MD01G1041700.v1.1

Dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0002881
Physical Location & Seq
Reverse (-)
13939849 .. 13942528
2680 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1041700.v1.1.491

Sequence Viewer

Length: 1092 bp
ATGTCTCAAGAAATATTAGTTGCAGCAGAGCAAGCAGTGAAAGCCTATGGATGGGCCGCTAGAGATTCCTCGGGAATTCTCTCTCCGTTCCACTTCATTAGGAGAACTACTGGTAGTAATGATATCAGCATGAAGATACTCTATTGTGGCATTTGCCACTCAGACCTTCACTTAGCTAAGGATGAAATGGGACTGACTATCTATCCCATTGTCCCAGGGCATGAAATTGTAGGGCAAGTGACAAAGGTTGGTAGCAATGTCACAAGATTCCAAGTAGGAGACATAGCTGGAGTTGGGTGCATGGTGGGTTCATGTCGCTCATGCGATAACTGCATACAGGACTTGGAAAATTACTGCCCTAAAATTGTATGGACCTACAACAAACACCATGAAGACGGATCGAGAACCTTTGGTGGATACTCTGACAAACTAGTAGTCGATCAACACTTTGCCGTCCGAATCCCAGATAACTTGTCGTTGCAAGGTACTGCTCCCATGCTCTGTGCCGGGATCACAGTATACAGCCCGATGAGATACTTTGGACTCATGGAGGCTGGTAAGCACTTGGGGGTAGTAGGGCTTGGTGGCCTTGGTCATATGGCCGTGAAGTTTGCAAAAGCTGTTGGAGCAAAAGTGACTGTAATAAGTACTTCTCCGAATAAGAAGAAGGAAGCAATGGAGCAACTTGGTGTTGATGAATTTCTGATTAGTCATGACCAGGAGCAATTGCAGGCTGCCATGGGTACAATGGACGGTATCATCGATACAGTTTCGGCCCCTCACCCTCTCTTGTCCTTGACTGAGATGTTGAAGACCAATGGAAAACTAATTCTGGTGGGTGCTCCAGTACAACCTCCTGAACTTCCAGTTTTCCCCTTAATGTTGGGGAGGAAGCTTATTGCTGGTAGTGCAATTGGGGGAATGAAAGAGACACAAGAGATGATAGATTTTGCTGCAAAGCATAATATCACAGCGGACGTCGAAGTTATTCCGATGGATTACGTCAACACCGCCCTTGAAAGAGTTGCCAAGGCTGATATTAAGTACCGCTTTGTCATTGATGTGGCAAACACTTTGAATTCTCCATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
ADH_N ADH_zinc_N

Protein Analysis

364

Amino Acids

39.3

Weight (kDa)

6.17

Isoelectric Point (pI)

30.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 39 - 156 2.5e-22 Alcohol dehydrogenase GroES-like domain
2-Hacid_dh_C PF02826 180 - 234 9.4e-06 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
AlaDh_PNT_C PF01262 190 - 270 4e-06 Alanine dehydrogenase/PNT, C-terminal domain
ADH_zinc_N PF00107 196 - 319 1.4e-23 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 981
AccI GTMKAC 1 cut(s) 519
AciI CCGC 4 cut(s) 57, 974, 1011, 1048
AclWI GGATC 2 cut(s) 406, 518
AcoI YGGCCR 1 cut(s) 600
AcsI RAATTY 3 cut(s) 75, 698, 1078
AcyI GRCGYC 1 cut(s) 978
AfaI GTAC 5 cut(s) 487, 649, 745, 849, 1046
AfiI CCNNNNNNNGG 1 cut(s) 51
AgsI TTSAA 3 cut(s) 811, 1019, 1078
AhlI ACTAGT 1 cut(s) 430
AjnI CCWGG 2 cut(s) 214, 717
AluBI AGCT 4 cut(s) 176, 287, 620, 895
AluI AGCT 4 cut(s) 176, 287, 620, 895
Alw21I GWGCWC 1 cut(s) 844
Alw26I GTCTC 3 cut(s) 9, 273, 923
AlwI GGATC 2 cut(s) 406, 518
Ama87I CYCGRG 1 cut(s) 70
AoxI GGCC 4 cut(s) 54, 586, 600, 774
ApeKI GCWGC 3 cut(s) 23, 734, 953
ApoI RAATTY 3 cut(s) 75, 698, 1078
ArsI GACNNNNNNTTYG 2 cut(s) 420, 452
Asp700I GAANNNNTTC 1 cut(s) 987
AspS9I GGNCC 3 cut(s) 54, 372, 775
AsuC2I CCSGG 1 cut(s) 508
AsuHPI GGTGA 1 cut(s) 773
AvaI CYCGRG 1 cut(s) 70
AvaII GGWCC 1 cut(s) 372
BbsI GAAGAC 2 cut(s) 399, 818
Bbv12I GWGCWC 1 cut(s) 844
BbvI GCAGC 3 cut(s) 35, 721, 940
BccI CCATC 2 cut(s) 45, 988
BceAI ACGGC 2 cut(s) 437, 587
BciT130I CCWGG 2 cut(s) 216, 719
BciVI GTATCC 1 cut(s) 410
BcnI CCSGG 1 cut(s) 508
BcoDI GTCTC 3 cut(s) 9, 273, 923
BcuI ACTAGT 1 cut(s) 430
BfaI CTAG 2 cut(s) 60, 431
BfuI GTATCC 1 cut(s) 410
BisI GCNGC 4 cut(s) 24, 57, 735, 954
BlsI GCNGC 4 cut(s) 25, 58, 736, 955
BmcAI AGTACT 1 cut(s) 649
Bme1390I CCNGG 3 cut(s) 216, 508, 719
Bme18I GGWCC 1 cut(s) 372
BmeT110I CYCGRG 1 cut(s) 70
BmgT120I GGNCC 3 cut(s) 54, 372, 775
BmiI GGNNCC 1 cut(s) 777
BmrFI CCNGG 3 cut(s) 216, 508, 719
BpiI GAAGAC 2 cut(s) 399, 818
BpmI CTGGAG 2 cut(s) 309, 828
Bpu10I CCTNAGC 1 cut(s) 177
BpuMI CCSGG 1 cut(s) 508
Bsa29I ATCGAT 1 cut(s) 762
BsaHI GRCGYC 1 cut(s) 978
BsaJI CCNNGG 6 cut(s) 69, 214, 215, 589, 738, 1029
BsaXI ACNNNNNCTCC 2 cut(s) 618, 648
Bsc4I CCNNNNNNNGG 1 cut(s) 51
Bse1I ACTGG 3 cut(s) 115, 845, 866
Bse3DI GCAATG 2 cut(s) 262, 681
BseBI CCWGG 2 cut(s) 216, 719
BseCI ATCGAT 1 cut(s) 762
BseDI CCNNGG 6 cut(s) 69, 214, 215, 589, 738, 1029
BseGI GGATG 2 cut(s) 56, 187
BseLI CCNNNNNNNGG 1 cut(s) 51
BseMI GCAATG 2 cut(s) 262, 681
BseMII CTCAG 2 cut(s) 174, 792
BseNI ACTGG 3 cut(s) 115, 845, 866
BseXI GCAGC 3 cut(s) 35, 721, 940
BshFI GGCC 4 cut(s) 56, 588, 602, 776
BshVI ATCGAT 1 cut(s) 762
BsiHKAI GWGCWC 1 cut(s) 844
BsiHKCI CYCGRG 1 cut(s) 70
BsiSI CCGG 1 cut(s) 507
BslFI GGGAC 2 cut(s) 197, 204
BslI CCNNNNNNNGG 1 cut(s) 51
BsmAI GTCTC 3 cut(s) 9, 273, 923
BsmFI GGGAC 2 cut(s) 197, 204
BsnI GGCC 4 cut(s) 56, 588, 602, 776
BsoBI CYCGRG 1 cut(s) 70
Bsp1286I GDGCHC 1 cut(s) 844
Bsp143I GATC 3 cut(s) 398, 439, 510
Bsp19I CCATGG 1 cut(s) 738
BspACI CCGC 4 cut(s) 57, 974, 1011, 1048
BspANI GGCC 4 cut(s) 56, 588, 602, 776
BspCNI CTCAG 2 cut(s) 173, 793
BspDI ATCGAT 1 cut(s) 762
BspHI TCATGA 1 cut(s) 712
BspLI GGNNCC 1 cut(s) 777
BspPI GGATC 2 cut(s) 406, 518
BsrDI GCAATG 2 cut(s) 262, 681
BsrI ACTGG 3 cut(s) 115, 845, 866
BssECI CCNNGG 6 cut(s) 69, 214, 215, 589, 738, 1029
BssMI GATC 3 cut(s) 398, 439, 510
BssNAI GTATAC 1 cut(s) 520
BssNI GRCGYC 1 cut(s) 978
BssT1I CCWWGG 3 cut(s) 589, 738, 1029
Bst1107I GTATAC 1 cut(s) 520
Bst2UI CCWGG 2 cut(s) 216, 719
Bst4CI ACNGT 4 cut(s) 517, 640, 755, 769
BstACI GRCGYC 1 cut(s) 978
BstC8I GCNNGC 2 cut(s) 33, 732
BstDEI CTNAG 4 cut(s) 160, 172, 177, 801
BstDSI CCRYGG 1 cut(s) 738
BstF5I GGATG 2 cut(s) 56, 187
BstKTI GATC 3 cut(s) 401, 442, 513
BstMAI GTCTC 3 cut(s) 9, 273, 923
BstMBI GATC 3 cut(s) 398, 439, 510
BstMWI GCNNNNNNNGC 5 cut(s) 32, 41, 330, 626, 908
BstNI CCWGG 2 cut(s) 216, 719
BstSCI CCNGG 3 cut(s) 214, 506, 717
BstV1I GCAGC 3 cut(s) 35, 721, 940
BstV2I GAAGAC 2 cut(s) 399, 818
BstZ17I GTATAC 1 cut(s) 520
Bsu15I ATCGAT 1 cut(s) 762
BsuI GTATCC 1 cut(s) 410
BsuRI GGCC 4 cut(s) 56, 588, 602, 776
BsuTUI ATCGAT 1 cut(s) 762
BtgI CCRYGG 1 cut(s) 738
BtsCI GGATG 2 cut(s) 56, 187
BtsI GCAGTG 1 cut(s) 42
BtsIMutI CAGTG 1 cut(s) 42
Cac8I GCNNGC 2 cut(s) 33, 732
CciI TCATGA 1 cut(s) 712
Cfr13I GGNCC 3 cut(s) 54, 372, 775
ClaI ATCGAT 1 cut(s) 762
Csp6I GTAC 5 cut(s) 486, 648, 744, 848, 1045
CviQI GTAC 5 cut(s) 486, 648, 744, 848, 1045
DdeI CTNAG 4 cut(s) 160, 172, 177, 801
DpnI GATC 3 cut(s) 400, 441, 512
DpnII GATC 3 cut(s) 398, 439, 510
EaeI YGGCCR 1 cut(s) 600
Eco130I CCWWGG 3 cut(s) 589, 738, 1029
Eco32I GATATC 1 cut(s) 124
Eco47I GGWCC 1 cut(s) 372
Eco88I CYCGRG 1 cut(s) 70
EcoRI GAATTC 2 cut(s) 75, 1078
EcoRII CCWGG 2 cut(s) 214, 717
EcoRV GATATC 1 cut(s) 124
EcoT14I CCWWGG 3 cut(s) 589, 738, 1029
ErhI CCWWGG 3 cut(s) 589, 738, 1029
FalI AAGNNNNNCTT 2 cut(s) 1034, 1066
FaqI GGGAC 2 cut(s) 197, 204
FauNDI CATATG 1 cut(s) 597
FblI GTMKAC 1 cut(s) 519
Fnu4HI GCNGC 4 cut(s) 24, 57, 735, 954
FokI GGATG 2 cut(s) 63, 194
Fsp4HI GCNGC 4 cut(s) 24, 57, 735, 954
FspBI CTAG 2 cut(s) 60, 431
GluI GCNGC 4 cut(s) 24, 57, 735, 954
GsuI CTGGAG 2 cut(s) 309, 828
HaeIII GGCC 4 cut(s) 56, 588, 602, 776
HapII CCGG 1 cut(s) 507
Hin1I GRCGYC 1 cut(s) 978
HincII GTYRAC 1 cut(s) 1006
HindII GTYRAC 1 cut(s) 1006
HindIII AAGCTT 1 cut(s) 893
HinfI GANTC 4 cut(s) 65, 267, 459, 543
HpaII CCGG 1 cut(s) 507
HphI GGTGA 1 cut(s) 773
Hpy166II GTNNAC 2 cut(s) 520, 1006
Hpy188I TCNGA 6 cut(s) 163, 424, 458, 657, 705, 993
Hpy188III TCNNGA 5 cut(s) 8, 72, 402, 713, 857
Hpy8I GTNNAC 2 cut(s) 520, 1006
Hpy99I CGWCG 1 cut(s) 983
HpyAV CCTTC 2 cut(s) 176, 661
HpyCH4III ACNGT 4 cut(s) 517, 640, 755, 769
HpyCH4IV ACGT 2 cut(s) 978, 1002
HpyCH4V TGCA 8 cut(s) 23, 300, 333, 481, 614, 730, 911, 956
HpyF10VI GCNNNNNNNGC 5 cut(s) 32, 41, 330, 626, 908
HpyF3I CTNAG 4 cut(s) 160, 172, 177, 801
HpySE526I ACGT 2 cut(s) 978, 1002
Hsp92I GRCGYC 1 cut(s) 978
Kzo9I GATC 3 cut(s) 398, 439, 510
LmnI GCTCC 5 cut(s) 496, 626, 679, 721, 847
Lsp1109I GCAGC 3 cut(s) 35, 721, 940
MaeI CTAG 2 cut(s) 60, 431
MaeII ACGT 2 cut(s) 978, 1002
MaeIII GTNAC 3 cut(s) 238, 259, 634
MalI GATC 3 cut(s) 400, 441, 512
MboI GATC 3 cut(s) 398, 439, 510
MboII GAAGA 4 cut(s) 145, 404, 676, 823
MfeI CAATTG 2 cut(s) 725, 912
MhlI GDGCHC 1 cut(s) 844
MluCI AATT 9 cut(s) 75, 225, 349, 363, 698, 725, 828, 912, 1078
MlyI GAGTC 1 cut(s) 537
MmeI TCCRAC 1 cut(s) 604
MnlI CCTC 6 cut(s) 79, 544, 789, 795, 864, 882
MroXI GAANNNNTTC 1 cut(s) 987
MseI TTAA 2 cut(s) 878, 1041
MslI CAYNNNNRTG 1 cut(s) 1061
MspA1I CMGCKG 1 cut(s) 974
MspI CCGG 1 cut(s) 507
MspR9I CCNGG 3 cut(s) 216, 508, 719
MunI CAATTG 2 cut(s) 725, 912
MvaI CCWGG 2 cut(s) 216, 719
MwoI GCNNNNNNNGC 5 cut(s) 32, 41, 330, 626, 908
NciI CCSGG 1 cut(s) 508
NcoI CCATGG 1 cut(s) 738
NdeI CATATG 1 cut(s) 597
NdeII GATC 3 cut(s) 398, 439, 510
NlaIV GGNNCC 1 cut(s) 777
NmuCI GTSAC 3 cut(s) 238, 259, 634
PagI TCATGA 1 cut(s) 712
PasI CCCWGGG 1 cut(s) 215
PcsI WCGNNNNNNNCGW 1 cut(s) 759
PdmI GAANNNNTTC 1 cut(s) 987
PfeI GAWTC 3 cut(s) 65, 267, 459
PkrI GCNGC 4 cut(s) 25, 58, 736, 955
PleI GAGTC 1 cut(s) 537
PpsI GAGTC 1 cut(s) 537
Psp6I CCWGG 2 cut(s) 214, 717
PspGI CCWGG 2 cut(s) 214, 717
PspN4I GGNNCC 1 cut(s) 777
PspPI GGNCC 3 cut(s) 54, 372, 775
RsaI GTAC 5 cut(s) 487, 649, 745, 849, 1046
RsaNI GTAC 5 cut(s) 486, 648, 744, 848, 1045
RseI CAYNNNNRTG 1 cut(s) 1061
SaqAI TTAA 2 cut(s) 878, 1041
SatI GCNGC 4 cut(s) 24, 57, 735, 954
Sau3AI GATC 3 cut(s) 398, 439, 510
Sau96I GGNCC 3 cut(s) 54, 372, 775
ScaI AGTACT 1 cut(s) 649
SchI GAGTC 1 cut(s) 537
ScrFI CCNGG 3 cut(s) 216, 508, 719
SduI GDGCHC 1 cut(s) 844
SinI GGWCC 1 cut(s) 372
SmiMI CAYNNNNRTG 1 cut(s) 1061
SmlI CTYRAG 1 cut(s) 6
SmoI CTYRAG 1 cut(s) 6
SpeI ACTAGT 1 cut(s) 430
Sse9I AATT 9 cut(s) 75, 225, 349, 363, 698, 725, 828, 912, 1078
SsiI CCGC 4 cut(s) 57, 974, 1011, 1048
SspI AATATT 1 cut(s) 15
SspMI CTAG 2 cut(s) 60, 431
StyD4I CCNGG 3 cut(s) 214, 506, 717
StyI CCWWGG 3 cut(s) 589, 738, 1029
TaaI ACNGT 4 cut(s) 517, 640, 755, 769
TaiI ACGT 2 cut(s) 981, 1005
TaqI TCGA 4 cut(s) 401, 438, 762, 981
TasI AATT 9 cut(s) 75, 225, 349, 363, 698, 725, 828, 912, 1078
TatI WGTACW 2 cut(s) 647, 847
TauI GCSGC 1 cut(s) 59
TfiI GAWTC 3 cut(s) 65, 267, 459
Tru1I TTAA 2 cut(s) 878, 1041
Tru9I TTAA 2 cut(s) 878, 1041
TscAI CASTG 1 cut(s) 42
TseFI GTSAC 3 cut(s) 238, 259, 634
TseI GCWGC 3 cut(s) 23, 734, 953
Tsp45I GTSAC 3 cut(s) 238, 259, 634
TspDTI ATGAA 8 cut(s) 85, 146, 198, 237, 300, 405, 711, 938
TspGWI ACGGA 2 cut(s) 75, 411
TspRI CASTG 1 cut(s) 42
VpaK11BI GGWCC 1 cut(s) 372
XapI RAATTY 3 cut(s) 75, 698, 1078
XcmI CCANNNNNNNNNTGG 1 cut(s) 745
XmiI GTMKAC 1 cut(s) 519
XmnI GAANNNNTTC 1 cut(s) 987
XspI CTAG 2 cut(s) 60, 431
ZraI GACGTC 1 cut(s) 979
ZrmI AGTACT 1 cut(s) 649
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.