Rroxscaffold_2G00133090

Dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002881
Physical Location & Seq
Reverse (-)
70212346 .. 70214294
1949 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00133090.1

Sequence Viewer

Length: 1299 bp
ATGCCTTCAGTCGCCGTTTTAAACTGGATTTGCCCGTTATCGCACATGGGTTGGATCCCAATTGTGCAACACAGGATTGTTCATCCTTCGGGTGGCTGTCCAGTTGATCCCTGTGTGGATCTGTTTACCTTTCGTCCTGCTTTGGCTTTTCTGGCTTCTACTTTGGCGATGGAAGATGTTCTCCGTTTCGGAGATGATCTGACATTTGTGGCTTCTTGTGGGTCTGGAATCGGGATATTCCGGTGCTCTTGTTTGAGTTCGTTGTTGGCTTTTTCCAAACTTTTCGACTCTGGCCTGATGGTGTTCTTCATACTTGGTGCGAATTTTATTCGCGGTGGCGCTACCGCTGTGGCAGCGTCCTTCTCTTGGCCAGATTGGGGTTTGGGTGGCCTAGACTCGCCTGGTTTGAGTCTGCATGAAATTGTTGGCCAAGTGACCAAGGTTGGGCTCAACGTCAAAAAATTCCAAGTTGGAGATGTAGCTGGAGTAGGGTGCATGATGGGCTCATGTGGCTCATGCGAGAACTGCAAACAAGACTTGGAAAATTACTGTCCCAAAATGTTATGGACCTTCAGTGGTCAATATGAAGATGGAACAAGAGCCTTTGGTGGATATTCAGATAAGTTTGTTGTGGAAGAGCACTTTGCCGTCCGAATCCCAAATGACATTCCACTAGCAGGTGCCGCCCCACTATTGTGTGCTGGGATTACCGTGTATAGCCCCCTGAAATATTTCGGACTAGTCCCCCAGCCCGGTAGCAAGAAACATTTGGGTGTTGTTGGGCTTGGAGGTGTAGGTCATATGGCCGTGAAGTTTGCCAAAGCTTTCGGAGCAAAGGTCACCGTGATTAGTTCCTCTCCGAGTAAGGAAAAGGAAGCAGTGGAACGACTTGGTGCAGATTCATTTTTGATTAGTCATGACCATGAGAAATTGGAGGCTGCCTTGGGCACCATGGATGGTATCATCGACACTGTTTCTGCTTCTCACTCTCTCCTCCCATTGATTGCCTTGCTGAAAACCGGTGGAAAACTAGTTTTGGTGGGTGCTCCAGTGGAGCCACCTGAGCTTCCCGTCTTCCCTTTAATTATGGGGAGGAAAGTTATTACTGGTAGTGCAATTGGGGGAATGAAAGAGACTCAAGAGATGATAGATTTTGCAGCAAAGCACAACATCACAGCAGATGTCGAAGTTATTCCAATGGATTATGTGAACACTGCTCTTGAAAGACTTGAGAAGGGTGACAATGTTAAATATCGCTTTGTCATCGATGTTGCCAACACGATAAAATGTGCAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
ADH_N ADH_zinc_N

Protein Analysis

432

Amino Acids

45.93

Weight (kDa)

6.0

Isoelectric Point (pI)

27.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 134 - 220 7.3e-13 Alcohol dehydrogenase GroES-like domain
2-Hacid_dh_C PF02826 253 - 296 9.3e-06 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
AlaDh_PNT_C PF01262 257 - 327 3.7e-06 Alanine dehydrogenase/PNT, C-terminal domain
ADH_zinc_N PF00107 264 - 387 9.7e-24 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 668
Acc36I ACCTGC 1 cut(s) 668
AccB1I GGYRCC 2 cut(s) 680, 947
AccII CGCG 1 cut(s) 333
AciI CCGC 3 cut(s) 333, 345, 684
AclWI GGATC 4 cut(s) 49, 62, 101, 126
AcoI YGGCCR 3 cut(s) 368, 427, 804
AcsI RAATTY 2 cut(s) 322, 461
AcuI CTGAAG 1 cut(s) 556
AfiI CCNNNNNNNGG 6 cut(s) 92, 366, 377, 444, 677, 752
AgeI ACCGGT 1 cut(s) 1019
AgsI TTSAA 1 cut(s) 1223
AhlI ACTAGT 2 cut(s) 739, 1030
AjnI CCWGG 1 cut(s) 400
AleI CACNNNNGTG 1 cut(s) 694
AluBI AGCT 3 cut(s) 482, 824, 1066
AluI AGCT 3 cut(s) 482, 824, 1066
Alw21I GWGCWC 3 cut(s) 248, 642, 1048
Alw26I GTCTC 1 cut(s) 1127
AlwI GGATC 4 cut(s) 49, 62, 101, 126
AoxI GGCC 5 cut(s) 292, 368, 388, 427, 804
ApeKI GCWGC 3 cut(s) 353, 938, 1157
ApoI RAATTY 2 cut(s) 322, 461
AsiGI ACCGGT 1 cut(s) 1019
Asp700I GAANNNNTTC 3 cut(s) 177, 731, 1191
AspLEI GCGC 1 cut(s) 341
AspS9I GGNCC 1 cut(s) 567
AsuC2I CCSGG 1 cut(s) 753
AsuHPI GGTGA 2 cut(s) 832, 1250
AvaII GGWCC 1 cut(s) 567
BaeGI GKGCMC 1 cut(s) 950
BalI TGGCCA 2 cut(s) 370, 429
BamHI GGATCC 1 cut(s) 54
BanI GGYRCC 2 cut(s) 680, 947
BanII GRGCYC 2 cut(s) 450, 506
BbsI GAAGAC 1 cut(s) 1066
Bbv12I GWGCWC 3 cut(s) 248, 642, 1048
BbvI GCAGC 3 cut(s) 365, 925, 1169
BccI CCATC 5 cut(s) 163, 292, 493, 584, 950
BceAI ACGGC 2 cut(s) 632, 791
BciT130I CCWGG 1 cut(s) 402
BcnI CCSGG 1 cut(s) 753
BcoDI GTCTC 1 cut(s) 1127
BcuI ACTAGT 2 cut(s) 739, 1030
BfaI CTAG 5 cut(s) 392, 674, 740, 1031, 1297
BfoI RGCGCY 1 cut(s) 342
BfuAI ACCTGC 1 cut(s) 668
BisI GCNGC 4 cut(s) 354, 684, 939, 1158
BlsI GCNGC 4 cut(s) 355, 685, 940, 1159
Bme1390I CCNGG 2 cut(s) 402, 753
Bme18I GGWCC 1 cut(s) 567
BmgT120I GGNCC 1 cut(s) 567
BmiI GGNNCC 4 cut(s) 56, 682, 949, 1056
BmrFI CCNGG 2 cut(s) 402, 753
BpiI GAAGAC 1 cut(s) 1066
BpmI CTGGAG 2 cut(s) 504, 1032
Bpu10I CCTNAGC 1 cut(s) 1062
BpuEI CTTGAG 2 cut(s) 1122, 1250
BpuMI CCSGG 1 cut(s) 753
Bsa29I ATCGAT 1 cut(s) 1266
BsaJI CCNNGG 3 cut(s) 438, 942, 951
BsaWI WCCGGW 2 cut(s) 240, 1019
BsaXI ACNNNNNCTCC 2 cut(s) 822, 852
Bsc4I CCNNNNNNNGG 6 cut(s) 92, 366, 377, 444, 677, 752
Bse118I RCCGGY 1 cut(s) 1019
Bse1I ACTGG 4 cut(s) 29, 101, 1049, 1111
BseBI CCWGG 1 cut(s) 402
BseCI ATCGAT 1 cut(s) 1266
BseDI CCNNGG 3 cut(s) 438, 942, 951
BseGI GGATG 2 cut(s) 82, 961
BseLI CCNNNNNNNGG 6 cut(s) 92, 366, 377, 444, 677, 752
BseMII CTCAG 1 cut(s) 1053
BseNI ACTGG 4 cut(s) 29, 101, 1049, 1111
BseRI GAGGAG 1 cut(s) 983
BseSI GKGCMC 1 cut(s) 950
BseXI GCAGC 3 cut(s) 365, 925, 1169
BseYI CCCAGC 2 cut(s) 701, 747
BsgI GTGCAG 1 cut(s) 915
Bsh1236I CGCG 1 cut(s) 333
BshFI GGCC 5 cut(s) 294, 370, 390, 429, 806
BshNI GGYRCC 2 cut(s) 680, 947
BshTI ACCGGT 1 cut(s) 1019
BshVI ATCGAT 1 cut(s) 1266
BsiHKAI GWGCWC 3 cut(s) 248, 642, 1048
BsiSI CCGG 3 cut(s) 241, 753, 1020
BslFI GGGAC 2 cut(s) 537, 728
BslI CCNNNNNNNGG 6 cut(s) 92, 366, 377, 444, 677, 752
BsmAI GTCTC 1 cut(s) 1127
BsmFI GGGAC 2 cut(s) 537, 728
BsnI GGCC 5 cut(s) 294, 370, 390, 429, 806
Bsp1286I GDGCHC 6 cut(s) 248, 450, 506, 642, 950, 1048
Bsp143I GATC 4 cut(s) 54, 106, 118, 196
Bsp19I CCATGG 1 cut(s) 951
BspACI CCGC 3 cut(s) 333, 345, 684
BspANI GGCC 5 cut(s) 294, 370, 390, 429, 806
BspCNI CTCAG 1 cut(s) 1054
BspDI ATCGAT 1 cut(s) 1266
BspFNI CGCG 1 cut(s) 333
BspHI TCATGA 1 cut(s) 916
BspLI GGNNCC 4 cut(s) 56, 682, 949, 1056
BspMI ACCTGC 1 cut(s) 668
BspPI GGATC 4 cut(s) 49, 62, 101, 126
BspQI GCTCTTC 1 cut(s) 630
BspT107I GGYRCC 2 cut(s) 680, 947
BsrFI RCCGGY 1 cut(s) 1019
BsrI ACTGG 4 cut(s) 29, 101, 1049, 1111
BssAI RCCGGY 1 cut(s) 1019
BssECI CCNNGG 3 cut(s) 438, 942, 951
BssMI GATC 4 cut(s) 54, 106, 118, 196
BssT1I CCWWGG 3 cut(s) 438, 942, 951
Bst2UI CCWGG 1 cut(s) 402
Bst4CI ACNGT 4 cut(s) 551, 712, 844, 973
Bst6I CTCTTC 1 cut(s) 630
BstDEI CTNAG 1 cut(s) 1062
BstDSI CCRYGG 1 cut(s) 951
BstEII GGTNACC 1 cut(s) 838
BstF5I GGATG 2 cut(s) 82, 961
BstFNI CGCG 1 cut(s) 333
BstH2I RGCGCY 1 cut(s) 342
BstHHI GCGC 1 cut(s) 341
BstKTI GATC 4 cut(s) 57, 109, 121, 199
BstMAI GTCTC 1 cut(s) 1127
BstMBI GATC 4 cut(s) 54, 106, 118, 196
BstMWI GCNNNNNNNGC 8 cut(s) 152, 353, 501, 510, 525, 683, 830, 1063
BstNI CCWGG 1 cut(s) 402
BstPI GGTNACC 1 cut(s) 838
BstSCI CCNGG 2 cut(s) 400, 751
BstSLI GKGCMC 1 cut(s) 950
BstUI CGCG 1 cut(s) 333
BstV1I GCAGC 3 cut(s) 365, 925, 1169
BstV2I GAAGAC 1 cut(s) 1066
BstX2I RGATCY 2 cut(s) 54, 118
BstYI RGATCY 2 cut(s) 54, 118
Bsu15I ATCGAT 1 cut(s) 1266
BsuRI GGCC 5 cut(s) 294, 370, 390, 429, 806
BsuTUI ATCGAT 1 cut(s) 1266
BtgI CCRYGG 1 cut(s) 951
BtgZI GCGATG 1 cut(s) 182
BtsCI GGATG 2 cut(s) 82, 961
BtsI GCAGTG 2 cut(s) 885, 1212
BtsIMutI CAGTG 5 cut(s) 580, 885, 969, 1056, 1212
BveI ACCTGC 1 cut(s) 668
CciI TCATGA 1 cut(s) 916
CfoI GCGC 1 cut(s) 341
Cfr10I RCCGGY 1 cut(s) 1019
Cfr13I GGNCC 1 cut(s) 567
ClaI ATCGAT 1 cut(s) 1266
CseI GACGC 1 cut(s) 345
CspAI ACCGGT 1 cut(s) 1019
CviAII CATG 8 cut(s) 46, 416, 496, 507, 516, 917, 923, 952
DdeI CTNAG 1 cut(s) 1062
DpnI GATC 4 cut(s) 56, 108, 120, 198
DpnII GATC 4 cut(s) 54, 106, 118, 196
DraI TTTAAA 1 cut(s) 21
EaeI YGGCCR 3 cut(s) 368, 427, 804
Eam1104I CTCTTC 1 cut(s) 630
EarI CTCTTC 1 cut(s) 630
Eco130I CCWWGG 3 cut(s) 438, 942, 951
Eco24I GRGCYC 2 cut(s) 450, 506
Eco47I GGWCC 1 cut(s) 567
Eco57I CTGAAG 1 cut(s) 556
Eco91I GGTNACC 1 cut(s) 838
EcoO65I GGTNACC 1 cut(s) 838
EcoRII CCWGG 1 cut(s) 400
EcoT14I CCWWGG 3 cut(s) 438, 942, 951
EcoT38I GRGCYC 2 cut(s) 450, 506
ErhI CCWWGG 3 cut(s) 438, 942, 951
FaeI CATG 8 cut(s) 49, 419, 499, 510, 519, 920, 926, 955
FaqI GGGAC 2 cut(s) 537, 728
FatI CATG 8 cut(s) 45, 415, 495, 506, 515, 916, 922, 951
FauNDI CATATG 1 cut(s) 801
Fnu4HI GCNGC 4 cut(s) 354, 684, 939, 1158
FokI GGATG 2 cut(s) 69, 968
FriOI GRGCYC 2 cut(s) 450, 506
Fsp4HI GCNGC 4 cut(s) 354, 684, 939, 1158
FspBI CTAG 5 cut(s) 392, 674, 740, 1031, 1297
GlaI GCGC 1 cut(s) 340
GluI GCNGC 4 cut(s) 354, 684, 939, 1158
GsaI CCCAGC 2 cut(s) 705, 751
GsuI CTGGAG 2 cut(s) 504, 1032
HaeII RGCGCY 1 cut(s) 342
HaeIII GGCC 5 cut(s) 294, 370, 390, 429, 806
HapII CCGG 3 cut(s) 241, 753, 1020
HgaI GACGC 1 cut(s) 345
HhaI GCGC 1 cut(s) 341
Hin1II CATG 8 cut(s) 49, 419, 499, 510, 519, 920, 926, 955
Hin6I GCGC 1 cut(s) 339
HinP1I GCGC 1 cut(s) 339
HindIII AAGCTT 1 cut(s) 822
HinfI GANTC 7 cut(s) 228, 287, 395, 409, 654, 899, 1135
HpaII CCGG 3 cut(s) 241, 753, 1020
HphI GGTGA 2 cut(s) 832, 1250
Hpy166II GTNNAC 2 cut(s) 126, 1210
Hpy188I TCNGA 7 cut(s) 191, 201, 619, 653, 737, 830, 861
Hpy188III TCNNGA 5 cut(s) 225, 232, 917, 1139, 1220
Hpy8I GTNNAC 2 cut(s) 126, 1210
HpyAV CCTTC 5 cut(s) 15, 96, 370, 580, 1228
HpyCH4III ACNGT 4 cut(s) 551, 712, 844, 973
HpyCH4IV ACGT 1 cut(s) 453
HpyCH4V TGCA 8 cut(s) 67, 415, 495, 528, 896, 1115, 1157, 1292
HpyF10VI GCNNNNNNNGC 8 cut(s) 152, 353, 501, 510, 525, 683, 830, 1063
HpyF3I CTNAG 1 cut(s) 1062
HpySE526I ACGT 1 cut(s) 453
Hsp92II CATG 8 cut(s) 49, 419, 499, 510, 519, 920, 926, 955
HspAI GCGC 1 cut(s) 339
Kzo9I GATC 4 cut(s) 54, 106, 118, 196
LguI GCTCTTC 1 cut(s) 630
LmnI GCTCC 3 cut(s) 830, 1051, 1054
Lsp1109I GCAGC 3 cut(s) 365, 925, 1169
MaeI CTAG 5 cut(s) 392, 674, 740, 1031, 1297
MaeII ACGT 1 cut(s) 453
MaeIII GTNAC 3 cut(s) 433, 838, 1238
MalI GATC 4 cut(s) 56, 108, 120, 198
MboI GATC 4 cut(s) 54, 106, 118, 196
MboII GAAGA 5 cut(s) 185, 298, 599, 647, 1066
MfeI CAATTG 2 cut(s) 60, 1116
MflI RGATCY 2 cut(s) 54, 118
MhlI GDGCHC 6 cut(s) 248, 450, 506, 642, 950, 1048
MlsI TGGCCA 2 cut(s) 370, 429
MluCI AATT 8 cut(s) 60, 322, 420, 461, 544, 929, 1083, 1116
MluNI TGGCCA 2 cut(s) 370, 429
MlyI GAGTC 4 cut(s) 281, 389, 418, 1129
MmeI TCCRAC 2 cut(s) 32, 451
MnlI CCTC 5 cut(s) 782, 865, 928, 1004, 1086
Mox20I TGGCCA 2 cut(s) 370, 429
MroXI GAANNNNTTC 3 cut(s) 177, 731, 1191
MscI TGGCCA 2 cut(s) 370, 429
MseI TTAA 3 cut(s) 20, 1082, 1248
MslI CAYNNNNRTG 3 cut(s) 694, 771, 921
Msp20I TGGCCA 2 cut(s) 370, 429
MspA1I CMGCKG 1 cut(s) 347
MspI CCGG 3 cut(s) 241, 753, 1020
MspR9I CCNGG 2 cut(s) 402, 753
MunI CAATTG 2 cut(s) 60, 1116
MvaI CCWGG 1 cut(s) 402
MvnI CGCG 1 cut(s) 333
MwoI GCNNNNNNNGC 8 cut(s) 152, 353, 501, 510, 525, 683, 830, 1063
NciI CCSGG 1 cut(s) 753
NcoI CCATGG 1 cut(s) 951
NdeI CATATG 1 cut(s) 801
NdeII GATC 4 cut(s) 54, 106, 118, 196
NlaIII CATG 8 cut(s) 49, 419, 499, 510, 519, 920, 926, 955
NlaIV GGNNCC 4 cut(s) 56, 682, 949, 1056
NmuCI GTSAC 3 cut(s) 433, 838, 1238
OliI CACNNNNGTG 1 cut(s) 694
PagI TCATGA 1 cut(s) 916
PaqCI CACCTGC 1 cut(s) 668
PciSI GCTCTTC 1 cut(s) 630
PdmI GAANNNNTTC 3 cut(s) 177, 731, 1191
PfeI GAWTC 3 cut(s) 228, 654, 899
PinAI ACCGGT 1 cut(s) 1019
PkrI GCNGC 4 cut(s) 355, 685, 940, 1159
PleI GAGTC 4 cut(s) 281, 389, 417, 1129
PpsI GAGTC 4 cut(s) 281, 389, 417, 1129
Psp6I CCWGG 1 cut(s) 400
PspEI GGTNACC 1 cut(s) 838
PspFI CCCAGC 2 cut(s) 701, 747
PspGI CCWGG 1 cut(s) 400
PspN4I GGNNCC 4 cut(s) 56, 682, 949, 1056
PspPI GGNCC 1 cut(s) 567
PsuI RGATCY 2 cut(s) 54, 118
RseI CAYNNNNRTG 3 cut(s) 694, 771, 921
SapI GCTCTTC 1 cut(s) 630
SaqAI TTAA 3 cut(s) 20, 1082, 1248
SatI GCNGC 4 cut(s) 354, 684, 939, 1158
Sau3AI GATC 4 cut(s) 54, 106, 118, 196
Sau96I GGNCC 1 cut(s) 567
SchI GAGTC 4 cut(s) 281, 389, 418, 1129
ScrFI CCNGG 2 cut(s) 402, 753
SduI GDGCHC 6 cut(s) 248, 450, 506, 642, 950, 1048
SinI GGWCC 1 cut(s) 567
SmiMI CAYNNNNRTG 3 cut(s) 694, 771, 921
SmlI CTYRAG 2 cut(s) 1137, 1229
SmoI CTYRAG 2 cut(s) 1137, 1229
SpeI ACTAGT 2 cut(s) 739, 1030
Sse9I AATT 8 cut(s) 60, 322, 420, 461, 544, 929, 1083, 1116
SsiI CCGC 3 cut(s) 333, 345, 684
SspI AATATT 1 cut(s) 731
SspMI CTAG 5 cut(s) 392, 674, 740, 1031, 1297
StyD4I CCNGG 2 cut(s) 400, 751
StyI CCWWGG 3 cut(s) 438, 942, 951
TaaI ACNGT 4 cut(s) 551, 712, 844, 973
TaiI ACGT 1 cut(s) 456
TaqI TCGA 4 cut(s) 285, 966, 1185, 1266
TasI AATT 8 cut(s) 60, 322, 420, 461, 544, 929, 1083, 1116
TauI GCSGC 1 cut(s) 686
TfiI GAWTC 3 cut(s) 228, 654, 899
Tru1I TTAA 3 cut(s) 20, 1082, 1248
Tru9I TTAA 3 cut(s) 20, 1082, 1248
TscAI CASTG 5 cut(s) 580, 885, 976, 1056, 1219
TseFI GTSAC 3 cut(s) 433, 838, 1238
TseI GCWGC 3 cut(s) 353, 938, 1157
Tsp45I GTSAC 3 cut(s) 433, 838, 1238
TspDTI ATGAA 6 cut(s) 71, 298, 432, 600, 891, 1142
TspGWI ACGGA 1 cut(s) 173
TspRI CASTG 5 cut(s) 580, 885, 976, 1056, 1219
VpaK11BI GGWCC 1 cut(s) 567
XapI RAATTY 2 cut(s) 322, 461
XmnI GAANNNNTTC 3 cut(s) 177, 731, 1191
XspI CTAG 5 cut(s) 392, 674, 740, 1031, 1297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.