Rh2CG171700

Dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0002881
Physical Location & Seq
Reverse (-)
15356958 .. 15359062
2105 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG171700.1

Sequence Viewer

Length: 792 bp
ATGGTGGGTTCATGTGGTTCGTGCGGTAACTGCACACGAGATTTGGAAAATTACTGTCCCAAAATGGTATGGACCTACAACAAACACTACCATGACGGATCGATAACTTTCGGTGGATACTCCGACAAATTTGTAGTCGAGGAGCATTTCGCAGTACGAATCCCAGATAACTTGCCATTGGAGGGTGTTGCACCTATGCTTTGTGCTGGCATTACGGTGTACAGCCCTATGAGACACTTTGGACTACTTGAGCATGGTAAGCACTTGGGTGTGGTAGGGCTTGGGGGCCTCGGTCATATGGCAGTGAAGTTTGCCAAGGCTATCGGAGTAAAAGTGACTGTGATCAGTACCTCTCCAAACAAGAAGAAGGAAGCAATGGAACAACTTGGTGTAGACCATTTTCTGATCAGTCGCAACCAAGAGGAAATGCAGGCTGCCATGGGTACGTTGGACGGTATCATCGATACAGTTTCAGCTCCTCATTCTCTCACGCCATTGGTTGACTTATTAACGACGGATGGAAATCTAATCGTGGTGGGTGCTCCGCTCCAACCACCTGAGCTACCGGTGTTTCCTTTGATACTTGGGAGAAAGTCTATTGCCGGGAGTGCAATTGGGGGAATGAAAGAGACTCAAGAGATGATAGATTTTGCAGCAAAGCATAATATCACAGCTGATGTTGAAGTCATTCCTATGGATTATGTGAACACTGCTTTTGAACGAGTTGCCAAGTTAGATGTTAAGTACCGATTTGTCATTGACGTCGCAAACACCCTGAATTCTCCATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
ADH_N ADH_zinc_N

Protein Analysis

263

Amino Acids

28.51

Weight (kDa)

5.91

Isoelectric Point (pI)

30.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh_C PF02826 84 - 130 1e-05 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
AlaDh_PNT_C PF01262 90 - 166 3.6e-06 Alanine dehydrogenase/PNT, C-terminal domain
ADH_zinc_N PF00107 96 - 219 1.9e-25 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 765
AccBSI CCGCTC 1 cut(s) 547
AccI GTMKAC 1 cut(s) 393
AciI CCGC 2 cut(s) 24, 545
AclWI GGATC 1 cut(s) 106
AcsI RAATTY 2 cut(s) 128, 778
AcyI GRCGYC 1 cut(s) 762
AfaI GTAC 5 cut(s) 156, 221, 349, 445, 746
AfiI CCNNNNNNNGG 1 cut(s) 182
AgeI ACCGGT 1 cut(s) 565
AgsI TTSAA 2 cut(s) 683, 719
AleI CACNNNNGTG 1 cut(s) 267
AluBI AGCT 3 cut(s) 476, 562, 674
AluI AGCT 3 cut(s) 476, 562, 674
Alw21I GWGCWC 1 cut(s) 544
Alw26I GTCTC 2 cut(s) 226, 623
AlwI GGATC 1 cut(s) 106
AoxI GGCC 1 cut(s) 286
ApeKI GCWGC 2 cut(s) 434, 653
ApoI RAATTY 2 cut(s) 128, 778
ArsI GACNNNNNNTTYG 2 cut(s) 120, 152
AsiGI ACCGGT 1 cut(s) 565
Asp700I GAANNNNTTC 1 cut(s) 687
AspS9I GGNCC 2 cut(s) 72, 286
AsuC2I CCSGG 1 cut(s) 604
AvaII GGWCC 1 cut(s) 72
BauI CACGAG 1 cut(s) 36
Bbv12I GWGCWC 1 cut(s) 544
BbvI GCAGC 2 cut(s) 421, 665
BccI CCATC 1 cut(s) 512
BciVI GTATCC 1 cut(s) 110
BclI TGATCA 2 cut(s) 342, 405
BcnI CCSGG 1 cut(s) 604
BcoDI GTCTC 2 cut(s) 226, 623
BfuI GTATCC 1 cut(s) 110
BisI GCNGC 2 cut(s) 435, 654
BlsI GCNGC 2 cut(s) 436, 655
Bme1390I CCNGG 1 cut(s) 604
Bme18I GGWCC 1 cut(s) 72
BmgT120I GGNCC 2 cut(s) 72, 286
BmiI GGNNCC 1 cut(s) 287
BmrFI CCNGG 1 cut(s) 604
Bpu10I CCTNAGC 1 cut(s) 558
BpuEI CTTGAG 2 cut(s) 269, 618
BpuMI CCSGG 1 cut(s) 604
Bsa29I ATCGAT 2 cut(s) 101, 462
BsaBI GATNNNNATC 1 cut(s) 522
BsaHI GRCGYC 1 cut(s) 762
BsaJI CCNNGG 3 cut(s) 289, 315, 438
BsaWI WCCGGW 1 cut(s) 565
BsaXI ACNNNNNCTCC 2 cut(s) 318, 348
Bsc4I CCNNNNNNNGG 1 cut(s) 182
Bse118I RCCGGY 1 cut(s) 565
Bse3DI GCAATG 1 cut(s) 381
Bse8I GATNNNNATC 1 cut(s) 522
BseCI ATCGAT 2 cut(s) 101, 462
BseDI CCNNGG 3 cut(s) 289, 315, 438
BseGI GGATG 1 cut(s) 523
BseJI GATNNNNATC 1 cut(s) 522
BseLI CCNNNNNNNGG 1 cut(s) 182
BseMI GCAATG 1 cut(s) 381
BseMII CTCAG 1 cut(s) 549
BseRI GAGGAG 2 cut(s) 155, 468
BseXI GCAGC 2 cut(s) 421, 665
BsgI GTGCAG 1 cut(s) 16
BshFI GGCC 1 cut(s) 288
BshTI ACCGGT 1 cut(s) 565
BshVI ATCGAT 2 cut(s) 101, 462
BsiHKAI GWGCWC 1 cut(s) 544
BsiSI CCGG 2 cut(s) 566, 603
BslFI GGGAC 1 cut(s) 42
BslI CCNNNNNNNGG 1 cut(s) 182
BsmAI GTCTC 2 cut(s) 226, 623
BsmFI GGGAC 1 cut(s) 42
BsnI GGCC 1 cut(s) 288
Bsp1286I GDGCHC 1 cut(s) 544
Bsp1407I TGTACA 1 cut(s) 219
Bsp143I GATC 3 cut(s) 98, 342, 405
Bsp19I CCATGG 1 cut(s) 438
BspACI CCGC 2 cut(s) 24, 545
BspANI GGCC 1 cut(s) 288
BspCNI CTCAG 1 cut(s) 550
BspDI ATCGAT 2 cut(s) 101, 462
BspLI GGNNCC 1 cut(s) 287
BspPI GGATC 1 cut(s) 106
BsrBI CCGCTC 1 cut(s) 547
BsrDI GCAATG 1 cut(s) 381
BsrFI RCCGGY 1 cut(s) 565
BsrGI TGTACA 1 cut(s) 219
BssAI RCCGGY 1 cut(s) 565
BssECI CCNNGG 3 cut(s) 289, 315, 438
BssMI GATC 3 cut(s) 98, 342, 405
BssNI GRCGYC 1 cut(s) 762
BssSI CACGAG 1 cut(s) 36
BssT1I CCWWGG 2 cut(s) 315, 438
Bst2BI CACGAG 1 cut(s) 36
Bst4CI ACNGT 5 cut(s) 56, 217, 340, 455, 469
BstACI GRCGYC 1 cut(s) 762
BstAUI TGTACA 1 cut(s) 219
BstC8I GCNNGC 2 cut(s) 208, 432
BstDEI CTNAG 1 cut(s) 558
BstDSI CCRYGG 1 cut(s) 438
BstF5I GGATG 1 cut(s) 523
BstKTI GATC 3 cut(s) 101, 345, 408
BstMAI GTCTC 2 cut(s) 226, 623
BstMBI GATC 3 cut(s) 98, 342, 405
BstMWI GCNNNNNNNGC 3 cut(s) 30, 259, 608
BstSCI CCNGG 1 cut(s) 602
BstV1I GCAGC 2 cut(s) 421, 665
Bsu15I ATCGAT 2 cut(s) 101, 462
BsuI GTATCC 1 cut(s) 110
BsuRI GGCC 1 cut(s) 288
BsuTUI ATCGAT 2 cut(s) 101, 462
BtgI CCRYGG 1 cut(s) 438
BtsCI GGATG 1 cut(s) 523
BtsI GCAGTG 2 cut(s) 309, 708
BtsIMutI CAGTG 2 cut(s) 309, 708
Cac8I GCNNGC 2 cut(s) 208, 432
Cfr10I RCCGGY 1 cut(s) 565
Cfr13I GGNCC 2 cut(s) 72, 286
ClaI ATCGAT 2 cut(s) 101, 462
Csp6I GTAC 5 cut(s) 155, 220, 348, 444, 745
CspAI ACCGGT 1 cut(s) 565
CviAII CATG 4 cut(s) 12, 92, 254, 439
CviJI RGCY 8 cut(s) 225, 280, 288, 320, 434, 476, 562, 674
CviKI_1 RGCY 8 cut(s) 225, 280, 288, 320, 434, 476, 562, 674
CviQI GTAC 5 cut(s) 155, 220, 348, 444, 745
DdeI CTNAG 1 cut(s) 558
DpnI GATC 3 cut(s) 100, 344, 407
DpnII GATC 3 cut(s) 98, 342, 405
Eco130I CCWWGG 2 cut(s) 315, 438
Eco47I GGWCC 1 cut(s) 72
EcoO109I RGGNCCY 1 cut(s) 286
EcoRI GAATTC 1 cut(s) 778
EcoT14I CCWWGG 2 cut(s) 315, 438
ErhI CCWWGG 2 cut(s) 315, 438
FaeI CATG 4 cut(s) 15, 95, 257, 442
FaqI GGGAC 1 cut(s) 42
FatI CATG 4 cut(s) 11, 91, 253, 438
FauNDI CATATG 1 cut(s) 297
FbaI TGATCA 2 cut(s) 342, 405
FblI GTMKAC 1 cut(s) 393
Fnu4HI GCNGC 2 cut(s) 435, 654
FokI GGATG 1 cut(s) 530
Fsp4HI GCNGC 2 cut(s) 435, 654
GluI GCNGC 2 cut(s) 435, 654
HaeIII GGCC 1 cut(s) 288
HapII CCGG 2 cut(s) 566, 603
Hin1I GRCGYC 1 cut(s) 762
Hin1II CATG 4 cut(s) 15, 95, 257, 442
HincII GTYRAC 1 cut(s) 502
HindII GTYRAC 1 cut(s) 502
HinfI GANTC 2 cut(s) 159, 631
HpaII CCGG 2 cut(s) 566, 603
Hpy166II GTNNAC 4 cut(s) 220, 394, 502, 706
Hpy188I TCNGA 3 cut(s) 124, 326, 405
Hpy188III TCNNGA 1 cut(s) 635
Hpy8I GTNNAC 4 cut(s) 220, 394, 502, 706
Hpy99I CGWCG 2 cut(s) 517, 767
HpyAV CCTTC 1 cut(s) 361
HpyCH4III ACNGT 5 cut(s) 56, 217, 340, 455, 469
HpyCH4IV ACGT 2 cut(s) 446, 762
HpyCH4V TGCA 5 cut(s) 33, 191, 430, 611, 653
HpyF10VI GCNNNNNNNGC 3 cut(s) 30, 259, 608
HpyF3I CTNAG 1 cut(s) 558
HpySE526I ACGT 2 cut(s) 446, 762
Hsp92I GRCGYC 1 cut(s) 762
Hsp92II CATG 4 cut(s) 15, 95, 257, 442
Ksp22I TGATCA 2 cut(s) 342, 405
Kzo9I GATC 3 cut(s) 98, 342, 405
LmnI GCTCC 4 cut(s) 142, 481, 547, 552
LpnPI CCDG 7 cut(s) 177, 192, 416, 570, 579, 616, 788
Lsp1109I GCAGC 2 cut(s) 421, 665
MaeII ACGT 2 cut(s) 446, 762
MaeIII GTNAC 2 cut(s) 26, 334
MalI GATC 3 cut(s) 100, 344, 407
MbiI CCGCTC 1 cut(s) 547
MboI GATC 3 cut(s) 98, 342, 405
MboII GAAGA 1 cut(s) 376
MfeI CAATTG 1 cut(s) 612
MhlI GDGCHC 1 cut(s) 544
MluCI AATT 4 cut(s) 49, 128, 612, 778
MlyI GAGTC 1 cut(s) 625
MmeI TCCRAC 3 cut(s) 147, 429, 574
MnlI CCTC 6 cut(s) 133, 175, 299, 361, 415, 489
MroXI GAANNNNTTC 1 cut(s) 687
MseI TTAA 2 cut(s) 509, 741
MslI CAYNNNNRTG 4 cut(s) 90, 215, 267, 692
MspA1I CMGCKG 1 cut(s) 674
MspI CCGG 2 cut(s) 566, 603
MspR9I CCNGG 1 cut(s) 604
MunI CAATTG 1 cut(s) 612
MwoI GCNNNNNNNGC 3 cut(s) 30, 259, 608
NciI CCSGG 1 cut(s) 604
NcoI CCATGG 1 cut(s) 438
NdeI CATATG 1 cut(s) 297
NdeII GATC 3 cut(s) 98, 342, 405
NlaIII CATG 4 cut(s) 15, 95, 257, 442
NlaIV GGNNCC 1 cut(s) 287
NmuCI GTSAC 1 cut(s) 334
OliI CACNNNNGTG 1 cut(s) 267
PcsI WCGNNNNNNNCGW 1 cut(s) 459
PdmI GAANNNNTTC 1 cut(s) 687
PfeI GAWTC 1 cut(s) 159
PinAI ACCGGT 1 cut(s) 565
PkrI GCNGC 2 cut(s) 436, 655
PleI GAGTC 1 cut(s) 625
PpsI GAGTC 1 cut(s) 625
PspN4I GGNNCC 1 cut(s) 287
PspPI GGNCC 2 cut(s) 72, 286
PvuII CAGCTG 1 cut(s) 674
RsaI GTAC 5 cut(s) 156, 221, 349, 445, 746
RsaNI GTAC 5 cut(s) 155, 220, 348, 444, 745
RseI CAYNNNNRTG 4 cut(s) 90, 215, 267, 692
SaqAI TTAA 2 cut(s) 509, 741
SatI GCNGC 2 cut(s) 435, 654
Sau3AI GATC 3 cut(s) 98, 342, 405
Sau96I GGNCC 2 cut(s) 72, 286
SchI GAGTC 1 cut(s) 625
ScrFI CCNGG 1 cut(s) 604
SduI GDGCHC 1 cut(s) 544
SetI ASST 9 cut(s) 77, 196, 353, 449, 478, 559, 564, 676, 765
SinI GGWCC 1 cut(s) 72
SmiMI CAYNNNNRTG 4 cut(s) 90, 215, 267, 692
SmlI CTYRAG 2 cut(s) 248, 633
SmoI CTYRAG 2 cut(s) 248, 633
Sse9I AATT 4 cut(s) 49, 128, 612, 778
SsiI CCGC 2 cut(s) 24, 545
StyD4I CCNGG 1 cut(s) 602
StyI CCWWGG 2 cut(s) 315, 438
TaaI ACNGT 5 cut(s) 56, 217, 340, 455, 469
TaiI ACGT 2 cut(s) 449, 765
TaqI TCGA 3 cut(s) 101, 138, 462
TaqII GACCGA 1 cut(s) 281
TasI AATT 4 cut(s) 49, 128, 612, 778
TatI WGTACW 1 cut(s) 219
TfiI GAWTC 1 cut(s) 159
Tru1I TTAA 2 cut(s) 509, 741
Tru9I TTAA 2 cut(s) 509, 741
TscAI CASTG 2 cut(s) 309, 715
TseFI GTSAC 1 cut(s) 334
TseI GCWGC 2 cut(s) 434, 653
Tsp45I GTSAC 1 cut(s) 334
TspDTI ATGAA 1 cut(s) 638
TspGWI ACGGA 2 cut(s) 111, 530
TspRI CASTG 2 cut(s) 309, 715
VpaK11BI GGWCC 1 cut(s) 72
XapI RAATTY 2 cut(s) 128, 778
XcmI CCANNNNNNNNNTGG 1 cut(s) 445
XmiI GTMKAC 1 cut(s) 393
XmnI GAANNNNTTC 1 cut(s) 687
ZraI GACGTC 1 cut(s) 763
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.