Rw2G017020

Dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0002881
Physical Location & Seq
Forward (+)
20732213 .. 20735054
2842 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G017020.1

Sequence Viewer

Length: 1098 bp
ATGTCTAAAGCACCAGCTCAAGAAGAAGCAGTGCATGCTTATGGATGGGCTGCTAGAGATTCCTCAGGAATTCTTTCCCCCTACCATTTCACTAGAAGGGCTAACGGGGATAATGATATCACCATCAAGATACAATATTGCGGAATTTGCCATGGAGACATTCACCTCGTAAAGAACAAAGTATGGTCGACCATTTATCCGACTGTGCCTGGGCATGAAATTGTTGGCCAAGTGACCAAGGTTGGGCTCAACGTCAAAAAATTCAAAGTTGGAGATGTAGCCGGAGTAGGGTGCATGATGGGTTCATGTGGCTCATGCGAGAACTGCAAACAAGACTTGGAAAATTACTGCCCCAAAATGTTATGGACCTTCAGCGGACAATATGAAGATGGAACAAGAGCCTTTGGTGGATATTCAGATAAGTTTGTTGTGGAAGAGCACTTTGCCGTCCGAATCCCAAAAGACATTCCACTAGCAGGTGCCGCCCCGCTATTGTGTGCTGGGATTACCGTGTATAGCCCCCTGAAATATTTCGGACTAGTCCCCCAGCCCGGTAGCAAGAAACATTTGGGTATTGTTGGGCTTGGAGGTGTAGGTCATATGGCCGTGAAGTTTGCCAAAGCTTTCGGAGCAAAGGTCACTGTGATTAGTTCCTCTCCGAGTAAGGAAAAGGAAGCAGTGGAACGACTTGGTGCAGATTCATTTTTGATCAGTCATGACCATGAGAAATTGGAGGCTGCCTTGGGCACCATGGATGGTATCATTGACACTGTTTCTGCTTCTCACTCTCTCCTCCCATTGATTGGCTTGCTGAAAACTAGTGGAAAACTAGTTTTGGTGGGTGCTCCAGTGGAGCCACCTGAGCTTCCCGTCTTCCCTTTAATTATGGGGAGGAAAGTTATTGCTGGTAGTGCAATTGGGGGAATGAAAGAGACTCAAGAGATGATAGATTTTGCCGCAAAGCACAACATCACAGCAGATGTCGAAGTTATTCCAATGGATTATGTGAACACTGCTCTTGAACGACTTGAGAAGGGCGACAATGTTAAATATCGCTTTGTCATCAATGTTGCCAACACGATAAAATGTGCAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
ADH_N ADH_zinc_N

Protein Analysis

365

Amino Acids

39.17

Weight (kDa)

7.6

Isoelectric Point (pI)

28.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 36 - 154 1.1e-24 Alcohol dehydrogenase GroES-like domain
2-Hacid_dh_C PF02826 186 - 229 3.5e-06 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
AlaDh_PNT_C PF01262 190 - 260 4e-06 Alanine dehydrogenase/PNT, C-terminal domain
ADH_zinc_N PF00107 197 - 320 1.1e-22 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 467
Acc36I ACCTGC 1 cut(s) 467
AccB1I GGYRCC 2 cut(s) 479, 746
AccB7I CCANNNNNTGG 1 cut(s) 803
AccI GTMKAC 1 cut(s) 188
AciI CCGC 5 cut(s) 141, 375, 483, 488, 957
AcoI YGGCCR 2 cut(s) 226, 603
AcsI RAATTY 3 cut(s) 69, 144, 260
AcuI CTGAAG 1 cut(s) 355
AfiI CCNNNNNNNGG 5 cut(s) 243, 288, 476, 551, 803
AgsI TTSAA 2 cut(s) 265, 1022
AhlI ACTAGT 3 cut(s) 538, 818, 829
AjnI CCWGG 1 cut(s) 208
AluBI AGCT 3 cut(s) 17, 623, 865
AluI AGCT 3 cut(s) 17, 623, 865
Alw21I GWGCWC 2 cut(s) 441, 847
Alw26I GTCTC 2 cut(s) 150, 926
AoxI GGCC 2 cut(s) 226, 603
ApeKI GCWGC 2 cut(s) 50, 737
ApoI RAATTY 3 cut(s) 69, 144, 260
Asp700I GAANNNNTTC 3 cut(s) 73, 530, 990
AspS9I GGNCC 1 cut(s) 366
AsuC2I CCSGG 1 cut(s) 552
AsuHPI GGTGA 2 cut(s) 112, 155
AvaII GGWCC 1 cut(s) 366
AxyI CCTNAGG 1 cut(s) 64
BaeGI GKGCMC 1 cut(s) 749
BalI TGGCCA 1 cut(s) 228
BanI GGYRCC 2 cut(s) 479, 746
BanII GRGCYC 1 cut(s) 249
BbsI GAAGAC 1 cut(s) 865
Bbv12I GWGCWC 2 cut(s) 441, 847
BbvI GCAGC 2 cut(s) 37, 724
BccI CCATC 5 cut(s) 39, 131, 292, 383, 749
BceAI ACGGC 2 cut(s) 431, 590
BciT130I CCWGG 1 cut(s) 210
BclI TGATCA 1 cut(s) 708
BcnI CCSGG 1 cut(s) 552
BcoDI GTCTC 2 cut(s) 150, 926
BcuI ACTAGT 3 cut(s) 538, 818, 829
BfaI CTAG 7 cut(s) 54, 93, 473, 539, 819, 830, 1096
BfuAI ACCTGC 1 cut(s) 467
BisI GCNGC 4 cut(s) 51, 483, 738, 957
BlsI GCNGC 4 cut(s) 52, 484, 739, 958
Bme1390I CCNGG 2 cut(s) 210, 552
Bme18I GGWCC 1 cut(s) 366
BmgT120I GGNCC 1 cut(s) 366
BmiI GGNNCC 3 cut(s) 481, 748, 855
BmrFI CCNGG 2 cut(s) 210, 552
BpiI GAAGAC 1 cut(s) 865
BpmI CTGGAG 1 cut(s) 831
Bpu10I CCTNAGC 1 cut(s) 861
BpuEI CTTGAG 2 cut(s) 921, 1049
BpuMI CCSGG 1 cut(s) 552
BsaJI CCNNGG 5 cut(s) 151, 209, 237, 741, 750
BsaXI ACNNNNNCTCC 2 cut(s) 621, 651
Bsc4I CCNNNNNNNGG 5 cut(s) 243, 288, 476, 551, 803
Bse1I ACTGG 1 cut(s) 848
Bse21I CCTNAGG 1 cut(s) 64
BseBI CCWGG 1 cut(s) 210
BseDI CCNNGG 5 cut(s) 151, 209, 237, 741, 750
BseGI GGATG 2 cut(s) 50, 760
BseLI CCNNNNNNNGG 5 cut(s) 243, 288, 476, 551, 803
BseMII CTCAG 2 cut(s) 78, 852
BseNI ACTGG 1 cut(s) 848
BseRI GAGGAG 1 cut(s) 782
BseSI GKGCMC 1 cut(s) 749
BseXI GCAGC 2 cut(s) 37, 724
BseYI CCCAGC 2 cut(s) 500, 546
BsgI GTGCAG 1 cut(s) 714
BshFI GGCC 2 cut(s) 228, 605
BshNI GGYRCC 2 cut(s) 479, 746
BsiHKAI GWGCWC 2 cut(s) 441, 847
BsiSI CCGG 2 cut(s) 282, 552
BslFI GGGAC 1 cut(s) 527
BslI CCNNNNNNNGG 5 cut(s) 243, 288, 476, 551, 803
BsmAI GTCTC 2 cut(s) 150, 926
BsmFI GGGAC 1 cut(s) 527
BsnI GGCC 2 cut(s) 228, 605
Bsp1286I GDGCHC 4 cut(s) 249, 441, 749, 847
Bsp143I GATC 1 cut(s) 708
Bsp19I CCATGG 2 cut(s) 151, 750
BspACI CCGC 5 cut(s) 141, 375, 483, 488, 957
BspANI GGCC 2 cut(s) 228, 605
BspCNI CTCAG 2 cut(s) 77, 853
BspHI TCATGA 1 cut(s) 715
BspLI GGNNCC 3 cut(s) 481, 748, 855
BspMI ACCTGC 1 cut(s) 467
BspQI GCTCTTC 1 cut(s) 429
BspT107I GGYRCC 2 cut(s) 479, 746
BsrI ACTGG 1 cut(s) 848
BssECI CCNNGG 5 cut(s) 151, 209, 237, 741, 750
BssMI GATC 1 cut(s) 708
BssT1I CCWWGG 4 cut(s) 151, 237, 741, 750
Bst2UI CCWGG 1 cut(s) 210
Bst4CI ACNGT 4 cut(s) 205, 511, 643, 772
Bst6I CTCTTC 1 cut(s) 429
BstAPI GCANNNNNTGC 1 cut(s) 35
BstC8I GCNNGC 2 cut(s) 36, 809
BstDEI CTNAG 2 cut(s) 64, 861
BstDSI CCRYGG 2 cut(s) 151, 750
BstF5I GGATG 2 cut(s) 50, 760
BstKTI GATC 1 cut(s) 711
BstMAI GTCTC 2 cut(s) 150, 926
BstMBI GATC 1 cut(s) 708
BstMWI GCNNNNNNNGC 7 cut(s) 35, 147, 324, 482, 629, 862, 911
BstNI CCWGG 1 cut(s) 210
BstNSI RCATGY 1 cut(s) 38
BstSCI CCNGG 2 cut(s) 208, 550
BstSLI GKGCMC 1 cut(s) 749
BstV1I GCAGC 2 cut(s) 37, 724
BstV2I GAAGAC 1 cut(s) 865
Bsu36I CCTNAGG 1 cut(s) 64
BsuRI GGCC 2 cut(s) 228, 605
BtgI CCRYGG 2 cut(s) 151, 750
BtsCI GGATG 2 cut(s) 50, 760
BtsI GCAGTG 3 cut(s) 36, 684, 1011
BtsIMutI CAGTG 6 cut(s) 36, 639, 684, 768, 855, 1011
BveI ACCTGC 1 cut(s) 467
Cac8I GCNNGC 2 cut(s) 36, 809
CciI TCATGA 1 cut(s) 715
Cfr13I GGNCC 1 cut(s) 366
CviAII CATG 9 cut(s) 35, 152, 215, 295, 306, 315, 716, 722, 751
DdeI CTNAG 2 cut(s) 64, 861
DpnI GATC 1 cut(s) 710
DpnII GATC 1 cut(s) 708
EaeI YGGCCR 2 cut(s) 226, 603
Eam1104I CTCTTC 1 cut(s) 429
EarI CTCTTC 1 cut(s) 429
Eco130I CCWWGG 4 cut(s) 151, 237, 741, 750
Eco24I GRGCYC 1 cut(s) 249
Eco32I GATATC 1 cut(s) 118
Eco47I GGWCC 1 cut(s) 366
Eco57I CTGAAG 1 cut(s) 355
Eco81I CCTNAGG 1 cut(s) 64
EcoRI GAATTC 1 cut(s) 69
EcoRII CCWGG 1 cut(s) 208
EcoRV GATATC 1 cut(s) 118
EcoT14I CCWWGG 4 cut(s) 151, 237, 741, 750
EcoT38I GRGCYC 1 cut(s) 249
ErhI CCWWGG 4 cut(s) 151, 237, 741, 750
FaeI CATG 9 cut(s) 38, 155, 218, 298, 309, 318, 719, 725, 754
FaqI GGGAC 1 cut(s) 527
FatI CATG 9 cut(s) 34, 151, 214, 294, 305, 314, 715, 721, 750
FauI CCCGC 1 cut(s) 495
FauNDI CATATG 1 cut(s) 600
FbaI TGATCA 1 cut(s) 708
FblI GTMKAC 1 cut(s) 188
Fnu4HI GCNGC 4 cut(s) 51, 483, 738, 957
FokI GGATG 2 cut(s) 57, 767
FriOI GRGCYC 1 cut(s) 249
Fsp4HI GCNGC 4 cut(s) 51, 483, 738, 957
FspBI CTAG 7 cut(s) 54, 93, 473, 539, 819, 830, 1096
GluI GCNGC 4 cut(s) 51, 483, 738, 957
GsaI CCCAGC 2 cut(s) 504, 550
GsuI CTGGAG 1 cut(s) 831
HaeIII GGCC 2 cut(s) 228, 605
HapII CCGG 2 cut(s) 282, 552
Hin1II CATG 9 cut(s) 38, 155, 218, 298, 309, 318, 719, 725, 754
HincII GTYRAC 1 cut(s) 189
HindII GTYRAC 1 cut(s) 189
HindIII AAGCTT 1 cut(s) 621
HinfI GANTC 4 cut(s) 59, 453, 698, 934
HpaII CCGG 2 cut(s) 282, 552
HphI GGTGA 2 cut(s) 112, 155
Hpy166II GTNNAC 2 cut(s) 189, 1009
Hpy188I TCNGA 6 cut(s) 201, 418, 452, 536, 629, 660
Hpy188III TCNNGA 6 cut(s) 20, 66, 127, 716, 938, 1019
Hpy8I GTNNAC 2 cut(s) 189, 1009
HpyAV CCTTC 3 cut(s) 90, 379, 1027
HpyCH4III ACNGT 4 cut(s) 205, 511, 643, 772
HpyCH4IV ACGT 1 cut(s) 252
HpyCH4V TGCA 6 cut(s) 34, 294, 327, 695, 914, 1091
HpyF10VI GCNNNNNNNGC 7 cut(s) 35, 147, 324, 482, 629, 862, 911
HpyF3I CTNAG 2 cut(s) 64, 861
HpySE526I ACGT 1 cut(s) 252
Hsp92II CATG 9 cut(s) 38, 155, 218, 298, 309, 318, 719, 725, 754
Ksp22I TGATCA 1 cut(s) 708
Kzo9I GATC 1 cut(s) 708
LguI GCTCTTC 1 cut(s) 429
LmnI GCTCC 3 cut(s) 629, 850, 853
Lsp1109I GCAGC 2 cut(s) 37, 724
MaeI CTAG 7 cut(s) 54, 93, 473, 539, 819, 830, 1096
MaeII ACGT 1 cut(s) 252
MaeIII GTNAC 2 cut(s) 232, 637
MalI GATC 1 cut(s) 710
MboI GATC 1 cut(s) 708
MboII GAAGA 4 cut(s) 35, 398, 446, 865
MfeI CAATTG 1 cut(s) 915
MhlI GDGCHC 4 cut(s) 249, 441, 749, 847
MlsI TGGCCA 1 cut(s) 228
MluCI AATT 8 cut(s) 69, 144, 219, 260, 343, 728, 882, 915
MluNI TGGCCA 1 cut(s) 228
MlyI GAGTC 1 cut(s) 928
MmeI TCCRAC 2 cut(s) 224, 250
MnlI CCTC 7 cut(s) 73, 176, 581, 664, 727, 803, 885
Mox20I TGGCCA 1 cut(s) 228
MroXI GAANNNNTTC 3 cut(s) 73, 530, 990
MscI TGGCCA 1 cut(s) 228
MseI TTAA 2 cut(s) 881, 1047
MslI CAYNNNNRTG 2 cut(s) 39, 720
Msp20I TGGCCA 1 cut(s) 228
MspA1I CMGCKG 1 cut(s) 375
MspI CCGG 2 cut(s) 282, 552
MspR9I CCNGG 2 cut(s) 210, 552
MunI CAATTG 1 cut(s) 915
MvaI CCWGG 1 cut(s) 210
MwoI GCNNNNNNNGC 7 cut(s) 35, 147, 324, 482, 629, 862, 911
NciI CCSGG 1 cut(s) 552
NcoI CCATGG 2 cut(s) 151, 750
NdeI CATATG 1 cut(s) 600
NdeII GATC 1 cut(s) 708
NlaIII CATG 9 cut(s) 38, 155, 218, 298, 309, 318, 719, 725, 754
NlaIV GGNNCC 3 cut(s) 481, 748, 855
NmuCI GTSAC 2 cut(s) 232, 637
NspI RCATGY 1 cut(s) 38
PaeI GCATGC 1 cut(s) 38
PagI TCATGA 1 cut(s) 715
PaqCI CACCTGC 1 cut(s) 467
PciSI GCTCTTC 1 cut(s) 429
PdmI GAANNNNTTC 3 cut(s) 73, 530, 990
PfeI GAWTC 3 cut(s) 59, 453, 698
PflMI CCANNNNNTGG 1 cut(s) 803
PkrI GCNGC 4 cut(s) 52, 484, 739, 958
PleI GAGTC 1 cut(s) 928
PpsI GAGTC 1 cut(s) 928
Psp6I CCWGG 1 cut(s) 208
PspFI CCCAGC 2 cut(s) 500, 546
PspGI CCWGG 1 cut(s) 208
PspN4I GGNNCC 3 cut(s) 481, 748, 855
PspPI GGNCC 1 cut(s) 366
RseI CAYNNNNRTG 2 cut(s) 39, 720
SalI GTCGAC 1 cut(s) 187
SapI GCTCTTC 1 cut(s) 429
SaqAI TTAA 2 cut(s) 881, 1047
SatI GCNGC 4 cut(s) 51, 483, 738, 957
Sau3AI GATC 1 cut(s) 708
Sau96I GGNCC 1 cut(s) 366
SchI GAGTC 1 cut(s) 928
ScrFI CCNGG 2 cut(s) 210, 552
SduI GDGCHC 4 cut(s) 249, 441, 749, 847
SinI GGWCC 1 cut(s) 366
SmiMI CAYNNNNRTG 2 cut(s) 39, 720
SmlI CTYRAG 3 cut(s) 18, 936, 1028
SmoI CTYRAG 3 cut(s) 18, 936, 1028
SpeI ACTAGT 3 cut(s) 538, 818, 829
SphI GCATGC 1 cut(s) 38
Sse9I AATT 8 cut(s) 69, 144, 219, 260, 343, 728, 882, 915
SsiI CCGC 5 cut(s) 141, 375, 483, 488, 957
SspI AATATT 2 cut(s) 137, 530
SspMI CTAG 7 cut(s) 54, 93, 473, 539, 819, 830, 1096
StyD4I CCNGG 2 cut(s) 208, 550
StyI CCWWGG 4 cut(s) 151, 237, 741, 750
TaaI ACNGT 4 cut(s) 205, 511, 643, 772
TaiI ACGT 1 cut(s) 255
TaqI TCGA 2 cut(s) 188, 984
TasI AATT 8 cut(s) 69, 144, 219, 260, 343, 728, 882, 915
TauI GCSGC 2 cut(s) 485, 959
TfiI GAWTC 3 cut(s) 59, 453, 698
Tru1I TTAA 2 cut(s) 881, 1047
Tru9I TTAA 2 cut(s) 881, 1047
TscAI CASTG 6 cut(s) 36, 646, 684, 775, 855, 1018
TseFI GTSAC 2 cut(s) 232, 637
TseI GCWGC 2 cut(s) 50, 737
Tsp45I GTSAC 2 cut(s) 232, 637
TspDTI ATGAA 5 cut(s) 231, 294, 399, 690, 941
TspRI CASTG 6 cut(s) 36, 646, 684, 775, 855, 1018
Van91I CCANNNNNTGG 1 cut(s) 803
VpaK11BI GGWCC 1 cut(s) 366
XapI RAATTY 3 cut(s) 69, 144, 260
XceI RCATGY 1 cut(s) 38
XmiI GTMKAC 1 cut(s) 188
XmnI GAANNNNTTC 3 cut(s) 73, 530, 990
XspI CTAG 7 cut(s) 54, 93, 473, 539, 819, 830, 1096
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.