Rorug06G0054100

Dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0002881
Physical Location & Seq
Reverse (-)
7498158 .. 7500648
2491 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0054100.1

Sequence Viewer

Length: 1401 bp
ATGAATATAAGAAGACACTGCTTGCAAACAAAGAGCCAGACACAGTGCGATCATCACTCAGCTTTTCTGATCTGTGATCTCACTGGCGTTCCCAGCCTTGACACTCCTTGCAAAAGATGTCAGCATCCCAAGGAGAATTGGTTTTGTTTGTCCTGTAAGGACGTCCACTGTCTTGTTAACCATCATGATATTGAGCATTATCAGCAGACAAAGCATTGTCTTTGTGTCAACTGCAGTACCAAAAGAATTTGGTGTTTCTCCTGCCATAAATATCTAGATGCGGAAGTGATCCAACAATTGTGGGCCAATAAGCAGAGTCGATCACCTTCAGCCAAGCTCGAAAGCATTGATGCAAAGGTTGTTGATGTTTCTGATCTCTTGTCCAAGCCATTTCCTAGCATCAATGTTCATCCACAGTTGGATGCTCCGTTGTTTGTTGGCCAGGACGAGCTTGCCTTGGCTAAGGAGGGACTCCGGAATGCCTTTGATCGCGGGTTAAAAGCTCTAGCTGATCCCAAGGTGCAAGAAGAATTTCTCCTTTACTCAGCCACGCTGCTATCAGCCGAGTCATGCCCCTCAGAATTGAAGGTTAACCTTTTATCATTCAGATGTAATCTTTCGGAAGAAACCTCTGCCTTCATTAAAGCTCAAGATGAGTTGAAGGTGGCCTCAGAGTTATCAGCCTCGGTTGCCCAGAAGTTGTTTGTGGTGAGGCAACAAACTTCAAAGTATGACGAGGTAAAGAAAGAAATGATTGCCTCGGACGAGAAAGTTGCCAACTGTAAGTCCATGATCAAAGAGTTGGCGGCACAAATCAAAGGGTTGGAAGCTTGTTTAGCCACAGAAGTGAGCAACAGGAAAAAGATTGATGAGGTTATCGATTCGATCGAGACACAAGTCACCTTAGCGAGAGATAGACTGGTCCCGGACTTGGCACGAGTGTCTTCCATGAAGGGAACGACCCAAGCAGCTTACCAGTTAGTAACTCGAAAAAAGTCGCACTGGGATAACCTAAAGATTATGTTTACTAGGTTTGCAATTGCAGACGCCACCATGAGCCGACATCCTAGTGTGAAGTGGGCTCAGCAGCCTGATACGCTTTACATCACTATTGAGTTGCCTGATGCCCAGGATGTAAAGCTCAAACTGGAGCCTGAAGGAAAGTTTCTATTCTCTGCTACAACTGGAGCAGAAAAGACACCTTATGTAGTTGATCTTGATCTCTATGACAAGATTGATGTAAATGAGAGTAGGTATAGTGTTGGCTCGAGAAATATCTGTTACCTAGTGAAAAAAGCGGAAAATAAATGGTGGAGCAGATTGATAAAACAAGAAAGAAAAGCTCCTGAGTTCTTGAAAGTTGATTGGGATAATTGGGGAGATGAGCAAGTCCATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
ADH_N ADH_zinc_N

Protein Analysis

466

Amino Acids

53.04

Weight (kDa)

6.91

Isoelectric Point (pI)

41.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-UBP PF02148 37 - 94 7.2e-12 Zn-finger in ubiquitin-hydrolases and other protein
CS PF04969 356 - 432 1.8e-11 CS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 165
AccII CGCG 1 cut(s) 492
AccIII TCCGGA 1 cut(s) 474
AciI CCGC 4 cut(s) 281, 492, 806, 1298
AclWI GGATC 2 cut(s) 283, 506
AcoI YGGCCR 1 cut(s) 439
AcsI RAATTY 2 cut(s) 246, 530
AcuI CTGAAG 2 cut(s) 312, 1176
AcyI GRCGYC 2 cut(s) 162, 1047
AfaI GTAC 1 cut(s) 238
AfiI CCNNNNNNNGG 1 cut(s) 931
AgsI TTSAA 4 cut(s) 586, 661, 726, 1357
AjnI CCWGG 2 cut(s) 441, 1128
AleI CACNNNNGTG 1 cut(s) 845
Alw26I GTCTC 1 cut(s) 884
AlwI GGATC 2 cut(s) 283, 506
Ama87I CYCGRG 1 cut(s) 1267
Aor13HI TCCGGA 1 cut(s) 474
AoxI GGCC 3 cut(s) 303, 439, 666
ApeKI GCWGC 3 cut(s) 553, 968, 1087
ApoI RAATTY 2 cut(s) 246, 530
Asp700I GAANNNNTTC 1 cut(s) 531
AspS9I GGNCC 2 cut(s) 303, 922
AsuC2I CCSGG 1 cut(s) 926
AsuHPI GGTGA 3 cut(s) 315, 721, 892
AvaI CYCGRG 1 cut(s) 1267
AvaII GGWCC 1 cut(s) 922
BaeI ACNNNNGTAYC 2 cut(s) 220, 253
BalI TGGCCA 1 cut(s) 441
BanII GRGCYC 1 cut(s) 1084
BauI CACGAG 1 cut(s) 936
BbsI GAAGAC 2 cut(s) 19, 936
BbvI GCAGC 3 cut(s) 540, 980, 1099
BccI CCATC 1 cut(s) 189
BcgI CGANNNNNNTGC 2 cut(s) 755, 789
BciT130I CCWGG 2 cut(s) 443, 1130
BclI TGATCA 1 cut(s) 792
BcnI CCSGG 1 cut(s) 926
BcoDI GTCTC 1 cut(s) 884
BfaI CTAG 6 cut(s) 275, 396, 506, 1029, 1068, 1286
BfmI CTRYAG 1 cut(s) 232
BisI GCNGC 4 cut(s) 554, 807, 969, 1088
BlpI GCTNAGC 1 cut(s) 1083
BlsI GCNGC 4 cut(s) 555, 808, 970, 1089
Bme1390I CCNGG 3 cut(s) 443, 926, 1130
Bme18I GGWCC 1 cut(s) 922
BmeT110I CYCGRG 1 cut(s) 1267
BmgT120I GGNCC 2 cut(s) 303, 922
BmiI GGNNCC 2 cut(s) 924, 1152
BmrFI CCNGG 3 cut(s) 443, 926, 1130
BmrI ACTGGG 1 cut(s) 1012
BmsI GCATC 6 cut(s) 133, 268, 340, 408, 412, 1114
BmuI ACTGGG 1 cut(s) 1012
BoxI GACNNNNGTC 1 cut(s) 896
BpiI GAAGAC 2 cut(s) 19, 936
BpmI CTGGAG 2 cut(s) 1169, 1206
Bpu10I CCTNAGC 2 cut(s) 462, 904
Bpu1102I GCTNAGC 1 cut(s) 1083
BpuEI CTTGAG 1 cut(s) 633
BpuMI CCSGG 1 cut(s) 926
Bsa29I ATCGAT 1 cut(s) 879
BsaBI GATNNNNATC 1 cut(s) 1218
BsaHI GRCGYC 2 cut(s) 162, 1047
BsaJI CCNNGG 6 cut(s) 129, 456, 516, 684, 759, 1128
BsaWI WCCGGW 1 cut(s) 474
BsaXI ACNNNNNCTCC 2 cut(s) 125, 155
Bsc4I CCNNNNNNNGG 1 cut(s) 931
Bse1I ACTGG 6 cut(s) 88, 924, 976, 1007, 1152, 1189
Bse8I GATNNNNATC 1 cut(s) 1218
BseAI TCCGGA 1 cut(s) 474
BseBI CCWGG 2 cut(s) 443, 1130
BseCI ATCGAT 1 cut(s) 879
BseDI CCNNGG 6 cut(s) 129, 456, 516, 684, 759, 1128
BseGI GGATG 5 cut(s) 124, 409, 427, 1063, 1138
BseJI GATNNNNATC 1 cut(s) 1218
BseLI CCNNNNNNNGG 1 cut(s) 931
BseMII CTCAG 6 cut(s) 72, 558, 591, 684, 1097, 1338
BseNI ACTGG 6 cut(s) 88, 924, 976, 1007, 1152, 1189
BseXI GCAGC 3 cut(s) 540, 980, 1099
BseYI CCCAGC 1 cut(s) 92
Bsh1236I CGCG 1 cut(s) 492
Bsh1285I CGRYCG 1 cut(s) 888
BshFI GGCC 3 cut(s) 305, 441, 668
BshVI ATCGAT 1 cut(s) 879
BsiEI CGRYCG 1 cut(s) 888
BsiHKCI CYCGRG 1 cut(s) 1267
BsiSI CCGG 2 cut(s) 475, 926
BslFI GGGAC 2 cut(s) 483, 908
BslI CCNNNNNNNGG 1 cut(s) 931
BsmAI GTCTC 1 cut(s) 884
BsmFI GGGAC 2 cut(s) 483, 908
BsmI GAATGC 1 cut(s) 484
BsnI GGCC 3 cut(s) 305, 441, 668
BsoBI CYCGRG 1 cut(s) 1267
Bsp1286I GDGCHC 1 cut(s) 1084
Bsp13I TCCGGA 1 cut(s) 474
Bsp1720I GCTNAGC 1 cut(s) 1083
BspACI CCGC 4 cut(s) 281, 492, 806, 1298
BspANI GGCC 3 cut(s) 305, 441, 668
BspCNI CTCAG 6 cut(s) 71, 557, 590, 683, 1096, 1339
BspDI ATCGAT 1 cut(s) 879
BspEI TCCGGA 1 cut(s) 474
BspFNI CGCG 1 cut(s) 492
BspHI TCATGA 1 cut(s) 184
BspLI GGNNCC 2 cut(s) 924, 1152
BspMAI CTGCAG 1 cut(s) 236
BspPI GGATC 2 cut(s) 283, 506
BsrI ACTGG 6 cut(s) 88, 924, 976, 1007, 1152, 1189
BssECI CCNNGG 6 cut(s) 129, 456, 516, 684, 759, 1128
BssNI GRCGYC 2 cut(s) 162, 1047
BssSI CACGAG 1 cut(s) 936
BssT1I CCWWGG 3 cut(s) 129, 456, 516
Bst2BI CACGAG 1 cut(s) 936
Bst2UI CCWGG 2 cut(s) 443, 1130
Bst4CI ACNGT 4 cut(s) 45, 170, 417, 782
BstACI GRCGYC 2 cut(s) 162, 1047
BstC8I GCNNGC 2 cut(s) 23, 453
BstDEI CTNAG 8 cut(s) 58, 462, 544, 577, 670, 904, 1083, 1347
BstF5I GGATG 5 cut(s) 124, 409, 427, 1063, 1138
BstFNI CGCG 1 cut(s) 492
BstMAI GTCTC 1 cut(s) 884
BstMCI CGRYCG 1 cut(s) 888
BstMWI GCNNNNNNNGC 6 cut(s) 93, 202, 211, 689, 836, 1096
BstNI CCWGG 2 cut(s) 443, 1130
BstPAI GACNNNNGTC 1 cut(s) 896
BstSCI CCNGG 3 cut(s) 441, 924, 1128
BstSFI CTRYAG 1 cut(s) 232
BstUI CGCG 1 cut(s) 492
BstV1I GCAGC 3 cut(s) 540, 980, 1099
BstV2I GAAGAC 2 cut(s) 19, 936
Bsu15I ATCGAT 1 cut(s) 879
BsuRI GGCC 3 cut(s) 305, 441, 668
BsuTUI ATCGAT 1 cut(s) 879
BtsCI GGATG 5 cut(s) 124, 409, 427, 1063, 1138
BtsI GCAGTG 1 cut(s) 16
BtsIMutI CAGTG 5 cut(s) 16, 50, 81, 166, 1000
Cac8I GCNNGC 2 cut(s) 23, 453
CciI TCATGA 1 cut(s) 184
Cfr13I GGNCC 2 cut(s) 303, 922
ClaI ATCGAT 1 cut(s) 879
CseI GACGC 1 cut(s) 1055
Csp6I GTAC 1 cut(s) 237
CspCI CAANNNNNGTGG 2 cut(s) 281, 316
CviAII CATG 6 cut(s) 185, 570, 790, 949, 1054, 1394
CviQI GTAC 1 cut(s) 237
DdeI CTNAG 8 cut(s) 58, 462, 544, 577, 670, 904, 1083, 1347
EaeI YGGCCR 1 cut(s) 439
Eco130I CCWWGG 3 cut(s) 129, 456, 516
Eco24I GRGCYC 1 cut(s) 1084
Eco47I GGWCC 1 cut(s) 922
Eco57I CTGAAG 2 cut(s) 312, 1176
Eco88I CYCGRG 1 cut(s) 1267
EcoRII CCWGG 2 cut(s) 441, 1128
EcoT14I CCWWGG 3 cut(s) 129, 456, 516
EcoT38I GRGCYC 1 cut(s) 1084
ErhI CCWWGG 3 cut(s) 129, 456, 516
FaeI CATG 6 cut(s) 188, 573, 793, 952, 1057, 1397
FaqI GGGAC 2 cut(s) 483, 908
FatI CATG 6 cut(s) 184, 569, 789, 948, 1053, 1393
FauI CCCGC 1 cut(s) 485
FbaI TGATCA 1 cut(s) 792
Fnu4HI GCNGC 4 cut(s) 554, 807, 969, 1088
FokI GGATG 5 cut(s) 111, 396, 434, 1050, 1145
FriOI GRGCYC 1 cut(s) 1084
Fsp4HI GCNGC 4 cut(s) 554, 807, 969, 1088
FspBI CTAG 6 cut(s) 275, 396, 506, 1029, 1068, 1286
GluI GCNGC 4 cut(s) 554, 807, 969, 1088
GsaI CCCAGC 1 cut(s) 96
GsuI CTGGAG 2 cut(s) 1169, 1206
HaeIII GGCC 3 cut(s) 305, 441, 668
HapII CCGG 2 cut(s) 475, 926
HgaI GACGC 1 cut(s) 1055
Hin1I GRCGYC 2 cut(s) 162, 1047
Hin1II CATG 6 cut(s) 188, 573, 793, 952, 1057, 1397
HincII GTYRAC 3 cut(s) 178, 229, 592
HindII GTYRAC 3 cut(s) 178, 229, 592
HindIII AAGCTT 1 cut(s) 828
HinfI GANTC 4 cut(s) 316, 471, 566, 881
HpaI GTTAAC 2 cut(s) 178, 592
HpaII CCGG 2 cut(s) 475, 926
HphI GGTGA 3 cut(s) 315, 721, 892
Hpy166II GTNNAC 5 cut(s) 166, 178, 229, 592, 1026
Hpy188I TCNGA 7 cut(s) 69, 373, 580, 608, 622, 673, 763
Hpy188III TCNNGA 9 cut(s) 185, 275, 475, 650, 889, 1217, 1269, 1346, 1354
Hpy8I GTNNAC 5 cut(s) 166, 178, 229, 592, 1026
HpyAV CCTTC 6 cut(s) 336, 580, 646, 655, 946, 1151
HpyCH4III ACNGT 4 cut(s) 45, 170, 417, 782
HpyCH4IV ACGT 1 cut(s) 162
HpyCH4V TGCA 7 cut(s) 25, 111, 234, 353, 523, 1037, 1043
HpyF10VI GCNNNNNNNGC 6 cut(s) 93, 202, 211, 689, 836, 1096
HpyF3I CTNAG 8 cut(s) 58, 462, 544, 577, 670, 904, 1083, 1347
HpySE526I ACGT 1 cut(s) 162
Hsp92I GRCGYC 2 cut(s) 162, 1047
Hsp92II CATG 6 cut(s) 188, 573, 793, 952, 1057, 1397
Kpn2I TCCGGA 1 cut(s) 474
Ksp22I TGATCA 1 cut(s) 792
KspAI GTTAAC 2 cut(s) 178, 592
LmnI GCTCC 5 cut(s) 430, 1150, 1187, 1314, 1348
Lsp1109I GCAGC 3 cut(s) 540, 980, 1099
LweI GCATC 6 cut(s) 133, 268, 340, 408, 412, 1114
MaeI CTAG 6 cut(s) 275, 396, 506, 1029, 1068, 1286
MaeII ACGT 1 cut(s) 162
MaeIII GTNAC 3 cut(s) 898, 982, 1280
MboII GAAGA 4 cut(s) 24, 539, 635, 936
MfeI CAATTG 2 cut(s) 296, 1038
MhlI GDGCHC 1 cut(s) 1084
MlsI TGGCCA 1 cut(s) 441
MluCI AATT 7 cut(s) 136, 246, 296, 530, 581, 1038, 1372
MluNI TGGCCA 1 cut(s) 441
MlyI GAGTC 3 cut(s) 325, 465, 575
MmeI TCCRAC 3 cut(s) 316, 399, 804
MnlI CCTC 9 cut(s) 460, 586, 640, 679, 694, 705, 730, 769, 865
Mox20I TGGCCA 1 cut(s) 441
MroI TCCGGA 1 cut(s) 474
MroXI GAANNNNTTC 1 cut(s) 531
MscI TGGCCA 1 cut(s) 441
MseI TTAA 4 cut(s) 177, 497, 591, 642
MslI CAYNNNNRTG 3 cut(s) 607, 845, 1068
Msp20I TGGCCA 1 cut(s) 441
MspI CCGG 2 cut(s) 475, 926
MspR9I CCNGG 3 cut(s) 443, 926, 1130
MunI CAATTG 2 cut(s) 296, 1038
Mva1269I GAATGC 1 cut(s) 484
MvaI CCWGG 2 cut(s) 443, 1130
MvnI CGCG 1 cut(s) 492
MwoI GCNNNNNNNGC 6 cut(s) 93, 202, 211, 689, 836, 1096
NciI CCSGG 1 cut(s) 926
NlaIII CATG 6 cut(s) 188, 573, 793, 952, 1057, 1397
NlaIV GGNNCC 2 cut(s) 924, 1152
NmeAIII GCCGAG 1 cut(s) 589
NmuCI GTSAC 1 cut(s) 898
OliI CACNNNNGTG 1 cut(s) 845
PaeR7I CTCGAG 1 cut(s) 1267
PagI TCATGA 1 cut(s) 184
PcsI WCGNNNNNNNCGW 1 cut(s) 885
PctI GAATGC 1 cut(s) 484
PdmI GAANNNNTTC 1 cut(s) 531
PfeI GAWTC 1 cut(s) 881
PfoI TCCNGGA 1 cut(s) 924
PkrI GCNGC 4 cut(s) 555, 808, 970, 1089
Ple19I CGATCG 1 cut(s) 888
PleI GAGTC 3 cut(s) 324, 465, 574
PpsI GAGTC 3 cut(s) 324, 465, 574
PshAI GACNNNNGTC 1 cut(s) 896
Psp6I CCWGG 2 cut(s) 441, 1128
PspFI CCCAGC 1 cut(s) 92
PspGI CCWGG 2 cut(s) 441, 1128
PspN4I GGNNCC 2 cut(s) 924, 1152
PspPI GGNCC 2 cut(s) 303, 922
PstI CTGCAG 1 cut(s) 236
PvuI CGATCG 1 cut(s) 888
RsaI GTAC 1 cut(s) 238
RsaNI GTAC 1 cut(s) 237
RseI CAYNNNNRTG 3 cut(s) 607, 845, 1068
SaqAI TTAA 4 cut(s) 177, 497, 591, 642
SatI GCNGC 4 cut(s) 554, 807, 969, 1088
Sau96I GGNCC 2 cut(s) 303, 922
SchI GAGTC 3 cut(s) 325, 465, 575
ScrFI CCNGG 3 cut(s) 443, 926, 1130
SduI GDGCHC 1 cut(s) 1084
SfaNI GCATC 6 cut(s) 133, 268, 340, 408, 412, 1114
SfcI CTRYAG 1 cut(s) 232
Sfr274I CTCGAG 1 cut(s) 1267
SinI GGWCC 1 cut(s) 922
SlaI CTCGAG 1 cut(s) 1267
SmiMI CAYNNNNRTG 3 cut(s) 607, 845, 1068
SmlI CTYRAG 2 cut(s) 648, 1267
SmoI CTYRAG 2 cut(s) 648, 1267
Sse9I AATT 7 cut(s) 136, 246, 296, 530, 581, 1038, 1372
SsiI CCGC 4 cut(s) 281, 492, 806, 1298
SspMI CTAG 6 cut(s) 275, 396, 506, 1029, 1068, 1286
StyD4I CCNGG 3 cut(s) 441, 924, 1128
StyI CCWWGG 3 cut(s) 129, 456, 516
TaaI ACNGT 4 cut(s) 45, 170, 417, 782
TaiI ACGT 1 cut(s) 165
TaqI TCGA 7 cut(s) 319, 339, 879, 884, 888, 988, 1268
TasI AATT 7 cut(s) 136, 246, 296, 530, 581, 1038, 1372
TauI GCSGC 1 cut(s) 809
TfiI GAWTC 1 cut(s) 881
Tru1I TTAA 4 cut(s) 177, 497, 591, 642
Tru9I TTAA 4 cut(s) 177, 497, 591, 642
TscAI CASTG 5 cut(s) 23, 50, 88, 173, 1007
TseFI GTSAC 1 cut(s) 898
TseI GCWGC 3 cut(s) 553, 968, 1087
Tsp45I GTSAC 1 cut(s) 898
TspDTI ATGAA 4 cut(s) 17, 398, 628, 965
TspGWI ACGGA 1 cut(s) 417
TspRI CASTG 5 cut(s) 23, 50, 88, 173, 1007
VpaK11BI GGWCC 1 cut(s) 922
XapI RAATTY 2 cut(s) 246, 530
XbaI TCTAGA 1 cut(s) 274
XhoI CTCGAG 1 cut(s) 1267
XmnI GAANNNNTTC 1 cut(s) 531
XspI CTAG 6 cut(s) 275, 396, 506, 1029, 1068, 1286
ZraI GACGTC 1 cut(s) 163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.