pycom07g28010

ER lumen

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0001878
Physical Location & Seq
Forward (+)
27520910 .. 27522919
2010 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g28010.5

Sequence Viewer

Length: 648 bp
ATGAATCTGTTTAGGTTGGCTGGAGACATAACCCACTTGTTAAGCATATTGGTCCTGTTCCTCAAGATCCGCTCCACCAAATCTTGTGCAGGAATTTCACTCAAAACTCAAGAACTCTATGCGTTGGTCTTTCTTACTCGATACCTTGACTTGATCGTCAAGTATTACTCAGTGTACAACACTCTGGGGAAGCTCATCTTTATCGGAACTTCTTTTGCCACGGTCTGGCACATGAGGTACCATAAAGTAGTGAAGCAAACCTACAACAAGGATCAAGATACTTTCAGACATTACTATCTCATACTTTTATCCTTGGGGCTGGCTCTTCTGATTCCGCGTGCTTTTACTGTAATTGAGGTTCTGTGGGCATTTTCAATATATCTTGAAGCTGTGGCAATCTTACCACAGTTGGTATTACTGCAGAGGTCAAGAAACATTGACAACTTAACTGGAAATTATGTTTTCCTTCTCGGTGCTTATCGTTCTTTATATCTTGTCAACTGGATTTATCGTTTCTTCACAGAACTCCACAGAGTTCGCTGGATACCTTGGATTTCAGGCCTTGTTCAGACTGCTCTTTATGCTGATTTTTTCTACTACTATATCAAGAGCTGGAAGGAACACGAGGAGCTTAAGCTTCCCGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05110 map05110
Pfam Domains
Protein Families
ER_lumen_recept

Protein Analysis

216

Amino Acids

25.46

Weight (kDa)

9.59

Isoelectric Point (pI)

39.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 155
Acc65I GGTACC 1 cut(s) 237
AccB1I GGYRCC 1 cut(s) 237
AccB7I CCANNNNNTGG 1 cut(s) 225
AccBSI CCGCTC 1 cut(s) 72
AccII CGCG 1 cut(s) 337
AciI CCGC 3 cut(s) 70, 335, 642
AclWI GGATC 2 cut(s) 61, 279
AcsI RAATTY 1 cut(s) 93
AfaI GTAC 2 cut(s) 176, 239
AfiI CCNNNNNNNGG 1 cut(s) 225
AflII CTTAAG 1 cut(s) 632
AgsI TTSAA 2 cut(s) 375, 386
AluBI AGCT 5 cut(s) 193, 389, 612, 631, 637
AluI AGCT 5 cut(s) 193, 389, 612, 631, 637
Alw26I GTCTC 1 cut(s) 18
AlwI GGATC 2 cut(s) 61, 279
AoxI GGCC 1 cut(s) 559
ApoI RAATTY 1 cut(s) 93
Asp718I GGTACC 1 cut(s) 237
AspS9I GGNCC 1 cut(s) 52
AvaII GGWCC 1 cut(s) 52
BanI GGYRCC 1 cut(s) 237
BarI GAAGNNNNNNTAC 2 cut(s) 245, 277
BauI CACGAG 1 cut(s) 623
BciVI GTATCC 1 cut(s) 537
BcoDI GTCTC 1 cut(s) 18
BfmI CTRYAG 1 cut(s) 419
BfrI CTTAAG 1 cut(s) 632
BfuI GTATCC 1 cut(s) 537
Bme18I GGWCC 1 cut(s) 52
BmgT120I GGNCC 1 cut(s) 52
BmiI GGNNCC 1 cut(s) 239
BpmI CTGGAG 1 cut(s) 42
BpuEI CTTGAG 2 cut(s) 47, 93
BsaJI CCNNGG 3 cut(s) 219, 312, 548
Bsc4I CCNNNNNNNGG 1 cut(s) 225
Bse1I ACTGG 2 cut(s) 454, 506
BseDI CCNNGG 3 cut(s) 219, 312, 548
BseLI CCNNNNNNNGG 1 cut(s) 225
BseMII CTCAG 1 cut(s) 183
BseNI ACTGG 2 cut(s) 454, 506
BseRI GAGGAG 1 cut(s) 641
BsgI GTGCAG 1 cut(s) 108
Bsh1236I CGCG 1 cut(s) 337
BshFI GGCC 1 cut(s) 561
BshNI GGYRCC 1 cut(s) 237
BslI CCNNNNNNNGG 1 cut(s) 225
BsmAI GTCTC 1 cut(s) 18
BsnI GGCC 1 cut(s) 561
Bsp1407I TGTACA 1 cut(s) 174
Bsp143I GATC 3 cut(s) 66, 153, 271
BspACI CCGC 3 cut(s) 70, 335, 642
BspANI GGCC 1 cut(s) 561
BspCNI CTCAG 1 cut(s) 182
BspFNI CGCG 1 cut(s) 337
BspLI GGNNCC 1 cut(s) 239
BspMAI CTGCAG 1 cut(s) 423
BspPI GGATC 2 cut(s) 61, 279
BspQI GCTCTTC 1 cut(s) 330
BspT107I GGYRCC 1 cut(s) 237
BspTI CTTAAG 1 cut(s) 632
BsrBI CCGCTC 1 cut(s) 72
BsrGI TGTACA 1 cut(s) 174
BsrI ACTGG 2 cut(s) 454, 506
BssECI CCNNGG 3 cut(s) 219, 312, 548
BssMI GATC 3 cut(s) 66, 153, 271
BssSI CACGAG 1 cut(s) 623
BssT1I CCWWGG 2 cut(s) 312, 548
Bst2BI CACGAG 1 cut(s) 623
Bst4CI ACNGT 3 cut(s) 223, 349, 408
Bst6I CTCTTC 1 cut(s) 330
BstAFI CTTAAG 1 cut(s) 632
BstAUI TGTACA 1 cut(s) 174
BstC8I GCNNGC 2 cut(s) 321, 339
BstDEI CTNAG 1 cut(s) 169
BstDSI CCRYGG 1 cut(s) 219
BstFNI CGCG 1 cut(s) 337
BstKTI GATC 3 cut(s) 69, 156, 274
BstMAI GTCTC 1 cut(s) 18
BstMBI GATC 3 cut(s) 66, 153, 271
BstMWI GCNNNNNNNGC 1 cut(s) 581
BstSFI CTRYAG 1 cut(s) 419
BstUI CGCG 1 cut(s) 337
BstX2I RGATCY 1 cut(s) 66
BstYI RGATCY 1 cut(s) 66
BsuI GTATCC 1 cut(s) 537
BsuRI GGCC 1 cut(s) 561
BtgI CCRYGG 1 cut(s) 219
BtsIMutI CAGTG 1 cut(s) 177
Cac8I GCNNGC 2 cut(s) 321, 339
Cfr13I GGNCC 1 cut(s) 52
Csp6I GTAC 2 cut(s) 175, 238
CviAII CATG 1 cut(s) 232
CviJI RGCY 9 cut(s) 20, 193, 319, 323, 389, 561, 612, 631, 637
CviKI_1 RGCY 9 cut(s) 20, 193, 319, 323, 389, 561, 612, 631, 637
CviQI GTAC 2 cut(s) 175, 238
DdeI CTNAG 1 cut(s) 169
DpnI GATC 3 cut(s) 68, 155, 273
DpnII GATC 3 cut(s) 66, 153, 271
DrdI GACNNNNNNGTC 1 cut(s) 155
DseDI GACNNNNNNGTC 1 cut(s) 155
Eam1104I CTCTTC 1 cut(s) 330
EarI CTCTTC 1 cut(s) 330
Eco130I CCWWGG 2 cut(s) 312, 548
Eco147I AGGCCT 1 cut(s) 561
Eco47I GGWCC 1 cut(s) 52
EcoT14I CCWWGG 2 cut(s) 312, 548
ErhI CCWWGG 2 cut(s) 312, 548
FaeI CATG 1 cut(s) 235
FalI AAGNNNNNCTT 2 cut(s) 182, 214
FatI CATG 1 cut(s) 231
GsuI CTGGAG 1 cut(s) 42
HaeIII GGCC 1 cut(s) 561
Hin1II CATG 1 cut(s) 235
HincII GTYRAC 1 cut(s) 499
HindII GTYRAC 1 cut(s) 499
HindIII AAGCTT 1 cut(s) 635
HinfI GANTC 2 cut(s) 4, 331
Hpy166II GTNNAC 2 cut(s) 175, 499
Hpy188I TCNGA 4 cut(s) 206, 287, 330, 570
Hpy188III TCNNGA 6 cut(s) 64, 110, 275, 383, 429, 607
Hpy8I GTNNAC 2 cut(s) 175, 499
HpyAV CCTTC 2 cut(s) 476, 610
HpyCH4III ACNGT 3 cut(s) 223, 349, 408
HpyCH4V TGCA 2 cut(s) 89, 421
HpyF10VI GCNNNNNNNGC 1 cut(s) 581
HpyF3I CTNAG 1 cut(s) 169
Hsp92II CATG 1 cut(s) 235
KpnI GGTACC 1 cut(s) 241
Kzo9I GATC 3 cut(s) 66, 153, 271
LguI GCTCTTC 1 cut(s) 330
LmnI GCTCC 2 cut(s) 77, 628
MalI GATC 3 cut(s) 68, 155, 273
MbiI CCGCTC 1 cut(s) 72
MboI GATC 3 cut(s) 66, 153, 271
MboII GAAGA 2 cut(s) 317, 508
MflI RGATCY 1 cut(s) 66
MluCI AATT 3 cut(s) 93, 351, 454
MnlI CCTC 5 cut(s) 71, 228, 349, 417, 619
MseI TTAA 3 cut(s) 41, 446, 633
MspCI CTTAAG 1 cut(s) 632
MvnI CGCG 1 cut(s) 337
MwoI GCNNNNNNNGC 1 cut(s) 581
NdeII GATC 3 cut(s) 66, 153, 271
NlaIII CATG 1 cut(s) 235
NlaIV GGNNCC 1 cut(s) 239
PceI AGGCCT 1 cut(s) 561
PciSI GCTCTTC 1 cut(s) 330
PfeI GAWTC 2 cut(s) 4, 331
PflMI CCANNNNNTGG 1 cut(s) 225
PspN4I GGNNCC 1 cut(s) 239
PspPI GGNCC 1 cut(s) 52
PsrI GAACNNNNNNTAC 2 cut(s) 342, 374
PstI CTGCAG 1 cut(s) 423
PsuI RGATCY 1 cut(s) 66
RsaI GTAC 2 cut(s) 176, 239
RsaNI GTAC 2 cut(s) 175, 238
SapI GCTCTTC 1 cut(s) 330
SaqAI TTAA 3 cut(s) 41, 446, 633
Sau3AI GATC 3 cut(s) 66, 153, 271
Sau96I GGNCC 1 cut(s) 52
SfcI CTRYAG 1 cut(s) 419
SinI GGWCC 1 cut(s) 52
SmlI CTYRAG 3 cut(s) 62, 108, 632
SmoI CTYRAG 3 cut(s) 62, 108, 632
Sse9I AATT 3 cut(s) 93, 351, 454
SseBI AGGCCT 1 cut(s) 561
SsiI CCGC 3 cut(s) 70, 335, 642
StuI AGGCCT 1 cut(s) 561
StyI CCWWGG 2 cut(s) 312, 548
TaaI ACNGT 3 cut(s) 223, 349, 408
TaqI TCGA 1 cut(s) 139
TasI AATT 3 cut(s) 93, 351, 454
TatI WGTACW 1 cut(s) 174
TfiI GAWTC 2 cut(s) 4, 331
Tru1I TTAA 3 cut(s) 41, 446, 633
Tru9I TTAA 3 cut(s) 41, 446, 633
TscAI CASTG 1 cut(s) 177
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 177
Van91I CCANNNNNTGG 1 cut(s) 225
Vha464I CTTAAG 1 cut(s) 632
VpaK11BI GGWCC 1 cut(s) 52
XapI RAATTY 1 cut(s) 93
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.