCAAAGCATAACATCACAGCTGATGTTGAAGTCATTCCAATGGATTATGTGAACACTGCTCTTGAAAGAAAAATGATCACTGCAAGAGCTAAGCAGGAAAGCAGCCGAAATGGCTTTGTGGCACTAGCAGTGCAGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
ADH_N ADH_zinc_N

Protein Analysis

253

Amino Acids

27.63

Weight (kDa)

6.05

Isoelectric Point (pI)

38.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 38 - 155 2.9e-22 Alcohol dehydrogenase GroES-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 334
AclWI GGATC 3 cut(s) 364, 390, 403
AcsI RAATTY 3 cut(s) 72, 269, 456
AfaI GTAC 2 cut(s) 502, 518
AfiI CCNNNNNNNGG 3 cut(s) 175, 201, 297
AgsI TTSAA 3 cut(s) 274, 650, 686
AhlI ACTAGT 1 cut(s) 427
AjnI CCWGG 2 cut(s) 95, 217
AluBI AGCT 2 cut(s) 641, 710
AluI AGCT 2 cut(s) 641, 710
Alw26I GTCTC 3 cut(s) 276, 336, 590
AlwI GGATC 3 cut(s) 364, 390, 403
ApeKI GCWGC 4 cut(s) 53, 620, 723, 754
ApoI RAATTY 3 cut(s) 72, 269, 456
ArsI GACNNNNNNTTYG 2 cut(s) 417, 449
Asp700I GAANNNNTTC 1 cut(s) 654
AsuHPI GGTGA 1 cut(s) 85
AsuII TTCGAA 1 cut(s) 454
AxyI CCTNAGG 1 cut(s) 67
BamHI GGATCC 1 cut(s) 395
BarI GAAGNNNNNNTAC 2 cut(s) 122, 154
BauI CACGAG 1 cut(s) 339
BbvI GCAGC 3 cut(s) 40, 632, 735
BccI CCATC 3 cut(s) 203, 218, 601
BciT130I CCWGG 2 cut(s) 97, 219
BciVI GTATCC 2 cut(s) 216, 407
BclI TGATCA 1 cut(s) 696
BcoDI GTCTC 3 cut(s) 276, 336, 590
BcuI ACTAGT 1 cut(s) 427
BfaI CTAG 3 cut(s) 57, 428, 746
BfmI CTRYAG 1 cut(s) 24
BfuI GTATCC 2 cut(s) 216, 407
BglI GCCNNNNNGGC 1 cut(s) 732
BisI GCNGC 4 cut(s) 54, 621, 724, 755
BlpI GCTNAGC 1 cut(s) 711
BlsI GCNGC 4 cut(s) 55, 622, 725, 756
Bme1390I CCNGG 2 cut(s) 97, 219
BmiI GGNNCC 1 cut(s) 397
BmrFI CCNGG 2 cut(s) 97, 219
BmrI ACTGGG 1 cut(s) 513
BmuI ACTGGG 1 cut(s) 513
BoxI GACNNNNGTC 1 cut(s) 248
Bpu1102I GCTNAGC 1 cut(s) 711
Bpu14I TTCGAA 1 cut(s) 454
BpuEI CTTGAG 1 cut(s) 585
BsaJI CCNNGG 1 cut(s) 217
Bsc4I CCNNNNNNNGG 3 cut(s) 175, 201, 297
Bse1I ACTGG 2 cut(s) 112, 508
Bse21I CCTNAGG 1 cut(s) 67
BseBI CCWGG 2 cut(s) 97, 219
BseDI CCNNGG 1 cut(s) 217
BseLI CCNNNNNNNGG 3 cut(s) 175, 201, 297
BseMII CTCAG 2 cut(s) 81, 171
BseNI ACTGG 2 cut(s) 112, 508
BseRI GAGGAG 1 cut(s) 452
BseXI GCAGC 3 cut(s) 40, 632, 735
BsgI GTGCAG 1 cut(s) 56
BsiSI CCGG 1 cut(s) 291
BslFI GGGAC 1 cut(s) 200
BslI CCNNNNNNNGG 3 cut(s) 175, 201, 297
BsmAI GTCTC 3 cut(s) 276, 336, 590
BsmFI GGGAC 1 cut(s) 200
Bsp119I TTCGAA 1 cut(s) 454
Bsp1407I TGTACA 1 cut(s) 516
Bsp143I GATC 4 cut(s) 228, 369, 395, 696
Bsp1720I GCTNAGC 1 cut(s) 711
BspACI CCGC 1 cut(s) 334
BspCNI CTCAG 2 cut(s) 80, 170
BspLI GGNNCC 1 cut(s) 397
BspMAI CTGCAG 1 cut(s) 28
BspPI GGATC 3 cut(s) 364, 390, 403
BspT104I TTCGAA 1 cut(s) 454
BsrGI TGTACA 1 cut(s) 516
BsrI ACTGG 2 cut(s) 112, 508
BssECI CCNNGG 1 cut(s) 217
BssMI GATC 4 cut(s) 228, 369, 395, 696
BssSI CACGAG 1 cut(s) 339
Bst2BI CACGAG 1 cut(s) 339
Bst2UI CCWGG 2 cut(s) 97, 219
Bst4CI ACNGT 2 cut(s) 143, 514
BstAPI GCANNNNNTGC 1 cut(s) 53
BstAUI TGTACA 1 cut(s) 516
BstBI TTCGAA 1 cut(s) 454
BstC8I GCNNGC 1 cut(s) 39
BstDEI CTNAG 3 cut(s) 67, 157, 711
BstENI CCTNNNNNAGG 1 cut(s) 173
BstKTI GATC 4 cut(s) 231, 372, 398, 699
BstMAI GTCTC 3 cut(s) 276, 336, 590
BstMBI GATC 4 cut(s) 228, 369, 395, 696
BstMWI GCNNNNNNNGC 4 cut(s) 53, 333, 575, 732
BstNI CCWGG 2 cut(s) 97, 219
BstPAI GACNNNNGTC 1 cut(s) 248
BstSCI CCNGG 2 cut(s) 95, 217
BstSFI CTRYAG 1 cut(s) 24
BstV1I GCAGC 3 cut(s) 40, 632, 735
BstX2I RGATCY 1 cut(s) 395
BstYI RGATCY 1 cut(s) 395
Bsu36I CCTNAGG 1 cut(s) 67
BsuI GTATCC 2 cut(s) 216, 407
BtsI GCAGTG 3 cut(s) 675, 699, 756
BtsIMutI CAGTG 3 cut(s) 675, 699, 756
Cac8I GCNNGC 1 cut(s) 39
Csp6I GTAC 2 cut(s) 501, 517
CviAII CATG 6 cut(s) 48, 127, 224, 304, 315, 551
CviJI RGCY 7 cut(s) 53, 290, 522, 641, 710, 726, 735
CviKI_1 RGCY 7 cut(s) 53, 290, 522, 641, 710, 726, 735
CviQI GTAC 2 cut(s) 501, 517
DdeI CTNAG 3 cut(s) 67, 157, 711
DpnI GATC 4 cut(s) 230, 371, 397, 698
DpnII GATC 4 cut(s) 228, 369, 395, 696
Eco32I GATATC 1 cut(s) 121
Eco81I CCTNAGG 1 cut(s) 67
EcoNI CCTNNNNNAGG 1 cut(s) 173
EcoRI GAATTC 2 cut(s) 72, 456
EcoRII CCWGG 2 cut(s) 95, 217
EcoRV GATATC 1 cut(s) 121
EcoT22I ATGCAT 1 cut(s) 49
FaeI CATG 6 cut(s) 51, 130, 227, 307, 318, 554
FaqI GGGAC 1 cut(s) 200
FatI CATG 6 cut(s) 47, 126, 223, 303, 314, 550
FauNDI CATATG 1 cut(s) 43
FbaI TGATCA 1 cut(s) 696
Fnu4HI GCNGC 4 cut(s) 54, 621, 724, 755
Fsp4HI GCNGC 4 cut(s) 54, 621, 724, 755
FspBI CTAG 3 cut(s) 57, 428, 746
GluI GCNGC 4 cut(s) 54, 621, 724, 755
HapII CCGG 1 cut(s) 291
Hin1II CATG 6 cut(s) 51, 130, 227, 307, 318, 554
HinfI GANTC 2 cut(s) 62, 598
HpaII CCGG 1 cut(s) 291
HphI GGTGA 1 cut(s) 85
Hpy166II GTNNAC 2 cut(s) 517, 673
Hpy188I TCNGA 4 cut(s) 160, 236, 254, 421
Hpy188III TCNNGA 6 cut(s) 69, 265, 399, 461, 602, 683
Hpy8I GTNNAC 2 cut(s) 517, 673
Hpy99I CGWCG 2 cut(s) 236, 395
HpyAV CCTTC 3 cut(s) 94, 473, 549
HpyCH4III ACNGT 2 cut(s) 143, 514
HpyCH4IV ACGT 1 cut(s) 261
HpyF10VI GCNNNNNNNGC 4 cut(s) 53, 333, 575, 732
HpyF3I CTNAG 3 cut(s) 67, 157, 711
HpySE526I ACGT 1 cut(s) 261
Hsp92II CATG 6 cut(s) 51, 130, 227, 307, 318, 554
Ksp22I TGATCA 1 cut(s) 696
Kzo9I GATC 4 cut(s) 228, 369, 395, 696
LmnI GCTCC 1 cut(s) 439
Lsp1109I GCAGC 3 cut(s) 40, 632, 735
MaeI CTAG 3 cut(s) 57, 428, 746
MaeII ACGT 1 cut(s) 261
MaeIII GTNAC 3 cut(s) 241, 262, 353
MalI GATC 4 cut(s) 230, 371, 397, 698
MboI GATC 4 cut(s) 228, 369, 395, 696
MboII GAAGA 1 cut(s) 142
MfeI CAATTG 1 cut(s) 579
MflI RGATCY 1 cut(s) 395
MluCI AATT 6 cut(s) 12, 72, 269, 456, 564, 579
MlyI GAGTC 1 cut(s) 592
MmeI TCCRAC 4 cut(s) 166, 214, 232, 444
MnlI CCTC 4 cut(s) 22, 76, 93, 430
Mph1103I ATGCAT 1 cut(s) 49
MroXI GAANNNNTTC 1 cut(s) 654
MseI TTAA 1 cut(s) 563
MslI CAYNNNNRTG 2 cut(s) 46, 659
MspA1I CMGCKG 1 cut(s) 641
MspI CCGG 1 cut(s) 291
MspR9I CCNGG 2 cut(s) 97, 219
MunI CAATTG 1 cut(s) 579
MvaI CCWGG 2 cut(s) 97, 219
MwoI GCNNNNNNNGC 4 cut(s) 53, 333, 575, 732
NdeI CATATG 1 cut(s) 43
NdeII GATC 4 cut(s) 228, 369, 395, 696
NlaIII CATG 6 cut(s) 51, 130, 227, 307, 318, 554
NlaIV GGNNCC 1 cut(s) 397
NmuCI GTSAC 2 cut(s) 241, 262
NsiI ATGCAT 1 cut(s) 49
NspV TTCGAA 1 cut(s) 454
PcsI WCGNNNNNNNCGW 1 cut(s) 258
PdmI GAANNNNTTC 1 cut(s) 654
PfeI GAWTC 1 cut(s) 62
PkrI GCNGC 4 cut(s) 55, 622, 725, 756
PleI GAGTC 1 cut(s) 592
PpsI GAGTC 1 cut(s) 592
PshAI GACNNNNGTC 1 cut(s) 248
Psp6I CCWGG 2 cut(s) 95, 217
PspGI CCWGG 2 cut(s) 95, 217
PspN4I GGNNCC 1 cut(s) 397
PstI CTGCAG 1 cut(s) 28
PsuI RGATCY 1 cut(s) 395
PvuII CAGCTG 1 cut(s) 641
RsaI GTAC 2 cut(s) 502, 518
RsaNI GTAC 2 cut(s) 501, 517
RseI CAYNNNNRTG 2 cut(s) 46, 659
SaqAI TTAA 1 cut(s) 563
SatI GCNGC 4 cut(s) 54, 621, 724, 755
Sau3AI GATC 4 cut(s) 228, 369, 395, 696
SchI GAGTC 1 cut(s) 592
ScrFI CCNGG 2 cut(s) 97, 219
SetI ASST 7 cut(s) 165, 171, 264, 484, 493, 643, 712
SfcI CTRYAG 1 cut(s) 24
SfuI TTCGAA 1 cut(s) 454
SmiMI CAYNNNNRTG 2 cut(s) 46, 659
SmlI CTYRAG 1 cut(s) 600
SmoI CTYRAG 1 cut(s) 600
SpeI ACTAGT 1 cut(s) 427
Sse9I AATT 6 cut(s) 12, 72, 269, 456, 564, 579
SsiI CCGC 1 cut(s) 334
SspMI CTAG 3 cut(s) 57, 428, 746
StyD4I CCNGG 2 cut(s) 95, 217
TaaI ACNGT 2 cut(s) 143, 514
TaiI ACGT 1 cut(s) 264
TaqI TCGA 2 cut(s) 435, 454
TaqII GACCGA 1 cut(s) 260
TasI AATT 6 cut(s) 12, 72, 269, 456, 564, 579
TatI WGTACW 2 cut(s) 500, 516
TfiI GAWTC 1 cut(s) 62
Tru1I TTAA 1 cut(s) 563
Tru9I TTAA 1 cut(s) 563
TscAI CASTG 3 cut(s) 682, 706, 756
TseFI GTSAC 2 cut(s) 241, 262
TseI GCWGC 4 cut(s) 53, 620, 723, 754
Tsp45I GTSAC 2 cut(s) 241, 262
TspDTI ATGAA 3 cut(s) 143, 303, 605
TspGWI ACGGA 1 cut(s) 408
TspRI CASTG 3 cut(s) 682, 706, 756
XagI CCTNNNNNAGG 1 cut(s) 173
XapI RAATTY 3 cut(s) 72, 269, 456
XmnI GAANNNNTTC 1 cut(s) 654
XspI CTAG 3 cut(s) 57, 428, 746
Zsp2I ATGCAT 1 cut(s) 49
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.