FvH4_4g16540

ER lumen

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001878
Physical Location & Seq
Forward (+)
20449174 .. 20454991
5818 bp
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UTR
Exon/CDS
Intron
FvH4_4g16540.t1

Sequence Viewer

Length: 648 bp
ATGAATATATTCAGATTAGCGGGAGATATGACGCATTTGGCTAGTGTTCTTGTTTTGCTTCTCAAGATCCACACTATCAAGTCCTGTGCTGGTGTTTCCTTGAAGACTCAAGAACTCTATGCTCTTGTCTTTGCTACCCGCTACTTGGATATCTTTGTTAGCTTCTACTCAGTTTATAATACCCTCATGAAGTTGATATTCTTGGGTAGCTCTTTCTCAATTGTTTGGTATATACGGCACCACAGGATTGTTCGTAGATCGTATGATAAGTCTCAAGACACATTTCGCCATCATTTTCTTCTACTCCCTTGCCTGCTTTTGGCCCTGGTCATCAATGAGAAATTCACTTTTAGAGAGGTGATGTGGGCCTTTTCCATATATTTGGAAGCAGTCGCCATACTTCCTCAGCTGGTGCTGTTGCAAAGGACAAGAAATATTGACAACTTAACAGGCCAATATGTAGTCCTCCTCGGGGCATATCGAGCATTGTACATATTGAATTGGATTTACCGCTACTTTACTGAGGAAGGCTTTGTCCACTGGATAACTTGGATGGCAGGGATAATCCAGACCTTACTCTATGCTGATTTCTTCTATTATTACTTCCAGAGTTGGAAGAACAATGTAAAGCTCCAGTTGCCAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05110 map05110
Pfam Domains
Protein Families
ER_lumen_recept

Protein Analysis

216

Amino Acids

25.53

Weight (kDa)

9.47

Isoelectric Point (pI)

38.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ER_lumen_recept PF00810 28 - 171 3e-58 ER lumen protein retaining receptor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 177
AccB1I GGYRCC 1 cut(s) 237
AciI CCGC 3 cut(s) 20, 139, 511
AclWI GGATC 1 cut(s) 61
AcsI RAATTY 1 cut(s) 341
AfaI GTAC 1 cut(s) 491
AfiI CCNNNNNNNGG 3 cut(s) 145, 319, 472
AgsI TTSAA 2 cut(s) 103, 499
AjnI CCWGG 1 cut(s) 324
AluBI AGCT 5 cut(s) 162, 210, 409, 631, 644
AluI AGCT 5 cut(s) 162, 210, 409, 631, 644
Alw26I GTCTC 1 cut(s) 276
AlwI GGATC 1 cut(s) 61
Ama87I CYCGRG 1 cut(s) 470
AoxI GGCC 3 cut(s) 321, 366, 451
ApoI RAATTY 1 cut(s) 341
Asp700I GAANNNNTTC 1 cut(s) 8
AspS9I GGNCC 2 cut(s) 322, 366
AsuHPI GGTGA 1 cut(s) 370
AvaI CYCGRG 1 cut(s) 470
BanI GGYRCC 1 cut(s) 237
BbsI GAAGAC 1 cut(s) 110
BbvCI CCTCAGC 1 cut(s) 405
BccI CCATC 2 cut(s) 297, 547
BceAI ACGGC 1 cut(s) 251
BciT130I CCWGG 1 cut(s) 326
BcoDI GTCTC 1 cut(s) 276
BfaI CTAG 1 cut(s) 42
Bme1390I CCNGG 1 cut(s) 326
BmeT110I CYCGRG 1 cut(s) 470
BmgT120I GGNCC 2 cut(s) 322, 366
BmiI GGNNCC 1 cut(s) 239
BmrFI CCNGG 1 cut(s) 326
BpiI GAAGAC 1 cut(s) 110
BpmI CTGGAG 1 cut(s) 617
Bpu10I CCTNAGC 1 cut(s) 405
BpuEI CTTGAG 3 cut(s) 47, 93, 258
BsaJI CCNNGG 2 cut(s) 324, 469
Bsc4I CCNNNNNNNGG 3 cut(s) 145, 319, 472
Bse1I ACTGG 2 cut(s) 545, 634
BseBI CCWGG 1 cut(s) 326
BseDI CCNNGG 2 cut(s) 324, 469
BseGI GGATG 1 cut(s) 558
BseLI CCNNNNNNNGG 3 cut(s) 145, 319, 472
BseMII CTCAG 3 cut(s) 183, 419, 513
BseNI ACTGG 2 cut(s) 545, 634
BseRI GAGGAG 1 cut(s) 458
BshFI GGCC 3 cut(s) 323, 368, 453
BshNI GGYRCC 1 cut(s) 237
BsiHKCI CYCGRG 1 cut(s) 470
BslI CCNNNNNNNGG 3 cut(s) 145, 319, 472
BsmAI GTCTC 1 cut(s) 276
BsnI GGCC 3 cut(s) 323, 368, 453
BsoBI CYCGRG 1 cut(s) 470
Bsp1407I TGTACA 1 cut(s) 489
Bsp143I GATC 2 cut(s) 66, 257
BspACI CCGC 3 cut(s) 20, 139, 511
BspANI GGCC 3 cut(s) 323, 368, 453
BspCNI CTCAG 3 cut(s) 182, 418, 514
BspHI TCATGA 1 cut(s) 186
BspLI GGNNCC 1 cut(s) 239
BspPI GGATC 1 cut(s) 61
BspT107I GGYRCC 1 cut(s) 237
BsrGI TGTACA 1 cut(s) 489
BsrI ACTGG 2 cut(s) 545, 634
BssECI CCNNGG 2 cut(s) 324, 469
BssMI GATC 2 cut(s) 66, 257
Bst2UI CCWGG 1 cut(s) 326
BstAUI TGTACA 1 cut(s) 489
BstC8I GCNNGC 2 cut(s) 314, 642
BstDEI CTNAG 3 cut(s) 169, 405, 522
BstF5I GGATG 1 cut(s) 558
BstKTI GATC 2 cut(s) 69, 260
BstMAI GTCTC 1 cut(s) 276
BstMBI GATC 2 cut(s) 66, 257
BstMWI GCNNNNNNNGC 2 cut(s) 482, 637
BstNI CCWGG 1 cut(s) 326
BstSCI CCNGG 1 cut(s) 324
BstV2I GAAGAC 1 cut(s) 110
BstX2I RGATCY 1 cut(s) 66
BstXI CCANNNNNNTGG 1 cut(s) 382
BstYI RGATCY 1 cut(s) 66
BsuRI GGCC 3 cut(s) 323, 368, 453
BtsCI GGATG 1 cut(s) 558
BtsIMutI CAGTG 1 cut(s) 538
Cac8I GCNNGC 2 cut(s) 314, 642
CciI TCATGA 1 cut(s) 186
Cfr13I GGNCC 2 cut(s) 322, 366
CseI GACGC 1 cut(s) 40
Csp6I GTAC 1 cut(s) 490
CviAII CATG 1 cut(s) 187
CviQI GTAC 1 cut(s) 490
DdeI CTNAG 3 cut(s) 169, 405, 522
DpnI GATC 2 cut(s) 68, 259
DpnII GATC 2 cut(s) 66, 257
Eco32I GATATC 1 cut(s) 151
Eco88I CYCGRG 1 cut(s) 470
EcoRII CCWGG 1 cut(s) 324
EcoRV GATATC 1 cut(s) 151
FaeI CATG 1 cut(s) 190
FatI CATG 1 cut(s) 186
FauI CCCGC 2 cut(s) 13, 146
FokI GGATG 1 cut(s) 565
FspBI CTAG 1 cut(s) 42
GsuI CTGGAG 1 cut(s) 617
HaeIII GGCC 3 cut(s) 323, 368, 453
HgaI GACGC 1 cut(s) 40
Hin1II CATG 1 cut(s) 190
HinfI GANTC 1 cut(s) 106
HphI GGTGA 1 cut(s) 370
Hpy166II GTNNAC 1 cut(s) 538
Hpy188I TCNGA 1 cut(s) 14
Hpy188III TCNNGA 6 cut(s) 64, 110, 187, 275, 568, 607
Hpy8I GTNNAC 1 cut(s) 538
HpyAV CCTTC 1 cut(s) 521
HpyCH4V TGCA 1 cut(s) 421
HpyF10VI GCNNNNNNNGC 2 cut(s) 482, 637
HpyF3I CTNAG 3 cut(s) 169, 405, 522
Hsp92II CATG 1 cut(s) 190
Kzo9I GATC 2 cut(s) 66, 257
LmnI GCTCC 1 cut(s) 636
MaeI CTAG 1 cut(s) 42
MalI GATC 2 cut(s) 68, 259
MboI GATC 2 cut(s) 66, 257
MboII GAAGA 4 cut(s) 115, 290, 583, 628
MfeI CAATTG 1 cut(s) 219
MflI RGATCY 1 cut(s) 66
MluCI AATT 3 cut(s) 219, 341, 499
MlyI GAGTC 1 cut(s) 100
MmeI TCCRAC 1 cut(s) 593
MnlI CCTC 6 cut(s) 194, 349, 414, 476, 479, 517
MroXI GAANNNNTTC 1 cut(s) 8
MseI TTAA 1 cut(s) 446
MspA1I CMGCKG 1 cut(s) 409
MspR9I CCNGG 1 cut(s) 326
MunI CAATTG 1 cut(s) 219
MvaI CCWGG 1 cut(s) 326
MwoI GCNNNNNNNGC 2 cut(s) 482, 637
NdeII GATC 2 cut(s) 66, 257
NlaIII CATG 1 cut(s) 190
NlaIV GGNNCC 1 cut(s) 239
PagI TCATGA 1 cut(s) 186
PdmI GAANNNNTTC 1 cut(s) 8
PleI GAGTC 1 cut(s) 100
PpsI GAGTC 1 cut(s) 100
PsiI TTATAA 1 cut(s) 177
Psp6I CCWGG 1 cut(s) 324
PspGI CCWGG 1 cut(s) 324
PspN4I GGNNCC 1 cut(s) 239
PspPI GGNCC 2 cut(s) 322, 366
PsuI RGATCY 1 cut(s) 66
PvuII CAGCTG 1 cut(s) 409
RsaI GTAC 1 cut(s) 491
RsaNI GTAC 1 cut(s) 490
SaqAI TTAA 1 cut(s) 446
Sau3AI GATC 2 cut(s) 66, 257
Sau96I GGNCC 2 cut(s) 322, 366
SchI GAGTC 1 cut(s) 100
ScrFI CCNGG 1 cut(s) 326
SetI ASST 7 cut(s) 164, 212, 360, 411, 575, 633, 646
SmlI CTYRAG 3 cut(s) 62, 108, 273
SmoI CTYRAG 3 cut(s) 62, 108, 273
Sse9I AATT 3 cut(s) 219, 341, 499
SsiI CCGC 3 cut(s) 20, 139, 511
SspI AATATT 1 cut(s) 436
SspMI CTAG 1 cut(s) 42
StyD4I CCNGG 1 cut(s) 324
TaqI TCGA 1 cut(s) 481
TasI AATT 3 cut(s) 219, 341, 499
TatI WGTACW 1 cut(s) 489
Tru1I TTAA 1 cut(s) 446
Tru9I TTAA 1 cut(s) 446
TscAI CASTG 1 cut(s) 545
TspDTI ATGAA 2 cut(s) 17, 203
TspRI CASTG 1 cut(s) 545
XapI RAATTY 1 cut(s) 341
XmnI GAANNNNTTC 1 cut(s) 8
XspI CTAG 1 cut(s) 42
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.