MD00G1085800.v1.1

Dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0002881
Physical Location & Seq
Forward (+)
17338217 .. 17339201
985 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1085800.v1.1.491

Sequence Viewer

Length: 561 bp
ATGTCTCAAGAAATATCAGTTGCAGAAGAGGAAGTAGTGAAAGCCTATGGATGGGCCGCTAGAGATTCCTCGGGGGTTCTCTCTCCATTCCACTTCACTAGGAGAGCCACTGGTAATAATGATATCCGCATCAAGATACTCTATTGTGGCATTTGCCACTCAGACCTTCACCTTGCTAAGGACGAAGTTGGAATGACAATCTATCCCATCGTCCCAGGTCATGAGATTGTAGGCGAAGTGACAAAGGTTGGGTGCAATGTCACAAAATTCAAAGTAGGAGACATAGCTGGAGTTGGGTGCATGGTGGGTTCATGTCGCTCATGCAATAACTGCACGCAGGACTTAGAAAATTACTGCCCCAAAATGGTGTGGACATACAACAAACACCACGAAGACGGATCAAGAACATTTGGTGGATATTCGGACAAACTAGTAGTCGATGAACACTTTGCTGTCCAAATCCCAGATAACTTGTTGTTACAAGGTACTGCACCTATGCTGTGTGCTGGGATTCACAGTGTACAGTCCAATGAGATACTTTGGACTTATGGAGCCTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
ADH_N ADH_zinc_N

Protein Analysis

187

Amino Acids

20.49

Weight (kDa)

5.7

Isoelectric Point (pI)

41.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 39 - 156 3e-24 Alcohol dehydrogenase GroES-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 57, 127
AclWI GGATC 1 cut(s) 406
AcsI RAATTY 1 cut(s) 266
AfaI GTAC 2 cut(s) 487, 522
AfiI CCNNNNNNNGG 3 cut(s) 51, 178, 364
AgsI TTSAA 1 cut(s) 271
AhlI ACTAGT 1 cut(s) 430
AjnI CCWGG 2 cut(s) 214, 554
AluBI AGCT 1 cut(s) 287
AluI AGCT 1 cut(s) 287
Alw26I GTCTC 2 cut(s) 9, 273
AlwI GGATC 1 cut(s) 406
Ama87I CYCGRG 1 cut(s) 70
AoxI GGCC 1 cut(s) 54
ApoI RAATTY 1 cut(s) 266
ArsI GACNNNNNNTTYG 2 cut(s) 420, 452
AspS9I GGNCC 1 cut(s) 54
AsuHPI GGTGA 1 cut(s) 161
AvaI CYCGRG 1 cut(s) 70
BbsI GAAGAC 1 cut(s) 399
BccI CCATC 2 cut(s) 45, 215
BciT130I CCWGG 2 cut(s) 216, 556
BcoDI GTCTC 2 cut(s) 9, 273
BcuI ACTAGT 1 cut(s) 430
BfaI CTAG 3 cut(s) 60, 99, 431
BisI GCNGC 1 cut(s) 57
BlsI GCNGC 1 cut(s) 58
Bme1390I CCNGG 2 cut(s) 216, 556
BmeT110I CYCGRG 1 cut(s) 70
BmgT120I GGNCC 1 cut(s) 54
BmiI GGNNCC 1 cut(s) 553
BmrFI CCNGG 2 cut(s) 216, 556
BmsI GCATC 1 cut(s) 138
BpiI GAAGAC 1 cut(s) 399
BpmI CTGGAG 1 cut(s) 309
Bpu10I CCTNAGC 1 cut(s) 177
BsaJI CCNNGG 2 cut(s) 69, 214
Bsc4I CCNNNNNNNGG 3 cut(s) 51, 178, 364
Bse1I ACTGG 1 cut(s) 115
Bse3DI GCAATG 1 cut(s) 262
BseBI CCWGG 2 cut(s) 216, 556
BseDI CCNNGG 2 cut(s) 69, 214
BseGI GGATG 1 cut(s) 56
BseLI CCNNNNNNNGG 3 cut(s) 51, 178, 364
BseMI GCAATG 1 cut(s) 262
BseMII CTCAG 1 cut(s) 174
BseNI ACTGG 1 cut(s) 115
BseYI CCCAGC 1 cut(s) 506
BsgI GTGCAG 2 cut(s) 316, 474
BshFI GGCC 1 cut(s) 56
BsiHKCI CYCGRG 1 cut(s) 70
BslFI GGGAC 1 cut(s) 197
BslI CCNNNNNNNGG 3 cut(s) 51, 178, 364
BsmAI GTCTC 2 cut(s) 9, 273
BsmFI GGGAC 1 cut(s) 197
BsnI GGCC 1 cut(s) 56
BsoBI CYCGRG 1 cut(s) 70
Bsp1407I TGTACA 1 cut(s) 520
Bsp143I GATC 1 cut(s) 398
BspACI CCGC 2 cut(s) 57, 127
BspANI GGCC 1 cut(s) 56
BspCNI CTCAG 1 cut(s) 173
BspHI TCATGA 1 cut(s) 220
BspLI GGNNCC 1 cut(s) 553
BspPI GGATC 1 cut(s) 406
BsrDI GCAATG 1 cut(s) 262
BsrGI TGTACA 1 cut(s) 520
BsrI ACTGG 1 cut(s) 115
BssECI CCNNGG 2 cut(s) 69, 214
BssMI GATC 1 cut(s) 398
Bst2UI CCWGG 2 cut(s) 216, 556
Bst4CI ACNGT 2 cut(s) 518, 525
Bst6I CTCTTC 1 cut(s) 21
BstAPI GCANNNNNTGC 1 cut(s) 330
BstAUI TGTACA 1 cut(s) 520
BstC8I GCNNGC 1 cut(s) 335
BstDEI CTNAG 3 cut(s) 160, 177, 343
BstENI CCTNNNNNAGG 1 cut(s) 176
BstF5I GGATG 1 cut(s) 56
BstKTI GATC 1 cut(s) 401
BstMAI GTCTC 2 cut(s) 9, 273
BstMBI GATC 1 cut(s) 398
BstMWI GCNNNNNNNGC 1 cut(s) 330
BstNI CCWGG 2 cut(s) 216, 556
BstSCI CCNGG 2 cut(s) 214, 554
BstV2I GAAGAC 1 cut(s) 399
BsuRI GGCC 1 cut(s) 56
BtsCI GGATG 1 cut(s) 56
BtsIMutI CAGTG 2 cut(s) 108, 523
Cac8I GCNNGC 1 cut(s) 335
CciI TCATGA 1 cut(s) 220
Cfr13I GGNCC 1 cut(s) 54
Csp6I GTAC 2 cut(s) 486, 521
CviAII CATG 4 cut(s) 221, 301, 312, 321
CviJI RGCY 5 cut(s) 44, 56, 107, 287, 554
CviKI_1 RGCY 5 cut(s) 44, 56, 107, 287, 554
CviQI GTAC 2 cut(s) 486, 521
DdeI CTNAG 3 cut(s) 160, 177, 343
DpnI GATC 1 cut(s) 400
DpnII GATC 1 cut(s) 398
Eam1104I CTCTTC 1 cut(s) 21
EarI CTCTTC 1 cut(s) 21
Eco32I GATATC 1 cut(s) 124
Eco88I CYCGRG 1 cut(s) 70
EcoNI CCTNNNNNAGG 1 cut(s) 176
EcoRII CCWGG 2 cut(s) 214, 554
EcoRV GATATC 1 cut(s) 124
FaeI CATG 4 cut(s) 224, 304, 315, 324
FaiI YATR 9 cut(s) 48, 222, 284, 302, 313, 322, 376, 497, 549
FaqI GGGAC 1 cut(s) 197
FatI CATG 4 cut(s) 220, 300, 311, 320
Fnu4HI GCNGC 1 cut(s) 57
FokI GGATG 1 cut(s) 63
Fsp4HI GCNGC 1 cut(s) 57
FspBI CTAG 3 cut(s) 60, 99, 431
GluI GCNGC 1 cut(s) 57
GsaI CCCAGC 1 cut(s) 510
GsuI CTGGAG 1 cut(s) 309
HaeIII GGCC 1 cut(s) 56
Hin1II CATG 4 cut(s) 224, 304, 315, 324
HinfI GANTC 2 cut(s) 65, 511
HphI GGTGA 1 cut(s) 161
Hpy166II GTNNAC 2 cut(s) 372, 521
Hpy188I TCNGA 2 cut(s) 163, 424
Hpy188III TCNNGA 4 cut(s) 8, 133, 221, 402
Hpy8I GTNNAC 2 cut(s) 372, 521
HpyAV CCTTC 1 cut(s) 176
HpyCH4III ACNGT 2 cut(s) 518, 525
HpyCH4V TGCA 6 cut(s) 23, 255, 300, 324, 333, 491
HpyF10VI GCNNNNNNNGC 1 cut(s) 330
HpyF3I CTNAG 3 cut(s) 160, 177, 343
Hsp92II CATG 4 cut(s) 224, 304, 315, 324
Kzo9I GATC 1 cut(s) 398
LmnI GCTCC 1 cut(s) 551
LpnPI CCDG 8 cut(s) 96, 201, 228, 273, 323, 477, 492, 541
LweI GCATC 1 cut(s) 138
MaeI CTAG 3 cut(s) 60, 99, 431
MaeIII GTNAC 3 cut(s) 238, 259, 477
MalI GATC 1 cut(s) 400
MboI GATC 1 cut(s) 398
MboII GAAGA 2 cut(s) 38, 404
MluCI AATT 2 cut(s) 266, 349
MmeI TCCRAC 1 cut(s) 169
MnlI CCTC 2 cut(s) 22, 79
MspR9I CCNGG 2 cut(s) 216, 556
MvaI CCWGG 2 cut(s) 216, 556
MwoI GCNNNNNNNGC 1 cut(s) 330
NdeII GATC 1 cut(s) 398
NlaIII CATG 4 cut(s) 224, 304, 315, 324
NlaIV GGNNCC 1 cut(s) 553
NmuCI GTSAC 2 cut(s) 238, 259
PagI TCATGA 1 cut(s) 220
PfeI GAWTC 2 cut(s) 65, 511
PkrI GCNGC 1 cut(s) 58
Psp6I CCWGG 2 cut(s) 214, 554
PspFI CCCAGC 1 cut(s) 506
PspGI CCWGG 2 cut(s) 214, 554
PspN4I GGNNCC 1 cut(s) 553
PspPI GGNCC 1 cut(s) 54
RsaI GTAC 2 cut(s) 487, 522
RsaNI GTAC 2 cut(s) 486, 521
SatI GCNGC 1 cut(s) 57
Sau3AI GATC 1 cut(s) 398
Sau96I GGNCC 1 cut(s) 54
ScrFI CCNGG 2 cut(s) 216, 556
SetI ASST 7 cut(s) 168, 174, 220, 249, 289, 487, 496
SfaNI GCATC 1 cut(s) 138
SmlI CTYRAG 1 cut(s) 6
SmoI CTYRAG 1 cut(s) 6
SpeI ACTAGT 1 cut(s) 430
Sse9I AATT 2 cut(s) 266, 349
SsiI CCGC 2 cut(s) 57, 127
SspMI CTAG 3 cut(s) 60, 99, 431
StyD4I CCNGG 2 cut(s) 214, 554
TaaI ACNGT 2 cut(s) 518, 525
TaqI TCGA 1 cut(s) 438
TasI AATT 2 cut(s) 266, 349
TatI WGTACW 1 cut(s) 520
TauI GCSGC 1 cut(s) 59
TfiI GAWTC 2 cut(s) 65, 511
TscAI CASTG 2 cut(s) 115, 523
TseFI GTSAC 2 cut(s) 238, 259
Tsp45I GTSAC 2 cut(s) 238, 259
TspDTI ATGAA 2 cut(s) 300, 456
TspGWI ACGGA 1 cut(s) 411
TspRI CASTG 2 cut(s) 115, 523
XagI CCTNNNNNAGG 1 cut(s) 176
XapI RAATTY 1 cut(s) 266
XspI CTAG 3 cut(s) 60, 99, 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.