MD04G1009900.v1.1

ER lumen

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0001878
Physical Location & Seq
Forward (+)
1117216 .. 1119931
2716 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1009900.v1.1.491

Sequence Viewer

Length: 648 bp
ATGAATATATTCAGATTAGCAGGGGATATGACCCATTTGGCCAGCGTTCTGGTTTTGCTTCTCAAGATCCACACTATCAAATCCTGTTCTGGTGTTTCCTTGAAGACTCAAGAACTCTATGCTCTTGTCTTTGCAACCCGCTATTTGGATATTTTTGTTACCTACGTCTCGTTTTATAATACTGTGATGAAGTTGATATTTTTGGGTAGCTCTTTCTCAATTGTTTGGTACATAAGGCGCCACAGGGTTGTTCGGAGATCATATGATAAGGACCAAGACACATTCCGCCATTTATTTCTTCTGCTCCCTTGTCTTGTTTTGGCCCTGGTTATAAATGAGAAGTTCACTTTTAAAGAGGTGATGTGGACGTTTTCCATATATTTGGAAGCAGTTGCCATACTTCCTCAACTGGTGCTGTTGCAAAGGACAAGAAACATTGATAACTTAACAGGGCAATATGTTTTCCTTCTTGGAGCATATCGAGCATTATACATATTGAACTGGATTTATCGCTACTTTACTGAGGAACACTATGTCCACTGGATAACTTGGATCGCAGGGATTATCCAGACTTTGCTCTATGCTGATTTCTTCTACTATTACTTCCAGAGCTGGAAGAACAGCGTAAAGCTCGAGCTACCAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05110 map05110
Pfam Domains
Protein Families
ER_lumen_recept

Protein Analysis

216

Amino Acids

25.64

Weight (kDa)

9.33

Isoelectric Point (pI)

39.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ER_lumen_recept PF00810 28 - 171 4.7e-59 ER lumen protein retaining receptor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 177, 332
AccB1I GGYRCC 1 cut(s) 237
AciI CCGC 2 cut(s) 139, 286
AclWI GGATC 2 cut(s) 61, 560
AcoI YGGCCR 1 cut(s) 39
AcyI GRCGYC 1 cut(s) 238
AfaI GTAC 1 cut(s) 230
AfiI CCNNNNNNNGG 1 cut(s) 145
AgsI TTSAA 2 cut(s) 103, 499
AjnI CCWGG 1 cut(s) 324
AloI GAACNNNNNNTCC 2 cut(s) 519, 551
AluBI AGCT 5 cut(s) 210, 612, 631, 637, 644
AluI AGCT 5 cut(s) 210, 612, 631, 637, 644
Alw26I GTCTC 1 cut(s) 172
AlwI GGATC 2 cut(s) 61, 560
Ama87I CYCGRG 1 cut(s) 632
AoxI GGCC 2 cut(s) 39, 321
Asp700I GAANNNNTTC 1 cut(s) 8
AspLEI GCGC 1 cut(s) 240
AspS9I GGNCC 2 cut(s) 271, 322
AsuHPI GGTGA 1 cut(s) 370
AvaI CYCGRG 1 cut(s) 632
AvaII GGWCC 1 cut(s) 271
BalI TGGCCA 1 cut(s) 41
BanI GGYRCC 1 cut(s) 237
BbsI GAAGAC 1 cut(s) 110
BciT130I CCWGG 1 cut(s) 326
BcoDI GTCTC 1 cut(s) 172
BfoI RGCGCY 1 cut(s) 241
Bme1390I CCNGG 1 cut(s) 326
Bme18I GGWCC 1 cut(s) 271
BmeT110I CYCGRG 1 cut(s) 632
BmgT120I GGNCC 2 cut(s) 271, 322
BmiI GGNNCC 1 cut(s) 239
BmrFI CCNGG 1 cut(s) 326
BpiI GAAGAC 1 cut(s) 110
BpuEI CTTGAG 2 cut(s) 47, 93
BsaHI GRCGYC 1 cut(s) 238
BsaJI CCNNGG 1 cut(s) 324
Bsc4I CCNNNNNNNGG 1 cut(s) 145
Bse1I ACTGG 3 cut(s) 414, 506, 545
BseBI CCWGG 1 cut(s) 326
BseDI CCNNGG 1 cut(s) 324
BseLI CCNNNNNNNGG 1 cut(s) 145
BseMII CTCAG 1 cut(s) 513
BseNI ACTGG 3 cut(s) 414, 506, 545
BshFI GGCC 2 cut(s) 41, 323
BshNI GGYRCC 1 cut(s) 237
BsiHKCI CYCGRG 1 cut(s) 632
BslI CCNNNNNNNGG 1 cut(s) 145
BsmAI GTCTC 1 cut(s) 172
BsmBI CGTCTC 1 cut(s) 172
BsnI GGCC 2 cut(s) 41, 323
BsoBI CYCGRG 1 cut(s) 632
Bsp143I GATC 3 cut(s) 66, 257, 552
BspACI CCGC 2 cut(s) 139, 286
BspANI GGCC 2 cut(s) 41, 323
BspCNI CTCAG 1 cut(s) 514
BspLI GGNNCC 1 cut(s) 239
BspPI GGATC 2 cut(s) 61, 560
BspT107I GGYRCC 1 cut(s) 237
BsrI ACTGG 3 cut(s) 414, 506, 545
BssECI CCNNGG 1 cut(s) 324
BssMI GATC 3 cut(s) 66, 257, 552
BssNI GRCGYC 1 cut(s) 238
Bst2UI CCWGG 1 cut(s) 326
Bst4CI ACNGT 1 cut(s) 184
BstACI GRCGYC 1 cut(s) 238
BstC8I GCNNGC 1 cut(s) 43
BstDEI CTNAG 1 cut(s) 522
BstH2I RGCGCY 1 cut(s) 241
BstHHI GCGC 1 cut(s) 240
BstKTI GATC 3 cut(s) 69, 260, 555
BstMAI GTCTC 1 cut(s) 172
BstMBI GATC 3 cut(s) 66, 257, 552
BstMWI GCNNNNNNNGC 1 cut(s) 482
BstNI CCWGG 1 cut(s) 326
BstSCI CCNGG 1 cut(s) 324
BstV2I GAAGAC 1 cut(s) 110
BstX2I RGATCY 1 cut(s) 66
BstXI CCANNNNNNTGG 2 cut(s) 49, 382
BstYI RGATCY 1 cut(s) 66
BsuRI GGCC 2 cut(s) 41, 323
BtsIMutI CAGTG 1 cut(s) 538
Cac8I GCNNGC 1 cut(s) 43
CfoI GCGC 1 cut(s) 240
Cfr13I GGNCC 2 cut(s) 271, 322
Csp6I GTAC 1 cut(s) 229
CviJI RGCY 7 cut(s) 41, 210, 323, 612, 631, 637, 644
CviKI_1 RGCY 7 cut(s) 41, 210, 323, 612, 631, 637, 644
CviQI GTAC 1 cut(s) 229
DdeI CTNAG 1 cut(s) 522
DinI GGCGCC 1 cut(s) 239
DpnI GATC 3 cut(s) 68, 259, 554
DpnII GATC 3 cut(s) 66, 257, 552
DraI TTTAAA 1 cut(s) 352
EaeI YGGCCR 1 cut(s) 39
EciI GGCGGA 1 cut(s) 275
Eco47I GGWCC 1 cut(s) 271
Eco88I CYCGRG 1 cut(s) 632
EcoRII CCWGG 1 cut(s) 324
EgeI GGCGCC 1 cut(s) 239
EheI GGCGCC 1 cut(s) 239
Esp3I CGTCTC 1 cut(s) 172
FauI CCCGC 1 cut(s) 146
FauNDI CATATG 1 cut(s) 262
GlaI GCGC 1 cut(s) 239
HaeII RGCGCY 1 cut(s) 241
HaeIII GGCC 2 cut(s) 41, 323
HhaI GCGC 1 cut(s) 240
Hin1I GRCGYC 1 cut(s) 238
Hin6I GCGC 1 cut(s) 238
HinP1I GCGC 1 cut(s) 238
HinfI GANTC 1 cut(s) 106
HphI GGTGA 1 cut(s) 370
Hpy166II GTNNAC 3 cut(s) 345, 366, 538
Hpy188I TCNGA 2 cut(s) 14, 255
Hpy188III TCNNGA 4 cut(s) 64, 110, 568, 607
Hpy8I GTNNAC 3 cut(s) 345, 366, 538
HpyAV CCTTC 1 cut(s) 476
HpyCH4III ACNGT 1 cut(s) 184
HpyCH4IV ACGT 2 cut(s) 165, 368
HpyCH4V TGCA 2 cut(s) 134, 421
HpyF10VI GCNNNNNNNGC 1 cut(s) 482
HpyF3I CTNAG 1 cut(s) 522
HpySE526I ACGT 2 cut(s) 165, 368
Hsp92I GRCGYC 1 cut(s) 238
HspAI GCGC 1 cut(s) 238
KasI GGCGCC 1 cut(s) 237
Kzo9I GATC 3 cut(s) 66, 257, 552
LmnI GCTCC 2 cut(s) 309, 473
MaeII ACGT 2 cut(s) 165, 368
MaeIII GTNAC 1 cut(s) 157
MalI GATC 3 cut(s) 68, 259, 554
MboI GATC 3 cut(s) 66, 257, 552
MboII GAAGA 4 cut(s) 115, 290, 583, 628
MfeI CAATTG 1 cut(s) 219
MflI RGATCY 1 cut(s) 66
MlsI TGGCCA 1 cut(s) 41
MluCI AATT 1 cut(s) 219
MluNI TGGCCA 1 cut(s) 41
Mly113I GGCGCC 1 cut(s) 238
MlyI GAGTC 1 cut(s) 100
MnlI CCTC 3 cut(s) 349, 414, 517
Mox20I TGGCCA 1 cut(s) 41
MroXI GAANNNNTTC 1 cut(s) 8
MscI TGGCCA 1 cut(s) 41
MseI TTAA 2 cut(s) 351, 446
Msp20I TGGCCA 1 cut(s) 41
MspR9I CCNGG 1 cut(s) 326
MunI CAATTG 1 cut(s) 219
MvaI CCWGG 1 cut(s) 326
MwoI GCNNNNNNNGC 1 cut(s) 482
NarI GGCGCC 1 cut(s) 238
NdeI CATATG 1 cut(s) 262
NdeII GATC 3 cut(s) 66, 257, 552
NlaIV GGNNCC 1 cut(s) 239
PaeR7I CTCGAG 1 cut(s) 632
PdmI GAANNNNTTC 1 cut(s) 8
PleI GAGTC 1 cut(s) 100
PluTI GGCGCC 1 cut(s) 241
PpsI GAGTC 1 cut(s) 100
PsiI TTATAA 2 cut(s) 177, 332
Psp6I CCWGG 1 cut(s) 324
PspGI CCWGG 1 cut(s) 324
PspN4I GGNNCC 1 cut(s) 239
PspPI GGNCC 2 cut(s) 271, 322
PspXI VCTCGAGB 1 cut(s) 632
PsuI RGATCY 1 cut(s) 66
RsaI GTAC 1 cut(s) 230
RsaNI GTAC 1 cut(s) 229
SaqAI TTAA 2 cut(s) 351, 446
Sau3AI GATC 3 cut(s) 66, 257, 552
Sau96I GGNCC 2 cut(s) 271, 322
SchI GAGTC 1 cut(s) 100
ScrFI CCNGG 1 cut(s) 326
SetI ASST 9 cut(s) 164, 168, 212, 360, 371, 614, 633, 639, 646
SfoI GGCGCC 1 cut(s) 239
Sfr274I CTCGAG 1 cut(s) 632
SinI GGWCC 1 cut(s) 271
SlaI CTCGAG 1 cut(s) 632
SmlI CTYRAG 3 cut(s) 62, 108, 632
SmoI CTYRAG 3 cut(s) 62, 108, 632
Sse9I AATT 1 cut(s) 219
SsiI CCGC 2 cut(s) 139, 286
SspDI GGCGCC 1 cut(s) 237
StyD4I CCNGG 1 cut(s) 324
TaaI ACNGT 1 cut(s) 184
TaiI ACGT 2 cut(s) 168, 371
TaqI TCGA 2 cut(s) 481, 633
TasI AATT 1 cut(s) 219
Tru1I TTAA 2 cut(s) 351, 446
Tru9I TTAA 2 cut(s) 351, 446
TscAI CASTG 1 cut(s) 545
TspDTI ATGAA 2 cut(s) 17, 203
TspRI CASTG 1 cut(s) 545
VpaK11BI GGWCC 1 cut(s) 271
XhoI CTCGAG 1 cut(s) 632
XmnI GAANNNNTTC 1 cut(s) 8
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.