Rh4CG202300

ER lumen

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0001878
Physical Location & Seq
Reverse (-)
42832575 .. 42837745
5171 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG202300.1

Sequence Viewer

Length: 648 bp
ATGAATATATTCAGATTAGCGGGAGATATGACGCATTTGGCCAGTGTTCTGGTTTTGCTTCTCAAGATCCACACTATCAAGTCATGTGCTGGCGTGTCCTTGAAGACTCAGGAACTCTATGCTCTTGTCTTTGCTACCCGCTATTTGGATATTTTTGTTAGCTTCTACTCAGTTTATAATACCTTGATGAAGTTGATATTCTTGGGTAGCTCTTTCTCAATTGTTTGGTATATACGGCACCACAGGATTGTTCGTAGATCATATGATAAAGACCAAGACACATTTCGCCATCTTTTTCTGTTACTCCCATGCCTACTTTTGGCCCTGGTCATCAATGAGAAATTCACTTTTAGAGAGGTGATGTGGGCTTTTTCCATATATTTGGAAGCAGTCGCCATACTTCCTCAGCTGGTGCTGTTGCAAAGGACAAGAAATATTGACAACCTAACAGGGCAATATGTAGTCCTCCTCGGGGCCTATCGAGCATTGTACATATTGAACTGGATTTACCGCTACTTTACTGAGGATCACTATGTCCACTGGATAACTTGGGTAGCAGGGATAATCCAGACCTTACTCTATGCTGATTTCTTCTACTATTACTTCCAGAGTTGGAAGAACAATGTAAAGCTTGAGCTGCCAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05110 map05110
Pfam Domains
Protein Families
ER_lumen_recept

Protein Analysis

215

Amino Acids

25.58

Weight (kDa)

9.24

Isoelectric Point (pI)

35.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ER_lumen_recept PF00810 28 - 171 2.4e-58 ER lumen protein retaining receptor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 177
AccB1I GGYRCC 1 cut(s) 237
AciI CCGC 3 cut(s) 20, 139, 511
AclWI GGATC 2 cut(s) 61, 534
AcoI YGGCCR 1 cut(s) 39
AcsI RAATTY 1 cut(s) 341
AfaI GTAC 1 cut(s) 491
AfiI CCNNNNNNNGG 3 cut(s) 145, 319, 472
AgsI TTSAA 2 cut(s) 103, 499
AjnI CCWGG 1 cut(s) 324
AjuI GAANNNNNNNTTGG 2 cut(s) 267, 299
AluBI AGCT 6 cut(s) 162, 210, 409, 631, 637, 644
AluI AGCT 6 cut(s) 162, 210, 409, 631, 637, 644
AlwI GGATC 2 cut(s) 61, 534
Ama87I CYCGRG 1 cut(s) 470
AoxI GGCC 3 cut(s) 39, 321, 474
ApeKI GCWGC 1 cut(s) 637
ApoI RAATTY 1 cut(s) 341
Asp700I GAANNNNTTC 1 cut(s) 8
AspS9I GGNCC 2 cut(s) 322, 474
AsuHPI GGTGA 1 cut(s) 370
AvaI CYCGRG 1 cut(s) 470
BalI TGGCCA 1 cut(s) 41
BanI GGYRCC 1 cut(s) 237
BbsI GAAGAC 1 cut(s) 110
BbvCI CCTCAGC 1 cut(s) 405
BbvI GCAGC 1 cut(s) 624
BccI CCATC 1 cut(s) 297
BceAI ACGGC 1 cut(s) 251
BciT130I CCWGG 1 cut(s) 326
BisI GCNGC 1 cut(s) 638
BlsI GCNGC 1 cut(s) 639
Bme1390I CCNGG 1 cut(s) 326
BmeT110I CYCGRG 1 cut(s) 470
BmgT120I GGNCC 2 cut(s) 322, 474
BmiI GGNNCC 2 cut(s) 239, 475
BmrFI CCNGG 1 cut(s) 326
BpiI GAAGAC 1 cut(s) 110
Bpu10I CCTNAGC 1 cut(s) 405
BpuEI CTTGAG 1 cut(s) 47
BsaJI CCNNGG 2 cut(s) 324, 469
Bsc4I CCNNNNNNNGG 3 cut(s) 145, 319, 472
Bse1I ACTGG 3 cut(s) 42, 506, 545
BseBI CCWGG 1 cut(s) 326
BseDI CCNNGG 2 cut(s) 324, 469
BseLI CCNNNNNNNGG 3 cut(s) 145, 319, 472
BseMII CTCAG 4 cut(s) 122, 183, 419, 513
BseNI ACTGG 3 cut(s) 42, 506, 545
BseRI GAGGAG 1 cut(s) 458
BseXI GCAGC 1 cut(s) 624
BshFI GGCC 3 cut(s) 41, 323, 476
BshNI GGYRCC 1 cut(s) 237
BsiHKCI CYCGRG 1 cut(s) 470
BslI CCNNNNNNNGG 3 cut(s) 145, 319, 472
BsnI GGCC 3 cut(s) 41, 323, 476
BsoBI CYCGRG 1 cut(s) 470
Bsp1407I TGTACA 1 cut(s) 489
Bsp143I GATC 3 cut(s) 66, 257, 526
BspACI CCGC 3 cut(s) 20, 139, 511
BspANI GGCC 3 cut(s) 41, 323, 476
BspCNI CTCAG 4 cut(s) 121, 182, 418, 514
BspLI GGNNCC 2 cut(s) 239, 475
BspPI GGATC 2 cut(s) 61, 534
BspT107I GGYRCC 1 cut(s) 237
BsrGI TGTACA 1 cut(s) 489
BsrI ACTGG 3 cut(s) 42, 506, 545
BssECI CCNNGG 2 cut(s) 324, 469
BssMI GATC 3 cut(s) 66, 257, 526
Bst2UI CCWGG 1 cut(s) 326
BstAUI TGTACA 1 cut(s) 489
BstC8I GCNNGC 2 cut(s) 91, 642
BstDEI CTNAG 4 cut(s) 108, 169, 405, 522
BstKTI GATC 3 cut(s) 69, 260, 529
BstMBI GATC 3 cut(s) 66, 257, 526
BstMWI GCNNNNNNNGC 2 cut(s) 482, 637
BstNI CCWGG 1 cut(s) 326
BstSCI CCNGG 1 cut(s) 324
BstV1I GCAGC 1 cut(s) 624
BstV2I GAAGAC 1 cut(s) 110
BstX2I RGATCY 1 cut(s) 66
BstXI CCANNNNNNTGG 2 cut(s) 49, 382
BstYI RGATCY 1 cut(s) 66
BsuRI GGCC 3 cut(s) 41, 323, 476
BtsIMutI CAGTG 2 cut(s) 49, 538
Cac8I GCNNGC 2 cut(s) 91, 642
Cfr13I GGNCC 2 cut(s) 322, 474
CseI GACGC 1 cut(s) 40
Csp6I GTAC 1 cut(s) 490
CviAII CATG 2 cut(s) 84, 309
CviQI GTAC 1 cut(s) 490
DdeI CTNAG 4 cut(s) 108, 169, 405, 522
DpnI GATC 3 cut(s) 68, 259, 528
DpnII GATC 3 cut(s) 66, 257, 526
EaeI YGGCCR 1 cut(s) 39
Eco88I CYCGRG 1 cut(s) 470
EcoO109I RGGNCCY 1 cut(s) 474
EcoRII CCWGG 1 cut(s) 324
FaeI CATG 2 cut(s) 87, 312
FatI CATG 2 cut(s) 83, 308
FauI CCCGC 2 cut(s) 13, 146
FauNDI CATATG 1 cut(s) 262
Fnu4HI GCNGC 1 cut(s) 638
Fsp4HI GCNGC 1 cut(s) 638
GluI GCNGC 1 cut(s) 638
HaeIII GGCC 3 cut(s) 41, 323, 476
HgaI GACGC 1 cut(s) 40
Hin1II CATG 2 cut(s) 87, 312
HindIII AAGCTT 1 cut(s) 629
HinfI GANTC 1 cut(s) 106
HphI GGTGA 1 cut(s) 370
Hpy166II GTNNAC 1 cut(s) 538
Hpy188I TCNGA 1 cut(s) 14
Hpy188III TCNNGA 4 cut(s) 64, 110, 568, 607
Hpy8I GTNNAC 1 cut(s) 538
HpyCH4V TGCA 1 cut(s) 421
HpyF10VI GCNNNNNNNGC 2 cut(s) 482, 637
HpyF3I CTNAG 4 cut(s) 108, 169, 405, 522
Hsp92II CATG 2 cut(s) 87, 312
Kzo9I GATC 3 cut(s) 66, 257, 526
Lsp1109I GCAGC 1 cut(s) 624
MaeIII GTNAC 1 cut(s) 300
MalI GATC 3 cut(s) 68, 259, 528
MboI GATC 3 cut(s) 66, 257, 526
MboII GAAGA 3 cut(s) 115, 583, 628
MfeI CAATTG 1 cut(s) 219
MflI RGATCY 1 cut(s) 66
MlsI TGGCCA 1 cut(s) 41
MluCI AATT 2 cut(s) 219, 341
MluNI TGGCCA 1 cut(s) 41
MlyI GAGTC 1 cut(s) 100
MmeI TCCRAC 1 cut(s) 593
MnlI CCTC 5 cut(s) 349, 414, 476, 479, 517
Mox20I TGGCCA 1 cut(s) 41
MroXI GAANNNNTTC 1 cut(s) 8
MscI TGGCCA 1 cut(s) 41
Msp20I TGGCCA 1 cut(s) 41
MspA1I CMGCKG 1 cut(s) 409
MspR9I CCNGG 1 cut(s) 326
MunI CAATTG 1 cut(s) 219
MvaI CCWGG 1 cut(s) 326
MwoI GCNNNNNNNGC 2 cut(s) 482, 637
NdeI CATATG 1 cut(s) 262
NdeII GATC 3 cut(s) 66, 257, 526
NlaIII CATG 2 cut(s) 87, 312
NlaIV GGNNCC 2 cut(s) 239, 475
PdmI GAANNNNTTC 1 cut(s) 8
PkrI GCNGC 1 cut(s) 639
PleI GAGTC 1 cut(s) 100
PpsI GAGTC 1 cut(s) 100
PsiI TTATAA 1 cut(s) 177
Psp6I CCWGG 1 cut(s) 324
PspGI CCWGG 1 cut(s) 324
PspN4I GGNNCC 2 cut(s) 239, 475
PspPI GGNCC 2 cut(s) 322, 474
PsrI GAACNNNNNNTAC 2 cut(s) 491, 523
PsuI RGATCY 1 cut(s) 66
PvuII CAGCTG 1 cut(s) 409
RsaI GTAC 1 cut(s) 491
RsaNI GTAC 1 cut(s) 490
SatI GCNGC 1 cut(s) 638
Sau3AI GATC 3 cut(s) 66, 257, 526
Sau96I GGNCC 2 cut(s) 322, 474
SchI GAGTC 1 cut(s) 100
ScrFI CCNGG 1 cut(s) 326
SmlI CTYRAG 2 cut(s) 62, 632
SmoI CTYRAG 2 cut(s) 62, 632
Sse9I AATT 2 cut(s) 219, 341
SsiI CCGC 3 cut(s) 20, 139, 511
SspI AATATT 1 cut(s) 436
StyD4I CCNGG 1 cut(s) 324
TaqI TCGA 1 cut(s) 481
TasI AATT 2 cut(s) 219, 341
TatI WGTACW 1 cut(s) 489
TscAI CASTG 2 cut(s) 49, 545
TseI GCWGC 1 cut(s) 637
TspDTI ATGAA 2 cut(s) 17, 203
TspRI CASTG 2 cut(s) 49, 545
XapI RAATTY 1 cut(s) 341
XmnI GAANNNNTTC 1 cut(s) 8
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.